LOCUS       NZ_LN850107            22743 bp    DNA     linear   CON 13-AUG-2024
DEFINITION  Alloactinosynnema sp. L-07 isolate Alloactinosynnema sp. L-07
            chromosome I.
ACCESSION   NZ_LN850107
VERSION     NZ_LN850107.1
KEYWORDS    RefSeq.
SOURCE      Alloactinosynnema sp. L-07
  ORGANISM  Alloactinosynnema sp. L-07
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1
  AUTHORS   Ramaraj,Thiru.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2015) National Center for Genome Resources (NCGR),
            National Center for Genome Resources (NCGR), 2935 Rodeo Park Drive
            East, Santa Fe, New Mexico, 87505, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900070365.1-RS_2024_08_13
            Annotation Date                   :: 08/13/2024 03:00:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,999
            CDSs (total)                      :: 6,934
            Genes (coding)                    :: 6,831
            CDSs (with protein)               :: 6,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 30 of 103
            Pseudo Genes (incomplete)         :: 83 of 103
            Pseudo Genes (internal stop)      :: 1 of 103
            Pseudo Genes (multiple problems)  :: 11 of 103
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:22:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1097431
            Orig. end                         :: 1120174
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LN850107.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..22743
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="redox-cofactor"
                     /region_number="8"
                     /rules="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /tool="antismash"
     cand_cluster    1..22743
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cds(PF04055 and (TIGR04085 or SPASM))
                     and (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /kind="single"
                     /product="redox-cofactor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22743
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12743](+)"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12743
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
     gene            complement(292..1209)
                     /locus_tag="BN1701_RS05290"
     CDS             complement(292..1209)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BN1701_RS05290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_067520526.1"
                     /transl_table=11
                     /translation="MRTPRSASGTGSEPGPSFAFTLRKAIRERGISMERICERLRERGT
                     PVSVATLSYWQSGRSQPERAASLAAVEQLEQLLNLRTGDLLGMLSAPRPRGRSVNREAI
                     DVAALWPEPEAVHSALANVDTSWDTRLSRVSLHDRIVIGPDGTEQTQSIRQVLRAEEDG
                     VDRWVVIYEVDEPGGPMPLIRPVWNCSLGTVNVDQATGLLVTELVFPHPLARGESILTE
                     HALDNPPGQPLSVQHERRCRLLVRELLLEVRFDRAALPARIVQYSVIADHERVKTLSLD
                     AEGSVHALATDFGPGRYGIRWEWD"
     gene            complement(1314..3185)
                     /locus_tag="BN1701_RS05295"
     CDS             complement(1314..3185)
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075: alkaline serine protease, subtilase family"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /locus_tag="BN1701_RS05295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="S8 family serine peptidase"
                     /protein_id="WP_197672049.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 1.1e-44, bitscore:
                     151.5, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGRRIALNIIIALAASSVAIVAAPVANAEQPGRAYVENVADGDIV
                     DQYIVTYKPQSQARRDKAKRVASQRAAGTKHGVAVEELRDTALGSQVVRTDRGLDAAQA
                     RGFLRTLAAQPDVEAVEPDSRFYPTYSPNDTYYGDQWHYWESTAGMRLPTAWDTANGAG
                     SVVAVIDTGRTFHPDLDANTLPGYDFISSSSAARDGNGRDSNPQDQGDWVPNAGDCGVG
                     SNPSDSSWHGTHVAGTIAAVTGNGSGVAGVAPNAKVLHARVLGFCGGALSDIADAIIWA
                     SGGSVPGVPANANPAKVINMSLGGGGACGTTYQNAINSAVGRGSSVIVAAGNSNIDAAN
                     TRPANCGGVITVAATDRQGNRASYSNYGAVIDVSAPGGETATATNGVLSAMNAGTTTPG
                     GSTYEYYQGTSMATPHVAGLVALMLGEKSMSPASVESSLKANSRPLPGSCSGGCGAGLV
                     DAAATIASLGGGSGQVLANPGFESGNTSWTASSGVITTDATYPARTGSWKAWLCGYGAT
                     HTDTVDQTVSIPSGATSSTLSFYLRIDSAETGTTVYDTLKVQILSTSGAVLGSVTYSNA
                     DENTAYALKSINMNSYIGQTVKIRFTATEDADLQTSFVIDDTALTYS"
     misc_feature    complement(3152..3166)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.21"
                     /tool="antismash"
     misc_feature    3153..3166
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.77"
                     /tool="antismash"
     misc_feature    complement(3153..3168)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.84"
                     /tool="antismash"
     gene            complement(3475..5358)
                     /locus_tag="BN1701_RS37105"
     CDS             complement(3475..5358)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /locus_tag="BN1701_RS37105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AAA family ATPase"
                     /protein_id="WP_231949491.1"
                     /transl_table=11
                     /translation="MANQPSLSTGAAGAAAPMDAVARTDEAQILEAALLSGTHVLLEGP
                     PGTGKSTLLRQVAREREIPFVLVEGNSELTPARLIGHFDPALVLDRGYVAEIFEDGPLL
                     DAMRRGGLLYVEELNRIPEETLNVLLAAMSEKEIAVPRLGRIVAAAGFGLVAAMNPFDT
                     VGTARVSSALYDRTCRISMGYQSAEAERDIVSLHAPGIDASWRNKVVALVRASREHDAL
                     EWGSSVRGAIDIARIAVELARLRGVAVNDWHVGLAAAKVSLSGRIRLHDSAGRSPEDVV
                     LEMYERVFGPEPAQNAADPDPEASPEGTLSPSTPTGSPQPTHSPHARDRPRGERTTGRS
                     DLARNPRFAELSPEVGVLDQDALNSALAEHADETLTLIVEMSLATDDALRSQVRRLAPR
                     LILEQTRRGLPKSSGIARRRIRRAVDGGDIDLDRSMDRVLGARAEGRSPRLDELLVSDW
                     ARPELALCLVIDRSGSMNGARLTTAAVAAAACASLTPREHAVITFAAHVGVLKPMNERR
                     PASETVASILSLRGHGTTSLSAALDEAHSQLGKAGARRKVTLLLSDCRATEESGAVAAA
                     LGLQELIILAPGDDVEAARQLARAAGGTWAPVDTVLDVPRLLNRLMADDRV"
     gene            complement(5359..6471)
                     /locus_tag="BN1701_RS05305"
     CDS             complement(5359..6471)
                     /NRPS_PKS="Domain: PKS_ER (21-289). E-value: 9.1e-13.
                     Score: 39.9. Matches aSDomain:
                     nrpspksdomains_BN1701_RS05305_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334013.1"
                     /locus_tag="BN1701_RS05305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="WP_054046044.1"
                     /sec_met_domain="ADH_N (E-value: 7.2e-29, bitscore: 98.5,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 2.9e-20, bitscore:
                     71.3, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTIAAVVYEPGKPIEIEELDLDGPQAGEVLIRYTHAGLCHSDVH
                     VAHGDIQARLPMVLGHEGAGIIEEVGPGVTRVKVGDHVVCSFIPNCGTCRYCANGQQSI
                     CDMGATILEGYLPGDRFPISGPRGQYGAMCMLGTFSQWGVIHQNSCVKVDDDLPLEKAV
                     LVGCGVPTGWGSAVNTAKVQPGDTVAVYGIGGIGINAVQGARLAGAKNIVAIDPLENKR
                     EKAMELGATHAFADAAQAQDKIVELTRGQGADSAILTPDLLTAEIVTSGFNAIGKGGTV
                     VVTGLNKLDEQTIQLPGSILTLFRKTIKGSLFGDCNPTVDIPKILGLYQSGDLKLDEII
                     TRTYRLDQVNEGYDDLLAGKNVRGVVVHEH"
     aSDomain        complement(5605..6408)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BN1701_RS05305_PKS_ER.1"
                     /evalue="9.10E-13"
                     /label="BN1701_RS05305_PKS_ER.1"
                     /locus_tag="BN1701_RS05305"
                     /protein_end="289"
                     /protein_start="21"
                     /score="39.9"
                     /tool="antismash"
                     /translation="LDGPQAGEVLIRYTHAGLCHSDVHVAHGDIQARLPMVLGHEGAGI
                     IEEVGPGVTRVKVGDHVVCSFIPNCGTCRYCANGQQSICDMGATILEGYLPGDRFPISG
                     PRGQYGAMCMLGTFSQWGVIHQNSCVKVDDDLPLEKAVLVGCGVPTGWGSAVNTAKVQP
                     GDTVAVYGIGGIGINAVQGARLAGAKNIVAIDPLENKREKAMELGATHAFADAAQAQDK
                     IVELTRGQGADSAILTPDLLTAEIVTSGFNAIGKGGTVVVTGLNKL"
     misc_feature    6486..6501
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    6564..6577
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.33"
                     /tool="antismash"
     misc_feature    complement(6573..6588)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.55"
                     /tool="antismash"
     gene            6768..8255
                     /locus_tag="BN1701_RS05310"
     CDS             6768..8255
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1112: sigma-54
                     dependent trancsriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /locus_tag="BN1701_RS05310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="WP_231949814.1"
                     /transl_table=11
                     /translation="MPVLEELEENLRGSNYCTLLVDRDCRVVRRWFDDPRLECGFDALN
                     VTLGACLLEENIGTNALGTVMVTRQGISISGHEHFAKSLQGFSCYGHPIRHPLTKRIEG
                     VLDITAIVDQASPLLPPLISRAVHDIEQRLLDGSRTSEKSLLLAFQGAAGLRRRAILAI
                     GEDIVLSNHAANDLLSPGDIALLRALAEESPREEQTVELTLEAGVAVSIEISRVGGACR
                     GALLRVDRVSGDRARAVLHRSQTKLKEVGAPRMVTGVPGTGRSTRARELAAEHPPLKVM
                     RPAAALLEGGAEWACEFEEAMRRPKGTVCVDGIDLLSSELLDLVVDWVDRDPRPDLILT
                     AGPIAGLTGRAAALAGMAMIREELVPLATRRKDIPGLAAAMLSEVAPNRALHLAPSLIQ
                     ALAAQSWPGNLWELKAVISYLAQQRSVGALTVRDLPEQYRSTSLDHPLTALDQAERDVI
                     VTALKAADGNKVRAAEELGISRTTLYARMRTLRITTY"
     misc_feature    complement(8360..8373)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.01"
                     /tool="antismash"
     gene            8360..9865
                     /locus_tag="BN1701_RS05315"
     CDS             8360..9865
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017981326.1"
                     /locus_tag="BN1701_RS05315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="WP_054055637.1"
                     /sec_met_domain="Aldedh (E-value: 6.8e-166, bitscore:
                     551.0, seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTFVLPDLHASVIPAVRDFLGTPKQLFIGGEWVDAASGETFDTVD
                     PATGRVLTTAALGGAEDIDRAVKAARMAFDGEWSLWTPAQRQRLLFQISEAIRDRAEEF
                     AQLESLDNGKSAAVAQAVDVLWVAELFAYYAGWATKIEGRTIPVSVPWAPGAQWHAYTL
                     RQPVGVCGGIVPWNFPLLMAAFKVPAALACGNTTVLKPAEQTPLSALLLAQVMADAGLP
                     DGVFNVVTGYGDAGASLAAHMDVDKIAFTGSTEVGKLVVDAARGNLKKVSLELGGKSPQ
                     VVYADADLNVAVPGTASGFLFNHGQACTSGTRLLVEDSIFEEFTQAVAEVAGASKLGPG
                     LDPTSEVGPLIDGTQLARVTGFIEAGLNDGARALTGGGRHGDEGFYVEPTVLVDVQDSF
                     SVYREEIFGPVVVATPFSAREGVRRQANDTPFGLAASVWTRDVAKAHRTAREINAGTVW
                     INCHNAFDAAMPFGGFKESGWGRELGEGAIDLYTQNKSVNVAL"
     gene            9888..10001
                     /gene="mftA"
                     /locus_tag="BN1701_RS05320"
     CDS             9888..10001
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658597.1"
                     /locus_tag="BN1701_RS05320"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes in
                     Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /product="mycofactocin precursor MftA"
                     /protein_id="WP_082859660.1"
                     /transl_table=11
                     /translation="MNPADMNPPQDTEDAGEELVAEDLIEEVSIDGMCGVY"
     gene            10001..10300
                     /gene="mftB"
                     /locus_tag="BN1701_RS05325"
     CDS             10001..10300
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: Mycofactocin_RRE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310366.1"
                     /locus_tag="BN1701_RS05325"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ, a
                     cofactor that derives entirely from a Tyr and a Glu in the
                     precursor PqqA.; MftB, a small protein, is a peptide
                     chaperone that assists the radical SAM enzyme MftC in
                     performing two modifications to the C-terminal Val-Tyr
                     dipeptide of the mycofactocin precursor peptide, MftA.
                     MftB's role is analogous to the role of PqqD in the
                     biosynthesis of PQQ, a cofactor that derives entirely from
                     a Tyr and a Glu in the precursor PqqA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="WP_054046050.1"
                     /sec_met_domain="Mycofactocin_RRE (E-value: 1.9e-29,
                     bitscore: 100.4, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPDLLLDPMLGQAWTLSPSVALRPEPFGALAYHFGNRKLTFLKRP
                     RLVSVVRALGERPDVRSALIAAGVPEAQHAAYVAALRALAATDMIRPRAEEVTT"
     aSDomain        10034..10270
                     /aSDomain="Mycofactocin_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a mycofactocin
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_BN1701_RS05325_Mycofactocin_RRE.1"
                     /evalue="7.60E-32"
                     /identifier="RREFam016.1"
                     /label="BN1701_RS05325"
                     /locus_tag="BN1701_RS05325"
                     /protein_end="90"
                     /protein_start="11"
                     /score="100.4"
                     /tool="antismash"
                     /translation="QAWTLSPSVALRPEPFGALAYHFGNRKLTFLKRPRLVSVVRALGE
                     RPDVRSALIAAGVPEAQHAAYVAALRALAATDMI"
     gene            10297..11517
                     /gene="mftC"
                     /locus_tag="BN1701_RS05330"
     CDS             10297..11517
                     /EC_number="1.3.98.7"
                     /EC_number="4.1.99.26"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR04085"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499234.1"
                     /locus_tag="BN1701_RS05330"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron carrier
                     mycofactocin from the terminal Val-Tyr dipeptide of the
                     precursor peptide MftA.; MftC is a radical SAM/SPASM enzyme
                     that catalyzes the first two steps in biosynthesis of the
                     electron carrier mycofactocin from the terminal Val-Tyr
                     dipeptide of the precursor peptide MftA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="WP_054046052.1"
                     /sec_met_domain="PTHR43273 (E-value: 7.2e-38, bitscore:
                     129.2, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 1.5e-23, bitscore: 82.6,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 6.2e-08, bitscore: 31.0,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 5.2e-13, bitscore:
                     47.9, seeds: 281, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAVRERPLGGSLIDQFELGLDAPICLTWELTYACNLACAHCLSS
                     SGKRDPRELSTEQCEAVIDELRRMQVFYVNIGGGEPTIRPDFWHLLEYAVDHQVGVKFS
                     TNGVRITPERARFLASTDYIDVQISLDGATAEVNDYVRGPGSYDTALTALRNLHDAGFK
                     DAKISVVCTRENIGQLDEFKALADRFGATLRLTRLRPSGRGADVWDELHPRPEQQKVLY
                     DWLMAHGEDVLTGDSFFHLAAFGESLPGLNLCGAGRVVCLIDPIGDVYACPFAIHANFL
                     AGNLLADGGFQRVWQTSELFTELRSPQTGGACAKCTFFDSCRGGCMAAKFFTGLPLDGP
                     DPECVRGYGETALAGARTIPAASQDHSKTGPTRNQPVMLKLMTRPPVSACSESPLADYS
                     PENGCCS"
     gene            11514..12743
                     /gene="mftD"
                     /locus_tag="BN1701_RS05335"
     CDS             11514..12743
                     /EC_number="1.4.3.26"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR03966"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499235.1"
                     /locus_tag="BN1701_RS05335"
                     /note="MftD, an enzyme found in the mycofactocin
                     biosynthesis locus, performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="pre-mycofactocin synthase MftD"
                     /protein_id="WP_054046054.1"
                     /sec_met_domain="TIGR03966 (E-value: 6.6e-197, bitscore:
                     652.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLENPWKQNPWFESVAVAQRRARKKLPAPVYGALVAGSERGQTM
                     SDNEAAFRELGFAPHVAGQWAERSMATTVLGQEISLPVLISPTGVQAVHPDGEVAVARA
                     AAARGTVMGLSNFASKSVEEVTATGAATFFQMYWTGDRDVVVQRMRRAHAAGVVGLIAT
                     LDWSFSMGRDWGSPEIPEKVDLKTMVRMAPKVLTKPRWLYQFGRTGHIPDLTAPNLAPL
                     GGEAPTFFTAYYEWMTTPPPSWEDVAWMREQWAQISGTPFLLKGVCRVDDAIRARDVGV
                     SGLSVSNHGGNNLDGTPAAIRMVKPIADAVGHDLDVVMDGGIRRGSDVVKAVALGARAV
                     MIGRAYLWGLAANGQAGVENVLDVLRGGIDSALMGLGVASVDELTPEHVLVPAGFHRTL
                     GVRTPEVAGG"
     gene            12740..13402
                     /gene="mftE"
                     /locus_tag="BN1701_RS05340"
     CDS             12740..13402
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499236.1"
                     /locus_tag="BN1701_RS05340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="WP_054046056.1"
                     /transl_table=11
                     /translation="MTGQIADATWPELAQVDLVLVPVGSIEQHGPHLPLDTDTAIATGV
                     ARGVAAALEGAWVAPAVVYGSSGEHQAFPGTSSVGTDALRLMLVELVRSMRTWTRRVLF
                     VNAHGGNLRALDGAVRQLVDEGHDVTWAPCATEEVDLHAGRTETSLMLHLRPDSVRLDR
                     SEAGNTQPLQEILPTLITRGVRAVSANGVLGDPTGATAEEGSRLLARMVRDVLARAT"
     gene            13399..14163
                     /locus_tag="BN1701_RS05345"
     CDS             13399..14163
                     /NRPS_PKS="Domain: PKS_KR (3-165). E-value: 2.4e-09. Score:
                     29.3. Matches aSDomain:
                     nrpspksdomains_BN1701_RS05345_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499237.1"
                     /locus_tag="BN1701_RS05345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="WP_054046058.1"
                     /transl_table=11
                     /translation="MSRVAIVTGAARGIGAATVARLVADGYRVTAVDSCEGAPLATRAD
                     LDAVAAAHGDTVLAHVADVRDRAALAEAVTHTIDRWHRLDVAVASAAVIRGGAPLWETA
                     EADLDLLWDVDAKGVWNTAAVTVPAMLAGADPSGCRFVAVASAAGTHGMFHLAAYNAVK
                     HAVVGIVKGLAADLAATGVTACAVSPGSTDTPMLQATADLYGVTAAELAGHQLIRRPID
                     PAEIAATIAFCASLAGRVVNGGVVHADGGFAS"
     aSDomain        13408..13893
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BN1701_RS05345_PKS_KR.1"
                     /evalue="2.40E-09"
                     /label="BN1701_RS05345_PKS_KR.1"
                     /locus_tag="BN1701_RS05345"
                     /protein_end="165"
                     /protein_start="3"
                     /score="29.3"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VAIVTGAARGIGAATVARLVADGYRVTAVDSCEGAPLATRADLDA
                     VAAAHGDTVLAHVADVRDRAALAEAVTHTIDRWHRLDVAVASAAVIRGGAPLWETAEAD
                     LDLLWDVDAKGVWNTAAVTVPAMLAGADPSGCRFVAVASAAGTHGMFHLAAYNAVKHA"
     CDS_motif       13414..13476
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BN1701_RS05345_0001"
                     /evalue="2.60E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="BN1701_RS05345"
                     /protein_end="26"
                     /protein_start="5"
                     /score="10.5"
                     /tool="antismash"
                     /translation="IVTGAARGIGAATVARLVADG"
     gene            14160..>15536
                     /gene="mftF"
                     /locus_tag="BN1701_RS05350"
                     /pseudo=""
     CDS             14160..>15536
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR03965.1"
                     /locus_tag="BN1701_RS05350"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969).; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /pseudo=""
                     /sec_met_domain="Glycos_transf_2 (E-value: 9.4e-17,
                     bitscore: 60.1, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTSAAARLPDGFEVRIRDDVTRLGDGRVLVGGSPLRAIRLSSGA
                     MGLIDRDRVRVVDPRSGALARRLLDANVADPVLLAMVPADSMTVVIPVRDRPEQLDRAL
                     AALHPLPTVVVDDGSKAPEAVATVAARHGARLVPLQVNLGPAGARNAGLATVQTPYVAF
                     VDSDVDVTADALMRLAQHFADPCVALVGPRVVSVARSARPRWHERYDESASSLDLGVRA
                     CSVRPGAAVAWLPSACVVGRVDLLADGFDGDLRIGEDVDLVWRLVAAGHTVRYDPSVLA
                     RHDTRPTLTGWLGRKFAYGTGGAVLAQRHGAHTAVAQLSPAMALAAGALLVRRRWSVPI
                     AAVCLARGARSLATSLPDPVITPRLVGQISARGLGWALRQESSLLLRHWWPAALAAAVW
                     SRSIRRAIVTATVVDLIAATLEGVRLRPIAAFFGRRIDDLAYGGGLWWGSIRARSPRAL
                     A"
     gene            complement(15479..15676)
                     /locus_tag="BN1701_RS38080"
     CDS             complement(15479..15676)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /locus_tag="BN1701_RS38080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_369800666.1"
                     /transl_table=11
                     /translation="MGGLIALQCTTGNPADLAGLILSGPAVEIAVGSKRGVEIPIERTN
                     RTASARGERARIEPHQRPPP"
     gene            complement(15821..17266)
                     /locus_tag="BN1701_RS05355"
     CDS             complement(15821..17266)
                     /GO_function="GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /locus_tag="BN1701_RS05355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="WP_054046060.1"
                     /transl_table=11
                     /translation="MTGPLSTNEEAWAAASGWNTSDRMNEIEALLWRSERHPRLSSTIT
                     SVLIYDQVPDWDRFFAAHDWASLLIARCRQRVLEPALPVGPPAWVPDENFQLDYHVRRT
                     RLPAPGGLAALLELAQSLAMTPFDRTRPLWEGTLVEGFDGEKAAYVLKLHHSLTDGMGG
                     IQLMTMVQSRTREHTPNKPLPADPLTARETMDASGLATVELTEQARRVPGVLWRALSMG
                     VYAATHPSEVLSDAVRFAGSMRRTLSPPPAAPSPLLTNRSGTSWKFGVLECGLAELRSA
                     AKAAGGSLNDAYVAALLGGLRRYHDRHGTSIDELPMAMPVSLRKADDPLGGNKFAGAFF
                     AAPVGVEDPAERIATIRGTVLSLRTEPALDTFSMVAPVLNRIPSAVGGFVYDQIGSAAD
                     LSASNIPGVPHPVYMAGAKVERLFPFGPLPGVAVMAAMVSHAGVCCFGINMDGSAVQDP
                     DVMMECLDEGLREVLELSTVEGR"
     gene            complement(17263..18279)
                     /locus_tag="BN1701_RS05360"
     CDS             complement(17263..18279)
                     /EC_number="1.1.1.94"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF009098.0"
                     /locus_tag="BN1701_RS05360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="WP_054046062.1"
                     /transl_table=11
                     /translation="MAGRMPSVAVLGGGSWGTTVASLAARNTDTVLWARDPATVADIND
                     NHRNSRYLNDLPLTEALRATAELAEAVGKADVIVLGVPSQHSRSVLEQVAEHVRPWVPV
                     VSLVKGLEQGTRKRMTEIVNEILPGHPAGVLAGPNIAREVVQGYAAAATIAMPEQQQAS
                     MLQDLFRTTRFRVYTTTDVIGVEYAGSLKNVFAIAVGIGDGLEAGDNTRAMVITRALRE
                     LTRLGVALGGQADTFAGLSGMGDLIATCTSPLSRNRTVGVGLAQGKSLDQIAQEMNQVA
                     EGVKTVATVMKLAAERGIDMPIAREMDAVINHGQPVEDTYRGLLRKAPGHEVHGEEW"
     gene            complement(18279..19721)
                     /locus_tag="BN1701_RS05365"
     CDS             complement(18279..19721)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1141: acyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /locus_tag="BN1701_RS05365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HAD-IB family hydrolase"
                     /protein_id="WP_067520537.1"
                     /transl_table=11
                     /translation="MITPEDVVASIHSGPGGPRIGAFFDLDGTVVAGYTASAFWNDRLR
                     SRDIGVGEFTRTMVAALDGNLLGGNPTKIGDVSVGGLRGRGEDELVELGERLFVQKTAG
                     TIRPQARDLVRAHLKMGHTVAFASAATRFQIEPIAADLGVTHVLCTELETEDGILTGRL
                     AGKMLWGEPKARAVRQFARDKGIDLAASHAYANGDEDVAFLASAGLPYAINPHRGLLDA
                     AALQKWPVLRLREPRKAGLRSVLGTVGALAGLNVGLGVGAALGILNRDRKFGINTGIPL
                     ACDSALALAGVKLNVIGEHNLWRARPSIFIGNHQSSLDSIVMGSLLRKDFTAVGKKEAQ
                     YDPRTLIGGLLLDPAFIDRSNPEKAIAELDKLGERIRSGTSIVIFPEGARAPTPALARF
                     KKGAFHIAIQAGVPMVPIVLRNTGELMWRRSKLVHPGTVEVCVLDPIPTDDWKVEDLDR
                     ITAEVRQKFVDTLEKWPDREGD"
     gene            complement(19718..21598)
                     /locus_tag="BN1701_RS05370"
     CDS             complement(19718..21598)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF008883.0"
                     /locus_tag="BN1701_RS05370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lysophospholipid acyltransferase"
                     /protein_id="WP_054046066.1"
                     /transl_table=11
                     /translation="MGEGHPALIGALDRLHARRLLQRAGVLEEAVTPLGSDTREVADLL
                     GDTAADESLASLAQRLGKKPDDVRAEAAGYLREMSATHGDRAGDTWSRFSQWLMRAHDV
                     YVDDESVSRLRALDRKHSLLFLFSHRSYLDGTMVPEVVSSRGISPAYTFGGANLKFFPV
                     GNWISRAGVIFIRRNTSDMPVYRWALRNYIAQLVRNRANLAWSIEGGRTRTGKLRPPVF
                     GILRYIADAVDAVGAAEALVVPVSIVYDQLHEVAMMTTEARGGSKRPEDLRWLVNFARE
                     QRQRLGRAYLDFGEPFSLSERLAELRADEAAAPHVIERVALDVCHRINRATPVTPTAVV
                     SLAMLGADRALSLDEVLATVAPLADYLSSRSWPVAGAANLTDRSTIRRALQEMTSSGVL
                     TCYEGGTETVWGIAPDRHLVAAFYRNTAIHVLVERAIGELALLAAAEDSAVEGTGEVAR
                     TARGEALRLRELLKFEFFFSGRDEFGHELREELALFGDAKVEFDPADLRLRLERARPHV
                     AHLVLRPYLDAYHVVADRLAAWDDEFEEKRFLDECLRVGRQWALQRRVASDESVTLELF
                     RTALRLARHRDLIDSAEPHLAKRRKDFLAEIREAARRAGVVAQLARASSR"
     misc_feature    complement(21616..21631)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 19.26"
                     /tool="antismash"
     misc_feature    complement(21804..21818)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 18.79"
                     /tool="antismash"
     tRNA            complement(21876..21949)
                     /anticodon=(pos:complement(1119344..1119346),aa:Leu,
                     seq:caa)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="BN1701_RS05375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /product="tRNA-Leu"
     gene            complement(21876..21949)
                     /locus_tag="BN1701_RS05375"
     gene            22105..22707
                     /locus_tag="BN1701_RS05380"
     CDS             22105..22707
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005157321.1"
                     /locus_tag="BN1701_RS05380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ANTAR domain-containing response regulator"
                     /protein_id="WP_054046067.1"
                     /transl_table=11
                     /translation="MTELATEAQAAAPRRVLVAEDEALIRLDLVEMLREEGYEVAGEAA
                     DGEEAVRLAGELKPDLVILDVKMPKMDGIDAATTIAGERIAPVVMLTAFSQRELVERAR
                     DAGAMAYLVKPFAKHDLVPAIELAVSRFSELQALETEVAGLTERLETRKVIERAKGLLM
                     SKQGLSEPEAFRWIQRTAMDRRTTMKAVAEAVVENIT"
ORIGIN
        1 agagcgcgga cttcgcgacc atcgagggtc ttgtcgcggc cggactcggc gttgccctgg
       61 taccggagca gttcgccgga ctttccggca ccatcgggat cgccctgaac accccggccg
      121 cccgccgcac ggtgggcctc acgtggcgca gcgaccgtga tctcgcgcct gcggccaccc
      181 gccttctcga cttcatcaag agcacgccga atccgaccca gcccaggtca ggcctggtct
      241 gacctgggct ggcgcaggta tgcctggtga tggagctggt cgcggaggct cctagtccca
      301 ctcccagcgg atgccgtagc ggcctggccc gaagtcggtg gccagggcgt gtacggaccc
      361 ttccgcgtcc aagctcaacg tcttgacccg ctcgtggtcg gcgatcacgc tgtactgcac
      421 gatcctggcg ggcagggcgg cccggtcgaa acgcacctcc agcagcagtt cccggacgag
      481 gagcctgcag cggcgctcgt gttgcaccga cagcggctgc cccggcgggt tgtccagcgc
      541 gtgctcggtg aggatggatt ccccgcgtgc caacggatgc gggaaaacca gctcggtcac
      601 gagcagcccg gtggcctggt cgacgttcac cgtgccgagc gagcagttcc acacgggacg
      661 gatcaggggc atcggcccac ctggctcatc gacctcatag atcacgaccc accggtcgac
      721 gccgtcctcc tcggcgcgca gcacctggcg gatggactgg gtctgttcgg tgccgtccgg
      781 cccgatcacg atccggtcgt gcaggctgac cctgctcagc cgcgtgtccc agctggtgtc
      841 gacgttggcg agggcgctgt gcactgcctc cggctccggc cacagcgccg ccacgtcgat
      901 ggcttcgcgg ttgaccgacc ggccgcgtgg ccgcggggcg gacaacatgc cgagcaggtc
      961 gcccgtccgc aggttgagca gctgctcgag ttgctcgacc gcggccagcg acgccgcgcg
     1021 ttccggctgg ctgcggccgg actgccagta gctcaaggtg gcgacgctga ccggggtgcc
     1081 acgctcgcgg agccgttcac agatccgctc catgctgatc ccacgctccc gaatcgcctt
     1141 gcgcagcgtg aaagcgaagc tcgggcccgg ctcagagccc gtcccgctcg ccgaccgggg
     1201 agttctcacg atgagccacg ttagctgcgc cacctagagt agggaaaggc ctggggtccg
     1261 cggaccgaga cggcggcacc ccgggcaggg gtgccgccgt ccgcgggact tggtcagctg
     1321 tatgtcaacg cggtgtcgtc gatgacgaac gacgtctgca ggtcggcgtc ctcggtggcg
     1381 gtgaaccgga tcttcaccgt ctgcccgatg taggagttca tgttgatcga cttcagcgcg
     1441 tacgcggtgt tctcgtccgc gttggagtag gtgacggatc cgagcacggc gcccgaggtg
     1501 ctgaggatct gtaccttgag cgtgtcgtag acggtcgttc cggtctccgc ggagtcgatc
     1561 cgcaggtaga acgacagcgt tgacgacgtc gcgccggacg ggatgctgac ggtctggtcg
     1621 acggtgtcgg tgtgcgtcgc gccgtagccg cacagccagg ccttccacga gccggtccgc
     1681 gccgggtagg tggcgtcggt ggtgatcacg ccgctgctgg cggtccagct ggtgttgccc
     1741 gactcgaagc cggggttggc gaggacctgg ccggagccgc cgccgagcga ggcgatcgtc
     1801 gccgccgcgt cgaccaggcc cgcgccgcac ccgccggagc acgatccggg cagtggccgc
     1861 gagttggcct tcagggaact ctccacgctc gccggggaca tcgacttctc gcccagcatc
     1921 agcgcgacca gcccggccac gtgcggcgtc gccatgctcg tgccctggta gtactcgtag
     1981 gtcgacccgc cgggcgtggt ggtgcccgcg ttcatcgcgg acaggacacc gttggtggcc
     2041 gtcgcggtct cgccgccggg ggcggagacg tcgatcaccg cgccgtagtt cgagtacgac
     2101 gcccggttgc cctggcggtc ggtcgcggcg acggtgatca cgccgccgca gttggcgggc
     2161 cgcgtgttgg ccgcgtcgat gttgctgttg ccggcagcga cgatgaccga cgagccgcgg
     2221 ccgaccgcgg agttgatcgc gttctggtag gtcgtgccgc acgcgccgcc gccaccgaga
     2281 ctcatgttga tgaccttggc cgggttcgcg ttggccggga cgcccgggac ggagccgccg
     2341 gaggcccaga tgatcgcgtc ggcgatgtcg gacagcgcgc cgccgcagaa ccccaggacc
     2401 ctcgcgtgca ggaccttggc gttcggggcg acacccgcga cacctgaccc gttgccggtc
     2461 accgcggcga tggtgcccgc gacgtgcgtg ccgtgccagg aggagtcgct cgggttgctg
     2521 cccacgccgc agtcaccggc gttgggcacc cagtcgccct ggtcctgcgg gttggagtcg
     2581 cggccattgc cgtcgcgggc cgcgctcgac gagctgatga agtcgtagcc gggcagcgtg
     2641 ttcgcgtcaa ggtcggggtg gaacgtccga ccggtgtcga tcacggcgac gaccgacccc
     2701 gcgccgttcg cggtgtccca cgccgtcggc aggcgcatgc ccgcggtgga ctcccagtag
     2761 tgccactggt cgccgtagta ggtgtcgttc ggcgagtacg tcggatagaa ccgcgagtcc
     2821 ggttcgaccg cctcgacatc gggctgcgcg gccagcgtgc gcaggaagcc ccgcgcctgg
     2881 gccgcgtcga gcccgcggtc ggtccgcacc acctgactgc cgagcgcggt gtcccggagc
     2941 tcctcgacgg cgacgccgtg cttggtgccc gccgcacgct gcgacgcgac tcgcttggcc
     3001 ttgtcccgac gcgcctgcga ctgtggcttg taggtcacga tgtactgatc gacaatatcg
     3061 ccgtcggcga cgttctcgac atatgcgcgg cccggttgtt cggcattggc caccggcgcg
     3121 gcgacaattg cgacactcga cgcggcgagc gcgataatga tattgagcgc gattcttcta
     3181 cccacgactg cgcaccttcc tgaactgcgg cgctttctgt gggtcgtgag tctgcgtgcg
     3241 gaaatacctt aaggaaaccc ttaactctta acagtcgaat tgcgcgactc aggcctgcct
     3301 tgatcgaacg actcgcgaca tatggatttc cacgatcgtt tcgaacgaaa tcatgccgcc
     3361 gaaggaatca tggtccggcg agaccaggac cgcccggcgg ttcgggatct gcgtggccgt
     3421 cggaggtgag ccaggcgacc agttccccga gcttgaggcg gtctgcgggt agcactagac
     3481 gcggtcgtcc gccatcaggc ggttcagcag ccgtggtacg tccaagacgg tgtcgacggg
     3541 agcccaggtc ccacccgcgg cgcgggccaa ctgccgggct gcctcgacgt cgtcgcccgg
     3601 ggccaggata atcagctcct gcaaccccaa cgccgcggct acggccccgg actcctccgt
     3661 tgcccgacag tccgacagga gcagggtgac cttgcggcgc gcgccggctt tgcccagctg
     3721 cgagtgagcc tcgtcgagcg cggcggatag cgacgtcgtc ccgtgtccgc gcaacgacaa
     3781 gatcgacgcc accgtctcac tcgccggtct ccgctcgttc atcggcttga gcacacccac
     3841 gtgggccgcg aaggtgatca ccgcgtgctc tcgaggcgtc aggctggcgc aggcggctgc
     3901 cgccaccgcg gccgtcgtca gccgggcgcc gttcatcgag cctgacctgt cgatcaccag
     3961 acacagcgcg agctctggcc tcgcccagtc gctgacgagg agctcgtcga gcctcgggct
     4021 gcgaccttcc gctcgcgcgc cgagcacccg gtccatggat cggtcgaggt cgatgtcgcc
     4081 tccgtccacg gcacggcgga tgcgcctcct cgcgataccc gaggacttcg gcaggccgcg
     4141 ccgcgtctgt tccaggatca gtcgaggggc cagtcggcgc acctggctgc gcagagcgtc
     4201 gtcggtcgcc agcgacatct cgacgatcag cgtgagcgtc tcgtcggcgt gttcggccag
     4261 ggccgagttc aaggcgtcct ggtccaggac gcccacctcc ggggacaact cggcgaagcg
     4321 cgggttgcgc gccaggtccg agcggccggt cgtgcgctcg ccgcgggggc ggtcacgcgc
     4381 atgtgggctg tgcgtcggct gaggtgagcc cgtcggggtc gacgggctca gcgttccctc
     4441 gggcgacgcc tcggggtcgg ggtctgccgc gttctgggca ggctcgggac cgaagacgcg
     4501 ttcatacatc tccaacacga cgtcctcagg gctccggcct gcggagtcgt ggagccggat
     4561 ccgaccgctg agtgagacct tggccgcggc cagacccacg tgccaatcgt tcaccgccac
     4621 cccgcgcagc cttgccagct cgaccgcgat gcgggcgatg tcgatggcgc cccgcaccga
     4681 cgatccccat tcgagagcgt cgtgctcacg agaggcacgc acgagtgcga ccactttgtt
     4741 ccgccaagac gcgtcgatgc cgggggcgtg cagcgagacg atgtcgcgct ccgcctcggc
     4801 cgactggtat cccatggaaa tgcggcatgt ccggtcgtac agcgcgctgg agacgcgtgc
     4861 cgtgcccacc gtgtcgaagg gattcatggc cgcgacgagc ccgaagcccg cagctgcgac
     4921 gatgcgtccc agccggggta cggcgatctc cttctcggac atcgcggcca ggagaacgtt
     4981 cagggtctcc tcgggaatgc gattgagctc ttcgacgtag aggaggcctc ctcttctcat
     5041 ggcgtcgagc aggggtccgt cctcgaagat ctccgcgacg tagccgcggt ccaggaccag
     5101 tgccgggtcg aaatggccga tgaggcgggc cggagtgagt tccgagttgc cctccacgag
     5161 gacgaacggg atctcacgtt cgcgagcgac ctgccgcagc agggtggact tgccggtgcc
     5221 gggaggccct tcgagcagca cgtgtgtgcc gctgagcagc gcggcttcga ggatctgggc
     5281 ctcgtcggtc cgggcgaccg cgtccatggg tgcggcagcg cctgccgcac ccgtcgacag
     5341 tgacggctgg ttggccactc agtgctcgtg gacgacgacg ccgcgcacgt tcttgcccgc
     5401 gaggaggtcg tcgtagccct cgttgacctg gtcgaggcgg tatgtgcggg tgatgatctc
     5461 gtccagcttg aggtcgccgc tctggtacag gccgaggatc ttggggatgt ccacggtggg
     5521 gttgcagtcg ccgaagaggc tgcccttgat ggtcttgcgg aacagagtca agatcgagcc
     5581 tggcagctga attgtctgct cgtcgagctt gttcagtccg gtcacgacca cggtgccgcc
     5641 cttgccgatg gcgttgaacc cgctggtgac gatctcggcg gtcaacaggt cgggcgtcag
     5701 gatcgcggaa tcggcccctt gtccgcgggt cagctcaacg atcttgtcct gtgcctgcgc
     5761 ggcgtcggcg aacgcgtgcg tggcgccgag ctccatcgcc ttttcccgct tgttctccag
     5821 ggggtcgatg gcgacgatgt tcttggcgcc cgcaaggcgg gcgccctgca cggcgttgat
     5881 gccgatgccg ccgatgccgt agacggcgac cgtgtcaccg ggctggacct tggcggtgtt
     5941 gaccgcggaa ccccatccgg tcggcacgcc gcagccgacg agcacagcct tctccagcgg
     6001 gaggtcgtca tcgaccttga cgcaggagtt ctggtggatc acgccccact ggctgaacgt
     6061 gcccagcatg cacatggcgc cgtactggcc gcgcgggcca gagatcggga agcgatcgcc
     6121 cggcaggtag ccctcgagga tcgtcgcgcc catgtcgcag atcgactgct ggccgttcgc
     6181 gcaatagcga caggtgccgc agttggggat gaacgagcag acaacgtggt ccccgacctt
     6241 tacgcgggtg acaccggggc cgacttcctc gatgattccg gcgccctcgt ggcccagcac
     6301 catggggagc cgcgcttgga tgtcgccgtg ggcgacgtgc acatcggaat ggcacagccc
     6361 agcgtgcgtg tagcgaatca gcacctcccc ggcctgggga ccgtcaaggt cgagttcctc
     6421 gatctcgatc ggcttcccgg gctcgtagac gaccgcggcg atggtcttca cgggtcctcc
     6481 taagttgtga tggggaccac atcatcgcaa gattgaggaa caaactctgt tcaaagtctg
     6541 gacaagtcga gttcacggcg cgggtcacac tgtgtgacgg cgataacagc ccgaagggag
     6601 ccagccctat ggactcgcat cggcccgctg ggccagcggg cgcaggcgtc cgcgaagaca
     6661 tcgcgctgtc ctggcagcgc gcgaccatgt ccggtctcga cccgtccgcc gagttcgaca
     6721 cggcggccca cgacgtcgac ctgcgcagtt cgctgctggt gggcgcgatg ccggttctcg
     6781 aggagctgga ggagaacctg cgggggagca actactgcac gctgctggtg gaccgcgact
     6841 gtcgagtcgt ccgtcggtgg ttcgacgacc cgcgcctcga atgtggcttc gacgctctca
     6901 atgtcacgct cggtgcctgc ctcctcgagg agaacatcgg caccaacgcg ctgggcaccg
     6961 tcatggtcac ccggcagggc atcagcatca gcgggcacga gcacttcgcg aaatcccttc
     7021 agggtttcag ctgctacggc cacccgatca ggcatccgct caccaagcgc atcgaaggcg
     7081 tcctcgacat cacggcgatt gtcgaccagg ccagcccgct cttgccgccg ctgatctccc
     7141 gggcggtcca cgacatcgag cagcgtctcc tggacggcag tcgcacctcg gagaaaagcc
     7201 tgcttttggc gttccaaggt gcggcaggcc tgcggcgtcg agcgatcctg gccattggcg
     7261 aggacatcgt gctgtcgaac cacgccgcca acgatttgct cagtcctggc gacatcgcgc
     7321 tgcttcgcgc ccttgccgaa gagtcgccgc gcgaggaaca gacagtggag ctcacgctgg
     7381 aggccggtgt cgccgtcagc atcgaaatct cgcgcgtggg tggcgcgtgt cgtggcgcgc
     7441 tcttgcgggt ggaccgcgtg agcggcgatc gggcacgggc ggtactgcac cgatcccaga
     7501 cgaaactcaa ggaggtcggc gctccccgca tggtcaccgg cgtaccaggt accggacgtt
     7561 ccacgcgagc aagagagttg gccgccgagc atcccccgtt gaaggtcatg cgaccagcgg
     7621 ccgcgctcct cgaaggcggc gcggagtggg cctgcgagtt cgaggaggcg atgcggcgtc
     7681 cgaaggggac ggtgtgcgta gacggaatcg acctgttgtc cagcgagttg ctcgacctcg
     7741 tcgtcgactg ggtcgatcgt gacccgcgcc ctgacctgat cctcacggca ggtccgatcg
     7801 cgggcctcac cggccgcgcg gccgcgttgg ccgggatggc gatgatccgc gaggaactcg
     7861 tccccctcgc cacccggcgc aaagacatcc ccgggctggc cgcggcgatg ttgagcgagg
     7921 tggcgccgaa tcgggcgctg cacctggctc ccagcctcat ccaggccctc gccgcccagt
     7981 cctggccggg gaacctctgg gagctcaagg ccgtgatcag ctacctggca cagcaacggt
     8041 ccgtgggcgc cctcaccgtg cgagatcttc ccgagcaata ccgttcgacg tcgctggacc
     8101 acccgctgac ggcgttggat caagccgagc gggacgtgat cgtcacggcc ctcaaagccg
     8161 ccgacggcaa caaagtccgc gcggccgaag aactgggcat ttcccgcacg accctgtatg
     8221 cgcgcatgag aaccctgcgc atcacgacgt actgacgtcc cgggactgtc cagagactga
     8281 acaccggcgc tcgcggtcgg gagtgatcct ggtcgcacgg acctcgcagg aggcggggtc
     8341 gccgcacccg gaggcatcca tgacgtttgt tcttcccgac ctacacgctt cggtcatccc
     8401 ggccgtacgc gacttcctgg gtaccccgaa gcagttgttc atcggcggag aatgggtcga
     8461 cgcggcgagc ggcgaaacgt tcgacacggt cgaccccgcc acgggacgag tcctcacgac
     8521 cgccgccctc ggcggcgcgg aggacatcga ccgggccgtc aaggccgcgc gcatggcctt
     8581 cgatggcgaa tggagcctct ggacaccggc ccagcgccag cgtctgctgt tccagatcag
     8641 tgaggcgatc cgggatcggg ccgaggagtt cgcccagctc gaatcgctcg acaacggaaa
     8701 gtcggctgcc gtcgcccaag ccgtcgacgt gctgtgggtg gccgagttgt tcgcgtacta
     8761 cgcgggctgg gcgaccaaga tcgagggtcg aacgatcccg gtctccgtcc cttgggcgcc
     8821 cggcgcccag tggcacgcct acacgctgcg ccagcccgtg ggtgtctgcg gcggcatcgt
     8881 gccttggaac ttcccgctgc tcatggccgc cttcaaggtg cccgccgcgc tggcgtgcgg
     8941 caacacgacg gtgctcaagc ccgccgagca gaccccgctg tccgcgctgc tgttggccca
     9001 ggtgatggcc gacgcgggtc tgcccgacgg agtgttcaac gtggtcaccg gctacggcga
     9061 cgcaggcgcc tcgctcgccg cgcacatgga cgtcgacaag atcgccttca ccgggtcgac
     9121 cgaggtgggc aagctcgtcg tcgacgccgc tcgcggcaac ctcaagaagg tctcgctgga
     9181 actcgggggc aagagccccc aggtggtcta cgccgacgcc gacctcaatg tcgcggtccc
     9241 gggaacagcg agcggtttcc tgttcaacca cggccaagcc tgcacctcgg gcacccgcct
     9301 cctcgtggag gactcgatct tcgaggagtt cacacaggcc gtcgccgagg tggcaggcgc
     9361 gagcaagctc gggccaggcc tggacccgac ctcggaggtg ggaccgctga tcgacggcac
     9421 ccaattggcg cgcgtgaccg gcttcatcga ggcaggcctc aatgacggag cccgtgcact
     9481 caccggcggt ggccgccacg gcgacgaggg cttctacgtg gagccgaccg tgctggtgga
     9541 cgtccaggac tccttcagcg tctaccgcga ggagatcttc gggccggtcg tggtcgccac
     9601 ccccttcagc gcgcgggagg gagtccgtcg gcaggcgaac gacacgccgt tcggtctcgc
     9661 cgcgagtgtc tggacccgcg acgtcgccaa ggcccaccgc accgctcgcg agatcaacgc
     9721 gggcaccgtg tggatcaact gccacaacgc cttcgacgcc gccatgccct tcggcggctt
     9781 caaggaatcc gggtggggcc gcgaactcgg cgaaggcgcg atcgacctct acacccagaa
     9841 caagtcagtc aacgtcgcac tctgactcac cgcaggaagg gaacaccatg aacccggctg
     9901 acatgaaccc accgcaggac accgaggacg caggcgagga actcgtcgcg gaagacctca
     9961 tcgaggaggt ctcgatcgac ggcatgtgtg gcgtttactg atgccggacc tcctgctcga
    10021 cccgatgctg gggcaggcgt ggacactgtc cccgtcggtg gcgctgcgtc ccgaaccctt
    10081 cggtgcgctg gcctaccact tcggcaaccg caagctcacc ttcctcaagc gcccgcggtt
    10141 ggtgtcagtg gtccgggcgc tcggtgagcg cccggacgtg cggtcggccc tgatagccgc
    10201 aggcgttccc gaggcccagc acgcggcgta cgtcgcggcg ctgcgcgctc tggccgccac
    10261 cgacatgatt cgcccgcgcg ccgaggaggt gaccacatga ccgcggtgcg cgaacgtccc
    10321 ttgggcggct ccctcatcga tcagttcgag ctcggactgg acgcgccgat ctgcctgacg
    10381 tgggagctca cctacgcgtg caacctcgcg tgcgcacact gcctctcgtc atcgggcaag
    10441 cgcgacccgc gtgagctgag taccgagcag tgcgaggcgg tgatcgacga actgcggcgg
    10501 atgcaggtgt tctacgtcaa catcggcggt ggcgagccca cgatccggcc ggatttctgg
    10561 cacctgctgg agtacgccgt cgaccaccag gtcggcgtga agttctccac caacggcgtg
    10621 cggatcactc ccgagcgagc gcggttcctg gcgtcgaccg actacatcga cgttcagatc
    10681 tccctggacg gcgcgaccgc ggaagtcaac gactacgtgc gggggccagg gtcgtacgac
    10741 accgcgctca ccgcgctgcg gaacctccac gacgcgggtt tcaaagacgc gaagatctcg
    10801 gtcgtctgca cccgcgagaa catcgggcag ctcgacgagt tcaaggccct ggcggaccgg
    10861 ttcggcgcga cgctgcgcct gacgcggctg cgcccctcgg ggcgcggggc ggatgtgtgg
    10921 gacgagctgc atccccggcc cgagcagcag aaggtgctct acgactggct gatggcgcac
    10981 ggcgaggacg tcctgaccgg tgactcgttc ttccacctcg ccgcgttcgg cgaaagcctg
    11041 ccgggcctga acctgtgcgg cgcgggccgc gtggtgtgct tgatcgaccc gatcggtgac
    11101 gtgtatgcgt gcccgttcgc gatccacgcc aatttcctgg cgggcaacct cctggccgac
    11161 ggtggattcc agcgggtgtg gcagacctcc gagctgttca ccgagctgcg ctcgccgcag
    11221 accggtggcg cgtgcgcgaa gtgcacgttc ttcgactcgt gtcgcggtgg ttgcatggct
    11281 gccaagttct tcaccgggct gccgctcgac gggcccgatc ccgaatgcgt gcggggctat
    11341 ggcgaaaccg cgctcgccgg ggcgcgaacc attcccgccg cgagccagga ccactccaag
    11401 accggtccga cgcgcaacca gccggtgatg ctcaaactga tgacccggcc tcccgtgtcc
    11461 gcgtgctcgg agagcccgct cgccgactac tcgcccgaga acggctgctg ctcgtgaagc
    11521 tcgagaaccc ctggaaacag aacccctggt tcgagtccgt cgccgtcgcc caacgacgtg
    11581 cacggaagaa gttgcccgcc ccggtgtacg gcgccctcgt ggcgggctcc gagcgtggcc
    11641 agacgatgag cgacaacgaa gcagcgttcc gcgaactcgg gttcgctccg cacgtcgctg
    11701 gtcagtgggc agagcgatcg atggccacaa cggtgctggg ccaggagatc tcgctgccgg
    11761 tcctcatcag cccgaccggc gtccaggcgg tgcaccccga tggcgaggtg gccgtcgccc
    11821 gcgcggccgc cgcgcgggga acggtgatgg ggttgtcgaa tttcgcctcc aagtccgtcg
    11881 aggaggtcac ggccaccgga gcggcgacgt tcttccagat gtactggacc ggcgaccgcg
    11941 acgtagtggt ccagcggatg cggcgcgcgc acgcggccgg tgtggtgggc ctgatcgcca
    12001 ccctcgactg gtcgttctcg atgggccgcg actgggggag tcccgagatc ccggagaagg
    12061 tcgacctcaa gacgatggtc cgaatggcgc cgaaagtcct caccaaaccg cgctggctct
    12121 accagttcgg gcgcaccggc catatccccg acctcaccgc acccaacctg gcacctctcg
    12181 gtggagaggc gccgacgttc ttcacggcgt actacgaatg gatgaccacc ccgccgccct
    12241 cctgggagga cgtcgcgtgg atgcgcgagc agtgggccca gatcagtggg acgccgttcc
    12301 tgctcaaggg ggtctgtcgc gtcgacgacg ccatccgcgc ccgcgacgtg ggggtctccg
    12361 gcctttctgt ctccaaccac ggcggcaaca acctcgacgg cacgcctgcc gcgatccgga
    12421 tggtcaagcc gatcgccgac gctgtcggtc acgacctcga tgtcgtgatg gacggcggca
    12481 ttcgccgcgg ctccgacgtc gtcaaagctg tcgctctcgg cgcccgggcc gtgatgatcg
    12541 gccgcgccta cctgtggggt ctcgcggcca acggtcaagc gggcgttgag aacgtcctcg
    12601 acgtattgcg cggcggcatc gactcggcgc tcatgggcct gggtgtcgcc tcggtcgacg
    12661 agctgacccc cgagcacgtg ctggtgcccg cgggattcca ccgcactctc ggcgtacgga
    12721 ctccggaggt cgcaggcgga tgaccggtca gatcgcggac gccacctggc ccgagctggc
    12781 ccaggtcgat ctcgtgctgg tgccggtcgg gtcgatcgag cagcacggac cccacctgcc
    12841 gctcgacacg gacaccgcga tcgcgaccgg cgtggcgcgg ggtgtggccg cggctctgga
    12901 gggggcctgg gtggcgcctg cggtcgtcta cggctccagc ggcgagcacc aggcgttccc
    12961 gggcaccagc tccgtcggca ccgatgccct gcgtctcatg ctggtcgagc tggtccggtc
    13021 catgcggacg tggacgcgac gcgtgctgtt cgtcaacgcc cacggtggca acctgcgggc
    13081 cctggacggc gccgtccgcc aactggtgga cgaaggccac gatgtcacct gggcgccgtg
    13141 cgcgacggag gaggtcgatc tgcatgccgg tcgcaccgag acctcgctga tgctgcacct
    13201 gcgaccggac tcggtgcgcc tcgaccggtc cgaggcggga aacactcagc ccctgcaaga
    13261 gatcctgcca accctcatca cgcgcggcgt gcgtgccgtc tccgccaacg gggtgctggg
    13321 cgatccgact ggcgccacgg cggaggaagg ctcccggctc ctcgcgcgga tggtgcgcga
    13381 cgtcttggcg cgcgcgacat gagccgggtg gcgatcgtca ccggagcggc ccgcgggatc
    13441 ggagcggcga ccgtcgcgcg actggtcgcc gacggctatc gggtcacggc agtggactcc
    13501 tgcgagggcg ccccgctggc cactcgcgct gacctcgacg ccgtggccgc cgcccacgga
    13561 gacaccgtgc tcgcccacgt cgcggacgtc cgcgaccgcg ctgccctggc cgaggccgtc
    13621 acgcacacga tcgaccgctg gcaccggctc gacgtggccg tcgcctccgc cgcggtcatc
    13681 cgtggcggcg caccgctgtg ggagacggcc gaggcagacc tggacctgct gtgggacgtg
    13741 gacgccaagg gtgtctggaa caccgccgcc gtcaccgtgc ccgcgatgct ggcaggcgcc
    13801 gacccgtccg gttgccggtt cgtggccgtc gcgtcggcgg caggcaccca cggcatgttc
    13861 cacctggccg cctacaacgc cgtcaagcac gcggtggtcg gcatcgtgaa gggcctggcc
    13921 gccgacttgg cggccaccgg cgtcacggcc tgcgccgtct cgcccggctc caccgacacg
    13981 cccatgctgc aggcgaccgc cgacctctac ggcgtcacgg ccgcggaact cgcggggcac
    14041 cagctgatcc ggcgaccgat cgaccccgcc gagatcgccg ccacgatcgc cttctgcgcc
    14101 tcgctggcgg gtcgggtcgt gaacggcggt gtcgtgcacg ccgacggcgg cttcgcgtca
    14161 tgaccacgtc agccgcggcc cggctcccgg acgggttcga ggtccgcatc cgcgacgacg
    14221 tcacacggtt gggcgatggt cgcgtgctgg tcgggggctc gccgttgcgc gccatccggc
    14281 tcagcagcgg cgcgatgggg ctgatcgacc gagaccgggt tcgggtggtc gacccccgga
    14341 gcggcgccct cgcgcgacga ctcctcgacg ccaacgtcgc cgaccccgtc ctgcttgcga
    14401 tggtcccggc cgactcgatg acggtggtga tcccggtgcg ggaccggccc gaacagttgg
    14461 accgtgccct ggcagcactc cacccgcttc ccacagtcgt tgtcgacgat ggctcgaaag
    14521 ccccggaggc cgtcgccacg gtggcggcgc gccatggtgc gcgacttgtc ccacttcagg
    14581 tcaacctggg accggcgggc gcgcggaacg ccgggctcgc gaccgtccag acgccgtacg
    14641 tcgcgttcgt cgactccgac gttgacgtca cggctgacgc cctgatgcgc ctcgcacagc
    14701 acttcgccga tccatgcgtc gccctggtgg ggcctcgggt cgtcagcgtc gcgcgctccg
    14761 cgcggccacg atggcacgag cggtacgacg agtccgcatc ctcgctcgac ctgggagttc
    14821 gcgcctgctc cgtgcgcccc ggcgccgcgg tcgcctggct gcccagcgcg tgcgtcgtcg
    14881 gtcgcgttga tctgctggcc gacgggttcg acggcgacct gcgcatcggg gaagacgtcg
    14941 acctggtgtg gcggctcgtc gccgcgggcc acacggtccg ttacgacccc tccgtgcttg
    15001 ctcgacacga cacgcgaccg accctgaccg ggtggctggg acgcaagttc gcctacggca
    15061 ccggtggtgc cgtgctcgca cagcggcacg gcgctcacac ggcggtggcg caattgtcgc
    15121 ctgcgatggc gctggcggcc ggtgcgctgc tcgtccgccg acgctggtcc gtgccgatcg
    15181 ccgccgtctg ccttgcgcgc ggcgccaggt ccttggccac cagcctcccc gatccggtga
    15241 tcacgcctcg actcgtgggc cagatctccg cgcggggatt gggctgggca ctgcgccagg
    15301 agtcgtcgct gctgctgcgc cactggtggc cggcagcact ggctgccgcc gtgtggagcc
    15361 ggtcgatacg ccgcgccatc gtgacggcga ccgtcgtgga cctcatcgcg gccaccctgg
    15421 aaggagttcg cctgcgaccc atcgctgcct tcttcggacg tcggatcgat gatctggcct
    15481 atggcggtgg tctctggtgg ggctctatcc gggcccgttc tccccgggca ctcgcggtgc
    15541 ggttggtccg ctcgatcggg atctcaacgc cgcgcttgga gccgacggcg atctccaccg
    15601 ccgggccgga gaggatcagt ccggccaggt cggcggggtt gccggtggtg cactgcaggg
    15661 cgatcaggcc gcccatgctg tggccgtaaa ggcgcgtgct cggcgatgcc gtgcgagatc
    15721 accacgacgg ccttggcgtc gttggcgtcc gggccggtcc agccctgcca gtggatggtg
    15781 ccgcccgcgg taccggtgaa ccgaccctcc acatgctccg tcatcggccc tccacggtgg
    15841 atagctcgag cacctcgcgc aggccctcgt ccagacattc catcatcacg tccggatcct
    15901 ggaccgcgct accgtccata ttgatgccga agcagcagac cccggcatgc gagaccatcg
    15961 ccgccatcac cgcgacgcct ggcagcgggc cgaacgggaa cagccgctcc accttcgctc
    16021 cggccatgta caccgggtgc ggtacgcccg ggatgttgga cgcggacagg tcggccgcgc
    16081 tgccgatctg gtcgtagacg aacccgccga ccgccgacgg aatccggttc agcaccggcg
    16141 cgaccatcga gaaggtgtcc aatgcgggtt cggtgcgcag cgagagcacg gtgccgcgga
    16201 tggtggcgat ccgctccgcc gggtcctcca caccgaccgg cgcggcgaag aacgcaccgg
    16261 cgaacttgtt gccgcccaac ggatcgtcgg ccttgcgcag gctgaccggc atcgccatcg
    16321 gcagttcgtc gatcgacgtg ccgtgccggt cgtggtagcg ccgcaacccg ccgagcagag
    16381 cggcgacata ggcgtcgttg agcgatccac cggccgcctt cgccgccgaa cgcagctccg
    16441 ccagcccgca ctccagcaca ccgaacttcc aggatgtgcc ggaccggttg gtcagcagcg
    16501 gggacggcgc cgcgggtggt ggcgagaggg tgcggcgcat cgacccggcg aatctgaccg
    16561 catcgctgag cacctccgac gggtgcgtcg cggcgtacac gcccatcgac agcgcgcgcc
    16621 acagcacgcc gggcacccgc cgggcctgct cggtcagctc gaccgttgcc agcccggagg
    16681 cgtccatggt ttccctggcg gtcaacggat ccgcgggcag cggcttgttc ggggtgtgct
    16741 cgcgggtgcg gctctgcacc atggtcatca gctggatgcc gcccatcccg tcggtgagtg
    16801 agtggtgcag cttcagcacg taggcggcct tttcgccgtc gaatccctcg accagggtgc
    16861 cctcccaaag cgggcgggtg cggtcgaacg gcgtcatcgc cagtgactgg gccagttcca
    16921 gcagtgcggc caggccgccc ggggcgggca ggcgggtacg ccggacgtgg tagtcgagct
    16981 ggaagttctc gtccggcacc caggcgggcg ggccgaccgg cagggcgggt tcgagcaccc
    17041 gctggcggca gcgcgcgatc agcaggctgg cccaatcgtg cgcggcgaag aaccggtccc
    17101 agtccgggac ctggtcgtag atcagcaccg aggtgatcgt ggaggacagc cgcggatgcc
    17161 gttccgagcg ccacaacagc gcctcgatct cgttcatccg gtcgctggtg ttccacccgg
    17221 aggcggcggc ccaggcctcc tcgttggtcg aaagcggtcc ggtcaccact cctccccgtg
    17281 cacctcgtgg ccgggcgcct tgcgcagcag gccacggtag gtgtcctcga ccggctgccc
    17341 gtggttgatc accgcatcca tctcgcgggc gatcggcatg tcgatcccgc gctcggcggc
    17401 cagcttcatc acggtggcga cggtcttgac gccctcggcg acctggttca tctcctgcgc
    17461 gatctgatcc agcgacttcc cttgcgccag accgactcca accgtcctgt tgcgactgag
    17521 cgggctggtg caggtggcga tcaggtcgcc catgccggac agccccgcga acgtgtcggc
    17581 ctgcccgccg agtgcgacgc cgaggcgggt cagttcacgc agcgcacggg tgatcaccat
    17641 cgccctggtg ttgtcccctg cctccaggcc gtcgccgatg ccgaccgcga tggcgaacac
    17701 gttcttcagc gacccggcgt actccacgcc gatcacgtca gtggtggtgt agacccggaa
    17761 acgcgtggta cggaacagat cctgcagcat cgaggcctgc tgctgctccg gcatggcgat
    17821 ggtcgccgcg gccgcgtaac cctgcaccac ctcgcgcgcg atgttcggcc cggccagcac
    17881 accggccgga tgccccggca ggatctcgtt gacgatctcg gtcatccgct tccgggtgcc
    17941 ctgctccagg cccttcacca ggctcaccac cggtacccac ggccgcacgt gctcggcgac
    18001 ctgctccagc accgaacggc tgtgctgcga cgggactccg agcacgatca cgtccgcctt
    18061 gcctaccgcc tcggccagct ccgcggtcgc gcggagcgcc tcggtgagcg gcaagtcgtt
    18121 gagatagcgg gaattgcggt ggttgtcatt gatgtccgcg accgtcgccg ggtcacgcgc
    18181 ccacaggact gtgtcggtgt ttcgcgccgc cagcgaagcg acggtcgtac cccacgaccc
    18241 gccgccgagc accgcgacac tgggcatcct gccagccatc aatctccctc tcgatccggc
    18301 cacttctcaa gcgtgtcaac gaacttctgc cgcacctcgg cggtgatccg gtccaggtcc
    18361 tcgaccttcc agtcatcggt cgggatcggg tcgagcacgc acacttcgac ggtgccaggg
    18421 tgcaccagtt tggaccggcg ccacatcagc tcgccggtgt tgcgcaacac gatgggcacc
    18481 atcggcacgc ccgcctggat agcgatgtgg aacgcgccct ttttgaaccg cgccagcgcc
    18541 ggagtcggcg cccgggcgcc ctccgggaag atcacgatcg acgtgcccga gcggatccgc
    18601 tcaccgagct tgtccagctc cgcgatggcc ttctccgggt tggagcgatc gatgaacgcc
    18661 gggtcgagca gcaatccgcc gatcagcgtc cgtggatcgt actgcgcctc cttcttgccg
    18721 accgcggtga aatccttgcg cagcaaggaa cccatcacaa ttgaatcaag cgagctctgg
    18781 tggttgccga tgaagatcga cggccgcgcc cgccacaggt tgtgctcgcc gatcacgttc
    18841 agcttcaccc cggccagcgc cagcgccgag tcgcatgcca gcgggatgcc ggtgttgatg
    18901 ccgaacttgc ggtcacggtt gaggatgccg agcgcggcgc cgaccccgag gccgacgttg
    18961 agcccggcca gcgcgccgac cgtgcccagc accgaacgca gccccgcctt cctcggctcg
    19021 cgaagcctca gcaccggcca cttctgcagc gccgccgcgt ccagcaagcc gcggtgcggg
    19081 ttgatcgcgt acggcaggcc tgccgaggcg agaaacgcca catcctcgtc gccgttggcg
    19141 taggcgtggc tggcggcgag gtcgattccc ttgtcccgcg cgaactgccg caccgcccgc
    19201 gccttcggct caccccacag catcttcccg gccaggcgcc cggtgaggat gccgtcctcg
    19261 gtctccaatt cggtgcacaa cacgtgagtc acgcccagat cagcggcgat cggctcgatc
    19321 tggaaccggg tcgccgccga agcgaaggcc accgtgtgcc ccatcttcag gtgcgcccgg
    19381 accaggtcgc gggcctgcgg acggatggtg cccgcggtct tctgcacgaa cagccgctcg
    19441 cccagttcga ccagttcgtc ctcaccgcgt ccgcgcaggc ccccgacgct cacatcaccg
    19501 atcttggtcg ggttgccgcc aagcaggttg ccgtccagcg ccgcgaccat tgtcctggtg
    19561 aactcgccga cgccgatgtc acggctgcgc agccggtcgt tccagaacgc actggccgtg
    19621 tagcccgcca cgactgtgcc gtcgaggtcg aagaacgctc cgatccgcgg accacccggt
    19681 ccactatgga tggacgcgac cacgtcttca ggtgtgatca tcggctgctc gccctggcaa
    19741 gctgcgcgac gactcccgca cggcgggccg cctcccggat ctcggccagg aagtccttgc
    19801 ggcgcttggc cagatgcggc tccgccgagt cgatcagatc acggtgtcgg gccaggcgca
    19861 gcgccgtccg gaacagctcc agcgtcaccg actcgtcact tgccactcgc cgctgcagcg
    19921 cccactggcg gcccactcgc aggcactcgt cgaggaagcg cttctcctcg aactcgtcgt
    19981 cccacgcggc cagccggtcg gccaccacgt ggtaggcgtc caggtacgga cgcagcacca
    20041 ggtgcgcgac atgtggccga gcccgttcca gccgcagccg cagatccgcc gggtcgaact
    20101 cgaccttggc gtcgccgaag agggccagtt cctcgcgcag ttcgtgaccg aactcgtcgc
    20161 ggccggagaa gaagaactcg aacttgagca gctcgcgcag ccgcagggcc tccccgcgcg
    20221 cggtccgcgc cacctcgccg gtgccctcga ccgctgaatc ctcggccgcg gcgagcagcg
    20281 ccaactcgcc gatcgcccgc tcgaccagaa cgtggatcgc ggtgttgcgg tagaacgcgg
    20341 caaccagatg cctgtccggc gcgatgcccc acaccgtctc ggtgccgccc tcgtagcagg
    20401 tgagcacgcc ggaggaggtc atctcctgca gtgcgcggcg gatcgtcgaa cggtcggtca
    20461 agttcgccgc cccggccacc ggccaactgc ggctgctcaa atagtcggcc agtggagcga
    20521 ccgtcgccag cacctcgtcc agcgacaacg cgcggtccgc gcccagcatc gccaggctga
    20581 ccaccgcggt cggggtgacc ggtgtggccc ggttgatccg gtggcacacg tccagcgcga
    20641 cccgctcgat cacgtgcggc gccgccgcct cgtccgcccg cagttccgcc agccgctcgc
    20701 tgagcgagaa cggctcaccg aagtccaggt aggcgcggcc gaggcgctgg cgctgctcgc
    20761 gggcgaagtt gaccagccag cgcaggtcct cgggccgttt gctgccacca cgcgcctccg
    20821 tggtcatcat ggcgacctcg tgcagttggt cgtagacgat cgacaccggc acgaccagag
    20881 cctccgcggc gccgacggcg tccaccgcgt cagcgatgta gcgcaggatc ccgaacaccg
    20941 gcgggcgcag cttgccggtc cgggtgcggc cgccctcgat cgaccaagcc aggttcgccc
    21001 ggttgcgcac cagctgagcg atgtagttgc gcagcgccca ccggtacacc ggcatgtcgg
    21061 acgtgttgcg ccggatgaag atgacgcccg cccggctgat ccagttgccc accgggaaga
    21121 acttcaggtt cgcgccaccg aaggtgtatg cgggcgagat tccgcgggag ctgaccacct
    21181 ccgggaccat cgttccgtcg agataggagc ggtgggaaaa caggaacagc agcgaatgct
    21241 tgcggtccag ggcccgcagc cgactgaccg actcgtcatc gacatacaca tcgtgcgcgc
    21301 gcatcagcca ctgactgaac cggctccacg tgtcgcccgc cctgtcgccg tgggtcgcgg
    21361 acatctcgcg caggtacccc gcggcctccg cccggacatc gtcgggtttc ttgcccagcc
    21421 gctgcgccag gctcgccagg ctctcgtcgg cggccgtgtc gccgagcagg tcggccacct
    21481 cccgcgtgtc cgaaccgagc ggggtgaccg cctcctcgag tacgcccgcc cgctgcagca
    21541 gtcttcgcgc gtgcagccga tccagcgcac cgatcaaggc cgggtgcccc tcgcccatga
    21601 ccgcctccct ggttatgtga accagatcac agagggttca gtcttgttga gcacctcgtc
    21661 aaggtcaatg gagccgaacg gatcgccacc ctcggcgcca tcactcaaac tagacgcggc
    21721 cgacgacgcc gaaccgagcc gtcgacatcg ccatccggct acccgcagac caactacccc
    21781 acatcgccga aacggcggaa aggatcaacg aacctgatca aaacaccagc agcgcctgct
    21841 ccgacggcag accctgcttg tgtagatgct gtacgtgccc ccggtcagag tcgaactgac
    21901 actggacgga ttttgagtcc gctgcctctg ccggttgggc tacgggggcg tcggctgagc
    21961 actctacgcg atcagtcgga cggctcgcgc ggaggttggg gcgttggggt gatgtcgggg
    22021 gtgtcacgtc gccgggtacg tggtcaaggg gtacgcggcg gtaggcttca ggttccccgt
    22081 ttcctggcag caaggaggac cccggtgacc gagctggcta ccgaggccca agccgccgcg
    22141 ccccgtcggg tgctggtggc ggaggacgag gcgctcatcc ggcttgacct ggtcgagatg
    22201 ttgcgggaag aggggtacga ggtcgcgggt gaggccgccg acggcgaaga ggctgtgcgg
    22261 ctggccggtg aactcaagcc cgacctggtg atcctcgacg tcaagatgcc caagatggat
    22321 gggatcgacg cggcgacgac gatcgcgggg gagcggatcg cgccggtggt gatgttgacg
    22381 gcgttcagtc agcgggagct ggtggagcgg gcgcgggacg ctggggcgat ggcttatctg
    22441 gtcaagccgt tcgccaagca tgatctggtg cccgcgatcg agctcgcggt cagccggttc
    22501 tccgagttgc aggccctgga gaccgaggtc gccgggctca ccgagcggct ggagacgcgc
    22561 aaggtcatcg agcgggccaa ggggctgctg atgagcaagc aggggctgtc cgagcccgag
    22621 gcgttccggt ggatccagcg caccgcgatg gaccggcgta cgacgatgaa ggccgtcgcc
    22681 gaagccgtgg tggagaacat cacctaggcg ccgccctgcg agtccggcac gcgctatccg
    22741 cgc
//
