LOCUS       NZ_FIXU01000027         9927 bp    DNA     linear   CON 15-DEC-2024
DEFINITION  Streptococcus pneumoniae strain B18210a, whole genome shotgun
            sequence.
ACCESSION   NZ_FIXU01000027
VERSION     NZ_FIXU01000027.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2016) SC, Wellcome Trust Sanger Institute, CB10
            1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900052895.1-RS_2024_12_13
            Annotation Date                   :: 12/13/2024 02:58:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,246
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 62 of 158
            Pseudo Genes (incomplete)         :: 91 of 158
            Pseudo Genes (internal stop)      :: 44 of 158
            Pseudo Genes (multiple problems)  :: 32 of 158
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:33:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 17400
            Orig. end                         :: 27327
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FIXU01000027.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..9927
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..9927
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..9927
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5252](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5252
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(792..1094)
                     /locus_tag="A1M11_RS13670"
                     /old_locus_tag="ERS050419_02079"
     CDS             complement(792..1094)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A1M11_RS13670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="ERS050419_02079"
                     /product="hypothetical protein"
                     /protein_id="WP_061649920.1"
                     /transl_table=11
                     /translation="MKIKEQTRKLAAGCSKHCFEVADRTDEVSSKHCFEVVDGTDEVSS
                     KHCFKVVDGTDKVSSKHCFEVVDRTDEVSSKHCFEVVDRTDEVSNHIRQGDVDVV"
     gene            complement(1144..1776)
                     /locus_tag="A1M11_RS10575"
                     /old_locus_tag="ERS050419_02080"
     CDS             complement(1144..1776)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /locus_tag="A1M11_RS10575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS050419_02080"
                     /product="HAD family hydrolase"
                     /protein_id="WP_050129035.1"
                     /sec_met_domain="HAD_2 (E-value: 2.2e-39, bitscore: 126.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIRG
                     FMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPLYI
                     TTTKDTSTAQDMAKNLEIHHFFDGIYGSSPKAPHKADVIHQALQTHQLAPEQAIIIGDT
                     KFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(1798..2670)
                     /gene="sdaAA"
                     /locus_tag="A1M11_RS10580"
                     /old_locus_tag="ERS050419_02081"
     CDS             complement(1798..2670)
                     /EC_number="4.3.1.17"
                     /GO_function="GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /GO_process="GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sdaAA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /locus_tag="A1M11_RS10580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS050419_02081"
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="WP_000500038.1"
                     /transl_table=11
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLMER
                     NLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNEHN
                     AKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASISGA
                     EGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEVPCVK
                     RNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLATTPTGR
                     RLQKEIFGE"
     misc_feature    2654..2670
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 24.79"
                     /tool="antismash"
     gene            complement(2679..3350)
                     /gene="sdaAB"
                     /locus_tag="A1M11_RS10585"
                     /old_locus_tag="ERS050419_02082"
     CDS             complement(2679..3350)
                     /EC_number="4.3.1.17"
                     /GO_function="GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /GO_process="GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sdaAB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /locus_tag="A1M11_RS10585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS050419_02082"
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="WP_000838915.1"
                     /transl_table=11
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPTE
                     VEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKESN
                     APHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDIPGM
                     IALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHNVNFF
                     K"
     misc_feature    3506..3522
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 26.23"
                     /tool="antismash"
     gene            3592..4179
                     /locus_tag="A1M11_RS10590"
                     /old_locus_tag="ERS050419_02083"
     CDS             3592..4179
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /locus_tag="A1M11_RS10590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS050419_02083"
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="WP_000836442.1"
                     /transl_table=11
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLSE
                     IAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETVSA
                     PVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYS
                     AENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(4231..>4557)
                     /locus_tag="A1M11_RS10595"
                     /pseudo=""
     CDS             complement(4231..>4557)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /locus_tag="A1M11_RS10595"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="DNA-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="KILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMCKQICYT
                     LLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     misc_feature    complement(4641..4654)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.64"
                     /tool="antismash"
     misc_feature    4641..4655
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.39"
                     /tool="antismash"
     gene            5001..5252
                     /locus_tag="A1M11_RS10600"
                     /old_locus_tag="ERS050419_02084"
     CDS             5001..5252
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /locus_tag="A1M11_RS10600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS050419_02084"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_049479957.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 1e-32, bitscore:
                     103.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVCSILASTSVLAVWVDGGQWNYGVGWSGNFGYSDYLHSTRSHTA
                     TVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
     gene            5304..7412
                     /locus_tag="A1M11_RS10605"
                     /old_locus_tag="ERS050419_02085"
     CDS             5304..7412
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /locus_tag="A1M11_RS10605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS050419_02085"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_000724257.1"
                     /transl_table=11
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDLY
                     LKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLDFT
                     TDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEMDKD
                     RIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQPMMEM
                     KRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPMLWLSHF
                     LLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTALLIGVGR
                     SLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFYQDLVEKT
                     QAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTGIKKVILMP
                     ASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFPYSDKRKENL
                     SNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKLSDGNYLKFSS
                     IQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRDIITKKFMGWKL
                     VDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVLGLASRMEKRSLV
                     ELLKGGIL"
     gene            7409..8050
                     /locus_tag="A1M11_RS10610"
                     /old_locus_tag="ERS050419_02086"
     CDS             7409..8050
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /locus_tag="A1M11_RS10610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS050419_02086"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000571329.1"
                     /transl_table=11
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLLN
                     LLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLMLAL
                     KYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLILADE
                     PTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRIIELGEGK"
     misc_feature    8235..8255
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 26.44"
                     /tool="antismash"
     gene            8256..9062
                     /locus_tag="A1M11_RS10615"
                     /old_locus_tag="ERS050419_02087"
     CDS             8256..9062
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /locus_tag="A1M11_RS10615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS050419_02087"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_001038481.1"
                     /transl_table=11
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVALN
                     PDFAPFEYQKVVDGKNQIVGSDIELAKAIATKLGVELELSPMSFDNVLASVQSGKADLA
                     ISGVSKTDERSKVFDFSIPYYTAKNKLIVKKSDLTTYQSVNDLAQKKVGAQKGSIQETM
                     AKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVSKGFVENNPDLAIADLNFEKEQ
                     DDSYAVAMKKDSKKLKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            9079..>9471
                     /locus_tag="A1M11_RS10620"
                     /old_locus_tag="ERS050419_02088"
                     /pseudo=""
     CDS             9079..>9471
                     /GO_function="GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269502.1"
                     /locus_tag="A1M11_RS10620"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ERS050419_02088"
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSKEKVILAYSGGLDTSVAITWLKKDYDVVSVCMDVGEGKDLDFI
                     HDKALKVGAVESYVIDVKDEFATDYVLVAHQSHAYYEQKYPLVSALSRPLISKKLVEIA
                     HQIGATTIAHGCTGKGNDQVEYQIAVA"
     gene            complement(9787..>9927)
                     /locus_tag="A1M11_RS10625"
     CDS             complement(9787..>9927)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /locus_tag="A1M11_RS10625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transposase"
                     /protein_id="WP_137995553.1"
                     /transl_table=11
                     /translation="LEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSR
                     A"
ORIGIN
        1 gatggagcgc aatccaaaag aagccttcaa aaacaatatc cgtggaactt acaatgttgc
       61 tagagccgtt gatgaagcca aggtgcctaa gatggttatg atttcgacag ataaggcagt
      121 taatccacca aatgttatgg gagcaaccaa gcgcgtggcg gagttgattg tcactggctt
      181 taaccaacgt agccaatcaa cctactgtgc agttcgtttt gggaatgttc ttggtagccg
      241 tggtagtgtc attccagtct ttgaacgtca gattgctgaa ggtgggcctg taacggtgac
      301 agacttccgc atgacccgtt actttatgac cattccagaa gctagccgtc tggttatcca
      361 tgctggtgct tatgccaaag atggggaagt ctttatcctt gatatgggca aaccagtcaa
      421 gatttatgac ttggccaaga agatggtgct tctaagtggc cacactgaaa gtgaaattcc
      481 aatcgttgaa gttggaatcc gcccaggtga aaaactctac gaagaactct tggtatcaac
      541 cgaactcgtt gacaatcaag ttatggataa gattttcgtt ggtaaggtta atgtcatgcc
      601 tttagaatcc atcaatcaaa agattggaga gttccgcact ctcagtggag atgagttgaa
      661 gcaagctatt atcgcctttg ctaatcaaac aacccacatt gaataaaaaa gaaaaacgca
      721 tagtatcaag tttgttaacc ttggtactat gcgttttatt atgtagagac ttatactctt
      781 cgaaaatctc ttcaaaccac gtcaacgtcg ccttgccgta tatggttact gacttcgtca
      841 gttctatcca caacctcaaa acagtgttta gagctgactt cgtcagttct atccacaacc
      901 tcaaaacagt gtttagagct gactttgtca gttccatcta caaccttaaa acagtgttta
      961 gagctgactt cgtcagttcc atctacaacc tcaaaacagt gtttagagct gacttcgtca
     1021 gttctatctg caacctcaaa acagtgttta gagcagcccg cagctagttt cctagtttgc
     1081 tctttgattt tcattgagta ttacttcatt ttcttctgaa atggaattgt tacccagtct
     1141 atgctattga aaatacgcca aaacttctaa gggtttgtga gcgatataat caggttgata
     1201 gtttagtaga tctgcttgct ctccaaatcc ccaagtgatg gccaatttct gaatacctgt
     1261 ttctcgagct cccagcatat caaacttggt atctccgatg atgatggctt gttctggtgc
     1321 tagttgatgt gtctgcaagg cttggtgaat gacatctgcc ttatggggtg ctttagggct
     1381 agaaccataa atgccatcaa agaaatgatg gatttccaag ttttttgcca tgtcttgagc
     1441 agtagatgta tcctttgtcg tggtgatgta gagtggataa ctgctcgata actcctcaag
     1501 caagtctata atctgaggaa agagttgagc ttcatagatg ccttttgcct tatagtaaga
     1561 acgatatatc tgcacggctt cagaaatttg gtctttggac aggcaggtcg caaaactact
     1621 ttcgagaggt ggtcccataa aaccacgaat agttttggca tcagggctag gcacccccag
     1681 ctctttaaag gtataggtaa aggcattgtg aatcccgata gaactatcaa cgagggttcc
     1741 atccaaatcg aaaaaaatcg ctgtgataga ggtcatggtt tctcctattt gataagctta
     1801 ttctccgaaa atttcttttt ggaggcgacg accagtaggg gtggtagcga gtccaccttc
     1861 agctgtttca cgaaaggcag ttggcatgct tgctcctact tggtacatgg catcgatcac
     1921 ttcatccaca gggattttag attcgatacc tgccaaggcc atgtctgctg cgatgaaagc
     1981 aaagctagct cccatggcat tacgtttgac acagggaact tcgaccaaac ctgcaacagg
     2041 gtcacagatg aggcctagca tatttttaat gacaaaggca atagcttgac tggcctgata
     2101 aggtgttcca cctgcagcca gagtcaaggc ggcagcactc atagcagagg ctgaaccaac
     2161 ttcagcttga cacccaccct cagcacctga gatggaggca ttgtttgcga tgactagtcc
     2221 aaaggcacca gcagcaaaga ggaaatccaa ttgttgctcg tggctgaggt ctaatttttc
     2281 aatagcagca gtgagaacgg atggcagaca gccagcactt ccagcggttg gagtggcaca
     2341 gaccaagccc attttggcat tgtgttcatt gactgcgatg gcatttcggg cagccgagag
     2401 aatcgtataa tctgacagag tttttccgtt ttcgatgtag tgatccaatt tggcagcatc
     2461 tccacctgtc aggccactac gagatttatt ttcattgagg ccaagttgga cagaggcttt
     2521 cataacttcc agattgcgtt ccatgagaag gaagacttct tcacgttcgc gaccggtcaa
     2581 ttcaaactct gttgtaatca tgagttctgc gacatttcct tgaaagtcca gatctgcttg
     2641 ctcgaccaat tctttgatag aataaaacat gcttcctcct atttaaagaa attgacattg
     2701 tggagatgag ggatttttcg gatttcttcg atagcctcat cacagttgcg actgtcaact
     2761 tcgataatca taatggcttt ttcaccagct ttttcacgag tgacattcat ctgggcgata
     2821 ttgataccat agcgggaaag cgcctctgta acaagggcaa tcatacctgg aatatcttga
     2881 tgaacgatga tgatagtcgg tgtattcata ttgagagaga cggcaaaacc attgagttcg
     2941 gttacctgaa tatttcctcc accgatagaa ataccagtca cgctgatggt cttgtgggca
     3001 tttttaacag taattttagt ggtgttaggg tgaggggcat tgctttcttt ctgaatggtc
     3061 cagacaatct tgataccacg cttgtgggca atttccagac tatttggaat ttcaggatca
     3121 tctgtatcca tgcctaaaat acctgcaaca agggctaggt ctgttccgtg accacgatag
     3181 gtcttggcaa atgagttaaa aagttggaat tcaacttctg tcggagtatc atcaaaaatg
     3241 gaagagacaa tcttcccaat acgaacagca ccagcggtat ggctactaga tgggccaatc
     3301 ataactggtc cgatgatatc aaagacagat tgaaaacgaa gtgatttcat cagtttcccc
     3361 ttataaaaat tcttatctct attatatcaa agaatgaggg gcttggcttt aattgtggat
     3421 gaaaaccttt ctaatacctc aaatagcata aaaatagtat cttttatgac aaaaaacacc
     3481 ttatttaggg aaataaaaaa taattttgta atatttctac ataaaagtgt caagaaacgg
     3541 taatatttaa agggtatgat agaactatag aaagaaggag aattttcgaa tatgaaatca
     3601 ataactaaaa agattaaagc aactcttgca ggagtagctg ccttgtttgc agtatttgct
     3661 ccatcatttg tatctgctca agaatcatca acttacactg ttaaagaagg tgatacactt
     3721 tcagaaatcg ctgaaactca caacacaaca gttgaaaaat tggcagaaaa caaccacatt
     3781 gataacattc atttgattta tgttgatcaa gagttggtta tcgatggccc tgtagcgcct
     3841 gttgcaacac cagcgccagc tacttatgcg gcaccagccg ctcaagatga aactgtttca
     3901 gctccagtag cagaaactcc agtagtaagt gaaacagttg tttcaactgt aagcggatct
     3961 gaagcagaag ccaaagaatg gatcgctcaa aaagaatcag gtggtagcta tacagctaca
     4021 aatggacgtt atatcggacg ttaccaatta acagattcat acctgaacgg tgactactca
     4081 gctgaaaacc aagaacgtgt agcagatgcc tacgttgcag gacgttacgg ttcatggact
     4141 gctgctaaaa acttttggct taacaatggc tggtattaag aactaacgaa caaataatat
     4201 gtaaagagtc gaggaaatcc ctcggctttt ttatctttcc ttcgcttggt aatagatctc
     4261 tacttctttt ttgagatgag acagcataga agtttcctcg gttagctcca gaagggagaa
     4321 ccctttttgg ataagtttag aatcgtctgt acaaatccca atacggacat agcagatagc
     4381 cagcctatcg tagcttttct tattcttggc gtcctctagt aaagtgtagc agatttgctt
     4441 acacatgttt ttatcttgat tgtatagata gagtgtggaa aggtttaata gaattgtcat
     4501 tcgcaagtca tataaatgtt gatagttttt atagacttct aggcgttgca agattttctc
     4561 ggattattga caaataaacg gacattctga tagacgattg ccctagcggc ttattgtaaa
     4621 ctttgcaaat ttggcttgta attttttata aaattcactt caattacttg acaattatta
     4681 tattttcact tattatatag ataaaaggac gggataggag tttaaaaatt tgaaagcgct
     4741 atccatattg tttattgggc tgattcttga gtaatagtta gagtgtgatt aatactactc
     4801 tggttgttca aagacctgat acagaagaga actattctcc tagttaagag taagttcgtt
     4861 gtttaaaaat gaaatgagat agaaggaagg tgagtccaat ggactacgat acttgttgac
     4921 aaattactat agctgtttct acggaatagc tctcgataat aggagaaata aatcatgaga
     4981 aaattagtgt tgcatcttta atggtatgta gtatacttgc ttctacaagt gttttggcag
     5041 tatgggttga tggtggtcaa tggaactatg gagtaggatg gtcaggaaat tttggttatt
     5101 ctgattattt acattctact cgctctcata cagcaactgt tagacatggg ggtagaacct
     5161 ctaaggatta tgcaaaacct gaggcatggg ctagagcttc cctcaccaag attccgccaa
     5221 caggaatgga atatttctat ggatttgagt aaaattcttc cctctcctat ttggagaggg
     5281 aaaactgtag tggaggcact tttatgaaaa aaatcagtaa tttctgtatg ttactcctgc
     5341 ttctgtgtac cacttttttt gtttttaatg taaactatac acgagaagtg gttcggattc
     5401 aagaaatggg aaagactgta gattctttgg atttgtattt gaaagatatt aacgaacctg
     5461 cagcgtctgt tcttcgattt tttgaggatg tatcaaagga gtataaagtc tccatcatca
     5521 aaacagacag tggtgatgag gtggtcaagt ctggtgtttt tgataaagat accttcccct
     5581 accaagagtt tgggatttct tctcttgatt ttaccacaga tggtgaagga gtctatagta
     5641 ataaagaaat ttccaataaa cttggtacga ttccgacctt tctaaaagcc aaacctattc
     5701 agcttatgac ttttcaaacc tatatcaagg atacatctcg tagtttaaat ggtcgctata
     5761 cgataacttc tacacaagag atggacaagg ataggattgt acagaaatgg agcgattttt
     5821 tcaagataga ccaggctacc ttgctagagc cgacctacaa aagtgcagtg gaagtcataa
     5881 atcgagattt gcttttatct gccattgttt ttgtcttggc tattttgctt cttgtgttag
     5941 tgacagtgta tcaaccgatg atggagatga aaagagttgg ggtacaaaaa ttacttggtt
     6001 ttcaagatag ggctgtttta gctgatgttg taaaaggcaa cctttacctc ctcctaggtg
     6061 gggctcttgt gatcaatcta ggcgtgtttt tcttgcttga ttataagcca aaagatttgt
     6121 ttcctatgct gtggttgtct cattttttgc tgttgcagct ttatctcttt atcagttggt
     6181 tgacttacct cttaatccaa aaaatgacaa tcagctctct gctgaaaggt ttttcatctt
     6241 tcaaatttgg tcttatcttc aattatgtga tgaaaatagg gacaactatt ttactgacgg
     6301 ccttactgat tggggtgggc agaagtttag aacaagaaaa caaagaactt gcttatcagc
     6361 aacagtgggt aagtcaaggt aattacctga ccttagaaac cttcaaactc aatgataatc
     6421 tgtggcaaga agagctagca gggtcaggga aatctacaga ttatttctat cgattttatc
     6481 aggatttggt agaaaaaacg caggcgggct atgtgcagag tagcagtctt cctgtaaaaa
     6541 attttgtcca atcagaacag attcagcaat atcagttaac agatacggtg gatgtttact
     6601 atgccaatcg caattttcta aagagcaagg gattcaagct accagatacc ggtattaaaa
     6661 aagttatttt gatgccagca agtacgaaag gtgaagaaga taaaaatcag ctcttgggga
     6721 agttaattgc ctttcattcg atgaagtatg aagagcagca aaaacgaacg atagaggaga
     6781 tggatgtcga gattgcctat tatgaaggag attggtcatt tttcccatat agtgataagc
     6841 gaaaggaaaa tctctccaat ccaattatta gcttggtcaa tgattctgat atgatgtggg
     6901 atgagaaagc ctccctgtca acaactggct taaataatcc gattaaaatt gaaaatacgg
     6961 ttcaacatca aaaagagatt acagagttag ttgagaaatt gtcagatgga aattatttaa
     7021 aattttcatc tattcaagcc attcaacaag agaaagtgga ttcttatcga gatgctgttc
     7081 ggaattttaa cctactcttt gctttgtttg gtctccttag catgatgatt tcctacttct
     7141 tactagtaac aactttctta ttgaagcgca gggatatcat taccaagaag tttatggggt
     7201 ggaaactggt cgatcgctac cgtcctctcc tcgttctgct cttgctgggc tatagtttcc
     7261 ctcttctagt cttgattttc tttgcccatg cgttcttacc acttctactg tttgcaggtt
     7321 ttacatgtct ggatatacta tttgtgctag gcttagcttc taggatggag aaaagaagtc
     7381 tagtagagtt attgaaaggg ggcatcttat gattgagttg aaaaatatta ccaaaaccat
     7441 tgggggaaaa gtgattttgg ataacttatc tctcaggatt gatcaggggg atttggtagc
     7501 tattgttggt aagagtggta gtgggaagtc gaccttgtta aatttattgg gtttgataga
     7561 tggtgattat agcggacggt atgagatttt tggtcagaca aatctagcgg ttaattctgc
     7621 taagtcgcaa acaataatcc gtgaacatat ctcttatctg tttcaaaatt ttgccctgat
     7681 tgatgatgaa acggtcgagt acaatctcat gctggcgctg aaatatgtga aattgcctaa
     7741 gaaagacaag ctcaaaaagg tggaagagat tttagagaga gtaggtttgt cagctacttt
     7801 gcatcaaagg gtctccgagt tgtctggggg cgaacaacaa cgaattgcag ttgctagagc
     7861 catcttaaaa cccagccagc tgattttagc cgatgaacct acaggttcgc tggatcctga
     7921 aaatagagat ttggtcttga agtttctctt agagatgaat cgagaaggga aaacagtcat
     7981 tattgtgacc cacgatgctt atgtagccca acaatgtcat cgaatcattg aattaggcga
     8041 gggaaaatga gttcgttcag cttcatttga ccgactgaat actcatattt ttcagagaga
     8101 aatagcataa ataggcctag gaatgatatt tttatgtcat tttcctgggt ttttctgttt
     8161 taaattgaaa aaattacaat ttaggcttga caaaggatgc atatagatgt attgtttata
     8221 caaataaaag gaataaacat aaagtaggct ttattatgaa taagatgaag aaggtgttga
     8281 tgacgatgtt tggtttagtg atgctccccc tactatttgc ttgtagcaac aatcaatcgg
     8341 ctggaattga agccatcaag tccaaaggaa aattggttgt agccctcaat ccggattttg
     8401 ctccatttga ataccaaaaa gtggttgatg ggaaaaatca gattgtgggt tcagatatcg
     8461 aattagccaa ggctatcgca acaaaactag gtgtcgaatt ggaactatct cccatgagtt
     8521 ttgataatgt actggctagt gttcaatcag gaaaagccga ccttgccata tcaggtgttt
     8581 ctaagacaga tgaacggagc aaggtgtttg acttttccat tccctactat actgcaaaaa
     8641 ataaactcat tgtcaaaaaa tctgacttga ctacttatca gtctgtaaac gacttggcgc
     8701 agaaaaaggt tggagcgcag aaaggttcga ttcaagagac gatggcgaaa gatttgctac
     8761 aaaattcttc cctcgtatct ctgcctaaaa atgggaattt aatcacagat ttaaaatcag
     8821 gacaagtgga tgccgttatc tttgaagaac ctgtttccaa gggatttgtg gaaaataatc
     8881 ctgatttagc aatcgcagac ctcaattttg aaaaagagca agatgattcc tacgcggtag
     8941 ccatgaaaaa agatagcaag aaattgaaag aggcagtcga taaaaccatt caaaagttga
     9001 aggagtctgg ggaattagac aaactcattg aggatgcctt taaagcatcc attgaaaaat
     9061 agaaagaagg aaaagaacat gagtaaagaa aaagtaattt tggcctattc tggcggtttg
     9121 gacacatcag ttgctattac ctggttaaag aaagactatg atgttgtttc agtttgtatg
     9181 gatgtgggtg aagggaaaga cttagatttc atccatgata aggctcttaa ggttggggca
     9241 gtagaatctt atgtcattga tgttaaggac gaatttgcta cagattatgt gctagtggct
     9301 catcagtcac atgcctatta tgaacaaaag tatcccttgg tatctgcctt gagccgccct
     9361 ctgatttcta aaaaattggt tgaaatagcg catcagatag gagcgactac aattgctcat
     9421 ggttgtaccg gtaaggggaa tgaccaagtc gaataccaga ttgcagtcgc taagaaagcg
     9481 aatgaagcta aaaagtgatg gagtgatggg ttcagaataa gaggttggtc gattggcagc
     9541 ctctttcttg tcgttgaaaa agtgagatat attgactttt gaaaaaaatg tcataattga
     9601 gttgggataa acgtaaaagt catactttct cgttaaatcg ttcggcgatt catggtgaca
     9661 tggacacata gtagtaaaca cactatgtgg cttggcagag ctaaaaactt ctggtttaac
     9721 aatggctggt attaagaact aacgaacaaa taaggctctt tgtcaactgt agtgggttga
     9781 aaaaagctaa gctcgagaaa ggacaaattt cgtcctttct tttttgatgt tcaaagcgat
     9841 aaaaatccgt tttttgaagt tttcaaagtt tcgaaaacca aaggcattgc gcttgataag
     9901 tttgatgaga ttattggtcg cttccag
//
