LOCUS       NZ_CP119144            10981 bp    DNA     linear   CON 22-NOV-2023
DEFINITION  Streptomyces noursei strain C27 chromosome, complete genome.
ACCESSION   NZ_CP119144
VERSION     NZ_CP119144.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces noursei
  ORGANISM  Streptomyces noursei
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9627936)
  AUTHORS   Go,B. and Lu,Y.
  TITLE     Control effect and mechanism of Streptomyces NK27 against gray mold
            of grape
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9627936)
  AUTHORS   Go,B. and Lu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-FEB-2023) State Key Laboratory for Biology of Plant
            Diseases and Insect Pests, Institute of Plant Protection, Chinese
            Academy of Agricultural Sciences, Yuanmingyuan, Beijing 100193,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-OCT-2021
            Assembly Method                   :: ABySS v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 98x
            Sequencing Technology             :: Complete Genomics
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 14:00:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,442
            CDSs (total)                      :: 8,348
            Genes (coding)                    :: 8,203
            CDSs (with protein)               :: 8,203
            Genes (RNA)                       :: 94
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 46 of 145
            Pseudo Genes (incomplete)         :: 121 of 145
            Pseudo Genes (internal stop)      :: 12 of 145
            Pseudo Genes (multiple problems)  :: 30 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:17:19
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1080964
            Orig. end                         :: 1091945
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP119144.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10981
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="10"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10981
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10981
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5981](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5981
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(92..109)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.36"
                     /tool="antismash"
     misc_feature    complement(103..120)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.67"
                     /tool="antismash"
     misc_feature    404..420
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 22.72"
                     /tool="antismash"
     gene            complement(963..3389)
                     /locus_tag="P0D74_RS04415"
     CDS             complement(963..3389)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574653.1"
                     /locus_tag="P0D74_RS04415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_275461452.1"
                     /transl_table=11
                     /translation="MTQTTTDQLPVRTADSAAGPPPAPLPASGHRRARAGLVPVLAFAG
                     IVVAVMQTLLVPVIKDLPTLLGTASSNATWVMTATLLAGAVATPIMGRLGDLYGKRRML
                     LVSLAVMVIGSLICGFTSQLVIMIVGRALQGFAMGAIPLGIGLMRDELPRERLGSAMAL
                     MSSSIGVGGGLALPVAALIAQHADWHTLFFAAAGLGVLSMLLTLFAVPETTVRAPGRFD
                     IAGAVGLSAGLVALLLPITKGSDWGWASPRTLVLLGVALVILVLWGVMELRIADPLVDL
                     RTSARREVLLTNLASITVGVAFYAISLVLPQLLQLPKSTGYGLGQSMVVAGLCVAPLGL
                     TMMVVAPVYARIAARRGPKVSLLLGMLIIGVGYGGGIGLMQAPWQTVIIAVVVGAGIGL
                     AYSSLPALIIGAVDPSETGAANGLNTLMRSIGTSVSSAVIGMVLAHMSEPLGPVAVPTM
                     AGFRVSFLIATAAVVLGVVVAAFLPSQRKATATRVAQSAEGAPAAGQESTAARPTAPSP
                     GAVASEEPRAAGAPVAEQTEAAEEVGAGFRGRVVDSAGGPVAGATVTLIDRHGQQAGVT
                     TAAADGSYALTAPTAGTYVLTGAAPGHTPQAASATHYGEGTVAEVRLALVPTGSRLRGT
                     LRGGPDGAALAGAGVVVTDGRGDVVTCTSTDADGHWEVGQLPDGDYTLVLSAAGHQPEA
                     RAVQLTGADGADSPNRPGTQDVRLRPTATVRGTVRGPDGRPLADAAVTLLEDGTVTGHT
                     VTGADGAYAFADLKGRQYTLSAAGYPPHAEPISLTGGADERLDLDLAQPGAAGGR"
     misc_feature    3548..3563
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.69"
                     /tool="antismash"
     gene            complement(4139..4825)
                     /locus_tag="P0D74_RS04420"
     CDS             complement(4139..4825)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092050.1"
                     /locus_tag="P0D74_RS04420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_275461453.1"
                     /transl_table=11
                     /translation="MAQQDRAIRTRRMILEAAASVFDDRGYDRATIAEVLERAGVTKGA
                     LYFHFASKEQLALAVLNEHVLDIAVETQQIRLQEFVDAGQVLAYRLRSDPIQRGAARLA
                     VEQGSTHLDRQQSMLSWTRFVEGLLSEARERGEIMDSVNVRETAELFVGAFAGIQMMSH
                     ALTNRADLGHRLAVFFEHTLPSIAVPAVLAKLKLDPERGEKIDAALQEKAVSSAQPQGD
                     GEAVAS"
     misc_feature    4926..4939
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.3"
                     /tool="antismash"
     misc_feature    4927..4940
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.86"
                     /tool="antismash"
     gene            5001..5981
                     /locus_tag="P0D74_RS04425"
     CDS             5001..5981
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608008.1"
                     /locus_tag="P0D74_RS04425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_275461454.1"
                     /sec_met_domain="AfsA (E-value: 1.1e-16, bitscore: 59.6,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDTMHVSGAVQTRAPHQLRPDQDEPTAQRFRTDAPVPCEYVHRP
                     VVDDILVTSWSRVDDCHFSVTAHWPRDHGYFAPRRGRDSLILTGETIRQAGLLLSHTEF
                     NVPVGHHFILGDLVYTMRPEHLPADGGPTTLTIDVACSSLRTRRTTLASGRFDMTMRAA
                     DQVVVVGHSHVTVASPAVYRRIRGEHLTAQRDVGPLPSPVAPQLTGSDTEHDVLLSPTN
                     RPGRWHLRIAPGHAAVVNPANDHVPGMVLVDAAQQAAHALTAPERFAPHTFDMQFSRYA
                     EHGTPCVIEARRVASAPGTATVRVTGVQDGEPVFLCTLTAQDERG"
     gene            6197..6961
                     /locus_tag="P0D74_RS04430"
     CDS             6197..6961
                     /NRPS_PKS="Domain: PKS_KR (7-185). E-value: 1.1e-08. Score:
                     27.1. Matches aSDomain:
                     nrpspksdomains_P0D74_RS04430_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608009.1"
                     /locus_tag="P0D74_RS04430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_275461455.1"
                     /transl_table=11
                     /translation="MGDLNGKTALVTGSSRGIGRGIARRLAREGALVAVHYNTDDVAAK
                     ETLDGIKAEGGRAFVIGAELGVPGDAAALFAAFDAELAAYGAEPGLDILVNNAATRFSG
                     HIGETTEDEFDRTVAINLKAPFFIVQHALHRMRDGGRIINISSAVTSTAYPSSIAYSVS
                     KGGIDTLTRTLAKDLGARGITVNALAPGWVETHVTAARTAVPEQRAALAAYSVFNRIGQ
                     PTDIADVVAFLASDDSRWITGQRIDVTGGSML"
     aSDomain        6218..6751
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_P0D74_RS04430_PKS_KR.1"
                     /evalue="1.10E-08"
                     /label="P0D74_RS04430_PKS_KR.1"
                     /locus_tag="P0D74_RS04430"
                     /protein_end="185"
                     /protein_start="7"
                     /score="27.1"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TALVTGSSRGIGRGIARRLAREGALVAVHYNTDDVAAKETLDGIK
                     AEGGRAFVIGAELGVPGDAAALFAAFDAELAAYGAEPGLDILVNNAATRFSGHIGETTE
                     DEFDRTVAINLKAPFFIVQHALHRMRDGGRIINISSAVTSTAYPSSIAYSVSKGGIDTL
                     TRTLAKDLGARGITV"
     CDS_motif       6224..6289
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_P0D74_RS04430_0001"
                     /evalue="2.30E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="P0D74_RS04430"
                     /protein_end="31"
                     /protein_start="9"
                     /score="13.8"
                     /tool="antismash"
                     /translation="LVTGSSRGIGRGIARRLAREGA"
     gene            complement(7020..7529)
                     /locus_tag="P0D74_RS04435"
     CDS             complement(7020..7529)
                     /EC_number="2.1.1.63"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574649.1"
                     /locus_tag="P0D74_RS04435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="WP_275461456.1"
                     /transl_table=11
                     /translation="MTLYATLDSPLGELLLVGERSATAPGGTALASLSVPDQKRGAVVQ
                     EGWTHDPAAFAEIARQLRGYFDGATAPFTVECVPRGTEFQQRVWTALEAIPRGTTTTYG
                     AIATAIGAPRGAVRAIGTAIGANPLLVVRPCHRVIGADGSLTGYAGGLARKRLLLDLEG
                     VAADAA"
     gene            complement(7526..8995)
                     /locus_tag="P0D74_RS04440"
     CDS             complement(7526..8995)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574648.1"
                     /locus_tag="P0D74_RS04440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DNA-3-methyladenine glycosylase 2 family protein"
                     /protein_id="WP_275461457.1"
                     /transl_table=11
                     /translation="MSEDSRYEAVRSRDARFDGAFFFAVSTTGIYCRPSCPATTPRRRN
                     VTFFPTAAAAQGSGYRACRRCRPDAVPGSAEWNARADVVGRAVRLIGDGVVDREGVAGL
                     ASRLGYSARQLQRQLTAELGAGPTALARAQRAHTARVLLQTTALPIAEIAFAAGFASVR
                     QFNDTIKEVYALTPSGLRAVRPGARSGPTPPSGTPGVLPLRLAFRGPYAARQVFDQLQA
                     QALGGIEEVTGAPGRRTYRRTLRLPNGTGIMEVDEVIGDGWLDCRLHLTELRDLTTATQ
                     RVRRLFDLDADPYAVGERLGADPVLAPLVARCPGLRSPGTVDPVELAVRAVLARRTGPA
                     AGRDLGERLVAGYGEPLPRPVGGLTHLFPRAAELARAPLAGLAPSPSRAAALRALAAAL
                     AARDVVLDAGTDRDRAERALRALPGIGPWTAGYVRMRALGDPDVLLGTPPPGTTPALWS
                     PWRSYATHYLHAADPAGTPARHSPQEERIDA"
     gene            complement(9119..9346)
                     /locus_tag="P0D74_RS04445"
     CDS             complement(9119..9346)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608014.1"
                     /locus_tag="P0D74_RS04445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_275461458.1"
                     /transl_table=11
                     /translation="MSEHPPVIVHPPTPSGGRRVTVHGQIVGLAHGRGDVAEFLRRAGV
                     ADPAHDVSLDDPRLIEWRGGSLDDWPMPPA"
     gene            9529..10773
                     /locus_tag="P0D74_RS04450"
     CDS             9529..10773
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576760.1"
                     /locus_tag="P0D74_RS04450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytochrome P450"
                     /protein_id="WP_275467480.1"
                     /sec_met_domain="p450 (E-value: 4.2e-19, bitscore: 67.5,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MESAEFSRDPYPLLAALRARGPVQRVRTGKDRTTWVVTGWAEARA
                     ALADRRLSKDTARYFADRPSGRDLAPAVSQSMLATDPPDHGRLRKLAVAAFTPAAVGRL
                     EPRIREIAEGLAAELGRSAGAAGGPVDLVEGFAVPLPIAVICELLAVPEADRAAVRRWS
                     DDLFAAADPDTTDRASHALAGYVAELIAARRAAPGDDVLSALIAARDAGDRLSERELVS
                     LAVLLVVAGHETTTHLIGNGTLALLRDDALRARLRDDPGLLPAAVEEFLRYEAPVTLAT
                     FRYATEAFDLGGVRIGAGDVVLVSPGGANRDPARFDEPDVVRLARSGAGGHLSFGHGPH
                     HCLGAPLARAEARIAFEVLLARFPGLRLAAGGGRGAGGSDGADGVRWRRTRLMRGPAQL
                     PVLLGPLREAPEGRG"
     misc_feature    10846..10859
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.8"
                     /tool="antismash"
     misc_feature    complement(10847..10860)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.59"
                     /tool="antismash"
     misc_feature    complement(10851..10866)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.53"
                     /tool="antismash"
ORIGIN
        1 cttgtctggg gttggtgggt accctcgtat cagtgggtgc gtacggtccg tccgttgctc
       61 ttccagcgtg ctcccgggca ctgccggtcc gctaacgagg cactaacacc ccgctaacga
      121 ggccgcacgc ccggctcgac gctgctccgg cgcccggtgt gaccaggcgg aactccggtg
      181 cgccgcccag gctcgcagaa atccgcgcgg ttgcgggcgc ggcactacga ggttcggccg
      241 tttcagcctt cctgctcacg ggcctcagaa aacccatgaa ttccccggcg ccccacctcg
      301 cggtcccatg cccctggtaa gtgcgcaggg ccgatccccg agcgccggtg ccgagggagt
      361 cgctgcgcgc ccggggcgga ccgggggtcc gcactcgacc gaaatcccac catagggtgt
      421 ggacgagacg ctctcgttga cgccggggtt gcacggcgac ggcactttcc ggccatgggc
      481 caccagtcac ccggctgcgc tgcctcggcg ccgcgccgtc cgcaactgac ggcgtttccg
      541 gaccggcccc ctacggtggc actcctccgc tcccctcgat ggcggccggg cacgcgggag
      601 tggcgcgggc cgttaccggg ggccggggcg gaggcggcgg ggaggcgtcg agcgcggagg
      661 tggcggggca gcggcccggg cgtaggcgcg gaggctcgcc agggggttat gtgcgcgggc
      721 cgacggttac cgcaggccga cgcccgccgg gcggagcccc accgccgagg gccggatctc
      781 aacccctccc ggggtcttcc acctctcaac cgacctgcga aaaagcggag ttgacgactc
      841 cccccatgac aggcagcctc ccccggccgc cggtcaggga cgacgccccg tcggccgcgg
      901 cgggcggggc cgacgggacc gacggggaag ggcgtcgcgg tgggggtcgt cgcggtgggg
      961 cgtcagcgcc cgccggcggc acccggttgg gccaagtcga ggtccagccg ctcgtcggca
     1021 ccgccagtca gggatatggg ctccgcgtgc ggcggatacc cggccgccga cagcgtgtac
     1081 tgacggccct tcaggtctgc gaaggcgtag gcgccgtccg cgccggtgac ggtgtggccg
     1141 gtgaccgtgc cgtcctccag cagcgtcacg gcggcatccg ccagcggccg cccgtcgggc
     1201 ccgcggacgg taccgcgcac cgtggcggtc ggccgcaacc gcacgtcctg ggtgcccggc
     1261 cggttcgggg agtccgcccc gtcagcgccg gtcaactgca ccgcacgggc ctccggctgg
     1321 tgccccgcgg cgctgaggac gagcgtgtag tcgccgtccg gcaactggcc cacctcccag
     1381 tgcccgtcgg cgtcggtcga cgtgcaggtc accacgtcgc cccggccgtc ggtgaccacc
     1441 acgccggcgc cggccagcgc cgcgccgtcc ggaccgcccc gcagggtgcc gcggagccgg
     1501 ctaccggtgg gcaccagggc cagtcgcacc tcggcgaccg tgccctcgcc gtagtgcgtg
     1561 gccgaggcgg cctgcggggt gtggcccggg gcggcgccgg tgaggacgta cgtgccggcg
     1621 gtcggggcgg tcagcgcata gctgccgtcg gccgcggccg tggtgacccc ggcctgctgc
     1681 ccgtgccggt cgatcaacgt gaccgtggcg cccgcgaccg gcccacccgc ggaatcgacg
     1741 acccgcccgc ggaaacccgc accgacctcc tcggccgctt cggtctgctc cgcgaccggc
     1801 gccccggccg cgcgcggctc ctcggacgcg accgcacccg gggaaggagc cgtcggccgg
     1861 gccgccgtcg actcctggcc ggcggccggg gcgccctccg cgctctgcgc cacccgcgtg
     1921 gcggtcgcct tccgctggga cggcaggaag gccgccacca ccacgccgag cacgaccgcg
     1981 gctgtggcga tcaggaacga gacccggaac ccggccatcg tgggcaccgc caccggcccc
     2041 agcggctcgg acatgtgggc cagcaccatg ccgatcacgg cgctggacac ggaggtgccg
     2101 atcgaacgca tcagggtgtt caggccgttg gccgcgccgg tctcggacgg gtcgacggcc
     2161 ccgatgatca gcgccggcag cgaggagtac gccaggccga tacccgcgcc gaccaccacc
     2221 gcgatgatca cggtctgcca gggggcctgc atcaggccga tcccgccgcc gtagccgacg
     2281 ccgatgatca gcatgccgag caacagcgag accttggggc cgcggcgggc cgcgatccgg
     2341 gcgtacaccg gcgccacgac catcatcgtc agccccagcg gtgccacgca cagcccggcg
     2401 accaccatcg actgaccgag gccgtaaccg gtcgacttgg gtagttggag cagctgcggc
     2461 agcaccagcg agatcgcgta gaacgccact ccgacggtga tggaggcgag gttggtgagc
     2521 agcacctcgc gccgggcact ggtgcgcagg tcgaccagcg gatcggcgat ccgcagctcc
     2581 atcacgcccc acaggaccag gatcaccaga gcgaccccga gcaggacgag ggtgcgcggc
     2641 gatgcccaac cccagtcgct gcccttggtg atcggcagca ggagggcgac gagtccggcg
     2701 gacaggccga cggcgccggc gatgtcgaag cgtccgggcg cccgcaccgt ggtctccgga
     2761 acggcgaaga gggtgagcag catcgacagc acaccgagcc cggcggcggc gaagaacagc
     2821 gtgtgccagt cggcgtgttg ggcgatcaac gccgccacgg gcagcgccag accaccgccg
     2881 acgccgatcg aggagctcat cagggccatg gccgagccga gccgctcccg gggcagttcg
     2941 tcacgcatca gcccgatgcc gaggggtatc gcgcccatcg cgaagccctg gagcgcccgg
     3001 ccgacgatca tgatgaccaa ctggctggtg aatccgcaga tcagggagcc gatgaccatg
     3061 acagccaggc tcaccagcag catccggcgc ttgccgtaga ggtcaccgag gcgccccatg
     3121 atgggggtgg cgacggcccc ggcgaggagg gtggccgtca tgacccaggt ggcgttgctg
     3181 gaggcggtgc cgagcagcgt cggcaggtcc ttgatgaccg gcaccagcag cgtctgcatc
     3241 accgcgacga cgatgccggc gaaggcgagg acggggacga ggccggcgcg cgctctgcgg
     3301 tgcccgctcg cggggagcgg ggcgggcggc ggcccggcgg cggagtccgc cgtcctcacg
     3361 ggaagctggt cggtcgtcgt ctgggtcatg cgggcggcct ccagggcggc aggagcaagc
     3421 gaaagggtgc gccgtgcgct gcggtgcgcg ctacgcgggc aggggaatac accccctgat
     3481 acgtgcatga cgaacgtttc aacgcaaggg gctattccag gccagcgggc cgtctcgcac
     3541 cgtgggcagt gatctccctc acgcccctcg ggcatcgccc cggtctgcgc caactgccgt
     3601 acgccgcacg accgatcacc ccctgcaact ccagagccgt accggcccgc gcgcccctcg
     3661 atccgcgccc gcctccgctc accgtcccag ccatcgactc tcccccggcc tcgtcactga
     3721 cactggatcc agtgaagcgc acgccactga caatcggtcg cccgagggat caagagcggg
     3781 tcaagagcgg agcgcaccca ctgacggcac gccggtatgg aaccgacgta ccggtgcatc
     3841 gggggatcta ccgccccggg cccggcacaa ccctccgcgc cgccgcatcg cgcacagtac
     3901 gtccacagca acccggttcg acccgattcc gagcaccgat cccccgagcc gcacgacgcc
     3961 gacggcacag tccgggaagg ctccgcgcgc aaggtcgggc gccgcccgcc acgacatgag
     4021 ggatttcccg ccggggtcat cccggacaac cactgaggat tcacccttca acaccgcgtg
     4081 cgcggtatat ttcagccccg catgaggccc gtggccagga tcccgggccg ggggtcggtc
     4141 agctcgccac ggcctcgcca tcgccctggg gctgcgcact cgacacggcc ttctcctgca
     4201 atgcggcgtc gatcttctcc ccgcgctcgg gatccagttt gagcttggcg agcaccgcgg
     4261 gcaccgcgat gctcggcagc gtgtgctcga agaagacggc caaccgatgg ccgaggtcgg
     4321 cacggttggt cagcgcgtgg gacatcattt ggatccctgc gaacgcaccg acgaacagtt
     4381 cggcggtttc acgcacgttc acgctgtcca tgatttcgcc gcgctcccgc gcctcgctca
     4441 gcaacccctc gacgaaccgg gtccaggaaa gcatggactg ctggcgatcg aggtgggtgg
     4501 agccctgctc gacggccagc cgggccgccc cgcgctggat cgggtcgctg cgcaggcggt
     4561 acgcgagcac ctgccccgca tcgacgaatt cctgcagtct gatctgttgc gtctccacgg
     4621 caatgtccag cacgtgctca ttcagcacgg cgagcgccaa ctgctccttg gaagcgaagt
     4681 ggaaatacag ggcgcccttc gtcacgccgg cgcgttcaag aacctcggcg atggtcgcgc
     4741 gatcgtaccc tcggtcgtcg aagaccgatg ccgccgcctc caagatcatt cgccgggtcc
     4801 gaatcgcgcg gtcctgctgt gccacaggaa cgcctcctgt ctctcgaatt cctcaagtgt
     4861 gccaaccgct cgctggagct caagtctccg cccggtcagc ctatttcctc tttccgtcag
     4921 cccggcattg aaaataaaac cggatggtac gtatcttatc agcgacgcac ctcaacctgt
     4981 aaaaacgcct gggggcatac atgagcgaca cgatgcacgt cagcggcgcg gtacagacca
     5041 gggccccgca ccagctacgt ccggatcagg acgagccgac tgcccagcgg ttccgcaccg
     5101 acgcccccgt tccttgtgaa tacgtgcacc gtccggtcgt cgacgacatt ctggtcacgt
     5161 cctggagtcg cgtggacgac tgtcatttct cggtcaccgc ccactggcca cgggaccacg
     5221 ggtatttcgc gccgcgccgt ggccgggact cgctcatcct gaccggtgaa accattcgcc
     5281 aggccggact gctgctctcg cacaccgaat tcaacgtgcc ggtgggacac cacttcatcc
     5341 tcggtgacct cgtctacacc atgcggcccg agcacctccc cgccgacggt ggacccacca
     5401 cgctgacgat cgacgtcgcc tgcagcagcc tgcgcacccg acgcaccacg ctggccagcg
     5461 gacgcttcga catgaccatg cgcgccgcgg accaggtcgt cgtggtcggg cactcccacg
     5521 tcaccgtggc ctcccccgcc gtctaccgcc ggatccgcgg ggagcatctg acggcccagc
     5581 gcgacgtcgg acccctgccg tcgcccgtcg ccccccagct gacaggcagc gacaccgagc
     5641 acgacgtcct actctccccc accaaccggc ccgggcgttg gcacctgcgc atcgcccccg
     5701 gccacgccgc ggtcgtcaac ccggccaacg accacgtccc gggcatggtg ctggtcgatg
     5761 ccgcacagca ggccgcgcac gccctgaccg cgcccgagcg cttcgcgccg cacaccttcg
     5821 acatgcagtt cagccgctac gccgagcacg gaaccccctg tgtcatcgag gcccgccggg
     5881 tcgcgtccgc gcccggcaca gcgaccgtcc gggtcacggg agttcaggac ggtgagccgg
     5941 tgttcctgtg caccctgacc gcgcaggacg aacgcggctg aagaaccgtg ccaggccggc
     6001 gtgccggcct tctgcgccgc atggcgcggg gccggaccgg ccaggcatcc tcacagcagg
     6061 cggatgggct gacgcacccg cacggaacgt cgcaccgcaa cgcccggcaa cgcaccccgt
     6121 tgccccccaa tcagcacgag atcgacagtc cgcgtcggcg cacgccggcc gcggaacgag
     6181 aaaggccaac gacgacatgg gtgacctcaa cggaaagacc gcactggtga caggttcgag
     6241 caggggcatc ggccggggca tagcccggcg cctcgctcgg gaaggtgcac tcgtcgccgt
     6301 gcactacaac accgatgacg tagcggccaa ggagaccttg gacggcatca aggccgaggg
     6361 cgggcgggcc ttcgtcatag gcgcggagct cggggtgccc ggcgatgcgg cggccctgtt
     6421 cgccgccttc gacgccgaac tcgccgcgta tggcgccgaa ccgggtctcg acatactcgt
     6481 gaacaacgcg gcgacgcggt tctccgggca catcggcgag accacggagg acgagttcga
     6541 ccgcaccgtc gcgatcaacc tcaaggcgcc gttcttcatc gtccagcacg ccctgcaccg
     6601 catgcgggac ggcggacgca tcatcaacat ctcgtcggcg gtgaccagta cggcgtaccc
     6661 gtcgtcgatc gcctacagcg tctccaaggg cggcatcgac acgctcaccc gtaccctcgc
     6721 caaggatctc ggcgcgcggg gcatcacggt gaacgccctc gccccgggct gggtcgagac
     6781 ccacgtgacg gcggcgcgga cggcggtgcc cgagcaacgg gcggcactgg cggcctattc
     6841 cgtgttcaac cgcatcgggc agcccaccga catcgccgac gtcgtcgcct tcctcgcctc
     6901 cgacgactcc cgttggatca ccggccagcg catcgacgtg accggcgggt cgatgctgtg
     6961 acgttcgggc ccgggtgctc cgacccccgt ccggagcgcc cgggccgggc cgcgccgtct
     7021 caggcggcgt ccgctgcgac gccttccagg tccaacagca gccgcttgcg cgccaggccg
     7081 cccgcgtatc cggtgagcga gccgtcggcg ccgatgacgc ggtggcaggg gcggaccacc
     7141 aggagcgggt tggcgccgat ggccgtcccg atggcgcgga cggcgccgcg cggggcgccg
     7201 atggcggtgg cgatcgcgcc gtaggtggtg gtcgtgccgc gcgggatggc ctccagcgcc
     7261 gtccagacgc gctgttggaa ctcggtgccg cgggggacgc actcgacggt gaacggtgcc
     7321 gtcgcgccgt cgaagtaccc gcggagctgg cgggcgatct cggcgaacgc ggccgggtcg
     7381 tgggtccagc cctcctggac gaccgccccg cgcttctggt ccggcaccga gagcgaggcc
     7441 agtgcggtgc cgccgggcgc ggtggccgac cgctcgccca ccagcaggag ttcgcccagg
     7501 gggctgtcga gggtggcgta cagggtcatg cgtcgatccg ttcttcctgg ggcgagtgcc
     7561 gggccggggt gccggcgggg tcggccgcgt ggaggtagtg ggtggcgtag gagcgccagg
     7621 ggctccagag tgccggagtg gtgcccgggg gcggtgtgcc gaggaggacg tcgggatcgc
     7681 ccagggcccg catccgcaca taaccggcgg tccacgggcc gatgcccggc agtgcgcgca
     7741 gggcgcgttc ggcccggtcg cggtcggtgc cggcgtccag gacgacgtcc cgggcggcga
     7801 gcgccgcggc gagggcccgc agtgcggcgg cccgcgacgg ggacggggcg agcccggcga
     7861 gcggggcccg ggccagttcc gcggcccgcg ggaacaggtg ggtgaggccg ccgacggggc
     7921 gcggcagcgg ctccccgtag cccgcgacca ggcgttcccc caggtcccgg ccggcggccg
     7981 gtccggtccg gcgggcgagg acggcccgca ccgccagttc gaccgggtcc acggtgcccg
     8041 gggagcgcag cccggggcaa cgggcgacca gcggggccag cacgggatcc gcaccgagcc
     8101 gttcgccgac cgcgtacggg tcggcgtcga ggtcgaagag gcggcggacg cgctgggtgg
     8161 cggtggtgag gtcgcgcagt tcggtgaggt ggaggcggca gtccagccaa ccgtcgccga
     8221 tgacctcgtc gacctccatg atgccggtgc cgttcggcag tcgcagggtc cgccggtagg
     8281 tgcggcggcc gggcgccccg gtgacctcct cgatcccgcc gagcgcctgc gcttgcagct
     8341 ggtcgaagac ctgccgggcc gcgtacgggc cgcggaacgc cagccgcagc ggcagtacgc
     8401 cgggcgtgcc ggacgggggc gtcgggccgc tacgggcccc ggggcggacc gcccgcagcc
     8461 cgctcggggt gagggcgtag acctccttga tggtgtcgtt gaactgccgg acactggcga
     8521 agccggcggc gaaggcgatc tcggcgatcg gcagggcggt ggtctgcaac aggacccgtg
     8581 cggtgtgcgc gcgctgggcc cgggccagtg cggtgggccc ggcgccgagt tcggcggtca
     8641 gttgccgttg gagttgccgg gcgctgtagc cgagccggga ggccagcccg gcgacgccct
     8701 cccggtcgac cacgccgtcg ccgatcagcc gcaccgcccg tcccacgacg tcggcccggg
     8761 cgttccactc ggccgagccg gggaccgcgt cggggcggca ccgccggcag gcccggtagc
     8821 cggagccctg ggccgccgcg gcggtcggga agaaggtgac gttgcgccgc cgcggggtgg
     8881 tggcggggca gctcggccgg cagtagatcc cggtggtgga caccgcgaag aagaacgccc
     8941 cgtcgaacct ggcgtcccgg ctccgcaccg cctcgtacct gctgtcctca ctcatcacac
     9001 cccccactgt gcgccatccc gcgcccgcgc tctggcggaa atcggacatg gcgtccggtg
     9061 cggtgggtgc ctcggtggtc ggcggacggt gcccgctcca ccggccgggg cggggccgtc
     9121 acgccggcgg catcggccag tcgtccaggg agccgccgcg ccactcgatc aggcgggggt
     9181 cgtccagcga gacgtcgtgc gccggatcgg cgacgcctgc gcggcgcagg aactccgcga
     9241 cgtcgccgcg gccgtgggcc aggccgacga tctgcccgtg cacggtcacc cggcgtccac
     9301 cggagggggt cggtgggtgc acgatgacag gcgggtgctc ggacatgcct ccagcgtgcg
     9361 ccgggctccg cgcgctcgca cgtcggcggc gtgatctcac ccggatggtt gggatcccgg
     9421 ccgggcggca ccgccggcgc cggtgcgggc gtggtcccgg tccggcccgg gacggcgggc
     9481 gcggacccga accgcgagcg agagccgagg acgccagtga acgaccccat ggaatccgcc
     9541 gagttctccc gggatccgta tccgctgctc gccgcgctcc gggcgcgcgg cccggtgcag
     9601 cgggtgcgga ccgggaagga ccgcacgacg tgggtggtga ccggttgggc cgaggcgcgc
     9661 gcggcgctgg ccgaccgacg gctgtcgaag gacaccgccc gctacttcgc cgaccggccc
     9721 tccgggcgcg atctggcacc ggcggtcagc cagagcatgc tggccaccga tccgccggac
     9781 cacggccggc tgcggaagct ggccgtggcg gccttcaccc cggcggcggt gggccggctg
     9841 gagccgcgga tccgggagat cgcggagggg ctggccgcgg agctggggcg gtcggcgggg
     9901 gcggccggcg gtccggtcga tctggtcgag gggttcgcgg tgccgctgcc gatcgcggtg
     9961 atctgcgaac tgctggcggt gccggaggcg gaccgggccg cggtgcgccg gtggtccgac
    10021 gacctgttcg cggcggcgga cccggacacc accgaccggg cctcgcacgc cctggccggg
    10081 tacgtggcgg agctgatcgc ggcccggcgc gcggcgcccg gggacgatgt gctcagcgcg
    10141 ctgatcgccg cccgggacgc cggcgaccgg ttgtccgagc gtgaactcgt ctcgctggcc
    10201 gtgctgttgg tggtcgccgg gcacgagacc acgacgcacc tgatcggcaa cgggacgctg
    10261 gccctgctcc gtgacgacgc gctgcgcgcc cgcctgcggg acgatccggg gctgctgccg
    10321 gccgcggtgg aggagttcct acggtacgag gcgccggtga cgctggcgac cttccgctac
    10381 gccaccgagg cgttcgacct cggcggggtc cgcatcggag cgggggacgt ggtgctcgtc
    10441 tccccgggtg gcgccaatcg cgatccggcg cggttcgacg agccggacgt cgtgcggctg
    10501 gcccggtcgg gcgcgggcgg gcacctgtcg ttcgggcacg gtccgcacca ctgcctgggg
    10561 gcgccgctgg cccgcgccga ggcgcggatc gcgttcgagg tcctgctcgc gcggttcccc
    10621 ggactgcggc tggcagcggg cgggggccgg ggcgccgggg gttccgacgg cgcggacggg
    10681 gtcaggtggc ggcgcacgcg gttgatgcgc ggaccggccc aactgccggt gctcctgggg
    10741 ccgttgcggg aggcgccgga gggtcggggc tgaccgctcc cgcggaggcg ggcagggcgg
    10801 acgccccttg cgcgcacccc atccattctc gtgcactatg aatcgatttg gtcgaaaaaa
    10861 gcggaatgta tgatgctggc gacctccagg gttacccctg gttcgccgac ccgtccgacc
    10921 gagagggtgc ccgtgggcaa gccgaagccg atacgtccgg cgcccgcgct ggcgctggtc
    10981 g
//
