LOCUS       NZ_JARDXE010000012     10399 bp    DNA     linear   CON 01-APR-2024
DEFINITION  Rhodococcus qingshengii strain HQ HQ1_scaffold12, whole genome
            shotgun sequence.
ACCESSION   NZ_JARDXE010000012
VERSION     NZ_JARDXE010000012.1
KEYWORDS    WGS; RefSeq.
SOURCE      Rhodococcus qingshengii
  ORGANISM  Rhodococcus qingshengii
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Nocardiaceae; Rhodococcus; Rhodococcus erythropolis group.
REFERENCE   1  (bases 1 to 227923)
  AUTHORS   Hu,J. and Xu,J.
  TITLE     A novel hydrolase synthesized by Rhodococcus erythropolis HQ is
            responsible for the detoxification of Zearalenone
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 227923)
  AUTHORS   Hu,J. and Xu,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-FEB-2023) Grain and Oil Risk Assessment Laboratory,
            Jiangsu Academy of Agricultural Sciences, No. 50, Zhongling Street,
            nanjing 210014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2022
            Assembly Method                   :: ABySS v. 2.1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: GCA_002079265.1
            Genome Coverage                   :: 470.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029076895.1-RS_2024_04_01
            Annotation Date                   :: 04/01/2024 06:35:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,927
            CDSs (total)                      :: 6,855
            Genes (coding)                    :: 6,768
            CDSs (with protein)               :: 6,768
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 63 of 87
            Pseudo Genes (internal stop)      :: 12 of 87
            Pseudo Genes (multiple problems)  :: 18 of 87
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:34:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 53612
            Orig. end                         :: 64011
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JARDXE010000012.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            13..1464
                     /gene="obgE"
                     /locus_tag="PXH69_RS19210"
                     /old_locus_tag="PXH69_19205"
     CDS             13..1464
                     /GO_function="GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0003924 - GTPase activity [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /gene="obgE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019749068.1"
                     /locus_tag="PXH69_RS19210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_19205"
                     /product="GTPase ObgE"
                     /protein_id="WP_003944579.1"
                     /transl_table=11
                     /translation="MSRFIDRVVLHVSAGKGGNGCASVHREKFKPLGGPDGANGGRGGD
                     VILVVDENIHTLLDFHFHPNAKATNGKQGAGSNREGANGEDLILKVPDGTVVLDTDGNV
                     LADLVGVGSRFDAAQGGRGGLGNAALASKARKAPGFALLGEDGVERDLVLELKSVADVG
                     LLGFPSAGKSSLVSVLSAAKPKIADYPFTTLVPNLGVVSSGDTTFTVADVPGLIPGASE
                     GRGLGLDFLRHIERCAVLAHVIDCATLDPGRDPISDIDALEAELAAYTPALSGDSGLGD
                     LDKRPRVVILNKTDVPEAAELAEMVTPEIEARGWPVFTISAVSREGLRPLTFALAKMVR
                     DYREAHPKPEPKRQVIRPVKVKDSSFTIEKDPDIPGGFVVRGTRPERWIRQTAFDNDEA
                     VGYLADRLARLGVEDALVKKGAQPGASVTIGDVSFEWEPMTPAGIDLTRTGRGTDPRLD
                     QVERIGADERKHARRVRRGLLDEDA"
     misc_feature    51..67
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.58"
                     /tool="antismash"
     gene            1509..2612
                     /gene="proB"
                     /locus_tag="PXH69_RS19215"
                     /old_locus_tag="PXH69_19210"
     CDS             1509..2612
                     /EC_number="2.7.2.11"
                     /GO_function="GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="proB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003944552.1"
                     /locus_tag="PXH69_RS19215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_19210"
                     /product="glutamate 5-kinase"
                     /protein_id="WP_003944552.1"
                     /transl_table=11
                     /translation="MLGSTRESIASAHSIVVKIGSSALTSLVAGLDTHRLDLLADAIEA
                     RMRAGSDVVVVSSGAVGAGLAPLGLTKRPRDLATKQAAASVGQLALAHAWGTSFARYGR
                     TVGQVLLTADDIARRTQHRNAQRTLDRLRALHAVAIVNENDTVATTELRFGDNDRLAAL
                     VAHLVGADALILLSDVDGLYDGDPRKGDATLIPEVNSPEDLDGVVAGSGGALGTGGMAS
                     KLSAARLAADSGVPVLLAAASDAAQALSGADVGTAFAPRPTRLSARRFWVRHAADTSGD
                     LHLDEGAVRAVVSKRRSLLSAGIVGVSGDFHAGDVVSLMGPGEKPVARGVVAFDAAEIE
                     EILGRSTTDLPADMQRPVVHADDLVAL"
     gene            complement(2585..3442)
                     /locus_tag="PXH69_RS19220"
                     /old_locus_tag="PXH69_19215"
     CDS             complement(2585..3442)
                     /GO_function="GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007730998.1"
                     /locus_tag="PXH69_RS19220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_19215"
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="WP_042450219.1"
                     /transl_table=11
                     /translation="MPTDNSRLDALPELLAGRRLAVLTGAGISTDSGIPDYRGPDSPPR
                     NPMTFQQFIGDPAFRRHYWARNHIGWRYMDSSRPNQGHRAVAALERTGRAVGVITQNVD
                     LLHTKAGARNVIDLHGTYAQVRCLSCQRLISRTTLAERLEEANPGFAETVAAATGVEIA
                     PDADAVIESTAHFRVVDCAVCGGLLKPDIVYFGESVPKERVARAYSLVDQSDALLVAGS
                     SLTVMSGLRFVRHAAKTGKPIAIVNRGTTRGDEFADLKLEVGCAEALSALVEHESYSAT
                     KSSA"
     gene            3493..4014
                     /locus_tag="PXH69_RS19225"
                     /old_locus_tag="PXH69_19220"
     CDS             3493..4014
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019749065.1"
                     /locus_tag="PXH69_RS19225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_19220"
                     /product="VOC family protein"
                     /protein_id="WP_081305478.1"
                     /transl_table=11
                     /translation="MFNRTRVRSPILVHMNTVNPIPAGYRTITPYLAVPDGHAALDYYK
                     RALGAEIVDAMNAPDGSLMHAEIRIGDSMLQLSQEMPDFGLKSPQDGWVHSSIVVYTED
                     TDEFVARAVAEGGTLVTAVADTFSGDRHGVFLDPFGHRWAVCTKVEDVPAEEVARRARA
                     LFFPETQSSQ"
     misc_feature    complement(3501..3516)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.76"
                     /tool="antismash"
     gene            complement(4059..4991)
                     /locus_tag="PXH69_RS19230"
                     /old_locus_tag="PXH69_19225"
     CDS             complement(4059..4991)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005575166.1"
                     /locus_tag="PXH69_RS19230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_19225"
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="WP_030536631.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 5.1e-49, bitscore:
                     159.7, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MATDTSPRVAIGPVPDALLDSAVLRGGGLISPIDTADALIWAGSP
                     ETFPSELPASVRWVQLPSAGIEDWFDSGVIPRDTEITWTSAAGAFSASVAEHALMLLLA
                     GVRHLPAHIEARTWRQFEFFPKIGTLRGSTVAIIGAGGIGRALIPMLAAQGADSIAVNR
                     SGSPVPGAISTYPASRMDEIYSRADHFVIAAPATPATRHLVGRAQLAAMQPHSWVINVA
                     RGSLVDTDALVEAIESEQIGGAGLDVTDPEPLPDGHPLWDLTNAIITPHDSNPPGVRPA
                     AFADHVSKNVTRFAKGEALAALVDPVAGY"
     gene            complement(5001..5399)
                     /locus_tag="PXH69_RS19235"
                     /old_locus_tag="PXH69_19230"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009474015.1"
                     /locus_tag="PXH69_RS19235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_19230"
                     /product="ectoine synthase"
                     /protein_id="WP_003944463.1"
                     /sec_met_domain="ectoine_synt (E-value: 4.8e-54, bitscore:
                     175.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRTTTEITDTDRDITSEDGNWRSKRIVLGGDRVGFSFHETTIK
                     AGSVNEFHYANHVEAVWLVEGTGKLIDLDNNKEYDLAPGSMYLLNGHERHRVEPDTQMR
                     MLCVFNPPVTGREVHDENGVYPLVEVPA"
     gene            complement(5415..6677)
                     /gene="ectB"
                     /locus_tag="PXH69_RS19240"
                     /old_locus_tag="PXH69_19235"
     CDS             complement(5415..6677)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (27-361). E-value: 6.3e-72.
                     Score: 234.3. Matches aSDomain:
                     nrpspksdomains_PXH69_RS19240_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003944528.1"
                     /locus_tag="PXH69_RS19240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_19235"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_007731008.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.1e-74, bitscore:
                     245.2, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNINEASIFETLESEVRSYCRDWPAVFTSASGSWIRDENGRDYLD
                     FFAGAGALNYGHNNPVLKSALVDYIMSDGITHGLDMSTVAKREFLETFQRNILEPRGLD
                     YKVQFPGPTGSNTVEAALKLARKVTGRSAVINFTNAFHGMTLGALSVTGNSMKRAGAGV
                     PLVHTTPMPFDNYFDGVTEDFQWFSRVLDDSGSGINRPAAVIVETVQGEGGVNVARPEW
                     LRALATLCSDRGILLIVDDVQMGCGRTGPFFSFEEAGIVPDIVTLSKSISGYGMPMALT
                     LFKRELDVWGPGEHNGTFRGNNPAFVTSKVALDHYWSDDALTKTTLKKGARISECFANL
                     SDQFPGEVSHRGRGLVQGLVFEQPERAGKVCQLAFDEGLLAETSGPSDQVVKLLPALTI
                     TDDELEHGLSILAEATLKVCS"
     aSDomain        complement(5595..6596)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PXH69_RS19240_Aminotran_3.1"
                     /evalue="6.30E-72"
                     /label="PXH69_RS19240_Aminotran_3.1"
                     /locus_tag="PXH69_RS19240"
                     /protein_end="361"
                     /protein_start="27"
                     /score="234.3"
                     /tool="antismash"
                     /translation="TSASGSWIRDENGRDYLDFFAGAGALNYGHNNPVLKSALVDYIMS
                     DGITHGLDMSTVAKREFLETFQRNILEPRGLDYKVQFPGPTGSNTVEAALKLARKVTGR
                     SAVINFTNAFHGMTLGALSVTGNSMKRAGAGVPLVHTTPMPFDNYFDGVTEDFQWFSRV
                     LDDSGSGINRPAAVIVETVQGEGGVNVARPEWLRALATLCSDRGILLIVDDVQMGCGRT
                     GPFFSFEEAGIVPDIVTLSKSISGYGMPMALTLFKRELDVWGPGEHNGTFRGNNPAFVT
                     SKVALDHYWSDDALTKTTLKKGARISECFANLSDQFPGEVSHRGRGLVQGLVF"
     gene            complement(6726..7241)
                     /gene="ectA"
                     /locus_tag="PXH69_RS19245"
                     /old_locus_tag="PXH69_19240"
     CDS             complement(6726..7241)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003944572.1"
                     /locus_tag="PXH69_RS19245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_19240"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_003944572.1"
                     /transl_table=11
                     /translation="MKPVDTRAIPTTAPDAVLFRSPQIDDGVRLWEIARDSKVLDLNSS
                     YAYLLWCRDFSATSIVAEVDGRVVGFVSGYTRPSAQETLFVWQVAVDEDQRGKAIAARM
                     LSDLMDRTAPLGVTHLETTISPDNEASIALFTALARRREVPITRQTLFSPNDFPDGHEA
                     EDLYTIGA"
     misc_feature    7261..7275
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 18.38"
                     /tool="antismash"
     misc_feature    complement(7337..7352)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 19.26"
                     /tool="antismash"
     gene            complement(7402..9501)
                     /locus_tag="PXH69_RS19250"
                     /old_locus_tag="PXH69_19245"
     CDS             complement(7402..9501)
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1122: ATP-dependent
                     RNA helicase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248057.1"
                     /locus_tag="PXH69_RS19250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_19245"
                     /product="DEAD/DEAH box helicase"
                     /protein_id="WP_030536630.1"
                     /transl_table=11
                     /translation="MTTATAPAMRDEAERLLRELAGPDARLREDQWTAIEALVVQRKRA
                     LVVQRTGWGKSAVYFISAKLLRATGHGPTVIVSPLLALMRNQVSSAERAGVRAATINSG
                     NVTEWDDIHARVAANDLDVLLVSPERLNNPDFRDQVLPALAADAGLVVVDEAHCVSDWG
                     HDFRPDYRRIRTLIAELGEGIPVLATTATANNRVVEDVSAQLGVGGEETLVLRGGLERE
                     SLHLSVVHIPEATARAAWLADMLETLPGSGIIYTLTVSAARDLASLLSERGHTVAAYTG
                     QTDPTERESLEQDLLGNRVKALVATSALGMGFDKPDLGFVVHLGAPSSPISYYQQVGRA
                     GRSTDRADVILLPGAEDKQIWSYFASVAFPREHLVRSVIEHLDTEKATSTPALEPLVEL
                     NRTRLEMVLKVLDVDGAVKRVKGGWISTGEPWVYDAERYGRLDDARQAEQAAMVEYQSI
                     TTCRMTFLREQLDDPGLTSENSQCGRCDNCTGNRYDTTVDTAALDETRARLQRPGFDLE
                     PRKMWPTGLSKLGVKLSGKITEGPETGRILGRMSDLGWGQRLRALFDGPDAEVPDAVVS
                     ACISVLAAWNWAERPTGVMALESTTHPLLVSSLAAKLAEVGRLTDLGTLRLRPDHPPVS
                     AANSAYRVSGLVDVWEVPDMSIHQGPILLVDAIADTGWTFTMAARALRQAGAHSVLPLA
                     LASPK"
     gene            complement(9541..10362)
                     /locus_tag="PXH69_RS19255"
                     /old_locus_tag="PXH69_19250"
     CDS             complement(9541..10362)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1171:
                     transcriptional regulator, MerR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019749061.1"
                     /locus_tag="PXH69_RS19255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_19250"
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_054187684.1"
                     /transl_table=11
                     /translation="MLSIGDFARAGRVSVRMLRHYDAIGLLRPARVDASTGYRSYTAGQ
                     LALLNRIVALKELGLTLAQVQQVVESAVTVDEIRGMLTLRRAELETRIQEDTARLAHVQ
                     ARLRIIESEGQMSAYEVVIKSVPALRVAELTGSAKTMDPSSIGPVVRGLYERLCTGLDR
                     AGIAAIGPAVAYYEDAGDDENVVVHAALPVDANPDGDYEFAVVDLRAEEHVASLLHKGS
                     MDNCLPAYQALAQWIDAAGYRNAGPSREVTLACPEDVDGWVTELQEPVAKA"
ORIGIN
        1 gatccggcag tcatgtcccg attcattgac cgtgttgtgc tgcacgtcag cgctggaaaa
       61 ggcggcaatg gttgcgcctc cgtacaccgc gagaagttca aaccgctcgg cggtccggac
      121 ggtgccaacg gaggtcgcgg tggcgacgtc atcctcgtgg tcgacgagaa catccacacc
      181 ctcctcgact ttcacttcca tcccaacgcg aaggcaacca acggcaagca gggcgccggc
      241 agtaaccgcg agggtgcaaa cggtgaagac ctcatcctca aggttccgga tggaactgtg
      301 gttctcgata ccgacggcaa cgtgcttgcc gacctcgtgg gagtcggttc acgcttcgac
      361 gccgctcagg gcggccgcgg cggactcggc aacgcagcac tcgcctcgaa agcccgcaaa
      421 gctcccggtt tcgcactcct cggtgaggac ggtgtcgagc gtgacctggt tctcgaactc
      481 aagtcggtag ccgacgtcgg tctgctcgga ttcccctccg ccggtaagtc ctcgcttgtg
      541 tccgttctct ctgcggccaa gccgaagatc gccgactacc ccttcacaac tctggtcccc
      601 aacctcggcg tcgtctcgag cggtgacacc accttcaccg tcgccgacgt gcccgggctg
      661 atccccggtg caagcgaagg ccgcggtttg ggcctggact tcctgcgcca catcgaacgc
      721 tgcgccgtat tggcgcacgt gatcgactgc gccaccctcg atccgggacg tgacccgatc
      781 tccgacatcg acgccctgga agcagagctg gctgcgtaca cgcccgcgct ctccggcgac
      841 tcgggactcg gcgacctcga caagcgcccg cgcgtcgtca tcctcaacaa gaccgacgtt
      901 cccgaggccg ccgaattggc cgaaatggtc acgcctgaga tcgaagctcg cggatggccg
      961 gtcttcacca tctcggcggt gagccgagaa ggattgcgac cgttgacatt cgcgctggcg
     1021 aagatggtcc gcgactaccg cgaagctcat ccgaagcccg agccgaagcg ccaggtcatt
     1081 cgcccggtca aggtcaagga ttccagcttc accatcgaga aggatcccga tattcccggt
     1141 gggttcgtcg tccgaggcac ccgcccggag cggtggattc gtcagaccgc cttcgacaac
     1201 gacgaggccg ttggttacct cgccgaccgt cttgctcgat tgggcgtcga ggacgcattg
     1261 gtcaagaagg gcgctcagcc aggagcttcc gtcaccatcg gtgacgtcag cttcgagtgg
     1321 gagccgatga cgccggccgg tatcgatctg acccgcacgg gccgtggaac cgacccgcgt
     1381 ctcgatcagg tggagcgcat cggcgccgac gagcgcaagc acgcgcgtcg tgtccgccgc
     1441 ggcttgctcg acgaagacgc ctgacccacg cgatttcagg acgaaaaccg aagagaggga
     1501 acgggactgt gctcgggtcc accagggagt cgatagcgtc ggctcacagt atcgtcgtga
     1561 agatcggttc gtctgcgctc accagtctgg tggcgggact cgatactcac cgactcgatc
     1621 tgttggccga tgccatcgag gctcgcatgc gtgcgggttc cgatgtcgtc gttgtctcgt
     1681 ccggtgccgt cggcgccgga ctggcgccgc tgggactgac caagcgaccg cgggatctcg
     1741 cgaccaagca ggctgcggcc agcgtcggtc agttggctct ggctcacgcc tggggtacgt
     1801 cgttcgcccg ttacggccgt accgtcgggc aggttctact cacggctgac gacatcgctc
     1861 gacggacgca gcaccgcaac gcccaacgga cactcgatcg tctacgggca ttgcacgcgg
     1921 tggcgatcgt gaacgagaac gacaccgttg ccaccaccga acttcgattc ggtgacaacg
     1981 atcggctggc tgcactggtt gctcatctgg tcggcgccga cgcgttgatc ctgttgtcgg
     2041 atgtggacgg actgtacgac ggggatcccc gaaagggcga tgccacactg attcccgagg
     2101 tcaacagtcc cgaggatctc gacggtgtgg ttgccggttc gggtggcgcc ctcggtaccg
     2161 gaggcatggc gtcgaagctc tcagcagcac gcctggccgc tgattccggc gtgccggttc
     2221 tgctcgcggc agcatcggac gctgcgcagg cactcagtgg tgccgatgtc ggaacggcat
     2281 ttgcgccgcg gccgacgcga ttgtcggcac gccgattctg ggttcgtcac gccgctgaca
     2341 catcgggtga cctccacctc gacgaaggcg ccgtccgcgc ggtcgtgtcc aagcgtcgaa
     2401 gccttctgtc cgctggcatc gtcggtgtat ccggtgactt ccacgccggc gacgtcgtgt
     2461 ccctgatggg gccgggggag aagcccgtcg cccggggtgt cgttgcgttc gacgctgcgg
     2521 agatcgagga gatcctgggg cgctccacca ccgatctgcc ggccgacatg cagcgtcctg
     2581 tggttcacgc ggacgacttg gtggcgctgt agctttcgtg ctcgacgagg gcggaaagtg
     2641 cttcagcgca accgacttcg agcttgaggt cggcgaattc gtcgccgcga gtcgtgcctc
     2701 gattgacgat ggcgatcggc ttcccggtct tggcggcatg ccggacgaaa cgtaggccgg
     2761 acatcacggt caacgacgac ccggctacca acagtgcgtc ggattggtcg acaaggctgt
     2821 atgcacgggc gacgcgttcc ttggggacgg attcgccgaa gtagacgatg tccggtttga
     2881 gtaagccacc gcacacggcg caatccacga cgcgaaagtg tgcggtggat tcgatcacgg
     2941 catctgcgtc gggtgcgatt tcgactcctg ttgcagcagc gactgtttcg gcgaatccgg
     3001 gattggcttc ttccagcctc tccgcgagag tggtgcggga gatcagccgc tggcagctca
     3061 ggcatcgaac ctgcgcgtag gtgccgtgga ggtcgatgac gtttcgcgcg ccggccttgg
     3121 tgtgcaggag gtcgacgttc tgggtgatca cgccgacggc tcgccctgtt cgttccagtg
     3181 ccgcgacggc ccggtggccc tggttggggc gggagctgtc catgtatcgc cagccgatgt
     3241 ggttgcgcgc ccagtagtgc cgccgaaacg ccggatcgcc gatgaactgt tggaacgtca
     3301 tcggattgcg cgggggtgaa tcaggtccgc ggtagtccgg gattccggag tcggtggaga
     3361 tgccggcgcc cgtcagaact gccaggcgac ggccggccag gagctcgggg agggcgtcga
     3421 ggcggctgtt gtcggtaggc accgttccag gataggcgcg tgtgcgagac ggaggcgcgg
     3481 aggaagtccg aaatgttcaa tagaacacgt gtgcgatcgc caatactcgt acacatgaac
     3541 acagtgaacc cgatccctgc tggttaccgc acgatcactc cctaccttgc ggtgccggac
     3601 ggccatgccg cactcgacta ctacaaacga gcacttggag ctgagatagt cgatgcgatg
     3661 aacgcacctg acggctccct catgcatgcg gagatccgga tcggcgattc gatgctgcag
     3721 ctttcgcagg agatgccgga cttcggtctc aaatcaccgc aggacggctg ggtgcattcg
     3781 tcgatcgtcg tctacaccga ggacacggac gagtttgtcg ctcgggcagt cgccgaggga
     3841 ggcacgctgg tcacggctgt ggccgataca ttttcgggcg accggcatgg agtgtttctc
     3901 gatccgttcg gccacaggtg ggcagtatgc acgaaggtcg aggatgtacc tgccgaagag
     3961 gtcgctcgcc gggcgcgggc gcttttcttt ccggagacgc agtcgtcaca gtaatttgtg
     4021 gcaaccgtcg tccgagcatt cgagcttcgg cggcgtgctc agtatccggc tacggggtcc
     4081 acgagggcag ccagcgcctc acccttcgcg aagcgggtca cgttcttgga gacgtgatcc
     4141 gcgaaggcgg cggggcgaac gcctggcgga ttggaatcgt ggggtgtgat gatcgcattc
     4201 gtcagatccc acagtgggtg accgtccggg aggggttcgg gatcggtgac gtcgagaccg
     4261 gcgccgccga tctgttccga ctcgatggcc tcgaccagtg cgtcggtgtc gaccagtgat
     4321 ccgcgcgcga cattgatcac ccaggaatgg ggttgcatcg ccgccaactg cgcacggccc
     4381 acgaggtgac gggtggccgg ggtggcgggc gcagcaatca cgaagtgatc tgcgcgagaa
     4441 tagatttcat ccattctgga tgcggggtac gtggagatcg ccccggggac cgggctaccg
     4501 gaacgattga ctgcgatgct gtccgccccc tgcgcggcca gcatcggaat cagcgcgcgg
     4561 ccgataccgc ctgcgccgat gatcgcaacc gtcgagccac gaagggtgcc gatcttggga
     4621 aagaactcga actgacgcca cgttcgagcc tcgatgtgtg caggcaagtg gcgaactccg
     4681 gccaagagaa gcatgagtgc atgctcggcc accgacgccg aaaacgcacc ggccgccgag
     4741 gtccacgtga tttctgtgtc cctcggaatc acaccggagt cgaaccaatc ctcgattccc
     4801 gccgacggaa gctgcaccca cctgaccgac gccggtaact ccgacggaaa ggtctcgggt
     4861 gagcccgccc agatcagggc gtccgcagtg tcgatcggac tgatcagacc accgccccga
     4921 agcaccgcgc tgtcgagcag tgcatcgggg acggggccga tcgctacgcg tggagaggtg
     4981 tcagtcgcca tgactagacg ctacgcgggg acttccacca gtgggtagac accgttctcg
     5041 tcgtgcacct cacgcccggt gaccggcgga ttgaacacgc acagcatgcg catctgcgtg
     5101 tccggctcga cgcggtgacg ctcgtggccg ttgagcaggt acatcgaccc cggtgcgaga
     5161 tcgtattcct tgttgttgtc gaggtcgatc agcttgccgg tgccctcgac gagccagaca
     5221 gcctcgacgt ggttggcgta atggaactcg ttgaccgaac ccgccttgat ggtggtctcg
     5281 tggaacgaga acccgacgcg gtcgccgccg aggacgatgc gcttgctgcg ccagtttccg
     5341 tcctcactcg tgatgtcgcg gtcggtgtcg gtgatctcgg tggtggtgcg aacgatcatg
     5401 ggagagtcct tcctctagct gcagaccttg agtgtcgcct cggccagaat ggagaggccg
     5461 tgctcgagct cgtcgtcggt aatcgtcagt gcgggaagca gtttcacaac ctggtcggag
     5521 ggtccggagg tctcggcgag caatccctcg tcgaatgcca actggcagac cttgccggcg
     5581 cgctcgggct gttcgaacac gaggccctga accaagccac gcccgcggtg cgagacctcg
     5641 ccggggaact gatcggagag gttggcgaag cattcgctga tgcgtgcgcc cttcttcaac
     5701 gtcgtcttgg tgagtgcgtc gtcggaccag tagtggtcga gtgccacctt ggaggtgacg
     5761 aacgccgggt tgttgccgcg gaaggtgccg ttgtgctcgc ccggtcccca gacgtcgagt
     5821 tcacgcttga acagcgtgag cgccatcggc attccgtagc cgctgatcga cttggacaac
     5881 gtgacgatgt cgggaacgat tccggcctct tcgaaggaga agaacgggcc ggtacggccg
     5941 caacccatct ggacgtcgtc gacgatcaac aggattccgc gatccgagca cagtgttgcc
     6001 agcgcccgaa gccattcggg gcgagcgaca ttgacgccac cttcaccctg cacggtttcg
     6061 acgatcactg ccgcggggcg gttgattcca ctgccggaat catcgagaac gcgggagaac
     6121 cactggaagt cctcggtgac gccgtcgaag tagttgtcga agggcatcgg tgtcgtgtgc
     6181 acgaggggga caccggcgcc cgcgcgcttc atcgagttgc cggtcacgga gagtgcacca
     6241 agtgtcatcc cgtgaaatgc gttggtgaag ttgatgactg ccgaacgtcc ggtgaccttg
     6301 cgggccagct tcagtgcagc ctcgacggtg ttcgatccgg tcgggccggg gaactggacc
     6361 ttgtagtcca ggccgcgcgg ctcgaggatg ttgcgctgaa aggtctcgag gaactcacgc
     6421 ttggcgactg tcgacatgtc gagaccgtgc gtgatgccgt cgctcatgat gtagtcgacc
     6481 agtgcggact tgagcaccgg gttgttgtgg ccgtagttga gggcgccggc accggcgaag
     6541 aaatcgaggt agtcgcgccc gttctcgtca cgaatccagg atccggatgc gctggtgaac
     6601 actgcgggcc agtcacggca gtagctgcgt acctcggact cgagggtttc gaaaatgctt
     6661 gcttcgttga tattcatatc ttctttgttc ctccggggca tgctgtatgc cgagcgcgaa
     6721 gtgcgctagg cgccgatcgt gtaaagatct tcggcttcgt gtccatcggg aaaatcgttg
     6781 ggcgagaaca aggtctgcct ggttatcggt acttcgcgcc gacgtgcgag cgcggtgaag
     6841 agtgcgatcg aagcctcgtt gtcggggctg atcgtggtct ccagatgtgt aacacccagt
     6901 ggggcagtgc gatccatcaa gtcggacagc atgcgggcag caattgcctt gccgcgctgg
     6961 tcttcgtcga ccgcaacttg ccacacgaac agggtttcct gcgcgctggg acgggtataa
     7021 ccggaaacga atccgactac gcggccgtct acctccgcga cgatggacgt cgcgctgaaa
     7081 tccctgcacc acaacaggta tgcataactc gaattgaggt cgagcacctt cgaatcacgt
     7141 gcgatctccc agagccggac tccgtcatcg atctgtgggc ttctgaacaa caccgcgtcc
     7201 ggcgcggtgg tcgggatagc tctcgtatca acaggcttca tcggtcatct gaacgtaaca
     7261 atcattgctg tcgaagtcat gcagatgtcc gtttcgggcc gtgtggaacc cgttgcgccc
     7321 caggtgagga catgaatgtg atggtgctca cattatgtaa cttttgccgt tgtcggagcg
     7381 actatttcgg gcaaaaactc gctatttcgg gcttgcgagc gccaacggaa gtacagagtg
     7441 agcaccagcc tgtcgaagtg cgcgcgcggc catcgtgaaa gtccaaccgg tatcggcgat
     7501 tgcgtcgaca agaagtattg gtccctgatg gatactcatg tccgggactt cccaaacatc
     7561 gaccagtccg gacacgcggt aggcggagtt cgctgccgac accggagggt ggtcgggtcg
     7621 caagcgaagg gtgcccaggt cggtcaatcg cccgacctcg gccaacttgg cagccaggct
     7681 cgacaccaac aaaggatgcg tcgtggattc gagggccatg acaccggtgg gtcgctcggc
     7741 ccaattccag gcggccagaa cgctgataca cgcgctgacc accgcatcgg gtacctcggc
     7801 gtcgggaccg tcgaaaagcg cgcggagacg ttgaccccag cccaggtccg acatccgtcc
     7861 gagaatgcgc ccggtttcgg gcccctcggt gatcttcccg gacagcttga cgcccagctt
     7921 gctcaggccg gtcggccaca tcttgcgcgg ctccaggtcg aatccggggc gctgcaagcg
     7981 cgcccgagtc tcgtcgaggg cggcggtgtc gacagtggtg tcgtagcgat tgcctgtgca
     8041 gttgtcacag cgaccgcact gtgagttctc cgaggtcaga ccaggatcgt cgagttgttc
     8101 acgcaggaag gtcattcgac acgtcgtgat gctctggtac tcgaccattg cggcctgctc
     8161 ggcctggcgg gcgtcgtcga gtcggccgta gcgctcggcg tcgtacaccc agggttcgcc
     8221 ggtgctgatc cacccgccct tgacgcgttt gaccgcgccg tcgacgtcga ggactttgag
     8281 gaccatctcg agacgcgtgc gattgagctc caccagaggt tcgagtgccg gtgtcgacgt
     8341 ggccttttcg gtgtcgaggt gttcgatgac gctgcggacc aggtgctcgc gggggaaggc
     8401 gaccgaggcg aagtaactcc agatctgctt gtcctcggcg ccaggcagga ggatcacgtc
     8461 tgccctgtcg gtggaacgac cggcgcggcc cacctgctgg tagtacgaga tcggcgacga
     8521 cggtgcgccg aggtggacga cgaacccgag atcaggcttg tcgaagccca tcccgagtgc
     8581 cgaggtggcc accagagcct tgacgcgatt acccaacaag tcctgctcga gagattcacg
     8641 ttcagtggga tcggtctggc ccgtgtaggc ggcgacggta tggccgcgct cggagagcag
     8701 gcttgcgagg tcgcgcgcgg ccgaaaccgt cagcgtgtag atgatgcccg aacccggcag
     8761 cgtctcgagc atgtcggcaa gccaggcagc gcgcgccgtc gcctccggaa tgtggactac
     8821 agagaggtga agcgactcgc gttcgagccc gccgcgaagg acgagggttt cctcaccgcc
     8881 gacgccgagt tgggcggaca cgtcctcgac gacgcggttg ttggccgtcg ccgtggttgc
     8941 gaggacggga atgccctcgc cgagttcggc gatcaaggtc ctgatgcggc ggtagtcggg
     9001 acggaagtcg tggccccagt cggaaacgca gtgagcctcg tcgactacga ccaggccggc
     9061 atcagctgcc agggccggga gcacctgatc gcggaagtca gggttgttca ggcgctcggg
     9121 gctgaccaga aggacgtcga gatcattggc tgcgacgcgg gcatggatgt cgtcccattc
     9181 ggtgacgttg cccgaattga tggttgcggc gcgcactccg gcacgttcgg cagaactgac
     9241 ctggtttcgc atcagggcca acaacggaga gacgatgacg gtggggccgt ggcccgttgc
     9301 ccggagcagc ttcgccgaga tgaagtagac cgcagatttg ccccagccgg tccgctgtac
     9361 gacgagcgcg cgtttgcgct gcaccaccag cgcctcgatg gccgtccact ggtcctcgcg
     9421 taggcgggca tccggccccg cgagttcgcg gagcagccgc tcggcttcgt cgcgcatggc
     9481 tggggcagtg gcggtggtca tggaccccat ggtgcacgac cctgccgaca cgtgcgggct
     9541 tcaggccttg gcaacaggtt cctgcagttc ggtgacccag ccgtcgacat cctccgggca
     9601 cgccagcgtc acctcacggc tggggccggc attgcggtat cccgctgcgt cgatccattg
     9661 ggccaacgcc tgataagcgg gcaggcagtt gtccatcgaa cccttgtgca gcagcgaggc
     9721 cacgtgctct tcggcccgca ggtccaccac agcgaactcg tagtcgccat cgggattcgc
     9781 gtctaccggt agcgccgcat ggacgacgac gttctcgtcg tcaccggcat cctcgtagta
     9841 agcgacagcg ggtccgatcg cggcgatacc ggcacgatcc aaaccggtac aaaggcgctc
     9901 gtacaacccg cgaaccaccg ggccgatcga cgacggatcc atcgtcttgg cgctaccggt
     9961 caactccgcg acccgcagcg ccggaactga cttgatcaca acctcgtatg cggacatctg
    10021 tccctcactt tcgatgatcc gtaggcgcgc ctgtacatgc gccagtcgcg cggtgtcttc
    10081 ctggattcgg gtttcgagtt ctgctcggcg aagcgtgagc atcccgcgga tctcgtcgac
    10141 ggtgaccgct gactccacca cttgctggac ctgggccaga gtcaggccca actccttgag
    10201 agcgacgatg cggttgagca gcgccaactg gcccgcggtg tacgagcgat acccggtgga
    10261 tgcgtcgacc ctcgcgggtc tgagcagacc gatggcgtcg tagtggcgga gcatgcgcac
    10321 cgacacacga cctgctctcg cgaagtctcc gatgctcaac atccttgctc gccccgtttc
    10381 tctctcacaa acccgatac
//
