GCF_029395415.1 - 3 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_CP113914.1 (Bifidobacterium sp. ESL0798)

Region	Type	From	To
Region 1	T3PKS	438,699	479,898
Region 2	terpene-precursor	651,564	672,535
Region 3	lanthipeptide-class-i,terpene-precursor	1,402,031	1,445,232

NZ_CP113914.1 - Region 1 - T3PKS

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Location: 438,699 - 479,898 nt. (total: 41,200 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase reductase

OZX74_RS01800: hydroxymethylglutaryl-CoA reductase

smcogs: SMCOG1187: hydroxymethylglutaryl-CoA reductase

OZX74_RS01895: 3-hydroxyisobutyrate dehydrogenase

smcogs: SMCOG1100: 3-hydroxyisobutyrate dehydrogenase

Transferases: acetyltransferase

OZX74_RS01795: acetyl-CoA acetyltransferase

smcogs: SMCOG1124: acetyl-CoA acetyltransferase

Isomerases: racemase

OZX74_RS01875: mandelate racemase/muconate lactonizing enzyme

smcogs: SMCOG1268: mandelate racemase/muconate lactonizing enzyme

OZX74_RS01880: mandelate racemase/muconate lactonizing enzyme

smcogs: SMCOG1268: mandelate racemase/muconate lactonizing enzyme

Ligases: synthase

OZX74_RS01805: hydroxymethylglutaryl-CoA synthase

smcogs: SMCOG1043: hydroxymethylglutaryl-CoA synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000866.5	0.47	other (other)	polyhydroxyalkanoate	Burkholderia sp. DSM 9242
BGC0000867.3	0.46	other (other)	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0002561.3	0.42	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0002362.3	0.40	PKS	loseolamycin A1, loseolamycin A2	Micromonospora endolithica
BGC0001080.5	0.37	other (phenazine)	endophenazine A, endophenazine B	Streptomyces anulatus
BGC0000286.3	0.36	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0001078.5	0.35	PKS, terpene	furaquinocin B	Streptomyces sp. KO-3988
BGC0000916.5	0.34	other (other)	molybdenum cofactor	Staphylococcus carnosus
BGC0001076.5	0.34	other (nucleoside)	liposidomycin B, liposidomycin A, liposidomycin C, liposidomycin Z, liposidomycin H, liposidomycin G, liposidomycin L, liposidomycin M, liposidomycin K, liposidomycin N	Streptomyces sp. SN-1061M
BGC0000872.5	0.34	other (other)	A-90289 A, A-90289 B	Streptomyces sp. SANK 60405

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000866.5	0.47	other (other)	polyhydroxyalkanoate	Burkholderia sp. DSM 9242
BGC0000867.3	0.46	other (other)	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0002561.3	0.42	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0002362.3	0.40	PKS	loseolamycin A1, loseolamycin A2	Micromonospora endolithica
BGC0001080.5	0.37	other (phenazine)	endophenazine A, endophenazine B	Streptomyces anulatus
BGC0000286.3	0.36	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0001078.5	0.35	PKS, terpene	furaquinocin B	Streptomyces sp. KO-3988
BGC0000916.5	0.34	other (other)	molybdenum cofactor	Staphylococcus carnosus
BGC0001076.5	0.34	other (nucleoside)	liposidomycin B, liposidomycin A, liposidomycin C, liposidomycin Z, liposidomycin H, liposidomycin G, liposidomycin L, liposidomycin M, liposidomycin K, liposidomycin N	Streptomyces sp. SN-1061M
BGC0000872.5	0.34	other (other)	A-90289 A, A-90289 B	Streptomyces sp. SANK 60405

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.31 out of 18.57

Confidence: strong

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.53 out of 26.06

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 20.72 out of 27.78

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.96 out of 20.97

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.07 out of 23.79

Confidence: medium

Additional weak hits (21):

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 19.20 out of 26.12

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 22.51 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 20.43 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.90 out of 28.14

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 20.01 out of 31.73

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.65 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 19.61 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 18.62 out of 26.06

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.47 out of 29.25

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.36 out of 29.25

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 21.11 out of 26.12

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 18.70 out of 26.12

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 23.36 out of 31.73

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 20.88 out of 29.38

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 23.56 out of 32.93

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.38 out of 32.93

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.38 out of 21.84

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.77 out of 26.06

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 22.08 out of 35.63

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.70 out of 29.25

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.67 out of 28.19

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CcpA	Catabolite control protein A
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
IolR	Regulator of the inositol catabolism
MogR	Repressor of flagellar motility genes
ToxT	Regulator of MSHA biosynthesis

NZ_CP113914.1 - Region 2 - terpene-precursor

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Location: 651,564 - 672,535 nt. (total: 20,972 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: synthetase

OZX74_RS02750: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000757.3	0.51	saccharide	capsular polysaccharide	Avibacterium paragallinarum
BGC0000756.3	0.50	saccharide	capsular polysaccharide	Avibacterium paragallinarum
BGC0000513.3	0.50	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0001969.3	0.46	terpene	asperterpenoid A	Talaromyces wortmannii
BGC0000521.3	0.44	ribosomal	lacticin 481	Lactococcus lactis subsp. lactis
BGC0002306.2	0.43	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0001863.4	0.42	ribosomal, terpene	bacillicn CER074	Bacillus mycoides
BGC0001861.4	0.42	ribosomal	bacillicin BAG2O	Bacillus cereus BAG2O-1
BGC0000558.5	0.42	ribosomal	sublancin 168	Bacillus subtilis subsp. subtilis str. 168
BGC0000740.3	0.42	saccharide	capsular polysaccharide	Streptococcus agalactiae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000757.3	0.51	saccharide	capsular polysaccharide	Avibacterium paragallinarum
BGC0000756.3	0.50	saccharide	capsular polysaccharide	Avibacterium paragallinarum
BGC0000513.3	0.50	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0001969.3	0.46	terpene	asperterpenoid A	Talaromyces wortmannii
BGC0000521.3	0.44	ribosomal	lacticin 481	Lactococcus lactis subsp. lactis
BGC0002306.2	0.43	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0001863.4	0.42	ribosomal, terpene	bacillicn CER074	Bacillus mycoides
BGC0001861.4	0.42	ribosomal	bacillicin BAG2O	Bacillus cereus BAG2O-1
BGC0000558.5	0.42	ribosomal	sublancin 168	Bacillus subtilis subsp. subtilis str. 168
BGC0000740.3	0.42	saccharide	capsular polysaccharide	Streptococcus agalactiae

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

OZX74_RS02750: PT_FPPS_like

Domain 1 (31 - 272): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 17.13 out of 18.57

Confidence: medium

Additional weak hits (3):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.52 out of 29.38

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 22.75 out of 30.98

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 23.11 out of 33.14

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
ToxT	Regulator of MSHA biosynthesis

NZ_CP113914.1 - Region 3 - lanthipeptide-class-i,terpene-precursor

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Location: 1,402,031 - 1,445,232 nt. (total: 43,202 nt) Show pHMM detection rules used

lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))
terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acyltransferase

OZX74_RS06035: acyltransferase

smcogs: SMCOG1141: acyltransferase

Lyases: cyclase dehydratase

OZX74_RS05940: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

OZX74_RS05945: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

Ligases: synthetase

OZX74_RS06020: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

OZX74_RS06025: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.17	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000541.5	0.15	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000542.3	0.15	ribosomal	paenicidin B	Paenibacillus terrae
BGC0000556.5	0.15	ribosomal	streptin	Streptococcus pyogenes
BGC0002294.3	0.15	other (other)	streptozotocin	Streptomyces achromogenes subsp. streptozoticus
BGC0000496.5	0.14	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001789.3	0.14	ribosomal	suicin 90-1330	Streptococcus suis
BGC0000535.4	0.14	ribosomal	nisin A	Lactococcus lactis subsp. lactis
BGC0000536.3	0.14	ribosomal	nisin Q	Lactococcus lactis
BGC0000537.3	0.14	ribosomal	nisin U	Streptococcus uberis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002323.2	0.48	ribosomal	nisin J	Staphylococcus capitis
BGC0000556.5	0.48	ribosomal	streptin	Streptococcus pyogenes
BGC0000535.4	0.48	ribosomal	nisin A	Lactococcus lactis subsp. lactis
BGC0001789.3	0.48	ribosomal	suicin 90-1330	Streptococcus suis
BGC0000536.3	0.48	ribosomal	nisin Q	Lactococcus lactis
BGC0000541.5	0.48	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000496.5	0.48	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000537.3	0.48	ribosomal	nisin U	Streptococcus uberis
BGC0000528.5	0.47	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000542.3	0.47	ribosomal	paenicidin B	Paenibacillus terrae

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for OZX74_RS05940

allorf_1411802_1411978

Leader:

MQTIKEESMSEFDLDARIEKSKPTVTPL

Core:

VTSHSLCTPGCPSGGFACFSTQCTNGCHFG

Core with dehydrations:

VDhbDhaHDhaLCysDhbPGCysPDhaGGFACysFDhaDhbQCysDhbNGCysHFG

antiSMASH-DB 4.0 matches for allorf_1411802_1411978

100.0%

NZ_CP113914.1:

Lanthipeptides: allorf_1411802_1411978_lanthipeptide

VTSHSLCTPGCPSGGFACFSTQCTNGCHFG

61.8%

NZ_KE951497.1:

Lanthipeptides: allorf_8519_8683_lanthipeptide

VTSHSLCTPGCPSGGFACFSTQCTNGCHF

+TS LCTPGCP+GG ACF+TQCTNGC

ITSKFLCTPGCPTGGIACFTTQCTNGCSI

58.8%

NZ_JALGRO010000002.1:

Lanthipeptides: MT346_RS01980_lanthipeptide

VTSHSLCTPGCPSGGFACFSTQCTNGC

+TS SLCTPGCP+GGFACF+ CT GC

ITSFSLCTPGCPTGGFACFTAACTGGC

with 113 more sequence matches

Only the first 10 are displayed here.

58.8%

NZ_SNVW01000005.1:

Lanthipeptides: EDF64_RS09155_lanthipeptide

VTSHSLCTPGCPSGGFACFSTQCTNGCHF

+TS SLCTPGCP+GGFACF+ CT GC

ITSVSLCTPGCPTGGFACFTAACTGGCTI

58.1%

NC_015600.1and 14 others:

NZ_AP020310.1 Lanthipeptides: SAGCMC97051_RS07300_lanthipeptideNZ_ATVP01000001.1 Lanthipeptides: G574_RS11150_lanthipeptideNZ_BCFB01000042.1 Lanthipeptides: GOZ36_RS07980_lanthipeptideNZ_CP068708.1 Lanthipeptides: JM964_RS04040_lanthipeptideNZ_CP116958.1 Lanthipeptides: M0P28_RS07755_lanthipeptideNZ_CP116958.1 Lanthipeptides: M0P28_RS07755_lanthipeptideNZ_FIKS01000005.1 Lanthipeptides: A0525_RS10015_lanthipeptideNZ_GL636070.1 Lanthipeptides: HMPREF9171_RS106200_lanthipeptideNZ_JADPLU010000018.1 Lanthipeptides: I4V82_RS09545_lanthipeptideNZ_JAKVIE010000017.1 Lanthipeptides: MK857_RS08340_lanthipeptideNZ_JAQDQV010000001.1 Lanthipeptides: PGO74_RS00635_lanthipeptideNZ_JAQDQV010000001.1 Lanthipeptides: PGO74_RS00635_lanthipeptideNZ_LR594050.1 Lanthipeptides: FGL20_RS03485_lanthipeptideNZ_SSXN01000004.1 Lanthipeptides: FAJ36_RS04715_lanthipeptide

Lanthipeptides: SGPB_RS10680_lanthipeptide

VTSHSLCTPGCPSGGFA-CFSTQCTNGCHFG

VTS SLCTPGC +G C T GCHFG

VTSKSLCTPGCKTGILMTCAIKTATCGCHFG

56.7%

NZ_CP024974.1and 1 other:

NZ_JAIMDQ010000006.1 Lanthipeptides: K6V44_RS04045_lanthipeptide

Lanthipeptides: CVO91_RS06555_lanthipeptide

VTSHSLCTPGCPSGGFA-CFSTQCTNGCHF

VTS SLCTPGC +G C T GCHF

VTSISLCTPGCKTGILMTCAIKTATCGCHF

56.7%

NZ_CP034587.1:

Lanthipeptides: EKH77_RS16870_lanthipeptide

VTSHSLCTPGCPSGGFACFSTQCTNGCHF

+TS SLCTPGCPS C ++ CT GCH

ITSISLCTPGCPSAA-GCTTSVCTQGCHI

56.2%

NZ_JAMDIB010000004.1:

Lanthipeptides: M5P35_RS04030_lanthipeptide

VTSHSLCT-PGCPSGGF-ACFSTQCTNGCHFG

VTS SLCT PGC +G C T GCHFG

VTSKSLCTPPGCKTGILMTCAIKTATCGCHFG

54.5%

NZ_CP085954.1:

Lanthipeptides: LK411_RS13585_lanthipeptide

VTSHSLCTPGCPSGGFACFSTQC--TNGCHF

+TS SLCTPGCP+G CF+ C TNGC

ITSKSLCTPGCPTGALGCFTQNCDPTNGCTI

53.1%

NZ_CP059471.1and 3 others:

NZ_FNZW01000001.1 Lanthipeptides: BMZ61_RS02110_lanthipeptideNZ_FOYC01000001.1 Lanthipeptides: BMZ88_RS03370_lanthipeptideNZ_KE384124.1 Lanthipeptides: G597_RS0106700_lanthipeptide

Lanthipeptides: H1R75_RS06635_lanthipeptide

VTSHSLCTPGCPSGGF-ACFSTQCTNGCHFG

+TS SLCTPGC +G C T GCHFG

ITSKSLCTPGCKTGALMTCPIKTATCGCHFG

50.0%

NZ_CP086322.1:

Lanthipeptides: K9S39_RS23570_lanthipeptide

VTSHSLCTPGCPSGGFACFSTQCTNGCHF

+TS SLCTPGC + C + +CT GCH

ITSVSLCTPGCVTAA-GCTTAKCTQGCHI

50.0%

NZ_NDXL01000003.1:

Lanthipeptides: B7024_RS31125_lanthipeptide

VTSHSLCTPGCPSGGFACFSTQCTNGCHF

+TS SLCTPGC + C ++ CT GCH

ITSWSLCTPGCVTAA-GCTTSVCTQGCHV

48.4%

NZ_GG667191.1and 1 other:

NZ_GG667191.1 Lanthipeptides: HMPREF0298_RS11685_lanthipeptide

Lanthipeptides: HMPREF0298_RS11685_lanthipeptide

VTSHSLCTPGCPSGGFACFSTQCTNGCHF

+TS SLCTPGC +G C + T GC F

ITSKSLCTPGCITGWMMCNTV--TKGCSF

MIBiG 4.0 matches for allorf_1411802_1411978

29.4%

BGC0000535.4 (ADJ56352.1):

Lanthipeptide: nisin A

VTSHSLCTPGCPSGGF

+TS SLCTPGC +G

ITSISLCTPGCKTGAL

14.7%

BGC0000535.4 (ADJ56352.1):

Lanthipeptide: nisin A

CTNGCHFG

CT GC G

CTPGCKTG

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

OZX74_RS06025: PT_FPPS_like

Domain 1 (21 - 210): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.42 out of 20.47

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 20.37 out of 27.78

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.13 out of 19.26

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 18.49 out of 20.97

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.19 out of 24.78

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.28 out of 23.05

Confidence: medium

Additional weak hits (5):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.06 out of 26.33

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 20.61 out of 31.73

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 20.26 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 20.58 out of 29.38

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.11 out of 29.65

Confidence: weak

Lanthipeptides

Terpene

TFBS Finder

Core peptide predictions

allorf_1411802_1411978 - Class I

Cleavage pHMM score: -0.00

RODEO score: 26

Monoisotopic mass: 2861.2 Da

Molecular weight: 2863.2 Da

Number of bridges: 5

Alternative weights: (assuming N unmodified Ser/Thr residues); 2881.2 Da N = 1; 2899.2 Da N = 2; 2917.2 Da N = 3

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CRP	Cyclic AMP receptor protein
CcpA	Catabolite control protein A
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
LexA variant 2	Repressor of DNA damage response
VqsM	Regulator of quorum-sensing signalling systems