LOCUS       NZ_FDFR01000008        10297 bp    DNA     linear   CON 22-DEC-2024
DEFINITION  Streptococcus pneumoniae strain 2842STDY5753301, whole genome
            shotgun sequence.
ACCESSION   NZ_FDFR01000008
VERSION     NZ_FDFR01000008.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2016) SC, Wellcome Trust Sanger Institute, CB10
            1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900022985.1-RS_2024_12_22
            Annotation Date                   :: 12/22/2024 02:12:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,086
            CDSs (with protein)               :: 2,086
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 71 of 173
            Pseudo Genes (incomplete)         :: 93 of 173
            Pseudo Genes (internal stop)      :: 58 of 173
            Pseudo Genes (multiple problems)  :: 41 of 173
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:29:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 67825
            Orig. end                         :: 78122
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FDFR01000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10297
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10297
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10297
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5297](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5297)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(842..1714)
                     /gene="rsmA"
                     /locus_tag="A1O50_RS09485"
                     /old_locus_tag="ERS409084_01855"
     CDS             complement(842..1714)
                     /EC_number="2.1.1.182"
                     /GO_function="GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="rsmA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /locus_tag="A1O50_RS09485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS409084_01855"
                     /product="16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="WP_001216869.1"
                     /transl_table=11
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEID
                     DQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDILKV
                     DLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRISAQ
                     PNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVEDENFF
                     FKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLAEFAGL
                     ADALKGQGL"
     gene            complement(1965..2258)
                     /locus_tag="A1O50_RS13620"
                     /pseudo=""
     CDS             complement(1965..2258)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333255.1"
                     /locus_tag="A1O50_RS13620"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYLL
                     DFASIEG"
     gene            complement(2251..2892)
                     /locus_tag="A1O50_RS09490"
                     /old_locus_tag="ERS409084_01857"
     CDS             complement(2251..2892)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /locus_tag="A1O50_RS09490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS409084_01857"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000565530.1"
                     /transl_table=11
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLLN
                     ILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFVGQ
                     KISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILADEPT
                     AALDPENSEEVMNLLVDLKDENRIIIIATHNHLVWNKADEIIDMRKLAHV"
     gene            complement(2894..4924)
                     /locus_tag="A1O50_RS09495"
                     /old_locus_tag="ERS409084_01858"
     CDS             complement(2894..4924)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /locus_tag="A1O50_RS09495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS409084_01858"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_050217366.1"
                     /transl_table=11
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMTNQ
                     PVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGELPEGLTISSKES
                     AETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLLSLA
                     IFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGIALRPVLEDVRQLICSVLVSSLLGLG
                     ILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGKLPLKR
                     MMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGWSSAFAD
                     EEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTPSGNVIYV
                     SPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYLQNFSSESV
                     EVTSQKHYLPQVRLAFTEIGQERFLYNDGYKTTRQYLKDPIIVVLTPQATGTRPVAGML
                     WGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRVEFYSLLIGTI
                     LTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGGVLLLGLVLSSI
                     LTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(5001..5297)
                     /locus_tag="A1O50_RS09500"
                     /old_locus_tag="ERS409084_01859"
     CDS             complement(5001..5297)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /locus_tag="A1O50_RS09500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS409084_01859"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000815394.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 2.8e-21,
                     bitscore: 68.6, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQTVKKLALVASIAATLGGSVAVASAAVQYPEGGVWTYGSGNGG
                     AYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     misc_feature    complement(5447..5460)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 22.05"
                     /tool="antismash"
     misc_feature    complement(5537..5546)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     gene            complement(5617..6516)
                     /locus_tag="A1O50_RS09505"
                     /old_locus_tag="ERS409084_01860"
     CDS             complement(5617..6516)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /locus_tag="A1O50_RS09505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS409084_01860"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_061751196.1"
                     /transl_table=11
                     /translation="MLIGQKIKEIRIEKGVSRPYFCGDEQELTVRQLSRIESGASQPSL
                     PKLAYIARRLGVPVYSLMPNFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEIYK
                     TYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLLIRL
                     FFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVGVTDC
                     FNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAQLIGDE
                     FLVKKLTEEWQEDVKKYL"
     gene            complement(6588..7148)
                     /gene="rnmV"
                     /locus_tag="A1O50_RS09510"
                     /old_locus_tag="ERS409084_01861"
     CDS             complement(6588..7148)
                     /EC_number="3.1.26.8"
                     /GO_function="GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="rnmV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /locus_tag="A1O50_RS09510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS409084_01861"
                     /product="ribonuclease M5"
                     /protein_id="WP_000660005.1"
                     /transl_table=11
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINAQDIERIQ
                     RLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVRHAFLKRDEAVPKSKTKGRSLGIEHAS
                     YEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSNGKQ
                     LLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(7148..7921)
                     /locus_tag="A1O50_RS09515"
                     /old_locus_tag="ERS409084_01862"
     CDS             complement(7148..7921)
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /locus_tag="A1O50_RS09515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS409084_01862"
                     /product="TatD family hydrolase"
                     /protein_id="WP_000575617.1"
                     /transl_table=11
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIER
                     ALELVDEYDQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTAPK
                     EVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLEWAE
                     KFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKILVETDAPYLAPVPKRGRENKTAYT
                     RYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(8226..>9197)
                     /locus_tag="A1O50_RS09520"
                     /old_locus_tag="ERS409084_01863"
                     /pseudo=""
     CDS             complement(8226..>9197)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_367670414.1"
                     /locus_tag="A1O50_RS09520"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ERS409084_01863"
                     /product="DUF1542 domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="KVEVAGDGTATIVFKDGSAITIPGNQLVAQDPKAQDSTKPTAEKS
                     TVKAPAQRVDVKDITHLTDEEKVKVAILQANGSALDGATINVAGDGTATITFPDGSVVT
                     ILGKDTVQQSAKGESVTQEATPEYKLENTPGGDKGGNTGSSDANANAGGGSQAGGSAHT
                     GSQNSAQSQASKQLATEKESAKNAIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQA
                     ALKEIENAKTMEDVKEAETIGVQAIVMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTY
                     QSPAGKQLPNTGSASSAALASLGLVVATSGFALLGRKTRRRK"
     gene            complement(9403..9537)
                     /gene="rpmH"
                     /locus_tag="A1O50_RS09525"
                     /old_locus_tag="ERS409084_01864"
     CDS             complement(9403..9537)
                     /GO_component="GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA]"
                     /GO_component="GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA]"
                     /GO_function="GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rpmH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /locus_tag="A1O50_RS09525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS409084_01864"
                     /product="50S ribosomal protein L34"
                     /protein_id="WP_000831905.1"
                     /transl_table=11
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA"
ORIGIN
        1 tctacgattt tcaatttcac taaggaattg caggtaattg tcaaaacgga aggttgcaat
       61 aacaccctct tcaacagctg gtttgacggc acaagacggc tcatgggtat gggtacaagt
      121 acggaactta caatctcggc taacagtagc aatctctggg aaagcctgat tgaggtcttc
      181 agcccttgat acttcatagt ccaaggatga aaatcctggt gtatctgcga ttttaccccc
      241 gttgagattg taaaaactaa cagctcgagt ggtatggcga ccgcgaccta gactgtctga
      301 aatttctccc gtttcaagat tgaggtctgg tgcgatttta ttgagaagag ttgacttccc
      361 aacacctgtc tgccccataa agaccgtaac cttgcctgtt aacaaagaca ggagttcctc
      421 tttactggtc acaaagtcat agccgatgtc accataggtc tgctggtaaa aatccagttc
      481 tcccctatct tccaacaaat ccattttgga aatatagaca atgggatgga tgcccttgtg
      541 ctccaaaaga accaagaaac gatccagcaa attgctgtta aaatcaggtt ccttgacgga
      601 catgattact acagcttgat cgatattgac aataggcgga cgaaccagac tgtttttccg
      661 ttcgtgaatt ttgaggatat agccttctga attttcctcg gcagagaaat ctacccagtc
      721 cccaacataa ggggtatggc cttttttacg gaaattccca cgcgcgcgtg tttgataaac
      781 ctggccatca ctctccacat agtagaaacc tgccaaggct ttaatgattt gtccctgcat
      841 cttagagtcc ttgcccttta agtgcgtctg ctagaccggc aaattctgcc aagctgagag
      901 cttccccacg cacacttggt gacaagcctg cctggtccaa agccttggtc agcttgtcct
      961 tgacctcttc agtcttacca aagtaacctg tcaagttatt ccacaaggtc ttgcggcgat
     1021 gggtaaaact agccttggaa accttaaaga aaaagttctc atcttctact gctacggctg
     1081 gctctggacg acgcaccatt ttcaagatgg ctgaatccac atttggcgct ggcacaaaga
     1141 ccgtacgagg cacgataaag gcaaccttgg ctgtcatgta atactgcacg gcgatagaca
     1201 agctaccgta agccttggtg ttaggctggg ctgaaatgcg gtccgctact tctttctgca
     1261 tcatgaccac aaactcacta aaaggaatgc cactctcaat caagtgcatg agaataggcg
     1321 tcgtgatgta gtaaggcaaa ttagccacta ccttgattgg caggttagga tttttaaaat
     1381 tttggatatg ttgcgccaaa tcaaccttga gaatatcttc gttaactacg gtcacattat
     1441 caaaatcacg cagggtatct gccaaaattg gcaccaaacg gtggtcaatc tcaaaagcca
     1501 tgacttgggc tgcacgctca gccaaaaatt ctgtcaaggc accaatacct ggcccgattt
     1561 cgatgacatt gacctgatca tcaatttcag ccgtatccac aattttttga aggatattgg
     1621 tatccgtcag gaaattttgc ccgaaggact ttttaaaggt aaaaccgtga cgctccagca
     1681 ctgccttggt cacgctataa tctgcaattc tcatctattc tcttttctat tatctgttac
     1741 tactattgta acacattccc cttgtatttt gggaatgaaa atcataccaa aaaactatct
     1801 ttcctcatga agaaagatag tcttggaaat ctttaaagag ggacttagcg cttctctcga
     1861 aacatacgtt catactcttc gaaaatctct tcaaaccacg tcagcttcac cttgccgtac
     1921 tcaagtacag cctgcggcta gcttcctagt ttgctctttg attttcattg agtatcaaaa
     1981 aaatcagctt cttgataact tcgattttcg ggtagggtca aatccacatc ctcctccaca
     2041 tcatccactg tcagttcaac cgtttgaggt aaatttttca aacgataatg cttttgcctt
     2101 tacccttcaa tactagcaaa atcaagcaaa tagccatgca ctcggatttg aaccaaaagg
     2161 agatgattgg tcatattttc catgatgagg atatcttcta aaccacctct ggcactagga
     2221 taatcagata ccctgcgaat acggattttt tcacacatga gcaagtttcc tcatatcaat
     2281 gatttcatca gccttattcc agactaggtg attatgggtc gcaatgatga taattcgatt
     2341 ttcatctttc aaatccacca agagattcat aacctcctct gaattttcag gatcaagagc
     2401 tgctgttggt tcatctgcca aaatcaaggg tggatttttc aaaatagttt ttgcaagggc
     2461 aactcgttgg gcctctcccc cagataaagt ataaattttt tgttctagat ccaaataccc
     2521 tagattaact ttttctaaag cccccacttg cctttccaaa cgttctactt ttgagatttt
     2581 ctgaccaaca aaacccaaat ccaaattttc tttgattgat tggttttcta agaggccgaa
     2641 attttgaaag agatagccca tctggtctcg aaaatactca cgagtgggaa tggtttttaa
     2701 atctttcccc tgatagagaa cccttccacc atctatcttt tctagctttc ccaagatatt
     2761 cagcagcgtc gtctttccgc ttccactctt tccgatcaag gcataaacct tgcccttctc
     2821 cagcttgaga ttcaaaccag agaaaatcgc gcggtcatta aatttctttt ccaatccttg
     2881 aatatcaatc atcttatttt cctttcaata ctgccatgct accagcttct tcttttttgt
     2941 cctgccttac taaaatcaag agggcgttaa gcgtaaacaa agctacaact agagcgctaa
     3001 tcaaaccatc tcttgtcaaa atactagata ggactaggcc aagcaagaga actcctcctt
     3061 gcgccagtaa atacttgcca tgaagctcat agattgtcat accagcaaga cgtttaatca
     3121 taagctcccg tctgaactgc tcaaaataga gaagattcat ggaatcaaat aacaagatag
     3181 ccgtagacag ggtcaaaatc gtcccaataa ggagagagta aaactccacc cgtttgtcat
     3241 ttagtacctt ggcaaaaaat agctggcttt ttaccaagta agaaaccttg tgatacagac
     3301 cttgctcttt tagagctgtg atgctgtctc catatcgatc tagtttcaag gcactattag
     3361 ccgtagttcc ccacaacatc cctgcaacag gtcttgttcc agtcgcttgc ggcgttagaa
     3421 ctacaataat cggatctttt aggtactggc gtgttgtctt gtacccatca ttatagagga
     3481 aacgttcctg tcctatttct gtaaaagcta gccttacctg tgggaggtag tgtttctgac
     3541 tcgtcacttc tacactttca gatgaaaagt tttgcaggta atctgtaaac aatccttggt
     3601 agtagacaga ctgctctcgc aagctctcag gcaagatcag cccaaactct ccctcagaca
     3661 agttttgcat cttgtccata aactctgaag aaacggtaat cttttcttct atcagatagc
     3721 gtggtgagac atagataaca ttccctgacg gtgtgtagtc actgagacga ttgccatcta
     3781 ggtccacttc tgctccatct gagaaattgt caacattgct cataatataa aaagagtctg
     3841 tattggctaa ccgttcttca gtaaatgtct gccactcacg attatcctta cgcgttcctt
     3901 cttcatcggc aaatgcacta gaccaaccaa aggatagacg gtaacggtct gaggactggc
     3961 tccatttatt gctagctctc tccatttcct gcatttcacg gtagtggggt aggagagctg
     4021 tcgcactcga tccgaccacc aatacagcta agagttgccc caccatcatc aatgtcatca
     4081 tacgtttgag agggagtttc cctttcaata gatccaccag actattttcc tgtaaaccaa
     4141 gtagatagac gacacttagt aaggtagaaa tccctgccaa ggtcaatcca taaagtagaa
     4201 gagcaatgat gaccagttgc accgttgcca taaacaaggc accttgatac cagagaatcc
     4261 ccaatcccaa aagactggat accagcactg agcagataag ctgtctcaca tcttctaaca
     4321 ctggtctgag agcaattcca aacaagctct caccagctat taagcgaatc cctgcctgac
     4381 gaagggattt gatccgataa atcagggtca gactcataaa ggtcagcaga aaaatagcta
     4441 aactcagtag caccataggg gtggccgtca atagtaagac tatcgaaaat ggatcttctc
     4501 cattcgaaac aaagccttga tagccaagtt ctttcaaggt ggtctgtaag ctcactccat
     4561 ccaaacttcc tgacacaatc aagtaagacc ctaataaatc actcgtttct gcactctcct
     4621 tggaggaaat ggtcagacct tctggaagct ctccctcacc ataagtggca taggtaaagc
     4681 gtgtttctcc agcttcattt ggctcaacga ttcgacgggc gatgagacta ttatgctcta
     4741 ccgccaagtg actcaaagcc tctgtcacct cttcgcgact agctactggt tgatttgtca
     4801 tcattcctgt cacatcaaca gaaggatagt taccaaataa aatctcttct cgatggtcaa
     4861 cggaagttat caccatataa agcgatacca ataccattga aatcaaaata aataaacgtt
     4921 tcatcatttc ctccaaaaag ttacttggtc acttagtgag aaggtgacca agtaaagcta
     4981 aacaataggg ctataccatt ctaaacatta tagtagaatg caactttctc tccccaagaa
     5041 gtacgaatcc atgcacgaga agtccctcca gcaccagcat atcccttgtc agatgaacca
     5101 gtttttctgc taacaactgt tgaactatgg tattttgaag ggtgatagta gtttgagtaa
     5161 gcaccaccgt tacctgaacc ataagtccaa acaccacctt ctgggtattg aacggctgca
     5221 gaggcaactg ctacacttcc acctaatgtt gctgcaatgc tcgctacaag agctaatttt
     5281 ttaactgttt gtttcatatt gatactcctc tatttttgat tacaagattg ttgagttact
     5341 tgctcctctt tccattatca acgttgatac tattatatta ccaaatgata ttctaaaatt
     5401 atttgttttt ctctaaattg tcaaaatagc accctgaaat gaacttttat agtcattata
     5461 ctccatcttt atcacactat caaggacatc aatgtcctta aaatctaact atttcatatt
     5521 atttttagct tatatctaaa aaaacagttc taaagacgaa ctttagaact gttttgatac
     5581 gaggacaagg acatttttgt cactgattca ctatgtttat aaatattttt tgacatcctc
     5641 ttgccattcc tctgtcaatt tctttactag aaattcatct ccaattagct gcgcaaatgt
     5701 tttagaagac tgataaaatg attcagctaa actaaaatct ctttcttctc ttaatgcttg
     5761 cttccacaaa aacataaata caagaggttt cttttgataa tcttgagttt tatccataat
     5821 ttcttgtaaa cacgatagat aatcattaaa acaatcagtt actcctacct tttcaagaca
     5881 acacattcct gaaataagag tatctctaat tataaagaag ttttctaatt tagttacctt
     5941 tttctgttcc aaaagaaaga gcattagctt ttcgatttga tttataaatt tggcaagatc
     6001 ttttctaatg agcatttgat aaaaaaacaa acgaatcaac aacatatcat ttgttgaata
     6061 aacttctttt tctatgatct gagacatatg ttcctcaagt attaactctg catattcagg
     6121 ccttctagtt ctagaagtat ccaaacacgc ctgcaatact tcacatgcta attgttcttc
     6181 tttaggaaga ttatcaaagt atgttttata aatctcttcc aaacacgctt ccttcttatc
     6241 gtactcctct tctttaccat agattggttc acgtaaaatc tggtatttca attctaaata
     6301 agcaggagga agagctgaaa aattcggcat aaggctataa actggaactc ctagccggcg
     6361 agcaatatag gctaacttgg gcaaactcgg ttgcgaagct ccactttcaa ttcgcgacag
     6421 ttgacgaact gtcagttctt gctcatctcc acaaaaatat ggacgactaa ctcctttttc
     6481 tatccgaatc tctttaattt tttgaccaat taacatcaga cgcctcctct tctacctgct
     6541 tcgaaaaata ctgtgaaact taaaaaattg taacggctac atctttccta ctcataagat
     6601 ttcatagctt cttccacttc tgccaaagta accccaaaca actctaggcg tttgaggagt
     6661 tgcttgccgt tagaatagcc gattcggaga gtctctccga gatattctct acgcttacgg
     6721 ctgtctgccc ctgctagaaa accaaggcga atcaagtcgc tacgactaat gtcaaactga
     6781 ctctcatgtt caaattgttc tgtcacctga gctagagccg ttttcaggtc ttcatagctg
     6841 gcatgctcaa ttcccagaga acgccccttg gtcttggact ttggaacagc ttcatctcgc
     6901 ttgagaaagg catgccgaac tgttggaatg gccgtcataa tcatgcgccg aatccgctcc
     6961 ccattaaaat ctgggtctgt aaagacaatg actccatgac gttggtgcag gcgctgaatc
     7021 cgctctatat cctgagcatt gatggcagaa cctcgagtct cataggtctc cacatcgaaa
     7081 taacgtttga gattgaccgt atcatcacgc ccttcaacca cgataacttg agaaattttc
     7141 tctttcatta cttgctgtcc aatccaaaaa ttcgttctgc atttgcagtc gttgctaccg
     7201 ccagctcttc tgtcgtcata ccacgcaagt cagcgataaa gtcgaccaca tagcgagtat
     7261 aggctgtttt attttcacga ccacgcttgg gtacaggtgc taagtaaggc gcatctgttt
     7321 ccaccaaaat cttgtccaaa ggtaactctt tagctgcttc ttggaggtca gttgccttct
     7381 taaaagtcac cactcctgag aaggaaatgg tcataccaag atccacaaac ttctctgccc
     7441 actcaagcgt ccctgaaaat gaatgcatga taccaccacg aggaccaacg ccctcactct
     7501 tgataatctc ataggtatct tccagcgcat cacgggtatg gacaacaaaa ggcaaatcca
     7561 agtccttaga tagctgaatc tgacggcgaa aaacctgctc ctgcacctct ttgggcgctg
     7621 tcatccaatg gtaatctaag ccaatttcac ctaaagccac aaccttggga tgttttaact
     7681 tatccaacaa gtaagcctca acttcctctg tataagtacc agcttctgta ggatgccaac
     7741 caatagtcgc atagagctga tcatactcat ctaccaactc caaggcacgc tcaatcgtcg
     7801 gtttatcaaa accaacaata ttcatctgtg tcacacccat ctcagcagcc aaggcaattt
     7861 cttctgcctc acgacctgca aattcttcta cattcaagtg tgtatgtgta tcaaaaatca
     7921 tctcttctaa cctcgttttc tatcttctat tataccaaaa aagaggaggg gcacctaatt
     7981 tttcggtttc ccctcctctc ttcaatagag agctattctg ctatcttttc tatccgatat
     8041 tgcccatctc ctattccaca gttagagaca gaagagattg gctacattga tagaatattc
     8101 ctattcagct aaagtatgtg attatcttgg gttctcactt ttctcaccca tcactaggat
     8161 tgatgacttc tcaaaatccc ccttataatc taactttaag tagagaatag aattttctag
     8221 ctgttctatt ttctacgtct agtctttctt cctagcaaag caaaaccact tgttgccacc
     8281 actagaccaa gactagcaag tgctgcactt gatgctgaac ctgtgttagg taattgtttg
     8341 ccagcaggtg actggtaggt aacatcttgc attgttcctg cagttgcttg cggtgcactt
     8401 gttgtcttag gagcagcatt aggagccact ggtctcttag gaactgtaac catgacaatg
     8461 gcttgcactc caatcgtttc tgcttccttc acatcttcca tagttttcgc attttcaatc
     8521 tctttgagag ctgcttgttt ttctgcttcc actcttgcta aaagttctgc tttttcttta
     8581 tcagaaagcg gtgcgccttt gatttcatcc tgcttgtcct tggctgcttt ttcaatggca
     8641 tttttagctg attctttttc agtagctaat tgcttagaag cttgtgattg agctgagttt
     8701 tgtgaacctg tgtgagctga tccacccgcc tggctaccac cgcctgcatt cgcattagca
     8761 tctgagcttc cagtattgcc tcccttatct ccacctggtg tattttctag cttatactct
     8821 ggtgtagctt cttgagttac agattcacct ttcgcagatt gttgaactgt atcttttcct
     8881 agaatcgtca ctactgaacc atctgggaat gtgattgttg ctgtaccatc tccagctaca
     8941 ttgattgtcg ctccgtctaa tgctgaacca tttgcttgta aaatagcaac cttaactttt
     9001 tcttcatctg ttaaatgagt tatatctttt acatctactc tttgagcagg tgctttaaca
     9061 gttgattttt cagcagtcgg tttagtgcta tcttgtgctt ttggatcttg tgctaccaat
     9121 tgatttcctg gaattgtaat agctgaacca tctttaaaga cgattgtcgc tgtgccatct
     9181 ccagctactt caactttata gtaaaatgaa ataataacag gacaaatcga tcaggacagt
     9241 caaatcgatt tctaacaatg ttttagaaat agaggtgtac tattctagtt tcaatctact
     9301 ataacatcgc ttccgaatcc atttgcatct actgaacttt tttacaaaaa taaaaatcag
     9361 tagactcgag ttcctactga tttttgatag aattcgtttg gattatgcag ccaaaacttt
     9421 gcgtccttta cgacgacgag ctgccaatac gcgacgacca tttttagttg acatacggtt
     9481 acggaatccg tgtttgcgcg cacgacgaag tttacttggt tgataagtac gtttcacgat
     9541 gaatacctcc tcatagattt tgtattcgtt tagccggcta taaagtgatc agttactaaa
     9601 catactttac tattctatct gattctttca cttttgtcaa tagctctagg caaatgtttc
     9661 cagctttttc gaatgcaagc cctatctacg atactgtttc aagaaacgag tcatcttttc
     9721 ttggatttgg gctaactcat caacacgagg aaggtaaacg atacggaagt ggtctggttc
     9781 ctgccagtta aagcctcgac catgaaccaa gagaaccttt tcctgcttca agaaatcaag
     9841 gacaaactgc tcatcatcat cgatacggta catattgcgg tcgatttttg ggaagatata
     9901 gagccccgcc ttgggtttaa cggcagacaa acctggaata tcttgaatgg cattatagat
     9961 gaaatttctt tgctcgtaga ttcgtccacc aggaagaagc aattcatcga ctgattggtg
    10021 accccccaag gaagtttgta cgacttgttg agccaaaacg ttagagcaaa ggcgcatatt
    10081 ggacagcata ttgagccctt cgatatagcc cttaacatga gtcttagggc cagacaagac
    10141 catccaaccc acacggaaac ctgctatgcg gtgggatttt gacagaccat tcatgctgac
    10201 acagaagaca tctggtgcca agctcgccac aggcgtatgc acatgtccgt ccattaccat
    10261 gcggtcatag atttcatccg caaagataat caaatcg
//
