LOCUS       NZ_JARDXE010000018     10888 bp    DNA     linear   CON 01-APR-2024
DEFINITION  Rhodococcus qingshengii strain HQ HQ1_scaffold18, whole genome
            shotgun sequence.
ACCESSION   NZ_JARDXE010000018
VERSION     NZ_JARDXE010000018.1
KEYWORDS    WGS; RefSeq.
SOURCE      Rhodococcus qingshengii
  ORGANISM  Rhodococcus qingshengii
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Nocardiaceae; Rhodococcus; Rhodococcus erythropolis group.
REFERENCE   1  (bases 1 to 163268)
  AUTHORS   Hu,J. and Xu,J.
  TITLE     A novel hydrolase synthesized by Rhodococcus erythropolis HQ is
            responsible for the detoxification of Zearalenone
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 163268)
  AUTHORS   Hu,J. and Xu,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-FEB-2023) Grain and Oil Risk Assessment Laboratory,
            Jiangsu Academy of Agricultural Sciences, No. 50, Zhongling Street,
            nanjing 210014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2022
            Assembly Method                   :: ABySS v. 2.1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: GCA_002079265.1
            Genome Coverage                   :: 470.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029076895.1-RS_2024_04_01
            Annotation Date                   :: 04/01/2024 06:35:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,927
            CDSs (total)                      :: 6,855
            Genes (coding)                    :: 6,768
            CDSs (with protein)               :: 6,768
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 63 of 87
            Pseudo Genes (internal stop)      :: 12 of 87
            Pseudo Genes (multiple problems)  :: 18 of 87
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:34:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 110872
            Orig. end                         :: 121760
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JARDXE010000018.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10888
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10888
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10888
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5888](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5888
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            409..822
                     /locus_tag="PXH69_RS25585"
                     /old_locus_tag="PXH69_25590"
     CDS             409..822
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003939933.1"
                     /locus_tag="PXH69_RS25585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_25590"
                     /product="cupin domain-containing protein"
                     /protein_id="WP_058226656.1"
                     /transl_table=11
                     /translation="MEILKPQPTSKAPSDWFTGDVWWDVIYAGQEPSKMRANMVRFAPC
                     ARTDWHSHALGQTLHIVSGTALVQARGGEIVEVHPGETIYTPAGEEHWHGAAPDRFMTH
                     LALWEGPGGGSTETTWGDKVTDEEYGGPRARRR"
     gene            complement(836..1526)
                     /gene="lexA"
                     /locus_tag="PXH69_RS25590"
                     /old_locus_tag="PXH69_25595"
                     /pseudo=""
     CDS             complement(836..1526)
                     /GO_function="GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene="lexA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003940361.1"
                     /locus_tag="PXH69_RS25590"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_25595"
                     /product="transcriptional repressor LexA"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSGPLTTILIVTDYDDLDMFGGLDASSLPLRQQQILMTIRDLVAA
                     NGCTPSTRQIGDAVGLRSTSTVSKHLKSLEEKGFLRRGAAMARQLDVRPFLVGAKEQSS
                     SNTVTVPVVGDIAAGAPILAEEHADEVLALPRELVGSGTVFGLRVRGESMVDAAICDGR
                     RRRGAPPGRSSFR"
     gene            1604..2074
                     /locus_tag="PXH69_RS25595"
                     /old_locus_tag="PXH69_25600"
     CDS             1604..2074
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007729737.1"
                     /locus_tag="PXH69_RS25595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_25600"
                     /product="hypothetical protein"
                     /protein_id="WP_054781331.1"
                     /transl_table=11
                     /translation="MEPLEPMRPVSVPADTHKSTPIWKSAPVTLGTVGVFAYALMSART
                     GVVEVALGAAIAIAGAALYCMSVMRTIRENSCSRVPLLGTPPVSPRDVDLLAGAGMPLI
                     MGGSLLAIRAAGWTWPYLYLGLLAVIMVATYVLPVVVHNRRLRKRTHRSQTP"
     gene            complement(2157..3656)
                     /locus_tag="PXH69_RS25600"
                     /old_locus_tag="PXH69_25605"
     CDS             complement(2157..3656)
                     /EC_number="1.14.13.-"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="other (smcogs) SMCOG1092: hypothetical
                     protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019745606.1"
                     /locus_tag="PXH69_RS25600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_25605"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_003940522.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 6.8e-10, bitscore:
                     31.9, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQHVDVLIIGAGLSGIGAACHLIREQTGSTYAILERRENIGGTW
                     DLFKYPGIRSDSDMLTFGFGFRPWIGTKVLADGASIRDYVEETAKEYGVTDHINFGRKV
                     VAMDFDRATAQWSVTVLVEATGETETWTANVLVGACGYYNYDKGYRPAFPGEDDFRGQI
                     VHPQHWPEDLDYTGKKVVVIGSGATAITLIPSMAPTAGHVTMLQRSPTWIQALPSEDPV
                     AKGLKLARVPDQIAYKIGRARNIALQRASFQLSRTNPKLAKKLFLTQIRLQLGKNVDLR
                     HFTPNYNPWDQRLCVVPNGDLFKVLKSGKADIVTDRIATFTEKGIVTESGREIEADVIV
                     TATGLNVQILGGATMSIDGEPVKLNETVAYKSVLYSDIPNFLMILGYTNASWTLKADLA
                     ASYLCRVLKIMRKRDYTTFEVHAEPEDFAEESLMGGALTSGYIQRGDGEMPRQGARGAW
                     KVVNNYYRDRKLMHDAEIEDGVLQFSKVDIAVVPDSKVASA"
     gene            4173..4832
                     /locus_tag="PXH69_RS25605"
                     /old_locus_tag="PXH69_25610"
     CDS             4173..4832
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007729739.1"
                     /locus_tag="PXH69_RS25605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_25610"
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="WP_063315605.1"
                     /transl_table=11
                     /translation="MTLSITVTVYTTHSNRGFANAIAAGISARAYSEPQTADMSLLRHH
                     TSDHGRALVLLPLGGVDWQVKELSALEGSVLVGDLSSGNSMIRGLAAGAHAVLNSQLPF
                     TSLLDELQAAINPNSARSAHDKSSLVQRIRLRTEEFARISSLTDREFEIVGLLYGGSNV
                     DSIARTAHIAPTTVRAHIRSILQKLGVHSQLEACAMLRRSGHDARRHPLELRIGRF"
     gene            5001..5888
                     /locus_tag="PXH69_RS25610"
                     /old_locus_tag="PXH69_25615"
     CDS             5001..5888
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007729740.1"
                     /locus_tag="PXH69_RS25610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_25615"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_063315604.1"
                     /sec_met_domain="AfsA (E-value: 2.1e-19, bitscore: 62.8,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEYTRTVPRHLVHRHAVAEVFLTDYRIESDSAYQVAVQLPVSHRI
                     FDVSSEQHDPLGVAEAFRQAVVLLCHTRYEIPLHYKFLMERFSFEMLDALDVQSVAEPL
                     SFSMTVDNLTHRDGAVSGVEVSGILSEGNRHVARCNAVARGVSPEGYRRIRQGREDHRP
                     SRRILPSGAAVVGSERVGRLTDGDVLISTDPARHAMFCAPDPRNHSLFDHPVDHIPGMI
                     LFEAARQALRFHIGNPATKIASLSAVFPRFTEWETPCEVSTIPLDASGHVHRVRFTQEL
                     ETTAELEITVADQS"
     gene            complement(5927..6571)
                     /locus_tag="PXH69_RS25615"
                     /old_locus_tag="PXH69_25620"
     CDS             complement(5927..6571)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007729741.1"
                     /locus_tag="PXH69_RS25615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_25620"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_003940238.1"
                     /transl_table=11
                     /translation="MQERAVLTRQAVILGAAKSFEKFGYSASLGTILQHGGVSKGAMYF
                     HFASKEELAHAVIAAQHGMAMEGTRRVAAHSDIAVETLVLVSQEMARQLVTEPIARGGM
                     RLTMEIGSIQGPIEQPYLDWIEAIKQVATQAAEAGDIVPGTDIDALARFVVGSFTGVQI
                     LSEMLTGRTDLYERLSQMWNLVLPTIVREDKLAHVMQLAALAPREWEAPSA"
     gene            6658..7500
                     /locus_tag="PXH69_RS25620"
                     /old_locus_tag="PXH69_25625"
     CDS             6658..7500
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003940604.1"
                     /locus_tag="PXH69_RS25620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_25625"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_030536480.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 8.9e-14, bitscore:
                     44.5, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 1.6e-08,
                     bitscore: 27.3, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTLVLGATGFIGSAVMKQLVSRDGNAAIGFVRTATAAKQFGREG
                     IATVIGDINESASLRLAMSQASTVICCVSYVGDDEQQCVHVNQHGVQNVASMAADVGVE
                     RLLYVSTAAVYGPGPFRDLPVNGAPLSPFSPASRTRALAEKFVRDAGGLVVRPHLVYGP
                     GDRWFLPTLTRIVSRLDSIIDGGSAVLSVVDVDLLARSIYELSIEQQFRYGSTLHVNEP
                     VPRTVIDLLEQHARETNWTLPQNSIPRADAVKAAAQLSLDMHQIDMISLDHWFRSRLY"
     gene            7859..8977
                     /locus_tag="PXH69_RS25625"
                     /old_locus_tag="PXH69_25630"
     CDS             7859..8977
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019745611.1"
                     /locus_tag="PXH69_RS25625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_25630"
                     /product="hypothetical protein"
                     /protein_id="WP_050656070.1"
                     /transl_table=11
                     /translation="MLTADILLSGPRGRRMLLAYAIDAERALRPEPGDDMFRINAHRAA
                     YHLDIGQGTSQVLFGPGAEEARQTIVTAEGVARSLTEISLPDVTAVDLRSALAEAVDAA
                     RYWQDPDGEDVLVATEVMRDELRRVAEHIAGSECARWWSTSLASSDQWSILSRGEESPD
                     TDEVPAAQQLLSEWRTSTLESEARAERDRPVDPAANWSGYWWSNPPTQGSTRALFDGTP
                     AGLWFVEDSMGWERVVVRALRPPTGARIYEIDSAQAWSELCRRFPLEVTAETRQVWYRT
                     TGRSGRWVIPDWVQVAERFEGVHLTVTGYLAAAGTAIEVDTETASVIAGWAPDETYWFT
                     DAATYISEPCAWVLHDGPEVTDQPAWTLESQQ"
     misc_feature    9145..9160
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 21.62"
                     /tool="antismash"
     gene            9197..9910
                     /locus_tag="PXH69_RS25630"
                     /old_locus_tag="PXH69_25635"
     CDS             9197..9910
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /locus_tag="PXH69_RS25630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_25635"
                     /product="FCD domain-containing protein"
                     /protein_id="WP_003940486.1"
                     /transl_table=11
                     /translation="MPDSRIAPTARNIPLSVQIAEQLRSQITSGNWELGEKIPGEHQLV
                     EMTGASRNTVREAIRGLVHAGLLEARPGDGTYVCASSELEIALQRRAAAENVLEIFEAR
                     EALELYAARLAAERALPEDIDRMTRALDGRDAEPDVAKSVEYDLDFHLEMFAASGNQLI
                     TDIYRSLDRSDTMSIPQGKSDKETRQLFIGPWTDDDPHRQLLAAIRDRDPDGAAAAVVR
                     LMDHSRSLFLQNMPK"
ORIGIN
        1 ctcgtggacc gaggctgaac tgaccaagat cggcgacgcc gaggagttgc aggtctcctc
       61 gtaccgccga gacggaaccg caaggccgta cgtcacgatt tgggttgtcc gtaccgacaa
      121 ccaactgtac gttcgctcgg cctacggaac cgacaacccg tggttcgtcc gagcaacggc
      181 aagtggaacg ggccgaatcc gagcaggagg aatcgagcgg gacgtggcgt tcgagtccgc
      241 cgcaccggat gcccactcgt cgatcgactc cgcctatcac accaagtacg accgctacgg
      301 cccgcagatc gtcggctccg ttaccggccg cgccgccgca cccaacacca ttcgacttgt
      361 tccgctgacg cagaactgaa ccgccccttt cgattccgag gagacctcat ggaaatcttg
      421 aagccccagc ccaccagcaa agcacccagc gactggttca ccggcgacgt ctggtgggac
      481 gtcatctacg ccggccagga gccgtcgaag atgcgcgcca acatggttcg ctttgcaccg
      541 tgcgcgcgaa ccgactggca ttcccacgcc ctcggacaga ccctccacat cgtctccggg
      601 accgcgctgg ttcaagctcg cggcggcgag atcgtcgaag ttcatccggg cgagaccatc
      661 tataccccgg caggagagga acactggcac ggcgcagcac ccgaccgttt catgacccac
      721 cttgcgctgt gggaaggacc cggcggcgga agcaccgaaa ctacatgggg cgacaaagtc
      781 accgatgagg aatacggcgg tccccgcgcc agacgtcgtt gagaccggac acctctcaga
      841 tacggcgcat cacggaaaca actttgccca ggatgaccgc gcgatcgccg tcgatgacgc
      901 tgtatgcggg attgcgaggc tcgaggtaca cgtgaccgtc gcgacgccgg agcaccttca
      961 ccgtggcctc gccgtcgatc atcgcggcga cgatctcacc ggaatgagct tcgtcctggc
     1021 ggcgcaccac gacgacgtct accgtcacag atcgctgcgt cgaccatcga ctcaccacgc
     1081 acccgaagtc cgaacaccgt gccggagccg acgagctcgc gaggaagcgc aagcacctcg
     1141 tcggcgtgct cctccgcgag gatcggcgcg ccggccgcga tgtcaccgac gaccggaacc
     1201 gtcaccgtat tcgacgacga ctgttccttc gcgcctacga ggaacggccg cacgtccaac
     1261 tgccgagcca tggccgctcc cctacggagg aagcccttct cttcgagact cttgaggtgc
     1321 ttggacaccg tcgacgtcga gcgcagacca accgcgtctc cgatctgccg tgtgctcggc
     1381 gtgcacccgt tggcagccac gagatcccgg atggtcatca gaatctgctg ctggcggagc
     1441 ggcagggagg aggcgtcgag gccaccgaac atgtcgagat cgtcgtaatc ggtcacgatg
     1501 agaatcgtag tgaggggtcc cgacatccag gccaggcagt cggagtctgc cagaaacggc
     1561 acaggcagtt tgtttcccga gcggcgcatg gcacgctgag ctgatggagc ctctcgaacc
     1621 gatgcgcccg gtcagtgttc ccgccgacac ccataagtcg acgccgatct ggaagtcggc
     1681 accggtcact ttgggaacgg tgggtgtgtt tgcctacgca ttgatgtccg cgagaaccgg
     1741 cgtcgtcgaa gttgcacttg gcgcggcgat cgcgatcgcc ggcgccgccc tctactgcat
     1801 gtcagtgatg cgcacgatca gagaaaactc gtgcagccgc gtacctctgc tcggaacccc
     1861 acccgtctcg ccccgtgacg tcgacctgct cgccggggcc gggatgccct tgatcatggg
     1921 cggatcgttg ctggccatac gagcagcggg atggacctgg ccatatctct atctcggact
     1981 gctcgccgtg atcatggtcg ctacctatgt tcttcccgtg gtggtgcaca accgacgatt
     2041 acggaagcga actcaccgat cgcaaacccc gtgacgcggg tacgtcgacg caacatctcc
     2101 agcacattct ccgacgtccc cactggtacg accggtccat cccccggtgg agccgactat
     2161 gcgctggcga ccttgctatc aggcacgaca gcaatatcga ccttgctgaa ctgcagcaca
     2221 ccgtcttcga tctcggcgtc gtgcatcagc ttgcggtcgc ggtagtaatt gttgaccact
     2281 ttccacgcac cgcgggcacc ctgacgcggc atctctccgt cgccgcgctg gatgtagccc
     2341 gaggtcaggg ctccgcccat gagagattct tcggcgaagt cctcaggttc ggcgtgaacc
     2401 tcgaaagtcg tgtagtcgcg cttacgcatg atcttgagta cgcgacacag ataggacgcg
     2461 gccaggtcgg ccttgagcgt ccaggatgcg ttggtgtagc cgaggatcat caggaagttc
     2521 gggatgtcgg agtagagcac gctcttgtag gcgaccgtct cgttgagctt gacgggctcg
     2581 ccgtcgatgc tcatggtcgc cccgcccagg atctgtacgt tcaagccggt cgccgtgacg
     2641 atgacgtcgg cctcgatttc gcggcccgac tcggtcacga tgcccttctc ggtgaacgtg
     2701 gcgatacggt cggtgacgat gtcggccttg ccgctcttga gcaccttgaa caggtccccg
     2761 ttgggaacca cgcacaggcg ctgatcccac gggttgtagt tgggggtgaa gtgacgcagg
     2821 tccacgttct tgccgagttg caagcggatc tgggtgagga acagcttctt cgccagcttc
     2881 ggattggtgc gtgagagctg aaagctggcg cgttgcagtg cgatattgcg ggctcgaccg
     2941 atcttgtaag cgatctgatc gggaacgcgt gcaagcttga gacccttggc cacagggtcc
     3001 tcggacggta gcgcctggat ccacgtgggc gagcgctgca gcatggtgac gtgaccggca
     3061 gtgggggcca tcgacgggat cagggtgatc gcggtggcac cggaaccgat gaccactacc
     3121 ttctttccgg tgtaatcgag atcctccggc cagtgctgcg ggtgcacgat ctgaccgcgg
     3181 aagtcgtcct caccggggaa ggcggggcgg tatcccttgt cgtagttgta gtaaccacag
     3241 gcgccgacga ggacgttcgc ggtccacgtc tcggtctccc ccgtcgcctc gaccaggacg
     3301 gtcacggacc actgtgcagt cgcgcgatcg aagtccattg ccacgacctt gcggccgaag
     3361 ttgatgtggt cggtgacgcc gtattccttg gcggtttcct cgacatagtc acggatactg
     3421 gcgccgtctg cgagcacttt ggttccgatc cacggacgga aaccgaatcc gaaggtgagc
     3481 atgtcggaat ccgatcggat gccgggatac ttgaacaggt cccaggtgcc accgatgttc
     3541 tcgcggcgtt cgaggatcgc gtaagtactt ccggtctgct cacgaatgag gtggcaagcc
     3601 gcgccgattc cggacaagcc agcgccgatg atcagtacgt cgacatgctg tgtcatgagg
     3661 aaactcgttt cgtcggtcgt cgcgaagcta cttctggcgt cggcagtgac cgagaacgat
     3721 cggccggttc aggccgggtt ccctaaggtc agcgccaatg aacagggtat gtgcaactga
     3781 cggtaacagt caatatctcg acccggccgc gcacggaaca ttgaggcgcg acgcaccggc
     3841 ctcgcggaac tcgatcgaga cgcctgaatg cctcgagaca ccggagagtt gttcacgtcg
     3901 tcgacggaac gcccaatgac tcgtctacac gggatactca tcgcccaaat attgagcgcc
     3961 ccagcataat tcgaaggtcc atgatcaggt gtcgaacgcg cgagtttcca tgggaagacg
     4021 ggcaactgcg atatcgccgt aaccttcgtg cgcatcagcg gaaaggtcac tgcgatagct
     4081 aacctgctga cgaaaccacg ctgaaagcaa cggttttggt ttcgcagcga agagagcggg
     4141 cggagcggac ctccaccgga gatgtagtcg ctatgacact ctccatcaca gtcaccgtgt
     4201 acaccactca tagcaatcga gggttcgcca atgcaatcgc tgccggaatc agcgccaggg
     4261 cttattccga gccacaaacc gcagacatga gcctgttaag gcatcacacc agcgatcacg
     4321 gtagagcgct cgttctccta ccactcgggg gcgtcgattg gcaggtgaag gagctctccg
     4381 cgctcgaggg atcggtcctg gtcggcgatc tcagtagcgg caactccatg attcgggggc
     4441 tcgcagcggg cgcccacgcc gtgctcaact ctcaactgcc attcacatcc ctgctcgatg
     4501 agctgcaggc cgcgatcaac cccaactccg ctcggtctgc acacgacaag tcctcgctcg
     4561 tacaacgcat ccggttgcgc accgaagaat tcgcacgaat cagcagtctc accgatcgcg
     4621 agttcgagat cgtgggcctt ctctacggag gctcgaatgt cgactcgatt gctcgaaccg
     4681 cccacatcgc accgaccacg gttcgcgccc acatccgaag catcctgcaa aaactggggg
     4741 tgcattccca gctggaggca tgcgcgatgc tccgccgctc tggtcacgac gcacgcagac
     4801 acccactgga acttcggatc ggacgcttct agcaggaagt gccccagaca cactcgccga
     4861 gatggggtgc cgcccctgat cgaactcgac cttcgaaaag atcgtcaatt ttgacgattc
     4921 gatgacgcgc ccggacgtcg aatattaggt cctacacgac accgaagaca cgagtgaaca
     4981 tcgaaaggtg cattcaatca atggaataca cacgcacagt gccgcggcat cttgtccaca
     5041 gacatgctgt tgccgaggtt ttcttgacgg actaccggat cgaatcggac tcggcgtacc
     5101 aggttgctgt gcaactcccg gtctcgcacc ggatattcga cgtatcgagc gagcaacacg
     5161 atccgctcgg ggtggcagaa gcatttcgac aggcagtggt gcttctgtgc cacacccgat
     5221 acgagattcc actgcactac aagttcctca tggaaaggtt cagcttcgag atgctggacg
     5281 cgctcgacgt gcaatcagtc gcggagccgt tgagtttctc gatgacagtg gacaacctca
     5341 ctcatcgcga cggagcggtt tcgggggtcg aagtgtcggg gatcctcagc gagggcaacc
     5401 ggcacgtcgc cagatgcaac gctgttgcgc gaggagtcag ccccgaggga taccgtcgaa
     5461 ttcggcaagg acgagaagac caccgcccga gccggcgcat ccttccttcc ggagccgcgg
     5521 tggtcggatc ggagcgagtc ggacgcctaa ccgatggaga tgtgctcatc agcactgatc
     5581 ctgctcggca cgcgatgttc tgcgctcccg atccgcgcaa ccactccctt ttcgaccacc
     5641 ccgtcgacca catcccagga atgatacttt tcgaggccgc ccgacaagcg ctccgatttc
     5701 acatcggcaa cccagcaaca aagatcgcct cactctcggc ggtctttccg aggttcactg
     5761 aatgggaaac gccgtgcgag gtgtcaacga tcccccttga cgcgtcgggc catgtgcacc
     5821 gagttcggtt cactcaagaa ctggaaacca ccgcggaact cgaaattaca gttgcggacc
     5881 aatcatgaga gggaacgaac acgttcttct cgtcgcgtct tccacgtcaa gcgctcggtg
     5941 cttcccactc tctcggtgca agcgccgcca gttgcatgac gtgcgccaac ttgtcctcgc
     6001 ggacgatcgt tggcagcacc aggttccaca tctgactcaa ccgctcgtac agatcggttc
     6061 tgcccgtcag catttccgag agaatctgca ctccggtgaa cgaccccacc acaaatcgag
     6121 ccaacgcgtc tatatcggtg cccgggacga tatcgccggc ttcggccgct tgggttgcca
     6181 cctgcttgat agcctcgatc caatcgaggt acggctgttc gatggggcct tgaatactgc
     6241 cgatctccat cgtcaaccgc atgccacctc gcgcgatggg ctcggtcaca agctgccgag
     6301 ccatctcctg cgaaacgagc accagggttt cgaccgcgat gtcggagtgc gcagcaaccc
     6361 gccgggtacc ctccatcgcc ataccgtgct gcgctgcgat gaccgcgtgc gcaagctcct
     6421 ccttggacgc gaaatgaaaa tacatcgccc ccttcgacac acccccgtgc tgcaagatcg
     6481 tgcccaagct ggcggagtac ccgaacttct cgaagctctt cgcagcaccc aaaatgacgg
     6541 cctggcgcgt caggacagca cgctcctgca tgatctaccc agctctcgtt gccgacggaa
     6601 tgaatctcaa cgatacgtcg aaagagcccg cagcaacgct ctcgacacgg acacaccgtg
     6661 acgactctgg tactcggggc cactggtttc atcggctccg cagtgatgaa acagctggtt
     6721 tcccgcgacg gaaacgctgc aatcggtttc gttcgcacgg ccaccgcagc caaacagttc
     6781 ggccgtgaag gaatcgccac cgtgatcggc gacatcaacg aaagtgcttc tctacgactt
     6841 gcaatgagtc aggcgtcgac cgtcatctgt tgcgtgtcgt acgtgggcga cgacgaacaa
     6901 cagtgcgttc acgtcaacca acacggcgta cagaacgttg cgtcgatggc cgccgacgtc
     6961 ggcgtcgaac gcttgctcta cgtcagtacc gcggccgtgt acgggccggg accgtttcga
     7021 gacttgccgg tcaacggcgc acctctcagc cccttctcac ctgccagtcg cacgcgcgct
     7081 ctcgccgaga agttcgtgcg cgacgccgga ggcctggtcg tccggccgca tctcgtgtac
     7141 ggacccggcg acagatggtt tcttccgaca ctgacgcgaa ttgtctctcg cctcgactcg
     7201 atcatcgacg gcggtagcgc tgtcctgtcg gtagtcgacg tagacctact cgcccgatcc
     7261 atctacgagt tgtcgataga acagcaattt cgctacggct caacacttca cgtcaacgag
     7321 cctgtcccgc ggacagtcat cgaccttctc gagcaacatg cacgggagac caactggact
     7381 ctgccccaga actctatccc ccgcgccgac gcggtgaaag ctgctgccca gctgagtctc
     7441 gacatgcacc agatcgacat gatcagcctc gatcactggt tccggtcgag actgtactga
     7501 tggcacacgg atcactcctg ccaaaacacc tgtctcccaa ctttttcagc cctctctatt
     7561 gttcagcctc agcaatcaat acgacctatt tcgcagtcac tacaggcaaa cagagagctc
     7621 tgatcgacga aaaccacagc tacgcaaaga cggcagtgat cgcgcacacc aaagaagcgg
     7681 cgttcacgcg actggcagca gcctggggct cccttccgtc cgccttcgcc gccgaatgca
     7741 gtgtgtggcg atcatgtcgt gcgcgacggg accgtcggga tttccgacat caaaggagaa
     7801 agttctccgt ctcaacgacc tcggctaccg tcatctgaac cagaaaacgg gagaacagat
     7861 gctcactgcg gatattttgc tgtcgggccc gcgcggacgc cggatgctgc tcgcgtatgc
     7921 gatcgacgcc gaacgcgccc tgcgtcccga gccaggcgac gacatgttcc gcatcaacgc
     7981 tcaccgggcg gcgtatcacc tcgacatcgg acaaggaacc tcccaagtgt tgttcggccc
     8041 gggcgccgaa gaggcccggc agaccatagt caccgcggag ggcgtggcgc gcagcctcac
     8101 ggaaatatcg ctccccgacg tcaccgccgt cgatctacgc tccgccctcg cagaagcggt
     8161 cgatgcggcg cgttactggc aggatcccga cggagaagac gtcctcgtcg ccaccgaagt
     8221 tatgcgagat gagcttcgac gcgtcgccga gcacatcgcc ggctccgaat gcgcgcggtg
     8281 gtggtcaacc tcgctcgcct catccgacca atggtcgatt ctgagcagag gtgaggaatc
     8341 tccggacaca gacgaagtgc ctgctgcgca acaactcctc agcgagtggc gcacgagcac
     8401 cctggaatcg gaagcgagag ctgaacgtga ccgcccggtg gaccccgcgg cgaactggtc
     8461 aggttattgg tggtcgaatc cacccactca gggttcgacg agggcgctgt tcgacgggac
     8521 tccggcgggc ttgtggttcg tcgaagacag catgggatgg gaacgggtcg tcgtccgagc
     8581 attgcggcca ccgactggcg cacgcatcta cgagatcgac agcgcgcaag cctggtcgga
     8641 gctgtgcaga cggttcccgc tcgaggtcac cgcagaaacg cggcaggttt ggtatcggac
     8701 taccggccga tcaggcaggt gggtcatccc cgactgggtg caggtcgcgg aaagatttga
     8761 gggagtgcac cttaccgtga cgggatacct cgcggcagca ggaaccgcga tcgaggtcga
     8821 taccgagacg gcgagcgtca tcgcgggctg ggcacccgac gaaacatact ggttcaccga
     8881 tgctgccacc tacatttccg agccgtgtgc ttgggtgctg cacgacggcc cggaagtgac
     8941 cgaccagccc gcatggacgc tggaatcgca gcaatgaccc gcacgtgagg gtctgctagg
     9001 catgaggttc cgtgaggaat cgatcgcagt tgcccttcga gtcgagtcaa ctgccccgtg
     9061 cgcggtcggc aggagcactc acgtgcacgc agacatgcgg aagggcggta ccccgcccag
     9121 gccgatccga acccgagtgc cctagatcac aaaggtaaac ctatccacaa atcgccaaat
     9181 gtcctacatt tgactcatgc cggattcccg tatcgcaccc accgcacgca acatcccact
     9241 ttcagtgcag atcgcggaac aactgcggtc ccagatcact tctggcaact gggaattagg
     9301 ggagaagatc cccggcgagc atcaacttgt cgaaatgacc ggcgcgagcc gcaatacggt
     9361 ccgggaggcc attcgcggcc tcgtccatgc cggcctcctc gaagcacgcc cgggagacgg
     9421 aacctacgtc tgcgcgagct cagaactcga aattgcactt caaagacgtg cagcggcgga
     9481 gaacgtgctc gaaatcttcg aagcccgtga agctttggag ctctacgccg cccgactcgc
     9541 agccgaacga gccctgcccg aagacatcga ccgaatgact cgggctttgg atggccgaga
     9601 cgctgagccc gacgtcgcca aatccgtcga gtacgacctc gacttccacc tggagatgtt
     9661 cgcagcgagc gggaaccaac tgatcacgga catctaccgc agtctcgatc gctctgacac
     9721 catgagcatc ccacaaggta agtccgacaa ggagactcga cagctgttca tcggaccttg
     9781 gaccgacgac gacccacacc ggcagctgct tgcggccatc cgtgaccgcg atccggacgg
     9841 cgcagccgcc gcagtggtcc gactgatgga ccacagccga tccctctttc tgcaaaacat
     9901 gccgaagtag ctgacacaca agaggttcag cgcacagaca atctcagaag ggcacaccta
     9961 tggctaacgt catctccgac accgaaaacg tggcgtctgc cccgagcgcg ataccgataa
    10021 tgcgccgagg actcgttatc gctggcattc ttctcatcgc agccaatctt cgagcctcaa
    10081 tcacagtcgt cggcccgcta ctcggtgagg tacgcaccga tctcgatatc tcgtcgacaa
    10141 cagcatcagc gctgatcagc cttccactgc tgtgcttcgc agtgttctcc cccgttgccc
    10201 cggtcatcgc agctcggtgg ggaatggaac ggacactcgc agcagcactc ggcatgctcg
    10261 ccttagcgat cgtgatccgc tctgttccgt ggatgcctgc gctgtggatc ggtaccgtat
    10321 cgctcggcat gtccatcgca acaatgaacg ttctactgcc ggcgctcctc aaacgagact
    10381 tcccgaccga agccggccgt ttgaccggca tcttctcggc ggtctctgca gccgtcgccg
    10441 caaccgcatc cggacttgcc gtgccgatcg ccggtctcac cgatcacggt tggcggatcg
    10501 cattcggaat ctgggctgct atggctctca taggatttgg ggtgttcctt ccccaacttc
    10561 gccgacgcac gcttccgaaa cacgatcatg ctgcggcttt ggattcccat ccaggcggcc
    10621 atcgctcccc ctggcgctca gcactcgggt ggcagatcac aatattcatg ggcgtccaat
    10681 ctctcttctt ctacaccgtt ctgacatggt ggcccaccat tgaagaatca cacggcacta
    10741 ccgcggctac cgccggactg catcaaggca tcatgcaaat attctcgatc gtcggcagcc
    10801 tcctcacagc agtgctcctg cacaaactgc ccaaggatca gagaggagcc gtcctcgggc
    10861 tggtgtcggt cgccgcagtg gcagttct
//
