LOCUS       NZ_CP119144            11321 bp    DNA     linear   CON 22-NOV-2023
DEFINITION  Streptomyces noursei strain C27 chromosome, complete genome.
ACCESSION   NZ_CP119144
VERSION     NZ_CP119144.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces noursei
  ORGANISM  Streptomyces noursei
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9627936)
  AUTHORS   Go,B. and Lu,Y.
  TITLE     Control effect and mechanism of Streptomyces NK27 against gray mold
            of grape
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9627936)
  AUTHORS   Go,B. and Lu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-FEB-2023) State Key Laboratory for Biology of Plant
            Diseases and Insect Pests, Institute of Plant Protection, Chinese
            Academy of Agricultural Sciences, Yuanmingyuan, Beijing 100193,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-OCT-2021
            Assembly Method                   :: ABySS v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 98x
            Sequencing Technology             :: Complete Genomics
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 14:00:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,442
            CDSs (total)                      :: 8,348
            Genes (coding)                    :: 8,203
            CDSs (with protein)               :: 8,203
            Genes (RNA)                       :: 94
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 46 of 145
            Pseudo Genes (incomplete)         :: 121 of 145
            Pseudo Genes (internal stop)      :: 12 of 145
            Pseudo Genes (multiple problems)  :: 30 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:17:19
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2398253
            Orig. end                         :: 2409574
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP119144.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11321
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="18"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11321
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11321
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6321](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6321
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            182..1267
                     /gene="zapE"
                     /locus_tag="P0D74_RS10670"
     CDS             182..1267
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_process="GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /gene="zapE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602439.1"
                     /locus_tag="P0D74_RS10670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cell division protein ZapE"
                     /protein_id="WP_275467543.1"
                     /transl_table=11
                     /translation="MVASPASGDGTTGPTALASRAPHVPAERLVAEMVPPPRFQGARFD
                     TYLPDLGQPSQAAAVEVLRAFAAGTGGADGDAGGRRRWFSRAPKKPAAPKGPRGVYLDG
                     GYGVGKTHLLASLWHATPAAPELKAFGTFVELTNLVGALGFQQTVRTLSEHRLLCIDEF
                     ELDDPGDTVLVSSLLGKLVEAGVALAATSNTLPGKLGEGRFAAADFLREIQGLSAHFRA
                     LRIDGEDYRHRGLPEAPAPFDDETVRRAADRTPGASLDDFPTLLDHLSKVHPSRYGAMC
                     DGITAVCLTDVQAVPDQSTALRLVVLADRLYDREVAVLASGKPFDALFSDEMLRGGYRK
                     KYFRAISRLTALARDAKTLVA"
     gene            complement(1282..1488)
                     /locus_tag="P0D74_RS10675"
     CDS             complement(1282..1488)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675663.1"
                     /locus_tag="P0D74_RS10675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_275462492.1"
                     /transl_table=11
                     /translation="MPHPEPHPAPEPSEPSDQRPAAADPAAGPPPADCVECGKPTAYPA
                     TTPGIVRCPVCEWQEAQRTACSG"
     gene            complement(1542..2222)
                     /locus_tag="P0D74_RS10680"
     CDS             complement(1542..2222)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576543.1"
                     /locus_tag="P0D74_RS10680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter"
                     /protein_id="WP_275462493.1"
                     /transl_table=11
                     /translation="MTAPLTALVRYQAALLAGSYRWLPPLLVHAAFLAVGIQTGGPILD
                     AYGYAAGGLLPVTVWLTRICVTNEPSAARSCVAAATGPGRAHLAALLVAAGAALLLALV
                     GTAFVALLSDPHSTGHRVAVPVLPATVAGLCTALVCVVLGTAIGALCSRPVLLRPGWGV
                     PTGLSAALLVLVLGASPANAALTGMITGSERGTVTVPWLPLAAALLLASGAAAAACALA
                     GRRG"
     gene            complement(2219..3130)
                     /locus_tag="P0D74_RS10685"
     CDS             complement(2219..3130)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536943.1"
                     /locus_tag="P0D74_RS10685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_275462494.1"
                     /transl_table=11
                     /translation="MPWQTGQRMRLDGVGRRYGLRGPWVLREVRLDVPAGALLRVEGAN
                     GSGKSTLLRLLAGIDRPTVGRITGRPRTAYVPERFPAALPFTAAGYLTHLGRVHGLRTA
                     EATGRAAEWLARFGAAGHARTPMAELSKGTSQKVAVAQALLADPELLVLDEAWTGLDPA
                     ARDVLDRAVAERVAAGGTVVFVDHDPRRLAGAADGRFRVADGRLRSEAPAVAADAPREA
                     GPRTVIEAEGPPGAAPPTDLPGAPDLAPGPGGALRLTVPAAHSDALLRTLLTARPPWHI
                     RAVTAAQEPGAHAAVPGGNPRP"
     gene            3379..4041
                     /locus_tag="P0D74_RS10690"
     CDS             3379..4041
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536944.1"
                     /locus_tag="P0D74_RS10690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_275467544.1"
                     /transl_table=11
                     /translation="MLPLVVRLERDAPPGRTDALETAARAVLVLLSDERALGTGEWAQV
                     VHDWQDSRIRKVVRRARGAEWRRAAALPGITVSGREAEVRVFPPVPLDGWPKDLARLQV
                     SGTELADPRAPGEPAAGAPVLWLNPAVQMSAGKAMAQAGHGAQLAWWALSAADREAWRA
                     AGFPLAVRTAAPAAWTGLTGSGLPVVRDAGFTEIAPGSCTVVADHPALRGTALPAHH"
     gene            4109..4783
                     /locus_tag="P0D74_RS10695"
     CDS             4109..4783
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016578488.1"
                     /locus_tag="P0D74_RS10695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysE family translocator"
                     /protein_id="WP_275462495.1"
                     /transl_table=11
                     /translation="MLTSLIGFTVVAALLTVSPGPDFAVVLRTALGSGRRAALCAALGI
                     AAGCFVWGLAGAVGLTAMLAASKAAYDAVRIAGALYLVWLGVQALRSARARRGTDGSAP
                     APDAAAANAPGDGGAPAVPGGPWRAFRGGLLTNVLNPKVGVVYLSLLPQFIPHGAPVVA
                     TTMLLVTVHAVLGVLWLGGVALAVHRARAVLQRPRVRRRLDQATGGVLVALGAAVGFEV
                     AS"
     misc_feature    4883..4897
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 26.07"
                     /tool="antismash"
     gene            5001..6321
                     /locus_tag="P0D74_RS10700"
                     /pseudo=""
     CDS             5001..6321
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643869.1"
                     /locus_tag="P0D74_RS10700"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 5.2e-96, bitscore: 319.9,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLGTGIGWRPEIAAEIAALPGVDWVEVVAENVCPGHLPDALGEL
                     RERGTTVVPHGVSLGLGGADRPDADRLAALAERATALDAPLVTEHLAFVRAGGPLTASP
                     VLEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLALENIAALFAWPGEELTEGRFLAE
                     LVERTGVRLLIDVANLHTNHVNRGEDPAAALDELPVEAIAYVHVAGGVERDGVWHDSHA
                     HPVTQPVLDVLAELCARIDPPGVLLERDDDFPEGGALAAELDAIRAVVEGARRVPAA"
     gene            6331..7332
                     /locus_tag="P0D74_RS10705"
     CDS             6331..7332
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="P0D74_RS10705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_275462496.1"
                     /transl_table=11
                     /translation="MSFTQGVYLGLGLSSAALVFGALLVRLRTPKARLVDSPTNDVREL
                     AFLAGGPARVAETVLAVMHEDGRLTVGGPGLVTVRRAAAHDPVEAAVLDAVAATPGGAL
                     TTVRERVMRSPAVQDVGDRLAARGLLRAPRATRGWRRWAGLQVKACGALFCVAMLLSFL
                     GVRDGGGPWAPPVVLRIVPAVAVGLVVGLVCRRSFGRRITRAGRSALAAARPRARTPSG
                     FRDAEAAWQVAGLAVALGGAAVLTDEVLRDHFVQAKAALVGSSGGASSAAGDSGGSGGA
                     PAWCGGSSDGGGGSGCGASSCGGGSSCGGSSGCGGGSGCGGGGCGGGGCGGG"
     gene            7460..8302
                     /locus_tag="P0D74_RS10710"
     CDS             7460..8302
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016577176.1"
                     /locus_tag="P0D74_RS10710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_275462497.1"
                     /transl_table=11
                     /translation="MWLLFLLLAWVVAIVSCVRVCRTALAAGLALHRGSDATADADARA
                     DDTGADAGLTLYETAYLAGGPHRLADVVLVLMAQQRRLHLAHTGWATVVDPVGQDPVER
                     AALRAIGPEGQRRIPAVRRALTSAEAVRGLADRLVHAGLAAPAAVRTGIGTAVRHVQFA
                     ALLVLLAAGATYWAAPSGEVDRLVLAWFAFPLVLTLGTWAVARAEAHPYTAWATEAGSA
                     LLRHEHPGGPSSRPGPPPAEPTGLTGPTGPTGLTGLALFGPTALADPGLRAALHSSGA"
     gene            8447..10156
                     /locus_tag="P0D74_RS10715"
     CDS             8447..10156
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (91-295). E-value: 1e-17.
                     Score: 56.5. Matches aSDomain:
                     nrpspksdomains_P0D74_RS10715_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016570335.1"
                     /locus_tag="P0D74_RS10715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_275462498.1"
                     /sec_met_domain="PF00561 (E-value: 4.8e-24, bitscore: 84.3,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRALALYGALGSLVLTGLASPPAGGAVRDPAAPPAPVHPLAFGRC
                     AAVEHLPPTVECGTLTVPLDYARPNGKKIKLTVSRARATEPGARQGALVFNPGGPGASS
                     MQFPLYGALPHWQRTARAYDFVGYAPRGVGRSAPLSCQNPQEFAKAPTDSARYPSDAFK
                     RQKVAEARAYARGCAQRAGADLPFYNSINNARDLDMLRAALGEKKLTFMGASYGTYFGA
                     VYATLFPGHVRRMIFDSVVNPEPRQIWYRNNLDQSLAFERRWHDWLRWVAKHHDTYHLG
                     THQEAVQHSYDTALARLRRTPLNGKIGPAQLQGAFLKTGYNDAFWSMRADALSKYLHGD
                     AKPLTAQAMPSGDGKDEENANAVYTAVECNDAAWPRDWRTWDRDNTSLARTAPFETWDN
                     VWMNLPCAFWPDRRESAADGAEEALRTATDRAAKGGANGGELADAYRNAAATELDDVLR
                     GQHGPLNIRTEPGALPPVLLLAAERDAATPYPGALELQRRLPGSSLVTERGAGTHGVAL
                     GDNACVNAHVEAYLLRGKVPGHRVYCAPRAEPVPGQPLRRARPSLIPTVKGR"
     misc_feature    complement(8474..8493)
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 23.45"
                     /tool="antismash"
     aSDomain        8720..9331
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_P0D74_RS10715_Abhydrolase_1.1"
                     /evalue="1.00E-17"
                     /label="P0D74_RS10715_Abhydrolase_1.1"
                     /locus_tag="P0D74_RS10715"
                     /protein_end="295"
                     /protein_start="91"
                     /score="56.5"
                     /tool="antismash"
                     /translation="ALVFNPGGPGASSMQFPLYGALPHWQRTARAYDFVGYAPRGVGRS
                     APLSCQNPQEFAKAPTDSARYPSDAFKRQKVAEARAYARGCAQRAGADLPFYNSINNAR
                     DLDMLRAALGEKKLTFMGASYGTYFGAVYATLFPGHVRRMIFDSVVNPEPRQIWYRNNL
                     DQSLAFERRWHDWLRWVAKHHDTYHLGTHQEAVQHSYDTAL"
     gene            complement(10246..10983)
                     /gene="hemQ"
                     /locus_tag="P0D74_RS10720"
     CDS             complement(10246..10983)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536947.1"
                     /locus_tag="P0D74_RS10720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_275462499.1"
                     /transl_table=11
                     /translation="MDDMTDASTPDSAKTPNAGKKAKDLNEVIRYTLWSVFKLRDVLPE
                     DRSGYADEVEELFAQLAAKDVVVRGTYELSGLRADADVMIWWHSENSDDLQEAYNRFRR
                     TRLGRALEPVWSNMALHRPAEFNKSHIPAFLADEHARDYVSVYPFVRSYDWYLLPDEDR
                     RRMLADHGKMARGFPDVRANTVASFSLGDYEWILAFEADELHRIVDLMRHLRASEARRH
                     VREEIPFYTGRRKPVAELVAGLA"
ORIGIN
        1 gtcggcgccc tccgggacgt ccacgagggc gtccggggcc ggtgcgggcg gggcgggaag
       61 ggggaacagg cgtcgcatgg gcgcagtgtg acatgcctcg ctcagcgggt cgggtggtgc
      121 gtcacagcga ctaggctgga cggctgtgtc accttcccag tcctcctccg ctccctcccc
      181 gatagtcgcg tccccggcct cgggggacgg caccacgggc cccaccgcgc tcgcctcccg
      241 cgcaccgcac gtcccggccg agcgcctggt cgccgagatg gtgccgccgc cgcgcttcca
      301 gggcgcccgc ttcgacacct acctcccgga cctgggacag cccagccagg ccgccgccgt
      361 ggaggtcctg cgggccttcg ccgcgggcac cggcggggcg gacggggacg ccggcgggag
      421 gcggcgctgg ttctcccgcg caccgaagaa gcccgccgcc cccaagggcc cgcgcggggt
      481 ctacctggac ggcggctacg gcgtaggcaa gacccacctg ctcgcctccc tgtggcacgc
      541 gacccccgcg gcccccgagc tcaaggcgtt cggcaccttc gtcgagctga ccaacctggt
      601 cggcgcgctc ggcttccagc agaccgtgcg cacgctcagc gaacaccgcc tgctgtgcat
      661 cgacgagttc gagctggacg accccggcga cacggtgctg gtctccagcc tgctcggcaa
      721 gctggtggag gccggcgtcg cgctggccgc cacctccaac acgctgcccg gcaagctcgg
      781 cgagggccgg ttcgcggccg ccgacttcct gcgcgagatc cagggcctga gcgcccactt
      841 ccgcgccctg cgcatcgacg gcgaggacta ccgccaccgc ggtctgcccg aggcgcccgc
      901 cccgttcgac gacgagacgg tccgccgcgc ggcggaccgc acccccggcg cctcgctcga
      961 cgacttcccg accctgctgg accacctgtc gaaggtgcac cccagccggt acggcgcgat
     1021 gtgcgacggc atcacggcgg tctgcctcac cgacgtccag gcggtgccgg accagtcgac
     1081 cgcgctgcgc ctggtggtgc tcgccgaccg gctctacgac cgcgaggtgg cggtgctggc
     1141 ctccggcaag cccttcgacg cgctgttcag cgacgagatg ctgcgcggcg gctaccgcaa
     1201 gaagtacttc cgcgccatct cccgtctgac cgcgctggcc cgggacgcca agacgctggt
     1261 cgcgtaggcg ccgccgggcg gtcagcccga gcaggccgtg cgctgggcct cctgccactc
     1321 gcagaccggg cagcggacga tgccgggagt ggttgccggg taggcggtcg gcttgccgca
     1381 ctcgacgcag tcggcgggcg gcggccccgc ggcggggtcg gccgccgccg gacgctggtc
     1441 ggacggctcg gacggctcgg gcgcggggtg cggctcgggg tgcggcatgc gcccgaccct
     1501 acgccgcggc accgagcccc ggaccggccg gagcggaccg ctcagccgcg gcgtccggcc
     1561 agggcgcagg ccgcggccgc ggcacccgag gcgagcagca gcgcggcggc gagcggcagc
     1621 cacgggacgg tgacggtgcc gcgctcggag ccggtgatca tgccggtcag tgcggcgttg
     1681 gcgggcgacg cgcccagaac gagcacgagc agcgccgcgc tcaacccggt cgggacgccc
     1741 caaccggggc gcagcagcac gggccgggaa cacagcgcac cgatcgcggt gcccagcacc
     1801 acgcagacca gcgccgtgca caacccggcg accgtcgcgg gcagcaccgg gacggcgacc
     1861 cggtgaccgg tggagtgcgg atcggagagc agcgccacga aggcggtgcc cacgagcgcg
     1921 aggagcagtg cggcgccggc cgcgaccagc agggcggcca ggtgcgcccg gccggggccg
     1981 gtggcggccg cgacgcaact gcgggcggcg gacggctcgt tggtgacgca gatccgggtc
     2041 agccagacgg tgacgggcag cagtccgccg gcggcgtagc cgtaggcgtc caggatcggg
     2101 ccgccggtct ggataccgac ggccaggaac gccgcgtgga ccaggagcgg cggcaaccag
     2161 cggtaggacc cggcgagcag cgccgcctgg taccggacca gggcggtcaa cggggcggtc
     2221 atgggcgggg gttccctccg gggacggcgg cgtgggcgcc gggctcctgg gcggcggtga
     2281 ccgcgcggat gtgccagggc gggcgcgcgg tcagcagggt gcgcagcagg gcgtcggagt
     2341 gggcggccgg gacggtcagc cggagggcgc cgcccgggcc gggcgccagg tccggggcgc
     2401 cggggaggtc ggtcggcggc gcggcgcccg gcgggccctc cgcctcgatg acggtccgcg
     2461 gccccgcctc ccggggcgcg tcggcggcca cagcgggggc ctcggaccgc agccgcccgt
     2521 ccgcgacgcg gaagcggccg tccgcggccc cggccagtcg gcgcggatcg tggtcgacga
     2581 agacgacggt gccgccggcg gcgacccgtt cggcgaccgc gcggtccagg acgtcccggg
     2641 ccgccgggtc caggcccgtc cacgcctcgt ccaggacgag gagctcgggg tcggcgagca
     2701 gggcctgggc tacggcgacc ttctggctcg tccccttgga cagttcggcc atcggggtgc
     2761 gggcgtgccc ggcggcgccg aaacgggcca gccactcggc ggcccggccg gtcgcctccg
     2821 cggtgcgcag cccgtggacc cggccgaggt gggtgaggta gccggcggcg gtgaacggca
     2881 gggccgccgg gaaccgctcg gggacgtagg cggtgcgcgg gcgcccggtg atccggccga
     2941 ccgtgggccg gtcgatgccc gcgagcagcc gcaacagggt ggacttcccg ctgccgttgg
     3001 caccctcgac gcgtagcagc gcgcccgccg gcacgtcgag ccgcacctcg cgcagcaccc
     3061 agggcccgcg cagcccgtaa cgtcgcccca ccccgtccag ccgcatccgc tgccccgtct
     3121 gccacggcat cgaccgcacc tcccccgcgc ccccgccggt accccggccc tcgccgacgg
     3181 tagccgggga cggggcgcgc cggggtgccg ggagcggatt ccgcgaccgc gggcggtcgc
     3241 cggggactgt cagtggcggc tggcagactg aagcacgtga ccacgaccga gaccgccggc
     3301 caggccggcg cgcaccacgc accccaccag accgacgccc cgcggccgga ccgccccgag
     3361 gagagcgcac cggagtacgt gctgccgctc gtggtgcgcc tggagcggga cgccccgccc
     3421 gggcggacgg acgcgctgga gaccgccgcg cgggcggtgc tggtgctgct ctcggacgag
     3481 cgggccctcg ggacgggcga gtgggcccag gtggtgcacg actggcagga ctcccggata
     3541 cgcaaggtgg tgcggcgggc gcgcggtgcg gagtggcggc gggccgcggc cctgcccggc
     3601 atcacggtca gcggccggga ggccgaggtg cgggtcttcc cgccggtgcc gctggacggc
     3661 tggccgaagg acctggcgcg gctccaggtc tccgggaccg agctggccga tccgcgggcc
     3721 cccggggagc cggcggccgg cgccccggtg ctgtggctca atccggcggt tcagatgtcg
     3781 gcgggcaagg cgatggcgca ggccgggcac ggtgcgcaac tggcgtggtg ggcgctgtcc
     3841 gcggcggacc gggaggcgtg gcgcgcggcg gggttcccgc tggccgtgcg gaccgcggca
     3901 cccgccgcgt ggacggggct caccggcagc gggctgcccg tggtgcggga cgccggcttc
     3961 acggagatcg cgccgggctc gtgcacggtg gtcgccgacc atccggcgct gcgcggtacg
     4021 gcgctgccgg cgcaccactg agcggggcgg ggcggggtgc cgctcccccg ccggcggtcg
     4081 ggtgcaggtc gggacatacc gtgtgcccat gctgacctcg ctcatcggct tcaccgtggt
     4141 cgccgcgctg ctgaccgtct cccccggccc cgacttcgcg gtggtcctgc ggaccgcgct
     4201 cggctcgggt cgccgggccg cgctgtgcgc cgcgctcggc atcgccgccg gctgcttcgt
     4261 gtgggggttg gccggggcgg tggggctgac cgcgatgctc gccgcctcga aggcggcgta
     4321 cgacgcggtg cggatcgccg gtgcgctgta tctggtgtgg ctcggggtgc aggcgttgcg
     4381 cagtgcccgg gcccggcgcg gcacggacgg gtccgcgccg gcaccggacg cggcagcggc
     4441 gaacgcgccg ggggacggcg gggcgcccgc ggtcccgggc ggcccgtggc gggcgttccg
     4501 cggcggcctg ctgacgaacg tcctcaatcc caaggtgggc gtggtctacc tgtccctgct
     4561 gccacagttc atcccgcacg gcgcgccggt ggtcgccacc acgatgctgc tggtgaccgt
     4621 gcacgcggtg ctgggcgtgc tgtggctggg cggggtggcc ctggcggtgc accgggcgcg
     4681 ggcggtgctg cagcgcccgc gggtgcgacg gcggctggac caggccaccg gcggggtgtt
     4741 ggtggcgctc ggcgctgcgg tcgggttcga ggtcgccagc tgagcgacgc ccgcccggga
     4801 ccggcttgct ggcttgctgg cttgctggct tgctggcttg ctggcttgct ggcggacgaa
     4861 cggaacagga gggacgggac gggggccgtc cggcccgtac tgggccaagc tcaaatcaac
     4921 ctctgaaccg ctcggccgtg cggttagccg gcccgcgcgt ggtgaggagt accccgacca
     4981 ggggacgaga agggggagcc gtgcgcttgg ggacgggaat cggctggcgg ccggagatcg
     5041 ccgcggagat cgctgcgctg cccggggtgg actgggtgga ggtggttgcc gagaacgtct
     5101 gtcccggcca tctcccggac gcccttgggg agttgcgcga gcgcggcacc acggtggtcc
     5161 cgcacggggt gtcgctcggg ctcggcggcg cggaccggcc ggacgccgat cggttggccg
     5221 ccctcgccga gcgggccacc gcgctggacg ccccgttggt caccgagcac ctcgcgttcg
     5281 tccgggccgg cggcccgctg accgcgtcgc cggtgctgga ggcggggcac ctgctgcccg
     5341 tgccgcgcac ccgggacgcc ctcgacgtgc tgtgcgagaa cgtccggatc gcccaggacg
     5401 cgctcccggt gccgctggcc ctggagaaca tcgccgcact gttcgcctgg cccggcgagg
     5461 agctgaccga gggccggttc ctggcggagc tggtcgagcg caccggagtg cgcctgctca
     5521 tcgacgtcgc caacctccac accaaccacg tcaaccgcgg cgaggacccg gccgcggccc
     5581 tcgacgaact gccggtcgag gcgatcgcct acgtccacgt cgcgggcggc gtcgagcgcg
     5641 acggggtgtg gcacgacagc cacgcccacc cggtgaccca gccggtcctg gacgtcctcg
     5701 cggagctgtg cgcgcgcatc gatccgcccg gcgtgctgct ggagcgggac gacgactttc
     5761 ccgagggcgg ggcgctggcc gccgaactcg acgcgatccg cgccgtggtg gagggggccc
     5821 gtcgtgtccc ggcagcctga ggagccgacg gggacggggg ccgggcgtcc gccgggggac
     5881 accgctgcgg cccgcgagcg gctggccctg gcgcaggccg cgctgctgtc ggcactggtg
     5941 gccgggacgc cggtgccgga ggggttcgac cgggcgcggc tgcgggtgca gggccgggcg
     6001 ctgaccgcca agcgggcgtc ggtggtggcc aaggtcgcgc ccgagctgcc ggagttcctc
     6061 ggcgacggct accgggcggc cttcctcggc tacgcccagg accgcccgat gcacggcggc
     6121 taccgccagg acgccctgga cttcgccgcg ttcctgctcg cccagggcgc gcccgaggac
     6181 ccggtggcgc ggcagcggct gaccgactgg tggcgcgacc gggccggccc gcgaccgccg
     6241 tccgggcacc ccgtgccccg gctcgcgcgg gccgccgccc gccgggtccg gactctgcgg
     6301 cgcgccccgc ggcgcgggtg aggcggcgtc gtgtccttca cgcaaggggt gtatctgggg
     6361 ctcggcctgt cgtcggcggc actggtcttc ggcgcgctgc tggtccggtt gcggacgccg
     6421 aaggcccggc tggtcgactc gccgaccaat gacgtccgcg agctcgcgtt cctggccggt
     6481 gggccggcac gggtggccga gaccgtgctg gcggtgatgc acgaggacgg gcgactgacg
     6541 gtcggcggcc cgggcctggt gacggtgcgg cgggccgccg cgcacgaccc ggtcgaggcg
     6601 gcggtgctgg acgcggtcgc cgccaccccg ggcggggcgc tgaccaccgt ccgggagcgg
     6661 gtgatgcgca gcccggccgt gcaggacgtc ggcgaccggc tggcggcccg gggcttgctg
     6721 cgggccccgc gcgccacccg gggttggcgg cggtgggcgg gcctccaggt gaaggcgtgc
     6781 ggcgcgctgt tctgcgtcgc gatgctgctg tccttcctcg gcgtccgcga cggcgggggt
     6841 ccgtgggcac cgccggtggt gctccggatc gttccggcgg tggccgtcgg gttggtggtc
     6901 ggcctggtgt gccggcggtc gttcggccgg cggatcaccc gggcgggcag gtccgcgctc
     6961 gccgcggccc gcccccgggc caggacgccc tccgggttcc gggacgccga ggcggcctgg
     7021 caggtcgcgg ggttggcggt ggcgctgggc ggtgccgcgg tgctcaccga cgaggtgctg
     7081 cgggaccact tcgtgcaggc gaaggcggcg ctggtggggt cgtcgggcgg cgcgtcgtcc
     7141 gcggccggcg actccggcgg ttccggcggg gccccggcgt ggtgcggtgg gtcctcggac
     7201 ggcggcgggg ggtccggctg cggtgcgtcc tcgtgcggcg gcgggtcgtc gtgcggtggg
     7261 agttcgggtt gcgggggcgg ctccggatgc ggcggcggag ggtgcggcgg gggcggctgc
     7321 ggcggcgggt gacgcggcgc cggcccgaag tgccgggccg gtgcgggcgg ttcggcctgc
     7381 accgacgcat ctcggccatc cgtccgcgct gcgagaaact ccttggcggc aggggccgcc
     7441 gtcccgtaga acctccgcca tgtggctcct cttcctcctg ctcgcctggg tcgtcgcgat
     7501 cgtctcctgc gtccgggtct gccgcacggc gctggccgcc gggctggccc tgcaccgcgg
     7561 ctcggacgcg actgcggacg ccgacgcgcg ggccgatgac accggtgccg acgccggtct
     7621 gaccctgtac gagacggcct acctcgccgg cggcccgcac cgactcgccg acgtggtact
     7681 ggtcctgatg gcccagcagc gacggctgca cctcgcccac accggctggg ccaccgtcgt
     7741 ggacccggtc ggtcaggacc ccgtcgagcg ggccgcgctg cgcgcgatag gccccgaggg
     7801 acagcgccgc ataccggccg tgcggcgcgc gctgacgagc gccgaggcgg tccgcgggct
     7861 ggccgaccgg ctggtccatg ccgggctggc ggcccccgcc gcggtccgca ccgggatcgg
     7921 caccgcggtc cgccacgtgc agttcgccgc cctcctggtg ctgctggccg ccggcgccac
     7981 ctattgggcg gcgccgtccg gcgaggtcga ccggctggtg ctggcctggt tcgccttccc
     8041 gctggtgctg accctcggca cctgggccgt cgcccgcgcc gaggcccacc cgtacaccgc
     8101 gtgggccacc gaggccggtt cggcgctgct gcggcacgag caccccggcg gaccgtcgag
     8161 ccgtccgggc ccgccgccgg cagagccgac cggactgacc gggccgaccg ggccgaccgg
     8221 gctgaccggg ttggcgttgt tcgggcccac cgcgctcgcc gatcccggtt tgcgcgcggc
     8281 gctccactct tccggggcat agcgtccgac accgccgtag ggtgctttac ttcgccacca
     8341 attggcccaa ctatcccttt tgtatgccca ccgtgcggcc ccgtgcgggc gagccccact
     8401 ccggagcccc gcaccgcgca ccgcgcacca tacgaaggga cggctcgtga gagcacttgc
     8461 gttgtacggc gccctcgggt cgctggtcct gaccggcctc gcctccccac cggccggcgg
     8521 cgccgtccgg gacccggccg cgccgcccgc ccccgtccac cccctcgcct tcggccggtg
     8581 cgcggccgtg gagcacctgc cgcccaccgt cgaatgcggc accctcaccg tcccgctcga
     8641 ctacgcgcgc cccaacggca agaagatcaa gctgacggtc agccgggccc gggccaccga
     8701 gccgggcgcg cgccagggcg ccctggtctt caaccccggc ggacccggcg cgtccagcat
     8761 gcagttcccg ctctacggtg cgctgccgca ctggcagcgc accgcccgcg cctacgactt
     8821 cgtcggctac gcgccgcgcg gggtggggcg cagcgccccg ctgtcgtgcc agaacccgca
     8881 ggagttcgcc aaggccccca cggacagtgc gcggtacccc agcgacgcct tcaagcggca
     8941 gaaggtggcg gaggccaggg cgtacgcccg cggctgcgcc caacgggccg gcgccgacct
     9001 gccgttctac aactcgatca acaacgcccg ggacctggac atgctgcggg cggcgctcgg
     9061 cgagaagaag ctgacgttca tgggcgcgtc ctacgggacg tacttcgggg cggtctacgc
     9121 gacgctgttc cccggccacg tgcgtcggat gatcttcgac agcgtggtca acccggagcc
     9181 ccggcagatc tggtaccgca acaacctcga ccagagcctc gccttcgaac gccgctggca
     9241 cgactggctg cgctgggtcg ccaagcacca cgacacctac cacttgggca cccatcagga
     9301 ggccgtgcag cactcctacg acaccgccct ggcacggctg cggcgcaccc cgttgaacgg
     9361 gaagatcggg ccggcgcagt tgcagggggc gttcctcaag accggctaca acgacgcgtt
     9421 ctggtcgatg cgcgcggacg ccctgtcgaa gtacctgcac ggcgacgcca agccgttgac
     9481 cgcgcaggcg atgccctccg gcgacggcaa ggacgaggag aacgccaacg cggtctacac
     9541 cgccgtcgag tgcaacgacg cggcctggcc gcgcgactgg cgcacctggg accgggacaa
     9601 cacctccctc gcccgcaccg cgccgttcga gacctgggac aacgtctgga tgaacctgcc
     9661 gtgcgccttc tggccggacc gccgggagtc ggcggcggac ggcgccgagg aagcgctgcg
     9721 cacggccacc gaccgggcgg ccaagggcgg ggcgaacggc ggcgaactgg ccgacgccta
     9781 ccggaacgcc gccgccaccg aactggacga cgtcctgcgc ggccagcacg gcccgctgaa
     9841 catccgcacc gagcccggcg cgctgccgcc ggtgctgctg ctggccgccg aacgggacgc
     9901 ggccacgccg tacccgggcg cgctggagct tcagcgccga ctgcccggct cgtcgctggt
     9961 caccgagcgg ggcgcgggca cccacggcgt cgcgctcggc gacaacgcct gcgtgaacgc
    10021 ccacgtcgag gcgtacctgc tgcgtgggaa ggtccctggg caccgggtgt actgcgctcc
    10081 gcgggccgag ccggtgccgg ggcagccgct gcggcgggcc cggccgtcgc tgatcccgac
    10141 ggtgaagggg cggtagccgc cccgtccccg tccccggcgt ccacgaccga cgggccgtcc
    10201 ctgcacgggg cggcccgtcc gtcgtgtgcc cggggtgccg gccggtcagg ccaggccggc
    10261 gaccagctcg gcgaccggct tgcggcggcc ggtgtagaag gggatctcct cgcggacgtg
    10321 gcgccgcgcc tcggaggcgc gcaggtggcg catcaggtcc acgatgcggt gcagctcgtc
    10381 ggcctcgaag gcgaggatcc actcgtagtc gccgagcgag aacgacgcga cggtgttggc
    10441 gcggacgtcg gggaagccgc gggccatctt gccgtggtcg gcgagcatcc ggcggcggtc
    10501 ctcgtcgggg aggaggtacc agtcgtagga gcgcacgaac gggtagacgc tcacgtagtc
    10561 gcgggcgtgc tcgtcggcca ggaaggccgg gatgtgcgac ttgttgaact cggcggggcg
    10621 gtgcagcgcc atgttcgacc agacgggctc cagcgcgcgg cccaggcggg tgcggcggaa
    10681 gcggttgtac gcctcctgga ggtcgtcgga gttctccgag tgccaccaga tcatcacgtc
    10741 ggcgtcggcg cgcagaccgg agagttcgta ggtgccgcgg accaccacgt ccttggcggc
    10801 gagctgcgcg aacagctcct cgacctcgtc ggcgtagccg gaccggtcct cgggcaggac
    10861 atcgcgcagc ttgaagaccg accagagggt gtaacggatg acctcgttga ggtccttggc
    10921 cttcttgccg gcgttgggag tcttggcgga gtcgggggtg gaagcgtctg tcatgtcgtc
    10981 cattctcact cgccgccggc ggagcccgcc gccagggtcc cgaggatctc gtcggcggcc
    11041 cggccggcgg agccgatgca cgcggggatg ccgacgccgt cgtagagcgc gccgcagacc
    11101 cgcagcgggc ccggcaggcc ggcgaccgcg gcccggatgc gggccgtgcg gtccacgtgg
    11161 ccgacggcgt actgcggcag cccgccgtcc cagcgggtga ccgtggacgc caccggccgg
    11221 gccgccagcc cgaccgcctc accgaggtcc gccagggaga ggccgaccag ttcgtcgtcg
    11281 tcgcgtccca ggtcggcctc gtcgccgtgg cggccgaggg a
//
