LOCUS       NZ_FHRS02000020        13217 bp    DNA     linear   CON 12-DEC-2024
DEFINITION  Streptococcus pneumoniae strain GPSC18 substr. ST1797
            ERS455146SCcontig000020, whole genome shotgun sequence.
ACCESSION   NZ_FHRS02000020
VERSION     NZ_FHRS02000020.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2016) WTSI, Wellcome Trust Sanger Institute, CB10
            1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900055005.2-RS_2024_12_12
            Annotation Date                   :: 12/12/2024 02:43:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,241
            CDSs (total)                      :: 2,189
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 73 of 180
            Pseudo Genes (incomplete)         :: 93 of 180
            Pseudo Genes (internal stop)      :: 57 of 180
            Pseudo Genes (multiple problems)  :: 36 of 180
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:31:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 13217
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FHRS02000020.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..13217
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..13217
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13217
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[292:8217](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      293..8217
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            <1..90
                     /locus_tag="A1898_RS08265"
                     /pseudo=""
     CDS             <1..90
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /locus_tag="A1898_RS08265"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="IS5/IS1182 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="FSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            293..523
                     /gene="blpU"
                     /locus_tag="A1898_RS08270"
                     /old_locus_tag="SAMEA2521606_01674"
     CDS             293..523
                     /codon_start=1
                     /gene="blpU"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /locus_tag="A1898_RS08270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01674"
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="WP_001093487.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 1.1e-17,
                     bitscore: 55.9, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTKTMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAARGLQL
                     GIKTRTWQGAATGAVGGAILGGVAYAATCWW"
     gene            526..651
                     /locus_tag="A1898_RS11500"
                     /old_locus_tag="SAMEA2521606_01675"
     CDS             526..651
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842754.1"
                     /locus_tag="A1898_RS11500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01675"
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="WP_000346297.1"
                     /transl_table=11
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKKFLKSSEKKTEKSVKK"
     gene            1062..1256
                     /locus_tag="A1898_RS11355"
     CDS             1062..1256
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757183.1"
                     /locus_tag="A1898_RS11355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_000756776.1"
                     /transl_table=11
                     /translation="MKKVLKYFLVFVFLFLMNIFIFKILATLGFQLIMSEKSYIVLPLF
                     SLIVLYMIDKRIRKKKKSL"
     gene            1277..2170
                     /locus_tag="A1898_RS08275"
                     /old_locus_tag="SAMEA2521606_01676"
     CDS             1277..2170
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /locus_tag="A1898_RS08275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01676"
                     /product="XRE family transcriptional regulator"
                     /protein_id="WP_000343888.1"
                     /transl_table=11
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENGQ
                     ALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKEAI
                     LEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFSVND
                     LLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKASLVQMEKVLLSLFIQIQTEDS
                     LIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNILEAESFYEKAISLAELLDDQV
                     LVQRILAEKQIDFPTT"
     misc_feature    2222..2233
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.13"
                     /tool="antismash"
     gene            2701..2997
                     /locus_tag="A1898_RS08280"
                     /old_locus_tag="SAMEA2521606_01677"
     CDS             2701..2997
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IId"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /locus_tag="A1898_RS08280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01677"
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="WP_000837726.1"
                     /sec_met_domain="Bacteriocin_IId (E-value: 1.5e-22,
                     bitscore: 71.2, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTKA
                     AATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
     gene            3004..4140
                     /locus_tag="A1898_RS08285"
                     /old_locus_tag="SAMEA2521606_01678"
     CDS             3004..4140
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698829.1"
                     /locus_tag="A1898_RS08285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01678"
                     /product="hypothetical protein"
                     /protein_id="WP_000698829.1"
                     /transl_table=11
                     /translation="MKILSKKECESILGYLNFIGITLLFFVIISLSIYFKSYLPFQGKL
                     FLLFLVVDYATLNRYNMKLPIQSKKINTFYIRKNYIFLYLLAIVKKYYSFQILQSFIYL
                     FILYFLDIIGYFYLSITLCLIAIQIISLILPSLFSIISKLFLFLSIGLLSIEQGQVVIY
                     VILLNYFILVMGILDNHASNFYFKSSRMSQKRFRSSLFILLRFCWLNKGLTFSFILISI
                     LLSYLFWYMMTMLSIDDTKIPILFLYTSIYITLMEAIIGNHKSSIGVDLALLQLSALNH
                     SKMNFFFKNEQLMMLTHLISFLQFSLLLCLIPVTSYSNDIKSILLNLLLIPFNYFIAYV
                     YYKKALFLIKGTVSLLRWSFLVLYCFLIIFVILVGGIR"
     gene            4137..4619
                     /locus_tag="A1898_RS08290"
                     /old_locus_tag="SAMEA2521606_01679"
     CDS             4137..4619
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232094.1"
                     /locus_tag="A1898_RS08290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01679"
                     /product="stage II sporulation protein M"
                     /protein_id="WP_001232094.1"
                     /transl_table=11
                     /translation="MRKSGFLIYYVLFCIVCFLLGLLLSFILKNETFATIPPKLELQFT
                     LTVLQANSTNFLLYLFCFPISPLLQLFDLLSLGIQLGISGQMIGIVATIKGILPHALIE
                     IPNFIWYQGMSQYLLYRLLKDRGIKNILIYIKSNLKWYIISYLLLILAALIEGIKL"
     gene            4616..5212
                     /locus_tag="A1898_RS08295"
                     /old_locus_tag="SAMEA2521606_01680"
     CDS             4616..5212
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687786.1"
                     /locus_tag="A1898_RS08295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01680"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_000687787.1"
                     /transl_table=11
                     /translation="MKIENESKSYDKCILKNISLEIDKGIYRVEGENGSGKSTLLQLLA
                     GLETFDSGLKNCVSKDVLYLDTNQLGVVPFTIEENLQLLCDSFQIHLSFEDKEQINTFF
                     DNKIGDNYMTASTGTKFKLGLSLIFAKNWDYIFIDETLSTVDARSIDMIAKRLISMKES
                     STIIYVSHNLLNQELISKSQRILIEEGRVYEISNK"
     gene            5193..5684
                     /locus_tag="A1898_RS08300"
                     /old_locus_tag="SAMEA2521606_01681"
     CDS             5193..5684
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671992.1"
                     /locus_tag="A1898_RS08300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01681"
                     /product="hypothetical protein"
                     /protein_id="WP_000671990.1"
                     /transl_table=11
                     /translation="MKFQTNNSLYKLMNLIIYLFLLYLFIDLLMKSGILDEQIDKLSEL
                     SNLFTIKIETILTVISVVFTFIATLIEYGFSLFLITLFFDKNKRNFGDILLSKNITLSF
                     SILLCYLFNPSDIKFAYFSVLTVLLIGITNYARFKNLRLTVVFSVPFLFNAIMSIINSF
                     "
     misc_feature    complement(5769..5782)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: strong, score: 24.43"
                     /tool="antismash"
     gene            6064..8217
                     /gene="comA"
                     /locus_tag="A1898_RS08305"
                     /old_locus_tag="SAMEA2521606_01682"
     CDS             6064..8217
                     /codon_start=1
                     /gene="comA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /locus_tag="A1898_RS08305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01682"
                     /product="peptide cleavage/export ABC transporter ComA"
                     /protein_id="WP_000668296.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 9.7e-44, bitscore:
                     140.0, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 0.0, bitscore: 1125.0,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKTT
                     MDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTGQD
                     KDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPILVK
                     QRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYILQQIL
                     SYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSIIDALAS
                     TILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNRDTMEAN
                     AVLSSSIIEDINGIETIKSLTSESQRYQKIDKKFVDYLKKSFTYSRAESQQKALKKVAH
                     LLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQTAQVANNRL
                     NEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVPQGSKVAFVG
                     ISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQPYVFNGTILE
                     NLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISGGQRQRIALARA
                     LLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAERTEKVVVLDQGK
                     IVEEGKHADLLAQGGFYAHLVNS"
     gene            8230..9579
                     /gene="comB"
                     /locus_tag="A1898_RS08310"
                     /old_locus_tag="SAMEA2521606_01683"
     CDS             8230..9579
                     /codon_start=1
                     /gene="comB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /locus_tag="A1898_RS08310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01683"
                     /product="competence pheromone export protein ComB"
                     /protein_id="WP_000801616.1"
                     /transl_table=11
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKEM
                     SLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESYAS
                     QLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQNETI
                     ASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGTSLAGQNLAYSLYQSYKSQGE
                     ENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQHLAKV
                     GQELTLLAQKILEAESGKKVQGNLLDKGKVTASEDGVLHLNPETSDSSMVAEGALLAQL
                     YPSLEREGKAKLTAYLSSKYVARIKVGDSVRYTTTHDAGNQLFLDSTITSIDATATKTE
                     KGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            9749..10456
                     /gene="purC"
                     /locus_tag="A1898_RS08315"
                     /old_locus_tag="SAMEA2521606_01684"
     CDS             9749..10456
                     /EC_number="6.3.2.6"
                     /GO_function="GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="purC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312208.1"
                     /locus_tag="A1898_RS08315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01684"
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="WP_000043309.1"
                     /transl_table=11
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAGK
                     GVLNNQISSFIFEKLNVAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSKRF
                     GVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINELLKV
                     WFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLGELTD
                     VYEIVWEKLQELK"
     gene            10458..10601
                     /locus_tag="A1898_RS11645"
     CDS             10458..10601
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605805.1"
                     /locus_tag="A1898_RS11645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_050167432.1"
                     /transl_table=11
                     /translation="MFATENLRLSTKRTQAEKVPQTFSLCRELGELTDVYEIVWEKLQG
                     LK"
     misc_feature    12143..12153
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
ORIGIN
        1 ttttcaacaa cctatcgaaa tcatcgtaaa cgcttcggat tacgaatgaa tttgattgct
       61 ggtattatca atcatgaact aggattctag ttttgcagga agtctaatag taaaaaagtg
      121 attagaaaac atctttttta aaaatagaga tgattttgaa acaaaaaagc taattcaaga
      181 cgtttcgatg ccaattcaag atttggatga aaaaaattaa tagatactgt tatactaaac
      241 ttgtcaagtt tgtaacaaga caaatattaa aaataaaaaa gaggtattcg ttatgaatac
      301 aaaaacgatg tcacaatttg aaattatgga tactgagatg cttgcttgcg ttgaaggtgg
      361 cggatgcaat tggggagatt ttgccaaagc aggtgttgga ggaggagcag cacgaggtct
      421 tcagctagga attaaaacaa gaacatggca aggtgcagca actggtgctg tgggaggagc
      481 tatacttgga ggtgtggcct atgcagcgac atgttggtgg taattatgga ttttaaaagt
      541 tttattattg gtttagtagt tggtatattt ggtccttata tggatgattt aattagaaaa
      601 aaatttttaa agtcttcgga gaagaaaaca gaaaaatctg ttaaaaaata atcaaaacta
      661 taaatgatga atctgaatca aaattatttt gcgcatgtaa ataggagtct tatagtaacg
      721 agtcaaaaaa ggagtaacta tgaatcgtaa tttagaacgg tgttatctat tctgactagg
      781 aatagatcat accagaggtg gcttagaaat agcggggaca ttagaaattg aaggaacttt
      841 aaataggatg tcgtaaatgt tgctatcaat gatttatttg tcccaagctt gcctagggtg
      901 tcagtaaaaa atcattttcc tttcatatca tatttttagt aggtaggatg tttgttctgt
      961 ctattttttg ccaaaaaaag tgcagtcgaa gtgtttattt ggagttttat atatattttc
     1021 gataaaatag gtgtagaaat taggaaagtt gaagaaaaga aatgaagaag gttttaaaat
     1081 attttttagt atttgttttt ctatttttga tgaatatttt catctttaag atactagcaa
     1141 ccctgggatt tcagctgata atgagtgaaa agagttatat tgttctaccg ctgttttcgc
     1201 ttatcgtttt atatatgatt gacaaaagaa ttaggaagaa aaagaaatct ttgtaatcaa
     1261 cttaaggagt attactatgg atgagttgaa acgtgaaatt ggttataaaa tcaaaactat
     1321 taggcgatct tgtggaaaaa ctcagataga tatttgtggt gatgaatcag agttgacgat
     1381 tcgtcagcta gcacgtattg aaaatgggca agctttagca acacttccta aattattatt
     1441 gattgcggat aaacttggag tatcattaca gaatattgtg gatgttaagg ttatagagat
     1501 tccaaaagga ttcctaaaac tcaaagatga gctgattcat tctcaaacct acgctgataa
     1561 gggaagaatc gaacgaaaag aagctatact tgaggaaata tatgaaaact attatgagaa
     1621 tttgcctgag gaagagcagt taattgtgga tgtgacacaa gctcgttttg atatttatgg
     1681 tagttcagat gtgacctacg gtttagggtt agtggaagaa tatttccagc agttattaaa
     1741 aaagaaatat ttttctgtaa atgatttgtt gattattgaa ttgtatttct tttgttgtgc
     1801 tatgggatta gaagataaag aacattttga ggagttagct caaaaagtat tgctatgttc
     1861 agaatatgaa gataaggcta gcctagttca aatggaaaaa gttcttctga gcctatttat
     1921 ccaaattcag acagaagata gtctgatata tattcaaaca tttgagaaaa ttattgctaa
     1981 aacaagacat gttttttacc gacctcatct atttttgcta aaggcaaaat atgctttatt
     2041 tgtagacaag aatattttag aggcagaaag tttctatgaa aaggcgattt ctctagcaga
     2101 attgctagac gatcaggtat tagttcagag aattttagca gaaaaacaga tagatttccc
     2161 aacaacctga cttctaagtc atgttgtttt tttcatgttt ttgctatact aaacctataa
     2221 agaaaacgtt ctcaaaaaga aaggaggttt attatggttg gaacgttatt tgtcctttta
     2281 agtataggaa tttggttcta tctataatat cgggtagaga ttaatggaac aaggtattct
     2341 tatattggat taaaggaatg tcctacatta gtatagagga tgaaactcaa ttttcaatga
     2401 tatagaagag ttatttggag attgtgaggg ttgagtatgt ttatttatga tgtaggaagt
     2461 gtaaactaaa aagaaggtag taatcgtata aaagttaaat agacattatc ttttattgag
     2521 tcttagtgat ataaaagtaa tttgataatc taggaaaatg tagtagtaat ttatttatta
     2581 tagatatgtt aataaaagta gtataaaata tagacgaaca tttgaagaga gttaggaata
     2641 caacatgtgc gaattaactc tcaataaaac atacataata tacaaaagga gtacactaaa
     2701 atgaaatcta aaagaatgga atttcataat aaatttctta ttgtttcagc aatgttagca
     2761 gtcattagtt ggttaagctt aggtgttgtt tcttttccta tgttggctgg gacattagga
     2821 atttcgacaa aagctgctgc tactgtagtc aatttgatta gtgcttatag tacagttaca
     2881 gcagtaattt caattgtagg tgcaatcaca ggagtaggtt ctattggttc gggtattgca
     2941 gcaacagttc tttatatatt gaaaaagaaa ggtgctgcta aagcagctct atggtagaac
     3001 tagatgaaaa tactttctaa aaaagaatgt gaaagtattt taggttatct taattttatt
     3061 gggataaccc ttttgttttt tgttatcata agtttgtcaa tctattttaa aagctatctt
     3121 ccttttcaag ggaaattatt tttacttttt ttggtagtag attacgctac tttgaatcgt
     3181 tataatatga aactgcctat ccaaagtaaa aaaataaata ctttctatat aaggaaaaac
     3241 tatatttttc tctatcttct tgccattgtc aagaaatatt attctttcca aattttacaa
     3301 tcattcattt atttatttat tttgtatttt ctagatatta ttggatattt ttatttatcc
     3361 attactcttt gtttgatagc tattcaaatt atttctttaa ttctacctag tttgttttca
     3421 attataagta aattgttctt atttttatct ataggactat tgtctataga acaaggacaa
     3481 gttgtaattt atgtcatact actcaattat tttattttag tcatggggat attagataat
     3541 catgcgagta atttctattt taaaagcagt cgaatgagtc aaaaaagatt tcgtagcagt
     3601 ctatttatat tgcttcgttt ttgttggtta aataaagggt taactttttc ttttattttg
     3661 attagtattc ttctttccta tctattttgg tatatgatga cgatgttatc aattgatgac
     3721 acaaaaatac caatattatt tctttatacc tcaatatata ttacattaat ggaggcaata
     3781 atagggaatc ataaatcctc aattggggta gatctagctt tacttcaatt atctgctctt
     3841 aatcattcta aaatgaattt cttttttaag aatgaacaac taatgatgtt gacgcattta
     3901 atctctttct tacaattttc attgttgtta tgtttgattc cggtgacaag ttatagtaat
     3961 gatataaaaa gtattttgtt gaatttgtta ttgattccat tcaattattt tatagcctat
     4021 gtctattata agaaagctct atttttaatt aaaggtacag ttagtttatt gagatggagt
     4081 tttctggttt tatattgttt tctgataata tttgtaattt tagtaggggg cattagatga
     4141 gaaaaagtgg ttttctgatt tactatgtgt tattttgtat agtctgtttt ttactaggtt
     4201 tacttttaag ctttatatta aaaaatgaga cttttgctac tatacctccg aaactggaat
     4261 tacaatttac attgacagtt ttacaggcaa attctacaaa ttttttgcta tatctattct
     4321 gttttccgat ttcgccctta ttgcaacttt ttgatttgct atctttagga atacaattgg
     4381 gtatatctgg tcagatgatt ggaatagtag ctacaataaa aggaatatta cctcatgctc
     4441 ttattgaaat accgaatttt atttggtatc aaggtatgag tcagtatctt ttataccgat
     4501 tattgaagga taggggaatt aaaaatatac ttatttatat aaaaagtaat ttaaaatggt
     4561 atatcatttc ttatctttta ttaattttag cagcgttgat agagggaatt aaattatgaa
     4621 aattgaaaat gaatctaaaa gttatgataa atgtatattg aaaaatatat cactagagat
     4681 tgataaggga atttacagag ttgaaggaga gaatggttca ggaaagagca cccttctaca
     4741 acttttagct gggctagaga cttttgatag tggtttaaag aattgtgtct ccaaagatgt
     4801 actatacctt gataccaatc agcttggggt tgttccattt actatagaag aaaatttgca
     4861 gttgttgtgt gattcttttc aaatccatct atcatttgag gataaagaac aaattaatac
     4921 tttttttgat aataaaatcg gagataacta tatgacagca tctactggaa caaaattcaa
     4981 attaggctta tccttaattt ttgctaaaaa ttgggattat atttttattg atgagacttt
     5041 atcaacagtg gatgcaagaa gtattgatat gattgctaaa cgcttaattt ccatgaaaga
     5101 aagctctact attatatatg tatctcataa tttattgaat caagaattga ttagcaaatc
     5161 tcaaaggatt ttaatagagg aaggacgggt ttatgaaatt tcaaacaaat aatagtttat
     5221 acaaattaat gaatctgatt atttatcttt ttcttttata tttgtttatt gatttactga
     5281 tgaagtcggg aattttagat gaacaaattg ataaattatc tgaactatct aatcttttta
     5341 caattaaaat agaaacaata ttgacagtga tttctgttgt tttcacattt atagcaactc
     5401 ttattgaata tggattttct ctctttctga ttacattatt ttttgataag aataagagga
     5461 actttggaga tattctttta tctaaaaata taaccttaag ttttagcatt ttactttgtt
     5521 atttatttaa tccatcggat ataaagtttg cctatttcag cgtattgaca gtattactaa
     5581 ttgggataac aaattatgcg agatttaaaa atttaagatt gacagtagtg ttttctgtac
     5641 catttttatt taatgctata atgtctatta taaatagttt ttagaaatac taaaattttt
     5701 ttctttactt aataatgata tgaaatcatt tcggttttgt taacttgtaa ttcttatatt
     5761 ttctaggtag aacgtttgtt ctgcctattt ttttatccaa aaagtgcagt tgggagggag
     5821 ataggctcat ttgggaagga agtccagttt ttgtttagtg attggggtaa gatagttgtt
     5881 atcagatgag tttatactct tcgaaaatca aattcaaacc acgtcaacgt cgccttgccg
     5941 tatatatgtg actgacttcg tcagtcctat ctacaacctc aaaacagtgt tttgagcagc
     6001 ctgcggctag tttcctagtt tgctctttga ttttcattga gtattaggga aaaggagatg
     6061 aatatgaaat ttgggaaacg tcactatcgt ccgcaagtgg atcagatgga ctgcggtgta
     6121 gcttcattag ccatggtttt tggctactat ggtagttatt attttttggc tcacttgcga
     6181 gaattggcta agacgaccat ggatgggacg acggctttgg gcttggtcaa ggtggcagag
     6241 gagattggtt ttgagacgcg agccattaag gcggatatga cgctttttga cttgccggat
     6301 ttgacttttc cttttgttgc ccatgtgctt aaggaaggga aattgctcca ctactatgtg
     6361 gtgactgggc aggataagga tagcattcat attgccgatc cagatcccgg ggtgaagttg
     6421 actaaactgc cacgtgagcg ttttgaggaa gaatggacag gagtgactct ttttatggca
     6481 cctagtccag actataagcc tcataaggaa caaaaaaatg gtctgctctc ttttatccct
     6541 atattagtga agcagcgtgg cttgattgcc aatatcgttt tggcaacact cttggtaacc
     6601 gtgattaaca ttgtgggttc ttattatctg cagtctatca ttgataccta tgtgccagat
     6661 cagatgcgtt cgacactagg gattatttct attgggctag tcatcgtcta catcctccag
     6721 caaatcttgt cttacgctca ggagtatctc ttgcttgttt tggggcaacg cttgtcgatt
     6781 gacgtgattt tgtcctatat caagcatgtt tttcacctcc ctatgtcctt ctttgcgaca
     6841 cgcaggacag gggagatcgt gtctcgtttt acagatgcta acagtatcat cgatgcgctg
     6901 gcttcgacca tcctttcgat tttcctagat gtgtcaacgg ttgtcattat ttcccttgtt
     6961 ctattttcac aaaataccaa tctctttttc atgactttat tggcgcttcc tatctacaca
     7021 gtgattatct ttgcctttat gaagccgttt gaaaagatga atcgggatac catggaagcc
     7081 aatgcggttc tgtcttcttc tatcattgag gacatcaacg gtattgagac tatcaagtcc
     7141 ttgaccagtg aaagtcagcg ttaccaaaaa attgacaaga aatttgtgga ttatctgaag
     7201 aaatccttta cctatagtcg agcagagagt cagcaaaagg ctctgaaaaa ggttgcccat
     7261 ctcttgctta atgtcggcat tctctggatg ggggctgttc tggtcatgga tggcaagatg
     7321 agtttggggc agttgattac ctataatacc ttgctggttt actttaccaa tcctttggaa
     7381 aatatcatca atctgcaaac caagcttcag acagcgcagg ttgccaataa ccgtctaaat
     7441 gaagtgtatc tagtagcttc tgagtttgag gagaagaaaa cagttgagga tttgagcttg
     7501 atgaagggag atatgacctt caagcaggtt cattacaagt atggctatgg tcgagacgtc
     7561 ttgtcggata tcaatttaac cgttccccaa gggtctaagg tggcttttgt ggggatttca
     7621 gggtcaggta agacgacttt ggccaagatg atggttaatt tttacgaccc aagtcaaggg
     7681 gagattagtc tgggtggtgt caatctcaat cagattgata aaaaagccct gcgccagtac
     7741 atcaactatc tgcctcaaca gccctatgtc tttaacggaa cgattttgga gaatcttctt
     7801 ttgggagcca aggaggggac gacacaggaa gatatcttac gggcggtcga attggcagag
     7861 attcgagagg atatcgagcg catgccactg aattatcaga cagaattgac ttcggatggg
     7921 gcagggattt caggtggtca acgtcagaga atcgctttgg cgcgtgctct cttgacagat
     7981 gcgccggtct tgattttgga tgaggcgact agcagtttgg atattttgac agagaagcgg
     8041 attgtcgata atctcattgc tttggacaag accttgattt tcattgctca ccgcttgact
     8101 attgctgagc ggacagagaa ggtggttgtc ttggatcagg gcaagattgt cgaagaagga
     8161 aagcatgctg atttgcttgc acagggaggc ttttacgccc atttggtcaa tagctagaaa
     8221 gaggagagga tgaaaccaga atttttagaa agtgcggagt tttataatcg tcgttaccat
     8281 aatttttcca gtagtgtgat tgtacccatg gcccttctgc tcgtgttttt acttggcttt
     8341 gcaactgttg cagagaagga gatgagtttg tccactagag ctactgtcga acccagtcgt
     8401 atccttgcaa atatccagtc aactagcaac aatcgtattc ttgtcaatca tttggaagaa
     8461 aataagctgg ttaagaaggg ggatcttttg gttcaatacc aagaaggggc agagggtgtc
     8521 caagcggagt cctatgccag tcagttggac atgctaaagg atcaaaaaaa gcaattggag
     8581 tatctgcaaa agagcctgca agaaggggag aaccactttc cagaggagga taagtttggc
     8641 taccaagcca cctttcgcga ctacatcagt caagcaggca gtcttagggc tagtacatcg
     8701 caacaaaatg agaccatcgc gtcccagaat gcagcagcta gccaaaccca agccgaaatc
     8761 ggcaacctca tcagtcaaac agaggctaaa attcgcgatt accagacagc taagtcagct
     8821 attgaaacag gtacttcctt ggccggtcag aatctagcct actctcttta ccagtcctac
     8881 aagtctcagg gcgaggaaaa tccccaaact aaggttcagg cagttgcaca ggttgaagca
     8941 cagatttctc agttagaatc tagtcttgct acttaccgtg tccagtatgc aggttcaggt
     9001 acccagcaag cctatgcgtc agggttaagc agtcaattgg aatcccttaa atcccaacac
     9061 ttggcaaagg ttggtcagga attgaccctt ctagcccaga aaattttgga ggcagagtca
     9121 ggtaagaagg tacagggaaa tcttttagac aaggggaaag ttacggcgag tgaggatggg
     9181 gtgcttcatc ttaatcctga gaccagtgat tctagcatgg ttgcagaagg tgccctacta
     9241 gcccaacttt atccatcttt ggaaagagaa gggaaagcca aactcacagc ttatctaagt
     9301 tcaaaatatg tagcaagaat caaggtcggt gattctgttc gctatactac gactcatgat
     9361 gccgggaatc aacttttcct agattctact attacaagta ttgatgcgac agctactaag
     9421 actgagaaag ggaatttctt taaaatcgag gcggagacta atctaacttc ggagcaggct
     9481 gaaaaactta ggtacggggt ggaaggccgc ttgcagatga ttacgggcaa gaaaagttac
     9541 ctacgttatt atttggatca atttttgaac aaagagtaat gttcgtgttt ttagagttaa
     9601 ataattttta aactgtgaga aagattcttc ttgcagtttt ttctttacaa tttttgaaaa
     9661 acatctacta tttattcggt taaattcttg tgttttttgg ttttttgtgg taaaatgtgc
     9721 tcaagtaata cgaaaggcga actttaaaat gtcaaaacaa ttgatctatt cgggaaaagc
     9781 taaagatatc tatacaactg aggatgaaaa tcttattatt tcaacttaca aggaccaggc
     9841 gactgctttc aacggtgtca agaaggagca gattgcaggt aagggagtct tgaataatca
     9901 gatctcatct tttatttttg agaaattaaa tgtggctggt gtggcgactc actttgtgga
     9961 gaaactttca gacacggaac aactcaataa aaaggttaag attattcctt tggaagtcgt
    10021 gctccgcaac tatactgctg gttccttttc aaaacgtttt ggtgtggatg agggaatcgc
    10081 cttggagact ccgattgtcg aattttacta caaaaatgat gatttggatg atccatttat
    10141 caatgatgag catgtgaaat tcctacagat tgcgggtgac cagcagattg cctacttgaa
    10201 ggaagaaacg cgtcgtatca atgaactatt gaaagtctgg tttgctgaga ttgggcttaa
    10261 attgattgac tttaagctag agttcggttt tgacaaggat ggcaagatta tcttggcaga
    10321 cgaattttca ccagataact gccgcttgtg ggacgctgat ggcaaccaca tggataagga
    10381 tgttttccgt agaggattgg gagaactaac cgacgtttat gagattgttt gggaaaagtt
    10441 gcaggaattg aaataatctg tttgcaacgg aaaaccttcg tctctcaact aaaaggactc
    10501 aggctgaaaa ggtcccccag accttttcac tctgtagaga actaggtgaa ctaacagatg
    10561 tttacgaaat tgtctgggaa aagttgcagg gtttaaaata acaacctcaa ggctgtttgg
    10621 gaatattgca agagctgaaa taaaggaata agaattgatg gataaacgta tttttgttga
    10681 aaaaaaggct gattttcagg tcaagtcaga gagtttggtt agagagctcc agcacaactt
    10741 gggactgtca agcttgaaaa gtattcgtat tgtgcaagta tatgatgtat ttgacttggc
    10801 tgaggacttg tttgcacctg cagagaagca cattttctct gagcagggaa ccgaccatgt
    10861 tttagatgaa gtatctgtgc aggcggatct tgctaactat gctttctttg ccattgaaag
    10921 tctgccaggg cagtttgacc agcgtgcagc ttcgtcacag gaagccttgc ttttgttggg
    10981 aagttcgagt gacgtgacag tcaacacagc ccaactttac ttggtgaata aagatattga
    11041 tgcgactgag ttggaagctg tcaaaaacta cctgctcaat ccagttgatt ctcgtttcaa
    11101 ggatatcacg acagggattg ccaagcagga gttttcagag tcagacaaga ccattccaaa
    11161 attgactttc tttgaaagct atacagcaga agactttgct cgctacaagg ccgagcaagg
    11221 gatggccatg gaagtggatg atttgctctt tatccaagac tactttaagt caatcgggcg
    11281 cgtgccaact gagactgaac tcaaggtttt ggacacttac tggtctgacc actgccgtca
    11341 tacgactttt gagacagagt tgaaacacat cgacttttca gcttctaaat ttcaaaagca
    11401 attgcagtca acctatgaca agtatattgc catgcgcgag gagttaggtc ggtctgaaaa
    11461 accacaaacc ttgatggata tggcgactat tttcggtcgt tatgagcgtg ctaacggacg
    11521 attggatgat atggaagtct ctgacgaaat caatgcctgc tcagttgaaa ttgaagtgga
    11581 tgttgatggt gtcaaggaac cttggctcct catgtttaaa aatgaaaccc acaaccatcc
    11641 aacagaaatt gagccatttg gtggagcggc tacctgtatc ggtggagcta ttcgtgatcc
    11701 gttgtcaggc cgttcctatg tttaccaagc catgcgtatt tcaggtgctg gtgatattac
    11761 agcaccgatt tcggaaactc gtgctgggaa attgccacaa caagtcattt ctaaaacagc
    11821 agctcatggt tattcttcat atggtaacca gattgggctt gccacaacct acgttcgtga
    11881 atacttccac ccaggctttg tagctaaacg tatggaactt ggtgccgttg ttggtgcggc
    11941 tcccaagggc aatgttgtcc gtgaaaaacc tgaagcaggt gatgtgatca tccttctcgg
    12001 aggcaaaaca ggtcgtgatg gtgtcggtgg tgcgacgggc tcttctaagg ttcaaacagt
    12061 tgagtctgta gagactgctg gtgctgaggt tcaaaaagga aatgccatcg aagaacgcaa
    12121 gattcagcgc ctcttccgta atggcaatgt cactcgtctg atcaagaagt ccaatgactt
    12181 tggggcaggc ggcgtctgtg tggctatcgg tgaattggca gacggtcttg aaatcgacct
    12241 caacaaggtg cctcttaaat accagggctt gaatggtaca gaaattgcca tctctgaatc
    12301 acaagaacgg atggcggtcg tggttcgtcc tgaagatgtg gataccttcg ttgccgaatg
    12361 taacaaagaa aatattgatg ctgttgtggt ggcgacagta actgaaaaac caaatcttgt
    12421 catgcactgg aatggtgaga caatcgttga cttggagcgt cgtttccttg acaccaatgg
    12481 tgtgcgcgtg gttgtcgatg ccaaagttgt ggacaaggat gtcaaactcc cagaagagcg
    12541 tcaaacatct gctgaaacac tggaatcaga tacccttacg gttctatctg acctcaacca
    12601 tgcaagtcaa aaaggattac agactatctt tgactgctct gttggacgct caacggttaa
    12661 tcacccactt ggtggtcgtt accaactcac accaactgag gcatctgtgc agaaattgcc
    12721 agttcaacac ggtgtgactc atactgcgtc ggtcattgct caaggtttca acccatatgt
    12781 agctgaatgg tctccatacc acggtgctgc ttatgcggtt atcgaagcaa ctgctcgttt
    12841 ggtggctgct ggtgccaact ggtctaaggc tcgtttctct taccaagaat atttcgagcg
    12901 gatggacaag caagcagagc gtttcggtca gccagtagct gctcttctag gttctattga
    12961 agcacaaatt cagcttggct tgccatctat cggtggtaag gactccatgt ctggtacctt
    13021 tgaagaattg accgttccgc caaccttggt tgcctttggg gtgacgacgg cagatagccg
    13081 taaggtgctc tctccagaat ttaaagctgt tggggaaaat atctactaca tcccaggtca
    13141 agccctctct gcagagattg attttgactt gattaagaaa aattttgctc agtttgaagc
    13201 aagccaagct gaccata
//
