LOCUS NZ_JARDXE010000018 10888 bp DNA linear CON 01-APR-2024 DEFINITION Rhodococcus qingshengii strain HQ HQ1_scaffold18, whole genome shotgun sequence. ACCESSION NZ_JARDXE010000018 VERSION NZ_JARDXE010000018.1 KEYWORDS WGS; RefSeq. SOURCE Rhodococcus qingshengii ORGANISM Rhodococcus qingshengii Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Nocardiaceae; Rhodococcus; Rhodococcus erythropolis group. REFERENCE 1 (bases 1 to 163268) AUTHORS Hu,J. and Xu,J. TITLE A novel hydrolase synthesized by Rhodococcus erythropolis HQ is responsible for the detoxification of Zearalenone JOURNAL Unpublished REFERENCE 2 (bases 1 to 163268) AUTHORS Hu,J. and Xu,J. TITLE Direct Submission JOURNAL Submitted (23-FEB-2023) Grain and Oil Risk Assessment Laboratory, Jiangsu Academy of Agricultural Sciences, No. 50, Zhongling Street, nanjing 210014, China COMMENT ##Genome-Assembly-Data-START## Assembly Date :: MAY-2022 Assembly Method :: ABySS v. 2.1.5 Genome Representation :: Full Expected Final Version :: Yes Reference-guided Assembly :: GCA_002079265.1 Genome Coverage :: 470.0x Sequencing Technology :: Illumina NovaSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI RefSeq Annotation Name :: GCF_029076895.1-RS_2024_04_01 Annotation Date :: 04/01/2024 06:35:53 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 6.7 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total) :: 6,927 CDSs (total) :: 6,855 Genes (coding) :: 6,768 CDSs (with protein) :: 6,768 Genes (RNA) :: 72 rRNAs :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs :: 1, 1, 1 (5S, 16S, 23S) tRNAs :: 66 ncRNAs :: 3 Pseudo Genes (total) :: 87 CDSs (without protein) :: 87 Pseudo Genes (ambiguous residues) :: 0 of 87 Pseudo Genes (frameshifted) :: 33 of 87 Pseudo Genes (incomplete) :: 63 of 87 Pseudo Genes (internal stop) :: 12 of 87 Pseudo Genes (multiple problems) :: 18 of 87 ##Genome-Annotation-Data-END## ##antiSMASH-Data-START## Version :: 8.dev-cf2fc5ee(changed) Run date :: 2025-09-12 19:34:22 NOTE :: This is a single region extracted from a larger record! Orig. start :: 110872 Orig. end :: 121760 ##antiSMASH-Data-END## REFSEQ INFORMATION: The reference sequence is identical to JARDXE010000018.1. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ FEATURES Location/Qualifiers region 1..10888 /candidate_cluster_numbers="1" /contig_edge="False" /product="butyrolactone" /region_number="1" /rules="AfsA" /tool="antismash" cand_cluster 1..10888 /candidate_cluster_number="1" /contig_edge="False" /detection_rules="AfsA" /kind="single" /product="butyrolactone" /protoclusters="1" /tool="antismash" protocluster 1..10888 /aStool="rule-based-clusters" /category="other" /contig_edge="False" /core_location="[5000:5888](+)" /cutoff="20000" /detection_rule="AfsA" /neighbourhood="5000" /product="butyrolactone" /protocluster_number="1" /tool="antismash" proto_core 5001..5888 /aStool="rule-based-clusters" /tool="antismash" /cutoff="20000" /detection_rule="AfsA" /neighbourhood="5000" /product="butyrolactone" /protocluster_number="1" gene 409..822 /locus_tag="PXH69_RS25585" /old_locus_tag="PXH69_25590" CDS 409..822 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003939933.1" /locus_tag="PXH69_RS25585" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_25590" /product="cupin domain-containing protein" /protein_id="WP_058226656.1" /transl_table=11 /translation="MEILKPQPTSKAPSDWFTGDVWWDVIYAGQEPSKMRANMVRFAPC ARTDWHSHALGQTLHIVSGTALVQARGGEIVEVHPGETIYTPAGEEHWHGAAPDRFMTH LALWEGPGGGSTETTWGDKVTDEEYGGPRARRR" gene complement(836..1526) /gene="lexA" /locus_tag="PXH69_RS25590" /old_locus_tag="PXH69_25595" /pseudo="" CDS complement(836..1526) /GO_function="GO:0004252 - serine-type endopeptidase activity [Evidence IEA]" /GO_process="GO:0006282 - regulation of DNA repair [Evidence IEA]" /GO_process="GO:0006355 - regulation of DNA-templated transcription [Evidence IEA]" /codon_start=1 /gene="lexA" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003940361.1" /locus_tag="PXH69_RS25590" /note="frameshifted; Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_25595" /product="transcriptional repressor LexA" /pseudo="" /transl_table=11 /translation="MSGPLTTILIVTDYDDLDMFGGLDASSLPLRQQQILMTIRDLVAA NGCTPSTRQIGDAVGLRSTSTVSKHLKSLEEKGFLRRGAAMARQLDVRPFLVGAKEQSS SNTVTVPVVGDIAAGAPILAEEHADEVLALPRELVGSGTVFGLRVRGESMVDAAICDGR RRRGAPPGRSSFR" gene 1604..2074 /locus_tag="PXH69_RS25595" /old_locus_tag="PXH69_25600" CDS 1604..2074 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007729737.1" /locus_tag="PXH69_RS25595" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_25600" /product="hypothetical protein" /protein_id="WP_054781331.1" /transl_table=11 /translation="MEPLEPMRPVSVPADTHKSTPIWKSAPVTLGTVGVFAYALMSART GVVEVALGAAIAIAGAALYCMSVMRTIRENSCSRVPLLGTPPVSPRDVDLLAGAGMPLI MGGSLLAIRAAGWTWPYLYLGLLAVIMVATYVLPVVVHNRRLRKRTHRSQTP" gene complement(2157..3656) /locus_tag="PXH69_RS25600" /old_locus_tag="PXH69_25605" CDS complement(2157..3656) /EC_number="1.14.13.-" /GO_function="GO:0050660 - flavin adenine dinucleotide binding [Evidence IEA]" /GO_function="GO:0050661 - NADP binding [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) Pyr_redox_2" /gene_functions="other (smcogs) SMCOG1092: hypothetical protein" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019745606.1" /locus_tag="PXH69_RS25600" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_25605" /product="NAD(P)/FAD-dependent oxidoreductase" /protein_id="WP_003940522.1" /sec_met_domain="Pyr_redox_2 (E-value: 6.8e-10, bitscore: 31.9, seeds: 64, tool: rule-based-clusters)" /transl_table=11 /translation="MTQHVDVLIIGAGLSGIGAACHLIREQTGSTYAILERRENIGGTW DLFKYPGIRSDSDMLTFGFGFRPWIGTKVLADGASIRDYVEETAKEYGVTDHINFGRKV VAMDFDRATAQWSVTVLVEATGETETWTANVLVGACGYYNYDKGYRPAFPGEDDFRGQI VHPQHWPEDLDYTGKKVVVIGSGATAITLIPSMAPTAGHVTMLQRSPTWIQALPSEDPV AKGLKLARVPDQIAYKIGRARNIALQRASFQLSRTNPKLAKKLFLTQIRLQLGKNVDLR HFTPNYNPWDQRLCVVPNGDLFKVLKSGKADIVTDRIATFTEKGIVTESGREIEADVIV TATGLNVQILGGATMSIDGEPVKLNETVAYKSVLYSDIPNFLMILGYTNASWTLKADLA ASYLCRVLKIMRKRDYTTFEVHAEPEDFAEESLMGGALTSGYIQRGDGEMPRQGARGAW KVVNNYYRDRKLMHDAEIEDGVLQFSKVDIAVVPDSKVASA" gene 4173..4832 /locus_tag="PXH69_RS25605" /old_locus_tag="PXH69_25610" CDS 4173..4832 /GO_process="GO:0006355 - regulation of DNA-templated transcription [Evidence IEA]" /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007729739.1" /locus_tag="PXH69_RS25605" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_25610" /product="LuxR C-terminal-related transcriptional regulator" /protein_id="WP_063315605.1" /transl_table=11 /translation="MTLSITVTVYTTHSNRGFANAIAAGISARAYSEPQTADMSLLRHH TSDHGRALVLLPLGGVDWQVKELSALEGSVLVGDLSSGNSMIRGLAAGAHAVLNSQLPF TSLLDELQAAINPNSARSAHDKSSLVQRIRLRTEEFARISSLTDREFEIVGLLYGGSNV DSIARTAHIAPTTVRAHIRSILQKLGVHSQLEACAMLRRSGHDARRHPLELRIGRF" gene 5001..5888 /locus_tag="PXH69_RS25610" /old_locus_tag="PXH69_25615" CDS 5001..5888 /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) butyrolactone: AfsA" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007729740.1" /locus_tag="PXH69_RS25610" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_25615" /product="ScbA/BarX family gamma-butyrolactone biosynthesis protein" /protein_id="WP_063315604.1" /sec_met_domain="AfsA (E-value: 2.1e-19, bitscore: 62.8, seeds: 34, tool: rule-based-clusters)" /transl_table=11 /translation="MEYTRTVPRHLVHRHAVAEVFLTDYRIESDSAYQVAVQLPVSHRI FDVSSEQHDPLGVAEAFRQAVVLLCHTRYEIPLHYKFLMERFSFEMLDALDVQSVAEPL SFSMTVDNLTHRDGAVSGVEVSGILSEGNRHVARCNAVARGVSPEGYRRIRQGREDHRP SRRILPSGAAVVGSERVGRLTDGDVLISTDPARHAMFCAPDPRNHSLFDHPVDHIPGMI LFEAARQALRFHIGNPATKIASLSAVFPRFTEWETPCEVSTIPLDASGHVHRVRFTQEL ETTAELEITVADQS" gene complement(5927..6571) /locus_tag="PXH69_RS25615" /old_locus_tag="PXH69_25620" CDS complement(5927..6571) /codon_start=1 /gene_functions="regulatory (smcogs) SMCOG1057: TetR family transcriptional regulator" /gene_kind="regulatory" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007729741.1" /locus_tag="PXH69_RS25615" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_25620" /product="ScbR family autoregulator-binding transcription factor" /protein_id="WP_003940238.1" /transl_table=11 /translation="MQERAVLTRQAVILGAAKSFEKFGYSASLGTILQHGGVSKGAMYF HFASKEELAHAVIAAQHGMAMEGTRRVAAHSDIAVETLVLVSQEMARQLVTEPIARGGM RLTMEIGSIQGPIEQPYLDWIEAIKQVATQAAEAGDIVPGTDIDALARFVVGSFTGVQI LSEMLTGRTDLYERLSQMWNLVLPTIVREDKLAHVMQLAALAPREWEAPSA" gene 6658..7500 /locus_tag="PXH69_RS25620" /old_locus_tag="PXH69_25625" CDS 6658..7500 /GO_function="GO:0003824 - catalytic activity [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) RmlD_sub_bind" /gene_functions="biosynthetic-additional (rule-based-clusters) Polysacc_synt_2" /gene_functions="biosynthetic-additional (smcogs) SMCOG1010: NAD-dependent epimerase/dehydratase" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003940604.1" /locus_tag="PXH69_RS25620" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_25625" /product="NAD-dependent epimerase/dehydratase family protein" /protein_id="WP_030536480.1" /sec_met_domain="RmlD_sub_bind (E-value: 8.9e-14, bitscore: 44.5, seeds: 37, tool: rule-based-clusters)" /sec_met_domain="Polysacc_synt_2 (E-value: 1.6e-08, bitscore: 27.3, seeds: 77, tool: rule-based-clusters)" /transl_table=11 /translation="MTTLVLGATGFIGSAVMKQLVSRDGNAAIGFVRTATAAKQFGREG IATVIGDINESASLRLAMSQASTVICCVSYVGDDEQQCVHVNQHGVQNVASMAADVGVE RLLYVSTAAVYGPGPFRDLPVNGAPLSPFSPASRTRALAEKFVRDAGGLVVRPHLVYGP GDRWFLPTLTRIVSRLDSIIDGGSAVLSVVDVDLLARSIYELSIEQQFRYGSTLHVNEP VPRTVIDLLEQHARETNWTLPQNSIPRADAVKAAAQLSLDMHQIDMISLDHWFRSRLY" gene 7859..8977 /locus_tag="PXH69_RS25625" /old_locus_tag="PXH69_25630" CDS 7859..8977 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019745611.1" /locus_tag="PXH69_RS25625" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_25630" /product="hypothetical protein" /protein_id="WP_050656070.1" /transl_table=11 /translation="MLTADILLSGPRGRRMLLAYAIDAERALRPEPGDDMFRINAHRAA YHLDIGQGTSQVLFGPGAEEARQTIVTAEGVARSLTEISLPDVTAVDLRSALAEAVDAA RYWQDPDGEDVLVATEVMRDELRRVAEHIAGSECARWWSTSLASSDQWSILSRGEESPD TDEVPAAQQLLSEWRTSTLESEARAERDRPVDPAANWSGYWWSNPPTQGSTRALFDGTP AGLWFVEDSMGWERVVVRALRPPTGARIYEIDSAQAWSELCRRFPLEVTAETRQVWYRT TGRSGRWVIPDWVQVAERFEGVHLTVTGYLAAAGTAIEVDTETASVIAGWAPDETYWFT DAATYISEPCAWVLHDGPEVTDQPAWTLESQQ" misc_feature 9145..9160 /note="TFBS match to CopR, Copper-responsive regulator, confidence: weak, score: 21.62" /tool="antismash" gene 9197..9910 /locus_tag="PXH69_RS25630" /old_locus_tag="PXH69_25635" CDS 9197..9910 /codon_start=1 /gene_functions="regulatory (smcogs) SMCOG1071: GntR family transcriptional regulator" /gene_kind="regulatory" /inference="COORDINATES: protein motif:HMM:NF019349.4" /locus_tag="PXH69_RS25630" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_25635" /product="FCD domain-containing protein" /protein_id="WP_003940486.1" /transl_table=11 /translation="MPDSRIAPTARNIPLSVQIAEQLRSQITSGNWELGEKIPGEHQLV EMTGASRNTVREAIRGLVHAGLLEARPGDGTYVCASSELEIALQRRAAAENVLEIFEAR EALELYAARLAAERALPEDIDRMTRALDGRDAEPDVAKSVEYDLDFHLEMFAASGNQLI TDIYRSLDRSDTMSIPQGKSDKETRQLFIGPWTDDDPHRQLLAAIRDRDPDGAAAAVVR LMDHSRSLFLQNMPK" ORIGIN 1 ctcgtggacc gaggctgaac tgaccaagat cggcgacgcc gaggagttgc aggtctcctc 61 gtaccgccga gacggaaccg caaggccgta cgtcacgatt tgggttgtcc gtaccgacaa 121 ccaactgtac gttcgctcgg cctacggaac cgacaacccg tggttcgtcc gagcaacggc 181 aagtggaacg ggccgaatcc gagcaggagg aatcgagcgg gacgtggcgt tcgagtccgc 241 cgcaccggat gcccactcgt cgatcgactc cgcctatcac accaagtacg accgctacgg 301 cccgcagatc gtcggctccg ttaccggccg cgccgccgca cccaacacca ttcgacttgt 361 tccgctgacg cagaactgaa ccgccccttt cgattccgag gagacctcat ggaaatcttg 421 aagccccagc ccaccagcaa agcacccagc gactggttca ccggcgacgt ctggtgggac 481 gtcatctacg ccggccagga gccgtcgaag atgcgcgcca acatggttcg ctttgcaccg 541 tgcgcgcgaa ccgactggca ttcccacgcc ctcggacaga ccctccacat cgtctccggg 601 accgcgctgg ttcaagctcg cggcggcgag atcgtcgaag ttcatccggg cgagaccatc 661 tataccccgg caggagagga acactggcac ggcgcagcac ccgaccgttt catgacccac 721 cttgcgctgt gggaaggacc cggcggcgga agcaccgaaa ctacatgggg cgacaaagtc 781 accgatgagg aatacggcgg tccccgcgcc agacgtcgtt gagaccggac acctctcaga 841 tacggcgcat cacggaaaca actttgccca ggatgaccgc gcgatcgccg tcgatgacgc 901 tgtatgcggg attgcgaggc tcgaggtaca cgtgaccgtc gcgacgccgg agcaccttca 961 ccgtggcctc gccgtcgatc atcgcggcga cgatctcacc ggaatgagct tcgtcctggc 1021 ggcgcaccac gacgacgtct accgtcacag atcgctgcgt cgaccatcga ctcaccacgc 1081 acccgaagtc cgaacaccgt gccggagccg acgagctcgc gaggaagcgc aagcacctcg 1141 tcggcgtgct cctccgcgag gatcggcgcg ccggccgcga tgtcaccgac gaccggaacc 1201 gtcaccgtat tcgacgacga ctgttccttc gcgcctacga ggaacggccg cacgtccaac 1261 tgccgagcca tggccgctcc cctacggagg aagcccttct cttcgagact cttgaggtgc 1321 ttggacaccg tcgacgtcga gcgcagacca accgcgtctc cgatctgccg tgtgctcggc 1381 gtgcacccgt tggcagccac gagatcccgg atggtcatca gaatctgctg ctggcggagc 1441 ggcagggagg aggcgtcgag gccaccgaac atgtcgagat cgtcgtaatc ggtcacgatg 1501 agaatcgtag tgaggggtcc cgacatccag gccaggcagt cggagtctgc cagaaacggc 1561 acaggcagtt tgtttcccga gcggcgcatg gcacgctgag ctgatggagc ctctcgaacc 1621 gatgcgcccg gtcagtgttc ccgccgacac ccataagtcg acgccgatct ggaagtcggc 1681 accggtcact ttgggaacgg tgggtgtgtt tgcctacgca ttgatgtccg cgagaaccgg 1741 cgtcgtcgaa gttgcacttg gcgcggcgat cgcgatcgcc ggcgccgccc tctactgcat 1801 gtcagtgatg cgcacgatca gagaaaactc gtgcagccgc gtacctctgc tcggaacccc 1861 acccgtctcg ccccgtgacg tcgacctgct cgccggggcc gggatgccct tgatcatggg 1921 cggatcgttg ctggccatac gagcagcggg atggacctgg ccatatctct atctcggact 1981 gctcgccgtg atcatggtcg ctacctatgt tcttcccgtg gtggtgcaca accgacgatt 2041 acggaagcga actcaccgat cgcaaacccc gtgacgcggg tacgtcgacg caacatctcc 2101 agcacattct ccgacgtccc cactggtacg accggtccat cccccggtgg agccgactat 2161 gcgctggcga ccttgctatc aggcacgaca gcaatatcga ccttgctgaa ctgcagcaca 2221 ccgtcttcga tctcggcgtc gtgcatcagc ttgcggtcgc ggtagtaatt gttgaccact 2281 ttccacgcac cgcgggcacc ctgacgcggc atctctccgt cgccgcgctg gatgtagccc 2341 gaggtcaggg ctccgcccat gagagattct tcggcgaagt cctcaggttc ggcgtgaacc 2401 tcgaaagtcg tgtagtcgcg cttacgcatg atcttgagta cgcgacacag ataggacgcg 2461 gccaggtcgg ccttgagcgt ccaggatgcg ttggtgtagc cgaggatcat caggaagttc 2521 gggatgtcgg agtagagcac gctcttgtag gcgaccgtct cgttgagctt gacgggctcg 2581 ccgtcgatgc tcatggtcgc cccgcccagg atctgtacgt tcaagccggt cgccgtgacg 2641 atgacgtcgg cctcgatttc gcggcccgac tcggtcacga tgcccttctc ggtgaacgtg 2701 gcgatacggt cggtgacgat gtcggccttg ccgctcttga gcaccttgaa caggtccccg 2761 ttgggaacca cgcacaggcg ctgatcccac gggttgtagt tgggggtgaa gtgacgcagg 2821 tccacgttct tgccgagttg caagcggatc tgggtgagga acagcttctt cgccagcttc 2881 ggattggtgc gtgagagctg aaagctggcg cgttgcagtg cgatattgcg ggctcgaccg 2941 atcttgtaag cgatctgatc gggaacgcgt gcaagcttga gacccttggc cacagggtcc 3001 tcggacggta gcgcctggat ccacgtgggc gagcgctgca gcatggtgac gtgaccggca 3061 gtgggggcca tcgacgggat cagggtgatc gcggtggcac cggaaccgat gaccactacc 3121 ttctttccgg tgtaatcgag atcctccggc cagtgctgcg ggtgcacgat ctgaccgcgg 3181 aagtcgtcct caccggggaa ggcggggcgg tatcccttgt cgtagttgta gtaaccacag 3241 gcgccgacga ggacgttcgc ggtccacgtc tcggtctccc ccgtcgcctc gaccaggacg 3301 gtcacggacc actgtgcagt cgcgcgatcg aagtccattg ccacgacctt gcggccgaag 3361 ttgatgtggt cggtgacgcc gtattccttg gcggtttcct cgacatagtc acggatactg 3421 gcgccgtctg cgagcacttt ggttccgatc cacggacgga aaccgaatcc gaaggtgagc 3481 atgtcggaat ccgatcggat gccgggatac ttgaacaggt cccaggtgcc accgatgttc 3541 tcgcggcgtt cgaggatcgc gtaagtactt ccggtctgct cacgaatgag gtggcaagcc 3601 gcgccgattc cggacaagcc agcgccgatg atcagtacgt cgacatgctg tgtcatgagg 3661 aaactcgttt cgtcggtcgt cgcgaagcta cttctggcgt cggcagtgac cgagaacgat 3721 cggccggttc aggccgggtt ccctaaggtc agcgccaatg aacagggtat gtgcaactga 3781 cggtaacagt caatatctcg acccggccgc gcacggaaca ttgaggcgcg acgcaccggc 3841 ctcgcggaac tcgatcgaga cgcctgaatg cctcgagaca ccggagagtt gttcacgtcg 3901 tcgacggaac gcccaatgac tcgtctacac gggatactca tcgcccaaat attgagcgcc 3961 ccagcataat tcgaaggtcc atgatcaggt gtcgaacgcg cgagtttcca tgggaagacg 4021 ggcaactgcg atatcgccgt aaccttcgtg cgcatcagcg gaaaggtcac tgcgatagct 4081 aacctgctga cgaaaccacg ctgaaagcaa cggttttggt ttcgcagcga agagagcggg 4141 cggagcggac ctccaccgga gatgtagtcg ctatgacact ctccatcaca gtcaccgtgt 4201 acaccactca tagcaatcga gggttcgcca atgcaatcgc tgccggaatc agcgccaggg 4261 cttattccga gccacaaacc gcagacatga gcctgttaag gcatcacacc agcgatcacg 4321 gtagagcgct cgttctccta ccactcgggg gcgtcgattg gcaggtgaag gagctctccg 4381 cgctcgaggg atcggtcctg gtcggcgatc tcagtagcgg caactccatg attcgggggc 4441 tcgcagcggg cgcccacgcc gtgctcaact ctcaactgcc attcacatcc ctgctcgatg 4501 agctgcaggc cgcgatcaac cccaactccg ctcggtctgc acacgacaag tcctcgctcg 4561 tacaacgcat ccggttgcgc accgaagaat tcgcacgaat cagcagtctc accgatcgcg 4621 agttcgagat cgtgggcctt ctctacggag gctcgaatgt cgactcgatt gctcgaaccg 4681 cccacatcgc accgaccacg gttcgcgccc acatccgaag catcctgcaa aaactggggg 4741 tgcattccca gctggaggca tgcgcgatgc tccgccgctc tggtcacgac gcacgcagac 4801 acccactgga acttcggatc ggacgcttct agcaggaagt gccccagaca cactcgccga 4861 gatggggtgc cgcccctgat cgaactcgac cttcgaaaag atcgtcaatt ttgacgattc 4921 gatgacgcgc ccggacgtcg aatattaggt cctacacgac accgaagaca cgagtgaaca 4981 tcgaaaggtg cattcaatca atggaataca cacgcacagt gccgcggcat cttgtccaca 5041 gacatgctgt tgccgaggtt ttcttgacgg actaccggat cgaatcggac tcggcgtacc 5101 aggttgctgt gcaactcccg gtctcgcacc ggatattcga cgtatcgagc gagcaacacg 5161 atccgctcgg ggtggcagaa gcatttcgac aggcagtggt gcttctgtgc cacacccgat 5221 acgagattcc actgcactac aagttcctca tggaaaggtt cagcttcgag atgctggacg 5281 cgctcgacgt gcaatcagtc gcggagccgt tgagtttctc gatgacagtg gacaacctca 5341 ctcatcgcga cggagcggtt tcgggggtcg aagtgtcggg gatcctcagc gagggcaacc 5401 ggcacgtcgc cagatgcaac gctgttgcgc gaggagtcag ccccgaggga taccgtcgaa 5461 ttcggcaagg acgagaagac caccgcccga gccggcgcat ccttccttcc ggagccgcgg 5521 tggtcggatc ggagcgagtc ggacgcctaa ccgatggaga tgtgctcatc agcactgatc 5581 ctgctcggca cgcgatgttc tgcgctcccg atccgcgcaa ccactccctt ttcgaccacc 5641 ccgtcgacca catcccagga atgatacttt tcgaggccgc ccgacaagcg ctccgatttc 5701 acatcggcaa cccagcaaca aagatcgcct cactctcggc ggtctttccg aggttcactg 5761 aatgggaaac gccgtgcgag gtgtcaacga tcccccttga cgcgtcgggc catgtgcacc 5821 gagttcggtt cactcaagaa ctggaaacca ccgcggaact cgaaattaca gttgcggacc 5881 aatcatgaga gggaacgaac acgttcttct cgtcgcgtct tccacgtcaa gcgctcggtg 5941 cttcccactc tctcggtgca agcgccgcca gttgcatgac gtgcgccaac ttgtcctcgc 6001 ggacgatcgt tggcagcacc aggttccaca tctgactcaa ccgctcgtac agatcggttc 6061 tgcccgtcag catttccgag agaatctgca ctccggtgaa cgaccccacc acaaatcgag 6121 ccaacgcgtc tatatcggtg cccgggacga tatcgccggc ttcggccgct tgggttgcca 6181 cctgcttgat agcctcgatc caatcgaggt acggctgttc gatggggcct tgaatactgc 6241 cgatctccat cgtcaaccgc atgccacctc gcgcgatggg ctcggtcaca agctgccgag 6301 ccatctcctg cgaaacgagc accagggttt cgaccgcgat gtcggagtgc gcagcaaccc 6361 gccgggtacc ctccatcgcc ataccgtgct gcgctgcgat gaccgcgtgc gcaagctcct 6421 ccttggacgc gaaatgaaaa tacatcgccc ccttcgacac acccccgtgc tgcaagatcg 6481 tgcccaagct ggcggagtac ccgaacttct cgaagctctt cgcagcaccc aaaatgacgg 6541 cctggcgcgt caggacagca cgctcctgca tgatctaccc agctctcgtt gccgacggaa 6601 tgaatctcaa cgatacgtcg aaagagcccg cagcaacgct ctcgacacgg acacaccgtg 6661 acgactctgg tactcggggc cactggtttc atcggctccg cagtgatgaa acagctggtt 6721 tcccgcgacg gaaacgctgc aatcggtttc gttcgcacgg ccaccgcagc caaacagttc 6781 ggccgtgaag gaatcgccac cgtgatcggc gacatcaacg aaagtgcttc tctacgactt 6841 gcaatgagtc aggcgtcgac cgtcatctgt tgcgtgtcgt acgtgggcga cgacgaacaa 6901 cagtgcgttc acgtcaacca acacggcgta cagaacgttg cgtcgatggc cgccgacgtc 6961 ggcgtcgaac gcttgctcta cgtcagtacc gcggccgtgt acgggccggg accgtttcga 7021 gacttgccgg tcaacggcgc acctctcagc cccttctcac ctgccagtcg cacgcgcgct 7081 ctcgccgaga agttcgtgcg cgacgccgga ggcctggtcg tccggccgca tctcgtgtac 7141 ggacccggcg acagatggtt tcttccgaca ctgacgcgaa ttgtctctcg cctcgactcg 7201 atcatcgacg gcggtagcgc tgtcctgtcg gtagtcgacg tagacctact cgcccgatcc 7261 atctacgagt tgtcgataga acagcaattt cgctacggct caacacttca cgtcaacgag 7321 cctgtcccgc ggacagtcat cgaccttctc gagcaacatg cacgggagac caactggact 7381 ctgccccaga actctatccc ccgcgccgac gcggtgaaag ctgctgccca gctgagtctc 7441 gacatgcacc agatcgacat gatcagcctc gatcactggt tccggtcgag actgtactga 7501 tggcacacgg atcactcctg ccaaaacacc tgtctcccaa ctttttcagc cctctctatt 7561 gttcagcctc agcaatcaat acgacctatt tcgcagtcac tacaggcaaa cagagagctc 7621 tgatcgacga aaaccacagc tacgcaaaga cggcagtgat cgcgcacacc aaagaagcgg 7681 cgttcacgcg actggcagca gcctggggct cccttccgtc cgccttcgcc gccgaatgca 7741 gtgtgtggcg atcatgtcgt gcgcgacggg accgtcggga tttccgacat caaaggagaa 7801 agttctccgt ctcaacgacc tcggctaccg tcatctgaac cagaaaacgg gagaacagat 7861 gctcactgcg gatattttgc tgtcgggccc gcgcggacgc cggatgctgc tcgcgtatgc 7921 gatcgacgcc gaacgcgccc tgcgtcccga gccaggcgac gacatgttcc gcatcaacgc 7981 tcaccgggcg gcgtatcacc tcgacatcgg acaaggaacc tcccaagtgt tgttcggccc 8041 gggcgccgaa gaggcccggc agaccatagt caccgcggag ggcgtggcgc gcagcctcac 8101 ggaaatatcg ctccccgacg tcaccgccgt cgatctacgc tccgccctcg cagaagcggt 8161 cgatgcggcg cgttactggc aggatcccga cggagaagac gtcctcgtcg ccaccgaagt 8221 tatgcgagat gagcttcgac gcgtcgccga gcacatcgcc ggctccgaat gcgcgcggtg 8281 gtggtcaacc tcgctcgcct catccgacca atggtcgatt ctgagcagag gtgaggaatc 8341 tccggacaca gacgaagtgc ctgctgcgca acaactcctc agcgagtggc gcacgagcac 8401 cctggaatcg gaagcgagag ctgaacgtga ccgcccggtg gaccccgcgg cgaactggtc 8461 aggttattgg tggtcgaatc cacccactca gggttcgacg agggcgctgt tcgacgggac 8521 tccggcgggc ttgtggttcg tcgaagacag catgggatgg gaacgggtcg tcgtccgagc 8581 attgcggcca ccgactggcg cacgcatcta cgagatcgac agcgcgcaag cctggtcgga 8641 gctgtgcaga cggttcccgc tcgaggtcac cgcagaaacg cggcaggttt ggtatcggac 8701 taccggccga tcaggcaggt gggtcatccc cgactgggtg caggtcgcgg aaagatttga 8761 gggagtgcac cttaccgtga cgggatacct cgcggcagca ggaaccgcga tcgaggtcga 8821 taccgagacg gcgagcgtca tcgcgggctg ggcacccgac gaaacatact ggttcaccga 8881 tgctgccacc tacatttccg agccgtgtgc ttgggtgctg cacgacggcc cggaagtgac 8941 cgaccagccc gcatggacgc tggaatcgca gcaatgaccc gcacgtgagg gtctgctagg 9001 catgaggttc cgtgaggaat cgatcgcagt tgcccttcga gtcgagtcaa ctgccccgtg 9061 cgcggtcggc aggagcactc acgtgcacgc agacatgcgg aagggcggta ccccgcccag 9121 gccgatccga acccgagtgc cctagatcac aaaggtaaac ctatccacaa atcgccaaat 9181 gtcctacatt tgactcatgc cggattcccg tatcgcaccc accgcacgca acatcccact 9241 ttcagtgcag atcgcggaac aactgcggtc ccagatcact tctggcaact gggaattagg 9301 ggagaagatc cccggcgagc atcaacttgt cgaaatgacc ggcgcgagcc gcaatacggt 9361 ccgggaggcc attcgcggcc tcgtccatgc cggcctcctc gaagcacgcc cgggagacgg 9421 aacctacgtc tgcgcgagct cagaactcga aattgcactt caaagacgtg cagcggcgga 9481 gaacgtgctc gaaatcttcg aagcccgtga agctttggag ctctacgccg cccgactcgc 9541 agccgaacga gccctgcccg aagacatcga ccgaatgact cgggctttgg atggccgaga 9601 cgctgagccc gacgtcgcca aatccgtcga gtacgacctc gacttccacc tggagatgtt 9661 cgcagcgagc gggaaccaac tgatcacgga catctaccgc agtctcgatc gctctgacac 9721 catgagcatc ccacaaggta agtccgacaa ggagactcga cagctgttca tcggaccttg 9781 gaccgacgac gacccacacc ggcagctgct tgcggccatc cgtgaccgcg atccggacgg 9841 cgcagccgcc gcagtggtcc gactgatgga ccacagccga tccctctttc tgcaaaacat 9901 gccgaagtag ctgacacaca agaggttcag cgcacagaca atctcagaag ggcacaccta 9961 tggctaacgt catctccgac accgaaaacg tggcgtctgc cccgagcgcg ataccgataa 10021 tgcgccgagg actcgttatc gctggcattc ttctcatcgc agccaatctt cgagcctcaa 10081 tcacagtcgt cggcccgcta ctcggtgagg tacgcaccga tctcgatatc tcgtcgacaa 10141 cagcatcagc gctgatcagc cttccactgc tgtgcttcgc agtgttctcc cccgttgccc 10201 cggtcatcgc agctcggtgg ggaatggaac ggacactcgc agcagcactc ggcatgctcg 10261 ccttagcgat cgtgatccgc tctgttccgt ggatgcctgc gctgtggatc ggtaccgtat 10321 cgctcggcat gtccatcgca acaatgaacg ttctactgcc ggcgctcctc aaacgagact 10381 tcccgaccga agccggccgt ttgaccggca tcttctcggc ggtctctgca gccgtcgccg 10441 caaccgcatc cggacttgcc gtgccgatcg ccggtctcac cgatcacggt tggcggatcg 10501 cattcggaat ctgggctgct atggctctca taggatttgg ggtgttcctt ccccaacttc 10561 gccgacgcac gcttccgaaa cacgatcatg ctgcggcttt ggattcccat ccaggcggcc 10621 atcgctcccc ctggcgctca gcactcgggt ggcagatcac aatattcatg ggcgtccaat 10681 ctctcttctt ctacaccgtt ctgacatggt ggcccaccat tgaagaatca cacggcacta 10741 ccgcggctac cgccggactg catcaaggca tcatgcaaat attctcgatc gtcggcagcc 10801 tcctcacagc agtgctcctg cacaaactgc ccaaggatca gagaggagcc gtcctcgggc 10861 tggtgtcggt cgccgcagtg gcagttct //