LOCUS       NZ_FHRS02000001        10300 bp    DNA     linear   CON 12-DEC-2024
DEFINITION  Streptococcus pneumoniae strain GPSC18 substr. ST1797
            ERS455146SCcontig000001, whole genome shotgun sequence.
ACCESSION   NZ_FHRS02000001
VERSION     NZ_FHRS02000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2016) WTSI, Wellcome Trust Sanger Institute, CB10
            1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900055005.2-RS_2024_12_12
            Annotation Date                   :: 12/12/2024 02:43:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,241
            CDSs (total)                      :: 2,189
            Genes (coding)                    :: 2,009
            CDSs (with protein)               :: 2,009
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 73 of 180
            Pseudo Genes (incomplete)         :: 93 of 180
            Pseudo Genes (internal stop)      :: 57 of 180
            Pseudo Genes (multiple problems)  :: 36 of 180
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:31:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 124494
            Orig. end                         :: 134794
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FHRS02000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10300
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10300
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10300
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5300](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5300)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    404..418
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: weak, score: 16.66"
                     /tool="antismash"
     gene            complement(810..2162)
                     /gene="murD"
                     /locus_tag="A1898_RS00680"
                     /old_locus_tag="SAMEA2521606_00143"
     CDS             complement(810..2162)
                     /EC_number="6.3.2.9"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA]"
                     /GO_process="GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /GO_process="GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /gene="murD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /locus_tag="A1898_RS00680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_00143"
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="WP_000863033.1"
                     /transl_table=11
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFED
                     NPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTEVE
                     LAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASDKDT
                     LVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAADFLVL
                     NFNQDLVKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPGSHNVE
                     NALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNILATQKA
                     LSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKAGVAYVEA
                     TDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE"
     gene            complement(2266..2907)
                     /locus_tag="A1898_RS00685"
                     /old_locus_tag="SAMEA2521606_00144"
     CDS             complement(2266..2907)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /locus_tag="A1898_RS00685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_00144"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000571283.1"
                     /transl_table=11
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLLN
                     MIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLVGK
                     KLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILADEPT
                     ASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(2909..4930)
                     /locus_tag="A1898_RS00690"
                     /old_locus_tag="SAMEA2521606_00145"
     CDS             complement(2909..4930)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /locus_tag="A1898_RS00690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_00145"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_000732436.1"
                     /transl_table=11
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKDT
                     QEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQES
                     RERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILISLA
                     IFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFLGGGV
                     VLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGRLPLKR
                     LMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGLGDRGKD
                     TENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANTLYVSPSY
                     LDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYGKSGEEASA
                     PLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDSFISLSSWSI
                     NAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETATLILGAIVGI
                     ASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFVFGASLFILSSR
                     DLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            complement(5001..5300)
                     /locus_tag="A1898_RS00695"
                     /old_locus_tag="SAMEA2521606_00146"
     CDS             complement(5001..5300)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /locus_tag="A1898_RS00695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_00146"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000850381.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 1.1e-26,
                     bitscore: 86.9, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTKKHRLLALALISNFTLLGAASAAVQYPDGGVWTYGEGSGGGW
                     AFSNYYHGKKYHYSSLVSRWNSHSDKGEASAGKTSYAWIWTKWGEQVAFYCDYD"
     misc_feature    5482..5498
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 24.88"
                     /tool="antismash"
     misc_feature    complement(5488..5501)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.31"
                     /tool="antismash"
     gene            complement(5664..5918)
                     /locus_tag="A1898_RS00700"
                     /old_locus_tag="SAMEA2521606_00147"
     CDS             complement(5664..5918)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /locus_tag="A1898_RS00700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_00147"
                     /product="DUF3165 family protein"
                     /protein_id="WP_000262800.1"
                     /transl_table=11
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVLS
                     FLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(5938..7779)
                     /gene="typA"
                     /locus_tag="A1898_RS00705"
                     /old_locus_tag="SAMEA2521606_00148"
     CDS             complement(5938..7779)
                     /GO_function="GO:0003924 - GTPase activity [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /GO_process="GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="typA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1190: GTP-binding protein LepA"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /locus_tag="A1898_RS00705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_00148"
                     /product="translational GTPase TypA"
                     /protein_id="WP_000164111.1"
                     /transl_table=11
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAERA
                     MDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVVDA
                     YEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQLDF
                     PVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLDYNDF
                     VGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAGDLIAV
                     SGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSRKVEERL
                     QAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEVIVKEIDG
                     VKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPARGLIGYST
                     EFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMSIEERGTIFV
                     NPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPRILTLEESLEF
                     LNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            7941..8666
                     /locus_tag="A1898_RS00710"
                     /old_locus_tag="SAMEA2521606_00149"
     CDS             7941..8666
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1154: Pseudouridine synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /locus_tag="A1898_RS00710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_00149"
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="WP_001234997.1"
                     /transl_table=11
                     /translation="MRLDNLLAQEKISRKAMKQALLKGDILVDGCPARSLAQNIDTGLQ
                     ELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLDRD
                     TTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVCKPA
                     KLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLAEGNY
                     RPLNQKELQIIKNYLEMSR"
     gene            complement(8706..8933)
                     /locus_tag="A1898_RS00715"
                     /old_locus_tag="SAMEA2521606_00150"
     CDS             complement(8706..8933)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /locus_tag="A1898_RS00715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_00150"
                     /product="hypothetical protein"
                     /protein_id="WP_000660078.1"
                     /transl_table=11
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKESQ
                     LDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(9022..9402)
                     /locus_tag="A1898_RS00720"
                     /old_locus_tag="SAMEA2521606_00151"
     CDS             complement(9022..9402)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1163: rhodanese domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /locus_tag="A1898_RS00720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_00151"
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="WP_000259256.1"
                     /transl_table=11
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQL
                     IDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLKKQ
                     GFSEIYILSYGLDSWKGKVKTS"
     gene            complement(9402..9671)
                     /locus_tag="A1898_RS00725"
                     /old_locus_tag="SAMEA2521606_00152"
     CDS             complement(9402..9671)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /locus_tag="A1898_RS00725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMEA2521606_00152"
                     /product="YqgQ family protein"
                     /protein_id="WP_000051787.1"
                     /transl_table=11
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIEL
                     MKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
ORIGIN
        1 atcgccaaga gctcaaaaat cgtattggca ccacctcgtg tcacaacaat atcagccaat
       61 tccatcaagg gttgatagag atcggtcaca tagtcaacac gaaaaagatt ttgcctcaac
      121 tcattcaggc tagaatctcc agttagattg ataatattgt agcgctctgt tagttctttc
      181 ttatggtctg tcaccaattg gttaaagaca cgagcacctg cagaaccgcc aacaaacaat
      241 acagtcggca atttatgatt aaagtggctt tgaatatcca ccaattcatc tggttctgga
      301 tttttttgat ctgaaacctt ggtcactgct cccacatgct caaccttaga caaactcgaa
      361 gcttgctcaa aggttgaata catcttagtc gcaaatttat aggcgatttt attggccaag
      421 cccatagaca ggtcagattc gtgaataaag acaggcactc ctgacacacg cgcagcgata
      481 acaggcggta ctgagacaaa gccccccttt gaaaaaaggg tctgtggacg aagtcgcaac
      541 atgataaaga gcgattggac gattccccag ccaactttga agacgtccag catattttgc
      601 caagagaaat agcgacgcaa tttcccagtc gcaatggagt ggaaagtgac atccaaacct
      661 gacttaagga tttcttggtg ttcgatacca cgcttgtccc cgatatagtg gacttcccaa
      721 ccatcttcga tgaacttggg cattaacaaa agattgaggg taacgtgtcc aaccgtcccc
      781 ccacctgtaa agacaatttt tttcatattt tattctttta actccgctac tgtgtcgata
      841 aagaggtcgc cacgtacttc aaagttagca tacatatccc agctggcatt ggcaggacta
      901 agaagaacca catctccttg agtcgcaagc tcataggcct tgcgggtcgc atctgcaata
      961 tctgtcgcct ccacataagc aacaccagcc ttgtctgctg cccgtttgac acgttctgca
     1021 gattgaccca ggatgaccat cttcttgagt ccagtaatat ctggcaccaa ttcgtcaaac
     1081 tcattgccac ggtccaaacc acctgcaatc aagacgacct tgctgttgtc aaatcctgac
     1141 aaggcttttt gagtagccaa gatattagtt gatttactgt cgttatagaa tttaacaccc
     1201 ttgatgtcat ccacaaactg gagacggtgt ttgacaccac cgaaggctga aagagtttcc
     1261 ttgatggttt gattgtccac accacgaagc ttggctacag caatagtcgc aagggcattt
     1321 tccacattgt ggctacctgg aacaccgatt tcattcgctg ccatgactac ttcaccacgg
     1381 aagtagagtt gaccatcttc cagataagct ccatcaacct tttcaagtgt tgaaaatggt
     1441 acaacagtgg cttctgtctt ggaagccaag tcttttacca agtcttgatt aaagttcaag
     1501 acaaggaaat cagctgctgt catcttgttc tggatattcc acttggctgc tacatattcc
     1561 tcaaataatc catgataatc gatatgagtt ggcatgaggt tggtaataac cgcaatctct
     1621 ggatggaatt cttgaacacc catgagttgg aaagaagaaa gttccataac aagcgtgtcc
     1681 ttatctgatg ctatttgagc aacctgactg gcaggatagc cgatattccc tgataaaaga
     1741 ccatgttgcc cagcagcagt caaaacttcc ccaatcatag tcgttgtggt tgtcttaccg
     1801 ttcgatcctg tgataccaat aatcggtgct tctgaaatca aataagccaa ttccacctca
     1861 gtcaagactg gaattccctt ggccaaagcc ttttcaatca tgggattgtt gtaggggata
     1921 cctggatttt tcaccataag ggcaaactct tcatccaaga gttccaaagg atggccacct
     1981 gtaatgacct tgatcccttc ttccagcaaa ctttgggcag ctggattgtc ctcgaaaggt
     2041 ttcccatcat ttactgtcac aatggcacct agcttgtcca acaaacgagc tgcagattca
     2101 ccagacttgg ccaaacctaa aacaaggact ttcttatttt taaattgatc tattactttc
     2161 atgtctcgaa ctccatttct actcctacta ttttaccatt tttatggaaa taaaaaagcc
     2221 acaaagtgtg tttgtgactc tttcttctaa ctgaatctta ccatatcatc tatgtgataa
     2281 atcggtaact cgaatgacct gatccacttg ctcccaaatc agaggattat gggtcgcaat
     2341 aataatggtc cgattcggat tttttaaaga ttctaggatg gaaagtaatt cctcagagtt
     2401 tttggggtct aaggaagcgg ttggttcatc tgcgaggatc aaaggtggat cctttaaaat
     2461 tatcttcgct agtgcaacac gttgtgcttc tcctcctgat aactcaaata taggttgctt
     2521 caaatccaaa taagagaggt ttacacggtt tagagcttgt ttcatcaaag agattttctc
     2581 tttttccttc aactttttac caactaaacc cagattgaga ttctctttga cggtttggct
     2641 ttcaattaag ccaaaatctt gaaataagta tcctaagtaa tctctaaaga aaacagaagg
     2701 cttaatgtcc ttaagagaag tgccatcata tatgatttgc cctttgtcat atggctctaa
     2761 tcgtccaatc atattcaaga gtgttgtctt accacagcca cttgtaccga ttaaggcata
     2821 aattttccca ccttcaaaat gaagattcgt atctgaaaat agctgacggc ttccaaattt
     2881 tttagatata ttctttagtt caatcatcct attttccttt cataattgtc atagaaacac
     2941 gagattcttt ctgcgcttga cggtaaagcg tcaaaactgc actagctaga aagaccaata
     3001 aagtgagcaa gccaatcacc aagtctcgac tgcttaaaat aaagagacta gcaccaaata
     3061 caaaactggc aaattggcta accatatact gagcatgtgt ttcaaaaaat cgtaaacctg
     3121 aaattcgttt aatcaagata tctcggcgga attgctcgaa atatagaaga ttgacagaat
     3181 aaaagagtaa caaggaactg gctattccaa caatagctcc taagattaaa gttgctgttt
     3241 cagtttgaac ttcattataa cgagttagat aaacacttct tccttcttta agataggata
     3301 cttgctcata aattccagct ttcttcaaga gttctagccc actctcatat cctttgataa
     3361 agagttgttt tccagcattg atagaccaac tagataagga tataaaacta tcacctgtag
     3421 aagtcggcgt gaataccact aaaatcggat cagtcaaata ctgagtggat acgggattct
     3481 caccgttatt ataaacaaac cgcttttctc ccattgaaag ataactaacg tgcgctttca
     3541 tctcataatc caaaggagca cttgcctcct caccagattt tccataataa ctcaatcttt
     3601 cttcaaaaac tttcttaagt tctgcttctc gagagcgcaa atgttctggg agcaagagga
     3661 taaactcacc tttttggaga tgggctaact tctgtttggt ctcagcatct accacgacct
     3721 tttccttgtc caaataactg ggactaacat agagcgtatt agcatctgga ctataggtat
     3781 ccagtgtctc tccctgttca ttttttcctt gtggattggc aaaatggagc agattatcct
     3841 ttacataaag agcttgttct tcttcgattg cttccttggc aaaggcatac cacttgctct
     3901 gattttctgt atcttttcct ctatcaccta agccaaaaga aatttgataa tagtctgctc
     3961 tatcctgcca tgcttgtttt gaaatttcaa gttctttcaa tcgttggtaa gacgtcaaac
     4021 ctgtcttaac agcgtagcct actgtaaaga cagcaaccaa ctgacacaat agggttaaaa
     4081 tcatcaggcg tttaaggggt aatctcccct taataacggg aactaatgct ttgtaactca
     4141 aactcattag ataaagaagc attagtaaaa ttgaaatcgc caataaaaac aacagataga
     4201 aactaatccc aaaaccatag gtggctaaca agataggata aaacaaacct tgactaaaaa
     4261 gaacgactcc cccacctagg aaggaaagga gggctgatag aaggagccat ttgatatcag
     4321 tagataaaga atgccccatg atggataaga gagtctgacc agaaaagagt tttatacctg
     4381 ctgctctcat ttccttaatc cgagtgataa tcactaaagc aaagaaagat aagccaaata
     4441 ttgctaaact aattaaaata aggggattta gtaatattcg aaaagcaaga aaatagggcg
     4501 gtatctttcg gtcagcactt gctttataac ccaaatctcc taatttatcg gcaagctttt
     4561 ctttcgtcaa ggagcctgac aaaaggagat aactatttag cggatcacta cgttcacgac
     4621 tttcttggct agcttcttgg aattcttttg gtaaagttcc ctgaccataa gttgcataag
     4681 taaagtgagt cgtcccatcc ttactcggct ctacaattct tctagctatt aaactctgtt
     4741 ctgagtttgc aaaattctcc aattcctgtt caaatacctc acgcgtcggt tcctgagtat
     4801 cttttttgac acgaagtaaa gaaacggaat catagcttgc atataaatat tgtggcgcac
     4861 gtaagacaat aatccaagca aggaagaagc tgagaaaaaa agttgataat aatatgaata
     4921 gtttcttcat agtagactcc ttgtaaacaa aattccccct gtaatttctt acaaggggaa
     4981 cgatttaaat caatgaacga ttagtcataa tcacagtaaa atgctacttg ttctccccat
     5041 ttagtccaaa tccatgcata ggaggttttt ccagcagaag cttctccttt atctgaatga
     5101 ctattccatc tacttacaag agaagaataa tgatattttt taccatgata gtaatttgaa
     5161 aaagcccaac cacctcctga accttctcca tacgtccata ctcctccatc tggatattgt
     5221 acagcagctg atgcagctcc caataatgta aaatttgaaa taagagctag agcaagtaat
     5281 ctatgttttt tcgttttcat tttatttttt ctttcaaaaa aagcacacct tgagcaacaa
     5341 tgcaacaaaa taaaccctcc tctctctttt gttgaaaccg ctttcttatg tgataaaaat
     5401 atcattttta ttatttgttg tcaaggaaaa aatcgaattt tttagatatt ttactacatt
     5461 gcctctatga ataatattat atagtagttt tatttcaaaa taatatgcaa ccagtactaa
     5521 ccaaatataa aatagatgcc attaaagaat tttattcaag tttttcccat tcatattatc
     5581 caaacaaaag agatggtgat aactcaaaaa taaattgcaa tcttgtaaaa ttcctagcaa
     5641 aaaagagagc caaaactctc tttttatctt cttttacttt ttttgactgg catgagtgtg
     5701 atgtctctaa cactaaagta agctaggatc aacatggcta ttgctaggaa tatttctgtt
     5761 ggtaattgaa aaattttcag aaaagataga accaataaaa tcaagagtgc cactaaaata
     5821 cataccatag cgacgatatt gacagtccct ttaatgcttt ctggtgtcgc aaatacatag
     5881 agtaggagca gtaaaattcc taggactaaa tagaccatct ttctctcttt ctagctctta
     5941 ttcagctgat tttttcttct tgttagcttt ctcacgctct gctttgttaa ggatttgttt
     6001 acgcaaacgg atagactcag gcgttacttc catgtactca tcgtcgttca agaactcaag
     6061 agactcttca agtgtcaaga tacgaggcgt cttgataaca gctgtttggt ccttagtagc
     6121 tgaacgaacg ttggtcattt gttttgcctt cgtgatgtta actgtcaagt cattttcacg
     6181 agagttttca ccgatgatca ttccttcata aacctcagta cctgggttga caaagatcgt
     6241 accacgttct tcgatagaca tgattgagta agttgtagcc ttaccagcat cgatagaaac
     6301 aagggcacca cggtgacgtc caccaatttc ccctggaatc aatggcaagt attggtcgaa
     6361 ggtatggttc atgataccgt aaccacgagt cattgacaag aactcagttg agtatccaat
     6421 caaaccacgc gctggaacaa ggaagaccaa acgagtttga ccattaccag ttgaaatcat
     6481 atccaacatt tcacctttac gttcagaaag gctttggata acagaccctt ggtattcttc
     6541 tggagtgtcg atttgtacac gttcaaatgg ttcacattta acaccgtcga tttcttttac
     6601 gataacttct ggacgagata cttgaagttc atagccctca cgacgcattg tttcgataag
     6661 gattgacaag tgcaattctc cacgtcctga aacagtccat ttatctggtg aatcagttgg
     6721 gtcaacacga agggaaacgt ctgtttgcaa ttctgcctgc aagcgttctt ccaccttacg
     6781 agaagttacc catttacctt ctttaccagc aaatggtgag ttgttgacca agaaagtcat
     6841 ttgaagagtt ggctcatcga tgtgtaggat tggaagagct tctactgcat ctgtcggagt
     6901 gatggtttca ccgacaaaga tgtcttccat acctgaaacg gcaatcaagt cacccgcttt
     6961 ggcttcttgg atttcacgac gttccaaacc aaagaaaccg aagagttttg taacacggaa
     7021 gtttttagtt gtaccgtcaa gtttagaaag ggtaacctgg tccccaacct taactgtacc
     7081 acggaagaca cgaccgatac cgatacgtcc aacaaagtca ttgtagtcca aaagtgacac
     7141 ttggaactgc aaaggctcat ctgagttatc tactggagct gggatatggt cgataatcgt
     7201 gtcaaagatt ggtgccatag tcgcttcttg gtcagctgga tcatctgaca atgaagaagt
     7261 tccgttgatc gctgaagcat aaaccactgg gaaatcaagc tggtcgtcat ctgcaccaag
     7321 ctcgatgaaa agttccaaga cttcatccac tacttctgct ggacgagctg atggcttatc
     7381 gattttgtta acaaccacga ttgggacaag gtcttgttcc aaggcttttt tcaatacgaa
     7441 acgagtttgt ggcatggttc cttcataggc atctacgacc aagacaacac cgtcaaccat
     7501 tttcatgata cgctcaactt ctccaccaaa gtccgcgtgt cctggtgtgt ccataatgtt
     7561 gatacgagtt ccgttgtaag caacggcagt atttttagca aggatggtaa tcccacgctc
     7621 tttttcgata tcgtttgagt ccatagcacg ctctgccaat tcagtacgtg catcaagcgt
     7681 ttctgattgt ttcaataatt cgtcaaccag ggttgtttta ccgtggtcaa cgtgggcgat
     7741 aatcgcaatg ttacggatat cttctcttaa ttttgtcatg atttcctcta taatattcaa
     7801 aatttatttt ctaactgaac gattatacca taatttcaaa taaataacat aactcaagca
     7861 agtgtaaatg ttttcactcc acttttcttt tcacgtcaag ccttttcaaa gcaagcgact
     7921 tatgataaga taggcacagt atgcgtttag ataatttatt agctcaagaa aaaatcagcc
     7981 gaaaggccat gaaacaagcc ttactcaaag gggacattct agtcgatggt tgcccagccc
     8041 gctccctagc tcaaaatatc gatacaggac tacaagaact cctttttcag gatcgaatca
     8101 ttcaaggcta tgaacacacc tatcttatgc ttcataaacc tgctggtgtc gttacagcca
     8161 acaaagacaa ggaacttccg accgtcatgg acttgcttcc atctgacatc cagtctgaca
     8221 agctctatgc cgtcggccga ctggaccgag atacaacagg actccttctc ttgaccgata
     8281 acggtccctt gggcttccaa ctcctccatc cccaatatca tgtcgataag acttatcaag
     8341 ttgaggttaa tggacttcta acacctgacc atatccaagc ctttcaaaag ggaattgtct
     8401 ttttagatgg tactgtctgt aaacccgcaa aactagagat tctatctgca agtccttccc
     8461 tcagtcaagc ctctatcacc atttcagaag gaaaatttca tcaaatcaag aaaatgttcc
     8521 tctcggttgg tgttaaggta actagcctca agagaatcca atttggggac ttcacattga
     8581 acccagattt agcagaaggt aactaccgcc ctttgaacca aaaagagtta caaatcatta
     8641 aaaactattt agagatgagt cgataaaata aaaaaagctt taaaactaaa gcttttttct
     8701 tttatttacc gaaaaattaa ggcgattgct acaatccagt taactacaga aatcacaatt
     8761 cctaagatat taagaatctt ttctatttta tagtctaatt gtgactcttt ttggtatgaa
     8821 atagccaaga ccaatcctat gatacccaaa atcaggccta caattggaaa taacaaacca
     8881 agaataatcg acaagatacc cacaaaaagt ggatttttct tcttttcttt tatgttctaa
     8941 gaactcctta aattttatac aaattaatta tactataaaa caatagcttc atcctatcat
     9001 tcgactaatt tggaaataag gttagctagt cttcactttc cctttccaag aatccaagcc
     9061 ataagaaagg atataaatct cagaaaaacc ttgttttttc aagtaaagag ctgcatttgt
     9121 aactcgttgc gcacgttggt tttcgtagag aaggacaggt ttatctttac gaagggctgc
     9181 aagactagtt ttcaactgac ttgaaggaat attgcgtgca ccaaggatat gttttctgtg
     9241 gaattctgct gggtcgcgca aatcaatcaa ttgacccgta cgaatcaagg cttcaaactc
     9301 ctcattgtcc acaattttag ccgcacggcg aatacgaaga tagttaaagc ccatccacgc
     9361 caacattgct agtataagtg cccacaaaat ccaagtaacc attagttctt ttctccattt
     9421 ttctcaatat aatccaattc taccttgtgc tctctgcgaa gaactgcttc tgcctctaga
     9481 tagtctaatt tatccatcaa ccctgcatcg taaatccgag atagttccaa cttcatcagt
     9541 tcaatatcat ataagcgttt tcccatgtaa acaataatac caaatcgttt gaggaattgc
     9601 tgcacatcat agaatgtttt cataagactc attctagcaa aattttgtgt ttttttcaag
     9661 aagagactca cacaatgctc cttattttcc tatcttcttt agcgattcta aggcaagtat
     9721 ggtacaataa aaacatgaga attcaacaat tacattatat tatcaaaatc gtcgaaactg
     9781 gctccatgaa tgaggcagcc aagcaactct ttatcactca gccaagtctc tccaatgcag
     9841 tgcgagattt ggaaaatgaa atgggcattg agatctttat ccgcaatccc aagggaatca
     9901 ccttgacccg tgatgggatg gagtttctct cttatgcccg tcaggttgtc gagcagaccc
     9961 agcttctgga ggaacgctat aaaaatcctg tcgcccaccg cgaactcttt agcgtttcgt
    10021 ctcaacacta tgcctttgtg gtcaatgcct ttgtctcttt gctcaagaaa agcgatatgg
    10081 agaaatacga actcttcctt cgtgaaactc ggacttggga gattatcgac gacgtcaaga
    10141 acttccgcag tgaggtcggg gtcctcttct taaacagtta caaccgtgat gttttaacca
    10201 agatgctgga tgacaatcac ctgctatccc accatctctt cacagcgcaa ccgcatatct
    10261 ttgtcagcaa gaccaaccct ctggcaaaga aagacaaggt
//
