LOCUS       NZ_CP119144            10216 bp    DNA     linear   CON 22-NOV-2023
DEFINITION  Streptomyces noursei strain C27 chromosome, complete genome.
ACCESSION   NZ_CP119144
VERSION     NZ_CP119144.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces noursei
  ORGANISM  Streptomyces noursei
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9627936)
  AUTHORS   Go,B. and Lu,Y.
  TITLE     Control effect and mechanism of Streptomyces NK27 against gray mold
            of grape
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9627936)
  AUTHORS   Go,B. and Lu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-FEB-2023) State Key Laboratory for Biology of Plant
            Diseases and Insect Pests, Institute of Plant Protection, Chinese
            Academy of Agricultural Sciences, Yuanmingyuan, Beijing 100193,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-OCT-2021
            Assembly Method                   :: ABySS v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 98x
            Sequencing Technology             :: Complete Genomics
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 14:00:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,442
            CDSs (total)                      :: 8,348
            Genes (coding)                    :: 8,203
            CDSs (with protein)               :: 8,203
            Genes (RNA)                       :: 94
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 46 of 145
            Pseudo Genes (incomplete)         :: 121 of 145
            Pseudo Genes (internal stop)      :: 12 of 145
            Pseudo Genes (multiple problems)  :: 30 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:17:19
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7636723
            Orig. end                         :: 7646939
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP119144.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="28"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5216
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(707..721)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.29"
                     /tool="antismash"
     gene            1064..2257
                     /locus_tag="P0D74_RS33500"
     CDS             1064..2257
                     /EC_number="2.3.1.29"
                     /GO_function="GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (45-388). E-value:
                     8.1e-72. Score: 234.2. Matches aSDomain:
                     nrpspksdomains_P0D74_RS33500_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1109: 8-amino-7-oxononanoate synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016577364.1"
                     /locus_tag="P0D74_RS33500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycine C-acetyltransferase"
                     /protein_id="WP_275465911.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.2e-69, bitscore:
                     234.1, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFDAVRDDLRTALDEIRAAGLHKPERVIGTPQSATVAVTGGGRPG
                     EVLNFCANNYLGLADHPEVIAAAHGALDRWGYGLASVRFICGTQEVHKELEQRISHFLG
                     QEDTILYSSCFDANGGVFETLLGPEDAVISDALNHASIIDGIRLSKARRLRYANRDLAD
                     LERQLKEAQGARRTLVVTDGVFSMDGYVAPLREICDLADRYGAMVMVDDSHAVGFVGAG
                     GRGTPELCGVMDRVDIITGTLGKALGGASGGYVAARAEIVALLRQRSRPYLFSNSLAPV
                     IAAASLKVLDLLEAADDLRERLHANTALFRSRMIEEGFAILPGAHPIAPVMIGDAAEAA
                     RMAELLLERGVYVIGFSYPVVPQGEARIRVQLSAAHSTEDVHRAVDAFVAARAALGS"
     aSDomain        1199..2227
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_P0D74_RS33500_Aminotran_1_2.1"
                     /evalue="8.10E-72"
                     /label="P0D74_RS33500_Aminotran_1_2.1"
                     /locus_tag="P0D74_RS33500"
                     /protein_end="388"
                     /protein_start="45"
                     /score="234.2"
                     /tool="antismash"
                     /translation="EVLNFCANNYLGLADHPEVIAAAHGALDRWGYGLASVRFICGTQE
                     VHKELEQRISHFLGQEDTILYSSCFDANGGVFETLLGPEDAVISDALNHASIIDGIRLS
                     KARRLRYANRDLADLERQLKEAQGARRTLVVTDGVFSMDGYVAPLREICDLADRYGAMV
                     MVDDSHAVGFVGAGGRGTPELCGVMDRVDIITGTLGKALGGASGGYVAARAEIVALLRQ
                     RSRPYLFSNSLAPVIAAASLKVLDLLEAADDLRERLHANTALFRSRMIEEGFAILPGAH
                     PIAPVMIGDAAEAARMAELLLERGVYVIGFSYPVVPQGEARIRVQLSAAHSTEDVHRAV
                     DAF"
     gene            2315..3223
                     /locus_tag="P0D74_RS33505"
     CDS             2315..3223
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494042.1"
                     /locus_tag="P0D74_RS33505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_020930629.1"
                     /transl_table=11
                     /translation="MIEARRLHILRALADHRTVTAAAAALYLTPSAVSQQLAALEQETG
                     HRLVERGARGVRLTAAGDILLGHANAVLAQLERAEAELAAYDEGEAGTVTVAAFATGIA
                     LVLAPALAELAGSAPGIRVRVQDAEGDASLLMVLDRQVDVAVAVEYRGAPDADDPRLTR
                     VPLYAEPFDAVLPPGHPLAGVAAVPLAGLAKDPWIGPYSGNPCHDVVVLACEYAGFRPR
                     LTHSSDDFRAVVALASAGVGVALVPRSALRGMDLGGVVVRPVDGVAPTRRVFAAVRRGA
                     EDHPLFRPVLTALRATAADLG"
     misc_feature    2334..2350
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 21.67"
                     /tool="antismash"
     gene            complement(3287..4327)
                     /locus_tag="P0D74_RS33510"
     CDS             complement(3287..4327)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676609.1"
                     /locus_tag="P0D74_RS33510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PHP domain-containing protein"
                     /protein_id="WP_275465912.1"
                     /transl_table=11
                     /translation="MDPVAALERIAFLLERQAAVTYRVQAFRTAASVLTALPADALRER
                     AGNGTLGRLKGLGPKTTRVVEEALAGERPAYLTRLEEEAGGPLVEGEAGRALRRALRGD
                     CHLHSDWSDGGSPVEEMARTARDLGHAWCALTDHSPRLTVARGLTAERLRQQLAVVAEV
                     NARLAPFRLLTGIECDILDDGSLDQEPELLDELDVVVASVHSKLRMDAGPMTRRLLAAV
                     RNPLVDVLGHCTGRQLTGKLRPESEFDATEVFAACAEHRTAVEINCRPDRLDPPRRLLR
                     HALDAGAFFAVDSDAHAPGQLDWQIYGCARAEECGVPTDRIINTWMARQLTAWKRTGRP
                     PRSAGT"
     misc_feature    4505..4518
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.35"
                     /tool="antismash"
     misc_feature    complement(4505..4521)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.68"
                     /tool="antismash"
     misc_feature    complement(4506..4519)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.36"
                     /tool="antismash"
     misc_feature    complement(4507..4520)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.23"
                     /tool="antismash"
     misc_feature    complement(4508..4521)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.83"
                     /tool="antismash"
     misc_feature    4510..4526
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.4"
                     /tool="antismash"
     misc_feature    complement(4513..4530)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.91"
                     /tool="antismash"
     misc_feature    complement(4600..4609)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    4614..4632
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 18.26"
                     /tool="antismash"
     misc_feature    complement(4968..4981)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.77"
                     /tool="antismash"
     misc_feature    complement(4968..4982)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.89"
                     /tool="antismash"
     misc_feature    complement(4969..4982)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.98"
                     /tool="antismash"
     misc_feature    4969..4983
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.56"
                     /tool="antismash"
     gene            5001..5216
                     /locus_tag="P0D74_RS33515"
     CDS             5001..5216
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576071.1"
                     /locus_tag="P0D74_RS33515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type A2 lantipeptide"
                     /protein_id="WP_275465913.1"
                     /sec_met_domain="strepbact (E-value: 5.2e-23, bitscore:
                     80.3, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNAQVQTQEISDADLDNVSGGLVGGVVNTATTTVGSVAPVTGVL
                     NTATGLVQQTTGLDVQGVAGNLTAGL"
     gene            5450..6256
                     /locus_tag="P0D74_RS33520"
     CDS             5450..6256
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029: RND family
                     efflux transporter MFP subunit"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540972.1"
                     /locus_tag="P0D74_RS33520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_275465914.1"
                     /transl_table=11
                     /translation="MQFRQQALSKLQSPEELDLPVRFARPQGWLVLTVTVLVMIAAAVW
                     AVTGTVSATVSAPGILTHGHGSYVLQSPVAGQVTEVLVEEGKQVTAGAPLVRIRTAQGT
                     TTVVRALAAGRLTNLVATIGAVVTTGADLASVERTTGTRDPLTATLYVPAENAATLPVG
                     APVDLTVAAAPTQRFGVLRGHVAAVGRAAQSRQRISAFLGSTQLGEQFSRAGQPIAVLV
                     RLDPARTASGYAWSTSDGPPFALDSMTQATAAVHLAAQHPIDWLLS"
     misc_feature    complement(5461..5478)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.48"
                     /tool="antismash"
     misc_feature    complement(5473..5485)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 16.37"
                     /tool="antismash"
     gene            6253..8532
                     /locus_tag="P0D74_RS33525"
     CDS             6253..8532
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540973.1"
                     /locus_tag="P0D74_RS33525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_275465915.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 6.2e-37, bitscore:
                     125.3, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAPDTSAAPPQHLPPAGRGRHRPEGAPPARRRRPPAAAPKAKKA
                     VRTPTVLQMEAVECGAASLAMVLAHYGRHIPLEELRIACGVSRDGSRASNLLKAARSYG
                     LSAKGMQMEPAALADVAAPAILFWEFNHYVVYDGPGRRLGRRGVHINDPDKGRRFVPVE
                     DFDTSFTGVVLVFEPTEAFRTGGRRPSVLSALPARLRGTTGTLLAALLASLLLVGVGAA
                     LPSLSRTYIDMFLIGNQTSLLGPLFAAMAAMVVLTAVLTGLQQANLLRGRIISSTLSSA
                     RFLRHLLRLPVTFFAQRSPADLVQRLQSNDAVAETLARDLAAAAVDGVVVVLYAVLLWT
                     YDPQLTVIGVLIALLNVVAMRIVIRLRATHTQKLRADTARLTNTSYTGLQLIETMKATG
                     GENGYFRRWAGQHAATLEEQQRLGVPSAWLAVVAPTLATLNSALILWIGGLRAVEGHIS
                     IGLLVAFQALVTRFTAPITRLNGVAGRIQDFAADVARLKDVESFPADAHYSRPDADAGT
                     RRLKGHVTLEDVTFGYSPLDAPLLTGFSLAVGPGQQVALVGGSGSGKSTVSRLISGLYA
                     PWEGTIRIDGQRLADIPRSALAASVSFVDQDVFLFEGTVRDNVALWDPSIPDDAVIAAL
                     QDAALYDDVIARRPDGIHSRVEQDGRNFSGGQRQRLEIARALVRRPSILVLDEVTSALD
                     AGTEAVIMDNLRRRGCACVIIAHRLSTVRDSDEIVVLDHGAVVERGRHAELVAAGGAYA
                     ELVKEH"
     misc_feature    complement(9919..9928)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
ORIGIN
        1 tctggctgat ggacgtgccg gagccggagg tcggcccggg cgacgtcctg atcaaggtgt
       61 tgcgcaccgg catctgcggc accgatctgc acatccgcgc ctgggacggc tgggcgcagc
      121 aggctgtcag cgccccgctg acggtcggcc acgagttcgt cggggaggtc gtcgggaccg
      181 gccgggacgt ggccgacatc aaggccggtg acctggtcag tggcgagggc cacctggtct
      241 gcggcagctg ccgcaactgc caggccggcc gccgccacct gtgccgcgcc acggtgggcc
      301 tgggtgtcgg acgggacggc gcgttcgccg agtacgtcgc gctgcccgcc gccaacgtgt
      361 gggtgcaccg ggtccccgtc gacctcgaca tcgccgcggt cttcgacccg ttcggcaacg
      421 ccgtgcacac cgcgctgtcc ttcccgctgg tcggcgagga cgtgctgatc accggcgccg
      481 ggccgatcgg catcatggcg gcggcggtcg cccggcacgc cggcgcccgc aacgtggtga
      541 tcaccgacgt cagcgagcag cgcctggacc tggcccgcaa ggtcggcgtc agcctcgccc
      601 tcaacgtcgc cgccacctcg atcgaggccg gccagcgcga actgggcctg cgcgagggct
      661 tcgacgtcgg cctggaaatg tccggccgcc ccgaggcgct gcgcggcatg atcggaaaca
      721 tgacgcacgg cggtcggatc gccatcctgg ggctgccgtc cgaggagttc ccggtcgact
      781 ggtcccggat cgtcacctcc atgctcacga tcaagggcat ctacggccgg gagatgttcg
      841 agacctggta cgcgatgtcc gtgctgctgg agggcggcct cgacctcgcg cccgtgatca
      901 ccggccgcta cccgtaccag gacttcgacg ccgccttcgc cgacgcggcg agcggccagg
      961 gcggcaaggt catcctcgac tggagcgcct gaccccgccc gcccctcccc aagacgccac
     1021 cgcccgccgg cccgacccgc gcacccccaa ggagacaccc ctcatgttcg acgccgtacg
     1081 cgacgacctg cgcaccgcac tcgacgagat ccgggccgcc ggcctgcaca agccggagcg
     1141 ggtgatcggc accccgcagt ccgccaccgt cgcggtcacc ggcggcggcc gccccggcga
     1201 ggtcctcaac ttctgcgcca acaactacct gggcctggcc gaccaccccg aggtcatcgc
     1261 cgccgcccac ggggccctcg accgctgggg ctacgggctg gcgtcggtcc gcttcatctg
     1321 cggcacgcag gaggtgcaca aggagctgga gcagcggatc tcccacttcc tcggccagga
     1381 ggacacgatc ctgtactcct cctgcttcga cgccaacggc ggcgtcttcg agaccctgct
     1441 cggcccggag gacgcggtga tctccgacgc cctcaaccac gcctccatca tcgacggcat
     1501 ccggctctcc aaggcccgcc gcctgcgcta cgccaaccgc gacctggccg acctggagcg
     1561 gcagttgaag gaggcgcagg gggcccggcg caccctggtc gtcaccgacg gcgtgttctc
     1621 catggacggc tacgtggcgc cgctgcggga gatctgcgac ctggccgacc gctacggcgc
     1681 gatggtcatg gtcgacgact cgcacgcggt cgggttcgtc ggggccggtg gccgcggcac
     1741 cccggagctg tgcggggtga tggaccgggt cgacatcatc accggcacgc tcggcaaggc
     1801 gctgggcggt gcctccggcg gatacgtcgc ggcgcgcgcc gaaatcgtcg cgctgctccg
     1861 ccagcgctcc cgcccctacc tcttctccaa ctcgctcgcg ccggtgatcg cggccgcctc
     1921 gctcaaggtc ctggacctcc tggaagccgc cgacgacctg cgcgaacgcc tgcacgccaa
     1981 caccgcgctg ttccgttcgc ggatgatcga ggagggcttc gcgatcctgc ccggcgcgca
     2041 cccgatcgcc cccgtgatga tcggggacgc ggccgaggcg gcccggatgg ccgaactgct
     2101 gttggagcgc ggggtgtacg tcatcggctt ctcgtacccg gtggtgcccc agggcgaggc
     2161 ccggatccgc gtccagctgt cggccgccca ctccaccgag gacgtgcacc gcgcggtgga
     2221 cgccttcgtg gcggcccgcg cggcgctggg gagctgaccg gcccgaccgg gggcggccgg
     2281 gcacgatccg gccgaaccct gcgaaactgg acccatgatc gaagcacgtc ggctgcacat
     2341 cctccgtgcg ctggccgacc accgcacggt gaccgccgcg gccgccgcgc tgtacctcac
     2401 cccctcggcg gtctcccagc agctcgccgc gctggaacag gagaccggcc accgcctcgt
     2461 ggagcgcggc gcccgcggcg tccggctgac cgccgcgggc gacatcctgc tcggccacgc
     2521 caacgccgtc ctcgcccagc tggagcgggc cgaggcggag ctggccgcgt acgacgaggg
     2581 cgaggcgggc accgtgacgg tggccgcgtt cgcgaccggc atcgcgctgg tgctcgcccc
     2641 ggccctcgcc gaactggccg gttccgcgcc cggcatccgc gtccgggtcc aggacgcgga
     2701 gggcgacgcc agcctgctga tggtgctgga ccggcaggtc gacgtggcgg tggccgtcga
     2761 gtaccggggc gcgccggacg cggacgaccc tcgtctcacc cgcgtcccgc tctacgccga
     2821 accgttcgac gcggtgctgc cgccggggca cccgttggcc ggggtggcgg cggtgccgtt
     2881 ggccgggctc gccaaggacc cgtggatcgg cccctattcc ggcaatccgt gtcatgacgt
     2941 ggtcgtgctg gcctgcgagt acgccggctt ccggccgcgc ctgacgcact cctcggacga
     3001 cttccgggcg gtggtggcgc tggcctcggc gggggtgggg gtggccctgg tgccccgctc
     3061 ggcgctgcgc gggatggacc tcgggggcgt cgtggtgcgg ccggtcgacg gggtggcgcc
     3121 cacccggcgg gtcttcgccg cggtccggcg gggcgcggag gaccacccgc tgttccgccc
     3181 ggtgctgacg gccctgcggg cgacggcggc cgacctgggg tgagccgccg gggcggtggg
     3241 cggccgccgc cgggccgacc gcgcgccggg gcgcgcccgg cggccgtcag gtgccggcgc
     3301 tgcgcggcgg ccggccggtc cgcttccagg ccgtcagttg acgggccatc caggtgttga
     3361 tgatccggtc ggtgggaacg ccgcactcct cggcgcgggc gcagccgtag atctgccagt
     3421 ccaactggcc gggggcgtgc gcgtcgctgt cgacggcgaa gaaggcgccg gcgtccaggg
     3481 cgtggcggag caggcggcgc ggcgggtcga ggcggtccgg gcggcagttg atctccaccg
     3541 cggtgcggtg ctcggcgcac gccgcgaaga cctccgtggc gtcgaactcc gactccgggc
     3601 gcagcttgcc ggtgagctgc cgcccggtgc agtggccgag cacgtccacc aacgggttgc
     3661 ggaccgcggc cagcagccgc cgggtcatcg gcccggcgtc catccgcagt ttcgagtgca
     3721 ccgaggcgac cacgacgtcg agctcgtcca ggagttccgg ttcctggtcg agggagccgt
     3781 cgtcgaggat gtcgcactcg atcccggtca gcagccggaa cggcgccaac cgggcattga
     3841 cctcggccac cacggccaac tgctgccgca gccgctcggc ggtcagtccg cgggccacgg
     3901 tgagccgggg ggagtggtcg gtcagcgcgc accaggcgtg gccgaggtcg cgggcggtgc
     3961 gggccatctc ctcgacgggg ctgccaccgt ccgaccagtc cgagtgcagg tggcagtcgc
     4021 cgcgcagggc ccggcgcagg gcccgacccg cctccccctc gaccaacggg ccgccggcct
     4081 cttcctccag gcgcgtcagg taggcgggcc gctccccggc gagcgcctcc tcgacgaccc
     4141 gggtcgtctt ggggcccagt cccttcagcc gccccagcgt tccgttgccg gcccgctcgc
     4201 gcagggcgtc cgcgggcagt gcggtcagca ccgacgccgc ggtgcggaac gcctgtaccc
     4261 ggtacgtcac cgctgcctgt cgctccaaca ggaacgcgat ccgctccagc gccgctacgg
     4321 gatccactta cccaccgtgc accgggcggg gggtgcctgc caccgcggcc ggcccggcga
     4381 acggtgccca ccgcagttat gcgaatatgt ccgttctctg gatgctcgat ccgtttccgt
     4441 cggaatgacg gggcgatagc cgtgatgatt ccggtcatta gtgacgtgga agtcgagggc
     4501 aatcatgttt ggttaaaacc tgtgcaacat ccaggtcgcg tggcggaaac gcaaagcgga
     4561 cttcgcgtcg tcgcggggtg ctggtcgggc gttaagtcgg gccgccttca ggaaatctgc
     4621 acaccgaatg cgcagccggt acacgggccg cggtttcgcg tgtgtgcagc gagggcccgc
     4681 tcggcgactt gcatccggaa tgtcctattc cgcagggctg gaagcgagag ttcggcggaa
     4741 aggaaaccgg agaagatgtg aagatgcttc gaccatgcca tggacgcgca cacacgaccg
     4801 gcaatttcgc ggggtggata cgtccggatc gccgctcatc cgattagcag cgcaacccgc
     4861 ctacatgctt tgatccatcg caccgcggga gtcaccgggg tttcccgata tcggggcccg
     4921 gactaccgtg gccgcggccg gggcacccac cggacgaccc gtacgcggtt tttcttgata
     4981 ttgatccgaa gggaaacatc atgaacaacg cccaggtgca gacccaggaa atttccgacg
     5041 ccgacctgga caacgtgtcc ggtggcctcg tcggcggtgt cgtgaacacc gccaccacca
     5101 ccgtcggctc cgtcgccccg gtcaccggtg tcctcaacac cgccaccggc ctggtccagc
     5161 agaccaccgg cctggacgtc cagggcgttg cgggtaacct gaccgccggt ctctgacccg
     5221 gagccgaccc tgtgccgccg cgcggcacag ccggcatcca acggtcgcga cggcaatcgg
     5281 cgccccctcc ctgccgatcc gccgtgtcga gtgccccgga caacccggtt ccgaccgggt
     5341 tccggggcac ccggctgtcc gcgaccggaa ggcgccggag ttggaaccgc gcgcccccgg
     5401 cgcgcgtctg tacccccgta tcagccgctg cagttgaggg aagagtgtcg tgcagttccg
     5461 tcaacaggcc ctttccaagc tgcaatcgcc ggaggaactg gacctgccgg tgcgcttcgc
     5521 ccgcccgcag ggctggctgg tgctgaccgt caccgtcctt gtcatgatcg ccgccgccgt
     5581 ctgggctgtc accggcaccg tctccgccac cgtcagcgcc cccggcatcc tcacccacgg
     5641 ccacggcagt tacgtgctcc agagcccggt cgccggacag gtcaccgagg tcctcgtcga
     5701 ggagggcaaa caggtcaccg ccggcgcgcc gctggtgcgg atacgcaccg cccaaggcac
     5761 caccaccgtc gtccgggcgc tcgccgccgg ccggttgacc aatctcgtcg ccaccatcgg
     5821 cgcggtcgtc accaccggcg ccgacctcgc ctcggtggaa cgcaccaccg gcacccgcga
     5881 cccgctcacc gccacgctct acgtcccggc cgagaacgcc gccacccttc cggtcggcgc
     5941 ccctgtcgac ctcaccgtcg ccgccgcccc cacccagcgg ttcggggtac tgcgcggcca
     6001 tgtcgcggcc gtcggccggg ccgcccagag ccgccagcgg atctccgcgt tcctcggcag
     6061 cacccaactc ggcgagcagt tctcccgcgc cggccagccg atcgcggtgc tggtccggct
     6121 cgaccccgcg cgcaccgcct ccggctacgc ctggtccacc tccgacgggc cgccgttcgc
     6181 cctcgactcg atgacccagg ccaccgccgc cgtccacctg gccgcgcagc acccgatcga
     6241 ttggctgctc tcgtgaccgc ccccgacacc tccgccgcgc ccccgcagca cctcccgccc
     6301 gccggccgcg gccggcaccg gcccgagggc gccccgcccg cccgccgccg cagaccaccg
     6361 gccgccgccc ccaaggcgaa gaaggccgtg cgcaccccca cggtgctcca gatggaagcg
     6421 gtggagtgcg gcgccgcctc cctggcgatg gtcctggccc actacggacg gcacatcccg
     6481 ctggaggaac tgcggatcgc ctgtggcgtc tcgcgcgacg gctcccgtgc cagcaacctc
     6541 ctcaaggccg cccgcagtta cggcctgtcg gccaagggca tgcagatgga accagccgcc
     6601 ctcgccgacg tcgcggcacc ggccatcctc ttctgggagt tcaaccacta cgtcgtctac
     6661 gacggcccgg gccgccgcct cggccgccgc ggcgtccaca tcaacgaccc cgacaagggc
     6721 cgccgcttcg tgccggtgga ggacttcgac accagcttca ccggcgtggt gctggtcttc
     6781 gaacccaccg aggcgttccg cacgggcggt cgtcggccca gcgtgctcag cgccctgccg
     6841 gcccggctgc gcggcaccac cgggaccctg ctggcggcgc tgctcgccag cctgctgctg
     6901 gtcggcgtcg gcgccgcgct gccctcgctc agccgcacgt acatcgacat gttcctgatc
     6961 ggcaaccaga cctcactgct ggggccgctg ttcgccgcga tggccgcgat ggtggtgctc
     7021 accgccgtcc tgaccggcct ccaacaggcc aacctgctcc gcggccggat catctcctcc
     7081 accctcagca gcgcccgctt cctgcgccac ctgctgcgcc tgccggtcac cttcttcgcc
     7141 cagcgctcgc ccgccgacct cgtccagcgc ctgcagtcca acgacgcggt cgccgagacc
     7201 ctcgcccgcg acctggccgc ggcggccgtc gacggcgtgg tcgtcgtcct ctacgccgtg
     7261 ctgctgtgga cctacgaccc ccaactcacc gtcatcggcg tgctcatcgc gctgctcaac
     7321 gtcgtcgcca tgcggatcgt gatccggctg cgcgccaccc acacccagaa gctgcgcgcc
     7381 gacaccgccc gcctgaccaa cacctcctac accggcctcc agctcatcga gacgatgaag
     7441 gccaccggcg gcgagaacgg ctacttccgc cgctgggccg ggcagcacgc cgccaccctg
     7501 gaggaacagc agcggctcgg cgtccccagc gcctggctgg cggtcgtcgc ccccaccctc
     7561 gccaccctca acagtgcgct gatcctgtgg atcggcggcc tgcgcgccgt cgaaggccac
     7621 atctcgatcg gcctgttggt cgccttccag gccctggtga cccgcttcac cgccccgatc
     7681 acccggctca acggcgtcgc cggccggatc caggacttcg ccgcagacgt cgcccgcctc
     7741 aaggacgtcg agagcttccc cgccgacgcc cactactccc gcccggacgc ggacgccggc
     7801 acccgccgcc tcaaggggca cgtcaccctg gaggacgtca ccttcggcta cagcccgctc
     7861 gacgccccgc tgctgaccgg cttctcgctg gccgtcggcc ccggccagca ggtcgccctg
     7921 gtcggcggct cgggcagcgg caagtccacc gtctcccgac tgatctccgg cctctacgcc
     7981 ccttgggaag gcaccatccg gatcgacgga cagcgcctcg ccgacatccc gcgcagcgcg
     8041 ctggccgcct cggtctcctt cgtcgaccag gacgtcttcc tcttcgaagg caccgtccgg
     8101 gacaacgtcg cgctgtggga cccctccatc ccggacgacg cggtgatcgc cgccctccag
     8161 gacgccgccc tctacgacga cgtcatagcc cgccgccccg acggcatcca cagccgcgtc
     8221 gagcaggacg gccgcaactt ctccggcggt cagcggcagc gcctggagat cgcccgggcg
     8281 ctggtccggc gccccagcat cctcgtcctc gacgaggtca ccagcgccct ggacgccggc
     8341 accgaggcgg tcatcatgga caacctgcgg cgccggggct gtgcctgcgt catcatcgcc
     8401 caccggctga gcaccgtccg cgacagtgac gagatcgtgg tcctcgacca cggcgcggtc
     8461 gtcgaacgcg gccggcacgc ggaactggtc gccgccgggg gcgcctacgc cgagctggtc
     8521 aaggagcact gacgtggcat ccgtaccccc gtcgccggac gcggcacccg ccacggcacc
     8581 ggccaccgac gcggtcaccc acgccctggg cggcctcggc acccccgtgg accacaccgg
     8641 tctgcgcaac gtccccctcg aaggcccgca cgtcctgtgg ctggtcaccg gcggcaccct
     8701 cgacctgttc gcggtcgacg ccacccacga gggcccctgg cacttcctcg gccgcctcga
     8761 aaccggcacc ctgctgctcg gccccgtcga gggcgcccag cacactctcg tcggccgccc
     8821 gtcccaacac tgcggactgc gccggatcgc gctgcgggaa ctgccccgcc cggagcacgg
     8881 cggccacgcc gaccccggcg cgcatcccgg ggcgtacggc tacgccgacg cccacggggc
     8941 gccggccgcc agccccctgg agcacgcctt cgcgctcggt accgcccgcg gactgggcgt
     9001 gctgttcgag gcgccgctcg cgggccggcc caccgacgag gcggtcgccg acgacgacgt
     9061 gctgtggatg ccggtcccgc ccggcagcgt ccagtacggc gcctcctaca gcgccgaggc
     9121 cgccgccgac ctgctcgtcg acggtgccct gtggcagggg atggtcaacc agcagtaccg
     9181 gctgctgtcc gccgtcgacc gctggatcga agagctcgaa cgcgcccacg aggaccgggc
     9241 ggcggccggc atcaaggccg gcgaggccgt ccgggaacga gccgaccggg cgctgctggc
     9301 ctcgatcggc aaggaccgct ccggacgtgc cggcgcgggc cgcgaccggg ccggcgacga
     9361 cctgaccttc gcggtctgcc ggcaggtcgc ccaggccgcc ggcatcccgc tcaccgagcc
     9421 gcccaagggc gccgcggccg gcgaccggat cagcccggtc gaacgcatcg ccctggcctc
     9481 ccggatccgc acccgcgcgg ttcgcctcga cggcccctgg tggcgcaccg acgtcgggcc
     9541 cctggtgggc caccgcgcca agtccggcgc gccggtggcc ctgctgtggc ggcgcggccg
     9601 gtacgaagcg gtcaacccga cgtccgggct gcggatccgc atcggcaagg acaacgccga
     9661 ggagttcgaa ccgcgcgcgg tgatgttcta ccggccgctg ccggaccgcc ggctgacgct
     9721 gtggcggctg atgcgcttca gcctccgcgg gaaccgaacg gacctgcgca acctcgccct
     9781 cagcgggctg gtgacggtcg ccctcggcgc cctcgttccg atcgccaccg gcaaggtgct
     9841 cggcgtcttc gtaccggccg ccgcccgcgg cccgatcgtc caggtcgcgc tcgccgtgat
     9901 gatcgccggc atcgtcgcgg ccgccttcac cctgttgcag aacctcaccg tgctgcgcat
     9961 cgaaggccgc atcgagagcg tgctgcagcc cgccgtctgg gaccggctgc tgcgcctgcc
    10021 cacctcgttc ttcgccgccc gctccaccgg cgaactggcc agcgccgcga tgggcatcag
    10081 cgccatccgc caggtgctgg ccggggtcgg gccggtcgcc gtgcaggccg gcacggtcgg
    10141 cgcggtcaac ctcgcgctgc tgctcgtcta cagcgtcccg ctggcgctgg ccgcgttcgc
    10201 cgtgctgctc gtcatc
//
