LOCUS       NZ_FHRS02000028        21187 bp    DNA     linear   CON 12-DEC-2024
DEFINITION  Streptococcus pneumoniae strain GPSC18 substr. ST1797
            ERS455146SCcontig000028, whole genome shotgun sequence.
ACCESSION   NZ_FHRS02000028
VERSION     NZ_FHRS02000028.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2016) WTSI, Wellcome Trust Sanger Institute, CB10
            1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900055005.2-RS_2024_12_12
            Annotation Date                   :: 12/12/2024 02:43:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,241
            CDSs (total)                      :: 2,189
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 73 of 180
            Pseudo Genes (incomplete)         :: 93 of 180
            Pseudo Genes (internal stop)      :: 57 of 180
            Pseudo Genes (multiple problems)  :: 36 of 180
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:31:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 21187
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FHRS02000028.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..21187
                     /collection_date="2012"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /isolation_source="BLOOD"
                     /mol_type="genomic DNA"
                     /note="contig: ERS455146SCcontig000028"
                     /organism="Streptococcus pneumoniae"
                     /serovar="7C"
                     /strain="GPSC18"
                     /sub_strain="ST1797"
                     /submitter_seqid="ERS455146SCcontig000028"
     region          1..21187
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /tool="antismash"
     cand_cluster    1..21187
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21187
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[8212:11203](-)"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(8213..11203)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     CDS             109..216
                     /gene="allorf_00109_00216"
                     /locus_tag="allorf_00109_00216"
                     /protein_id="allorf_00109_00216"
                     /tool="antismash"
                     /transl_table=1
                     /translation="MTISLSRSVRCETKKTPAMRVCVEGYTKKTPNAIQ"
     CDS_motif       109..165
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MTISLSRSVRCETKKTPAM"
                     /locus_tag="allorf_00109_00216_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       166..216
                     /prepeptide="core"
                     /locus_tag="allorf_00109_00216_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-6.50"
                     /molecular_weight="1771.0"
                     /monoisotopic_mass="1769.9"
                     /core_sequence="RVCVEGYTKKTPNAIQ"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MTISLSRSVRCETKKTPAM"
                     /leader_location="[108:165](+)"
                     /alternative_weights="1789.1"
     misc_feature    899..912
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.41"
                     /tool="antismash"
     gene            complement(931..1563)
                     /locus_tag="A1898_RS09785"
                     /old_locus_tag="SAMEA2521606_01976"
     CDS             complement(931..1563)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /locus_tag="A1898_RS09785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01976"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000571422.1"
                     /transl_table=11
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLMN
                     MIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIGQK
                     LSRSEQRLRQKQALEQVGLVYLDLDKRIFELSGGESQRVALAKIILKNPPFILADEPTA
                     SIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(1565..2332)
                     /locus_tag="A1898_RS09790"
                     /old_locus_tag="SAMEA2521606_01977"
     CDS             complement(1565..2332)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677041.1"
                     /locus_tag="A1898_RS09790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01977"
                     /product="DUF1430 domain-containing protein"
                     /protein_id="WP_000078816.1"
                     /transl_table=11
                     /translation="MSQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRISSQDE
                     RQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFWIDAVQNY
                     VLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAVLGIATSIL
                     LFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVEIGVA
                     FLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            complement(2383..2655)
                     /locus_tag="A1898_RS11400"
                     /old_locus_tag="SAMEA2521606_01978"
     CDS             complement(2383..2655)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939671.1"
                     /locus_tag="A1898_RS11400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01978"
                     /product="hypothetical protein"
                     /protein_id="WP_000939671.1"
                     /transl_table=11
                     /translation="MLQLEYKNEQSWDSFIDNVNLIELEERIQTTIGIKQINTHNIITI
                     AREGYSQNYLPNTSENTYNSLQVSLVGVLLLFISMVNILWAKKSK"
     gene            complement(2709..4121)
                     /locus_tag="A1898_RS09795"
                     /old_locus_tag="SAMEA2521606_01979"
     CDS             complement(2709..4121)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075: alkaline serine protease, subtilase family"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699968.1"
                     /locus_tag="A1898_RS09795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01979"
                     /product="S8 family serine peptidase"
                     /protein_id="WP_000699968.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 1.2e-38, bitscore:
                     123.5, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIMKKKYWTLAILFFCLFNNSVTAQEIPKNLDGNITHTQTSESF
                     SESDEKQVDYSNKNQEEVDQNKFRIQIDKTELFVTTDKHLEKNCCKLELEPQINNDIVN
                     SESNNLLGEDNLDNKIKENVSHLDNRGGNIEHDKDNLESSIVRKYEWDIDKVTGGGESY
                     KLYSKSNSKVSIAILDSGVDLQNTGLLKNLSNHSKNYVPNKGYLGKEEGEEGIISDIQD
                     RLGHGTAVVAQIVGDDNINGVNPHVNINVYRIFGKSSASPDWIVKAIFDAVDDGNDIIN
                     LSTGQYLMIDGEYEDGTNDFETFLKYKKAIDYANQKGVIIVAALGNDSLNVSNQSDLLK
                     LISSRKKVRKPGLVVDVPSYFSSTISVGGIDRLGNLSDFSNKGDSDAIYAPAGSTLSLS
                     ELGLNNFINAEKYKEDWIFSATLGGYTYLYGNSFAAPKVSGAIAMIIDKYKLKDQPYNY
                     MFVKKFWKKHYQ"
     gene            complement(4145..6253)
                     /locus_tag="A1898_RS09800"
                     /old_locus_tag="SAMEA2521606_01980"
     CDS             complement(4145..6253)
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /locus_tag="A1898_RS09800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01980"
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="WP_000701298.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 4.8e-39, bitscore:
                     123.7, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIPMIYQMENSECGLACCAMILNYFKYEISLNELREIYPSSRSG
                     YSLLSISKVLGDFNISSHAFKASVRDLKPLSFPLICFWESSHFIILEKISKNKFYILDP
                     AKGRQRMSISEFERHYSNIILTFKKLDSFMSRKDNKKSPVLKYFFKYRNKLGILFFVTA
                     LLYVIQSLVPIANRYIIDTNFKDDSYSSRMLFTILFIFTVSFSLMYLLRQIYVASLKYI
                     MDKEISYDFMKHLIYLPYSFYEKRTLGDILFRANSIVYIREILSNNFIAAILDLLMIVV
                     YAVVLFSFSKYMVIFLISLSLALSIVMYPIIKISKNLIDKNIKEKVNVQNITSEVISKN
                     SDIKLTGEEEFWINKWDNFNTKQLIIGRKLDIHLSIVSSITNVLQIILPVLTLIVGVNI
                     KTFEQLTLGQIVAISTVSPYFISPIISLSDNYIQLMLLKGYFLRIEDVFNTKSELIPER
                     VSQDIKFDKKIELKDIWYKYGLFDDYVLKGINVTIKKGETVAIVGESGSGKSTLAKILL
                     GLLEPNIGSIEVDGVEKEEIGQTLYRKIFGAVLQNSTLSYGTLRENLTFGHFVSDEELM
                     TNLNSIGLSNVVKSLPLGLETIIAEEGNNFSGGQQQMILLARCLLSKPSVVVLDEATSS
                     LDNLSQQITTSYLSEIGTTKILIAHRLDTIKSADKILVMHNGEIVEIGTHRELLELGGI
                     YKQLYSNN"
     gene            complement(6250..7179)
                     /locus_tag="A1898_RS09805"
                     /old_locus_tag="SAMEA2521606_01981"
     CDS             complement(6250..7179)
                     /EC_number="3.4.24.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912951.1"
                     /locus_tag="A1898_RS09805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01981"
                     /product="M50 family metallopeptidase"
                     /protein_id="WP_000912954.1"
                     /transl_table=11
                     /translation="MLKILNNSVIYLCLIPLLFLLLIIFPNDSLIYYFRLILISLISIT
                     LHELGHFLVGRCLSYKLEMLATPFFFYFRKKIYFKFPVLLAFGYCQMSNRNITNEKNSD
                     RNLVFYFFGGGGANLIVAILALLGFVPYASEFFILNIILFLVTVCLPIDGTDGNAIREI
                     VLYSKDSKTYQRFFANSLYNNPYITIDDFAKLTSKEKSFFSKFEKCLINLYFEVKGEGS
                     AFTIANHEVFDSNIQENIIHEYYKLLHKSSDWIIPQNLSLEEVVFTLSNYIYSKNNKYL
                     EKIKYLKKLVDFRQEEIIDFILNKEEVL"
     gene            complement(7160..7621)
                     /locus_tag="A1898_RS11405"
                     /old_locus_tag="SAMEA2521606_01982"
     CDS             complement(7160..7621)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015325.5"
                     /locus_tag="A1898_RS11405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01982"
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="WP_079117385.1"
                     /transl_table=11
                     /translation="MIIISSPVYLQNVSVDTKNFIERIGGWSHLFRLAGKFVVTLDVAE
                     SNGSDNVSEYLRDIFSYMGGQILHQVSITNSLKDIAEAQLMEATYKIEDVLEGKIKYKT
                     TDYQERAYQTLKLILENYDSEHFEKMYWEKKRLFEANSLEEWYYVENIK"
     gene            complement(7630..7863)
                     /locus_tag="A1898_RS11410"
                     /old_locus_tag="SAMEA2521606_01983"
     CDS             complement(7630..7863)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A1898_RS11410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMEA2521606_01983"
                     /product="hypothetical protein"
                     /protein_id="WP_001033171.1"
                     /transl_table=11
                     /translation="MNKIFIYAGVRNHNSKTLEYTKRLSSIISSRNNVDISFRTPFNSE
                     LEISNSDSEELFKKGIDRQSNADDGGVIKKSY"
     gene            complement(8213..11203)
                     /locus_tag="A1898_RS09815"
                     /old_locus_tag="SAMEA2521606_01984"
     CDS             complement(8213..11203)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070: lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875571.1"
                     /locus_tag="A1898_RS09815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01984"
                     /product="type 2 lanthipeptide synthetase LanM family
                     protein"
                     /protein_id="WP_000875573.1"
                     /sec_met_domain="DUF4135 (E-value: 5e-92, bitscore: 299.3,
                     seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 4.5e-53, bitscore:
                     170.6, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYKIKNVHKTLFIKERYQILSERNDNSLNNFSLNNWRSTMSIIS
                     EKTFEEMLKLNNYNKKIFSNTVNYDYTEEQIRLYSEYAKNSEWYKFAEGSFEIFRKHPQ
                     RINFKKDFSRILEPFIINLLKLINNYLENKEYEWIDKNGNIFSSLVFYLEDLIYPWIVK
                     PLVLEINSLREKGLLEGESEQQRYKYFITLFDKEENILNFYNKYPVLLRQISESCLRFY
                     TYFIEILSNLENDFSVLEEELGLRGKLNDIKFGKGDTHSQGKTVLILFFDDAKIVYKPK
                     NLIINNSLNTIAEYIRKVDEKIRIRIPRTIAYSDHSYEEFIDYLPLEQKKNLPEYYYNF
                     GVLLAFIYLFNGSDIHFENLISYGDMPVIIDFETMLQQPLFDDKTGQSLLDTLFHRVTR
                     TLLLPTEGVKREDGLDVEMSALTGNFKKDAFNGQVLINLNTDKVKFDIGKIDFEGGKNL
                     PVRDGDIEFDKYIKDFKKGFRDFYLIFEELNKTEEFKMLLKANLYGLKTRVLFRDTNSY
                     ASVLSFLYHPDFYEEMLDREKALENLWSNKFSNQGIVASECEQMRLLDIPIFYTDTNIN
                     EIYDDFGNHFISLHQFSGYDYFMKYLPNNNLENLEKQLDIIEIYFPSFDQRKKGYKLNL
                     SNRKKLPNESLVTILHNEVENIADSLVINNIYDEYPIWSIPVNFKGSKWCLEQTGNDMY
                     RGRAGILLFMYYVKLLKDKSSYTQLYNKMLSAIPPSVSLSKTTFGLTGDIGYFIVLSII
                     EDYDTNTLACLNYIKSVLTELEKTDFASVSNKSDYINGLLPLINTLVRYYRRGIEKERV
                     LRLVLSLGDTLYNDVSERDNSNFGYSFGHGLSGVIFTLKNIYKVTRLKKYKELILQLVP
                     REKIKIHSLKWCSGEFGTLVNHPQSVPEGIVASLDNDTFCHGSMAIINFYIDYMKEMDT
                     SMELESLLCSVVMNKSKEGEYRIVQTSNFPDFSLYTGVTGIAYTFLRYIALNQNDKKIV
                     IPSLLEI"
     gene            complement(11319..11507)
                     /gene="pneA2"
                     /locus_tag="A1898_RS09820"
                     /old_locus_tag="SAMEA2521606_01985"
     CDS             complement(11319..11507)
                     /codon_start=1
                     /gene="pneA2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786759.1"
                     /locus_tag="A1898_RS09820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01985"
                     /product="two-peptide bacteriocin subunit PneA2"
                     /protein_id="WP_000786759.1"
                     /transl_table=11
                     /translation="MKNDFVIGKSLKELSLEEMQLVYGGTDGADPRSTIICSATLSFIA
                     SYLGSAQTRCGKDNKKK"
     CDS_motif       complement(11433..11507)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MKNDFVIGKSLKELSLEEMQLVYGG"
                     /locus_tag="A1898_RS09820_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(11319..11432)
                     /prepeptide="core"
                     /locus_tag="A1898_RS09820_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-1.30"
                     /molecular_weight="3743.2"
                     /monoisotopic_mass="3741.0"
                     /core_sequence="TDGADPRSTIICSATLSFIASYLGSAQTRCGKDNKKK"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MKNDFVIGKSLKELSLEEMQLVYGG"
                     /leader_location="[11432:11507](-)"
                     /alternative_weights="3761.2"
                     /alternative_weights="3779.2"
                     /alternative_weights="3797.3"
                     /alternative_weights="3815.3"
                     /alternative_weights="3833.3"
                     /alternative_weights="3851.3"
                     /alternative_weights="3869.3"
     gene            complement(11514..11738)
                     /gene="pneA1"
                     /locus_tag="A1898_RS09825"
                     /old_locus_tag="SAMEA2521606_01986"
     CDS             complement(11514..11738)
                     /codon_start=1
                     /gene="pneA1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181087.1"
                     /locus_tag="A1898_RS09825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01986"
                     /product="two-peptide bacteriocin subunit PneA1"
                     /protein_id="WP_000181087.1"
                     /transl_table=11
                     /translation="MTNFNSNEKFCGKSLKSLSADEMSLIYGASDGAEPRWTPTPIILK
                     SAAASSKVCISAAVSGIGGLVSYNNDCLG"
     CDS_motif       complement(11652..11738)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MTNFNSNEKFCGKSLKSLSADEMSLIYGA"
                     /locus_tag="A1898_RS09825_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(11514..11651)
                     /prepeptide="core"
                     /locus_tag="A1898_RS09825_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-0.40"
                     /molecular_weight="4315.8"
                     /monoisotopic_mass="4313.2"
                     /core_sequence="SDGAEPRWTPTPIILKSAAASSKVCISAAVSGIGGLVSYNNDC
                     LG"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MTNFNSNEKFCGKSLKSLSADEMSLIYGA"
                     /leader_location="[11651:11738](-)"
                     /alternative_weights="4333.9"
                     /alternative_weights="4351.9"
                     /alternative_weights="4369.9"
                     /alternative_weights="4387.9"
                     /alternative_weights="4405.9"
                     /alternative_weights="4424.0"
                     /alternative_weights="4442.0"
     gene            complement(11841..12011)
                     /locus_tag="A1898_RS11505"
                     /old_locus_tag="SAMEA2521606_01987"
     CDS             complement(11841..12011)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227862.1"
                     /locus_tag="A1898_RS11505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01987"
                     /product="PhrA family quorum-sensing system peptide"
                     /protein_id="WP_001227862.1"
                     /transl_table=11
                     /translation="MRKKRGIKKLVTFALLGVFMFSNTIPYQQFIQKNKQLEIRVQSQK
                     KSNGLDVGKAD"
     misc_feature    11994..12009
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 24.81"
                     /tool="antismash"
     gene            12203..13066
                     /locus_tag="A1898_RS09830"
                     /old_locus_tag="SAMEA2521606_01988"
     CDS             12203..13066
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /locus_tag="A1898_RS09830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01988"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001093641.1"
                     /transl_table=11
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSAD
                     LLFKLSQRLEVPLDYFFNEQIEVKSNLSNFKHLSARLLDDRNYDDLEYLYKIEVERSTF
                     LTLEDRTYLEWIKSIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFYSLV
                     GREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEALETI
                     ELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMKIENYL
                     KELDTV"
     misc_feature    13103..13120
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 22.23"
                     /tool="antismash"
     misc_feature    13166..13179
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.16"
                     /tool="antismash"
     gene            13449..14069
                     /locus_tag="A1898_RS09835"
                     /old_locus_tag="SAMEA2521606_01989"
     CDS             13449..14069
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /locus_tag="A1898_RS09835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01989"
                     /product="hypothetical protein"
                     /protein_id="WP_000839908.1"
                     /transl_table=11
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFVS
                     GICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQEL
                     VDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTIVSL
                     LIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            14155..14598
                     /gene="tsaE"
                     /locus_tag="A1898_RS09840"
                     /old_locus_tag="SAMEA2521606_01990"
     CDS             14155..14598
                     /GO_process="GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /gene="tsaE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /locus_tag="A1898_RS09840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01990"
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="WP_000288232.1"
                     /transl_table=11
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLAK
                     GLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWGNL
                     LGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     misc_feature    14193..14207
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: weak, score: 16.07"
                     /tool="antismash"
     gene            14588..15106
                     /locus_tag="A1898_RS09845"
                     /old_locus_tag="SAMEA2521606_01991"
     CDS             14588..15106
                     /EC_number="2.3.-.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /locus_tag="A1898_RS09845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01991"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_000455537.1"
                     /transl_table=11
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSEE
                     MEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNGLG
                     SLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGKFID
                     VYLMGKLIG"
     gene            15114..16130
                     /gene="brpA"
                     /locus_tag="A1898_RS09850"
                     /old_locus_tag="SAMEA2521606_01992"
     CDS             15114..16130
                     /codon_start=1
                     /gene="brpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /locus_tag="A1898_RS09850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01992"
                     /product="biofilm formation/cell division transcriptional
                     regulator BrpA"
                     /protein_id="WP_000239282.1"
                     /transl_table=11
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGEE
                     TKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDILTR
                     IESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVGGIT
                     VNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQREVI
                     QKVMEKALSLNSIGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQQLQGE
                     GEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVGDEDN"
     misc_feature    16175..16188
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.52"
                     /tool="antismash"
     misc_feature    16176..16189
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.7"
                     /tool="antismash"
     gene            16215..17471
                     /locus_tag="A1898_RS09855"
                     /old_locus_tag="SAMEA2521606_01993"
     CDS             16215..17471
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /locus_tag="A1898_RS09855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01993"
                     /product="competence/damage-inducible protein A"
                     /protein_id="WP_000642706.1"
                     /transl_table=11
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGDN
                     EVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIFFA
                     LRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPMVLN
                     QLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLRLSTK
                     ASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQGKTIAA
                     AESLTAGLFQATVANFSGVSSIFEGGFVTYSLEEKSRMLDIPAKNLEEHGVVSEFTAQK
                     MAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIGGRSRADV
                     RHIAVMHAFNLVRKALLSD"
     gene            17526..18692
                     /gene="recA"
                     /locus_tag="A1898_RS09860"
                     /old_locus_tag="SAMEA2521606_01994"
     CDS             17526..18692
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /gene="recA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /locus_tag="A1898_RS09860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01994"
                     /product="recombinase RecA"
                     /protein_id="WP_001085462.1"
                     /transl_table=11
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGER
                     AEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGIAA
                     FIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVAALV
                     PRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGNPETT
                     PGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAVVEIMY
                     GEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFDEIDKQV
                     RSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            18999..20369
                     /locus_tag="A1898_RS09865"
                     /old_locus_tag="SAMEA2521606_01995"
     CDS             18999..20369
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1086: MATE efflux
                     family protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /locus_tag="A1898_RS09865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01995"
                     /product="MATE family efflux transporter"
                     /protein_id="WP_001036271.1"
                     /transl_table=11
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVGR
                     FLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWILGA
                     LLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLRSIG
                     DSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRKSVPE
                     LLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQTAARR
                     IMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIFLFFASP
                     ALVSFLASSTDGYLIENGSLYLQISSTFYPILSLLLIYRNCLQGLGQKILPLVSSFIEL
                     IGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAILATKVQS"
     gene            20745..>21187
                     /gene="lytA"
                     /locus_tag="A1898_RS09870"
                     /pseudo=""
     CDS             20745..>21187
                     /codon_start=1
                     /gene="lytA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /locus_tag="A1898_RS09870"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /pseudo=""
                     /transl_table=11
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWRK
                     DPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDYRL
                     YIELLRNLADEAGLPKTLDTDDLAGIKTHEYCTNNQPNNHSDH"
ORIGIN
        1 gttttgtcgc ttttcattat aggtcatatg ggactttttt tctacaataa aataggctcc
       61 ataatatcta tagtggattt acccactaca aatattatag agccttaagt gactattagt
      121 ttgtctcgct ccgttaggtg cgagacaaaa aaaacacccg ctatgcgggt gtgcgtcgaa
      181 ggttatacca aaaaaactcc aaacgcgata caataaaggt gttcaatcca attgtaaagc
      241 gaaaggagaa aaatatggca caaaaggttc ttttatcctg ttcttgtttc aattgactat
      301 atttctatac tttttcttcc tttctctgtg aaaatcgcaa gtttttccca ccttttctca
      361 atactgaaac aacatctttc cagaacttca ataatttctt tttgtgttaa atcattattt
      421 aactaaatct ttggaggcaa tacgttttca aagctacttt tttaccatag gttttgagcc
      481 ttcaacacag attactaagc caaaaatgct ctaaattttt gaaattctaa caaattccac
      541 tcaactttat catcatcttt gactttcaca atatcttcct caaaataaca tggaataaag
      601 atagttttct tttaaaacgg ttgtaggagg aaaatctctc ctacactatt attgattctg
      661 agagaaaatt gcaaaatatc atctaccaaa ctttcaatta ttaagatttt ttgcagttta
      721 tctgaattag aatcgcaagg cttctcaata atataattgt cgaatttttt cattctaact
      781 tcctctccat tatttcttcc tttatcaaga aaaagttacc tcaatattaa taaagtacaa
      841 ttctaaatta agtacaattc taaattaact ccaattcttg attcatgata atatttcagt
      901 aactcatcgt gcaattaaaa acaaggattt ttatttcaga tgatccatcg tgaacacttc
      961 atcagccatc tcccaaattg ccggattatg tgttgcgata atgattagcc tattatcatc
     1021 tcgaagagat agcaaaatct ccataatcaa ctgagaggtt gctgggtcta ttgaagctgt
     1081 tggctcatct gccagaataa agggtggatt ctttaagata atttttgcca aggcaacccg
     1141 ttgcgattct ccgcccgata actcaaagat gcgcttatct aggtcaagat aaaccaggcc
     1201 gacctgttct aaagcctgct tctgcctcaa ccgctgttcc gaccgactca acttttgacc
     1261 aatgagacct agcttaaggt tttcttcaat actttggttt tcaattaagc caaagttctg
     1321 gaagaggtag cccaattcgt gacggaaaaa atcacttgat ttataattgg ccaagtcttt
     1381 acctcggtaa aaaatcgtcc catcataagg ttctaatttc ccaatcatgt tcatcaaggt
     1441 tgtttttccg ctaccacttg aaccaattaa ggcatagact tttccagcct caaatgtcat
     1501 tgaaagattc gaaaataact ctcgttctcc aaaagattta ctcacctgtt taagttcaat
     1561 catattaacc tcccttcaaa acaagcatgg acatcttgtt ttctttctgc atttggacat
     1621 gtaactgtaa aagagataga ccagtaaaga gtaacaagac taagaaagca actcctatct
     1681 ctacctgaag aaatacactc gcaacaaatc ccagtaaaaa cacacccagt tgagcaaaga
     1741 gataagtgcg atggatttct aagaacctga gacctgcaat gcgtttgata aaaatggcac
     1801 gtctaaattc ttcaaagtag agcctattca tagtgttaaa caacaagatt gaagttgcaa
     1861 tcccaagcac agctcctgct agcattaccc aacgttccct ctggatatta tccaataatg
     1921 tgatgtagtt gtggtaacct gtttgcattt ctgagaccca attttcaatg ccttgtctct
     1981 ggataagctc ctgggcatca gacaattgat taaagagaac gtagttctgt actgcgtcta
     2041 tccaaaacaa aatggactgt ggaccagttg attggggtgt tataacaatg atgattggat
     2101 ctttcaaaaa ctgctggtaa gaaatagggg tcgtattata cacaaaacga tcctgacctg
     2161 attctaaata acctaccgta gcagtcattt gctgtcgttc atcttgacta gatatgcgac
     2221 tggttaagtc gtcttcaaaa acagatttat aatgttcttc ctctgaacgg aggtgttcag
     2281 gcagcaataa gacaaactcc cctacatcaa ggtgattcat cttttgactc attttagttg
     2341 aactgtagga cttacttttt cagagggctc caaattttat tttcatttac tttttttagc
     2401 ccataaaata tttaccatac ttataaaaag tagtaatact ccaactaaac tgacttgtaa
     2461 tgaattatat gtattttctg aagtgttagg taaataattt tgagagtacc cttctcgggc
     2521 aatagtaata atattgtgtg tgtttatttg tttaattcca atagtagttt gaattctctc
     2581 ttccaactca attaaattaa cattatctat gaaactatcc caactttgtt catttttata
     2641 ttctaattgt aacatattca aatcatatct caatacgttt ggtatattta acacttttat
     2701 accatttttt actggtaatg tttcttccag aattttttta caaacatata attatagggc
     2761 tgatctttta atttgtattt atcaataatc attgcaatcg caccagaaac tttaggagca
     2821 gcaaatgagt ttccataaag atacgtatat cctcctagtg ttgccgaaaa aatccaatct
     2881 tctttatatt tttctgcatt aataaagtta ttaagtccta attctgaaag agataatgtt
     2941 gagcctgcag gcgcatatat tgcatcagaa tcccctttat tgctaaaatc tgataaatta
     3001 cctaagcgat ctatgcctcc gaccgaaatt gtagatgaga aataacttgg aacatcaact
     3061 actaatcctg gttttcttac ttttttgcgt gaactaataa gtttcaataa atctgactga
     3121 tttgatacat ttagggagtc attccctaat gcagctacta taattactcc tttttgattc
     3181 gcgtaatcaa tagccttttt atacttcaaa aatgtttcaa aatcatttgt tccgtcctca
     3241 tattctccat caatcattaa atattgtcca gtactaagat tgataatatc attgccatca
     3301 tctacagcat caaaaattgc ttttacaatc caatctggac tagctgacga cttaccaaat
     3361 attctataga cgttaatatt aacgtgagga tttactccat taatattgtc atcccctaca
     3421 atttgagcta caacagccgt accatgacct aatctatctt gaatatctga tattattcct
     3481 tcctctccct cctcttttcc taaatatcct ttattgggga catagttttt tgagtgattt
     3541 gaaagatttt tcagtaatcc agtattttgt aaatcgactc ctgaatctaa aatagcaatt
     3601 gaaactttag aattactttt agaatataat ttataacttt cgcctccacc agtaacttta
     3661 tctatatccc attcatattt tcttacaatc gacgattcta agttatcttt gtcatgctct
     3721 atatttcctc ctctattatc tagatgagaa acattttcct taattttatt atctaaatta
     3781 tcttcgccta gtaaattatt actttcagag ttaacaatat cgttatttat ttgtggttca
     3841 agttccaatt tacaacagtt tttttctaaa tgtttatctg ttgttacaaa taattctgtc
     3901 ttatcgattt gaatacgaaa tttattttgg tctacttctt cttgattttt attagaatag
     3961 tcaacctgtt tttcatcaga ttcagaaaaa ctttcgctag tctgagtgtg agttatattg
     4021 ccatcaagat ttttaggtat ttcttgagca gtaacagaat tattgaacaa acaaaagaat
     4081 aatatcgcta aagtccaata tttttttttc ataatcttca taaatttacc cttttaatca
     4141 aaaactaatt atttgaatac aattgcttat aaatgcctcc tagttcaaga agttctctat
     4201 gggtcccaat ctctacaatt tcaccattat gcattactaa gatcttatct gcagacttga
     4261 tagtatctag tcgatgggca attaaaatct tagtggtacc gatttcactt aagtaagaag
     4321 ttgtaatttg ttgagataaa ttatctaaac tacttgttgc ttcgtccaaa acaactaccg
     4381 aaggtttcga caaaagacaa cgagctaaaa gtatcatttg ctgctgccct ccagaaaagt
     4441 tattaccttc ttcagcgatg attgtctcta atccaagagg taaagattta actacattgc
     4501 taagaccaat tgaatttaga tttgtcatta attcttcatc tgaaacaaag tgtccaaatg
     4561 tcaaattctc tcttaaggta ccataactta gggttgaatt ttgtaacact gctccaaaaa
     4621 tctttctata caatgtttga ccaatttctt ctttttctac tccatcaact tctattgaac
     4681 caatattagg ttctaataaa cctaataaaa ttttagctaa tgtactctta cctgaacctg
     4741 attctccaac aatagcaaca gtttctcctt ttttaatagt aacatttatt cctttcaaaa
     4801 cataatcatc aaataatcca tatttatacc aaatatcttt taattctatt tttttatcaa
     4861 attttatatc ttgactgact ctttctggaa ttaattcgga tttagtatta aacacatcct
     4921 ctattcttaa aaaatatccc tttaataaca ttaattgtat atagttatca cttaaagaaa
     4981 ttataggaga aataaagtat ggtgagactg tacttattgc tacaatttgt cctaacgtca
     5041 attgttcgaa tgtttttata tttacaccta caataagggt caaaacaggg agaataattt
     5101 gtaaaacatt cgttatacta ctaacaattg ataaatgtat atcaagtttt cgacctatga
     5161 tgagctgttt tgtattaaaa ttatcccatt tgttaatcca aaattcctct tctccagtta
     5221 gcttaatatc actattttta gaaattactt cggaagtaat attttgaaca ttaacctttt
     5281 cttttatatt tttatcaatt aaattttttg agatttttat gattggatac attacaatag
     5341 atagagctag acttagtgat attaaaaaga ttaccatgta cttagaaaag ctaaataaaa
     5401 ccacagcata aaccacaatc attaacaaat caagtatagc tgctataaaa ttatttgata
     5461 gtatttctct tatataaaca atagagttag ctctaaaaag tatatcccct aaagtacgtt
     5521 tttcataaaa actgtaaggt aaatatatca aatgtttcat aaaatcatag ctaatctctt
     5581 tatccattat atattttaag gatgcaacat atatctgtct taataaatac attagtgaga
     5641 atgaaacagt aaatataaat aatatagtaa ataacattct agacgaatac gaatcgtcct
     5701 tgaaattcgt gtcaattatg tatctattag ctataggtac taatgattgt attacataca
     5761 ataatgctgt tacaaaaaat aaaatcccta gcttattcct atacttaaaa aaatacttta
     5821 aaacaggcga cttcttatta tctttacgag acataaagct atctaacttt ttaaatgtta
     5881 aaatgatatt tgaataatgc ctttcaaatt cacttattga cattctctgc ctgccttttg
     5941 caggatctaa aatataaaac ttgtttttac taattttttc aagaataata aaatgagaac
     6001 tctcccagaa gcaaatgagt gggaaactga gcggttttaa atctcttacc gaagctttaa
     6061 aagcatgaga acttatatta aaatctccta aaactttact tatagatagg agagaatatc
     6121 cagatctgga tgatgggtag atttcacgta gttcatttaa agaaatctca tatttaaaat
     6181 agttcaatat catggcacag catgctaatc cacattctga attttccatt tgatatatca
     6241 ttggaatttt cacaatactt cctccttgtt caaaataaag tcgataattt cttcttgtct
     6301 aaaatctact aatttcttta gatacttaat cttttctaaa tatttattat ttttcgagta
     6361 aatatagttt gacaaggtaa aaacaacttc ttctaaagat aagttctgtg gaataatcca
     6421 atcagaagat ttatgtagta atttatagta ctcatggata atattttcct gaatatttga
     6481 atcaaaaact tcatgattag caattgtaaa tgctgaccct tcacctttca cttcaaagta
     6541 aaggtttatc aaacattttt caaattttga aaaaaaagat ttttctttag aggttaattt
     6601 tgcaaaatcg tcgattgtta tataaggatt attatagaga ctgttcgcaa agaatctctg
     6661 atatgttttt gaatcttttg aatacaaaac tatctctcta atagcattgc catctgttcc
     6721 atcaattggt aaacataccg taactagaaa aagaattata ttcaaaataa aaaattctga
     6781 tgcgtacggc acaaatccca aaagtgctaa tattgcgaca attaggttag ctccacctcc
     6841 cccaaagaag tagaacacca aattcctatc actatttttt tcattagtaa tgtttctatt
     6901 actcatttga caataaccga atgctaataa cactggaaat ttgaaatata ttttttttct
     6961 gaaatagaag aaaaagggag tagcaagcat ctctagttta taagataaac atcttcccac
     7021 taaaaaatga cctagttcat gtaatgtaat tgatattaac gaaattaaaa tcaatcgaaa
     7081 ataatagatt aatgaatcat ttggaaaaat tatcaataat aggaacaata acggaatcaa
     7141 acataaatat atgacagagt tatttaatat tttcaacata ataccattcc tctaaactat
     7201 tagcttcaaa aaggcgtttt ttctcccaat acatcttctc aaaatgttcg gaatcataat
     7261 tttctaaaat taattttaat gtctggtaag ctctttcttg ataatccgtt gttttgtact
     7321 taattttccc ttcaagtaca tcttcaattt tataagttgc ctccatcaac tgagcctctg
     7381 caatatcttt gagtgaattg gtaattgaaa cttggtgtaa tatctgtcct cccatatatg
     7441 aaaatatatc tctaagatat tctgacacat tatcagagcc gttactctca gcaacatcta
     7501 atgttacaac aaactttcca gctaatcgaa aaagatggct ccacccccca atcctttcaa
     7561 taaagttttt tgtgtccaca gatacgtttt gtaaatatac aggagaagag ataattataa
     7621 tatcagactc taataactct tttttataac acctccatca tcagcattac tttgcctatc
     7681 aattcctttc ttaaacaact cttctgaatc agaattagat atttctagct ctgaattgaa
     7741 aggtgtcctg aaagatatat caacattatt tctactagaa atgatacttg aaagtctctt
     7801 agtatactct aaagtcttag agttatgatt tcgcactcct gcatatataa atattttatt
     7861 cattttaatt catcctctca atttgaattt agtagatttt tcaagatagt atggtacaaa
     7921 aacagacttt tgttgactca cattattaca tatgttttgt attaaaccaa aatcaatact
     7981 atttttggag taattttgat tttagtttaa aatcatttct ataacagtag catatacctc
     8041 aagccgttta gcaattagaa tagaactttt ctttattata ttattatctc aacgaaaagc
     8101 tacactatta aaaatatttt atagaattac atattaaact agtcaatctt ggtattttta
     8161 tattgcttaa tgagtggaca cctctatttt agaaacaaaa ctataaatta agctagattt
     8221 caagtaatga ggggataact atctttttgt cattctgatt cagtgcgata taccttaaaa
     8281 aagtataagc aataccagtc acacctgtat acaaagaaaa atctgggaaa ttgcttgttt
     8341 ggacgatacg atactctcct tcttttgatt tattcattac aacactacac aataaagact
     8401 ccaattccat actagtatcc atttctttca tgtagtcgat gtaaaaattt attatggcca
     8461 tacttccatg gcaaaatgta tcattatcta aactagctac aattccctct ggaacacttt
     8521 ggggatgatt aactaatgtc ccaaattctc cactacacca cttcaaagaa tgaattttga
     8581 ttttctccct aggaactagt tgtaaaatta attctttata ttttttaagt cttgtcactt
     8641 tataaatatt ttttaatgta aaaattacac ctgatagtcc atggccaaaa ctatatccaa
     8701 aattactatt atctctctcg cttacatcat tatatagcgt atcacctaaa cttaatacta
     8761 gccttagaac acgttccttc tctattcctc tcctataata tcttaccagt gtattaatta
     8821 aaggtagaag accattaata tagtcagact tgtttgaaac acttgcaaaa tcagtctttt
     8881 caagctcagt taaaacactc tttatataat ttaagcatgc gagagtattt gtatcgtaat
     8941 cctctataat ggatagaaca atgaaatatc ctatatcccc agttaaacca aatgtggtct
     9001 tagataaaga aacagatggc ggaattgcag ataacatttt attgtacagt tgagtatatg
     9061 atgatttatc tttcaataat tttacatagt acataaacag taatattcca gctctacccc
     9121 tatacatatc attacccgtt tgttcaagac accatttaga acctttaaaa ttaacaggta
     9181 tactccaaat tggatattcg tcataaatat tattaataac caaagagtct gcaatatttt
     9241 ctacttcatt atgcagaata gtaactaaac tttcatttgg gagttttttt ctattagata
     9301 agtttaattt atatcctttt tttcgctgat caaagcttgg aaaataaatt tcaatgatat
     9361 caagttgctt ttctaaattt tccaaattat tattaggtaa atatttcata aaatagtcat
     9421 atccagaaaa ttgatgtagg gaaataaaat gatttccaaa atcatcgtag atttcattga
     9481 tatttgtatc tgtataaaaa atcggaatat ctaataacct catttgttca cattcgcttg
     9541 ctacaatacc ttgattagaa aacttattgc tccagagatt ttccaatgct ttttctctat
     9601 ctaacatttc ttcataaaaa tcaggatgat ataaaaaaga tagtactgaa gcatagctat
     9661 ttgtgtctct aaaaagtacc cttgtcttta aaccatacaa gtttgctttt aatagcattt
     9721 taaattcttc tgttttattt aactcttcaa atatcagata aaaatcccta aaaccttttt
     9781 tgaaatcttt tatatactta tcaaattcta tatcaccatc ccgaacaggc aggtttttcc
     9841 caccttcaaa atcaattttc ccaatatcaa actttacctt atcagtattt aaattaatta
     9901 aaacttgacc attaaaagca tccttcttaa aatttcctgt aagagcactc atctcaacat
     9961 caagaccatc ctcccttttt actccctctg ttggaagaag caaagttcga gtcaccctgt
    10021 gaaacagggt atccagcaaa ctctgaccag ttttatcatc aaatagaggt tgctgtaaca
    10081 ttgtttcaaa gtctattatt acaggcatat ctccatagga aattaaattt tcaaaatgta
    10141 tatcactccc attaaataaa tatataaatg ctaaaagcac accaaaatta taataatatt
    10201 caggtaaatt tttcttttgc tctagaggta gataatcaat aaattcttca tagctgtgat
    10261 ccgaataagc aatagttcga ggtattctta tcctaatttt ttcatcaacc tttcggatat
    10321 actcagcaat agtatttagt gagttattga ttattaaatt tttaggcttg taaacaattt
    10381 tcgcgtcatc aaagaagagt atcaaaacag tttttccttg gctgtgtgta tcaccctttc
    10441 caaattttat atcatttaat ttccccctta gccctaattc ttcttctagc acactaaaat
    10501 cattttctaa atttgataaa atttctataa aataagtata gaaccgaaga caagactccg
    10561 atatttgcct cagtaaaacg ggatatttgt tataaaaatt taatatattc tcttccttgt
    10621 caaacaatgt tataaaatat ttgtaccgtt gctgctccga ttccccttca agtaaacctt
    10681 tttcacgcaa tgaatttatc tctaaaacca aaggtttaac aatccaagga tagattaaat
    10741 cttctaaata aaatactaga gaggaaaaaa tatttccatt cttgtcaatc cactcgtatt
    10801 ctttattctc tagataatta ttgattaatt taagcaaatt aataataaag ggttccaaaa
    10861 ttctagaaaa atctttctta aaattaattc tttggggatg ttttctaaat atttcaaatg
    10921 aaccctccgc aaatttatac cattccgaat ttttggcata ttctgaatat agtcttatct
    10981 gttcctcggt ataatcatag ttcacagtgt tagaaaatat ttttttattg taattattta
    11041 atttcaacat ttcttcaaaa gttttttcag aaataattga cattgtagaa cgccaattat
    11101 ttaatgaaaa gttatttaag gaattatcat ttcgttcaga taatatttga tatctttcct
    11161 taataaacaa tgttttatgc acatttttaa ttttatattt catttactta cctcaaacct
    11221 atacgactta ctattgtttt aaaaaaaaac ctctctcaaa attattagtt acgagagagg
    11281 aaaataaaga gcaactttta tttttaaaat tgaatatttt attttttctt gttgtctttg
    11341 ccacaacgag tctgagctga ccccaaatat gatgcaataa aacttaatgt agcagaacat
    11401 ataattgttg atctagggtc agcaccatct gtgccaccat aaaccaattg catttcttca
    11461 agagataatt ctttcaaact ctttccgatt acaaaatcat ttttcatttt aatttacccc
    11521 aaacaatcat tattgtagct aactagcccc ccaataccag aaacagctgc actaatgcat
    11581 actttcgagc tagctgcagc tgatttcaaa ataataggag taggcgtcca acgtggctct
    11641 gcaccatcag atgcaccata aattaaactc atttcatcag cagacaatga ttttaaactt
    11701 tttccacaaa atttttcgtt tgaattaaag ttggtcataa gtaataactc ctccttataa
    11761 aaagttaaat aagttgctct atatttttcg ttttttcaga taaattataa ttctctcatt
    11821 ctcgtgcaaa atatcgggtt ttaatccgcc ttcccaacat caagaccatt ggacttcttt
    11881 tgcgattgca ctcgaatctc caattgttta ttcttctgaa taaactgttg gtaaggaatt
    11941 gtattactaa acataaaaac acctagcaat gcaaatgtaa ctaatttttt aattccacgt
    12001 ttttttctca tgcaatgaaa cccctttctt tttccttgtt tttattctaa cagatatatt
    12061 ttatttagtc aagtattttt atgtaatttt taactttttt ttttgtttat taccataaag
    12121 aattatttat tcctttaact tgctattttg aactaaaaac tttataatga aattaagcaa
    12181 ataggaaggt ttattatctt taatgaatac actcgcagag aaattcagat tgaaaagaaa
    12241 agagttgaga ctctcccaac aaactcttgc agaaggaatt tgtgaacaaa gccagattag
    12301 taaaattgaa agaggtcatt ttattccctc cgcagacttg ttattcaaac tctctcaacg
    12361 acttgaagtc cctttggatt actttttcaa tgaacaaatc gaagttaagt ctaacctctc
    12421 taatttcaaa catctatctg ctcgcctatt agatgacaga aattatgacg atttagaata
    12481 tctttataaa atagaggtag aacgaagcac ttttctaaca ttagaagacc gaacttacct
    12541 tgaatggatt aaatctataa ttgacttcta tcaatatgac agtaagtgtg aggcaatttc
    12601 gtcattggaa aatatattat taaaagtctc ttcaaatact ctgatttatt taaaggcgtt
    12661 gaatactcta tctaatttct attccttagt gggtcgtgaa caagaatatg aggcaaacta
    12721 ctctcattta atagagttgt atcagacaaa aaatttggac catcaagagt ttttatttgg
    12781 ctacatcaga gttcgttaca actacgctca ctacctagta tcaaaggaaa aatataatga
    12841 agccatccaa gaagctcttg aaacgattga actctgtaaa caaagacaga caagctacca
    12901 attggctcct ctacttattc ttgtaggaaa tgctggagcc aaatttctag acagagaaca
    12961 agtcaaaaat tattatatag aagcaagaga gttatgtaag atttataaca atcctttaat
    13021 gttgatgaag atagaaaatt atttgaagga actagatact gtttagttaa tcttgacatt
    13081 atagtttttc tgaaacgtta aataacctgt aaggctgata gttagattaa tactatcagc
    13141 cttataatta tgtgactctg agtccgtctc gctccgtgag gtgcgagaca aaacaaccac
    13201 ccgtttgacg gattttcatg atttcaaatt tatattcttc gaaaatctct tcaaatcacg
    13261 tcagctctat ctgcaatctc aaaacactgt tttgagcaac ctgtggctag ctacctagtt
    13321 tgatctttga ttttcattga gtattagtct ctagtgtttg tttagcgtct taaagagaca
    13381 tttgagaaga ttagaaacag ttctctcttt tctcctagta taatgattgg taggaaggga
    13441 gagagaagat gaaatcaatg agaatcttat ttttgttagc tttaattcaa atcagtttga
    13501 gtagctgttt cctatggaag gaatgcatct tgtcctttaa acaaagtaca gcttttttca
    13561 tcggaagcat ggttttcgtt tcaggaatct gtgctggagt aaattatctt tatacccgta
    13621 agcaagaagt ccatagtgtc ctagccagta agaagtcggt gaagcttttt tacagtatgt
    13681 tactcttaat taatttgtta ggagctgttc ttgttttgtc agataacttg ttcatcaaaa
    13741 atacgctgca gcaagaatta gttgactttt tattgccatc cttctttttc ctatttgggc
    13801 tagatttgct gattttttta cccttgaaaa aatacgtgcg cgattttctt gctatgctgg
    13861 acagaaaaaa gacagtgttg gtgactattt tagcaacact tcttttctta agaaatccaa
    13921 tgaccattgt ctcacttctg atttatattg gactgggctt gttttttgca gcctatcttg
    13981 tcccaaattc ggttaagaag gaagtttcct tttatggtca tattttccga gatcttgtat
    14041 tggtcattgt tacgctcatt ttcttttaga gaaagcctgt tttttatgga tgttatgatg
    14101 ataagtttag taggaaaata gacacaaaag aaaagttttt ggtataataa gagtatgtac
    14161 acaaaaaatg aagaagagtt gcaagcctta ggggagcgtt tgggccatct attagcaaag
    14221 aatgatgttt taatcttaac tggagaactg ggtgcaggta aaacgacctt tactaaagga
    14281 cttgcaaaag gattacagat ttctcaaatg attaaaagtc ccacctatac tatcgtgaga
    14341 gagtatgaag gtcgacttcc actttatcac ctagatgttt atcgtattga aggagatgct
    14401 gattctatcg acttggatga gtttatcttt ggtggcggcg tgactgttat tgagtgggga
    14461 aatctcttag gagatgcctt gccagatgct tatttagaat tggaaattct aaaagaagca
    14521 gatggacgcc gtttaaattt tcaggcaaag ggtttgcgtg ctgagaaatt gttagaggag
    14581 cttcaatatg gagtatgaat tgctcattag ggaagcagag cccaaagatg cagctgaatt
    14641 ggtggccttt ttaaatcgtg tgagtttgga gacagacttt accagcctag acggagatgg
    14701 tattctcttg actagtgagg agatggaaat attcctcaac aagcaagcta gttcggacaa
    14761 tcagataacc ttacttgcat tcttaaatgg caaaattgct ggtattgtaa atattacagc
    14821 tgatcagcgt aagagagtcc gtcatattgg agatctcttc attgtgattg gaaaaagata
    14881 ttggaataat ggcttgggaa gtttgttgct agaagaagcg atagagtggg cacaagcaag
    14941 tggcattctg cgtcgtctcc aactgactgt ccaaactcgt aatcaagcag cagtccatct
    15001 ttatcaaaag catggctttg tcattgaagg tagccaagag cgtggggcat atatagaaga
    15061 agggaaattt atcgatgttt acttgatggg taaactgata ggttagtaga aatatggtta
    15121 aaaaaattat tggaatggtg ctagctttac tttctgtaac tgtagtagga gtaggtgttt
    15181 ttgcttatac tatttatcaa caagggacag aaaccttagc taaaacctat aaaaaaatcg
    15241 gtgaagaaac caaggttatt gaagcgactg aacctctaac cattctgtta atgggagtgg
    15301 acaccggaaa tgttgaacga actgaaactt gggtcggtag aagtgatagc atgatcttga
    15361 tgacagtgaa tcctaaaacg aaaaaaacaa caatgatgag tttagagcgg gatattctga
    15421 cgcgcattga atcagggaat ggtcaggctc atgaagcgaa actgaactca gcatatgcag
    15481 atggtggagc agagcttgct atagaaacca ttcaaaaaat gatgaatatc catattgatc
    15541 gctatgtgat ggtcaatatg agagggttgc aaaaattagt ggatgcagta ggaggtatta
    15601 cagtcaataa tatcctaggt ttcccaattt ctatcagtga ccaagaagaa tttaatacca
    15661 tttctatcgg tgttggggag caacatattg ggggagaaga agccctagtc tatgcacgaa
    15721 tgcgttacca agatcctgag ggggattatg gtcgtcaaaa acgtcaacgt gaagttattc
    15781 aaaaagtcat ggaaaaagct ctcagtttaa atagcattgg tcattatcaa gagattctaa
    15841 aagctttgag tgacaatatg cagaccaata ttgatttgtc tgcaaaaagt atccctaact
    15901 tgctaggcta taaagattca tttaaaacca ttgaaactca gcagttgcag ggtgaaggag
    15961 agatacttca aggtgtttct taccagattg tttcgagagc acatatgttg gaaatgcaaa
    16021 atatactccg acgttctttg ggacaagaag aagttactca gcttgaaacc aatgcggttt
    16081 tatttgaaga tttatttggc agagcacctg ttggtgatga agataattaa cttttgatac
    16141 aaataaaaaa atcaatcgta ggaaaattcc tgcgattttt tcaaaaaaat acgaatagat
    16201 aggtaggagg aaacatgaaa gcagaaatca ttgctgttgg aacagagatt ttgacaggac
    16261 agattgtcaa caccaatgcc cagtttttgt cggaaaaact agctgagatt ggggtagatg
    16321 tatattttca gacggctgta ggagacaatg aagttcgtct cttgtctttg cttgagattg
    16381 ccagtcaacg tagcagtctg gtgattttga caggcggttt ggggccaact gaggacgacc
    16441 taaccaaaca aaccctagct aaatttttag ggaaagcatt agtctttgat cctcaggctc
    16501 aggagaagtt ggatatcttt tttgccctgc gaccagacta tgcccgaaca ccgaataacg
    16561 aaagacaagc tcaaattgta gaaggagcga ttccactgcc aaacgaaaca ggactggctg
    16621 ttggaggaaa attagaagta gacggagtga cctatgtcgt ccttccaggt ccgccaagtg
    16681 aattgaaacc catggtctta aaccaacttc tacccaagtt gatgacaggg agcaagctgt
    16741 attcccgagt tcttcgtttc tttgggattg gcgagagcca gttggttacg attttggctg
    16801 atttaattga taatcagatc gatcctacct tggcccctta tgccaagaca ggagaagtca
    16861 ctctacgtct gtcaacaaag gctagcagtc aagaagaggc gaatcaagcg ctggatatct
    16921 tggaaaatca aatcttggac tgccagactt tcgaaggaat ttctttacga gacttttgct
    16981 atggttatgg ggaagaaact agtttagcca gcattgtggt agaagaactg aaaaggcaag
    17041 ggaaaaccat cgcggctgca gagagtttga cggcaggtct tttccaagct accgtggcga
    17101 atttttctgg agtttcaagt atatttgagg gtggttttgt gacctatagc ttggaggaaa
    17161 aatcaaggat gttggatatt cctgccaaga atttggaaga acatggtgtg gtgtctgaat
    17221 ttacagctca gaagatggct gagcaggcac gaagcaagac ccagtctgat tttggaatta
    17281 gtttgactgg agtggcagga ccagatagcc tagaaggaca cccagttggg acagtcttca
    17341 taggcttggc gcaagatcaa ggaactgagg ttatcaaggt taatattgga ggcagaagcc
    17401 gagcagatgt acgtcacatt gcggttatgc atgcctttaa cctagttcgc aaggctttat
    17461 taagtgacta acttttgata taatagtaga taggtctgag gatcattaga atgtaggaga
    17521 atagaatggc gaaaaaacca aaaaaattag aagaaatttc aaaaaaattt ggggcagaac
    17581 gtgaaaaggc cttgaatgac gctcttaaat tgattgagaa agactttggt aaaggatcaa
    17641 tcatgcgttt gggtgaacgt gcggagcaaa aggtgcaagt gatgagctca ggttctttag
    17701 ctcttgacat tgcccttggc tcaggtggtt atcctaaggg acgtatcatc gaaatctatg
    17761 gcccagagtc atctggtaag acaacggttg cccttcatgc agttgcacaa gcgcaaaaag
    17821 aaggtgggat tgctgccttt atcgatgcgg aacatgccct tgatccagct tatgctgcgg
    17881 cccttggtgt caatattgac gaattgctct tgtctcaacc agactcagga gagcaaggtc
    17941 ttgagattgc gggaaaattg attgactcag gtgcagttga tcttgtcgta gtcgactcag
    18001 ttgctgccct tgttcctcgt gcggaaattg atggagatat cggagatagc catgttggtt
    18061 tgcaggctcg tatgatgagc caggccatgc gtaaacttgg cgcctctatc aataaaacca
    18121 aaacaattgc catttttatc aaccaattgc gtgaaaaagt tggagtgatg tttggaaatc
    18181 cagaaacaac accgggcgga cgtgctttga aattctatgc ttcagtccgc ttggatgttc
    18241 gtggtaatac acaaattaag ggaactggtg atcaaaaaga aaccaatgtc ggtaaagaaa
    18301 ctaagattaa ggttgtaaaa aataaggtag ctccaccgtt taaggaagcc gtagttgaaa
    18361 ttatgtacgg agaaggaatt tctaagactg gtgagctttt gaagattgca agcgatttgg
    18421 atattatcaa aaaagcaggg gcttggtatt cttacaaaga tgaaaaaatt gggcaaggtt
    18481 ctgagaatgc taagaaatac ttggcagagc acccagaaat ctttgatgaa attgataagc
    18541 aagtccgttc taaatttggc ttgattgatg gagaagaagt ttcagaacaa gatactgaaa
    18601 acaaaaaaga tgagccaaag aaagaagaag cagtgaatga agaagttccg cttgacttag
    18661 gcgatgaact tgaaatcgaa attgaagaat aagctgttaa agcagtggag aaatccgcta
    18721 ctttttcgat ttttgattca agtttttaga ttatatatag tagcttgaaa taagatatga
    18781 acaactttat taggaaagtc aaattaattt ctagaaatgt tttagcagct acagcgtact
    18841 attccaaact caaccaacta taatagatcg aaactagaat agtacacatc tacttctaaa
    18901 acattgttaa aaatcgattt gactgtcctt atttcattcc gctatatata agtttgctgt
    18961 ttcttgtcgc tcctctggaa agctgatata atagctttat gaataaaaaa cgaacagtgg
    19021 acctgataca tggtccgatt cttccctcgc tcttaagctt cacctttcca atcttgctat
    19081 caaatatttt tcaacagctc tataacactg ctgatgtctt gattgttgga cgatttcttg
    19141 gtcaagaatc cttggctgca gtaggagcga cgacagcgat ttttgacctg attgtaggct
    19201 ttacacttgg tgttggcaat ggcatgggga ttgtcattgc tcgttattat ggggctcgga
    19261 atttcactaa aatcaaggaa gcagtagcag ccacctggat tttaggtgct cttttgagca
    19321 ttctagttat gttgctgggc tttcttggct tgtatcctct cttgcaatac ttagatactc
    19381 ctgcagaaat tcttcctcaa tcttatcaat atatttctat gattgtgacc tgtgtaggtg
    19441 tcagctttgc ttataatctt tttgcaggct tgttgcggtc tattggtgac agtctagcag
    19501 ccctgggatt tctgattttc tctgccttgg ttaatgtggt tctggatctc tattttatta
    19561 cgcaattgca tctgggagtt caatccgcag gacttgctac cattatttcg caaggtttat
    19621 cagcggttct ctgcttttat tatattcgta aaagtgtgcc agaactcttg ccacagttta
    19681 aacatttcaa atgggacaaa agcttgtacg cggatctctt ggagcaaggt ttggctatgg
    19741 gcttgatgag ttcaattgta tctatcggca gtgtgatttt acagttttct gttaatacat
    19801 ttggtgcagt gattattagt gcccagacgg cagctcgacg cattatgacc tttgcccttc
    19861 ttcctatgac cgctatttct gcatcaatga cgacctttgc ttctcagaat ctaggagcta
    19921 agcgacctga ccgtattgtt caaggtcttc gaatcggcag tcgtttaagt atatcctggg
    19981 cagtttttgt ttgtattttc ctcttttttg ccagtccagc tttggtttcc ttcttggcta
    20041 gttcgacaga tggttacttg atagaaaatg gaagtctcta tctgcaaatc agttcaacct
    20101 tttatcccat tttgagcctc ttgttgattt atcgcaattg cttgcagggc ttggggcaaa
    20161 agatccttcc tctagtttct agctttattg aactaatcgg aaaaatcgtt tttgtggttt
    20221 tgattattcc ttgggcagga tataagggtg ttatcctttg tgaacctctt atctgggttg
    20281 ccatgacagt tcaactgtac ttctcactat tccgtcatcc cttgataaaa gaaggcaagg
    20341 caatcttggc aaccaaagtg caatcctagt tggatttact gaataaaatc catttcctct
    20401 agtgaaaatc gaaaaaactt gtgttctcgt ctttagtttg gtgttgaaaa tagtttaaca
    20461 gacttttgac ttcttttata tgatataata aagtatagta tttatgaaaa ggacatatag
    20521 agactgtaaa aatatacttt tgaaaagctt tttagtctgg ggtgttattg tagatagaat
    20581 gcagaccttg tcagtcctat ttacagtgtc aaaatagtgc gttttgaagt tctatctaca
    20641 agcctaatcg tgactaagat tgtcttcttt gtaaggtaga aataaaggag tttctggttc
    20701 tggattgtaa aaaatgagtt gttttaattg ataaggagta gaatatggaa attaatgtga
    20761 gtaaattaag aacagatttg cctcaagtcg gcgtgcaacc atataggcaa gtacacgcac
    20821 actcaactgg gaatccgcat tcaaccgtac agaatgaagc ggattatcac tggcggaaag
    20881 acccagaatt aggttttttc tcgcacattg ttgggaacgg ttgcatcatg caggtaggac
    20941 ctgttgataa tggtgcctgg gacgttgggg gcggttggaa tgctgagacc tatgcagcgg
    21001 ttgaactgat tgaaagccat tcaactaaag aagagttcat gacggactac cgcctttata
    21061 tcgaactctt acgcaatcta gcagatgaag caggtttgcc gaagactctt gatacagacg
    21121 acttggcagg tatcaagacg catgaatact gtaccaataa ccaaccaaac aaccactcag
    21181 accatgt
//
