LOCUS       NZ_CP119144            11194 bp    DNA     linear   CON 22-NOV-2023
DEFINITION  Streptomyces noursei strain C27 chromosome, complete genome.
ACCESSION   NZ_CP119144
VERSION     NZ_CP119144.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces noursei
  ORGANISM  Streptomyces noursei
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9627936)
  AUTHORS   Go,B. and Lu,Y.
  TITLE     Control effect and mechanism of Streptomyces NK27 against gray mold
            of grape
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9627936)
  AUTHORS   Go,B. and Lu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-FEB-2023) State Key Laboratory for Biology of Plant
            Diseases and Insect Pests, Institute of Plant Protection, Chinese
            Academy of Agricultural Sciences, Yuanmingyuan, Beijing 100193,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-OCT-2021
            Assembly Method                   :: ABySS v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 98x
            Sequencing Technology             :: Complete Genomics
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 14:00:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,442
            CDSs (total)                      :: 8,348
            Genes (coding)                    :: 8,203
            CDSs (with protein)               :: 8,203
            Genes (RNA)                       :: 94
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 46 of 145
            Pseudo Genes (incomplete)         :: 121 of 145
            Pseudo Genes (internal stop)      :: 12 of 145
            Pseudo Genes (multiple problems)  :: 30 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:17:19
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2069867
            Orig. end                         :: 2081061
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP119144.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11194
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="16"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11194
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11194
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6194](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6194
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(746..2167)
                     /locus_tag="P0D74_RS09100"
     CDS             complement(746..2167)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020548159.1"
                     /locus_tag="P0D74_RS09100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF6605 domain-containing protein"
                     /protein_id="WP_275462246.1"
                     /transl_table=11
                     /translation="MTAPSSAVAASAGAVVEGYPSDASVRAGDTLRLHVSTHAPWFRAD
                     FYRCGAQIRHMGSIRGPGRHAPPGRHDADWDWPHHDFPVPEDWPSGVYLAVLGPDAAPP
                     APVPSCAEALDARRSRLLFVVRAREPARTTGILYKVPLFTYHAYNRSGGGCLYGSPPVS
                     ASGKATGEFTVSLRRPGGGTGGPVKGHPDAHAPQTPRQTFAHWDAPFIAWLEAAGFTAE
                     YCTDLDLHQTPHLTDGYRLLISAGHDEYWSADTRRNVTAFRDRGGNIAFFGANTCWWRV
                     AVSDDGATARCAKYPPGAREALDVDGTPGAPDHWWESAPENALTGVSYRNGGGYWEGSR
                     PPLGFTVQCTEHPVFAGTGLSDGDILGAAHSLVGYECDGAAHVRDHRGRAVPTGTDGTP
                     TDFTILGVADLPLGPASGWQVPAREDPDPARAATFGLHTKGGTVFTAATTDWARLLSLD
                     PQVATLTHNVLTLLS"
     gene            complement(2164..3315)
                     /locus_tag="P0D74_RS09105"
     CDS             complement(2164..3315)
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012744.3"
                     /locus_tag="P0D74_RS09105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="WP_275462247.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 1.3e-15,
                     bitscore: 56.3, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRIAYLHGGSMPSTFANGVHVMRMCDAFSAAGHDVTLYSLPGPQL
                     AADPYAYYGVRHRFPIRLVSAPDYTPHGYWKRADRVRDLLIRDLPDLVYGREPYSLSRA
                     ADLAPFVYEVHQLRSDLAPPEMEHDLLAQPLLSRVVVITHALARDFQQAHAEAGTLPLL
                     VAPDGADPVEPVPTTAVPELPGRPNVPRIGYVGHLYEGRGIGLIVELAGRLPGFDFHLV
                     GGTTGDRDRWDTSCDLPNVHFHGHRPPAELPYYYPHFDVVLAPYQHKVYTWGKLSETGQ
                     WASPMKVFEYMAHGHPMIASDLPVLREVLQDRVNCLLRPPDDADAWADALSELVADRAL
                     RQALGDEARRQVLDRYTWRRRADVVLAGLPLHDRSDEQSVSPT"
     gene            4018..5001
                     /locus_tag="P0D74_RS09110"
     CDS             4018..5001
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04267.2"
                     /locus_tag="P0D74_RS09110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HEXXH motif-containing putative peptide
                     modification protein"
                     /protein_id="WP_275462248.1"
                     /transl_table=11
                     /translation="MRVEPDAQQSAEERTALARTTRSALARAELHVTPAEATHPAIIEI
                     AHLAQHVGRADSPDRERLDRLGRALDRARGDIATAAPTQLPWPVNPAAPAPHLARSVAR
                     ARKSLPKREGSTAAAPESTVVPWLDHECDTLRETVALLAATWPGAHAELGEILAQIALL
                     EGPGIDGFTDFAVHGAVFIQRDRLAPAPDGLPGPVRLAEALVHEGTHTRCNAASVAGEP
                     FLRPTPSDAPLVTTPLRLDPRPLNGLFQQVVVLARSVLLYRRLLDTGIDADAGTAGVAA
                     AVRARRDELTRSAEEGVRTLSDHRDALTDHGLSVLADAADVVQVRV"
     gene            5001..6194
                     /locus_tag="P0D74_RS09115"
     CDS             5001..6194
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.3"
                     /locus_tag="P0D74_RS09115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YcaO-like family protein"
                     /protein_id="WP_275462249.1"
                     /sec_met_domain="YcaO (E-value: 9.2e-33, bitscore: 113.3,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAPRWEPQVFAPYPAWPEILFARIAARSPQFDPVCAAGERPVII
                     GSAAGHQREQVVTGARGELVERVSNILAGRAAEAAAAVVATHRDLRRKGIPALDPAELA
                     HRGTSSTPDEARDIQRLWVLGQTVPSGADCYVPAGAVYLHHRPPTGCDPAPGVGSTGVA
                     AHPDPQAAAEHAAWETLERDLLRRSWYGQSPVATLLTPVILPEPLTKLLDGLDVEATAL
                     DVPAPGGTRCVAVCLHAPDRTQQTFGARCGPDDGNLGRLLEKAAYEALMVRWSVDSPVG
                     RATWDSWGGRSAPDSAVRHALWAYHRQDSLGRWTAAAPPESGRRPTAPPDTPGKAGLNA
                     LNDHTGRELVLVDTTHGPARPLEMSVVRIVATGARPLPTRPDGDRDPPVPRGPHPFG"
     gene            6284..7099
                     /locus_tag="P0D74_RS09120"
     CDS             6284..7099
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1248: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024880.3"
                     /locus_tag="P0D74_RS09120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_275462250.1"
                     /transl_table=11
                     /translation="MSRHDSVPPQPTATTADAAPTHGRSPALGAGEPGYADRHATDYDR
                     WFAKPGITPATVDTLAGLAGTGPVLELGIGTGRLALPLAERGLDVHGIEASQAMVHRLR
                     AKPAGDRITVTLGDFADVPVSGTFSLIYIAGGTFFELADRDAKQRCLTAVAAHLAPGGV
                     LALDAHLPEALAVAAASGAPETVSETDDHLILCHRRIDPATQTYQSHYLVHESDRTHHL
                     RVHFHYVSPGELDFMAERAGLRLRARQGTWNQAPFTKDSAYHVSVYEHR"
     gene            7206..8402
                     /locus_tag="P0D74_RS09125"
     CDS             7206..8402
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012296.3"
                     /locus_tag="P0D74_RS09125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytochrome P450"
                     /protein_id="WP_275462251.1"
                     /sec_met_domain="p450 (E-value: 7e-20, bitscore: 70.1,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDPNSRTVDVDFPFPHHDGLGPLPEFDALRRDTPCARIRLPSGD
                     AAWLVTRYADVRTVLADPRFSRTRATRGPGPRFARVTTLPDSILAADPPVHTRLRKLVA
                     PAFTVRRVETMRQQVAQLVDDLLDTVGAADRPADLVPLFTRPLPLAVICDLLGTPRQDG
                     DQLDAWCDALRNLTAVPEAAVTTAVGEMTDYLTALIAAKRRRPADDLLSTLISARDDED
                     RLTQEELLSFSIVLLAGGYGTTADRLAGILHLLLVEPSRYQHLQHAPETIPPAVEELLR
                     YAQTNVQANLRVATEDIELGGVTLRAGDAVMAVNSSANHDTAVFDSPEQLDLARTPNPH
                     LAFGHGAHHCVGAQLARIQLQEALTGLVRRFPTLRATSPPTWKTGLKTRAPRTLPVTW"
     gene            8476..9318
                     /locus_tag="P0D74_RS09130"
     CDS             8476..9318
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012472.3"
                     /locus_tag="P0D74_RS09130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aldo/keto reductase"
                     /protein_id="WP_275462252.1"
                     /transl_table=11
                     /translation="MRTRPFGPTGVRVPVIGQGTWQLEQADPQTAIRTLNTGIDAGMTH
                     LDTAESYGAGAVEELVGQAVRHRRHEVFLASKVRPVRADTRGTVAACEGSLRRLGTDHL
                     DLYLLHWPSQHPLEDTLTGLRQLHRDGKILHYGVSNFYDQHLTDLFAYTEPGEITCNQV
                     LYHLGERSVEHHVATTCLAASSAVVGYSPFGLGNFPPSPQAATVLDRIAHSHAATRRQI
                     ALAFLTRSPHTFAIPRTATPAHALENAGAAQITLTEQDTADLATACPLPADDGHLPVT"
     gene            complement(<9512..9703)
                     /locus_tag="P0D74_RS09135"
                     /pseudo=""
     CDS             complement(<9512..9703)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013001012.1"
                     /locus_tag="P0D74_RS09135"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSLQAAEFFAELCLPFTNGTVVGNTYYATPIQDAPLRLRIRFTPT
                     LYADTYGGLQATVLHADNG"
     gene            complement(9727..10566)
                     /locus_tag="P0D74_RS09140"
     CDS             complement(9727..10566)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015036829.1"
                     /locus_tag="P0D74_RS09140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF317 domain-containing protein"
                     /protein_id="WP_275462253.1"
                     /transl_table=11
                     /translation="MPDRPTTDGDVYVSPRYLAGIGSEPDSSFAPVAHWPHHHLDDGPC
                     QLVISSPDYRIKIGWFGDDYDTWKISAAEDAVSEAHWSARINQNTPPEIVHGLTSALAN
                     HWDEDSETFLKDHSYYWTDAVQPLLDAGWERKPVRRGLVEIVSPDQLAGASIDVVRYGH
                     DAELVTLWAGPPGWGTRAELVFTARTPKHLIAATAAALVDPTPVVRYTEHLHRSLAQRA
                     HLTPIQPPKPPAPTPLDVQRATAGHRLPPAPRTRSVPRWSTTTLPPTTPAAPAGPRR"
ORIGIN
        1 cccgggtcga tccgctccgg gctgtacccc aggtggaagt cctcgccggc cctgagaccg
       61 gactcctctt ccaggatcgc gccgaccagt tcctccgtcg ttcccgggta ggtggtggac
      121 tccaggacga ccacggcccc gggcctgagg caaccgccga gcatccgggc ggaaccggcg
      181 atgtgcgaca ggtcgggcat cctgtcgcac agcggtgtcg gtacggcgat caccgcgagg
      241 tcgaagccgg cgcactcctc ggggtcggcg gtggggcggt aggccccgga ggcgaggagt
      301 tctcggaggt ccgcggcgga gacgtcgggt acgtacggct ctcccgcgct gagttgcttg
      361 acccgtcgtt cgttcacgtc gaagccgacg acgtggtgac cggcccatgc ggctcggatc
      421 gccagtggta gtcccacgta gccctggccg gcgacgacaa cccggctagg ggccgtcaac
      481 gggaaccgac catgatgagg tgttcgcggc cgtgtggggc cggccacacc gccctctgcg
      541 tgcacccgta ctcctctcca ggcgctcgtc tcgtgccgtg gtgatggcgc tcatgacggg
      601 ccggggcgcg ctcggtggca gacacgaacc catggtgtgg gggctccttc ctgcccgcca
      661 gggcacgatc cggccaccag ccggaagttt gcggccgggc tcgtcgcgaa gcacgcctga
      721 ggtgtccggg acgtgtgcac ggttctcagg acagcagggt gaggacgttg tgggtgaggg
      781 tggccacctg cgggtcgagg gacaggaggc gggcccagtc ggtggtggcc gcggtgaaca
      841 ccgtgccgcc cttggtatgc agcccgaagg tcgcggccct ggccgggtcg gggtcctcac
      901 gggcggggac ctgccaaccg gaggccgggc ccaaagggag atcggccacc cccaggatcg
      961 tgaagtccgt gggcgtgccg tccgttccgg tgggtacggc ccgcccgcgg tggtcgcgca
     1021 cgtgggcggc gccgtcgcat tcgtagccca ccagggaatg tgcggcgccg aggatgtcgc
     1081 cgtcactcag tcccgtgccg gcgaacactg ggtgctcggt gcactggacg gtgaagccca
     1141 acggtgggcg gcttccctcc caatatccgc cgccgttgcg gtagctgacg ccggtgagcg
     1201 cgttctcggg tgccgactcc caccagtggt cgggggctcc gggcgtgccg tccacgtcca
     1261 gcgcctcgcg ggctcccggt gggtacttcg cacaccgcgc ggtcgcaccg tcgtcgctca
     1321 cggcgacgcg ccaccaacag gtgttggcgc cgaagaaagc gatgttgccg ccgcggtcgc
     1381 ggaaggccgt gacgttgcgt cgggtgtccg cgctccagta ctcgtcgtgt cctgcggaga
     1441 tcagcaggcg gtagccgtcc gtgaggtgcg gggtttggtg caggtcgagg tcggtgcagt
     1501 actcggcggt gaatccggcg gcctccaacc aggcgatgaa cggggcgtcc cagtgcgcga
     1561 aggtctggcg gggcgtttgg ggggcgtggg cgtccgggtg ccccttgacg gggccgccgg
     1621 ttccgccgcc gggtcgccgc aggctgacgg tgaactcgcc ggtggctttg cctgatgcgg
     1681 acacgggcgg tgaaccgtac agacagccac cgccgctgcg gttgtacgcg tggtaggtga
     1741 agagcgggac cttgtagagg atgcctgtgg tccgtgcggg ttcgcgggcc ctgaccacga
     1801 acagcaggcg ggaacgccgg gcgtccaggg cttcggcgca ggagggtacg ggcgccggtg
     1861 gcgcggcgtc gggccccagg acggcgaggt agacgcccga gggccagtcc tcggggaccg
     1921 ggaagtcgtg gtggggccag tcccaatccg cgtcgtggcg tccgggtggc gcgtggcggc
     1981 ctggcccccg gatgcttccc atgtggcgga tctgggcgcc gcaacggtag aagtcagccc
     2041 ggaaccaggg ggcgtgggtc gagacgtgga ggcgcagggt gtcgccggcc cgcacgctcg
     2101 cgtcggaggg atacccctcg acgaccgccc cagccgaggc tgcgacagcg ctcgacggtg
     2161 ccgtcatgtg gggctcactg actgctcgtc actccggtcg tggaggggaa ggccggcgag
     2221 gacgacgtcg gcccgacgac gccaggtgta gcggtccagg acctggcgtc gtgcctcgtc
     2281 gccgagcgcc tgtcgcagtg cccggtcggc gacgagctcg ctcagcgcgt cggcccatgc
     2341 gtcggcgtcg tccggtgggc gcagcaggca gttgacccgg tcctggagta cctcgcgcag
     2401 cacgggtagg tcggaggcga tcatcgggtg gccgtgcgcc atgtactcga acactttcat
     2461 gggcgaggcc cactgcccgg tctcggagag ctttccccag gtgtagacct tgtgctggta
     2521 gggggcgaga acgacatcga agtgcgggta gtagtagggg agttcggctg gtgggcggtg
     2581 tccgtggaag tgcacgttgg gcaggtcgca ggaggtgtcc cagcggtccc ggtcccccgt
     2641 ggtgccgccg accaggtgga agtcgaagcc gggcagtcgg ccggcgagtt ccacgatgag
     2701 gccgatgccg cgcccttcgt agaggtgacc gacgtagccg atgcgcggca cgttcggacg
     2761 gcccggaagt tccgggacgg cagtggtcgg cacgggttcg acggggtcgg cgccgtcggg
     2821 agccacgagg agcggcaacg tgccggcttc ggcgtgggcc tgttggaagt cgcgcgccag
     2881 cgcgtgggtg atgacgacga cgcgcgacag cagtggttgg gcgagaaggt cgtgttccat
     2941 ctcgggcggt gccaggtcgc tgcgtagctg gtggacctcg tagacgaaag gggccaggtc
     3001 ggcggcccgg gagaggctgt agggctcacg cccgtacacg aggtcaggga ggtcgcggat
     3061 cagcaggtcc cgtacgcggt cggcgcgctt ccagtagccg tgcggggtgt agtccggtgc
     3121 ggacaccaga cggatcggga accggtggcg cacgccgtag taggcgtacg ggtcagcggc
     3181 gagctgcggt ccgggcaggg agtacagggt gacgtcgtgt ccggcggcgc tgaacgcgtc
     3241 gcacatccgc atgacatgga cgccgttggc gaaggtggac ggcatgctgc cgccgtgcag
     3301 gtaggcgatc cgcatgccgg cctaaccgtg gagttgcgtg gtcgtcggcg ggtggggccg
     3361 gtcagcggcc ggtagccgtc gggacgtgcc gtcgagtggt ccttgcaccg tcgtctcctc
     3421 agctcaacgt gggcgggcgt ggtggagggg ctcggggtag gtggggcgga cggaccggca
     3481 cttgccgtga tctcggttcg ggaactccga tcggtgccac gacgggtcgt ccgaggcgag
     3541 ttgcctgaca tgagttgcac gaggcgacct gaggcgagtg gcctcagagg gagtcatcat
     3601 cgggggtgtc ttcttgacgg gcaaagagtg cggagttggc aaggggcggg tttcggcccc
     3661 tgcccaagtc gggcgtccgc acgcctggtt ccaccgtgtg accagttcgg gggagctcag
     3721 tcgagcacgg cccgacggcg ccgaacacgt gccgggcgat gacgtgatta cttcttgtga
     3781 tcacttgatc acgctcttag gtttatgttg ccggactgcg cccaactgcg gtgcagccgg
     3841 ccatccgaag ggagaccgcg atggaggaca tcgagcagct ttcggaagaa gaactgcccg
     3901 agctggcgtt gatgatcgac gtacccacca actgacgcct cttcatctca actcgggtgg
     3961 cgggccgggc tttccggtcc gcccccgtcc acccccgggc agacggaggt agtcacggtg
     4021 cgcgtggaac ccgacgcaca gcaaagcgcc gaggaacgca cggccctggc ccgcaccacc
     4081 cgatccgccc tggcacgggc cgaactgcac gtgaccccgg cggaggcgac acaccccgcc
     4141 atcatcgaaa tcgcccacct cgcccaacac gtcgggcgag ccgattcgcc cgaccgtgag
     4201 cggctcgaca ggctcggccg cgcgctcgac cgggcccgcg gtgacatcgc gaccgcagca
     4261 ccgacgcagt tgccctggcc ggtcaaccct gccgcgcccg ccccgcacct ggcacgcagc
     4321 gtcgcccgag ctcggaaatc cctaccgaag cgggaaggct ccactgcggc tgcgccggag
     4381 tccaccgttg tcccctggct cgatcatgaa tgcgacaccc tgcgcgagac cgtggcgctg
     4441 ctcgccgcca cctggcccgg tgctcatgca gaacttggtg aaatcctcgc ccagatagcc
     4501 ttgttggaag gtcccggcat cgacggcttc accgacttcg cggtgcacgg tgccgtgttc
     4561 atccagcgcg accgcctggc accggcgccc gacgggctgc cagggcccgt ccgtctggcc
     4621 gaggcactcg tccacgaggg cacccacacc cgatgcaacg cggcatccgt ggccggtgag
     4681 cccttcctgc ggcccacgcc gagcgacgcg ccgctcgtca ccaccccgct ccgcctcgac
     4741 ccccgcccct tgaacggact gttccaacag gtcgtggtct tggcccgcag cgtactgctg
     4801 taccgacgac tgctcgacac tgggatcgac gccgacgcag gcaccgccgg cgtggccgct
     4861 gccgtacgcg cacgccgcga cgagttgacc cgctccgcgg aagaaggggt gcgcaccctg
     4921 tcggaccacc gggacgcgct caccgaccac ggactctccg tcctggcgga cgccgccgac
     4981 gtggtccagg tgcgcgtttg atggccgcac cgcgctggga gccccaggtc ttcgccccct
     5041 accccgcgtg gcccgagatc ctcttcgccc gtatcgcggc acgctcaccg cagttcgatc
     5101 cggtgtgcgc cgccggcgag cggcccgtca tcatcggcag cgcggccggg caccagcggg
     5161 agcaggtcgt caccggagcc cgcggtgaac tcgtcgaacg ggtcagcaac atcctggccg
     5221 gtcgcgccgc cgaagccgcc gctgcggtcg tggcgaccca tcgcgacctg cgccgcaagg
     5281 ggatacccgc cctcgacccc gccgaactcg cccacagagg taccagcagc acccccgacg
     5341 aggcccgcga catccagcgg ttgtgggtgt tgggccagac agtccccagc ggcgccgact
     5401 gctacgtacc ggccggtgcc gtctacctcc atcaccgacc cccgacaggc tgcgacccgg
     5461 cccccggcgt cggctccacc ggcgtcgccg cgcaccccga tccccaggca gccgcggaac
     5521 acgccgcctg ggaaactctc gaacgcgatc tccttcgcag aagctggtac gggcagtcac
     5581 ccgtcgccac cctgctcaca ccggtgatcc tgcccgagcc gctcaccaaa ctcctggacg
     5641 ggctcgatgt cgaggccacc gccctcgacg tccccgcccc cggagggacc cggtgcgtgg
     5701 ctgtctgcct gcacgcaccc gaccggacgc aacagacgtt cggcgcccgg tgcggacccg
     5761 acgacgggaa cctcgggaga ctgttggaga aggccgccta cgaggcgttg atggttcgtt
     5821 ggagcgtcga ctccccggtg ggccgcgcca cgtgggacag ctgggggggt cgaagtgcgc
     5881 ccgactcggc agtccggcac gcgctgtggg cctaccaccg gcaggacagc ctgggccggt
     5941 ggaccgcagc ggctccaccg gagagcggac gtcggcccac cgcgccgcca gacactccgg
     6001 gcaaggccgg cctcaacgcg ctgaacgacc acaccggccg ggaactcgtc ctcgtcgaca
     6061 cgacgcacgg gccggcccgg cccctggaaa tgagcgtggt gcggatcgtc gcgaccggag
     6121 cccgtcccct gcccacgcgc ccggacggcg accgcgaccc accggtccca cgagggccgc
     6181 acccctttgg ctgagcatcg cccgcccagc caccccaacc gcaccgcgtg gcccggactc
     6241 cggccgcagt gcggcccgac cacaggccga ggagacctcg accatgtcca gacacgacag
     6301 cgtcccgccc cagccgacag cgacgacggc cgacgccgcc ccgacccacg gccgctcgcc
     6361 cgccctcggg gcgggcgagc cggggtacgc cgacaggcac gcgaccgact acgaccgctg
     6421 gttcgccaaa cccggcatca cgccggccac ggtggacacc ctggcgggac tcgccggtac
     6481 cgggccggtg ctcgaactcg gcatcggcac cggcagactc gcactccccc tggcggagcg
     6541 cggcctcgac gtccacggga tcgaggcgtc gcaagcaatg gtccaccgcc tgcgcgccaa
     6601 acccgctggc gaccgcatca ccgtcacgct cggcgacttc gcggacgtac cggtctccgg
     6661 aacgttctcc ctgatctaca ttgccggggg caccttcttc gaactcgcgg accgggatgc
     6721 caagcaacgc tgcctcaccg cggtcgccgc ccacctggcg cccggcggcg tcctcgccct
     6781 cgatgcccac ctacccgaag cactcgcagt cgccgccgcg tccggcgcgc ccgagaccgt
     6841 gtccgagacc gacgaccacc tcatcctgtg tcatcgccgg atcgatcccg ccacccagac
     6901 ctatcagtcc cactacctcg tccacgagag cgaccggacc caccacctcc gcgtccactt
     6961 ccactacgtg tcgcccggcg aactcgactt catggccgag cgggccggac ttcgcctgag
     7021 ggcacgtcaa ggaacatgga atcaggcgcc gttcacgaaa gacagcgcct accacgtctc
     7081 cgtctacgag caccgctgag agctcaggcc cccagttcgg acagccccac ccacccggtg
     7141 cccgcccctc accgcccgtc ccgaccctcc ccgcggccac gaccgcacac cgtcccggag
     7201 cactcgtgac agacccgaac agccgaaccg tcgacgtcga tttccccttc ccccaccacg
     7261 acggactcgg tcccctcccg gagttcgacg ccctgcgtcg cgacacgccc tgtgcccgca
     7321 tccgcctgcc cagcggagac gccgcctggc tggtcacccg ctacgcggac gtccgcaccg
     7381 tactggccga cccgcgcttc agccgcacgc gcgcgacccg aggcccgggg ccacgcttcg
     7441 cccgcgtgac caccctgccc gactcgatcc tggccgccga cccacccgtc cacacccgac
     7501 tacgcaagct cgtcgcccca gccttcaccg tccgtcgcgt cgagaccatg cggcagcagg
     7561 tggcccaact cgtcgacgac ctcctcgaca cggtcggcgc ggccgaccgt ccagccgacc
     7621 tcgtccccct cttcacccgg ccattgcccc tggccgtcat ctgcgacctg ctcggcacac
     7681 cgcgccagga cggcgaccaa ctcgacgcgt ggtgcgacgc actgcgcaac ctcaccgcgg
     7741 tccccgaggc cgccgtcacc acggcggtcg gcgagatgac cgactacctc accgcgctca
     7801 tcgcagccaa acgccgccgg cccgccgacg acctcctgag caccctgatc tccgcacgtg
     7861 acgacgagga ccggctcacc caggaggaac tgctctcctt cagcatcgtc ctgctcgccg
     7921 gcggctacgg caccacagcc gaccgcctcg ccggcatcct ccacctcctg ctcgtggaac
     7981 ccagccgcta ccaacacctc caacacgccc cggagacgat ccccccggcc gtcgaggaac
     8041 tcctgcgcta tgcccagacc aacgtgcagg ccaacctgcg ggtcgccacc gaagacatcg
     8101 aactcggcgg cgtcaccctc cgcgcgggcg acgccgtgat ggccgtcaac tcctccgcca
     8161 accatgacac cgccgtcttc gattcccccg aacaactcga cctcgcccgc accccgaacc
     8221 cccatctggc cttcggccac ggtgcccacc actgcgtagg cgcacaactc gcccggatcc
     8281 aactacagga ggcgcttacg ggactcgtcc gccgcttccc cacactccgg gccacctcac
     8341 cccccacctg gaagacgggg ttgaagaccc gcgcaccccg cacgctaccg gtgacctggt
     8401 gagacacccg tggctcccag tcacgccagc cgtccccacc cctgaccgtt cggacgtccc
     8461 cggaggcagc cccccatgcg cacccgcccg ttcggtccca ccggtgtccg agtccccgtc
     8521 atcggccagg gcacctggca gctggaacag gccgaccctc agaccgccat ccgtaccctc
     8581 aacaccggta tcgacgccgg aatgacacac ctcgacaccg ccgagtccta cggagccggc
     8641 gccgtcgagg aactcgtcgg ccaagccgtg cgccaccggc gccacgaggt gttcctcgcc
     8701 tccaaggtcc ggcccgtccg cgccgacacc cgcggaaccg tggccgcctg cgaaggcagc
     8761 ctgcgccgcc tcggcaccga ccacctggac ctctatctcc tgcactggcc cagccagcac
     8821 cccctggaag acaccctcac cggcctgcgt cagctccacc gagacggcaa gatcctccac
     8881 tacggcgtca gcaacttcta cgaccagcac ctcaccgacc tcttcgccta taccgaaccc
     8941 ggcgagatca cctgcaacca ggtcctctac cacctcggcg aacgctccgt cgaacaccac
     9001 gtcgccacca cctgcctggc agccagcagt gccgtggtcg gctacagccc cttcggcctg
     9061 ggcaacttcc caccctcacc acaagccgcc accgtcctcg accggatcgc ccactcccac
     9121 gccgccaccc gccgccagat cgccctcgcc ttcctcaccc gctccccgca caccttcgcc
     9181 atccccagga ccgccacccc cgcacacgcc ctggaaaacg ccggagcagc acagatcacc
     9241 ctcaccgaac aggacaccgc cgacctcgca acggcctgcc ccctccctgc cgatgatgga
     9301 catctccccg tcacctgaga cagcgcacac cacgtccatc gccggctgat tcaaagtcat
     9361 cccagcccgt gcctcgtacg gcgcccgacc atcactcatc gcaacgagcg cacccacctt
     9421 cgccggacag ggtcaacgcg cgcgtagctg cagcggcagg cgacggccag ccgggggcga
     9481 gcgcatacaa ctcgggatgc gaggcggtga ccccgttgtc tgcgtgaagc accgttgctt
     9541 gcaggccgcc gtaggtgtcc gcgtagaggg tcggggtgaa gcggatgcgc aggcgcagcg
     9601 gtgcgtcctg gatgggggtg gcgtagtagg tgttgcccac gacggtgccg ttagtgaagg
     9661 ggaggcacag ctcggcgaag aactcggcgg cttgcaggga cacgttcctc cggaggtcga
     9721 gcggggttat cggcgtgggc cggcgggtgc tgcgggggtg gtgggtggga gcgtggtggt
     9781 gctccagcgg ggcacgctgc gcgtgcgcgg tgcgggtggg aggcggtggc ccgcggtggc
     9841 gcgttgaacg tcgagcgggg tgggggccgg gggtttcggc ggctggatgg gtgtgaggtg
     9901 ggcccgttgc gcgaggctgc ggtggaggtg ctccgtgtag cggacgacgg gggtcgggtc
     9961 gaccagcgcg gcggcggtgg cggcgatcaa gtgcttcggg gtcctggcgg tgaagacgag
    10021 ttcggcgcgg gtgccccagc cgggtggtcc ggcccacaag gtgaccagct cggcgtcgtg
    10081 gccgtagcgg accacgtcga tgctggcgcc ggccagttgg tctggggaga cgatctccac
    10141 caggccgcgg cgtaccggtt tgcgttccca gcccgcgtcg agcagcggct gtaccgcgtc
    10201 ggtccagtag taggagtggt ccttgaggaa ggtctcgctg tcctcgtccc agtggttggc
    10261 gagggcgctg gtgaggccgt ggacgatctc ggggggcgtg ttctggttga tgcgcgcact
    10321 ccagtgagcc tcggagacgg cgtcctcggc ggcgctgatc ttccatgtgt cgtagtcatc
    10381 gccgaaccag ccgatcttga tgcggtagtc cggcgaggag atgaccagct ggcagggccc
    10441 gtcgtcgagg tggtggtgcg gccagtgggc gacaggggcg aagctgctgt ccggctcgga
    10501 tccgatcccg gcgaggtagc gcggggagac gtagacgtct ccgtcggtgg tggggcggtc
    10561 aggcatgtgt gtccggaagg tcgcgggcgg gcgaggccgg ctcgacgcgg acgctgaggt
    10621 ggccgagtgc ggtctcgatg cgcgtggcgg cggccgtggc ggtgggggcg gtggtgatga
    10681 ggtagccgat gcgggcggtg aagggatcgc ccccctcggc ggggagcagg agtcggtcgc
    10741 ccagggcgcg gtggaagtgg gcatgctcca gccacggtgg gtgcgagccg tcgtggtgga
    10801 ggtgggtcag gaggtgtccg agctgtgctg gtggcacgac ggcgacgagc agtccggcgg
    10861 cggggaaggg cgccaggagc agcaggatcg tgagggcgag ggtgctgccg aagacgtcgg
    10921 gcgggatgag gtgggagcgc agcgtgcgta ggacgaccgt cagtccgcct tcgccggcca
    10981 tcaccacggc gatgagcagg aggtaggtgc ggtaggtgtc ggcctgcgag agggcgaggg
    11041 ttgcggtggc ggcgagtgcg gcggagacgg cgccgaccgg ccacagtccc cagcggtcgt
    11101 tggcgcggcg ggcgacggtg atggcgagca gcgaggcgat ggctgcggcg gaccagatca
    11161 gcccggcctc cgcgcttgag tgtcccaggc ggtt
//
