LOCUS       NZ_CP120209            11098 bp    DNA     circular CON 19-MAY-2024
DEFINITION  Chryseobacterium indologenes strain Ery-6B chromosome, complete
            genome.
ACCESSION   NZ_CP120209
VERSION     NZ_CP120209.1
KEYWORDS    RefSeq.
SOURCE      Chryseobacterium indologenes
  ORGANISM  Chryseobacterium indologenes
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Weeksellaceae; Chryseobacterium group; Chryseobacterium.
REFERENCE   1  (bases 1 to 5246911)
  AUTHORS   Xu,S.
  TITLE     Synergistic degradation of erythromycin by symbiotic bacteria Ery-6A
            and Ery-6B and application in simulated water remediation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5246911)
  AUTHORS   Xu,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2023) Zhejiang Agriculture and Forestry
            University, School of Environment and Resources, East Lake Campus of
            Zhejiang A&F University, Hangzhou City, Zhejiang Province, Hangzhou
            311300, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. DEC-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: HiFiasm/Unicycler/Flye
            Genome Coverage                   :: 181.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029339075.1-RS_2024_05_19
            Annotation Date                   :: 05/19/2024 05:09:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,739
            CDSs (total)                      :: 4,629
            Genes (coding)                    :: 4,609
            CDSs (with protein)               :: 4,609
            Genes (RNA)                       :: 110
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 8 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 3 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:37:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 318027
            Orig. end                         :: 329125
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120209.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11098
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11098
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11098
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6098](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6098
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            749..1675
                     /gene="rsgA"
                     /locus_tag="PYS58_RS01470"
                     /old_locus_tag="PYS58_01470"
     CDS             749..1675
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_function="GO:0043022 - ribosome binding [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rsgA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198117.1"
                     /locus_tag="PYS58_RS01470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PYS58_01470"
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="WP_185246804.1"
                     /transl_table=11
                     /translation="MKGKIIKSTGSWYQVLELETNKIFEARIRGKFKLIKTRLTNPLAV
                     GDFVEFQLEQDDIAWITKIEPRRNYLIRKSVNLSKEAHIIASNIDLACFIFTLKHPETS
                     LGFLDRFLACCEAYNITPLILFNKIDVLHEEEIEIVKEVESDYQKIGYNTLEISSYSKL
                     NLDQLQEMLKDKTSVFFGHSGCGKSTLVNALQPGLNLKTSEISDTHLKGKHTTTFAQMH
                     FWHFGGNVIDTPGVREFAMIDIEKEEVQHYFPEIFKKREECKFHNCLHINEPKCAVIDA
                     LETGEIEHSRYATYIKLMDEAEEAAQK"
     misc_feature    2190..2207
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.76"
                     /tool="antismash"
     gene            2274..2690
                     /locus_tag="PYS58_RS01475"
                     /old_locus_tag="PYS58_01475"
     CDS             2274..2690
                     /EC_number="2.7.4.6"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013551511.1"
                     /locus_tag="PYS58_RS01475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PYS58_01475"
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="WP_034722935.1"
                     /transl_table=11
                     /translation="MSNITFTMIKPDAVADGHIGAILGKIAEGGFKIKALKLTQLTVAD
                     AKKFYEVHAERPFYGELVEFMSSGPIVAAVLEKDNAVEDFRTLIGSTNPAEAAEGTIRK
                     MFARSIGENAVHGSDSDENALIEAQFHFSGREIF"
     gene            complement(2771..4618)
                     /locus_tag="PYS58_RS01480"
                     /old_locus_tag="PYS58_01480"
     CDS             complement(2771..4618)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016199001.1"
                     /locus_tag="PYS58_RS01480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PYS58_01480"
                     /product="alpha-L-fucosidase"
                     /protein_id="WP_276284299.1"
                     /transl_table=11
                     /translation="MKNILVKPVFLGLILSAFNINAQAQTVDNSKKMEWFKNAKLGIFI
                     HWGIYSVDGISESWSFFNNYINHENYMKQLNGFSASKYQPEQWVNLIKESGAKYAVITT
                     KHHDGVSLWNSKAEKATTIPQNSLAKNDVLSPFVSVLKKSGLKTGLYFSLPDWSHPYYD
                     INTRTKKRYEIKNEPKRWQDFISYYQNQLNELSTQYAPDLLWFDGDWEHTSEEWKASQT
                     LDLLKKYNPNIIINSRLNHHGDYDTPEQGIPVVPPQNPYWELCYTMNDSWGYQPYDQKY
                     KTSNMIVRTLADVISMGGNLLLDIGPKSDGTIPEEQIEILKNLGRWTSKHQHAIYGTTR
                     GIPFDNYKGKSSLSTSKKSLFLYLDENKNFIKIYGLATKPISVKIIGDPSTVVKMDYSA
                     EKTLTLNLSNAKFDKDVTVAEVTFDNPPAFLNDFKKEKRPLSEILEMKNAQEAVYDIAN
                     ALNYDNNLVTHEGLTNDGLGMKIKKNPKTNIETLQWISKHAEALFETGKGLSEGHFPGM
                     TALSKDKQTLYLFVEGTPTGPIALKGIKNDIARIRIVGDGSMLTHSIYNKLYWSDRPGI
                     IYIDIPKERLDKQMTVIAVLLNKPVELYRENVGAIENNL"
     gene            4773..4997
                     /locus_tag="PYS58_RS01485"
                     /old_locus_tag="PYS58_01485"
     CDS             4773..4997
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PYS58_RS01485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="PYS58_01485"
                     /product="hypothetical protein"
                     /protein_id="WP_276284300.1"
                     /transl_table=11
                     /translation="MKIPALLMASLLAVGVSAQTTKPATKAKKPVKKVKKVAPAPESPE
                     KPKPTTPKAIVKKDTVKLSHFSCPACGMG"
     gene            5001..6098
                     /locus_tag="PYS58_RS01490"
                     /old_locus_tag="PYS58_01490"
     CDS             5001..6098
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /locus_tag="PYS58_RS01490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PYS58_01490"
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_185246801.1"
                     /sec_met_domain="DUF692 (E-value: 1e-21, bitscore: 75.4,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKPLLGLSMMAEPEFISAVLPLLQSNSIEVLEWSFDTFYNAQEP
                     DWLSDLLHFYSENDRLIGHGVYYSLFDAIWNERQEVWLKKLKEEFHKRKYNHITEHFGF
                     MNTENFHQGVPLPVSLHAKTLEIGKDRLYRLQDAVQVPVGIENLAFSFSLDDVTEQGIF
                     LDKLTEDTDGFLILDLHNIYCQSCNFEVEIQDIIKLYPLDKVREIHLSGGSWQESVYSK
                     SPVRRDTHDDAIPKEILSILPSVLSQCKYLEYIIVERLGHTINTEEKKIAFLDDFAQIR
                     TLIDSSDRDQQYKAPWTKKQVEFQKNPLEDKVLHEEQTLLTKLLFNKEKPESIKNHEFQ
                     YFKTENWDPEMILTAQNIIKKWNPY"
     gene            6136..6627
                     /locus_tag="PYS58_RS01495"
                     /old_locus_tag="PYS58_01495"
     CDS             6136..6627
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980025.1"
                     /locus_tag="PYS58_RS01495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PYS58_01495"
                     /product="anthrone oxygenase family protein"
                     /protein_id="WP_185246800.1"
                     /transl_table=11
                     /translation="MKMTTVLLLITAVLTALIAGLFYAYSCSVVLGLGKLSDAEYLKAM
                     QNINREILNPVFFMSFMGAAVLLPVSTFFLRGEQSVFIFLLLATLAYLIGVFGVTVVGN
                     VPMNDQLDRFDISGSTTEAIKQMRDNFENRWNFLNNIRSVFSVISITLLVCACIWHKQL
                     "
     gene            complement(6850..7500)
                     /gene="rpe"
                     /locus_tag="PYS58_RS01500"
                     /old_locus_tag="PYS58_01500"
     CDS             complement(6850..7500)
                     /EC_number="5.1.3.1"
                     /GO_function="GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="rpe"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980024.1"
                     /locus_tag="PYS58_RS01500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PYS58_01500"
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="WP_053328731.1"
                     /transl_table=11
                     /translation="MKTKLIAPSLLSADFGNLQRDIEMLNRSQADWFHIDVMDGRFVPN
                     ISFGFPVMKTVQQHAKKFVDVHLMIVEPEKYVDEFINHGADLISVHYEACTHLHRTIHH
                     IQSKGAKAGVVLNPSTPVLMLEDIIADVDLVLLMSVNPGFGGQKFIENTYKKIAETKDL
                     ILSNNSTALIEVDGGVNLDNASKLFEAGADVLVAGNAVFSAESPERTIELLKI"
     gene            complement(7838..8911)
                     /locus_tag="PYS58_RS01505"
                     /old_locus_tag="PYS58_01505"
     CDS             complement(7838..8911)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M42"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198100.1"
                     /locus_tag="PYS58_RS01505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PYS58_01505"
                     /product="M28 family peptidase"
                     /protein_id="WP_276284301.1"
                     /sec_met_domain="Peptidase_M42 (E-value: 9.7e-74, bitscore:
                     246.1, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFEKKSLKFLEKYLNTSSPTGYEHKGQEVWMDYIRPYVDKIEVD
                     HYGTCYGIINPEAEFKVVIEAHADEISWYVNYITDDGLIYVIRNGGSDQTIAPSKVVHI
                     HGENGIVKGVFGWPAIHTRTNQNEPTPKIENIFIDCGATTKKEVEEMGIFVGCMITYPD
                     EFFEMNDRYFVCRALDNRIGGFMIAEVARLLKENKKSIPFGLYITNSVQEEVGLYGADM
                     IADTIKPNIAIVTDVTHDTTTPMIEKKKEGDQKCGAGPVVFFAPSVHHTIRELIIDTAK
                     TKKIPYQRAAASRATGTDTDAFAHSNGGVPSALISLPLRYMHTTVEMVAKEDVANVIKL
                     IYETVLKIKPEMKLKYH"
     misc_feature    8917..8930
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: weak, score: 15.78"
                     /tool="antismash"
     gene            8963..9667
                     /locus_tag="PYS58_RS01510"
                     /old_locus_tag="PYS58_01510"
     CDS             8963..9667
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980021.1"
                     /locus_tag="PYS58_RS01510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PYS58_01510"
                     /product="DUF4294 domain-containing protein"
                     /protein_id="WP_185246798.1"
                     /transl_table=11
                     /translation="MNFSKIICLFMFFFGASVFGQKDTVVAKPLNQYPAESLKVDEFGN
                     KYYYDEQQKVKIYEVNGEPVVVMDELVLVNKPRFNNQLDKNYYYFLNKKLYRVYPLFVT
                     ALQQYRDIQVDMNDMDNKAKRKFVRERQNMLADQYEKQLRDLTTTEGQVFAKLMNRATG
                     KNVYEIIKELRGGFSAFWWNLKGKMADIDLKERYDPHKNRTDEFVESLLQSNWNSGYLK
                     PYPGASDFKVKK"
     gene            complement(9690..10106)
                     /locus_tag="PYS58_RS01515"
                     /old_locus_tag="PYS58_01515"
     CDS             complement(9690..10106)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846850.1"
                     /locus_tag="PYS58_RS01515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PYS58_01515"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_276284302.1"
                     /transl_table=11
                     /translation="MTKNFNIRTYACVIKEKAVLGLFERYRGKNLLKFPGGGLEYGEGL
                     IDCLHQELEEELSAKIKILEHLYTQDSLIESHFEDGNQLLSIYYIAQFTHEDNFIIKEQ
                     CIEKAEWISIEKHENPFSLPSDRIAFNKLKEKFL"
     gene            complement(10214..10630)
                     /locus_tag="PYS58_RS01520"
                     /old_locus_tag="PYS58_01520"
     CDS             complement(10214..10630)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980020.1"
                     /locus_tag="PYS58_RS01520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PYS58_01520"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_276284303.1"
                     /transl_table=11
                     /translation="MIDKINIRVYGCVVKDKKVLTLFEEYAGEPLVKFPGGGLEYGEGT
                     LECLHREFDEELNVKVDIVDHFYTQEDFLVSRFRENEQLLTIYYIVNIVDEKEFLILDP
                     CIEKTEWIDIDRPDNPFPLPIDKIVFDKLKEKFL"
     misc_feature    10855..10872
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 20.08"
                     /tool="antismash"
     misc_feature    10967..10980
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 21.64"
                     /tool="antismash"
     misc_feature    complement(11069..11083)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 24.33"
                     /tool="antismash"
ORIGIN
        1 ttttaggtca ggatattaaa aatctaggag taattcacag aggattttca acataccaaa
       61 aaacaaaata cagaaataac ccgaactggc aaattgccct tgatttcaaa agccagttcc
      121 cgaatattcc aatgctaatc gatccttctc atatttgcgg aaatagaaca ggtttggcag
      181 atattactca ggaagctctt aacgtgggct accaaggggc aattatcgaa actcactcta
      241 atccagatga agcttggagt gatgctgcac aacagatcac ccctgaagta ctggcagaac
      301 tgattggaaa cttaaaagta agaagtacaa accttgcagg gttcgaagga gaaatgggaa
      361 gacacagaac tttgatttct gatcttgatt ttcaattgat tgaacttctt tctcaaagga
      421 tgaaaatctc tgaaaaaata ggtaaactta aaaaggaaaa tgatattgca atctttcagc
      481 ctgaacgttg gaaagtaatt acagaatacg cgactcaaaa agcaaaagaa acaggaatgt
      541 ctcaggactt tattgagaaa gtattcaaag caattcatga agaatctatt gaagtacaaa
      601 acaatatcat gatcgataag aaataaaatc gaaataagag atcataagag tggggcttag
      661 ggaaaaagaa tttaggaaat cggaacattg ttttcaccta tattctcaac cctaagccct
      721 aattccttat atttgcacca tattatctat gaaaggaaaa atcattaaat ctacaggcag
      781 ttggtaccag gttttggaat tggaaacaaa taaaattttc gaggccagga tcagggggaa
      841 attcaaattg attaaaacaa gacttaccaa tccacttgcc gtaggagatt ttgtagagtt
      901 tcagctggaa caggatgata ttgcatggat tacgaagatt gagccacgca gaaattatct
      961 gatcagaaag tctgttaacc tttcaaagga agctcatatt attgcatcga atattgatct
     1021 tgcctgcttt atttttactt tgaagcaccc ggagacctca ttgggatttc tggatagatt
     1081 cctggcttgc tgtgaagcat acaatataac ccctttaatt cttttcaata aaattgatgt
     1141 tttgcatgaa gaggaaattg aaattgtaaa agaagttgag tctgattatc agaaaatagg
     1201 ctataataca ttggaaattt catcgtattc aaaactaaac ctggatcagc ttcaggaaat
     1261 gctgaaagac aaaacttctg tatttttcgg acattctgga tgtggaaagt ctacgctggt
     1321 gaatgcttta cagccgggat taaacctgaa aacttctgaa atttcggata cccatttaaa
     1381 aggaaagcat acaactactt tcgcgcaaat gcatttctgg cattttggag gaaatgttat
     1441 tgatactcct ggggttcgtg aatttgcgat gattgatatt gaaaaggaag aagtacagca
     1501 ttatttccct gaaatattca aaaaaagaga agaatgcaag ttccacaact gccttcatat
     1561 caatgagcct aaatgtgctg taatagatgc tcttgaaact ggggaaattg aacactcccg
     1621 ttatgcaacc tatataaaac tgatggatga agctgaagaa gctgcccaaa aataactata
     1681 cgaatttcta tcaataaaaa aacttcatat cataaatatg aagttttttc agttatactt
     1741 taaaaatctg ttaggtttat ttatgagtaa atatttcttt ttatcccatt tagaatctta
     1801 ggtgattttt taaagaaaaa acgattcagg atttactttt attatttttt gagattaaaa
     1861 agaatcgaat ttttagaata actctacttt ttgtcatgca tattcttgtg tataactcca
     1921 cttttctgca tcaaaatttc ccggaactaa aaaaaataaa aaaatatcaa ttatttcatt
     1981 gattgaaata gaagcaatac gatattaatc tccttttata tggtttttga ttttatcaaa
     2041 ttgttaaaat aacgccttgt tttttgcgaa taaaaaaccc agccgaagct gggtaaaaac
     2101 taataaccat gaaaactcaa attaaacatg agaatcgtaa tagaattaac aattactgtg
     2161 ccaaagattg gttcatgatt tcttaataaa agttattttt atgttaaaaa aaaattaaaa
     2221 taaaaatcaa agatattttt tgtaattttg caccattaaa aaaatataca tttatgtcta
     2281 acattacatt cactatgatt aagcctgatg ctgttgctga cggacatatc ggtgcaatat
     2341 tgggtaagat tgctgaagga ggttttaaaa tcaaagcttt aaaattaact cagcttacag
     2401 ttgctgatgc aaaaaaattc tatgaagtac acgctgaaag accattttat ggtgaattag
     2461 tagagttcat gagttctggt ccaatcgtag ctgcagtttt agaaaaagac aatgcagttg
     2521 aagatttcag aacattaatt ggttctacta accctgcaga agcagcagaa ggtacaatca
     2581 gaaaaatgtt tgctagaagc atcggagaaa atgctgttca cggttcagat tctgacgaga
     2641 atgcattaat cgaagctcag tttcattttt caggaagaga gattttctaa gagaatttct
     2701 tataaaataa aagatccgga gaattatttt ctccggatct tgtttttata tacattatta
     2761 tttccttttt ttaaaggtta ttttcaatag ctcccacatt ttccctgtac agttcaacag
     2821 gtttattaag caatactgct ataacagtca tttgcttatc cagcctttct tttgggatgt
     2881 caatgtaaat aatccccggc cggtcactcc aataaagttt attatatata ctgtgagtga
     2941 gcatacttcc atcacctaca attcttattc tggctatatc attttttatt ccttttagag
     3001 caataggacc ggtaggtgtt ccttctacaa aaagataaag tgtctgttta tcttttgata
     3061 aagcagtcat tccggggaag tgtccttctg acagtccttt tccagtttca aataaggctt
     3121 ctgcatgttt gcttatccat tgcagggttt caatgtttgt tttcggattt tttttaattt
     3181 tcataccaag accatcattg gtcagacctt cgtgtgtcac taagttattg tcatagttca
     3241 gagcattggc tatatcatac accgcttcct gagcattttt catttccagt atttcagaaa
     3301 gagggcgctt ttccttctta aaatcattca gaaaggcagg cggattatca aatgtaactt
     3361 ctgctacggt aacatctttg tcaaactttg cattggacaa gttcaaggta agtgtttttt
     3421 cagcactata atccatttta acaactgtgg atggatcacc aatgattttt actgaaatag
     3481 gttttgttgc caacccatag atctttataa agtttttgtt ctcatcaaga taaagaaaca
     3541 aagacttttt agaggtcgac aaagatgatt ttcctttgta attgtcaaaa gggattcctc
     3601 gggttgttcc atagatggca tgctgatgtt tggaagtcca gcgtccaaga tttttaagaa
     3661 tctcaatctg ttcctcagga atggtaccat ctgatttcgg accgatatca agtaaaagat
     3721 ttcctcccat actgatcaca tctgccagag ttcttacaat catattggat gttttatact
     3781 tttgatcata cggctggtat ccccaggaat cattcatggt ataacaaagt tcccagtatg
     3841 gattttgtgg aggaactacc ggaataccct gttcaggggt atcataatcc ccgtggtgat
     3901 tgagtcttga gttgatgata atattaggat tgtatttctt gagtaaatcc agtgtttgag
     3961 aagctttcca ttcttcagaa gtatgttccc aatctccatc gaaccaaaga agatccggag
     4021 cgtattgagt ggaaagttca ttaagttggt tttgatagta gctgataaaa tcctgccatc
     4081 gtttaggctc attttttatt tcataacgtt tttttgtccg ggtattgata tcataatagg
     4141 gatgactcca atccggtaat gagaaataaa gacctgtttt taatcccgat tttttcaaca
     4201 cagaaacaaa aggacttaaa acatcgtttt ttgccagaga attttgagga atagttgttg
     4261 ctttttctgc tttggagttc cagagcgaaa ctccgtcatg atgttttgtt gtaatcacag
     4321 catattttgc tccggattcc ttaataagat ttacccattg ctctggctgg tattttgaag
     4381 ctgaaaaacc attcagctgt ttcatgtaat tttcgtgatt aatataatta ttgaaaaatg
     4441 accaggattc agagatgcca tccacagagt agattcccca atgaataaaa atgcccagtt
     4501 ttgcattttt aaaccattcc atttttttac tgttgtcaac tgtttgagcc tgggcattga
     4561 tattgaatgc agataatatc aatccaagaa aaacaggttt aaccagaatg tttttcatat
     4621 atagatttat tttaacatta aatataaata aacaaaatag catagtataa aatagttttt
     4681 gatatttact agtaattgtt attaaaacgt ttgttattat tgtattttta taaatggaac
     4741 atttattgta aaaactcttc taaaaaaaca ttatgaaaat tccagcatta ttaatggcaa
     4801 gtttattagc ggtaggagtt tctgcacaaa ctacaaaacc tgctacaaaa gctaaaaagc
     4861 ctgtaaagaa agttaaaaaa gtagctccgg ctcctgaaag tccggaaaag cccaagccaa
     4921 ctactcctaa agccattgtt aaaaaagata cagttaaact tagtcatttt tcatgtccag
     4981 cctgtggaat gggatagagt atgaaaaagc cgctgttagg attatccatg atggcagaac
     5041 ctgagtttat ttctgctgtt ttacctttat tacaaagcaa ttctattgaa gtgcttgaat
     5101 ggtcatttga tacattctac aatgcgcaag aaccggattg gttaagtgat ctgcttcatt
     5161 tttattcaga aaatgatcga ttgattggac atggcgtata ttattcattg tttgatgcta
     5221 tatggaacga aaggcaggag gtatggctta aaaaattaaa agaagaattt cataaaagaa
     5281 aatataatca cattaccgaa catttcggtt ttatgaatac agaaaacttt catcagggag
     5341 tacctttacc agtctctttg cacgctaaaa ccttagagat cggtaaagat cggctttaca
     5401 ggctgcaaga cgctgtacag gttcctgttg gaatagaaaa tcttgccttt tctttttcat
     5461 tggatgatgt gactgaacag ggaatatttc tcgataaact tacagaggat acggatggat
     5521 tccttattct tgatctacat aatatttatt gccagtcctg taattttgag gtggaaatac
     5581 aggatattat taaactatat cctttggata aagtcagaga aattcatctt tcaggaggaa
     5641 gctggcagga aagtgtatac agtaagagcc cggtaaggag agatactcat gatgatgcga
     5701 tccctaaaga aattttatct atactgcctt cagtcttatc tcaatgtaaa tatttggaat
     5761 atattatcgt cgaaaggtta gggcacacca ttaatacaga agaaaaaaag atagcttttc
     5821 tggatgattt tgcacaaata agaacgttga ttgattcatc agaccgggat cagcaatata
     5881 aagcaccctg gactaaaaaa caggtagaat ttcagaaaaa tcctttggaa gataaagttc
     5941 ttcacgaaga acaaacattg ctgacaaaac tgctatttaa taaggaaaaa cctgaatcta
     6001 ttaaaaatca tgaattccaa tattttaaaa cagaaaactg ggatccggag atgattctta
     6061 ctgctcagaa tatcattaaa aagtggaacc cttattaagt aacgatgttc ctctagtaac
     6121 cacaaaccaa attatatgaa aatgacaacc gtattattac tcataacagc tgtgcttaca
     6181 gcacttatag ccggactttt ctatgcttat tcgtgttcag tagtactggg attaggtaaa
     6241 ttatctgatg ctgaatatct gaaggccatg cagaatatta atcgagaaat actgaatcct
     6301 gtctttttta tgagtttcat gggggctgcc gttcttcttc ctgtgtctac tttttttcta
     6361 cggggagaac aatctgtctt tatttttctc ttattagcca cattggctta tctgataggt
     6421 gttttcgggg ttacggtagt aggaaatgtc cctatgaatg atcaacttga tagatttgat
     6481 atctcaggct ctacaactga agctataaag cagatgcgag ataatttcga aaacagatgg
     6541 aattttctga acaatataag atctgttttt tcagtaatca gtataacgtt actggtttgt
     6601 gcctgtattt ggcataagca actatagaca gtttttttta caataggtaa aagtacttgg
     6661 atttaaattc cgtatttcta cggattgact ggcgttcatt ttatgcagaa ctttgtgttg
     6721 ttagtaagaa ggaatttaga catagaaaca ttttgaaaaa aaattaataa ccaggacgac
     6781 tcaaattcaa attgaaaaag gcagttccat tggaactgcc ttttgccatc tatttaaact
     6841 tgtatctttt tagattttta aaagttcaat ggttctttcc gggctttcag cagaaaatac
     6901 tgcatttccg gcaaccaaaa catccgctcc ggcttcgaaa agcttagagg cattgtcaag
     6961 attcactccg ccatctactt caataagtgc tgtagagtta ttgcttaaga taaggtcttt
     7021 tgtttcagca atctttttat aagtattttc aatgaatttc tggcctccaa atcctggatt
     7081 cacactcatt aataatacaa gatctacatc tgcaataata tcttcaagca ttaatacagg
     7141 tgtagatgga ttcaaaacaa cacctgcttt tgctccttta ctctgaatgt ggtgaatggt
     7201 tctgtgaaga tgagtacatg cttcataatg tacagaaata aggtcagcac catggttgat
     7261 gaattcatca acatattttt caggctccac aatcattaaa tgaacgtcca cgaatttttt
     7321 agcatgctgc tgaacggttt tcatcaccgg aaaaccaaat gaaatgttag gaacaaatct
     7381 gccgtccatt acatcaatgt ggaaccaatc tgcctgagat ctgttcagca tttcaatatc
     7441 tctttgcaga ttcccaaagt ctgcggataa aagggatgga gcaataagct tcgttttcat
     7501 ttttactttt tatactttta cttagatttc tctctgttat gagggattaa caactaaaaa
     7561 ataatataag gaaatacttc caagagtaat tatttctatg attgttaatt taatgttgtt
     7621 aatttttaaa accaacaatc gatctattat aaataatcct aaaggtacaa tactgacaat
     7681 taataacatt tcaagaatca tattgtatgg tgatccggtt ttgggtggat cgaacaactt
     7741 atatatactt aaaaatgcta tataactaaa aaaaagagca cttaaaatgg atataaacgt
     7801 tggttttcgg actaaatgat aaaagacttc tttcatttta atgatacttc agtttcattt
     7861 ccggtttaat ctttaaaacc gtttcgtaga tcaatttaat gacattcgct acgtcttctt
     7921 tagccaccat ttctaccgtt gtatgcatat agcgcaaagg taatgaaatt aaagcacttg
     7981 gtacaccgcc attagaatga gcaaaagcat ctgtgtctgt tccagttgct ctgcttgctg
     8041 cggctctctg ataagggatt ttctttgttt ttgcagtgtc aataatcaat tctctgatgg
     8101 tatgatgaac actcggagcg aagaatacta ccggtccggc accacatttt tggtctcctt
     8161 ctttcttttt ttcgatcatt ggagtggtgg tatcgtgcgt tacatctgtt acaattgcaa
     8221 tattaggttt gatggtatca gcaatcatat ctgctccata taaccctact tcttcctgta
     8281 cggaattggt aatataaaga ccaaatggaa tagatttttt gttttctttt aaaagtcttg
     8341 ccacttcagc aatcatgaaa cctccgattc tgttgtcaag agctctgcag acaaaatatc
     8401 tgtcgttcat ctcaaagaac tcatcagggt aagtaatcat gcatcctaca aatattccca
     8461 tttcttcaac ttcttttttg gtagtagcac cacagtcgat aaagatgttt tcaatttttg
     8521 gagtaggttc attctgattg gttcttgtat ggatagccgg ccatccgaat actcctttta
     8581 caattccgtt ttctccatga atatgcacta cttttgatgg agcaatagtt tggtctgaac
     8641 ctccgtttct gattacataa atcaatccat catctgtaat atagtttaca taccacgaga
     8701 tttcatcagc atgagcttca atcactactt taaattcagc ttccggattg atgatcccat
     8761 agcatgttcc gtaatgatcc acttcaatct tgtctacata aggtctgatg tagtccatcc
     8821 aaacttcctg tcctttgtgt tcgtatcctg ttggagatga agtgtttaaa tatttctcta
     8881 aaaatttcaa agatttcttt tcaaatttca taaaaaggaa tgatttttgc gtttaatttt
     8941 tgttcttata agtgtaaaaa taatgaattt tagtaagatt atctgtcttt ttatgttctt
     9001 ttttggagcc agcgtttttg ggcagaagga tactgtggtg gcaaagccac tgaatcaata
     9061 ccccgctgaa tctttaaaag tagatgaatt tggcaataaa tattattacg acgaacagca
     9121 gaaggttaag atctatgaag taaacggaga gcctgtggta gtaatggatg agttggtttt
     9181 agtgaataag ccgaggttta ataatcagtt ggataaaaat tactattatt tcttaaataa
     9241 gaagttgtac agagtatatc cgttatttgt aaccgcttta cagcaataca gagatattca
     9301 ggtagacatg aatgatatgg ataataaagc caagagaaag tttgtaagag aaaggcaaaa
     9361 tatgcttgct gatcaatatg aaaaacaatt gagagatctt accactaccg aaggccaggt
     9421 ttttgcaaaa ctgatgaaca gagcaaccgg gaaaaatgta tatgaaatta ttaaagagct
     9481 aagaggtggt tttagtgcct tttggtggaa tcttaaaggg aaaatggctg atattgatct
     9541 gaaagagcga tatgatcccc acaaaaacag gactgatgaa tttgtggaat cattactcca
     9601 gtctaattgg aattcagggt atttgaaacc ttatccagga gccagtgatt ttaaagtaaa
     9661 gaaataatat aaaattcctg taagtgagct tacaggaatt tttcttttaa tttattaaac
     9721 gcaatccgat cagaaggtag tgaaaaagga ttctcatgct tttcaataga gatccattct
     9781 gctttttcaa tgcattgttc tttgattata aaattgtctt catgggtgaa ttgggcaata
     9841 taatagatag ataaaagttg atttccatct tcaaagtgtg attcaataag agagtcctga
     9901 gtatagaggt gttccagtat tttgattttt gcactcaatt cttcctccaa ttcctggtgc
     9961 aggcagtcta ttaatccttc tccatattcc aatcccccgc caggaaactt taataagttt
    10021 tttcccctat atctttcaaa aagaccaaga actgctttct cctttataac acaagcatag
    10081 gttcttatgt taaaattttt agtcatgatt attatttgat agtgtgaatt tagttaaatt
    10141 ttatcacatt atgttgaata atactagaaa atgaaaaaaa gaatatggac taacaataaa
    10201 aaagtttctc tatttagaga aacttttctt ttaatttatc gaatacaatt ttatctatag
    10261 gaagtgggaa agggttatcc ggtctgtcga tgtctatcca ttccgtcttt tcaatacagg
    10321 gatctaaaat aaggaactcc ttctcatcaa caatattgac tatataatat atggtaagaa
    10381 gttgttcgtt ttctctgaaa cgggaaacaa gaaagtcttc ttgagtatag aaatggtcta
    10441 caatatccac tttcacattc aattcctcat cgaattcacg gtgcagacac tctaatgttc
    10501 cttcgccata ttccaatccg ccacccggaa atttcactaa aggttcgccg gcatattctt
    10561 caaataaagt gagtactttt ttatctttta ccacacagcc atacactcta atgttgatct
    10621 tatctatcat atatataatg tataatattt ctatagctaa ggtaatgaat ttttgtgagg
    10681 cgtatagtgg taaaaaaggc aaaataccga aaaaagtaaa tggccttaag ggttctatta
    10741 tttatagctt tatggcatta atcatttctc ttttccctgg tggaccctgt tttttctcta
    10801 cgtgaaagtt gagctcctga aggattcgtc tcacgcttcc tttagaagaa taggttgtta
    10861 ataatccgtt aatagccatt ttgtcagcta ccagttcaaa taacggtttt tcccaaagat
    10921 ccggctgcac tcttgctcca aaacagtcga aataaacaag gtttatttta ggcagatcaa
    10981 tgtgtttaag gtcaaaaaag tcacattcta tctttttaag gttgaatcca ctaatgattt
    11041 ctactgactt ttcccaatct gctagatgaa ttttctgata aatattttta aactctgg
//
