LOCUS       NZ_FHRS02000008        10297 bp    DNA     linear   CON 12-DEC-2024
DEFINITION  Streptococcus pneumoniae strain GPSC18 substr. ST1797
            ERS455146SCcontig000008, whole genome shotgun sequence.
ACCESSION   NZ_FHRS02000008
VERSION     NZ_FHRS02000008.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2016) WTSI, Wellcome Trust Sanger Institute, CB10
            1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900055005.2-RS_2024_12_12
            Annotation Date                   :: 12/12/2024 02:43:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,241
            CDSs (total)                      :: 2,189
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 73 of 180
            Pseudo Genes (incomplete)         :: 93 of 180
            Pseudo Genes (internal stop)      :: 57 of 180
            Pseudo Genes (multiple problems)  :: 36 of 180
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:31:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 82396
            Orig. end                         :: 92693
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FHRS02000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10297
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10297
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10297
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5297](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5297)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(68..946)
                     /gene="rsgA"
                     /locus_tag="A1898_RS04900"
                     /old_locus_tag="SAMEA2521606_00994"
     CDS             complement(68..946)
                     /GO_function="GO:0003924 - GTPase activity [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_function="GO:0043022 - ribosome binding [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rsgA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /locus_tag="A1898_RS04900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_00994"
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="WP_001161982.1"
                     /transl_table=11
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVDF
                     SAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHKGI
                     HPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGVGKS
                     TLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVSRAED
                     LNQDFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEIENRRE
                     TYKKVSKKIPK"
     gene            complement(948..1820)
                     /gene="rsmA"
                     /locus_tag="A1898_RS04905"
                     /old_locus_tag="SAMEA2521606_00995"
     CDS             complement(948..1820)
                     /EC_number="2.1.1.182"
                     /GO_function="GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="rsmA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /locus_tag="A1898_RS04905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_00995"
                     /product="16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="WP_001216869.1"
                     /transl_table=11
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEID
                     DQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDILKV
                     DLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRISAQ
                     PNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVEDENFF
                     FKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLAEFAGL
                     ADALKGQGL"
     gene            complement(1951..2247)
                     /locus_tag="A1898_RS04910"
                     /old_locus_tag="SAMEA2521606_00996"
     CDS             complement(1951..2247)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /locus_tag="A1898_RS04910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_00996"
                     /product="hypothetical protein"
                     /protein_id="WP_029744667.1"
                     /transl_table=11
                     /translation="MRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYLLDFAS
                     IEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC"
     gene            complement(2251..2892)
                     /locus_tag="A1898_RS04915"
                     /old_locus_tag="SAMEA2521606_00997"
     CDS             complement(2251..2892)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /locus_tag="A1898_RS04915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_00997"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000565531.1"
                     /transl_table=11
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLLN
                     ILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFVGQ
                     KISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILADEPT
                     AALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(2894..4924)
                     /locus_tag="A1898_RS04920"
                     /old_locus_tag="SAMEA2521606_00998"
     CDS             complement(2894..4924)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /locus_tag="A1898_RS04920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_00998"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_000825275.1"
                     /transl_table=11
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMTNQ
                     PVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGELPEGLTISSKES
                     AETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLLSLA
                     IFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGIALRPVLEDVRQLICSVLVSSLLGLG
                     ILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGKLPLKR
                     MMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGWSSAFAD
                     EEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTPSGNVIYV
                     SPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYLQNFSSESV
                     EVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQATGTRPVAGML
                     WGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRVEFYSLLIGTI
                     LTLSTAILLFDSMNLLYFEQFRRDLMIKRLAGMTIYELHGKYLLAQGGVLLLGLVLSSI
                     LTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(5001..5297)
                     /locus_tag="A1898_RS04925"
                     /old_locus_tag="SAMEA2521606_00999"
     CDS             complement(5001..5297)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /locus_tag="A1898_RS04925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_00999"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000815394.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 2.6e-21,
                     bitscore: 68.6, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQTVKKLALVASIAATLGGSVAVASAAVQYPEGGVWTYGSGNGG
                     AYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     misc_feature    complement(5447..5460)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 22.05"
                     /tool="antismash"
     misc_feature    complement(5537..5546)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     gene            complement(5617..6516)
                     /locus_tag="A1898_RS04930"
                     /old_locus_tag="SAMEA2521606_01000"
     CDS             complement(5617..6516)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /locus_tag="A1898_RS04930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01000"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000904832.1"
                     /transl_table=11
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPSL
                     PKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEIYK
                     TYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLLIRL
                     FFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVGVTDC
                     FNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAQLIGDE
                     FLVKKLTEEWQEDVKKYL"
     gene            complement(6588..7148)
                     /gene="rnmV"
                     /locus_tag="A1898_RS04935"
                     /old_locus_tag="SAMEA2521606_01001"
     CDS             complement(6588..7148)
                     /EC_number="3.1.26.8"
                     /GO_function="GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="rnmV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /locus_tag="A1898_RS04935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01001"
                     /product="ribonuclease M5"
                     /protein_id="WP_000664448.1"
                     /transl_table=11
                     /translation="MKERISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERIQ
                     RLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEHAS
                     YEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSNGKQ
                     LLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(7148..7921)
                     /locus_tag="A1898_RS04940"
                     /old_locus_tag="SAMEA2521606_01002"
     CDS             complement(7148..7921)
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /locus_tag="A1898_RS04940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01002"
                     /product="TatD family hydrolase"
                     /protein_id="WP_000575600.1"
                     /transl_table=11
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVIQMNIVGFDKPTIER
                     ALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTAPK
                     EVQEQVFRRQIRLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLEWAE
                     KFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENKTAYT
                     RYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(8226..>8945)
                     /locus_tag="A1898_RS04945"
                     /old_locus_tag="SAMEA2521606_01003"
                     /pseudo=""
     CDS             complement(8226..>8945)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_367670414.1"
                     /locus_tag="A1898_RS04945"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01003"
                     /product="DUF1542 domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="VEVGGDGTATITFQDGSVVTILGKDTVQQSAKGESVTQEATPEYK
                     PETTPGGDKGSNTGSSDANANAGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKNAI
                     EKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGVQAI
                     AMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALASLGLV
                     VATSGFALLGRKTRRRK"
     gene            complement(9154..9288)
                     /gene="rpmH"
                     /locus_tag="A1898_RS04950"
                     /old_locus_tag="SAMEA2521606_01004"
     CDS             complement(9154..9288)
                     /GO_component="GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA]"
                     /GO_component="GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA]"
                     /GO_function="GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rpmH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /locus_tag="A1898_RS04950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_01004"
                     /product="50S ribosomal protein L34"
                     /protein_id="WP_000831905.1"
                     /transl_table=11
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA"
     misc_feature    complement(9710..9725)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 20.67"
                     /tool="antismash"
     misc_feature    complement(9787..9797)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
ORIGIN
        1 ttcaaagttg gcataatctg ctgccagaat tgacggagca atcttgtatt gagacatagg
       61 tttctcctta ttttggaatt tttttgctga cttttttata ggtttctcta cgattttcaa
      121 tttcactaag gaattgcagg taattgtcaa aacggaaggt tgcaataaca ccctcttcaa
      181 cagctggttt gacggcacaa gacggctcat gggtatgggt acaagtacgg aacttacaat
      241 ctcggctaac agtagcaatc tctgggaaat cctgattgag gtcttcagcc cttgatactt
      301 catagtccaa ggatgaaaat cctggtgtat ctgcgatttt acccccgttg agattgtaaa
      361 aactaacagc tcgagtggta tggcgaccgc gacctagact gtctgaaatt tctcccgttt
      421 caagattgag gtctggtgcg attttattga gaagagttga cttcccaaca cctgtctgcc
      481 ccataaagac cgtaaccttg cctgttaaca aagacaggag ttcctcttta ctggtcacaa
      541 agtcatagcc gatgtcacca taggtctgct ggtaaaaatc cagttctccc ctatcttcca
      601 acaaatccat tttggaaata tagacaatgg gatggatgcc cttgtgctcc aaaagaacca
      661 agaaacgatc cagcaaattg ctgttaaaat caggttcctt gacggacatg attactacag
      721 cttgatcgat attgacaata ggcggacgaa ccagactgtt tttccgttcg tgaattttga
      781 ggatatagcc ttctgaattt tcctcggcag agaaatctac ccagtcccca acataagggg
      841 tatggccttt tttacggaaa ttcccacgcg cgcgtgtttg ataaacctgg ccatcactct
      901 ccacatagta gaaacctgcc aaggctttaa tgatttgtcc ctgcatctta gagtccttgc
      961 cctttaagtg cgtctgctag accggcaaat tctgccaagc tgagagcttc cccacgcaca
     1021 cttggtgaca agcctgcctg gtccaaagcc ttggtcagct tgtccttgac ctcttcagtc
     1081 ttaccaaagt aacctgtcaa gttattccac aaggtcttgc ggcgatgggt aaaactagcc
     1141 ttggaaacct taaagaaaaa gttctcatct tctactgcta cggctggctc tggacgacgc
     1201 accattttca agatggctga atccacattt ggcgctggca caaagaccgt acgaggcacg
     1261 ataaaggcaa ccttggctgt catgtaatac tgcacggcga tagacaagct accgtaagcc
     1321 ttggtgttag gctgggctga aatgcggtcc gctacttctt tctgcatcat gaccacaaac
     1381 tcactaaaag gaatgccact ctcaatcaag tgcatgagaa taggcgtcgt gatgtagtaa
     1441 ggcaaattag ccactacctt gattggcagg ttaggatttt taaaattttg gatatgttgc
     1501 gccaaatcaa ccttgagaat atcttcgtta actacggtca cattatcaaa atcacgcagg
     1561 gtatctgcca aaattggcac caaacggtgg tcaatctcaa aagccatgac ttgggctgca
     1621 cgctcagcca aaaattctgt caaggcacca atacctggcc cgatttcgat gacattgacc
     1681 tgatcatcaa tttcagccgt atccacaatt ttttgaagga tattggtatc cgtcaggaaa
     1741 ttttgcccga aggacttttt aaaggtaaaa ccgtgacgct ccagcactgc cttggtcacg
     1801 ctataatctg caattctcat ctattctctt ttctattatc tgttactact attgtaacac
     1861 attccccttg tattttggga atgaaaatca taccaaaaaa ctatctttcc tcatgaagaa
     1921 agatagtctt ggaaatcttt aaaagtggcc ttagcagttc tctcgaaaca tgcgttcaaa
     1981 aaaatcagct tcttgataac ttcgattttc gggtagggtc aaatccacat cctcctccac
     2041 atcatccact gtcagttcaa ccgtctgagg taaatttttc aaacgataat gcttttgcct
     2101 ttgcccttca atactagcaa aatcaagcaa atagccatgc actcggattt gaaccaaaag
     2161 gagatgattg gtcatatttt ccatgatgag gatatcttct aaaccacctc tggcactagg
     2221 ataatcagat accctgcgaa tacggatttt tcacacatga gcaagtttcc tcatatcaat
     2281 gatttcatca gccttattcc agactagggg attatgggtc gcaatgatga taattcgatt
     2341 ttcatctttc aaatccacca agagattcat aacctcctct gaattttcag gatcaagagc
     2401 tgctgttggt tcatctgcca aaatcaaggg tggatttttc aaaatagtct tagcaagggc
     2461 aactcgttgg gcctctcccc cagataaagt atagattttt tgttctaaat ccaaataccc
     2521 tagattaact ttttctaaag cccccacttg cctttccaaa cgttctactt ttgagatttt
     2581 ctgaccaaca aaacccaaat ccaaattttc tttgattgat tggttttcta agaggccgaa
     2641 attttgaaag agatagccca tctggtctcg aaaatactca cgagtgggaa tggtttttaa
     2701 atctttcccc tgatagagaa cccttccacc atctatcttt tctagctttc ccaagatatt
     2761 cagcagcgtc gtctttccac ttccactctt tccgattaag gcataaacct tgcccttctc
     2821 cagcttgaga ttcaaaccag agaaaatcgc gcggtcatta aatttctttt ccaatccttg
     2881 aatatcaatc atcttatttt cctttcaata ctgccatgct accagcttct tcttttttgt
     2941 cctgccttac taaaatcaag agggcgttaa gcgtaaacaa agctacaact agagcgctaa
     3001 tcaaaccatc tcttgtcaaa atactagata ggactaggcc aagcaagaga actcctcctt
     3061 gcgccagtaa atacttgcca tgaagctcat agattgtcat accagcaaga cgtttaatca
     3121 taagatcccg tctgaactgc tcaaaataga gaagattcat ggaatcaaat aacaagatag
     3181 ccgtagacag ggtcaaaatc gtcccaataa ggagagagta aaactccacc cgtttgtcat
     3241 ttagtacctt ggcaaaaaat agctggcttt ttaccaagta agaaaccttg tgatacagac
     3301 cttgctcttt tagagctgtg atgctgtctc catatcgatc tagtttcaag gcactattag
     3361 ccgtagttcc ccacaacatc cctgcaacag gtcttgttcc agtcgcttgc ggcgttagaa
     3421 ctacaataat cggatctttt aggtactggc gtgttgtctt gtacccatca ttatagagga
     3481 aacgttcctg tcctgtttct gtaaaagcta gccttacctg tgggaggtag tgtttctgac
     3541 tcgtcacttc tacactttca gatgaaaagt tttgcaggta atctgtaaac aatccttggt
     3601 agtagacaga ctgctctcgc aagctctcag gcaagatcag cccaaactct ccctcagaca
     3661 agttttgcat cttgtccata aactctgaag aaacggtaat cttttcttct atcagatagc
     3721 gtggtgagac atagataaca ttccctgacg gtgtgtagtc actgagacga ttgccatcta
     3781 ggtccacttc tgctccatct gagaaattgt caacattgct cataatataa aaagagtctg
     3841 tattggctaa ccgttcttca gtaaatgtct gccactcacg attatcctta cgcgttcctt
     3901 cttcatcggc aaatgcacta gaccaaccaa aggatagacg gtaacggtct gaggactggc
     3961 tccatttatt gctagctctc tccatttcct gcatttcacg gtagtggggt aggagagctg
     4021 tcgcactcga tccgaccacc aatacagcta agagttgccc caccatcatc aatgtcatca
     4081 tacgtttgag agggagtttc cctttcaata gatccaccag actattttcc tgtaaaccaa
     4141 gtagatagac gacacttagt aaggtagaaa tccctgccaa ggtcaatcca taaagtagaa
     4201 gagcaatgat gaccagttgc accgttgcca taaacaaggc accttgatac cagagaatcc
     4261 ccaatcccaa aagactggat accagcactg agcagataag ctgtctcaca tcttctaaca
     4321 ctggtctgag agcaattcca aacaagctct caccagctat taagcgaatc cctgcctgac
     4381 gaagggattt gatccgataa atcagggtca gactcataaa ggtcagcaga aaaatagcta
     4441 aactcagtag caccataggg gtggccgcca atagtaagac tatcgaaaat ggatcttctc
     4501 cattcgaaac aaagccttga taaccaagct ctttcaaggt ggtctgtaag ctcactccat
     4561 ccaaacttcc tgacacaatc aagtaagacc ctaataaatc actcgtttct gcactctcct
     4621 tggaggaaat ggtcagacct tctggaagct ctccctcacc ataagtggca taggtaaagc
     4681 gtgtttctcc agcttcattt ggctcaacga ttcgacgggc gatgagacta ttatgctcta
     4741 ccgccaagtg actcaaagcc tctgtcacct cttcgcgact agctactggt tgatttgtca
     4801 tcattcctgt cacatcaaca gaaggatagt taccaaataa aatctcttct cgatggtcaa
     4861 cggaagttat caccatataa agcgatacca ataccattga aatcaaaata aataaacgtt
     4921 tcatcatttc ctccaaaaag ttacttggtc acttagtgag aaggtgacca agtaaagcta
     4981 aacaataggg ctataccatt ctaaacatta tagtagaatg caactttctc tccccaagaa
     5041 gtacgaatcc atgcacgaga agtccctcca gcaccagcat atcccttgtc agatgaacca
     5101 gtttttctgc taacaactgt tgaactatgg tattttgaag ggtgatagta gtttgagtaa
     5161 gcaccaccgt tacctgaacc ataagtccaa acaccacctt ctgggtattg aacggctgca
     5221 gaggcaactg ctacacttcc acctaatgtt gctgcaatgc tcgctacaag agctaatttt
     5281 ttaactgttt gtttcatatt gatactcctc tatttttgat tacaagattg ttgagttact
     5341 tgctcctctt tccattatca acgttgatac tattatatta ccaaatgata ttctaaaatt
     5401 atttgttttt ctctaaattg tcaaaatagc accctgaaat gaacttttat agtcattata
     5461 ctccatcttt atcacactat caaggacatc aatgtcctta aaatctaact atttcatatt
     5521 atttttagct tatatctaaa aaaacagttc taaagacgaa ctttagaact gttttgatac
     5581 gaggacaagg acatttttgt cactgattca ctatgtttat aaatattttt tgacatcctc
     5641 ttgccattcc tctgtcaatt tctttactag aaattcatct ccaattagct gcgcaaatgt
     5701 tttagaagac tgataaaatg attcagctaa actaaaatct ctttcttctc ttaatgcttg
     5761 cttccacaaa aacataaata caagaggttt cttttgataa tcttgagttt tatccataat
     5821 ttcttgtaaa cacgatagat aatcattaaa acaatcagtt actcctacct tttcaagaca
     5881 acacattcct gaaataagag tatctctaat tataaagtaa ttctctattt gagttacctt
     5941 cttctgttcc aaaagaaaga gcattagctt ttcgatttga tttataaatt tggcaagatc
     6001 ttttctaatg agcatttgat aaaaaaacaa acgaatcaac aacatatcat ttattgaata
     6061 agcttctttt tctataatct gaggcatatg ttcctcaagt attaactctg catattcagg
     6121 ccttctagtt ctagaagtat ccaaacacgc ctgcaatact tcacatgcta attgttcttc
     6181 tttaggaaga ttatcaaagt atgttttata aatctcttcc aaacacgctt ccttcttatc
     6241 gtactcctct tctttaccat agattggttc acgtaaaatc tggtatttca attctaaata
     6301 agcagaagga agagctgaaa aatccggcat aaggctataa actggaactc ctagccggcg
     6361 agcaatatag gctaacttgg gcaaactcgg ttgcgaagct ccactttcaa ttcgcgacag
     6421 ttgacgaact gtcagttctt gctcatctcc acaaaaatct ggacgactaa ttcctttttc
     6481 tatccgaatc tctttaattt tttgaccaat taacatcaga cgcctcctct tctacctgct
     6541 tcgaaaaata ctgtgaaact taaaaaattg taacggctac atctttccta ctcataagat
     6601 ttcatagctt cttccacttc tgccaaagta accccaaaca actctaggcg tttgaggagt
     6661 tgcttgccgt tagaatagcc gattcggaga gtctctccga gatattctct acgcttacgg
     6721 ctgtctgccc ctgctagaaa accaaggcga atcaaatcgc tacgactaat gtcaaactga
     6781 ctctcatgtt caaattgttc tgtcacctga gctagagccg ttttcaggtc ttcatagctg
     6841 gcatgctcaa ttcccagaga acgccccttg gtcttggact tgggaacagc ttcatctcgc
     6901 ttgagaaagg catgctgaac tgttggaatg gccgtcataa tcatgcgccg aatccgctcc
     6961 ccattaaaat ctgggtctgt aaagacaatg actccatgac gttggtgtag acgctgaatc
     7021 cgctctatgt cctggtcatt gatggcagaa cctcgagtct cataggtctc cacatcgaaa
     7081 taacgcttga gattgaccgt atcatcacga ccttcaacca cgataacttg ggaaattctc
     7141 tctttcatta cttgctgtcc aatccaaaaa tgcgttctgc atttgcagtc gttgctaccg
     7201 ccagctcttc tgtcgtcata ccacgcaagt cagcgataaa gtcgaccaca tagcgagtat
     7261 aggctgtttt attttcacga ccacgcttgg gtacaggtgc taagtaaggc gcatctgttt
     7321 ctaccaacat cttgtccaaa ggtaactctt tagctgcttc ttggaggtca gttgccttct
     7381 tgaaggtcac cactcctgag aaggaaatgg tcataccaag atccacaaac ttctctgccc
     7441 actcaagcgt ccctgaaaat gaatgcatga taccaccacg aggaccaacg ccctcactct
     7501 tgataatctc ataggtatct tccagcgcat cacgggtatg gacaacaaaa ggcaaatcca
     7561 agtccttaga tagccgaatc tgacggcgaa aaacctgctc ctgcacctct ttgggcgctg
     7621 tcatccaatg gtaatctaag ccaatttcac ctaaagccac aaccttggga tgttttaact
     7681 tatccaacaa gtaagcctca acttcctctg tataagtacc agcttctgta ggatgccaac
     7741 caatagtcgc atagagctgc tcatactcat ctaccaactc caaggcacgc tcaatcgtcg
     7801 gtttatcaaa accaacaata ttcatctgta tcacacccat ctcagcagcc aaggcaattt
     7861 cttctgcctc acgacctgca aattcttcta cattcaagtg tgtatgtgta tcaaaaatca
     7921 tctcttctaa cctcgttttc tatcttctat tataccaaaa aagaggaggg gcacctaatt
     7981 tttcggtttc ccctcctctc ttcaatagag agctattctg ctatcttttc tatccgatat
     8041 tgcccatctc ctattccaca gttagagaca gaagagattg gctacattga tagaatattc
     8101 ctattcagct aaagtatgtg attatcttgg gttctcactt ttctcaccca tcactaggat
     8161 tgatgacttc tcaaaatccc ccttataatc taactttaag tagagaatag aattttctag
     8221 ctgttctatt ttctacgtct agtctttctt cctaacaaag caaaaccact tgttgccacc
     8281 actagaccaa gactagcaag tgctgcactt gatgctgaac ctgtgttagg taattgtttg
     8341 ccagcaggtg actggtaggt aacatcttgc attgttcctg cagttgcttg cggtgcactt
     8401 gttgtcttag gagcagcatt aggagccact ggtctcttag gaactgtaac catggcaatg
     8461 gcttgcactc caatcgtttc tgcttccttc acatcttcca tagttttcgc attttcaatc
     8521 tctttgagag ctgcttgttt ttctgcttcc actcttgcta aaagttctgc tttttcttta
     8581 tcagaaagcg gtgcgccttt gatttcatcc tgcttatcct tggctgcttt ttcaatggca
     8641 tttttagctg attctttttc agtagctaat tgcttagaag cttgtgattg agctgagttt
     8701 tgtgaacctg tgtgagctga tccacctgcc tggctaccac cgcctgcatt cgcattagca
     8761 tctgagcttc cagtattgct tcccttatct ccacctggtg tagtttctgg cttatactct
     8821 ggtgtagctt cttgagttac agattcacct ttcgcagatt gttgaactgt atcttttcct
     8881 agaatcgtca ctactgaacc atcttggaat gtgattgttg ctgtaccatc tccacctact
     8941 tcaactttat agtgaaataa aataataaca ggacaaatcg atcaggacag tcaaatcgat
     9001 ttctaacaat gttttagaaa cagaggtgta ctattctagt ttcaatctac tataacatcg
     9061 cttccgaatc catttgcatc tactgaactt ttttacaaaa ataaaaatca gtagactcga
     9121 gttcctactg atttttgata gaattcgttt ggattatgca gccaaaactt tgcgtccttt
     9181 acgacgacga gctgccaata cgcgacgacc atttttagtt gacatacggt tacggaatcc
     9241 gtgtttgcgc gcacgacgaa gtttacttgg ttgataagta cgtttcacga tgaatacctc
     9301 ctcatagatt ttgtattcgt ttagccggct ataaagtgat cagttactaa acatacttta
     9361 ctattctatc tgattctttc acttttgtca atagctctag gcaaatgttt ccagcttttt
     9421 cgaatgcaag ccctattgaa agttctataa tgaagttagc caccttacct tagtcaagaa
     9481 ttagatgttt cactatgttt gagtaagttg atgatttcat ttgataacag gtttgaaact
     9541 gttaggctag gtggccaagg ctaatcatag ccttggttta gctgaaaaaa caggttcaag
     9601 ggttcctgtt gtcaaatgaa atgtgattta aggtataaga aaacacctct gtgctatact
     9661 tgttgttcac cacaaacaca aggaaaggca cagagatgca agaacattat accccaaaag
     9721 ggaaacattt gacaatagat aaccgtcgct tgattgagcg gtggaagaat gaaaataagt
     9781 ccaatcgtga aattgcaggc ttgttaggaa aggcgcctca aacgattcat aatgaagtca
     9841 aaagaggtac aactttacaa caagtgagaa aagggctata caaaaaggtc tattctgccg
     9901 attacgcaca aactgtttac caattcaatc gaaaacggtc ggtgaaaaag ttaattttaa
     9961 caaaggaaat cagagagaag atcttacact atcataagca aaaattttcg cctgaaatga
    10021 tggttaacaa gaagcaagtg aaagttggta tttcaaccat ctactactgg tttcataatg
    10081 gtcatttagg attgacgaag gccgacatgc tttatcccag aaaaaggaaa ggtgtcaaga
    10141 agcaagctag tccgaacttt aagccggcag gtaaatctat cgaagaacgt cctgacgtta
    10201 ttaatcttcg cttggaaaat ggtcattatg aaattgatac cgtcctactg actaagataa
    10261 aaaattattg cctgttagtc ttaaccgacc ggcggag
//
