LOCUS       NZ_FHRS02000009         5965 bp    DNA     linear   CON 12-DEC-2024
DEFINITION  Streptococcus pneumoniae strain GPSC18 substr. ST1797
            ERS455146SCcontig000009, whole genome shotgun sequence.
ACCESSION   NZ_FHRS02000009
VERSION     NZ_FHRS02000009.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2016) WTSI, Wellcome Trust Sanger Institute, CB10
            1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900055005.2-RS_2024_12_12
            Annotation Date                   :: 12/12/2024 02:43:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,241
            CDSs (total)                      :: 2,189
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 73 of 180
            Pseudo Genes (incomplete)         :: 93 of 180
            Pseudo Genes (internal stop)      :: 57 of 180
            Pseudo Genes (multiple problems)  :: 36 of 180
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:31:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 5965
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FHRS02000009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..5965
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..5965
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..5965
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[98:965](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      99..965
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            99..353
                     /gene="blpM"
                     /locus_tag="A1898_RS04960"
                     /old_locus_tag="SAMEA2521606_01006"
     CDS             99..353
                     /codon_start=1
                     /gene="blpM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /locus_tag="A1898_RS04960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01006"
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="WP_000379879.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 2.4e-20,
                     bitscore: 65.1, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGLG
                     TAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            369..572
                     /gene="blpN"
                     /locus_tag="A1898_RS04965"
                     /old_locus_tag="SAMEA2521606_01007"
     CDS             369..572
                     /codon_start=1
                     /gene="blpN"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /locus_tag="A1898_RS04965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01007"
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="WP_001099492.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 1.3e-13,
                     bitscore: 43.5, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGPW
                     GAAIGGIGGAVVCGYLAYTATS"
     gene            816..965
                     /gene="blpO"
                     /locus_tag="A1898_RS04970"
                     /old_locus_tag="SAMEA2521606_01008"
     CDS             816..965
                     /codon_start=1
                     /gene="blpO"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /locus_tag="A1898_RS04970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01008"
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="WP_001813641.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 2.4e-11,
                     bitscore: 36.3, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTKMMSQFAVMDNEMLACVEGGDIDWGRKISCTAGVAYGAIDGC
                     ATTV"
     gene            1069..1188
                     /locus_tag="A1898_RS04975"
     CDS             1069..1188
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /locus_tag="A1898_RS04975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="WP_000346296.1"
                     /transl_table=11
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     misc_feature    complement(1324..1337)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.08"
                     /tool="antismash"
     gene            1974..2357
                     /locus_tag="A1898_RS04980"
                     /old_locus_tag="SAMEA2521606_01009"
     CDS             1974..2357
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /locus_tag="A1898_RS04980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01009"
                     /product="hypothetical protein"
                     /protein_id="WP_000877378.1"
                     /transl_table=11
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVFYSGLFEAGVF
                     LLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHIFI
                     VFNALMYLLSSSQVSLKKNKLSS"
     gene            2409..3098
                     /locus_tag="A1898_RS04985"
                     /old_locus_tag="SAMEA2521606_01010"
     CDS             2409..3098
                     /EC_number="3.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /locus_tag="A1898_RS04985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01010"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_000760541.1"
                     /sec_met_domain="Abi (E-value: 1.3e-11, bitscore: 37.2,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKYQLLFKTSAILSYLFFVFGLSQLTLIVQNYWQFSSQIGNLFW
                     IQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKHVQ
                     STAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDLLLP
                     SILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFPFLLT
                     FLHRVLG"
     gene            3140..3373
                     /gene="blpZ"
                     /locus_tag="A1898_RS04990"
                     /old_locus_tag="SAMEA2521606_01011"
     CDS             3140..3373
                     /codon_start=1
                     /gene="blpZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /locus_tag="A1898_RS04990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01011"
                     /product="immunity protein BlpZ"
                     /protein_id="WP_000276498.1"
                     /transl_table=11
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFNF
                     LNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            3524..4135
                     /locus_tag="A1898_RS04995"
                     /old_locus_tag="SAMEA2521606_01012"
     CDS             3524..4135
                     /EC_number="3.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /locus_tag="A1898_RS04995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01012"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_000394045.1"
                     /sec_met_domain="Abi (E-value: 2.9e-21, bitscore: 68.2,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSLL
                     VTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHLAE
                     VSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSNVPS
                     FIFYLLGGLLLGFAYKKSQNLWVSTLVHMLYNSWPLLYYL"
     gene            4296..5090
                     /gene="ccrZ"
                     /locus_tag="A1898_RS05000"
                     /old_locus_tag="SAMEA2521606_01013"
     CDS             4296..5090
                     /EC_number="2.7.1.15"
                     /EC_number="2.7.1.229"
                     /GO_function="GO:0004747 - ribokinase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /GO_function="GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /GO_process="GO:0051726 - regulation of cell cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ccrZ"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /locus_tag="A1898_RS05000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01013"
                     /product="cell cycle regulator CcrZ"
                     /protein_id="WP_000363002.1"
                     /sec_met_domain="APH (E-value: 1.6e-19, bitscore: 63.1,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGLA
                     REQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQLS
                     RLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDVRHS
                     NWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTVLSKL
                     YWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            5087..5722
                     /gene="trmB"
                     /locus_tag="A1898_RS05005"
                     /old_locus_tag="SAMEA2521606_01014"
     CDS             5087..5722
                     /EC_number="2.1.1.33"
                     /GO_function="GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /gene="trmB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /locus_tag="A1898_RS05005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01014"
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="WP_001266078.1"
                     /transl_table=11
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVEV
                     GSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEAGVSNIKLLWVDGSDLTDYF
                     EDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFEYSL
                     VSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
ORIGIN
        1 attcaagatt tggatgaaaa aaattaatag atactgttat actaaacttg tcaagtttgt
       61 aacaagacaa atattaaaaa taaaaaagag tatttgtcat ggatacaaaa attatggaac
      121 aatttcatga aatggatata actatgttat ctagtattga aggaggcaag aataattggc
      181 aaactaatgt cttagaaggt ggtggtgctg cttttggtgg ctgggggcta gggacagcta
      241 tttgtgctgc gagtggtgtt ggagcaccat ttatgggagc atgtggatac ataggagcta
      301 aatttggtgt ggatctttgg gcaggagtaa caggggcaac gggtggattt taataaaagg
      361 agacttgtat gaatacttat tgtaatataa atgaaacaat gctgagtgag gtttatggag
      421 gtaattccgg aggagcagct gtagttgctg ctttaggttg tgcagcgggt ggagtgaaat
      481 atgggagact tctaggacca tggggcgctg caataggagg aattggagga gcagtggttt
      541 gtggatattt agcctatacc gctacatcat aaaaatgaaa gctaaatttt atgaaggcta
      601 tattctttat tattctcttc gcttttcaaa cctatcttat ttatctatcc ataagtatct
      661 ccgataaaaa acaaaagact attgaattaa catcattgaa ttgttttgtc atattatttc
      721 taatctatga taaattaatt tttctcttta ttgcctatgt ttttttgata atttttatat
      781 taaacttgtt ccgtaactga aaagtgatat actatatgga tacaaaaatg atgtcacaat
      841 ttgcagttat ggataatgaa atgcttgctt gcgttgaagg tggagatatt gattggggaa
      901 gaaaaattag ttgtacagca ggggttgcat atggcgcaat tgatgggtgt gcaacaacgg
      961 tttgacattt ctattgggac catttgctat aggaataggt gtaactggtg ctgcaggtgg
     1021 agctatactt ggaggtgtag cctatgcagc aatatgttgg tggtaattat ggattttaaa
     1081 agttttatta ttggtttagt agttggtata tttggtcctt atatggatga cttaattaga
     1141 aaaatatttt caaaatcttc caaaaaggat acggatagta ctctctaaaa aataaatttc
     1201 tcccctgtat ttgctagctt aatttacagg ggagtttctt taatattgtt tcgcctgtca
     1261 aaatgatatg tgatagcagg atgaaaagtc tacaaacaag ttggtatcaa tacaatataa
     1321 agaggaaagt atgtttttaa tgcttacttt ttaaattttt taacaattca agatgtttcg
     1381 atgacaattc aagatttgga tgaaaaattt taaaaaacaa tgatatacta aacttgtcaa
     1441 agttgcaacg ggtgctgcta cattatgtta aaaatcgctc ttgattgata agctggattt
     1501 gttaggaaag gagtaaataa ttatggataa gaaaaagata gtttcgacta taatatgtat
     1561 agtatttctt gtagtgtcag tagataattt ttttagagac ttaaccccgt tactgttcat
     1621 tttaaatatt attggcctgt cttgcttttc agtgctaaca tacattaaca taaaagagat
     1681 actcttaaat ataagtaaat gatgatttgg ttagtcctag aaccaatcaa cttatgttta
     1741 tcggcttggc agattttatg tttgtcattt gtttgtatag aggtatttca gaaacagaat
     1801 tctatcaaca actaatcgct tatataggtg tattctctgc ttgtttatca cgcttctgct
     1861 catgtggggc ttgaagtatt ataaaaagta gctttctatc cttttctgac aggatatttg
     1921 agtagaaggc tctattaaat tgttagtgaa ataaatgaat ggaggtattt aatatgaaat
     1981 acagattatt ttttgttatt ttcttgagta gtgtgttgga tattctttta gggacatttt
     2041 tacaaatctc tatcgtatct attgggtggc ttgtttttta cagtggtttg tttgaagcag
     2101 gagttttcct tcttgctaat aaaggggtgg cggtaaaaat caaggaagta gatattcgaa
     2161 atcgctttaa atttattttt ggaaaaacct tatggtttca aattcttttg ctcatctttt
     2221 tgataatcaa actttatctt ggtttggatg cgaggttgat tttattctat ggacatattt
     2281 tcattgtctt taatgcctta atgtatctat tatctagtag tcaggttagc cttaaaaaaa
     2341 acaaactgtc ttcttaatct tacagcagta gagcctgaca aggtgaggtc atagtaggga
     2401 ggatttctat gaaaaagtat cagcttctat tcaaaacaag tgcaatttta tcctacctat
     2461 ttttcgtatt tggtctttct cagctgacgc ttatcgtcca aaactattgg caattttctt
     2521 ctcagatagg caatttattc tggattcaaa atatcttgag tttacttttt attggagtca
     2581 tgattgtggt tcttgttaag acaggccatg gttatctctt ccgcattcca agaaaaaaat
     2641 ggctttggta ttcgattttg acagtattag tggtagtgtt ccagatctct tttaacgttc
     2701 agacagctaa acatgttcag tcaactgcgg aaggttgggc tgtattgatt ggttatagtg
     2761 ggactaactt tgcagagcta ggtatttata tagccctgtt ctttctggtt ccactgatgg
     2821 aagaattgat ttatagagga ctactgcaac atgctttctt taagcattcg cgatttggtc
     2881 ttgatttgct tcttccttct attttatttg ctctccctca tttttcaagc ctgcctagtc
     2941 tgttagatat cttcgtcttt gcaacagttg gaatcatctt tgctggtttg acccgctata
     3001 ccaagagcat ttatccatcc tatgcggtgc atgtgatcaa taatattgta gcgaccttcc
     3061 cgtttttgct cacttttcta catagggtct tggggtaaaa aaacaaaagg cttgcttttc
     3121 agccatagag gaggtcatca tgtataaaca cttatttttc ctagattcca aaactttaga
     3181 tcggttgaca ccctatattc tagtcttggc ttctgacacc attgctttta atgtttttgt
     3241 gctaaccttt gtatctgcgg tggtttttaa tttcctaaat tccatgctag ctttaatggc
     3301 tatattcata ggggctggct atgtggtcgg attttggtta ctaatactca atgaaaatca
     3361 aagagcaaac taggaaacta gctgcaggct gtacttgagt acggcaaggc gacgttgacg
     3421 tggtttgaat ttgattttcg aagagtataa aatggtttgt tttggaaagg ctagaactaa
     3481 agaatgacgt gtaggaagtc tgtttgttga ggaggataat tttatggagt tttttgataa
     3541 atttcatgcc ttgtgttttg gatttttagt actaataatt gtcattacag ttccttatac
     3601 gattaaccat gggggttttt ttcaaaatga atctgcattg attcttgtaa gtcttcttgt
     3661 aacctcgctg agtgttgctt atgctagaaa gtttgaaatg atttcttttg ggatgttaag
     3721 caagaaacaa cttttgcttt tcattgcaat ctttcttcta agtgtacttg agacgctggt
     3781 ttatattcat ttcttcgctg tttcttctgg ctcaggggtc caacacttgg cggaagtcag
     3841 cagaggaatt tccctgtctt tgattttaac tacctcagtt tttggtccca tccaggagga
     3901 actcattttc agaggacttc ttcaaggtgc ggtttttgac aattcttggt tagggcttgt
     3961 gctaacttcc tctctctttt ctttcatgca tggaccttct aatgtccctt cgtttatttt
     4021 ttatctactt gggggtttgt tgctgggctt tgcttataaa aagagtcaaa acctatgggt
     4081 ttctactcta gtccatatgc tttacaacag ttggccactc ttatattatt tataaaaatt
     4141 atgaaaaggt aagcagaaga cggtgcttac ctttttcaag ctataatgat acccaagcca
     4201 atccagaggc ttttctttga gaatcgggtt agagtggttg acgaataggc caaaaactag
     4261 tagaatagta aggaaacttt atacggagga aagaaatgga tttgggtgat aatgagctaa
     4321 cactgactcc catacctggg aaaagtggca aggcttatat gggtagctat cctgatggga
     4381 agcgcatctt tgtaaaaatg aacacctctc caatcctacc tggtctagct agagaacaaa
     4441 ttgctccaca attattatgg agtcgtcgtt tggcagatgg gcgtgatatg tgtgctcaag
     4501 aatggttgac aggcaagata ttgaccccct atgatatgaa tcgtaagcaa atcgtcaata
     4561 ttttaacccg tcttcatcgc tcacgtccgt tgatgacaca attgagtcgt ttgggctatg
     4621 ccatggaaac acctgtagat ttactacagt cttggcagga aacggctcca gatgctttgc
     4681 gtaaaaatca ttttatcagt gaagtgatgg ctgatttacg tcagactatt ccaggattta
     4741 gagaggacca tgcgaccatt gtccatggag atgtacgaca tagtaattgg attgagacag
     4801 atagtggctt gatttattta gtagattggg attcggttcg cttgaccgat cgcatgtttg
     4861 atgtggccca tatgctctgc cattatattt cagaacatca gtggaaggaa tggttgacct
     4921 actacggtta caagtacaat caaacggtat taagtaaatt gtattggtat ggtcaattgt
     4981 cttatttgag tcagatttcc aagtattata tgaaccaaga tttagaaaat gtcaatcggg
     5041 agattcatgg tttgcgtcat ttccgagaca agtatggaaa gagaagatga gagttagaaa
     5101 tcgtaaaggg gcaacagaat tactagaggc aaatccccag tatgtggtcc tcaatccctt
     5161 ggaagccaag gcaaaatggc gggacttgtt tggcaatgat aatcccattc atgtggaagt
     5221 tggaagtgga aagggtgcct ttgtttcagg tatggccaag caaaaccctg acatcaacta
     5281 tatcgggatt gatattcaaa agtctgtttt gagctacgct ttggacaagg tgcttgaagc
     5341 tggagtgtct aacatcaagc tcttgtgggt agatggttct gacttgactg actattttga
     5401 agacggtgag attgatcgct tgtatctgaa cttttcagat ccttggccta aaaaacgcca
     5461 tgaaaagcgt cgtttgactt acaagacctt cttggatacc ttcaagcgta tcttgcctga
     5521 aaatggagaa attcatttca agacggataa ccgtggcttg tttgagtata gcctagtgag
     5581 cttttctcaa tatggcatga aactcaatgg tgtctggcta gatttgcatg ccagtgattt
     5641 tgaaggcaat gtcatgacag aatacgagca aaaattctcc aacaaggggc aagttatcta
     5701 ccgagttgag gcagaatttt aagaaatagc ctgaaatcag gctgtataag tgcttttgct
     5761 ttacataagt tagcaaatgt gctatactaa gggtaagaat atgagaaaga gaggcgggga
     5821 aatatcttcg cctcttgctt atgaggaggt ggacgcaatc gcaacaatcg tagaattagt
     5881 cagagaagtt gtagaacctg tcatagaagc tcctttcgaa ctcgtggata tcgagtatgg
     5941 aaagattggc agtgacatga ttctc
//
