LOCUS       NZ_CP119144            10936 bp    DNA     linear   CON 22-NOV-2023
DEFINITION  Streptomyces noursei strain C27 chromosome, complete genome.
ACCESSION   NZ_CP119144
VERSION     NZ_CP119144.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces noursei
  ORGANISM  Streptomyces noursei
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9627936)
  AUTHORS   Go,B. and Lu,Y.
  TITLE     Control effect and mechanism of Streptomyces NK27 against gray mold
            of grape
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9627936)
  AUTHORS   Go,B. and Lu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-FEB-2023) State Key Laboratory for Biology of Plant
            Diseases and Insect Pests, Institute of Plant Protection, Chinese
            Academy of Agricultural Sciences, Yuanmingyuan, Beijing 100193,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-OCT-2021
            Assembly Method                   :: ABySS v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 98x
            Sequencing Technology             :: Complete Genomics
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 14:00:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,442
            CDSs (total)                      :: 8,348
            Genes (coding)                    :: 8,203
            CDSs (with protein)               :: 8,203
            Genes (RNA)                       :: 94
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 46 of 145
            Pseudo Genes (incomplete)         :: 121 of 145
            Pseudo Genes (internal stop)      :: 12 of 145
            Pseudo Genes (multiple problems)  :: 30 of 145
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:17:19
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 753660
            Orig. end                         :: 764596
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP119144.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10936
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="8"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10936
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10936
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5936](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5936
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(419..433)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.74"
                     /tool="antismash"
     gene            873..1763
                     /gene="metF"
                     /locus_tag="P0D74_RS02915"
     CDS             873..1763
                     /EC_number="1.5.1.20"
                     /GO_function="GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA]"
                     /GO_process="GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="metF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573702.1"
                     /locus_tag="P0D74_RS02915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="WP_275461190.1"
                     /transl_table=11
                     /translation="MSTLRADVTGPAPSFSFEFFPPKTEAGTRTLWRTIRRVEALAPTF
                     VSVTYGAGGSSRDHTVALTERIAADTTLRPVAHLTAVGHSVAELRHIIGRYSDAGVRDV
                     LALRGDPPGDPDGPWAPHPAGLTHAHELVSLVKDTGSFSVGVAAFPEGHPRSPDPDTDV
                     RHFIGKCRAGADYAITQMFFHVEDYLRLRDRVAAAGCDTPIIPEIMPATDVRQIRRFAQ
                     LSHAAFPEELAHRLEAARDSPADGHRIGVDHATRMALRLLDEGAPGLHYITLNRSSAAL
                     DIHGHVCRSRSLHAC"
     gene            1753..3204
                     /gene="ahcY"
                     /locus_tag="P0D74_RS02920"
     CDS             1753..3204
                     /EC_number="3.13.2.1"
                     /GO_function="GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ahcY"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018105956.1"
                     /locus_tag="P0D74_RS02920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="adenosylhomocysteinase"
                     /protein_id="WP_275461191.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 2.3e-09, bitscore:
                     35.8, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPADTTRFKVADLSLAAFGRKEITLAEHEMPGLMAIRKEYAEQQP
                     LKGARITGSLHMTVQTAVLIETLVALGAQVRWASCNIFSTQDHAAAAIAVGPHGTPEAP
                     QGVPVFAWKGETLTEYWWCTEQALTWPGTSTGGPNMILDDGGDATLLVHKGVEYEKAGK
                     APAVETAENEEHHAILELLNRTLADSPQKWTRLASEIRGVTEETTTGVHRLYEMQRDGV
                     LLFPAINVNDAVTKSKFDNKYGCRHSLIDGINRATDVLIGGKTAVVCGYGDVGKGCAES
                     LRGQGARVIITEIDPICALQAAMDGYQVATLDEVVETADIFVTTTGNKDIILASDMARM
                     KHQAIVGNIGHFDNEIDMAGLAKLPGIVKDEVKPQVHTWTHPDGKVLIVLSEGRLLNLG
                     NATGHPSFVMSNSFADQTLAQLELFTKPEQYPTDVYVLPKHLDEKVARLHLDALGVKLT
                     TLRKEQADYIGVKVEGPYKPDHYRY"
     gene            complement(3239..3994)
                     /locus_tag="P0D74_RS02925"
     CDS             complement(3239..3994)
                     /EC_number="1.1.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (4-171). E-value: 5.8e-15. Score:
                     47.6. Matches aSDomain:
                     nrpspksdomains_P0D74_RS02925_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010644115.1"
                     /locus_tag="P0D74_RS02925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_275467463.1"
                     /sec_met_domain="adh_short (E-value: 1.5e-35, bitscore:
                     121.7, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 2.7e-31, bitscore:
                     108.3, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNRKTIMITGASSGIGAAAAHCFAEEGASVVLMARREDRLRALAG
                     EIEETGGQVAVVTGDVTNQADVDRAVDVAVAEFGTLDAAFNNAGQGTLGAPLHETEQAV
                     FDRIMDVNVRGVWNCLRAQIRTMLTQGTGGAIVNTSSTAGLFATGAVAPYVAAKHAVLG
                     LTKTAAAEYGDHGIRVNALVVGATRTDMVASVLQEVDGLREADLVARAIQRRLADPVEV
                     ARAAAWLCSDQASFITGGVVPVDGGWSAV"
     aSDomain        complement(3482..3982)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_P0D74_RS02925_PKS_KR.1"
                     /evalue="5.80E-15"
                     /label="P0D74_RS02925_PKS_KR.1"
                     /locus_tag="P0D74_RS02925"
                     /protein_end="171"
                     /protein_start="4"
                     /score="47.6"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TIMITGASSGIGAAAAHCFAEEGASVVLMARREDRLRALAGEIEE
                     TGGQVAVVTGDVTNQADVDRAVDVAVAEFGTLDAAFNNAGQGTLGAPLHETEQAVFDRI
                     MDVNVRGVWNCLRAQIRTMLTQGTGGAIVNTSSTAGLFATGAVAPYVAAKHAVLGLTKT
                     AAAE"
     CDS_motif       complement(3887..3982)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_P0D74_RS02925_0001"
                     /evalue="2.00E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="P0D74_RS02925"
                     /protein_end="36"
                     /protein_start="4"
                     /score="10.9"
                     /tool="antismash"
                     /translation="TIMITGASSGIGAAAAHCFAEEGASVVLMARR"
     gene            complement(4247..4870)
                     /locus_tag="P0D74_RS02930"
     CDS             complement(4247..4870)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573072.1"
                     /locus_tag="P0D74_RS02930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_275461192.1"
                     /transl_table=11
                     /translation="MSRQRQDRALQTRAAIVRAAAEVFAEQGYAGASVTKITERAGLTM
                     GAMYFHFKNKAALAREIVVGQPDRVLPRHESVGLQRAVDITLAWAHQIREDPVLLAGAR
                     LVMEQEEFIGQEQNSHQQWAQIFEQELAEAQKRGELTGTCDESAVARLIVNACTGAQMH
                     AHMLTGYRDLPIRVVEMWSCLLPSLATPEAAASIDLNTERGRSM"
     gene            5001..5936
                     /locus_tag="P0D74_RS02935"
     CDS             5001..5936
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="P0D74_RS02935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_275461193.1"
                     /sec_met_domain="AfsA (E-value: 4.2e-19, bitscore: 67.3,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPAPLAVPVASLPEAASDLGPLVRKRVADEVLITDWRTVSGTTHH
                     VTAHWPQHHSFYTPRSGRYSSLLLVETVRQALALLSNAAYEVPLEYRLGWEYCSCTLTP
                     AMLDTRPDGRGAEVELTITHALPAARRKGGSVRLEAQITAVRDGQLLGAAHLRYTCHSP
                     AIYERLRGQYADARAATSHAPLPGTPVPPAHVGRTSTKDVVLCPTAAPRRWQLRVDTDH
                     PVLFDHAHDHLPGMVLLEAAGQAAHCLAGPDPTTPVTLRTHFTRYTELDAPCWIEATPL
                     EWPQPDLARTTITGTQNGQVTFTTQVITRR"
     misc_feature    5202..5204
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(6009..6761)
                     /locus_tag="P0D74_RS02940"
     CDS             complement(6009..6761)
                     /NRPS_PKS="Domain: PKS_KR (8-159). E-value: 2.1e-14. Score:
                     45.8. Matches aSDomain:
                     nrpspksdomains_P0D74_RS02940_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019433623.1"
                     /locus_tag="P0D74_RS02940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_275461194.1"
                     /sec_met_domain="adh_short (E-value: 3.7e-34, bitscore:
                     117.2, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVRETGRVAVVTGGTKGIGLAFTRKLVKLGYRVVALYRHDPEAA
                     RAAAAELGDACTVLQADVSDSGEVNRLADGVLAHHGVPHVLVNNAGRNVDRPFAEMSDD
                     AWHTVLNTNLSGPFYVARAFAPAMREAGSGVIVNIAATTGIRPRFNGVNYCASKAGLLH
                     LTKCLALELAPHVRVNALIPGMIDTEDMQMRVRTAAPEHRASMLTSIPQERIGTVEEMA
                     AALEFLVGDGAEYVTGQKLIVDGGQFMW"
     aSDomain        complement(6285..6737)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_P0D74_RS02940_PKS_KR.1"
                     /evalue="2.10E-14"
                     /label="P0D74_RS02940_PKS_KR.1"
                     /locus_tag="P0D74_RS02940"
                     /protein_end="159"
                     /protein_start="8"
                     /score="45.8"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VAVVTGGTKGIGLAFTRKLVKLGYRVVALYRHDPEAARAAAAELG
                     DACTVLQADVSDSGEVNRLADGVLAHHGVPHVLVNNAGRNVDRPFAEMSDDAWHTVLNT
                     NLSGPFYVARAFAPAMREAGSGVIVNIAATTGIRPRFNGVNYCASKA"
     CDS_motif       complement(6660..6731)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_P0D74_RS02940_0001"
                     /evalue="8.50E-06"
                     /label="PKSI-KR_m1"
                     /locus_tag="P0D74_RS02940"
                     /protein_end="34"
                     /protein_start="10"
                     /score="18.4"
                     /tool="antismash"
                     /translation="VVTGGTKGIGLAFTRKLVKLGYRV"
     misc_feature    6751..6764
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.27"
                     /tool="antismash"
     misc_feature    7090..7104
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.38"
                     /tool="antismash"
     gene            7184..7999
                     /locus_tag="P0D74_RS02945"
     CDS             7184..7999
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573069.1"
                     /locus_tag="P0D74_RS02945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="WP_275461195.1"
                     /transl_table=11
                     /translation="MDKPIIAYLSDIGNHDEAHALGKGLIKTIAPGAEIVDITHQVTPF
                     DVREGGLYLQDVPASFPANTVIAAYVYPETGTSTRTVVVRNEKGQLLVAPNNGLLTWAL
                     KAVPAVEAWEVTSPDVMNQPVTPTWYGKDVVVACGAHLAAGVAPSAVGPKIDVAKLVTL
                     PTTPAVQLGDGSVRGEVVRIDKAFGNVWTNISLDALSGGGALDGKTLQVTAEGLSVEIP
                     YYATFGEVPIGEPLVYNNSRGKVALGLNQGSFLKRYGVAAGDTVTIGLV"
     gene            8094..8735
                     /locus_tag="P0D74_RS02950"
     CDS             8094..8735
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573068.1"
                     /locus_tag="P0D74_RS02950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_275461196.1"
                     /transl_table=11
                     /translation="MDSQEIVDRSHSLDGRPELLSAFYGDWAETYENDASNMGYEGPLR
                     MADFYLSLAPRMLPAPEATTALDAGCGTGLVGPVFKKLGIGSLDGLDLNHEMVTQAAGT
                     GAYRLLTSGTDLNLGLPEFAPDTYDLTLSCGVFTTGHVAPNALDALLRLTRPNGLVLLT
                     TSANYLSVTDFHGFLESRAAQRRLTVIECLADHHYLTDERAHYWAISPTA"
     misc_feature    8974..8989
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.35"
                     /tool="antismash"
     gene            9184..9591
                     /locus_tag="P0D74_RS02955"
     CDS             9184..9591
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573067.1"
                     /locus_tag="P0D74_RS02955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_275461197.1"
                     /transl_table=11
                     /translation="MAVKETIQVDESQKNEPGVQEVITPVPVGSEVIKKATYWRSILQD
                     DLDPQATDGVTTVKLAVPALVEEEYETGETNEDGTAKLGVRQVRDTQWYEIDLSEANVA
                     ALQEAVKPFTDVARTIEAPTVKPARKKRTTK"
     gene            9806..10189
                     /locus_tag="P0D74_RS02960"
     CDS             9806..10189
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007379595.1"
                     /locus_tag="P0D74_RS02960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AQJ64_40280 family protein"
                     /protein_id="WP_275461198.1"
                     /transl_table=11
                     /translation="MTNLHTVVTWIDARERLPRSGAPVAAAITGRYPADDAGGSDAGSG
                     EEFWLVRPMYFTTLHRGEDGVEHRDCFVDSDGIVRLPHGLASAETVTHWAELPVLPGQQ
                     THIALGGDVQPALRNAWDAHPTT"
     gene            complement(<10232..>10342)
                     /locus_tag="P0D74_RS02965"
                     /pseudo=""
     CDS             complement(<10232..10342)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573065.1"
                     /locus_tag="P0D74_RS02965"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="zinc-binding dehydrogenase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MVLTLHVADVLPAEEAAEAHRRLEAGGLRGRLVLDFS"
ORIGIN
        1 cgggacgtgc aggtctcgga gccggagccg gagcagggcg cggtccgttc cgacgtggcc
       61 gtcgacaatg cggtgcccac gccgccgttc tggggcaccc gggtcatcaa gggcatccag
      121 ctcaaggagt acgcctcctg gctggacgag ggcgccctgt tcaagggcca gtgggggctc
      181 aagcaggccc gcaccggcga gggccccagc tacgaggaac tggtggagag cgagggccgg
      241 ccgcggctgc gcggctggct cgacttcctg cacaccagga acctgctcga agccgccgcc
      301 gtccacggct acttcccctg tacctccaag ggcgacgact tgatcatcct ggacgaggac
      361 ggcaccgagc ggacgcggtt caccttcccg cggcagcggc gcggccggcg cctgtgcctg
      421 gccgacttct tccgtcccga ggactccggc gagaccgacg tggtcggcct ccaggtcgtc
      481 accgtcggtt cgcggatcgg cgtggccacc gccgagctgt tcgaggccaa ctcctaccgc
      541 gactacctcg aactgcacgg cctgtccgtc cagttggcgg aggcgctggc cgagtactgg
      601 cacgcccggg tccgcggcga actcggcttc ggcggggagg acccgaccga cgtcgaggac
      661 atgttcgcgc tcaagtaccg cggcgcccga ttctccctcg gctacggcgc ctgccccgac
      721 ctggaggacc gcgccaagat cgccgacctc ctccagcccg aacggatcgg cgtccagctc
      781 tccgaggagt tccagctcca ccccgagcag tccaccgacg ccctcgtcat ccaccacccg
      841 gaagccaagt acttcaacgc ggggagtcgt tcatgagcac gctccgtgca gacgtcaccg
      901 gtccggcacc gtcgttctcc ttcgagttct tcccgcccaa gaccgaggcc gggacgcgca
      961 ccctgtggcg gacgatccgg cgcgtcgagg cgctggcacc gacgttcgtg tcagtcacct
     1021 acggcgcggg cggttcctcc cgagaccaca ccgtcgcgct caccgagcgg atcgccgccg
     1081 acaccaccct ccgcccggtc gcgcacctca cggccgtcgg ccactccgtc gccgaactgc
     1141 gccacatcat cgggcggtac tccgacgcgg gggtgcgcga cgtcctcgcc ctgcggggcg
     1201 atccaccggg ggatcccgac gggccctggg cgccgcatcc cgccggtctc acccacgcac
     1261 acgaactggt ctcgctcgtg aaggacaccg gctcgttctc ggtcggcgtc gccgccttcc
     1321 ccgaaggcca cccccgctcg ccggacccgg acaccgacgt ccggcacttc atcggcaagt
     1381 gccgggccgg tgccgactac gccatcacgc agatgttctt ccacgtcgag gactacctgc
     1441 ggctacgcga ccgggtggcg gcagccggct gcgacacccc gatcattccc gagatcatgc
     1501 ccgccacgga cgtgcggcag atccgccgct tcgcccaact cagccacgcc gccttccccg
     1561 aggaactcgc gcaccgcctg gaggcggcac gcgacagccc cgcggacggt caccgcatcg
     1621 gcgtggacca tgccacgcgg atggcgttgc ggttgctcga cgagggcgca ccagggctgc
     1681 actacatcac cctcaaccgg tcgagcgccg ccctcgacat ccatggtcac gtctgccgtt
     1741 caaggagtct ccatgcctgc tgacaccacc cgtttcaagg tcgctgacct ttccctcgcc
     1801 gccttcggcc gcaaggagat caccctcgcc gagcacgaga tgcccggtct gatggcgatc
     1861 cgcaaggagt acgccgagca gcagcccctg aagggcgcgc ggatcactgg ttcgctgcac
     1921 atgaccgtgc agaccgccgt gctgatcgag acgctggtcg ccctgggtgc gcaggtgcgc
     1981 tgggcgtcct gcaacatttt ctcgactcag gaccatgcgg ccgcggcgat cgcggtgggt
     2041 ccgcacggta cgcccgaggc gccgcagggt gtgccggtgt tcgcctggaa gggcgagacg
     2101 cttacggagt actggtggtg cacggagcag gcgctgacct ggccgggcac gtccaccggt
     2161 ggtccgaaca tgattctcga cgacggtggt gacgccaccc tgctggtgca caagggcgtc
     2221 gagtacgaga aggcgggtaa ggctccggcg gtggagaccg cggagaacga ggagcaccac
     2281 gcgatcctgg agctcctcaa ccgcaccctc gcggacagtc cgcagaagtg gacccggctg
     2341 gcgtcggaga tccgtggggt gacggaggag accacgaccg gtgtccaccg tctgtacgag
     2401 atgcagcgtg acggcgtgct gctcttcccg gcgatcaacg tcaatgacgc ggtgaccaag
     2461 tcgaagttcg acaacaagta tggctgccgg cactccctca tcgacggcat caaccgcgcc
     2521 actgatgtcc tgatcggtgg taagacggcc gtggtctgcg gttacggtga cgtcggcaag
     2581 ggttgcgcgg agtcgctgcg cggccagggc gcccgcgtga tcatcaccga gatcgacccg
     2641 atctgtgcgc tgcaggcggc gatggacggt taccaggtcg cgacgctgga cgaggtcgtg
     2701 gagacggctg acatcttcgt gaccacgact ggtaacaagg acatcatcct ggcgtcggac
     2761 atggcgcgga tgaagcacca ggcgatcgtc ggcaacatcg gtcacttcga caacgagatc
     2821 gacatggcgg gtctggcgaa gctgccgggt atcgtcaagg acgaggtcaa gccgcaggtc
     2881 cacacctgga cccacccgga cggcaaggtg ttgattgtgc tgtccgaggg tcgtctgctg
     2941 aacctgggca atgccaccgg tcacccgtcg ttcgtgatgt cgaactcgtt cgcggaccag
     3001 acgctggcgc agttggagct gttcaccaag cccgagcagt acccgaccga cgtctacgtg
     3061 ctgcccaagc acctggacga gaaggtcgcc cggctccacc tggacgcgct gggcgtcaag
     3121 ctcaccaccc tgcgcaagga gcaggccgac tacatcggcg tcaaggtcga gggcccgtac
     3181 aagcccgacc actaccgcta ctgaccgcca ccgccgaccc acccacgggc cgaggcgctc
     3241 agaccgccga ccagcccccg tccacgggca ccactcctcc cgtgatgaag gacgcctggt
     3301 cgctgcagag ccaggcagcg gcgcgagcga cctcgacggg gtcggccagc cggcgctgga
     3361 tggcccgggc gacgaggtcc gcctcccgga gcccgtccac ctcctgtagg acggaggcga
     3421 ccatgtcggt gcgggtagcc cccacgacga gggcgttgac acggatgccg tggtcgccgt
     3481 actcggcggc cgcggtcttc gtcagcccca ggacggcgtg cttggcggcc acgtacggag
     3541 cgaccgcacc ggtcgcgaac aggcccgccg tgctggaggt gttgacgatc gcgccgccgg
     3601 ttccctgggt gagcatcgtg cggatctggg cgcgcagaca gttccagacg cccctgacgt
     3661 tgacgtccat gatccggtcg aagaccgctt gctccgtctc gtgcagggga gctccgagcg
     3721 tgccctgtcc ggcgttgttg aaggcggcgt cgagggtgcc gaactcggcc accgccacgt
     3781 cgaccgcacg gtcgacgtca gcctggttgg tgacgtcgcc ggtcaccacc gcgacctggc
     3841 cgccggtctc ctcgatctcc ccggccaggg cccggagccg gtcctcgcgg cgggccatca
     3901 ggacgacgga tgcgccttcc tccgcgaagc agtgcgcggc ggccgccccg atcccgctcg
     3961 acgcaccggt tatcatgatc gtttttcggt tcagcgtgcc ccgtccgcgg tcgtcttcgc
     4021 ggtgtctcgg caccgccggc ccacgggacg cacgccgccc ctgctctgcg ctctgctgcc
     4081 tcggtgtggt ccctgtcgcc tgtgctggaa tcgtcggcgt catcccatgc ccccgtatgt
     4141 ggacagccac tagttgggaa ttatacgaat cccagttcgc caccgtagtg cactcgccgc
     4201 gcctcgctcg ggtcaacgtc tctttgcgat tgaaactgat gactcgtcac atcgacctcc
     4261 cacgctcggt gttcaggtct atcgaggccg ccgcctccgg tgtggctagc gacgggagca
     4321 ggcagctcca catctccacc acacggatgg gcagatcacg gtaaccagtc agcatgtggg
     4381 catgcatctg cgccccggtg cacgcattga cgatcagacg cgccaccgcc gactcgtcgc
     4441 acgttcccgt gagttcaccg cgcttttggg cttcggccaa ctcctgctcg aatatctgcg
     4501 cccactgctg gtgggagttc tgctcctggc ctatgaactc ctcctgctcc atgaccagcc
     4561 gcgcgccggc gagcagcacg gggtcttctc ggatctggtg cgcccatgcc agagtgatgt
     4621 cgacggcgcg ctggagcccg acggactcgt gccgcggcag cacccgatcc ggctgcccca
     4681 cgactatctc gcgcgccaac gccgccttgt tcttgaagtg gaagtacatg gcgcccatcg
     4741 tcagacccgc acgttcagtg atcttggtga cgctggcgcc cgcgtagccc tgctccgcga
     4801 agacctcggc cgccgcccgc actatggcgg cccgggtctg gagggcacgg tcctgtcgct
     4861 gtcttgacat gcatctttcc ggtctgctct cgacagaata aataacgttc tctatgttac
     4921 ggcacacggc tggttcaact ccatgccctt ccacaccagt tgctgcacac gacccttcac
     4981 cacccttgag gagaagtcgt atgcctgccc ccctcgccgt ccccgtcgcc tcccttcccg
     5041 aagcggcctc ggacctgggt cccctggtcc gcaagcgcgt cgccgacgaa gtactgatca
     5101 ctgactggcg cacggtctcc ggcaccaccc accacgtcac cgcacactgg ccgcaacacc
     5161 acagcttcta cacgccgcgt tcgggacgct acagctccct gttactggtc gagaccgtgc
     5221 gccaggccct cgcactgctc tccaacgcgg cctacgaggt gccgctggag taccggttgg
     5281 gctgggaata ctgctcgtgc accctcactc cggccatgct ggacacccga ccggacggtc
     5341 gcggggcgga ggtcgaactg accatcacgc acgccctgcc cgccgcgcgc cgcaagggcg
     5401 gttccgtccg cctggaggca cagatcaccg cggtccgcga cggccagctg ttgggcgcag
     5461 cgcacctgcg ctacacctgc cactcacccg ccatctacga gcgtctgcgc ggccagtacg
     5521 ccgacgcccg ggcagcgacc tcgcacgcac cgctccccgg cactccggtg ccgcccgcgc
     5581 atgtgggccg cacgagcacc aaggacgtcg tgctctgccc gacggcggcc ccccggcgct
     5641 ggcaactacg ggtcgacacc gaccacccgg tgctcttcga ccacgcccac gaccacctgc
     5701 ccggcatggt gttgctggag gccgccggcc aggccgcgca ctgcctcgcc ggacccgacc
     5761 ccaccacacc ggtgacgctc cgcacgcact tcacgcgcta caccgagttg gacgcgccct
     5821 gctggatcga ggcgacgccg ctggagtggc cgcagccgga cctcgcccgc accacgatca
     5881 ccggcaccca gaacgggcag gtcaccttca ccacccaggt gataacccga cgctgagggg
     5941 gttcccggct ccaggacggc gtgagggggc cgccgtcggc agccccctca ggtcctccgg
     6001 acgctccgtc accacatgaa ctggccgccg tcgacgatga gcttctggcc cgtgacatat
     6061 tcggccccgt cgccgacgag gaactccagc gccgccgcca tttcctcgac cgttccgatg
     6121 cgttcctgcg gaatgctcgt cagcatgctc gcgcggtgct cgggcgccgc ggtccgcacc
     6181 cgcatctgca tgtcctcggt gtcgatcatg ccggggatca gcgcgttcac gcggacgtgc
     6241 ggagcgagct ccaacgccag gcacttcgtc aggtggagca gcccggcctt gctcgcacag
     6301 tagttgaccc cgttgaaccg gggcctgatg ccggtcgtcg cagcgatgtt gacgatgacg
     6361 ccgctgccgg cctcgcgcat cgcgggggcg aacgcccgcg ccacgtagaa ggggcccgac
     6421 aggttggtgt tgaggaccgt gtgccacgcg tcgtccgaca tctcggcgaa cggccggtcg
     6481 acgttccggc ccgcgttgtt gaccaacacg tgcggcacac cgtggtgcgc cagcacgccg
     6541 tcggccagtc ggttgacctc gccgctgtcg gagacgtcgg cctggagcac cgtgcaggcg
     6601 tcgccgagct cggcggcggc cgcccgcgct gcctcgggat cgtgccggta cagcgcgacg
     6661 acccggtagc cgagtttcac gagcttccgg gtgaatgcca gaccaattcc cttggtacca
     6721 cccgttacga cggccactcg tccggtttca cgcacactca tgcaatccag tcctctctac
     6781 ggcggaattc atccgagaac tcccggcaca gtgcgcggcg agccctccgg gaactccggg
     6841 actctcttcg gtgcgcggag ccgtcacacg acgttcccaa ggcggcgccc aggcgccgct
     6901 gccggcgcca cggcattccg ccaccgacgc atctctccca cgccaacaga gccttcgcgg
     6961 ccctgcccgt gagcgatccg gcccgccacg cgtcccatca tcgacggccg gggtcgcaaa
     7021 cggcaatgtc cctaaggtgg cattggattt tgtcaccatc gccgaatggt gccgggccgg
     7081 cgagacgaat catcatgatg aaaacccggc cggaatcccg gacccacacc ggatccgttc
     7141 cggccggaat cggaacattc ctggaccacg gaaggtcgaa tcggtggaca agcccatcat
     7201 cgcttacctg tcggacatcg gtaaccacga cgaggcgcac gcactcggca agggcctcat
     7261 caagaccatt gccccgggcg cagagatcgt cgacatcacc catcaggtca ccccgttcga
     7321 cgtgcgggag gggggcctgt acctgcagga cgtgccggcg tcgttccccg ccaacaccgt
     7381 catcgccgcc tacgtctacc ccgagaccgg cacgtcgacc cgcaccgtcg tggtccgcaa
     7441 cgagaagggc cagctgctcg tcgcccccaa caacgggctg ctgacctggg ccctgaaggc
     7501 cgtcccggcc gtcgaggcgt gggaggtgac ctcgccggac gtgatgaacc agcccgtcac
     7561 cccgacctgg tacggcaagg acgtcgtggt ggcctgcggt gcccacctgg ccgccggggt
     7621 cgccccgtcc gccgtcggac ccaagatcga cgtggccaag ctggtcaccc tgccgacgac
     7681 cccggccgtg cagctcggcg acggttcggt gcgcggcgaa gtcgtccgga tagacaaggc
     7741 gttcggcaac gtctggacga acatctccct ggacgcgctg tccggcggcg gggcactgga
     7801 cggcaagacc ctccaggtga cggccgaggg actgtccgtg gagatcccgt actacgccac
     7861 cttcggcgag gtgccgatcg gcgagccgct ggtctacaac aacagccgag gcaaggtcgc
     7921 cctcggcctc aaccagggca gcttcctcaa gcggtacggc gtggccgcgg gcgacaccgt
     7981 gaccatcggt ctcgtctgag tcgacccagt gcacgaacca cccatcccac ctgatgcacg
     8041 aaggcgcctg gcccggtatg gggccaggcg ccttggtgcg gaggagacag cacatggaca
     8101 gccaggagat cgtggaccgc tcgcacagcc tcgacgggcg ccccgaactg ctcagcgcct
     8161 tctacggcga ctgggccgaa acctatgaga acgacgcgag caacatgggt tacgaggggc
     8221 cgctgcgcat ggccgacttc tacctctcgc tcgcgccccg catgctgccg gcccccgagg
     8281 cgaccaccgc gctcgacgcg gggtgcggaa cgggcctggt cggcccggtc ttcaagaagc
     8341 tcggcatcgg cagcctcgac ggactggacc tcaaccacga gatggtcacg caggccgcgg
     8401 ggacgggcgc ctaccgcctc ctcaccagcg gcaccgacct caacctgggc ctgccggaat
     8461 tcgcacccga cacctacgac ctcaccctca gctgcggcgt gttcaccacc gggcacgtgg
     8521 cgccgaacgc attggacgcg ctcctgcgcc tcacccgccc gaacggcctg gtgctcctca
     8581 ccacgagcgc caactacctc tccgtcacgg atttccacgg cttcctggag tcccgggcgg
     8641 cgcagcggcg gctgaccgtg atcgagtgcc tggccgacca ccactacctc accgacgagc
     8701 gcgcacacta ctgggcgatc tcccccaccg cctgagcggt cgtccgcttc cccaccgggc
     8761 gagaggagcc ccgccccgcc gggtgccgat cggctcggcc ggggaacgcg cccagtcctg
     8821 cgccccctgc ccgcggcccg gcggccgaat gccggccgct tcgccgcttt cccgacctct
     8881 ttgcgcacat cgcgcgtgcg gttccccggg ccgcgggaga agaacgcgaa gtcgcccggg
     8941 ataaagagga ccctctctct tcaccgagga cctttaaggc tttctggacc tctacgcgtc
     9001 acacccattc cgtgaagacc accaacggcg ccgccgcgaa aagaagtgag cactccggga
     9061 tcagccggag agttgaccag ggacatccgc aatcaagggc ctctcgcacg ggccgggctc
     9121 gattggcttc aactccccca tttaccttag aatttgggtg caccaatcga aaggggcgcc
     9181 gttgtggccg tgaaggaaac catccaggtc gacgaatctc agaagaacga gccgggagtg
     9241 caagaggtca tcactcccgt gccggtcggc agtgaggtca tcaagaaggc aacgtactgg
     9301 cggtcgatcc tccaggacga cctcgacccc caggcgacgg acggcgtcac gaccgtgaag
     9361 ctcgccgtgc cggccttggt cgaggaggag tacgaaaccg gagagaccaa cgaagacggc
     9421 acggcgaagc tcggtgtccg tcaggtccgt gacacgcagt ggtacgagat cgacctcagc
     9481 gaagccaacg tggcagcgct ccaggaggcg gtcaagccct tcaccgacgt ggcccgcacg
     9541 atcgaggccc ccacggtgaa gcctgcacgc aagaagcgca caaccaagtg attgtcggcg
     9601 ccccctcgca gcgagggggc gttcttgttg tgcggggact tgtccccttg gttccccttg
     9661 gttcccatgc tctgcacgtt ccgccgcggg caaccatcgc gggcgccgcg cggtccttcc
     9721 ggatgtcggg gtcggtccgg cgcccccgcc gacgcgccgc ccccgaacaa gaggcgcgtt
     9781 cccgagtgtt ggggtgagga gcgggatgac gaatctccat accgtcgtga cgtggatcga
     9841 cgcacgcgag aggctgcccc ggagcggtgc gccggtggca gcggcgatca cgggccggta
     9901 cccggcggac gacgccggcg ggtccgacgc cggatcgggg gaggaattct ggctggtgag
     9961 gccgatgtac ttcaccacgc tgcaccgggg cgaggacggg gtcgagcaca gggactgctt
    10021 cgtcgactcc gacggaatcg tccgcctgcc ccacggcctc gccagtgccg agacggtgac
    10081 gcactgggcc gaactgcccg tgctgcccgg ccagcagacg cacatcgcac tgggagggga
    10141 cgtgcagcca gccctgcgca acgcctggga cgcccacccc accacctgac cgacgccccc
    10201 agacccgttg acgtcgcgtg cgggaccgct gggagaagtc cagcaccagg cggccgcgca
    10261 ggccgcctgc ttccaggcgg cggtgggcct cggccgcctc ctcggcgggc agcacgtcgg
    10321 cgacgtgcag cgtcaggacg cccccggcgt ccgcgatgac ggtcaggccg tcgcctgggg
    10381 ttcgggcaac tccatgacgc gcagggcttg cgggccgccg aacacgtcaa cacctatggc
    10441 tttcatgctg gtttcaaccg gcctcgccga cgcggcattc ccttgttccc accgtgacgg
    10501 caatccgccg gggcgcgctc ccggacgtgg cccggggtcg gccggcggga gcgtggggcg
    10561 tcggcgcggg gtcaggtcac cggccgggtg tcgcggaaga gtttgccggt ggggccgttc
    10621 tcgcccaggg tcgccgccca gacgacgccg cgtgcgctgt cggcggcggg acgcgcgtgc
    10681 ccctcgtcgc cgcgttcggg gtgggtggcg gtttcgcccg ggtcgacggc gttgacgagg
    10741 atcggcgtgt ccttcagggc tcgggccagg acggtggtga ggacgtcgag ggtgtgcttg
    10801 gcgaacgagt gggccggggc gcggaggctg gccccgaggt cgagcccagt ggcgatctgt
    10861 tgggcggaga tgctggtgac gttgacgatg cgggcggcgg gggcggccgt cagcagcggc
    10921 agcagcgcct ggacca
//
