LOCUS       NZ_FIXU01000011        10297 bp    DNA     linear   CON 15-DEC-2024
DEFINITION  Streptococcus pneumoniae strain B18210a, whole genome shotgun
            sequence.
ACCESSION   NZ_FIXU01000011
VERSION     NZ_FIXU01000011.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2016) SC, Wellcome Trust Sanger Institute, CB10
            1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900052895.1-RS_2024_12_13
            Annotation Date                   :: 12/13/2024 02:58:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,298
            CDSs (total)                      :: 2,246
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 62 of 158
            Pseudo Genes (incomplete)         :: 91 of 158
            Pseudo Genes (internal stop)      :: 44 of 158
            Pseudo Genes (multiple problems)  :: 32 of 158
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:33:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 10923
            Orig. end                         :: 21220
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FIXU01000011.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10297
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10297
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10297
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5297](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5297
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            778..912
                     /gene="rpmH"
                     /locus_tag="A1M11_RS06215"
                     /old_locus_tag="ERS050419_01208"
     CDS             778..912
                     /GO_component="GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA]"
                     /GO_component="GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA]"
                     /GO_function="GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rpmH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /locus_tag="A1M11_RS06215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS050419_01208"
                     /product="50S ribosomal protein L34"
                     /protein_id="WP_000831905.1"
                     /transl_table=11
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA"
     gene            <1118..2071
                     /locus_tag="A1M11_RS06220"
                     /old_locus_tag="ERS050419_01209"
                     /pseudo=""
     CDS             <1118..2071
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_367670414.1"
                     /locus_tag="A1M11_RS06220"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ERS050419_01209"
                     /product="DUF1542 domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="KVEVAGDGTATIVFKDGSAITIPGNQLVAQDPKAQDSTKLTAEKS
                     TVKAPAQRVDVKDITHLTDEEKEKVKVAILQANGSALDGATINVAGDGTATITFPDGSV
                     VTILGKDTVQQSAKGESVTQEATPGGDKGGNTGSSDANANAGGGSQAGGPAHTGSQNSA
                     QSQASKQLATEKESTKNAIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIE
                     NAKTMEDVKEAETIGVQAIAMVTVPKRPVAPNAAPKTTSASQATAGTMQDVTYQSPAGK
                     QLPNTGSASSAALASLGLVVATSGFALLGRKTRRRK"
     gene            2377..3150
                     /locus_tag="A1M11_RS06225"
                     /old_locus_tag="ERS050419_01210"
     CDS             2377..3150
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /locus_tag="A1M11_RS06225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS050419_01210"
                     /product="TatD family hydrolase"
                     /protein_id="WP_000575607.1"
                     /transl_table=11
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIEH
                     ALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTAPK
                     EVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLEWAE
                     KFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENKTAYT
                     RYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            3150..3710
                     /gene="rnmV"
                     /locus_tag="A1M11_RS06230"
                     /old_locus_tag="ERS050419_01211"
     CDS             3150..3710
                     /EC_number="3.1.26.8"
                     /GO_function="GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="rnmV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /locus_tag="A1M11_RS06230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS050419_01211"
                     /product="ribonuclease M5"
                     /protein_id="WP_050089555.1"
                     /transl_table=11
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERIQ
                     RLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEHAS
                     YEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSCKRREYLGETLRIGYSNGKQ
                     LLKRLELFGVTLAEVEEAMKSYE"
     gene            3782..4681
                     /locus_tag="A1M11_RS06235"
                     /old_locus_tag="ERS050419_01212"
     CDS             3782..4681
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /locus_tag="A1M11_RS06235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS050419_01212"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000904862.1"
                     /transl_table=11
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPSL
                     PKLDYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEIYK
                     TYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEVYSTNDMLLIRL
                     FFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVGVTDC
                     FNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAQLIGDE
                     FLVKKLTEEWQEDVKKYL"
     misc_feature    4752..4761
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     misc_feature    4838..4851
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 22.05"
                     /tool="antismash"
     gene            5001..5297
                     /locus_tag="A1M11_RS06240"
                     /old_locus_tag="ERS050419_01213"
     CDS             5001..5297
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /locus_tag="A1M11_RS06240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS050419_01213"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000815394.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 2.5e-21,
                     bitscore: 68.6, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQTVKKLALVASIAATLGGSVAVASAAVQYPEGGVWTYGSGNGG
                     AYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            5374..7404
                     /locus_tag="A1M11_RS06245"
                     /old_locus_tag="ERS050419_01214"
     CDS             5374..7404
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /locus_tag="A1M11_RS06245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS050419_01214"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_061747130.1"
                     /transl_table=11
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMTNQ
                     PVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKFPEGLTISSKES
                     AETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLLSLA
                     IFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSLLGLG
                     ILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGKLPLKR
                     MMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGWSSAFAD
                     EEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTPSGNVIYV
                     SPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSIYYQGLFTDYLQNFSSESV
                     EVTSQKHYLPQVRLAFTEIGQERFLYNDGYKTTRQYLKDPIIVVLTPQATGTRPVAGML
                     WGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRVEFYSLLIGTI
                     LTLSTAILLFDSMNLLYFEQFRRDLMIKRLAGMTIYELHGKYLLAQGGVLLLGLVLSSI
                     LTRDGLISALVVALFTLNTLFILVRQDKKEEAGSMAVLKGK"
     gene            7406..8047
                     /locus_tag="A1M11_RS06250"
                     /old_locus_tag="ERS050419_01215"
     CDS             7406..8047
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /locus_tag="A1M11_RS06250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS050419_01215"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000565531.1"
                     /transl_table=11
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLLN
                     ILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFVGQ
                     KISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILADEPT
                     AALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            8040..8348
                     /locus_tag="A1M11_RS06255"
                     /old_locus_tag="ERS050419_01216"
                     /pseudo=""
     CDS             8040..8348
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /locus_tag="A1M11_RS06255"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ERS050419_01216"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MCEKIRIRRVSDYPSARGGL"
     gene            8479..9351
                     /gene="rsmA"
                     /locus_tag="A1M11_RS06260"
                     /old_locus_tag="ERS050419_01217"
     CDS             8479..9351
                     /EC_number="2.1.1.182"
                     /GO_function="GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="rsmA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /locus_tag="A1M11_RS06260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS050419_01217"
                     /product="16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="WP_001216856.1"
                     /transl_table=11
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEID
                     DQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDILKV
                     DLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRISAQ
                     PNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVEDENFF
                     FKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLAEFAGL
                     ADALKGQGL"
     gene            9353..10231
                     /gene="rsgA"
                     /locus_tag="A1M11_RS06265"
                     /old_locus_tag="ERS050419_01218"
     CDS             9353..10231
                     /GO_function="GO:0003924 - GTPase activity [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_function="GO:0043022 - ribosome binding [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rsgA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /locus_tag="A1M11_RS06265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS050419_01218"
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="WP_001161981.1"
                     /transl_table=11
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVDF
                     SAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHKGI
                     HPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGVGKS
                     TLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVSRAED
                     LNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEIENRRE
                     TYKKVSKKIPK"
ORIGIN
        1 gagattgccc gtcaaaacga tttgattatc tttgcggatg aaatctatga ccgcatggta
       61 atggacggac atgtgcatac gcctgtggcg agcttggcac cagatgtctt ctgtgtcagc
      121 atgaatggtc tgtcaaaatc ccaccgcata gcaggtttcc gtgtgggttg gatggtcttg
      181 tctggcccta agactcatgt taagggctat atcgaagggc tcaatatgct gtccaatatg
      241 cgcctttgct ctaacgtttt ggcccaacaa gtcgtacaaa cttccttggg gggtcaccaa
      301 tcagtcgatg aattgcttct tcctggtgga cgaatctacg agcaaagaaa tttcatctat
      361 aatgccattc aagatattcc aggtttgtct gccgttaaac ccaaggcggg gctctatatc
      421 ttcccaaaaa tcgaccgcaa tatgtaccgt atcgatgatg atgagcagtt tgtccttgat
      481 ttcttgaagc aggaaaaggt tctcttggtt catggtcgag gctttaactg gcaggaacca
      541 gaccacttcc gtatcgttta ccttcctcgt gttgatgagt tagcccaaat ccaagaaaag
      601 atgactcgtt tcttgaaaca gtatcgtaga tagggcttgc attcgaaaaa gctggaaaca
      661 tttgcctaga gctattgaca aaagtgaaag aatcagatag aatagtaaag tatgtttagt
      721 aactgatcac tttatagccg gctaaacgaa tacaaaatct atgaggaggt attcatcgtg
      781 aaacgtactt atcaaccaag taaacttcgt cgtgcgcgca aacacggatt ccgtaaccgt
      841 atgtcaacta aaaatggtcg tcgcgtattg gcagctcgtc gtcgtaaagg acgcaaagtt
      901 ttggctgcat aatccaaacg aattctatca aaaatcagta ggaactcgag tctactgatt
      961 tttatttttg taaaaaagtt cagtagatgc aaatggattc ggaagcgatg ttatagtaga
     1021 ttgaaactag aatagtacac ctctatttct aaaacattgt tagaaatcga tttgactgtc
     1081 ctgatcgatt tgtcctgtta ttatttcatt ttactataaa gttgaagtag ctggagatgg
     1141 cacagcgaca atcgtcttta aagatggttc agctattaca attccaggaa atcaattggt
     1201 agcacaagat ccaaaagcac aagatagcac taaactgact gctgaaaaat caactgttaa
     1261 agcacctgct caaagagtag atgtaaaaga tataactcat ttaacagatg aagaaaaaga
     1321 aaaagttaag gttgctattt tacaagcaaa tggttcagca ttagacggag cgacaatcaa
     1381 tgtagctgga gatggtacag caacaatcac attcccagat ggttcagtag tgacgattct
     1441 aggaaaagat acagttcaac aatctgcgaa aggtgaatct gtaactcaag aagctacacc
     1501 aggtggagat aagggaggca atactggaag ctcagatgct aatgcgaatg caggcggtgg
     1561 tagccaggca ggtggaccag ctcacacagg ttcacaaaac tcagctcaat cacaagcttc
     1621 taagcaatta gctactgaaa aagaatcaac taaaaatgcc attgaaaaag cagccaagga
     1681 caagcaggat gaaatcaaag gcgcaccgct ttctgataaa gaaaaagcag aacttttagc
     1741 aagagtggaa gcagaaaaac aagcagctct caaagagatt gaaaatgcga aaactatgga
     1801 agatgtgaag gaagcagaaa cgattggagt gcaagccatt gccatggtta cagttcctaa
     1861 gagaccagtg gctcctaatg ctgctcctaa gacaacaagt gcatcgcaag caactgcagg
     1921 aacaatgcaa gatgttacct accagtcacc tgctggcaaa caattaccta acacaggttc
     1981 agcatcaagt gcagcacttg ctagtcttgg tctagtggtg gcaacaagtg gttttgcttt
     2041 gctaggaaga aagactagac gtagaaaata gaacagctag aaaattctat tctctactta
     2101 aagttagatt ataaggggga ttttgagaag tcatcaatcc tagtgatggg tgagaaaagt
     2161 gagaacccaa gataatcaca tactttagct gaataggaat attctatcaa tgtagccaat
     2221 ctcttctgtc tctaactgtg gaataggaga tgggcaatat cggatagaaa agatagcaga
     2281 atagctctct attgaagaga ggaggggaaa ccgaaaaatt aggtgcccct cctctttttt
     2341 ggtataatag aaggatagaa aacgaggtta gaagagatga tttttgatac acatacacac
     2401 ttgaatgtag aagaatttgc aggtcgtgag gcagaagaaa ttgccttggc tgctgagatg
     2461 ggtgtgacac agatgaatat tgttggtttt gataaaccga cgattgagca tgccttggag
     2521 ttggtagatg agtatgagca gctctatgcg actattggtt ggcatcctac agaagctggt
     2581 acttatacag aggaagttga ggcttacttg ttggataagt taaaacattc caaggttgtg
     2641 gctttaggtg aaattggctt agactaccat tggatgacag cgcccaaaga ggtgcaggag
     2701 caggtttttc gccgtcagat tcagctatct aaggacttgg atttgccttt tgttgtccat
     2761 acccgtgatg cgctggaaga tacctatgag attatcaaga gtgagggcgt tggtcctcgt
     2821 ggtggtatca tgcattcatt ttcagggacg cttgagtggg cagagaagtt tgtggatctt
     2881 ggtatgacca tttccttctc aggagtggtg actttcaaga aggcaactga cctccaagaa
     2941 gcagctaaag agttaccttt ggacaagatg ttggtggaaa cagatgcgcc ttacttagca
     3001 cctgtaccca agcgtggtcg tgaaaataaa acagcctata ctcgctatgt ggtcgacttt
     3061 atcgctgact tgcgtggtat gacgacagaa gagctggcgg tagcaacgac tgcaaatgca
     3121 gaacgcattt ttggattgga cagcaagtaa tgaaagagaa aatttctcaa gttatcgtgg
     3181 ttgaaggtcg cgatgatacg gtcaatctca agcgttattt cgatgtggag acctatgaga
     3241 cacgaggttc tgccatcaat gatcaggata tagagcggat tcagcgcctg caccaacgtc
     3301 atggagtcat tgtctttaca gacccagatt ttaatgggga gcggattcgg cgcatgatta
     3361 tgacggccat tccaacagtt cagcatgcct ttctcaagcg agatgaagct gttcccaagt
     3421 ccaagaccaa ggggcgttct ctgggaattg agcatgccag ctatgaagac ctgaaaacgg
     3481 ctctagctca ggtgacagaa caatttgaac atgagagtca gtttgacatt agtcgtagcg
     3541 acttgattcg ccttggtttt ctagcagggg cagacagctg taagcgtaga gaatatctcg
     3601 gagagactct ccgaatcggc tattctaacg gcaagcaact cctcaaacgc ctagagttgt
     3661 ttggggttac tttggcagaa gtggaagaag ctatgaaatc ttatgagtag gaaagatgta
     3721 gccgttacaa ttttttaagt ttcacagtat ttttcgaagc aggtagaaga ggaggcgtct
     3781 gatgttaatt ggtcaaaaaa ttaaagagat tcggatagaa aaaggaatta gtcgtccaga
     3841 tttttgtgga gatgagcaag aactgacagt tcgtcaactg tcgcgaattg aaagtggagc
     3901 ttcgcaaccg agtttgccca agttagacta tattgctcgc cggctaggag ttccagttta
     3961 tagccttatg ccggattttt cagctcttcc ttctgcttat ttagaattga aataccagat
     4021 tttacgtgaa ccaatctatg gtaaagaaga ggagtacgat aagaaggaag cgtgtttgga
     4081 agagatttat aaaacatact ttgataatct tcctaaagaa gaacaattag catgtgaagt
     4141 attgcaggcg tgtttggata cttctagaac tagaaggcct gaatatgcag agttaatact
     4201 tgaggaacat atgcctcaga tcatagaaaa agaagtttat tcaacaaatg atatgttgtt
     4261 gattcgtttg tttttttatc aaatgctcat tagaaaagat cttgccaaat ttataaatca
     4321 aatcgaaaag ctaatgctct ttcttttgga acagaaaaag gtaactaaat tagaaaactt
     4381 ctttataatt agagatactc ttatttcagg aatgtgttgt cttgaaaagg taggagtaac
     4441 tgattgtttt aatgattatc tatcgtgttt acaagaaatt atggataaaa ctcaagatta
     4501 tcaaaagaaa cctcttgtat ttatgttttt gtggaagcaa gcattaagag aagaaagaga
     4561 ttttagttta gctgaatcat tttatcagtc ttctaaaaca tttgcgcagc taattggaga
     4621 tgaatttcta gtaaagaaat tgacagagga atggcaagag gatgtcaaaa aatatttata
     4681 aacatagtga atcagtgaca aaaatgtcct tgtcctcgta tcaaaacagt tctaaagttc
     4741 gtctttagaa ctgttttttt agatataagc taaaaataat atgaaatagt tagattttaa
     4801 ggacattgat gtccttgata gtgtgataaa gatggagtat aatgactata aaagttcatt
     4861 tcagggtgct attttgacaa tttagagaaa aacaaataat tttagaatat catttggtaa
     4921 tataatagta tcaacgttga taatggaaag aggagcaagt aactcaacaa tcttgtaatc
     4981 aaaaatagag gagtatcaat atgaaacaaa cagttaaaaa attagctctt gtagcgagca
     5041 ttgcagcaac attaggtgga agtgtagcag ttgcctctgc agccgttcaa tacccagaag
     5101 gtggtgtttg gacttatggt tcaggtaacg gtggtgctta ctcaaactac tatcaccctt
     5161 caaaatacca tagttcaaca gttgttagca gaaaaactgg ttcatctgac aagggatatg
     5221 ctggtgctgg agggacttct cgtgcatgga ttcgtacttc ttggggagag aaagttgcat
     5281 tctactataa tgtttagaat ggtatagccc tattgtttag ctttacttgg tcaccttctc
     5341 actaagtgac caagtaactt tttggaggaa atgatgaaac gtttatttat tttgatttca
     5401 atggtattgg tatcgcttta tatggtgata acttccgttg accatcgaga agagatttta
     5461 tttggtaact atccttctgt tgatgtgaca ggaatgatga caaatcaacc agtagctagt
     5521 cgcgaagagg tgacagaggc tttgagtcac ttggcggtag agcacaatag tctcattgct
     5581 cgtcgaatcg ttgagccaaa tgaagctgga gaaacacgct ttacctatgc cacttatggt
     5641 gagggaaagt ttccagaagg tctgaccatt tcctccaagg agagtgcaga aacgagtgat
     5701 ttattagggt cttacttgat tgtatcagga agtttggatg gagtgagctt acagaccacc
     5761 ttgaaagagc ttggttatca aggctttgtt tcgaatggag aagatccatt ttcgatagtc
     5821 ttactattga cggccacccc tatggtgcta ctgagtttag ctatttttct gctgaccttt
     5881 atgagtctga ccctgattta tcggatcaaa tcccttcgtc aggcagggat tcgcttaata
     5941 gctggtgaga gcttgtttgg agttgctctc agaccagtgt tagaagatgt gagacagctt
     6001 atctgctcag tgctggtatc cagtcttttg ggattgggga ttctctggta tcaaggtgcc
     6061 ttgtttatgg caacggtgca actggtcatc attgctcttc tactttatgg attgaccttg
     6121 gcagggattt ctaccttact aagtgtcgtc tatctacttg gtttacagga aaatagtctg
     6181 gtggatctat tgaaagggaa actccctctc aaacgtatga tgacattgat gatggtgggg
     6241 caactcttag ctgtattggt ggtcggatcg agtgcgacag ctctcctacc ccactaccgt
     6301 gaaatgcagg aaatggagag agctagcaat aaatggagcc agtcctcaga ccgttaccgt
     6361 ctatcctttg gttggtctag tgcatttgcc gatgaagaag gaacgcgtaa ggataatcgt
     6421 gagtggcaga catttactga agaacggtta gccaatacag actcttttta tattatgagc
     6481 aatgttgaca atttctcaga tggagcagaa gtggacctag atggcaatcg tctcagtgac
     6541 tacacaccgt cagggaatgt tatctatgtc tcaccgcgct atctgataga agaaaagatt
     6601 accgtttctt cagagtttat ggacaagatg caaaacttgt ctgagggaga gtttgggctg
     6661 atcttgcctg agagcttgcg agagcagtct atctactacc aaggattgtt tacagattac
     6721 ctgcaaaact tttcatctga aagtgtagaa gtgacgagtc agaaacacta cctcccacag
     6781 gtaaggctag cttttacaga aataggacag gaacgtttcc tctataatga tgggtacaag
     6841 acaacacgcc agtacctaaa agatccgatt attgtagttc taacgccgca agcgactgga
     6901 acaagacctg ttgcagggat gttgtgggga actacggcta atagtgcctt gaaactagat
     6961 cgatatggag acagcatcac agctctaaaa gagcaaggtc tgtatcacaa ggtttcttac
     7021 ttggtaaaaa gccagctatt ttttgccaag gtactaaatg acaaacgggt ggagttttac
     7081 tctctcctta ttgggacgat tttgaccctg tctacggcta tcttgttatt tgattccatg
     7141 aatcttctct attttgagca gttcagacgg gatcttatga ttaaacgtct tgctggtatg
     7201 acaatctatg agcttcatgg caagtattta ctggcgcaag gaggagttct cttgcttggc
     7261 ctagtcctat ctagtatttt gacaagagat ggtttgatta gcgctctagt tgtagctttg
     7321 tttacgctta acaccctctt tattttagta aggcaggaca aaaaagaaga agctggtagc
     7381 atggcagtat tgaaaggaaa ataagatgat tgatattcaa ggattggaaa agaaatttaa
     7441 tgaccgcgcg attttctctg gtttgaatct caagctggag aagggcaagg tttatgcctt
     7501 aatcggaaag agtggaagcg gaaagacgac gctgctgaat atcttgggaa agctagaaaa
     7561 gatagatgga ggaagggttc tctatcaggg gaaagattta aaaaccattc ccactcgtga
     7621 gtattttcga gatcagatgg gctatctctt tcaaaatttt ggcctcttag aaaaccaatc
     7681 aatcaaagaa aatttggatt tgggttttgt tggtcagaaa atctcaaaag tagaacgttt
     7741 ggaaaggcaa gtgggggctt tagaaaaagt taatctaggg tatttggatt tagaacaaaa
     7801 aatttatact ttatctgggg gagaggccca acgagttgcc cttgcgaaaa ctattttgaa
     7861 aaatccaccc ttgattttgg cagatgaacc aacagcagct cttgatcctg aaaattcaga
     7921 ggaggttatg aatctcttgg tggatttgaa agatgaaaat cgaattatca tcattgcgac
     7981 ccataatccc ctagtctgga ataaggctga tgaaatcatt gatatgagga aacttgctca
     8041 tgtgtgaaaa aatccgtatt cgcagggtat ctgattatcc tagtgccaga ggtggtttat
     8101 aagatatcct catcatggaa aatatgacca atcatctcct tttggttcaa atccgagtgc
     8161 atggctattt gcttgatttt gctagtagtg aagggcaaag gcaaaagcat tatcgtttga
     8221 aaaatttacc tcaaacggtt gaactgacag tggatgatgt ggaggaggat gtggatttga
     8281 ccctacccga aaatcgaagt tatcaagaag ctgatttttt tgaacgcatg tttcgagaga
     8341 actgctaagg ccacttttaa agatttccaa gactatcttt cttcatgagg aaagatagtt
     8401 ttttggtatg attttcattc ccaaaataca aggggaatgt gttacaatag tagtaacaga
     8461 taatagaaaa gagaatagat gagaattgca gattatagcg tgaccaaggc agtgctggag
     8521 cgtcacggtt ttacctttaa aaagtccttt gggcaaaatt ttttgacgga taccaatatc
     8581 cttcaaaaaa ttgtggatac ggctgaaatt gatgatcagg tcaatgtcat cgaaatcggg
     8641 ccaggtattg gtgccttgac agaatttttg gctgagcgtg cagcccaagt catggctttt
     8701 gagattgacc accgtttggt gccaattttg gcagataccc tgcgtgattt tgataatgtg
     8761 accgtagtta acgaagatat tctcaaggtt gatttggcgc aacatatcca gaattttaaa
     8821 aatcctgacc tgccaatcaa ggtagtggct aatttgcctt actacatcac gacgcctatt
     8881 ctcatgcact tgattgagag tggcattcct ttttgtgagt ttgtggtcat gatgcagaaa
     8941 gaagtagcgg accgcatttc agcccagcct aacaccaagg cttacggtag cttgtctatc
     9001 gccgtgcagt attacatgac agccaaggtt gcctttatcg tgcctcgtac ggtctttgtg
     9061 ccagcgccaa atgtggattc agccatcttg aaaatggtgc gtcgtccaga gccagccgta
     9121 gcagtagaag atgagaactt tttctttaag gtttccaagg ctagttttac ccatcgccgc
     9181 aagaccttgt ggaataactt gacaggttac tttggtaaga ctgaagaggt caaggacaag
     9241 ctgaccaagg ctttggacca ggcaggcttg tcaccaagtg tgcgtgggga agctctcagc
     9301 ttggcagaat ttgccggtct agcagacgca cttaaagggc aaggactcta agatgcaggg
     9361 acaaatcatt aaagccttgg caggtttcta ctatgtggag agtgatggcc aggtttatca
     9421 aacacgcgcg cgtgggaatt tccgtaaaaa aggccatacc ccttatgttg gggactgggt
     9481 agatttctct gccgaggaaa attcagaagg ctatatcctc aaaattcacg aacggaaaaa
     9541 cagtctggtt cgtccgccta ttgtcaatat cgatcaagct gtagtaatca tgtccgtcaa
     9601 ggaacctgat tttaacagca atttgctgga tcgtttcttg gttcttttgg agcacaaggg
     9661 catccatccc attgtctata tttccaaaat ggatttgttg gaagataggg gagaactgga
     9721 tttttaccag cagacctatg gtgacatcgg ctatgacttt gtgaccagta aagaggaact
     9781 cctgtctttg ttaacaggca aggttacggt ctttatgggg cagacaggtg ttgggaagtc
     9841 aactcttctc aataaaatcg caccagacct caatcttgaa acgggagaaa tttcagacag
     9901 tctaggtcgc ggtcgccata ccactcgagc tgttagtttt tacaatctca acgggggtaa
     9961 aatcgcagat acaccaggat tttcatcctt ggactatgaa gtatcaaggg ctgaagacct
    10021 caatcaggct ttcccagaga ttgctactgt tagccgagat tgtaagttcc gtacttgtac
    10081 ccatacccat gagccgtctt gtgccgtcaa accagctgtt gaagagggtg ttattgcaac
    10141 cttccgtttt gacaattacc tgcaattcct tagtgaaatt gaaaatcgta gagaaaccta
    10201 taaaaaagtc agcaaaaaaa ttccaaaata aggagaaacc tatgtctcaa tacaagattg
    10261 ctccgtcaat tctggcagca gattatgcca actttga
//
