LOCUS       NZ_FHRS02000012        19997 bp    DNA     linear   CON 12-DEC-2024
DEFINITION  Streptococcus pneumoniae strain GPSC18 substr. ST1797
            ERS455146SCcontig000012, whole genome shotgun sequence.
ACCESSION   NZ_FHRS02000012
VERSION     NZ_FHRS02000012.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2016) WTSI, Wellcome Trust Sanger Institute, CB10
            1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900055005.2-RS_2024_12_12
            Annotation Date                   :: 12/12/2024 02:43:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,241
            CDSs (total)                      :: 2,189
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 73 of 180
            Pseudo Genes (incomplete)         :: 93 of 180
            Pseudo Genes (internal stop)      :: 57 of 180
            Pseudo Genes (multiple problems)  :: 36 of 180
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:31:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 33349
            Orig. end                         :: 53346
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FHRS02000012.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..19997
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..19997
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..19997
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:14997](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..14997)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(613..631)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 25.52"
                     /tool="antismash"
     gene            complement(642..848)
                     /locus_tag="A1898_RS11250"
                     /old_locus_tag="SAMEA2521606_01258"
     CDS             complement(642..848)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680085.1"
                     /locus_tag="A1898_RS11250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01258"
                     /product="hypothetical protein"
                     /protein_id="WP_000680084.1"
                     /transl_table=11
                     /translation="MKHFFAGIGEIKLVGYLLYICVGLAPLFHIYVIGSELSFVKIVLS
                     ILGIIFVSMLTIARIYRTFFYKE"
     gene            complement(1327..2055)
                     /locus_tag="A1898_RS06210"
                     /old_locus_tag="SAMEA2521606_01259"
     CDS             complement(1327..2055)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /locus_tag="A1898_RS06210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01259"
                     /product="hypothetical protein"
                     /protein_id="WP_000072907.1"
                     /transl_table=11
                     /translation="MSNVDKIRKIHIIVCWMYIFLSFRAIINDTEYFLLIFLAFIYSIV
                     SLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLISVFITELIIFHLIG
                     KDFDIKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVDNRVLALIFLI
                     KICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSVLLLF
                     SNFSVPFIKEKEYELFKNSK"
     gene            complement(2052..2405)
                     /locus_tag="A1898_RS06215"
                     /old_locus_tag="SAMEA2521606_01260"
     CDS             complement(2052..2405)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /locus_tag="A1898_RS06215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01260"
                     /product="hypothetical protein"
                     /protein_id="WP_000424408.1"
                     /transl_table=11
                     /translation="MELVLPNNYVALEQEEMMYLDGGAYLSKRACQGICAALAMSPGTF
                     IALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLAGAAGRIAYCIGYGALNRGCDISGN
                     PYPWDGFISATVR"
     gene            complement(2739..3044)
                     /locus_tag="A1898_RS06220"
                     /old_locus_tag="SAMEA2521606_01261"
     CDS             complement(2739..3044)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420335.1"
                     /locus_tag="A1898_RS06220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01261"
                     /product="SPAR17_0115 family bacteriocin-like peptide"
                     /protein_id="WP_000420335.1"
                     /transl_table=11
                     /translation="MELILPNNYVDLEQEEMMYLDGGEPMSISAIVALAITIGGASYST
                     GYVLGERVYYSGYKNSDYQKNKWGIRGAMLAAFGAIGAVVMLGFENRYYALVTGRG"
     misc_feature    3003..3016
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 21.57"
                     /tool="antismash"
     gene            complement(3192..4544)
                     /locus_tag="A1898_RS06225"
                     /old_locus_tag="SAMEA2521606_01262"
     CDS             complement(3192..4544)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865356.1"
                     /locus_tag="A1898_RS06225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01262"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_000781019.1"
                     /transl_table=11
                     /translation="MKLYNKSELRYSRIFFDKRPPAFAFILIISTAIILSGALVGAAYI
                     PKNYIVKANGNSVITGTEFLSAIGSGKVVTLHKSEGDMVNAGDVIISLSSGQEGLQASS
                     LNKQLEKLRAKEAIFQKFEQSLNEKYNHLSNSGEEQEYYGKVEYYLSQLNSENYNNGTQ
                     YSKIQDEYTKLNKITAERNQLDADLQALQNELLQLQQQGDSPSLSDTTSADDKAKLETK
                     ISEITTKIEALKTNITSKNSEIDSQQSNIKDMNRTYNNPTSQAYNIYAQLISELGTARS
                     NNNKSITELEANLGVATGQDKAHSILAPNEGTLHYLVPLKQGMSIQQGQTIAEVSGKEK
                     GYYVEAFVLASDISRVSKGAKADVAITGVNSQKYGTLKGQVRQIDSGTISQETKEGNIS
                     LYKVMIELETLTLKHGSETVVLQKDMPVEVRIVYDKETYLDWILEMLSFKQ"
     gene            complement(4541..5008)
                     /locus_tag="A1898_RS06230"
                     /old_locus_tag="SAMEA2521606_01263"
     CDS             complement(4541..5008)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096140.1"
                     /locus_tag="A1898_RS06230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01263"
                     /product="hypothetical protein"
                     /protein_id="WP_001096141.1"
                     /transl_table=11
                     /translation="MAKLEVKDNKKLVLKSVICKKLHDTKVEDVDQEINKFHQHLQLLK
                     AQIFGPLIVKSCGTTIHDDGLITTDFEFYIQAHNAQQYSNIYDVQDSISVPYCLYVRFE
                     DSPEYLQYAYSKLDLYVYENDIQTDGIVYTVYVNSSPEKMVVDIFRPIVSL"
     gene            complement(5001..7220)
                     /locus_tag="A1898_RS06235"
                     /old_locus_tag="SAMEA2521606_01264"
     CDS             complement(5001..7220)
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856353.1"
                     /locus_tag="A1898_RS06235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01264"
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="WP_001856353.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 3.1e-32, bitscore:
                     103.1, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 8e-171, bitscore:
                     561.7, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTDM
                     YGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIKRN
                     SKFYVADPASGIRKMSSDELGEIYQGITLFMLPNSDFERGKLKGKGLLDLFGRLIFNQK
                     GLISTVILASFVLSIIGILSSLFSKVIMDEVIPYALKNSLYMFLIVFGIVSFLQTLLSA
                     FRQHVLLFLSRKIDIPVLMGYYDHIIHLPYSFFGSRRVGDVLTRFQDAMTIKNVFTSVS
                     ISLVMDIILSVISAVVLWTINQSLFLILVFMVIVNIILIYCFKKPYKKINHEQMEANGL
                     LNSQLIESIRNIDTIKSQHDEEQRLNKIEEKFVHTLEIGYKEGVLQNIQSTISSMTSTM
                     GGLLFMGVGALFIIDGKMTIGDLLVFQTLSQYFTEPIQNLVGLQLTFQEVQVAVSRLQE
                     LMEVDREDIALDYSIRDFTLCDDIEFKDVTFAYGSRPPVIKDFNLRIKQGEKIAFVGES
                     GAGKSTLVRLLLRFINPSEGKIRIGENDLSDLDYGKLRKKISYIPQTIELFTGTIIDNL
                     KIGNPSVTYEDMVRVCRIVGIHDTIQRLQNRYGSFVEEGGQNFSGGEKQRLAIARALLS
                     KADLYIFDEATSNLDSFSEQIIQDLIFNKIIDKTTIVVAHRLSTILRCDKICFLENGTI
                     VEYGTHEELMAKNGKYARMVGLQSVQVNQQIQSQAVLDIEEVTYG"
     gene            7686..8549
                     /locus_tag="A1898_RS06240"
                     /old_locus_tag="SAMEA2521606_01265"
     CDS             7686..8549
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /locus_tag="A1898_RS06240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01265"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001268171.1"
                     /transl_table=11
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPKY
                     ENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQDYL
                     KTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLKILN
                     TILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMCKQIC
                     YTLLEDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETSILSHLKKEVEIY
                     YQAKER"
     gene            complement(8838..>9635)
                     /gene="argH"
                     /locus_tag="A1898_RS06245"
                     /old_locus_tag="SAMEA2521606_01266"
                     /pseudo=""
     CDS             complement(8838..>9635)
                     /GO_function="GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA]"
                     /GO_process="GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /gene="argH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107608.1"
                     /locus_tag="A1898_RS06245"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01266"
                     /product="argininosuccinate lyase"
                     /pseudo=""
                     /transl_table=11
                     /translation="GVVALAGTTFPIDRQLSSDLLEFKQPYTNSLDAVSDRDFILEFLS
                     NASILMMHMSRFCEEMINWCSFEYQFITLSDTFTTGSSIMPQKKNPDMAELIRGKTGRV
                     YGHLFGLLTVMKSLPLAYNKDLQEDKEGMFDTVETILNSLDVLAGMLSSLQVNKEKMQE
                     STEKDFSNATELADYLAGKGLPFREAHEVVGRLVLDSIKSAKNLQDWTLEELQTYHSLI
                     TEDIYVYLQPKTAVQRRNSLGGTGFDQVEYQIAVAKKANEAKK"
     gene            complement(9686..10882)
                     /locus_tag="A1898_RS06250"
                     /old_locus_tag="SAMEA2521606_01267"
     CDS             complement(9686..10882)
                     /EC_number="6.3.4.5"
                     /GO_function="GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1291: argininosuccinate synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031904.1"
                     /locus_tag="A1898_RS06250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01267"
                     /product="argininosuccinate synthase"
                     /protein_id="WP_001810177.1"
                     /transl_table=11
                     /translation="MSKEKVILAYSGGLDTSVAITWLKKDYDVVAVCMDVGEGKDLDFI
                     HDKALKVGAVESYVIDVKDEFATDYVLVALQSHAYYEQKYPLVSALSRPLISKKLVEIA
                     HQTGATTIAHGCTGKGNDQVRFEVSIAALDLNLKVIAPVREWKWSREEEIYYAKENGVP
                     VPADLDNPYSVDQNLWGRANECGILENPWNQAPEEAFGITTSPEQAPDMPEYIEIEFSE
                     GVPVSLNGEVLKLADLIQKLNEIAGKHGVGRIDHVENRLVGIKSREIYECPGAVTLLTA
                     HKEIEDLTLVREVAHFKPIIENELSNLIYNALWFSSATQALIAYIKETQKVVNGTAKVK
                     LYKGSAQVVARKSPNSLYDENLATYTSADTVDQDAAVGFIKLWGLPTKVHSEVQKSAK"
     gene            complement(10899..11705)
                     /locus_tag="A1898_RS06255"
                     /old_locus_tag="SAMEA2521606_01268"
     CDS             complement(10899..11705)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /locus_tag="A1898_RS06255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01268"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_001038474.1"
                     /transl_table=11
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVALN
                     PDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKADLA
                     ISGVSKTDERSKVFDFSTPYYTAKNKLIVKKSDLATYQSVNDLAQKKVGAQKGSIQETM
                     AKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNFEKEQ
                     DDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(11912..12553)
                     /locus_tag="A1898_RS06260"
                     /old_locus_tag="SAMEA2521606_01269"
     CDS             complement(11912..12553)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /locus_tag="A1898_RS06260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01269"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000571329.1"
                     /transl_table=11
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLLN
                     LLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLMLAL
                     KYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLILADE
                     PTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRIIELGEGK"
     gene            complement(12550..14658)
                     /locus_tag="A1898_RS06265"
                     /old_locus_tag="SAMEA2521606_01270"
     CDS             complement(12550..14658)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /locus_tag="A1898_RS06265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01270"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_000724257.1"
                     /transl_table=11
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDLY
                     LKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLDFT
                     TDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEMDKD
                     RIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQPMMEM
                     KRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPMLWLSHF
                     LLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTALLIGVGR
                     SLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFYQDLVEKT
                     QAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTGIKKVILMP
                     ASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFPYSDKRKENL
                     SNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKLSDGNYLKFSS
                     IQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRDIITKKFMGWKL
                     VDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVLGLASRMEKRSLV
                     ELLKGGIL"
     gene            complement(14710..14997)
                     /locus_tag="A1898_RS06270"
                     /old_locus_tag="SAMEA2521606_01271"
     CDS             complement(14710..14997)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /locus_tag="A1898_RS06270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01271"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_001230165.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 3.5e-32,
                     bitscore: 102.8, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFGY
                     SDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
     misc_feature    15043..15059
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 23.57"
                     /tool="antismash"
     misc_feature    15318..15331
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 25.42"
                     /tool="antismash"
     gene            <15418..15744
                     /locus_tag="A1898_RS06275"
                     /pseudo=""
     CDS             <15418..15744
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /locus_tag="A1898_RS06275"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="DNA-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="KILQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYT
                     LLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            complement(15796..16383)
                     /locus_tag="A1898_RS06280"
                     /old_locus_tag="SAMEA2521606_01272"
     CDS             complement(15796..16383)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /locus_tag="A1898_RS06280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01272"
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="WP_000836442.1"
                     /transl_table=11
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLSE
                     IAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETVSA
                     PVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYS
                     AENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     misc_feature    complement(16453..16469)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 26.23"
                     /tool="antismash"
     gene            16625..17296
                     /gene="sdaAB"
                     /locus_tag="A1898_RS06285"
                     /old_locus_tag="SAMEA2521606_01273"
     CDS             16625..17296
                     /EC_number="4.3.1.17"
                     /GO_function="GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /GO_process="GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sdaAB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /locus_tag="A1898_RS06285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01273"
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="WP_000838912.1"
                     /transl_table=11
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPTE
                     VEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKDSN
                     APHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDIPGM
                     IALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHNVNFF
                     K"
     misc_feature    complement(17305..17321)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 24.79"
                     /tool="antismash"
     gene            17305..18177
                     /gene="sdaAA"
                     /locus_tag="A1898_RS06290"
                     /old_locus_tag="SAMEA2521606_01274"
     CDS             17305..18177
                     /EC_number="4.3.1.17"
                     /GO_function="GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /GO_process="GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sdaAA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /locus_tag="A1898_RS06290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01274"
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="WP_000500038.1"
                     /transl_table=11
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLMER
                     NLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNEHN
                     AKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASISGA
                     EGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEVPCVK
                     RNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLATTPTGR
                     RLQKEIFGE"
     gene            18199..18831
                     /locus_tag="A1898_RS06295"
                     /old_locus_tag="SAMEA2521606_01275"
     CDS             18199..18831
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /locus_tag="A1898_RS06295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01275"
                     /product="HAD family hydrolase"
                     /protein_id="WP_000200892.1"
                     /sec_met_domain="HAD_2 (E-value: 3.8e-39, bitscore: 126.5,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIRG
                     FMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPLYI
                     TTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIIIGDT
                     KFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            18881..19093
                     /locus_tag="A1898_RS11255"
                     /old_locus_tag="SAMEA2521606_01276"
     CDS             18881..19093
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A1898_RS11255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMEA2521606_01276"
                     /product="hypothetical protein"
                     /protein_id="WP_000692954.1"
                     /transl_table=11
                     /translation="MKIKEQTRKLAAGCSKHCFEVADRTDEVSSKHCFKVVDGTDEVSS
                     KHCFEVVDRTDEVSNHIRQGDVDVV"
ORIGIN
        1 ccagtttgaa taacagatat cgtatgacta gcaaaatctc gtgtgatttt gtgtaagcca
       61 aataaatgat gtaaatcagt caggcaaaaa ttcactttga aagtctctaa atacttataa
      121 ttcgttgata cttgacatgt ttttctattg aatctttctt gataatccaa aatacaatca
      181 agtaatttca acgacaaact ccatattttc tcaaaataaa aagcacgggg cagttctcac
      241 tactgcccaa agtgtttgat tagagtcggc ttctctaact ccctatgttg tgcttattag
      301 gcttatcgca agggaacagt ttttagctct gccaagccac atagtgtgct tactactatg
      361 tgtccatgtc accatgaatt gccgaacggt ttaacgagaa agtatagtag attgaaacaa
      421 gatgtgaaca aatcgattag gaaagtcaaa ttaatttcta gaaatatttt aacggtcata
      481 acggactatt ccagattcaa tctactatat tatttttagg tttatcccaa ctcaattatg
      541 acatcttttt ttcaaaagtc aatatatctc actcttttca acgacaagaa agaggctgat
      601 aatcgaccaa tctctgtttt tagatacagt tcaagtccaa actattcttt atagaaaaaa
      661 gtcctataaa ttcgagcaat agttaacata cttacaaata taattcccaa aatagataac
      721 actattttca caaaactcag ttccgaacct atgacatata tatgaaaaag aggggctagt
      781 cctacacata tatacaataa gtagccaaca agttttatct caccaattcc tgcaaaaaaa
      841 tgtttcatgt aacgattctc catttactcc attaaatcag ttttatttta gactatcata
      901 aaacaatcac ctcttcaata aaaaccattg gcgaaatact attctgaagc attgatagct
      961 cagatttctc agaataaagc tgtcctaact atcgagcagc tcaaatacat atatatgagc
     1021 aattctaagc ttagaattgt tataaaataa atgttttcgg gctattctaa gtctttgact
     1081 gcttaaaatt ttacgttctg aaggattcta aagctagaat agctcaaaat cacgagaact
     1141 aaggtattcg aaacttcaga gtaatacaat ccccatagga attctccatt cttattaagg
     1201 atgattaaaa tgtaatccta acaagtgatc ctcgactgtg tttgtagcac ttagttacct
     1261 agataagact atttcccttt atcatatctg attttaagcc gttctaagat ttaacctatg
     1321 tttctattac ttgctatttt taaataattc gtattccttt tctttgataa atggaactga
     1381 aaaattagaa aaaagaagta aaaccgaaaa caaatagcct gctttttgaa tcactaaaag
     1441 atatcctaaa aataagtaag ctataacatc caatgcgaaa agaaaaaata tcctcctctt
     1501 cattaaagta cttgtgtcaa ataatctgat gaataaaaat tttatcgtag tatctacaca
     1561 aatttttatc aaaaagataa gagccaatac acgattatca actgtaccat aaagaataga
     1621 tggaaatata gttaatataa aactagaaat ctctaaatat tttatccaag gagattgaga
     1681 caccttgctt ctaaactgac taattttctt atattcgtta gtcaatttaa tatcaaaatc
     1741 tttcccaatt aaatgaaaaa ttatcaactc tgtaataaaa acacttatta atacggtata
     1801 agtcaaaaaa ctttctggga aaaacttatt gattaaaatg gggaaactca ttaacagaat
     1861 cgaatttatg gctagacaga tagatactat tttatttttc acagaatata gaggtagtga
     1921 tactattgag tagataaaag ctaaaaaaat aagcaaaaaa tactccgtgt catttataat
     1981 cgccctgaat gataaaaata tatacatcca acatacgata atgtgtattt ttcgaatctt
     2041 atctacatta ctcatcttac tgtcgcagat atgaatccat cccaaggata agggttcccg
     2101 ctaatatcac aacctctatt aagagcacca tatccaatac agtaagctat tcttcctgcc
     2161 gccccagcca atacacctgc tgctgcacca ataagccaac ctccaaggcc tccaacttta
     2221 atatagttta ttagtttttt ggttaaaact gcagctccag ctaatgctat aaaagttcct
     2281 ggactcatag ctaaagctgc gcaaattcct tgacaagcac gcttgcttaa ataagctccc
     2341 ccatcaagat acatcatctc ttcttgctca agagcaacat aattatttgg taatacgagt
     2401 tccatttatt ttctcctttt ttattaagca tggttaggat agtttcctaa caagtgatcg
     2461 caggctttgt ttagttacct aaattaaact tccttccttt atcttatttt aaatatttaa
     2521 aaccagttac tgcttgaagc ttaacagttt acttaattca aataacagct aatcactgat
     2581 tccctaattt tatcaaatat actaataaca acaattgtga tacgatcatc attataagaa
     2641 caacatttaa cgaatcaaag tttttccgag ataataataa agcaacagca agcagtatta
     2701 ttactaaaga ttgaataact actattactc ttaacatttt atcctctacc agtaaccaaa
     2761 gcatagtatc tattttcaaa accgagcata actactgcac ctattgctcc aaatgcagcc
     2821 agcattgctc ccctaatacc ccacttattt ttttgataat cagagttttt atatcctgaa
     2881 tagtagactc tttctcctaa cacatatcct gtgctatacg atgcgccacc aattgtgata
     2941 gccaaggcaa caatggccga aattgacatt ggttctccgc catcaagata catcatctct
     3001 tcttgttcaa gatcaacata attatttggc aatatgagtt ccatttattt tctccttttt
     3061 tattaagcat ggttaggata gtttcctaac aagtaatcgt cgactgtgtt tctatcactt
     3121 agctacctag ataagatatc ctttctctac cttatttatt tttataggtt acctaagggg
     3181 tttaaaacca attattgctt gaaacttaac atttctaaaa tccaatcaag ataggtttct
     3241 ttatcataga caatccgcac ttcaactggc atatcctttt ggaggacgac tgtctcactt
     3301 ccatgtttta gagttaaggt ttctaattct atcatgactt tatagaggct aatattcccc
     3361 tctttcgttt cttgggaaat tgttcctgaa tcaatctgtc tgacttgtcc ctttagtgtt
     3421 ccatattttt gactatttac accagtaata gcaacatcag cttttgctcc tttcgaaaca
     3481 cgagaaatat cactcgcaag tacaaaagct tctacatagt aacctttttc tttccctgaa
     3541 acttctgcta tcgtttgccc ctgctgaata gacattcctt gtttcaaagg taccagataa
     3601 tgcagagtac cttcatttgg cgctaatata ctatgtgctt tatcttgacc tgttgccact
     3661 ccaagattag cctcaagctc tgtaatactt ttattgttgt ttgaacgagc agtacctaac
     3721 tcactaatta attgagcata aatattataa gcctgagaag ttggattatt ataggtacgg
     3781 ttcatatcct taatattgct ttgttgacta tcaatctcgc tattcttaga agtaatattt
     3841 gtttttaatg cttcgatttt tgttgttatt tctgatatct tagtttctaa cttagcttta
     3901 tcatcagctg atgtcgtatc tgataaagaa ggtgagtctc cttgctgttg aagttgaagc
     3961 aattcatttt gtagagcttg caagtcggca tctaactgat ttctttcagc tgttatttta
     4021 ttcaacttcg tatattcatc ctgaatcttt gaatactggg taccattatt ataattttcc
     4081 gagtttaact gagacagata gtattcaact ttcccataat attcctgttc ttcaccagaa
     4141 ttagataaat gattgtattt ctcatttaat gattgttcaa atttttgaaa gatagcttct
     4201 tttgcacgta acttctccaa ttgtttattt aaagagctcg cttgtaaacc ttcttgtcca
     4261 cttgataacg aaataatgac atctccagca tttaccatat ctccttctga cttatgtaaa
     4321 gtaactacct tccctgaacc aattgctgat aggaactctg tacctgttat aactgaattt
     4381 ccattcgctt ttacaatata gtttttgggt atataagctg cgccaaccaa tgcaccgctt
     4441 aagataatag cagttgaaat aatgagaata aacgcaaaag ctggtggtct cttatcaaag
     4501 aaaatacgag aataacgtaa ttctgattta ttatataatt tcataggctt acaattggtc
     4561 taaaaatatc tactaccatt ttttcaggag aagaattaac ataaactgta tagacaatcc
     4621 catccgtttg aatatcattt tcatagacat atagatccaa tttagaatac gcatactgta
     4681 gatactctgg actgtcttca aaacgaacat ataaacaata tggaacagag atagaatcct
     4741 gtacatcata aatgttactg tactgttgag cattatgagc ttgaatataa aactcaaaat
     4801 cagtcgttat taatccatca tcatgaatag tagtaccaca actttttaca attaatggac
     4861 caaaaatttg tgcttttaac aactgcaaat gttgatgaaa tttattaatt tcctgatcta
     4921 catcttctac ttttgtatca tgtaactttt tacagataac tgactttagt accagttttt
     4981 tattatcttt tacctctaac ttagccataa gtaacctcct ctatatctaa cacagcctgt
     5041 gactgaattt gttgattcac ttgaacgctc tgcaaaccaa ccattctagc atactttcca
     5101 tttttcgcca ttagttcttc atgagtccca tattccacaa tagtcccatt ttctaaaaag
     5161 catatcttat cacatcgaag aattgtagac agcctgtggg ctactacaat tgttgtctta
     5221 tctattattt tattaaagat taaatcctga ataatctgtt cactaaatga atctaagtta
     5281 gaggttgcct catcaaatat atacaaatca gctttactca gtagtgctct tgcaatagcc
     5341 aatcgttgtt tctctccacc cgagaaattt tgaccgcctt cttctacaaa ggagccataa
     5401 cgattttgaa gacgttgaat cgtatcatga ataccaacaa tacgacaaac tctcaccata
     5461 tcctcatatg taacagaagg attaccaatt tttagattat caataattgt ccctgtaaag
     5521 agttctatgg tctgaggaat gtaggaaatt ttcttacgta gtttaccata gtcaagatct
     5581 gataagtcat tttctcctat acgaattttc ccctcactcg gatttataaa tcttaataac
     5641 aaacgaacta gcgtactttt tcctgctccg ctttctccaa caaaagctat tttttctcct
     5701 tgtttaattc gcaaattaaa gtccttgata acaggagggc gtgaaccata ggcaaatgtt
     5761 acatctttaa attcaatgtc atcacataag gtgaagtctc taatactata atccaaggca
     5821 atgtcttcac gatctacttc catcaattct tgaagccgac taaccgcaac ctgtacctct
     5881 tgaaaggtta actgcaagcc aactaaattc tgaataggtt ctgtaaaata ttgacttaat
     5941 gtctgaaaaa ccaagaggtc cccaatagtc atttttccat caatgataaa taaggcgccc
     6001 actcccataa ataataaccc acccatggtt gaggtcattg aagatattgt agattgaata
     6061 ttctgcaaaa caccctcttt ataaccaatt tctaaagtat gaacaaattt ttcttcaatt
     6121 ttgtttaaac gttgttcctc atcatgctga gacttaatgg tatcaatgtt acgaatagat
     6181 tcaattaact gtgaattaag aagtccattt gcttccattt gttcatgatt aattttttta
     6241 taaggctttt taaaacagta tataagaatg atgttaacaa taaccataaa aactaatatc
     6301 agaaataaag attgattaat agtccaaagg acaaccgcac taataactga caagatgata
     6361 tccatgacta atgaaataga gactgacgta aacacattct ttatcgtcat cgcatcttga
     6421 aatcttgtta aaacatcacc aactctacga cttccaaaaa aactataagg aagatgaata
     6481 atatggtcat aatatcccat taaaaccggt atatcaattt ttcgagatag aaacaaaaga
     6541 acgtgctgac gaaatgcaga tagaagagtc tgtaagaacg acactattcc aaatactatc
     6601 aaaaacatat acagactatt ttttaaagca tatggaatca cctcgtccat gataactttt
     6661 gaaaagagac tcgataaaat accaatgata cttaaaacaa aggaagctaa tatgactgta
     6721 ctaatcaatc ctttttgatt aaaaattaac cttccaaata aatcaagaag tccttttccc
     6781 tttagtttcc ctctttcaaa atccgaattt ggaagcataa aaagagtaat accttgataa
     6841 atttctccca gttcatcact tgacatcttg cgtatgccac tagcagggtc tgctacgtaa
     6901 aatttactat tccttttgat actatgtaat accacaaaat gattttgacc taaatcattc
     6961 ttaacttgaa gaatcgcagg aaatgttaat tttggagtca aatcttccag tgctactcga
     7021 acagctttta ctgtaaaatt caacttattc agacctgaaa caataccact gacagtcgtt
     7081 ccatacatgt ctgtaccaat aatttcccta atcttcatga ttgatagttc ctttttgtaa
     7141 gataaaagaa ctgttgaaac aactgccgca gcacaatctg actggtcatg ttgcatcgta
     7201 caaggatatt tttttaacat agaaatactc aattctttag aattcatcta acttttcttt
     7261 gatgtcaccc aaacctcaat cactgcccag tagttcttga attaatttat tttcaaccgt
     7321 atcattcttt gttcttgata tctttccaag cattgtagta cagcagattt ttgcccaagg
     7381 actgagcctc ctttaacaac tcaaactccc ttacattata tcggccagcc ccatggaaag
     7441 attatagcca gactaatcaa tgatataata catactagaa tacaaaatat ttttttattc
     7501 atactttttc tcctatcttt attttctacc tacatagtac ctcttattaa gctcatttta
     7561 aaattacgtt aacttttttc acaaatttat ttcgactttc ttttttactt tttacattgc
     7621 tatgatacaa tatttttaac aaaattttat tgtttgttaa cttttttcac gaaaaggaga
     7681 aaatcatgcg ttatgatttc ggaaaggtct ataaagaaat acgtgagtca aaggggctga
     7741 cccaagaaga ggtctgtggg ggtgttctct caagaaccag cttatcaaaa atcgaaagtg
     7801 gcaagacaac cccaaaatat gaaaatatgg aatttcttct ccgtcaaatc aatatgagtt
     7861 ttgaagagtt tgaatatatc tgtcaacttt atcaaccaag tcaacgcaca gagattatgc
     7921 aaacctatct caatatgcgt tccattatcg ggaccagcga tcttgtcaat ttatttcaaa
     7981 aatgccaaga ctacctcaag acccaccacg atctccctat agaagaaatc agagatatgc
     8041 ttgaagttgt tatttatctc cgtcaacacg gggcagggga actatcaaaa cacgcagaac
     8101 aggttgtcaa aaaactttgg aaaaaaattg aaaaacaaga tacatggtat gagagtgatc
     8161 taaaaatcct caataccatt cttttcagtt ttcccattga atacctccat ctcatcactg
     8221 gaaaaatatt gcaacgccta gaagtctata aaaactatca acatttatat gacttgcgaa
     8281 tgacaattct attaaacctt tccacactct atctatacaa tcaagataaa aacatgtgta
     8341 agcaaatctg ctacacttta ctggaggacg ccaagaataa gaaaagctac gataggctgg
     8401 ctatctgcta tgtccgtatt gggatttgta gggatgatgc gaaacttatc caaaaagggt
     8461 tctcccttct ggagctaacc gaggaaactt ctatactgtc tcatctcaaa aaagaagtag
     8521 agatctatta ccaagcgaag gaaagataaa aaagccgagg gatttcctcg actctttaca
     8581 tattatttgt tcgttagttc ttaataccag ccattgttaa gccagaagtt tttagctctg
     8641 ccaagccaca tagtgtgttt actactatgt gtccatgtca ccatgaatcg ccgaacgatt
     8701 taacgagaaa gtatgacttt tacgtttatc ccaactcaat tatgacattt ttttcaaaag
     8761 tcaatatatc tcactttttc aacgacaaga aagaggctgc caatcgacca acctcttatt
     8821 ctgaacccat cactccatca ctttttagct tcattcgctt tcttagcgac tgcaatctgg
     8881 tattcgactt ggtcaaaccc tgtgcctccc aaggaatttc gtctttgaac agcagtttta
     8941 ggttgcaagt agacatagat atcctcggta atgagagaat gataggtttg taattcctcc
     9001 aaagtccaat cttgaagatt tttagcagac ttgatagagt ctagcactaa tcttcccaca
     9061 acctcatgag cttctctaaa tggcaagcct ttccctgcca aataatctgc caactcagtt
     9121 gcatttgaaa agtccttctc tgttgattct tgcatttttt ccttgtttac ctgcaaacta
     9181 gataacatac ctgccaacac atcgagggaa tttaaaatcg tttcgaccgt atcaaacatt
     9241 ccttccttat cttcttgcaa atccttatta taagctaaag gcaaggactt catgactgtc
     9301 aacagtccaa acaaatgccc ataaactctg ccagtctttc ctcgaatcaa ctcagccata
     9361 tctggatttt tcttttgggg cataatagag gaacctgttg taaacgtatc tgacaaggta
     9421 atgaattggt actcaaaact acaccaattg atcatttctt cgcaaaaacg actcatatgc
     9481 atcatgagta tgctggcatt tgatagaaat tctaagataa aatcgcggtc actaacagca
     9541 tccaaagaat ttgtataagg ttgcttaaac tccagcaaat cgctagacaa ttggcgatca
     9601 atggggaaag tcgttcctgc caaagcgacc accccataat tttgtattct tagccatatc
     9661 acatccacct ctctatccaa tcgtcctatt tggcgctttt ttgaacctca gaatgaacct
     9721 tggttggaag tccccataac ttaataaagc caacagccgc atcttggtca acggtatctg
     9781 cactagtata cgtcgccaaa ttttcatcgt aaagagaatt tggtgatttc cgagccacga
     9841 cctgagcgct ccccttatac agtttgactt ttgcagtccc attgacaact ttttgagtct
     9901 ctttaatata agcaatcaag gcttgtgtag ccgaactaaa ccacaaagca ttataaatga
     9961 gatttgataa ttcattttct atgattggtt taaaatgagc cacttctctc acaagcgtca
    10021 agtcctcaat ttccttatga gctgtcagca gggtcactgc acctggacac tcatagattt
    10081 ctcttgattt aatcccaacc aagcgatttt ccacgtggtc aatccgtcca accccatgtt
    10141 ttccagctat ttcattgagt ttttgaatca aatctgccaa ttttaacact tctccattga
    10201 gggaaactgg aacaccttca ctaaactcaa tctcaatgta ttctggcata tctggagctt
    10261 gctctggaga agttgtgatg ccaaaggctt cttctggagc ctgattccat ggattttcca
    10321 aaataccaca ctcattggca cgtccccaaa gattttggtc gacagagtag ggattgtcaa
    10381 ggtcagcagg aacaggcacc ccattttcct tggcataata aatttcttcc tcccgagacc
    10441 atttccattc acgaactgga gcaatcactt ttaaattgag atccaaggct gcaatcgata
    10501 cttcaaaacg tacttggtca ttccccttac ctgtacaacc atgagcaatt gtagtcgctc
    10561 ctgtctgatg cgctatttca accaattttt tagaaataag agggcggctc aaggcagata
    10621 ccaaggggta cttttgttca tagtaggcat gtgactggag agctactagc acataatctg
    10681 tagcaaattc gtccttaaca tcaatgacat aagattcgac tgccccaacc ttaagagcct
    10741 tatcatggat gaaatctaag tctttccctt cacccacatc catacaaact gcaacaacat
    10801 catagtcttt cttcagccag gtaatagcaa ctgatgtgtc taaaccgcca gaataggcca
    10861 aaattacttt ttctttactc atgttctttt ccttctttct atttttcaat ggacgcttta
    10921 aaggcatcct caatgagttt gtctaattcc ccagactcct tcaacttttg aatggtttta
    10981 tcgactgcct ctttcaattc cttgctatct tttttcatgg ctaccgcata ggaatcatct
    11041 tgctcttttt caaaattgag gtctgcgatt gctaaatcag gattattttc cacaaatccc
    11101 ttggcaacgg gttcttcaaa aataacggca tccacttgcc ctgattttaa atctgtgatt
    11161 aaattcccat ttttaggcag agatacgagg gaagaatttt gtagcaaatc tttcgccatc
    11221 gtctcttgaa tcgaaccttt ctgcgctccg acctttttct gcgccaaatc gttgacagac
    11281 tgataagtgg ctaaatcaga ttttttgaca atgagtttat tttttgcagt atagtaggga
    11341 gttgaaaaat caaacacctt gctccgttca tctgtcttag aaacacctga tatggcaagg
    11401 tcagcttttc ctgattgaac gctagccagt acattgtcaa aactcatagg agatagttcc
    11461 aattcgacac ctagttctgt tgcgatagcc ttggctaatt cgatatctga acccacaatc
    11521 tgatttttcc catcaaccac tttttgatat tcaaatggag caaaatctgg attgagggct
    11581 acaaccaatt ttcctttgga cttgatggct tcaattccag ccgattgatt gttactacaa
    11641 gcaaatagta gggggagcat cactaaacca aacatcgtca tcaacacctt cttcatctta
    11701 ttcataacaa agcctccttt atgtttattc ttttttattg tataaataat actcctatac
    11761 tcatcctttg tcaagcctaa attgtatttt ttttcaattt aaaacagaaa aacccaggaa
    11821 aatgacataa aaatatcatt cctaggccta tttatgctat ttctctctga aaaatatgag
    11881 tattcagtcg gtcaaatgaa gctgaacgaa ctcattttcc ctcgcctaat tcaatgattc
    11941 gatgacattg ttgggctaca taagcatcgt gggtcacgat aatgactgtt ttcccctctc
    12001 gattcatctc taagagaaac ttcaagacca aatctctatt ttcaggatcc agcgaacctg
    12061 taggttcatc ggctaaaatc agctggctgg gttttaagat ggctctagca actgcaattc
    12121 gttgttgttc gcccccagac aactcggaga ccctttgatg caaagtagct gacaaaccta
    12181 ctctctctaa aatctcttcc acctttttga gcttgtcttt cttaggcaat ttcacatatt
    12241 tcagcgccag catgagattg tactcgaccg tttcatcatc aatcagggca aaattttgaa
    12301 acagataaga gatatgttca cggattattg tttgcgactt agcagaatta accgctagat
    12361 ttgtctgacc aaaaatctca taccgtccgc tataatcacc atctatcaaa cccaataaat
    12421 ttaacaaggt cgacttccca ctaccactct taccaacaat agctaccaaa tccccctgat
    12481 caatcctgag agataagtta tccaaaatca cttttccccc aatggttttg gtaatatttt
    12541 tcaactcaat cataagatgc cccctttcaa taactctact agacttcttt tctccatcct
    12601 agaagctaag cctagcacaa atagtatatc cagacatgta aaacctgcaa acagtagaag
    12661 tggtaagaac gcatgggcaa agaaaatcaa gactagaaga gggaaactat agcccagcaa
    12721 gagcagaacg aggagaggac ggtagcgatc gaccagtttc caccccataa acttcttggt
    12781 aatgatatcc ctgcgcttca ataagaaagt tgttactagt aagaagtagg aaatcatcat
    12841 gctaaggaga ccaaacaaag caaagagtag gttaaaattc cgaacagcat ctcgataaga
    12901 atccactttc tcttgttgaa tggcttgaat agatgaaaat tttaaataat ttccatctga
    12961 caatttctca actaactctg taatctcttt ttgatgttga accgtatttt caattttaat
    13021 cggattattt aagccagttg ttgacaggga ggctttctca tcccacatca tatcagaatc
    13081 attgaccaag ctaataattg gattggagag attttccttt cgcttatcac tatatgggaa
    13141 aaatgaccaa tctccttcat aataggcaat ctcgacatcc atctcctcta tcgttcgttt
    13201 ttgctgctct tcatacttca tcgaatgaaa ggcaattaac ttccccaaga gctgattttt
    13261 atcttcttca cctttcgtac ttgctggcat caaaataact tttttaatac cggtatctgg
    13321 tagcttgaat cccttgctct ttagaaaatt gcgattggca tagtaaacat ccaccgtatc
    13381 tgttaactga tattgctgaa tctgttctga ttggacaaaa ttttttacag gaagactgct
    13441 actctgcaca tagcccgcct gcgttttttc taccaaatcc tgataaaatc gatagaaata
    13501 atctgtagat ttccctgacc ctgctagctc ttcttgccac agattatcat tgagtttgaa
    13561 ggtttctaag gtcaggtaat taccttgact tacccactgt tgctgataag caagttcttt
    13621 gttttcttgt tctaaacttc tgcccacccc aatcagtaag gccgtcagta aaatagttgt
    13681 ccctattttc atcacataat tgaagataag accaaatttg aaagatgaaa aacctttcag
    13741 cagagagctg attgtcattt tttggattaa gaggtaagtc aaccaactga taaagagata
    13801 aagctgcaac agcaaaaaat gagacaacca cagcatagga aacaaatctt ttggcttata
    13861 atcaagcaag aaaaacacgc ctagattgat cacaagagcc ccacctagga ggaggtaaag
    13921 gttgcctttt acaacatcag ctaaaacagc cctatcttga aaaccaagta atttttgtac
    13981 cccaactctt ttcatctcca tcatcggttg atacactgtc actaacacaa gaagcaaaat
    14041 agccaagaca aaaacaatgg cagataaaag caaatctcga tttatgactt ccactgcact
    14101 tttgtaggtc ggctctagca aggtagcctg gtctatcttg aaaaaatcgc tccatttctg
    14161 tacaatccta tccttgtcca tctcttgtgt agaagttatc gtatagcgac catttaaact
    14221 acgagatgta tccttgatat aggtttgaaa agtcataagc tgaataggtt tggcttttag
    14281 aaaggtcgga atcgtaccaa gtttattgga aatttcttta ttactataga ctccttcacc
    14341 atctgtggta aaatcaagag aagaaatccc aaactcttgg taggggaagg tatctttatc
    14401 aaaaacacca gacttgacca cctcatcacc actgtctgtt ttgatgatgg agactttata
    14461 ctcctttgat acatcctcaa aaaatcgaag aacagacgct gcaggttcgt taatatcttt
    14521 caaatacaaa tccaaagaat ctacagtctt tcccatttct tgaatccgaa ccacttctcg
    14581 tgtatagttt acattaaaaa caaaaaaagt ggtacacaga agcaggagta acatacagaa
    14641 attactgatt tttttcataa aagtgcctcc actacagttt tccctctcca aataggagag
    14701 ggaagaattt tactcaaatc catagaaata ttccattcct gttggcggaa tcttggtgag
    14761 ggaagctcta gcccatgcct caggttttgc ataatcctta gaggttctac ccccatgtct
    14821 aacagttgct gtatgatatc gagtagaatg taagtaatca gaatatccaa aagttcctgt
    14881 ccaacctact ccatagttcc attgaccacc ctcaacccat actgctaaag catttgtagt
    14941 agcaagtata ccacatacca ttaaagatgc aacaccaatt ttaactaatt ttctcatgat
    15001 ttatttctcc tattatcgag agctgttccg taaaaacagc tatagtaatt tatcaacaag
    15061 tatcgtagtc cattggactc accttccttc tatctcattt cattttttaa caacgaactt
    15121 actcttaacc gggagaatgg tgcgcttctg tatcaggtcc ttgaacaacc agagtagtat
    15181 taagcacact ctaactatta ctcaagaatc agtccaataa acaatatgga tagcgctttc
    15241 aaatttttaa actcttatcc catcctttta tctatataat aagtgaaaat ataataactg
    15301 tcaagtaatc gaagtgaatt ttataaaaaa aaattacaag ccaaatttgc aaagtttaca
    15361 ataagccgct agggcaatcg tctatcagaa tgtccgttta tttgtcaata atccgagaaa
    15421 atcttgcaac gcttagaagt ctataaaaac tatcaacatt tatatgactt gcgaatagca
    15481 atcctgctca acctttccac actatatcta tacaatcaag ataaaaacat gtgtaagcaa
    15541 atctgctaca ctttactgga ggacgccaag aataagaaaa gctacgatag gctggctatc
    15601 tgctatgtcc gcattgggat ttgtacagac gattctaaac ttatccaaaa aggtttctca
    15661 ctcctagagc tgaccgagga aacttctatg ctgtctcatc tcaaaaaaga agtagagatc
    15721 tattaccaag cgaaggaaag ataaaaaagc cgagggattt cctcgactct ttacatatta
    15781 tttgttcgtt agttcttaat accagccatt gttaagccag aagtttttag cagcagtcca
    15841 tgaaccgtaa cgtcctgcaa cgtaggcatc tgctacacgt tcttggtttt cagctgagta
    15901 gtcaccgttc aggtatgaat ctgttaattg gtaacgtccg atataacgtc catttgtagc
    15961 tgtatagcta ccacctgatt ctttttgagc gatccattct ttggcttctg cttcagatcc
    16021 gcttacagtt gaaacaactg tttcacttac tactggagtt tctgctactg gagctgaaac
    16081 agtttcatct tgagcggctg gtgccgcata agtagctggc gctggtgttg caacaggcgc
    16141 tacagggcca tcgataacca actcttgatc aacataaatc aaatgaatgt tatcaatgtg
    16201 gttgttttct gccaattttt caactgttgt gttgtgagtt tcagcgattt ctgaaagtgt
    16261 atcaccttct ttaacagtgt aagttgatga ttcttgagca gatacaaatg atggagcaaa
    16321 tactgcaaac aaggcagcta ctcctgcaag agttgcttta atctttttag ttattgattt
    16381 catattcgaa aattctcctt ctttctatag ttctatcata ccctttaaat attaccgttt
    16441 cttgacactt ttatgtagaa atattacaaa attatttttt atttccctaa ataaggtgtt
    16501 ttttgtcata aaagatacta tttttatgct atttgaggta ttagaaaggt tttcatccac
    16561 aattaaagcc aagcccctca ttctttgata taatagagat aagaattttt ataaggggaa
    16621 actgatgaaa tcacttcgtt ttcaatctgt ctttgatatc atcggaccag ttatgattgg
    16681 cccatctagt agccataccg ctggtgctgt tcgtattggg aagattgtct cttccatttt
    16741 tgatgatact ccgacagaag ttgaattcca actttttaac tcatttgcca agacctatcg
    16801 tggtcacgga acagacctag cccttgttgc aggtatttta ggaatggata cagatgatcc
    16861 tgaaattcca aatagtctgg aaattgccca caagcgtggt atcaagattg tctggaccat
    16921 tcagaaagac agcaatgccc ctcaccctaa caccactaaa attactgtta aaaatgccca
    16981 caagaccatc agcgtgactg gtatttctat cggtggagga aatattcaag taaccgaact
    17041 caatggtttt gccgtctctc tcaatatgaa tacaccgact atcatcatcg ttcatcaaga
    17101 tattccaggt atgattgccc ttgttacaga ggcgctttcc cgctatggta tcaatatcgc
    17161 ccagatgaat gtcactcgtg aaaaagctgg tgaaaaagcc attatgatta tcgaagttga
    17221 cagtcgcaac tgtgatgagg ctatcgaaga aattcgaaaa atccctcatc tccacaatgt
    17281 caatttcttt aaataggagg aagcatgttt tattctatca aagaattggt cgagcaagca
    17341 gatctggact ttcaaggaaa tgtcgcagaa ctcatgatta caacagagtt tgaattgacc
    17401 ggtcgcgaac gtgaagaagt cttccttctc atggaacgca atctggaagt tatgaaagcc
    17461 tctgtccaac ttggcctcaa tgaaaataaa tctcgtagtg gcctgacagg tggagatgct
    17521 gccaaattgg atcactacat cgaaaacgga aaaactctgt cagattatac gattctctcg
    17581 gctgcccgaa atgccatcgc agtcaatgaa cacaatgcca aaatgggctt ggtctgtgcc
    17641 actccaaccg ctggaagtgc tggctgtctg ccatccgttc tcactgctgc tattgaaaaa
    17701 ttagacctca gccacgagca acaattggat ttcctctttg ctgctggtgc ctttggacta
    17761 gtcatcgcaa acaatgcctc catctcaggt gctgagggtg ggtgtcaagc tgaagttggt
    17821 tcagcctctg ctatgagtgc tgccgccttg actctggctg caggtggaac accttatcag
    17881 gccagtcaag ctattgcctt tgtcattaaa aatatgctag gcctcatctg tgaccctgtt
    17941 gcaggtttgg tcgaagttcc ctgtgtcaaa cgtaatgcca tgggagctag ctttgctttc
    18001 atcgcagcag acatggcctt ggcaggtatc gaatctaaaa tccctgtgga tgaagtgatc
    18061 gatgccatgt accaagtagg agcaagcatg ccaactgcct ttcgtgaaac agctgaaggt
    18121 ggactcgcta ccacccctac tggtcgtcgc ctccaaaaag aaattttcgg agaataagct
    18181 tatcaaatag gagaaaccat gacctctatc acagcgattt ttttcgattt ggatggaacc
    18241 ctcgttgata gttctatcgg gattcacaat gcctttacct atacctttaa agagctgggg
    18301 gtgcctagcc ctgatgccaa aactattcgt ggttttatgg gaccacctct cgaaagtagt
    18361 tttgcgacct gcctgtccaa agaccaaatt tctgaagccg tgcagatata tcgttcttac
    18421 tataaggcaa aaggcatcta tgaagctcaa ctctttcctc agattataga cttgcttgag
    18481 gagttatcga gcagttatcc actctacatc accacgacaa aggatacatc tactgctcaa
    18541 gacatggcaa aaaacttgga aatccatcat ttctttgatg gcatttatgg ttctagccct
    18601 gaagcacccc ataaggcaga tgtcattcac caagccttgc agacacatca actagcacca
    18661 gaacaagcca tcatcatcgg agataccaag tttgatatgc tgggagctcg agaaacaggt
    18721 attcagaaat tggccatcac ttggggattt ggagagcaag cagatctact aaactatcaa
    18781 cctgattata tcgctcacaa acccttagaa gttttggcgt attttcaata gcatagactg
    18841 ggtaacaatt ccatttcaga agaaaatgaa gtaatactca atgaaaatca aagagcaaac
    18901 taggaaacta gctgcgggct gctcaaaaca ctgttttgag gttgcagata gaactgacga
    18961 agtcagctca aaacactgtt ttaaggttgt ggatggaact gacgaagtca gctcaaaaca
    19021 ctgttttgag gttgtggata gaactgacga agtcagtaac catatacggc aaggcgacgt
    19081 tgacgtggtt tgaagagatt ttcgaagagt ataagtctct acataataaa acgcatatta
    19141 ccaaggttgt gtaacttgat actatgcgtt tttctttttt attcaatgtg ggttgtttga
    19201 ttagcaaagg cgataatagc ttgcttcaac tcatctccac tgagagtgcg gaactctcca
    19261 atcttttgat tgatggattc taaaggcatg acattaacct taccaacgaa aatcttatcc
    19321 ataacttgat tatcaacgag ttcggttgat accaagagtt cttcgtagag tttttcacct
    19381 gggcggattc caacttcaac gattggaatt tcactttcag tgtggccact tagaagcacc
    19441 atcttcttgg ccaagtcata aatcttgact ggtttgccca tatcaaggat aaagacttcc
    19501 ccatctttgg cataagcacc agcatggata accagacggc tagcttctgg aatggtcata
    19561 aaataacggg tcatgcggaa gtctgtcacc gttacaggcc caccttcagc aatctgacgt
    19621 tcaaagactg gaatgacact accacggcta ccaagaacat tcccaaaacg aactgcacag
    19681 taggttgatt ggctacgttg gttaaagcca gtgacaatca actccgccac gcgcttggtt
    19741 gctcccataa catttggtgg attgactgcc ttatctgtcg aaatcataac catcttagac
    19801 actttagctt catcaacagc cttagcaaca ttgtaagttc cacggatatt gtttttgaag
    19861 gcttcttttg gattgcgctc catcatagga acatgcttgt gggctgccgc atgataaaca
    19921 atagcaggtt tgtactgctc aaagacttgc aacaaacgat catagtcttg aatgtccgca
    19981 atcacaggta cataatc
//
