LOCUS       NZ_FDFR01000001        10288 bp    DNA     linear   CON 22-DEC-2024
DEFINITION  Streptococcus pneumoniae strain 2842STDY5753301, whole genome
            shotgun sequence.
ACCESSION   NZ_FDFR01000001
VERSION     NZ_FDFR01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2016) SC, Wellcome Trust Sanger Institute, CB10
            1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900022985.1-RS_2024_12_22
            Annotation Date                   :: 12/22/2024 02:12:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,313
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,086
            CDSs (with protein)               :: 2,086
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 71 of 173
            Pseudo Genes (incomplete)         :: 93 of 173
            Pseudo Genes (internal stop)      :: 58 of 173
            Pseudo Genes (multiple problems)  :: 41 of 173
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:29:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 343645
            Orig. end                         :: 353933
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FDFR01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10288
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10288
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10288
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5288](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5288)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     assembly_gap    52..168
                     /estimated_length=117
                     /gap_type="unknown"
                     /linkage_evidence="unspecified"
     gene            <169..465
                     /locus_tag="A1O50_RS13345"
     CDS             <169..465
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /locus_tag="A1O50_RS13345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transposase"
                     /protein_id="WP_228111064.1"
                     /transl_table=11
                     /translation="FHFQNKEPEKFFGLIEDNLKQVHPIFQTVFKTFLKDKEKIINALQ
                     LHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(<781..1173)
                     /locus_tag="A1O50_RS01820"
                     /old_locus_tag="ERS409084_00357"
                     /pseudo=""
     CDS             complement(<781..1173)
                     /GO_function="GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269502.1"
                     /locus_tag="A1O50_RS01820"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ERS409084_00357"
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSKEKVILAYSGGLDTSVAITWLKKDYDVVSVCMDVGEGKDLDFI
                     HDKALKVGAVESYVIDVKDEFATDYVLVAHQSHAYYEQKYPLVSALSRPLISKKLVEIA
                     HQIGATTIAHGCTGKGNDQVEYQIVVA"
     gene            complement(1190..1996)
                     /locus_tag="A1O50_RS01825"
                     /old_locus_tag="ERS409084_00358"
     CDS             complement(1190..1996)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /locus_tag="A1O50_RS01825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS409084_00358"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_061751094.1"
                     /transl_table=11
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVALN
                     PDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKADLA
                     ISGVSKTDERSKVFDFSIPYYTAKNKLIVKKSDLTTYQSVNDLAQKKVGAQKGSIQETM
                     AKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVSKGFVENNPDLAIADLNFEKEQ
                     DDSYAVAMKKDSKKLKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(2203..2844)
                     /locus_tag="A1O50_RS01830"
                     /old_locus_tag="ERS409084_00359"
     CDS             complement(2203..2844)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /locus_tag="A1O50_RS01830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS409084_00359"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_061751095.1"
                     /transl_table=11
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLLN
                     LLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLMLAL
                     KYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLILADE
                     PTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRIIELGKGE"
     gene            complement(2841..4949)
                     /locus_tag="A1O50_RS01835"
                     /old_locus_tag="ERS409084_00360"
     CDS             complement(2841..4949)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /locus_tag="A1O50_RS01835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS409084_00360"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_061751096.1"
                     /transl_table=11
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDLY
                     LKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLDFT
                     TDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEMDKD
                     RIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQPMMEM
                     KRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPMLWLSHF
                     LLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTALLIGVGR
                     SLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFYQDLVEKT
                     QAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTGIKKVILMP
                     ASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEDMDVEIAYYEGDWSFFPYSDKRKENL
                     SNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKLSDGNYLKFSS
                     IQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRDIITKKFMGWKL
                     VDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVLGLASRMEKRSLV
                     ELLKGGIL"
     gene            complement(5001..5288)
                     /locus_tag="A1O50_RS01840"
                     /old_locus_tag="ERS409084_00361"
     CDS             complement(5001..5288)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /locus_tag="A1O50_RS01840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS409084_00361"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_001230165.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 2.6e-31,
                     bitscore: 102.8, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFGY
                     SDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
     misc_feature    5334..5350
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 23.57"
                     /tool="antismash"
     misc_feature    5609..5622
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 25.42"
                     /tool="antismash"
     gene            complement(5675..6262)
                     /locus_tag="A1O50_RS01845"
                     /old_locus_tag="ERS409084_00362"
     CDS             complement(5675..6262)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108787.1"
                     /locus_tag="A1O50_RS01845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS409084_00362"
                     /product="hypothetical protein"
                     /protein_id="WP_001108797.1"
                     /transl_table=11
                     /translation="MNYNLKYLLSGIVVLVFIGFLVWMRYFNQRKEEEHSDVSFEARLD
                     SEVKTLYKQLGLGEEPHYFLAYRYLHPWFDLAILPPTVEIFLTKIALVMVTPDELLIRN
                     LGNGLTFTSEHHRDLRQGLIRIPKSEMKEFEIRNWKKFFVFGDFLTIKTSQHSYYLQVR
                     DDGLQKGSLSTKHFSDLKSQDFLGLLTDKRTF"
     gene            6585..7448
                     /locus_tag="A1O50_RS01850"
                     /old_locus_tag="ERS409084_00363"
     CDS             6585..7448
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268144.1"
                     /locus_tag="A1O50_RS01850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS409084_00363"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_001268178.1"
                     /transl_table=11
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGNVISRTSLSKIESGKATPKY
                     ENMEFLLRQINMSFEEFDYICHLYQPSQRTEIMQTYLNMTSIIGSNSLVHFFETCQDYL
                     KTHHDLPIEEIRDMLEIVIHIRQHGTEQLSNQVKQTVTKLWEKIEKQDTWYENDLKILN
                     TILFSFPIDHLHLITGKILQRLEVYKNYQHLYDLRMAILLNLSTIYLYHQDKNMCRQIC
                     YTLLEDAKKKKHYDRLAICYVRIGICRDDARLIQKGFSLLELTEETSMLSHLKKEVEIY
                     SQPKEI"
     gene            complement(7500..8087)
                     /locus_tag="A1O50_RS01855"
                     /old_locus_tag="ERS409084_00364"
     CDS             complement(7500..8087)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /locus_tag="A1O50_RS01855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS409084_00364"
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="WP_000836450.1"
                     /transl_table=11
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLSE
                     IAETHNTTVEKLAENNHIDNIHLIYVGQELVIDGPVAPVATPAPATYAAPAAQDETVSA
                     PVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYS
                     AENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     misc_feature    complement(8157..8173)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 26.23"
                     /tool="antismash"
     gene            8329..9000
                     /gene="sdaAB"
                     /locus_tag="A1O50_RS01860"
                     /old_locus_tag="ERS409084_00365"
     CDS             8329..9000
                     /EC_number="4.3.1.17"
                     /GO_function="GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /GO_process="GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sdaAB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /locus_tag="A1O50_RS01860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS409084_00365"
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="WP_000838912.1"
                     /transl_table=11
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPTE
                     VEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKDSN
                     APHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDIPGM
                     IALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHNVNFF
                     K"
     misc_feature    complement(9009..9025)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 24.79"
                     /tool="antismash"
     gene            9009..9881
                     /gene="sdaAA"
                     /locus_tag="A1O50_RS01865"
                     /old_locus_tag="ERS409084_00366"
     CDS             9009..9881
                     /EC_number="4.3.1.17"
                     /GO_function="GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /GO_process="GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sdaAA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /locus_tag="A1O50_RS01865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS409084_00366"
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="WP_000500038.1"
                     /transl_table=11
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLMER
                     NLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNEHN
                     AKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASISGA
                     EGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEVPCVK
                     RNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLATTPTGR
                     RLQKEIFGE"
     misc_feature    10249..10265
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.51"
                     /tool="antismash"
ORIGIN
        1 aagcactact ggaaactcat tcaacaggat agccgtaaac tgagtgataa gnnnnnnnnn
       61 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt tcacttccag
      181 aataaggaac cagagaaatt tttcggactt attgaggaca atcttaagca ggttcatcct
      241 atttttcaga ctgtctttaa aaccttcctc aaagataaag aaaagattat caacgccctt
      301 caactacact attctaatgc caaactggaa gcgaccaata atctcatcaa acttatcaag
      361 cgcaatgctt ttggttttcg aaactttgaa aacttcaaaa aacggatttt tatcgctttg
      421 aacatcaaaa aagaaaggac gaaatttgtc ctttctcgag cttagctgac ttcaacccac
      481 tacagttgac aaagagcctt atttgttcgt tagttcttaa taccagccat tgttaaacca
      541 gaagttttta gctctgccaa gccacatagt gtgtttacta ctatgtgtcc atgtcaccat
      601 gaatcgccga acgatttaac gagaaagtat gacttttacg tttatcccaa ctcaattatg
      661 acattttttt caaaagtcaa tatatctcac tttttcaacg acaagaaaga ggctgataat
      721 ctaccaacct cttgttctga acccatcact ccatcacttt ttagcttcat tcgctttctt
      781 agcgactaca atctggtatt cgacttggtc attcccctta ccggtacaac catgagcaat
      841 tgtagtcgct cctatctgat gcgctatttc aaccaatttt ttagaaatca gagggcggct
      901 caaggcagat accaagggat acttttgttc ataataggca tgtgactgat gagccactag
      961 cacataatct gtagcaaatt cgtccttaac atcaatgaca taagattcta ctgccccaac
     1021 cttaagagcc ttatcatgga tgaaatctaa gtctttccct tcacccacat ccatacaaac
     1081 tgaaacaaca tcatagtctt tctttaacca ggtaatagca actgatgtgt ccaaaccgcc
     1141 agaataggcc aaaattactt tttctttact catgttcttt tccttctttc tatttttcaa
     1201 tggatgcttt aaaggcatcc tcaatgagtt tgtctaattc cccagactcc ttcaactttt
     1261 gaatggtttt atcgactgcc tctttcaatt tcttgctatc ttttttcatg gctaccgcgt
     1321 aggaatcatc ttgctctttt tcaaaattga ggtctgcgat tgctaaatca ggattatttt
     1381 ccacaaatcc cttggaaaca ggttcttcaa agataacggc atccacttgt cctgatttta
     1441 aatctgtgat taaattccca tttttaggca gagatacgag ggaagaattt tgtagcaaat
     1501 ctttcgccat cgtctcttga atcgaacctt tctgcgctcc aacctttttc tgcgccaagt
     1561 cgttgacaga ctgataagta gtcaagtcag attttttgac aatgagttta ttttttgcag
     1621 tatagtaggg aatggaaaag tcaaacacct tgctccgttc atctgtctta gaaacacctg
     1681 atatggcaag gtcggctttt cctgattgaa cactagccag tacattatca aaactcatgg
     1741 gagatagttc caattcgaca cctagttctg ttgcgatagc cttggctaat tcgatatctg
     1801 aacccacaat ctgatttttc ccatcaacca ctttttggta ttcaaatgga gcaaaatccg
     1861 gattgagggc tacaaccaat tttcctttgg acttgatggc ttcaattcca gccgattgat
     1921 tgttgctaca agcaaatagt aggggaagca tcactaaacc aaacatcgtc atcaacacct
     1981 tcttcatctt attcataata aagcccactt tatgtttatt ccttttgttt gtataaacaa
     2041 tacatctata tgcatccttt gtcaagccta aattgtaatt tttttcaatt taaaacagaa
     2101 aaacccagga aaatgatata aaaatatcat tcctaggcgt atttatgcta tttatctctg
     2161 ggaaacatta gtattcagtc agtcaaaata agcagatcta actcattccc ccttgcccaa
     2221 ttcaatgatt cgatgacatt gttgggctac ataagcatcg tgggtcacaa taatgactgt
     2281 tttcccttct cgattcatct ctaagagaaa cttcaagacc aaatctctat tttcaggatc
     2341 cagcgaacct gtaggttcat cggctaaaat cagctggctg ggttttaaga tggctctagc
     2401 aactgcaatt cgttgttgtt cgcccccaga caactcggag accctttgat gcaaagtagc
     2461 tgacaaacct actctctcta aaatctcttc cacctttttg agcttgtctt tcttaggcaa
     2521 tttcacatat ttcagcgcca gcatgagatt gtactcgacc gtttcatcat caatcagggc
     2581 aaaattttga aacagataag agatatgttc acggattatt gtttgcgact tagcagaatt
     2641 aaccgctaga tttgtctgac caaaaatctc ataccgtccg ctataatcac catctatcaa
     2701 acccaataaa tttaacaagg tcgacttccc actaccactc ttaccaacaa tagctaccaa
     2761 atccccctga tcaatcctga gagataagtt atccaaaatc acttttcccc caatggtttt
     2821 ggtaatattt ttcaactcaa tcataagatg ccccctttca ataactctac tagacttctt
     2881 ttctccatcc tagaagctaa gcctagcaca aatagtatat ccagacatgt aaaacctgca
     2941 aacagtagaa gtggtaagaa cgcatgggca aagaaaatca agactagaag agggaaacta
     3001 tagcccagca agagcagaac gaggagagga cggtagcgat cgaccagttt ccaccccata
     3061 aacttcttgg taatgatatc cctgcgcttc aataagaaag ttgttactag taagaagtag
     3121 gaaatcatca tgctaaggag accaaacaaa gcaaagagta ggttaaaatt ccgaacagca
     3181 tctcgataag aatccacttt ctcttgttga atggcttgaa tagatgaaaa ttttaaataa
     3241 tttccatctg acaatttctc aactaactct gtaatctctt tttgatgttg aaccgtattt
     3301 tcaattttaa tcggattatt taagccagtt gttgacaggg aggctttctc atcccacatc
     3361 atatcagaat cattgaccaa gctaataatt ggattggaga gattttcctt tcgcttatca
     3421 ctatatggga aaaatgacca atctccttca taataggcaa tctcgacatc catatcctct
     3481 atcgttcgtt tttgctgctc ttcatacttc atcgaatgaa aggcaattaa cttccccaag
     3541 agctgatttt tatcttcttc acctttcgta cttgctggca tcaaaataac ttttttaata
     3601 ccggtatctg gtagcttgaa tcccttgctc tttagaaaat tgcgattggc atagtaaaca
     3661 tccaccgtat ctgttaactg atattgctga atctgttctg attggacaaa attttttaca
     3721 ggaagactgc tactctgcac atagcccgcc tgcgtttttt ctaccaaatc ctgataaaat
     3781 cgatagaaat aatctgtaga tttccctgac cctgctagct cttcttgcca cagattatca
     3841 ttgagtttga aggtttctaa ggtcaggtaa ttaccttgac ttacccactg ttgctgataa
     3901 gcaagttctt tgttttcttg ttctaaactt ctgcccaccc caatcagtaa ggccgtcagt
     3961 aaaatagttg tccctatttt catcacataa ttgaagataa gaccaaattt gaaagatgaa
     4021 aaacctttca gcagagagct gattgtcatt ttttggatta agaggtaagt caaccaactg
     4081 ataaagagat aaagctgcaa cagcaaaaaa tgagacaacc acagcatagg aaacaaatct
     4141 tttggcttat aatcaagcaa gaaaaacacg cctagattga tcacaagagc cccacctagg
     4201 aggaggtaaa ggttgccttt tacaacatca gctaaaacag ccctatcttg aaaaccaagt
     4261 aatttttgta ccccaactct tttcatctcc atcatcggtt gatacactgt cactaacaca
     4321 agaagcaaaa tagccaagac aaaaacaatg gcagataaaa gcaaatctcg atttatgact
     4381 tccactgcac ttttgtaggt cggctctagc aaggtcgcct ggtctatctt gaaaaaatcg
     4441 ctccatttct gtacaatcct atccttgtcc atctcttgtg tagaagttat cgtatagcga
     4501 ccatttaaac tacgagatgt atccttgata taggtttgaa aagtcataag ctgaataggt
     4561 ttggctttta gaaaggtcgg aatcgtacca agtttattgg aaatttcttt attactatag
     4621 actccttcac catctgtggt aaaatcaaga gaagaaatcc caaactcttg gtaggggaag
     4681 gtatctttat caaaaacacc agacttgacc acctcatcac cactgtctgt tttgatgatg
     4741 gagactttat actcctttga tacatcctca aaaaatcgaa gaacagacgc tgcaggttcg
     4801 ttaatatctt tcaaatacaa atccaaagaa tctacagtct ttcccatttc ttgaatccga
     4861 accacttctc gtgtatagtt tacattaaaa acaaaaaaag tggtacacag aagcaggagt
     4921 aacatacaga aattactgat ttttttcata aaagtgcctc cactacagtt ttccctctcc
     4981 aaataggaga gggaagaatt ttactcaaat ccatagaaat attccattcc tgttggcgga
     5041 atcttggtga gggaagctct agcccatgcc tcaggttttg cataatcctt agaggttcta
     5101 cccccatgtc taacagttgc tgtatgatat cgagtagaat gtaagtaatc agaatatcca
     5161 aaagttcctg tccaacctac tccatagttc cattgaccac cctcaaccca tactgctaaa
     5221 gcatttgtag tagcaagtat accacatacc attaaagatg caacaccaat tttaactaat
     5281 tttctcatga tttatttctc ctattatcga gagctgttcc gtaaaaacag ctatagtaat
     5341 ttatcaacaa gtatcgtagt ccattggact caccttcctt ctatctcatt tcatttttaa
     5401 acaacgaact tactcttaac tagaagaata gttctcttct gtatcaggtc tttgaacaac
     5461 cagagtagta ttaatcacac tctaactatt actcaagaat cagcccaata aacaatatgg
     5521 atagcgcttt caaattttta aactcttatc ccatcctttt atctatataa taagtgaaaa
     5581 tataataact gtcaagtaat cgaagtgaat tttataaaaa aaattacaag ccaaatttgc
     5641 aaagtttaca ataaacaact agggcaatcg tctatcagaa tgtccgttta tctgtcaata
     5701 atccgagaaa atcttggctc ttcaagtctg agaaatgttt ggtagaaagg cttccttttt
     5761 gaagtccatc atctctaacc tgtaaataat agctatgttg gcttgtttta attgtcaaaa
     5821 aatcgccaaa tacaaaaaat tttttccagt tacgaatctc aaattctttc atctctgatt
     5881 ttggaatgcg gataagtcct tgccgcaaat cacgatgatg ctcacttgtg aaagtcagcc
     5941 cattcccaag atttcttatc agtagttcat ctggagttac catcaccagc gctattttag
     6001 ttaagaaaat ttcaactgtt ggtggtaaaa tcgccaaatc aaaccaagga tgtaggtagc
     6061 gatacgctaa gaaataatga ggctcctcac ccaatcctag ttgtttatat aaggtcttga
     6121 cctcactatc taacctcgct tcaaaagaca catccgaatg ctcttcttcc ttcctctgat
     6181 taaaataacg catccagact aggaaaccta taaagactag gacaactatt cctgataaaa
     6241 gatattttaa attgtagttc atcacttaac ctcctgattc acgcttctgc ctaaaacaga
     6301 ggcctattta ttttgaaaat agttccatca taactttaaa taagacgggt gtcattaaaa
     6361 ttaggataat tcctatcaca aatatcatga ctactcgaaa aagttcctta tttttaaaca
     6421 tttttccacc ccttttctag ctacatcata gtactcatta accccatatt aaaattacac
     6481 caaacttatt ttatctttct tttttacttt ttatattact atgatacaat atttttaata
     6541 ggattttatt atttgttaac ttttttcacg aaaaggagtg aatcatgcgt tatgatttcg
     6601 gaaaggtcta taaagaaata cgcgagtcaa aaggattgac ccaagaagag gtctgtggaa
     6661 atgtcatctc aagaactagc ctatcaaaga ttgaaagtgg caaagcaact cccaaatatg
     6721 aaaacatgga atttcttctc cggcaaatca atatgagttt tgaagagttt gactacatct
     6781 gccatctcta tcaaccaagc caacgaacag aaatcatgca aacttatctc aatatgactt
     6841 ccatcattgg tagtaatagt ctagttcatt tttttgaaac atgccaagac tatctcaaga
     6901 cccaccacga cctgcctata gaagaaataa gggatatgct ggaaattgtc attcatatcc
     6961 gtcaacatgg tacagagcaa ctgtcaaacc aagtgaaaca gactgtcaca aaactttggg
     7021 aaaaaattga gaaacaagat acatggtatg aaaatgacct aaaaatcctc aataccatcc
     7081 ttttcagctt tcccattgat cacctccatc tcattactgg aaaaatcttg caacgcttag
     7141 aagtctataa aaactaccaa catttatatg acttacgaat ggcaatccta ctcaatctgt
     7201 ccaccattta cttataccat caagacaaaa atatgtgtcg acaaatctgc tatactttac
     7261 tagaggatgc caagaaaaag aagcactacg atagacttgc tatctgctat gtccgtatcg
     7321 ggatttgtag ggatgacgct agacttatcc aaaaaggttt ctcacttctg gagctgaccg
     7381 aggaaacttc tatgctgtct catctcaaaa aagaagtaga gatctattct caaccgaagg
     7441 aaatataaaa aagtcgaggg atttcctcga ccttttcata ttattctgct cgttatttct
     7501 taataccagc cgttattaag ccagaagttt ttagcggctg tccatgaacc gtaacgtcct
     7561 gcaacatagg catctgctac acgttcttgg ttttcagctg agtagtcacc gttcaggtat
     7621 gaatctgtta attggtaacg tccgatgtaa cgtccgtttg tagctgtgta gctaccgcct
     7681 gattcttttt gagcgatcca ttctttggct tctgcttcag atccgcttac agttgaaaca
     7741 actgtttcac ttactactgg agtttctgct actggagctg aaacagtttc atcttgagcg
     7801 gctggtgccg cataagtagc tggcgctggt gttgcaacag gcgctacagg gccatcgata
     7861 accaactctt gaccaacata aatcaaatga atgttatcaa tgtggttgtt ttctgccaat
     7921 ttttcaactg ttgtgttgtg agtttcagcg atttctgaaa gtgtatcacc ttctttaaca
     7981 gtgtaagttg atgattcttg agcagataca aatgatggag caaatactgc aaacaaggca
     8041 gctactcctg caagagttgc tttaatcttt ttagttattg atttcatatt cgaaaattct
     8101 ccttctttct atagttctat catacccttt aaatattacc gtttcttgac acttttatgt
     8161 agaaatatta caaaattatt ttttatttcc ctaaataagg tgttttttgt cataaaagat
     8221 actattttta tgctatttga ggtattagaa aggttttcat ccacaattaa agccaagccc
     8281 ctcattcttt gatataatag agataagaat ttttataagg ggaaactgat gaaatcactt
     8341 cgttttcaat ctgtctttga tatcatcgga ccagttatga ttggcccatc tagtagccat
     8401 actgctggtg ctgttcgtat tgggaagatt gtctcttcca tttttgatga tactccgaca
     8461 gaagttgaat tccaactttt taactcattt gccaagacct atcgtggtca cggaacagac
     8521 ctagcccttg ttgcaggtat tttaggcatg gatacagatg atcctgaaat tccaaacagt
     8581 ctggagattg cccacaagcg tggtatcaag attgtctgga ccattcagaa agacagcaat
     8641 gcccctcacc ctaacaccac taaaattact gttaaaaatg cccacaagac catcagcgtg
     8701 actggtattt ctatcggtgg aggaaatatt caggtaaccg aactcaatgg ttttgccgtc
     8761 tctctcaata tgaatacacc gactatcatc atcgttcatc aagatattcc aggtatgatt
     8821 gcccttgtta cagaggcgct ttcccgctat ggtatcaata tcgcccagat gaatgtcact
     8881 cgtgaaaaag ctggtgaaaa agccattatg attatcgaag ttgacagtcg caactgtgat
     8941 gaggctatcg aagaaatccg aaaaatccct catctccaca atgtcaattt ctttaaatag
     9001 gaggaagcat gttttattct atcaaagaat tggtcgagca agcagatctg gactttcaag
     9061 gaaatgtcgc agaactcatg attacaacag agtttgaatt gaccggtcgc gaacgtgaag
     9121 aagtcttcct tctcatggaa cgcaatctgg aagttatgaa agcctctgtc caacttggcc
     9181 tcaatgaaaa taaatctcgt agtggcctga caggtggaga tgctgccaaa ttggatcact
     9241 acatcgaaaa cggaaaaact ctgtcagatt atacgattct ctcggctgcc cgaaatgcca
     9301 tcgcagtcaa tgaacacaat gccaaaatgg gcttggtctg tgccactcca accgctggaa
     9361 gtgctggctg tctgccatcc gttctcactg ctgctattga aaaattagac ctcagccacg
     9421 agcaacaatt ggatttcctc tttgctgctg gtgcctttgg actagtcatc gcaaacaatg
     9481 cctccatctc aggtgctgag ggtgggtgtc aagctgaagt tggttcagcc tctgctatga
     9541 gtgctgccgc cttgactctg gctgcaggtg gaacacctta tcaggccagt caagctattg
     9601 cctttgtcat taaaaatatg ctaggcctca tctgtgaccc tgttgcaggt ttggtcgaag
     9661 ttccctgtgt caaacgtaat gccatgggag ctagctttgc tttcatcgca gcagacatgg
     9721 ccttggcagg tatcgaatct aaaatccctg tggatgaagt gatcgatgcc atgtaccaag
     9781 taggagcaag catgccaact gcctttcgtg aaacagctga aggtggactc gctaccaccc
     9841 ctactggtcg tcgcctccaa aaagaaattt tcggagaata agcttatcaa ataggagaaa
     9901 ccatgacctc tatcacagcg atttttttcg atttggatgg aaccctcgtt gatagttcta
     9961 tcgggattca caatgccttt acctatacct ttaaagagct gggggtgcct agccctgatg
    10021 ccaaaactat tcgtggtttt atgggaccac ctctcgaaag tagttttgcg acctgcctgt
    10081 ccaaagacca aatttctgaa gccatgcaga tatatcgttc ttactataag gcaaaaggca
    10141 tctatgaagc tcaactcttt cctcagatta tagacttgct tgaggagtta tcaagcagtt
    10201 atccactcta catcaccacg acaaagaata catctactgc tcaagacatg gcaaaaaact
    10261 tggaaatcca tcatttcttt gatggcat
//
