LOCUS       NZ_FEEQ01000018        10867 bp    DNA     linear   CON 04-JAN-2025
DEFINITION  Streptococcus pneumoniae strain 2842STDY5644069, whole genome
            shotgun sequence.
ACCESSION   NZ_FEEQ01000018
VERSION     NZ_FEEQ01000018.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2016) SC, Wellcome Trust Sanger Institute, CB10
            1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900047005.1-RS_2025_01_04
            Annotation Date                   :: 01/04/2025 01:43:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,253
            CDSs (total)                      :: 2,202
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 86 of 184
            Pseudo Genes (incomplete)         :: 88 of 184
            Pseudo Genes (internal stop)      :: 60 of 184
            Pseudo Genes (multiple problems)  :: 40 of 184
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:55:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 17013
            Orig. end                         :: 27880
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FEEQ01000018.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10867
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10867
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10867
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5867](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5867
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(1012..1167)
                     /gene="blpC"
                     /locus_tag="A1T31_RS10065"
                     /old_locus_tag="ERS367508_01975"
     CDS             complement(1012..1167)
                     /codon_start=1
                     /gene="blpC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) L_biotic_A"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358809.1"
                     /locus_tag="A1T31_RS10065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS367508_01975"
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="WP_000358817.1"
                     /sec_met_domain="L_biotic_A (E-value: 7.3e-07, bitscore:
                     20.4, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKKQNLTSFQELTTTELNQITGGGWWEELLHETILSKFKITKAL
                     ELPIQL"
     gene            complement(1224..2585)
                     /locus_tag="A1T31_RS10070"
                     /old_locus_tag="ERS367508_01976"
     CDS             complement(1224..2585)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /locus_tag="A1T31_RS10070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS367508_01976"
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="WP_001069082.1"
                     /transl_table=11
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKEI
                     TVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTALET
                     QLQRLEKQKEGLGILKQSLEKATDLFSSEDEFGYHNTFMNFTKQSHDIELGISKTNTEV
                     SNQANLTNSSSSAIEQEITKVQQQIGEYQELRDAIVNKRARLPTGNPHQSILNRYLIAS
                     QEQTQGTAEEPFLSQINQSIAGLESSIASLKLQQAGIGSVATYDNSLATKIEVLRTQFL
                     QTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGKNRIPTGTEI
                     AQIFPIITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQTIDQTPT
                     RTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTAKKTYFDYFKDKILTHSD"
     gene            complement(2596..4721)
                     /gene="blpA"
                     /locus_tag="A1T31_RS10075"
                     /old_locus_tag="ERS367508_01977"
                     /pseudo=""
     CDS             complement(2596..4721)
                     /codon_start=1
                     /gene="blpA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /locus_tag="A1T31_RS10075"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ERS367508_01977"
                     /product="peptide cleavage/export ABC transporter BlpA"
                     /pseudo=""
                     /sec_met_domain="Peptidase_C39 (E-value: 2.5e-44, bitscore:
                     141.6, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKTN
                     KEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQTK
                     KDYLIIGDPDPSVKITKMSKERFFSEWTGVAIFLAPKPSYQPHKDKKNGLLSFLPLIFK
                     QKSLIAYIVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVITYILQQVM
                     SFSRD"
     misc_feature    complement(4841..4854)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 26.06"
                     /tool="antismash"
     gene            5001..5255
                     /gene="blpM"
                     /locus_tag="A1T31_RS10080"
                     /old_locus_tag="ERS367508_01979"
     CDS             5001..5255
                     /codon_start=1
                     /gene="blpM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /locus_tag="A1T31_RS10080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS367508_01979"
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="WP_001093257.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 1e-20, bitscore:
                     65.2, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTKMMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGLG
                     TAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            5271..5474
                     /gene="blpN"
                     /locus_tag="A1T31_RS10085"
                     /old_locus_tag="ERS367508_01980"
     CDS             5271..5474
                     /codon_start=1
                     /gene="blpN"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /locus_tag="A1T31_RS10085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS367508_01980"
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="WP_001099490.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 4.4e-14,
                     bitscore: 44.0, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGKILGPW
                     GAAIGGIGGAVVCGYLAYTATS"
     misc_feature    complement(5695..5710)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 18.42"
                     /tool="antismash"
     gene            5718..5867
                     /gene="blpO"
                     /locus_tag="A1T31_RS10090"
     CDS             5718..5867
                     /codon_start=1
                     /gene="blpO"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /locus_tag="A1T31_RS10090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="WP_001809846.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 6.4e-12,
                     bitscore: 37.1, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDGC
                     ATTV"
     gene            5971..6090
                     /locus_tag="A1T31_RS10095"
     CDS             5971..6090
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /locus_tag="A1T31_RS10095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="WP_000346296.1"
                     /transl_table=11
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     misc_feature    complement(6226..6239)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.08"
                     /tool="antismash"
     gene            6571..6929
                     /locus_tag="A1T31_RS10100"
                     /old_locus_tag="ERS367508_01982"
                     /pseudo=""
     CDS             6571..6929
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /locus_tag="A1T31_RS10100"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ERS367508_01982"
                     /product="immunity protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MIFFVRLFLGQLPLLVSTYLFLFRQFSNFF"
     gene            7558..7941
                     /locus_tag="A1T31_RS10110"
                     /old_locus_tag="ERS367508_01983"
     CDS             7558..7941
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /locus_tag="A1T31_RS10110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS367508_01983"
                     /product="hypothetical protein"
                     /protein_id="WP_000877381.1"
                     /transl_table=11
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGVF
                     LLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHIFI
                     VFNALMYLLSSSQVSLKKNKLSS"
     gene            7993..8682
                     /locus_tag="A1T31_RS10115"
                     /old_locus_tag="ERS367508_01984"
     CDS             7993..8682
                     /EC_number="3.4.-.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /locus_tag="A1T31_RS10115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS367508_01984"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_000760532.1"
                     /sec_met_domain="Abi (E-value: 3.4e-14, bitscore: 44.4,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFVW
                     IQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKHVQ
                     STAEGWAVLIGYSGTNFAELGIYITLFFLTPLMEELIYRGLLQHAFFKHSRFGLDLLLP
                     SILFALPHFSSLPSLLDIFIFATSGIIFASLTRYTKSIYPSYAVHVINNIFATLPFLLT
                     FLHRVLG"
     gene            8724..8957
                     /gene="blpZ"
                     /locus_tag="A1T31_RS10120"
                     /old_locus_tag="ERS367508_01985"
     CDS             8724..8957
                     /codon_start=1
                     /gene="blpZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /locus_tag="A1T31_RS10120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS367508_01985"
                     /product="immunity protein BlpZ"
                     /protein_id="WP_000276498.1"
                     /transl_table=11
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFNF
                     LNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            9108..9719
                     /locus_tag="A1T31_RS10125"
                     /old_locus_tag="ERS367508_01986"
     CDS             9108..9719
                     /EC_number="3.4.-.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /locus_tag="A1T31_RS10125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS367508_01986"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_000394048.1"
                     /sec_met_domain="Abi (E-value: 2.3e-21, bitscore: 67.4,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGVFFQNESALILVSLL
                     VTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHLAE
                     VSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSNVPS
                     FIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            9880..10674
                     /gene="ccrZ"
                     /locus_tag="A1T31_RS10130"
                     /old_locus_tag="ERS367508_01987"
     CDS             9880..10674
                     /EC_number="2.7.1.15"
                     /EC_number="2.7.1.229"
                     /GO_function="GO:0004747 - ribokinase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /GO_function="GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /GO_process="GO:0051726 - regulation of cell cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ccrZ"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /locus_tag="A1T31_RS10130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS367508_01987"
                     /product="cell cycle regulator CcrZ"
                     /protein_id="WP_000363002.1"
                     /sec_met_domain="APH (E-value: 7.6e-20, bitscore: 63.1,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGLA
                     REQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQLS
                     RLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDVRHS
                     NWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTVLSKL
                     YWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     misc_feature    complement(10837..10851)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: weak, score: 17.11"
                     /tool="antismash"
ORIGIN
        1 tttattgatt atgatattcg tttgcttcat agttttagtc tttttgaaat ggttagacta
       61 tgatttcact agattgagaa gggagtttct agatacaggt tttcaaaagt ctcttactaa
      121 gattaactgg gcaatggggg cttattatct agtgatgcaa agtctatctt accttgaata
      181 tgaacaaggt attcaatcaa cgactgttcg ccatctcatc ctagtgtttt acctactctt
      241 ttttatgggg ggtatcaaga aattggatac ctatttgaag gaaaaacttc aggaggaact
      301 gaaccaagag cagaccttgc gctacagaga tatggaacgc tatagtcggc atatagagga
      361 actttacaag gaaattcgga gttttcgcca tgactacact aacctcttaa ccagcttacg
      421 tttgggcatt gaagaggagg atatggagca gataaaagag atctacgact cggtcttaag
      481 ggattccagt cagaaattgc aggacaataa atatgacctg ggcagattgg tgaatattcg
      541 tgaccgtgcc ctcaagagtc tcctagctgg aaaatttata aaagctagag aaaagaacat
      601 tgtctttaat gttgaagttc ctgaggagat tcaggttgag gggatgagcc tacttgattt
      661 tctaaccatt gtgtctatcc tttgtgacaa tgctattgaa gctagtgcag aggccagtca
      721 acctcatgtt tcaatcgcct ttttaaaaaa tggagcacag gagactttta tcattgaaaa
      781 ctccatcaaa gaagagggca tcgatatttc tgaaatcttc tcctttggag caagttctaa
      841 aggggaggag agaggagttg gtctctatac cgttatgaaa attgtggaaa gtcatcccaa
      901 taccaatcta aatactacct gccaaaatca agtctttcgt caggtactta ctgtgataca
      961 tgcagaatga taaaaaacaa gaccgagagt tcttgtttct cggtcttgtt tttatagctg
     1021 aataggtagt tcaagtgctt ttgtgatttt aaatttactt aaaattgttt catgtaagag
     1081 ttcttcccac catcctccac ctgtaatttg gttgagttcg gtagttgtta gttcttgaaa
     1141 tgaagttagg ttttgtttct tatccatgtt atgattctcc tttttgataa gataataaat
     1201 agttatagag tgttatctga aaattaatca gaatgggtta aaattttatc tttgaaataa
     1261 tcaaaatatg ttttctttgc agttacacta gtgacgcgac cttgtaagcc atattggatg
     1321 agtttactat cctcattaga tagttttgca agagcggtta atttaaagag attaccttgc
     1381 tctgttctgg taggagtttg atcaattgtc tgaagttggc cgatgatggt ggtgccgtga
     1441 tttccaatct tctccagttt taatcttaca gtttgtcctt tatctagtag aggtagatag
     1501 tcagaagata cgtagtaagt gattagtact tctcttgtat ctgtgatgat agggaatatt
     1561 tgagcaattt ctgtaccagt tggaattcta tttttacctt caaattcgct gttcagatga
     1621 acgatacctt tacttggagc ggttaaggta ttgttttcta agcgctgtgt cgcttgatct
     1681 agttgtactt ttaattctgt taattgattc tccacggtta gttgttgctg tgaggctgtt
     1741 tgtaaaaact gagtgcggag tacttcaatt ttggttgcta aactattatc ataagttgct
     1801 acacttccga tgccagcttg ctgaagtttg aggcttgcga tagatgattc cagacctgca
     1861 atactttgat taatttgaga taaaaatggc tcctctgccg ttccttgtgt ttgttcttga
     1921 gaggctataa gataacgatt taaaattgac tggtgcggat tgccagttgg taagcgagct
     1981 cttttattta cgatagcatc tctcagctct tgatattctc caatttgttg ttgaactttt
     2041 gtaatttctt gttcgatggc tgatgaactg ctattggtaa gattagcttg atttgaaact
     2101 tcagtgttag tctttgagat acccagttca atatcatggg attgtttagt aaaattcata
     2161 aaagtattat ggtagccaaa ctcatcctcg ctagaaaaaa gatcagtcgc tttttctaag
     2221 ctttgtttca agattccaag tccttctttt tgcttctcaa gcctttgtaa ttgagtttct
     2281 aaggcagttt tctgactctc ttccattgtt tcagagtatt tgatgagtaa gtcacctttc
     2341 tcaactactt gatttgccac taaatgatta gcaaggatag gattatcact ggtcgactga
     2401 atagatgcaa tgacacttgt aggggcgatt tctccttggg aagtaacagt aatttctttt
     2461 gtggcaacaa gggagaaaat caagatgaaa gtaaacagta atgaaagagg tataattaac
     2521 actgtcgcat agttatggta acgtctctga taaaactcga cgcttctaaa aagattagga
     2581 ttcatgacat tctccttatt tattgaatag atgatggtag aagccttgcg cctgcattaa
     2641 ctcttggtga ctaccaactt caatgatttt cccctggtca agaacaatga cacggttagt
     2701 tcgttcggct atactgagac gatgggctac aaagagaatg gttttatcag ttagggacat
     2761 aagattatct ataacctttt tctcagtcaa gacatcaaga ccgctagtag cttcatctag
     2821 tattaaaaca ggagatttag ttaaaagagc acgagcgagg gcgattcgtt gcttctgtcc
     2881 tcctgataga ccagctccat cagagagctg agtttgatag cccataggca ttctttcaat
     2941 gtcttgacgg atttcagcta cttcacaagc ttttagaata tcttcttgac taatcatatg
     3001 attaccgccc aaggttaagt tttccaaaat agagccatta aagatatagg cttgttgggg
     3061 taggtaatta atatgacggc gcaagacttt tttatcaatg tttttaatat cctgatgatt
     3121 gatggaaata tgccctttgt agggttcaaa gaaattgaca atcattttgg ctaaagttgt
     3181 tttaccagaa ccactaactc caactaggct agccttatct ccttgtttaa tcgtgagatt
     3241 aatatctgtt aaggtatctc gtccaaaacc atacttataa gaaaggtcat caaattcaat
     3301 atcgcccatc aaaaaatgtg aatgaacagg gttttcttga acttgaaatt cagattcgac
     3361 tagatagact tcgttcaaac ggttattagc gatcttcgca gattggagtt tggtttggag
     3421 gttgataata ttttccatag gagttgtaaa gtaagaaaaa agtgtgttaa aggtaatcag
     3481 ctgaccgata gaaattttac ttgacatgac taattgagcg ccaaaccata ggataaggat
     3541 attcagaact aattttgttc cctgctttaa actcgtttgt aaaatagaat atttactgag
     3601 cttaaaggat ttttccaaat aatctacaaa ttcgctgtct atattttgat agcgattttc
     3661 ttcactcgtg agcgacttta tagtttcaat cccgttgata tctttgataa tggcagagct
     3721 aaccatagaa ttactttgca tgacatcatg gttcattttt tcgaaaggtt tcataaaaga
     3781 aaagatgatg aacatgtata taggaatgga aataagagaa agaaggaaga gattagggtt
     3841 ttgtgccagc aagactcccc ctacaagaat cagaatagaa acatccagaa aaagagaaag
     3901 aatggtagaa gccaaggcat ctataataga gttagcatct gtgaaccgtg aaatgatttc
     3961 tcctgtacga cgtgtcgcaa agaaagacat gggaagttca aaaatatggc gaatatagga
     4021 taaaatcaca tcaatgctta atctctggag aagctcatga cttgttggag gatataggtg
     4081 ataaccagac caactgagat gattcctaaa gttgatttca tctgatttgg gatgtattca
     4141 tctaagattc cttggagata ataagaacca ccgatattga taatagtgac caataagctt
     4201 gagagaacaa tgtaagcaat gagagatttt tgtttgaaaa tcagaggaag gaagcttagt
     4261 agaccattct ttttatcttt atggggttga tagctgggtt tgggagctag aaaaatagct
     4321 actccagtcc attcagagaa aaagcgttct tttgacattt tagtgatttt tacagaaggg
     4381 tcaggatcac caataatcag atagtctttc tttgtttgat agacaacata gtaatgttgg
     4441 agttttcctt ctttgttaac gtgaacgata aatggatagg ggacatcact catgtcaaag
     4501 agcgttttat ctgcttgaac aggtcttgtt tcaaagccca tttcatcagc ggcttttaca
     4561 atgccaagag cagtcgtccc ttctttattg gtctttgcaa gttctctcaa gtgagctaga
     4621 gaaaaatctg aaccatagaa tttagcaatc gaggctaagg cagcaacacc gcagtctctc
     4681 gcatctattt gaggaacaaa tgtacgttta taagaagtca ttggcaattc ctttcatata
     4741 gtagataggt atattatgcc ataaaaataa gttcatattt tattaaatct tgaatagtca
     4801 ttgaaacttc ctgaatggta aaaaataatt agaaattatc tttttttaaa aaatttagag
     4861 gtggtttcaa atgaaaagct aattcaagac gtttcgatga ctattcaaga tctggatgaa
     4921 aaattttaaa aaacgatgat atactaaact tgtcaaagtt acaacaagac aaaaattaaa
     4981 aataaaaaag agtatttgtc atgaatacaa aaatgatgga acaatttcat gaaatggata
     5041 taactatgtt atctagtatt gaaggaggca agaataattg gcaaactaat gtcttagaag
     5101 gtggtggtgc tgcttttggt ggctgggggc tagggacagc tatttgtgct gcgagtggtg
     5161 ttggagcacc atttatggga gcatgtggat acataggagc taaatttggt gtggatcttt
     5221 gggcaggagt aacaggggca acgggtggat tttaataaaa ggagacttgt atgaatactt
     5281 attgtaatat aaatgaaaca atgctgagtg aggtttatgg aggtaattcc ggaggagcag
     5341 ctgtagttgc tgctttaggt tgtgcagcgg gtggagtgaa atatgggaaa attctaggac
     5401 catggggcgc tgcaatagga ggaattggag gagcagtggt ttgtggatat ttagcctata
     5461 ccgctacatc ataaaaatga aagctaaatt ttatgaaggc tatattcttt attattctct
     5521 tcgcttttca aacctatctt atttatctat ccataagtat ctctgataaa aaacaaaaga
     5581 ctattgaatt aacatcattg aattgttttg tcatattatt tctaatctat gataaattaa
     5641 tttttctctt tattgcctat gtttttttga taatttttat attaaacttg ttccgtaact
     5701 gaaaagtgac atactatatg aatacaaaaa tgatgtcaca attttctgtt atggataatg
     5761 aaatgcttgc ttgcgttgaa ggtggagata ttgattgggg aagaaaaatt agttgtgcag
     5821 caggggttgc atatggcgca attgatgggt gtgcaacaac ggtttgacat ttctattggg
     5881 accatttgct ataggaatag gtgtaactgg tgctgcaggt ggagctatac ttggaggtgt
     5941 agcctatgca gcaacatgtt ggtggtaatt atggatttta aaagttttat tattggttta
     6001 gtagttggta tatttggtcc ttatatggat gacttaatta gaaaaatatt ttcaaaatct
     6061 tccaaaaagg atacggatag tactctctaa aaaataaatt tctcccctgt atttgctagc
     6121 ttaatttaca ggggagtttc tttaatattg tttcgcctgt caaaatgata tgtgatagca
     6181 ggatgaaaag tctacaaaca agttggtatc aatacaatat aaagaggaaa gtatgttttt
     6241 catgcttgct tttttaattt ttacaattca agaggttttg atgaccattt atgatttgag
     6301 tgatccaagg tcaaaataag tgctatacta gcagtgtaaa tgttcttgct caactaagat
     6361 aaatcgcaca tttaggagga aaacttatga agaaaaaaat actgattatt ttcgttctgt
     6421 atctgatcat gtccatcttt ctttatccgc ttagggagag tatttggtat aatctatttt
     6481 ataccatagc ctatatgatt gcggttatga tctatttttc tttaattaaa aagaaagaaa
     6541 agaaatgaaa gattttcttg attaaaaaga atcatattct ttgtgagatt gttcctgggt
     6601 cagttgcctt tgcttgtttc tacttatcta tttctatttc gtcagttttc caatttcttt
     6661 tagtagttat taacttgact tctattttgg ttactgttta tcttactagg gaaatgagat
     6721 taagagagtt tgaagatgat gatttggtta gtcctagaac caatcaactc atgtttatcg
     6781 gcttgacagg ctttatgtct attatttgtt tgtatagagg tatcacagca ggagaatctt
     6841 atcaacaact aatcgcttat attggtgcta ttctttgctt gatcatcatg cttctactca
     6901 tttggggttt gaagtattat aaaaagtagg ggctaatgaa gtttttgcaa tttaggaggt
     6961 ttcgatgaca attcaagatt tggatgaaaa attttaaaaa acaatgatat actaaacttg
     7021 tcaaagttgc aacgggtgct gctacattat gttaaaaatc gctcttgatt gataagttgg
     7081 atttgttagg aaaggagtaa ataattatgg ataagaaaaa gatagtttcg actataatat
     7141 gtatagtatt tcttgtagtg tcagtagata atttttttag agacttaacc ccgttactgt
     7201 tcattttaaa tattattggc ctgtcttgct tttcagtgct aacatacatt aacataaaag
     7261 agatactctt aaatataagt aaatgatgat ttggttagtc ctagaaccaa tcaacttatg
     7321 tttatcggct tggcagattt tatgtttgtc atttgtttgt atagaggtat ttcagaaaca
     7381 gaattctatc aacaactaat cgcttatata ggtgtattct ctgcttgttt atcacgcttc
     7441 tgctcatgtg gggcttgaag tattataaaa agtagctttc tatccttttc tgacaggata
     7501 tttgagtaga aggctctatt aaattgttag tgaaataaat gaatggaggt atttaatatg
     7561 aaatacagat tattttttgt tattttcttg agtagtgtgt tggatattct tttagggaca
     7621 tttttacaaa tctctatcgt atctattggg tggcttgttc tttacagtgg tttgtttgaa
     7681 gcaggagttt tccttcttgc taataaaggg gtggcggtaa aaatcaagga agtagatatt
     7741 cgaaatcgct ttaaatttat ttttggaaaa accttatggt ttcaaattct tttgctcatc
     7801 tttttgataa tcaaacttta tcttggtttg gatgcgaggt tgattttatt ctatggacat
     7861 attttcattg tctttaatgc cttaatgtat ctattatcta gtagtcaggt tagccttaaa
     7921 aaaaacaaac tgtcttctta atcttacagc agtagagcct gacaaggtga ggtcatagta
     7981 gggaggattt ctatgaaaaa gtatcaactt ctattcaaaa taagtgcagt tttctcttac
     8041 ttatttttcg tatttggtct ttctcagatg acgcttatta ttcaaaatta ttggcaattt
     8101 tcttcccaga ttggcaattt cgtctggatt caaaatttct tgagtttgct atttagcgga
     8161 gtcatgattt ggattctggt taagacaggt catggttatc tctttcacat tccaagaaaa
     8221 aaatggcttt ggtattcgat tttgacagta ttagtggtag tgctccagat ctcttttaac
     8281 gttcagacag ctaaacatgt tcagtcaact gctgaaggtt gggctgtatt gatcggttat
     8341 agtgggacca actttgctga gctaggtatc tatataactt tgttctttct gactccactt
     8401 atggaagagc taatctatag aggattactg caacacgcct tttttaagca ttcgagattt
     8461 ggccttgatt tgcttcttcc gtcaatttta tttgctcttc ctcatttttc aagcctgcct
     8521 agtttgttag atatcttcat ctttgcaaca tctggcatca tctttgctag tttgacccgc
     8581 tataccaaga gcatttatcc ttcctatgcg gtgcatgtga tcaataatat tttcgcaaca
     8641 ttaccatttt tgctgacttt tttacatagg gtcttggggt aaaaaaacaa aaggcttgct
     8701 tttcagccat agaggagatt atcatgtata aacacttatt tttcctagat tccaaaactt
     8761 tagatcggtt gacaccctat attctagtct tggcttctga caccattgct tttaatgttt
     8821 ttgtgctaac ctttgtatct gcggtggttt ttaatttcct aaattccatg ctagctttaa
     8881 tggctatatt cataggggct ggctatgtgg tcggattttg gttactaata ctcaatgaaa
     8941 atcaaagagc aaactaggaa actagccgca ggctgtactt gagtacggca aggcgacgtt
     9001 gacgtggttt gaatttgatt ttcgaagagt ataaaatggt ttgttttgga aaggctagaa
     9061 ctaaagaatg acgcgtagga agtctgtttg gtgaggagga tagttttatg gagttttttg
     9121 ataaatttca tgccttgtgt tttggatttt tagtactaat aattgtcatt acagttcctt
     9181 atacgattaa ccatggggtt ttttttcaaa atgaatctgc attgattctt gtaagtcttc
     9241 ttgtaacctc gctgagtgtt gcttatgcta gaaagtttga aatgatttct tttgggatgt
     9301 taagcaagaa acaacttttg cttttcattg caatctttct tctaagtgta cttgagacgc
     9361 tggtttatat tcatttcttc gctgtttctt ctggctcagg ggtccaacac ttggcggaag
     9421 tcagcagagg aatttccctg tctttgattt tgactacctc agtttttggc cccatccagg
     9481 aggaactcat tttcagagga cttcttcaag gtgcggtttt tgacaattct tggttagggc
     9541 ttgtgctaac ttcctctctc ttttctttca tgcatggacc ttctaatgtc ccttcgttta
     9601 ttttttatct acttgggggt ttgttgctgg gctttgctta taaaaagagt caaaacctat
     9661 gggtttctac tctagtccac atgttttaca acagttggcc actcctatat tatttataaa
     9721 aattatgaaa aggtaagcag aagatggtgc ttaccttttt caagctataa tgatacccaa
     9781 gccaatccag aggcttttct ttgagaatcg ggttagagtg gttgacgaat aggccaaaaa
     9841 ctagtagaat agtaaggaaa ctttatacgg aggaaagaaa tggatttggg tgataatgag
     9901 ctaacactga ctcccatacc tgggaaaagt ggcaaggctt atatgggtag ctatcctgac
     9961 gggaagcgca tctttgtaaa aatgaacacc tctccaatcc tacctggtct agctagagaa
    10021 caaattgctc cacaattatt atggagtcgt cgtttggcag atgggcgtga tatgtgtgct
    10081 caagaatggt tgacaggcaa gatattgacc ccctatgata tgaatcgtaa gcaaatcgtc
    10141 aatattttaa cccgtcttca tcgctcacgt ccgttgatga cacaattgag tcgtttgggc
    10201 tatgccatgg aaacacctgt agatttacta cagtcttggc aggaaacggc tccagatgct
    10261 ttgcgtaaaa atcattttat cagtgaagtg atggctgatt tacgtcagac tattccagga
    10321 tttagagagg accatgcgac cattgtccat ggagatgtac gacatagtaa ttggattgag
    10381 acagatagtg gcttgattta tttagtagat tgggattcgg ttcgcttgac cgatcgcatg
    10441 tttgatgtgg cccatatgct ctgccattat atttcagaac atcagtggaa ggaatggttg
    10501 acctactacg gttacaagta caatcaaacg gtattaagta aattgtattg gtatggtcaa
    10561 ttgtcttatt tgagtcagat ttccaagtat tatatgaacc aagatttaga aaatgtcaat
    10621 cgggagattc atggtttgcg tcatttccga gacaagtatg gaaagagaag atgagagtta
    10681 gaaatcgtaa aggggcaaca gaattactag aggcaaatcc ccagtatgtg gtcctcaatc
    10741 ccttggaagc caaggcaaaa tggcgggact tgtttggcaa tgataatccc attcatgtgg
    10801 aagttggaag tggaaagggt gcctttgttt caggtatggc caagcaaaac cctgacatca
    10861 actatat
//
