LOCUS       NZ_JARDXE010000008     22816 bp    DNA     linear   CON 01-APR-2024
DEFINITION  Rhodococcus qingshengii strain HQ HQ1_scaffold8, whole genome
            shotgun sequence.
ACCESSION   NZ_JARDXE010000008
VERSION     NZ_JARDXE010000008.1
KEYWORDS    WGS; RefSeq.
SOURCE      Rhodococcus qingshengii
  ORGANISM  Rhodococcus qingshengii
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Nocardiaceae; Rhodococcus; Rhodococcus erythropolis group.
REFERENCE   1  (bases 1 to 310594)
  AUTHORS   Hu,J. and Xu,J.
  TITLE     A novel hydrolase synthesized by Rhodococcus erythropolis HQ is
            responsible for the detoxification of Zearalenone
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 310594)
  AUTHORS   Hu,J. and Xu,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-FEB-2023) Grain and Oil Risk Assessment Laboratory,
            Jiangsu Academy of Agricultural Sciences, No. 50, Zhongling Street,
            nanjing 210014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2022
            Assembly Method                   :: ABySS v. 2.1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: GCA_002079265.1
            Genome Coverage                   :: 470.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029076895.1-RS_2024_04_01
            Annotation Date                   :: 04/01/2024 06:35:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,927
            CDSs (total)                      :: 6,855
            Genes (coding)                    :: 6,768
            CDSs (with protein)               :: 6,768
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 33 of 87
            Pseudo Genes (incomplete)         :: 63 of 87
            Pseudo Genes (internal stop)      :: 12 of 87
            Pseudo Genes (multiple problems)  :: 18 of 87
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:34:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 49871
            Orig. end                         :: 72687
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JARDXE010000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..22816
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="redox-cofactor"
                     /region_number="2"
                     /rules="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /tool="antismash"
     cand_cluster    1..22816
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cds(PF04055 and (TIGR04085 or SPASM))
                     and (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /kind="single"
                     /product="redox-cofactor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22816
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12816](-)"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..12816)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
     misc_feature    149..164
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 18.06"
                     /tool="antismash"
     gene            complement(225..1706)
                     /gene="mftG"
                     /locus_tag="PXH69_RS13695"
                     /old_locus_tag="PXH69_13690"
     CDS             complement(225..1706)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /gene="mftG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007730154.1"
                     /locus_tag="PXH69_RS13695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_13690"
                     /product="mycofactocin system GMC family oxidoreductase
                     MftG"
                     /protein_id="WP_161120433.1"
                     /transl_table=11
                     /translation="MTEADYADFLVVGGGTCGCVVAARLSEDPSAMVMLLESGSGYRSA
                     LELPDVLGDPYRLPVGPASEYTWTYPVELTPRRASTIARGRTLGGSGAVNGAYFTRATR
                     ADFENWPSSWRYDDVLPYFKKSETDHDFESEFHGTAGPIPVERRAWDQLHPLSGEFHAA
                     ALGAGFPDDVDKNAPDSFGVGRVPLNVSDHRRISTAIGYLMPALHRPNLRVESGVNVIR
                     IAFSGTRAIGIDVLDDGNVRRIRADHVVVCSGAVATPHILLNSGVGPAEQLVGQGVSVV
                     LDRPGVGQNFVDHPEVLLPYHFSKPRTIRSQTPVLETALNLAEIEIRPYTASFTDLVPG
                     APRMDHGVGVVLMAPRSRGSIELVSGDPAGAPRIRYNYVASAHDRAAIREGMQIAENLL
                     ESIAETGLIDRPVVEYTDEWVESRLGTSLHMSGSCVMGAEADPFAVVDGRCRVIGAQGL
                     SIVDTSILPTIPTRGPHATAVMVAERASAILLGDE"
     gene            complement(1703..3100)
                     /gene="mftF"
                     /locus_tag="PXH69_RS13700"
                     /old_locus_tag="PXH69_13695"
     CDS             complement(1703..3100)
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003942091.1"
                     /locus_tag="PXH69_RS13700"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_13695"
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="WP_007730153.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 1.2e-24,
                     bitscore: 81.3, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRPARLPDGFGIRLDPKVRTYSGGRVLIGGSPTRMLKLAPTAAAM
                     IGDGFLEVVDQQSAAVARHLLDSGVANPRPMSTPSAADVTVVIPVKDNQAGVERLLPVL
                     KDLTVIVVDDGSEVPVEPRRACPGTGTITVVRHESARGPSAARNSGLRSAQTRFVAFLD
                     SDVIPRAGWLELMLGHFSDPGVALVAPRIVALDPYGTALARYENMRSSLDLGRKEAAVK
                     SGSPVAYVPSAAVIVRRDVALECNGFDESLEVAEDVDFCWRLQAAGWRLRYEPVAHVAH
                     DHRVQFDKWFARRAFYGTGAAPLAARHEGSVPPMAMSFSTLFACIAAATLTRSGLAGAL
                     GALLFTVYRLRKMFTGLSQPTRIAAILTAQGVVGGFWQLASAMCRHYWPVTLLAVIASR
                     RIRRLAVAAAVTEGLVDWYRHREPGGLGPVRYVFFKRADDIAYGAGLWRGAFDARDWAA
                     LKPRITK"
     gene            complement(3198..3890)
                     /gene="mftE"
                     /locus_tag="PXH69_RS13705"
                     /old_locus_tag="PXH69_13700"
     CDS             complement(3198..3890)
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019744248.1"
                     /locus_tag="PXH69_RS13705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_13700"
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="WP_054186896.1"
                     /transl_table=11
                     /translation="MSSPRDLAAIPSPALEGTALTVVVPVGSFEQHGPHLPLDTDTRIA
                     RAVAQALPDVVVAPAIEYGASGEHEGFAGTISIGAEAMETVVVEYGRSAFRWTKRVLFV
                     NGHGGNGPALAKAVALLRYEGRDAAWAPCAVPGADAHAGHTETSLLLHLSPDLVHMSLA
                     ETGNLSPIADLMPGMRSGGMVAVSQNGVLGNPVGATAEDGAYIFGELVGRVTDGLARWE
                     PGPDGRLR"
     misc_feature    complement(3931..3944)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.33"
                     /tool="antismash"
     gene            complement(3991..5228)
                     /locus_tag="PXH69_RS13710"
                     /old_locus_tag="PXH69_13705"
                     /pseudo=""
     CDS             complement(3991..5228)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019745836.1"
                     /locus_tag="PXH69_RS13710"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_13705"
                     /product="DUF222 domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MDSREAWQLDGVGLKSEVLDLVRARHELQSRMVLLIVEMFSREVL
                     GGKGFRRSRSGCTGRRIWRSVSAACSSGWRGC"
     gene            complement(5270..7219)
                     /locus_tag="PXH69_RS13715"
                     /old_locus_tag="PXH69_13710"
     CDS             complement(5270..7219)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019744250.1"
                     /locus_tag="PXH69_RS13715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_13710"
                     /product="mycofactocin system FadH/OYE family
                     oxidoreductase 2"
                     /protein_id="WP_275231837.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 4.4e-13, bitscore:
                     43.3, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPYPRLFAPLRLGNLTLRNRVVFSAHLTNYAENGLPTEQHASYY
                     EARARGGAGLIITEEHSTHPWDRPYEKMIAGYRPEAIEGYRRITDAVHAHGSSILAQIN
                     HNGGQGAGTYSRSPLLAPSAVADPLFREVPKAVDDADIAEILAGFALVARNCVAGGFDG
                     VELQASQSSIVRAFLAPSTNVRTDRYGGGVAHRARFLLEVLGVLRKAIGPDRVLGVRLS
                     GYEGTRGGIELSDAVATAQLIEADGYVDYINTTVGMATETLHLVEASMAVPTGYANFVP
                     SAIREVVDLPVVGVGRFKTPEQAEAALEDGVCDLVGVVRGQIADPDFVNKARAGQSVQV
                     RTCLSCNQECVGRMGLGRWLGCTENPRVGRESVPLPMPRRRGRTVVVVGGGPAGLQAAA
                     TAAQLGHAVTLFERERRLGGQILTASVVPARRELADLVRNLESDCRRSGVTIELGAGAD
                     LAIVEERAPEVVVIATGARPLRPAWAGGSERVVGVRDVLDGKILPSGTVLVYDELGFHQ
                     GTSVAEFLADRGCAVTIATNGMVVAQDLSITLDFEGWNRRAFEKSIVQRTDLMVTSVSA
                     GASPLVSLVHLPTGVTEEVAVDWVVFATQQEPVDELWNTLQGSCFEKFRIGDALSPRRS
                     HAAVIEGHRAALAI"
     gene            complement(7219..8046)
                     /locus_tag="PXH69_RS13720"
                     /old_locus_tag="PXH69_13715"
     CDS             complement(7219..8046)
                     /NRPS_PKS="Domain: PKS_KR (12-187). E-value: 3.5e-15.
                     Score: 48.3. Matches aSDomain:
                     nrpspksdomains_PXH69_RS13720_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003941966.1"
                     /locus_tag="PXH69_RS13720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_13715"
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="WP_003941966.1"
                     /sec_met_domain="adh_short (E-value: 4.3e-32, bitscore:
                     105.8, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQGSVRSLEGRVAFITGAARGQGRAHAVKMAREGAAIIAVDVCA
                     SVASDNSYDAATSEDFAETIRLVEAEGGKILAREVDVRDGAALTAVVKDGVEQFGRLDI
                     VVANAGVCNWNRFWEMSDEQWETLIDINLTGVWKTLKASVPAIIEGGRGGSIIVVSSVA
                     GLKALPGQAHYASAKFGLVGLTQAAAKELGEYKIRVNSIHPYGVNTPMGVDQGALEVFA
                     KFPQYLPNFTPILSDIAFAEPDEIADTVLWLAGDGSRTVTASHIALDQGNSKV"
     aSDomain        complement(7486..8010)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PXH69_RS13720_PKS_KR.1"
                     /evalue="3.50E-15"
                     /label="PXH69_RS13720_PKS_KR.1"
                     /locus_tag="PXH69_RS13720"
                     /protein_end="187"
                     /protein_start="12"
                     /score="48.3"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VAFITGAARGQGRAHAVKMAREGAAIIAVDVCASVASDNSYDAAT
                     SEDFAETIRLVEAEGGKILAREVDVRDGAALTAVVKDGVEQFGRLDIVVANAGVCNWNR
                     FWEMSDEQWETLIDINLTGVWKTLKASVPAIIEGGRGGSIIVVSSVAGLKALPGQAHYA
                     SAKFGLVGLTQA"
     CDS_motif       complement(7516..7578)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PXH69_RS13720_0001"
                     /evalue="1.40E-04"
                     /label="PKSI-KR_m4"
                     /locus_tag="PXH69_RS13720"
                     /protein_end="177"
                     /protein_start="156"
                     /score="14.4"
                     /tool="antismash"
                     /translation="IVVSSVAGLKALPGQAHYASA"
     gene            complement(8043..9974)
                     /locus_tag="PXH69_RS13725"
                     /old_locus_tag="PXH69_13720"
     CDS             complement(8043..9974)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728304.1"
                     /locus_tag="PXH69_RS13725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_13720"
                     /product="mycofactocin system FadH/OYE family
                     oxidoreductase 1"
                     /protein_id="WP_275231838.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.6e-13, bitscore:
                     44.7, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLDEHTALAGRLVRSRVVFGPHETNLAVGRSLSDRHRAYYQRRA
                     EGGTGIVVTEVASVSENDWPYERAPLASQCGPGWQSIAAACAPYGTLVLAGLGHTGMQG
                     SSAYSQSVLWGPSRVADPATRELPMAMGQPEIDQLIVGFREASALAVASGMDGVELDAG
                     PRSILRQFLSGLTNTRQDRYGSDRVALVREVISAVREEVGSDHVVSLRLSCDEEAPWAG
                     LTPDLAIGIAREVSPFLDLLVVVRGGLYSSDRYRPDFHCPPNFNLDLCREIKVALSIPV
                     VVQGSVVDIADAHAALEVGEFVEMTRAQIADPDLVVKASRGDRPRPCVLCNQTCLVRDP
                     RNPVVTCVGNPSAGFETVDPDEREQISTPGAVLVVGGGPAGMEAARISAVAGHSVTLVE
                     SLSQFGGMIRTFARGDGRSRFALLAQWLEDECRRSGVTMRTGAALDAQDLGDARSRGEF
                     VVVATGSAARPPTYPVDDSVRWLDASEAFEKLSTVGPGPVALIDPLGGPIAVALAEALA
                     AHGISVSIVTPDSIVGSRLGASGDLVGANTRIQQAGITRVLASRVTEIARGAVCVEHVH
                     TGERSQLECEVVIDCSPRLPNETLKEEADIQVGDSVAPRTVLESIREGRFAGFTTRTSF
                     NSAERAIS"
     gene            complement(10001..11179)
                     /gene="mftD"
                     /locus_tag="PXH69_RS13730"
                     /old_locus_tag="PXH69_13725"
     CDS             complement(10001..11179)
                     /EC_number="1.4.3.26"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR03966"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003942047.1"
                     /locus_tag="PXH69_RS13730"
                     /note="MftD, an enzyme found in the mycofactocin
                     biosynthesis locus, performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="PXH69_13725"
                     /product="pre-mycofactocin synthase MftD"
                     /protein_id="WP_003942047.1"
                     /sec_met_domain="TIGR03966 (E-value: 3.5e-221, bitscore:
                     727.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKNAFFETVAEAQRRAKKRLPKSVYAALVAGSEKGLTVDDNVAA
                     FSELGFAPHAAGLSDKREMSTTIMGQDISLPVMISPTGVQAVHPDGEVAVARAAAARGT
                     AIGLSSFASKSIEEVAAANPQVFFQMYWVGSRDVLLQRMERARAAGAKGLIITTDWSFS
                     YGRDWGSPSIPEKMDLKAMFQFAPEGIMRPKWLLEFAKTGKIPDLTTPNLAAPGQPAPT
                     FFGAYGEWMQTPLPTWEDIAWLREQWGGPFMLKGIMRIDDAKRAVDAGVSAISVSNHGG
                     NNLDGTPAPIRVLPGIAEAVGDQVEVVLDGGIRRGGDVVKALALGAKAVMLGRAYLWGL
                     SANGQAGVENVLDLMRMGIDSGLMGLGHSSITELSPADLVIPEGFTRTLGAS"
     gene            complement(11235..12452)
                     /gene="mftC"
                     /locus_tag="PXH69_RS13735"
                     /old_locus_tag="PXH69_13730"
     CDS             complement(11235..12452)
                     /EC_number="1.3.98.7"
                     /EC_number="4.1.99.26"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: SPASM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR04085"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930977.1"
                     /locus_tag="PXH69_RS13735"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron carrier
                     mycofactocin from the terminal Val-Tyr dipeptide of the
                     precursor peptide MftA.; MftC is a radical SAM/SPASM enzyme
                     that catalyzes the first two steps in biosynthesis of the
                     electron carrier mycofactocin from the terminal Val-Tyr
                     dipeptide of the precursor peptide MftA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="PXH69_13730"
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="WP_003942089.1"
                     /sec_met_domain="PF04055 (E-value: 3.1e-24, bitscore: 80.4,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_12 (E-value: 1.4e-07, bitscore: 26.1,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 4e-11, bitscore: 37.0,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 8e-16, bitscore: 52.5,
                     seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 5e-07, bitscore: 24.4,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSVLEQPSPPAKVGRLVDQFELGLDAPICLTWELTYACNLSCVH
                     CLSSSGRRDPNELSTEQCKSIIDELQRMQVFYVNIGGGEPTVRSDFWELVDYATDHQVG
                     VKFSTNGVKIDKKVAARLAASDYVDVQISIDGATAEVNDAVRGPGSFAMAERALANLKE
                     AGFKDAKISVVVTRHNVSQLDEFKALADKYDATLRITRLRPSGRGADVWDDLHPRPEQQ
                     RELYNWLVANGEGVLTGDSFFHLSAFGDALPGLNLCGAGRVVCLIDPVGDVYACPFAIH
                     EQFLAGNIVKDGGFQEVWQHSDLFEELRSPQTGGACSKCNHYDSCRGGCMAAKFFTGLP
                     MDGPDPECVIGNGEMALASAGEVPKSSVDHSRSGQRKTPRASVPLTLMMRPPAKICDEN
                     PLAGMQ"
     gene            complement(12529..12816)
                     /gene="mftB"
                     /locus_tag="PXH69_RS13740"
                     /old_locus_tag="PXH69_13735"
     CDS             complement(12529..12816)
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: Mycofactocin_RRE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728309.1"
                     /locus_tag="PXH69_RS13740"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ, a
                     cofactor that derives entirely from a Tyr and a Glu in the
                     precursor PqqA.; MftB, a small protein, is a peptide
                     chaperone that assists the radical SAM enzyme MftC in
                     performing two modifications to the C-terminal Val-Tyr
                     dipeptide of the mycofactocin precursor peptide, MftA.
                     MftB's role is analogous to the role of PqqD in the
                     biosynthesis of PQQ, a cofactor that derives entirely from
                     a Tyr and a Glu in the precursor PqqA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="PXH69_13735"
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="WP_007728309.1"
                     /sec_met_domain="Mycofactocin_RRE (E-value: 1.7e-31,
                     bitscore: 102.5, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRVLTVSALDLDAGWKLHPQVALRPEPFGALLYHFGTRKLSFLK
                     NTTIVAVVKALPDHPDVRSALRAQGIEDDAAPQYARALSTLADSHMIVPV"
     aSDomain        complement(12541..12780)
                     /aSDomain="Mycofactocin_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a mycofactocin
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_PXH69_RS13740_Mycofactocin_RRE.1"
                     /evalue="1.60E-32"
                     /identifier="RREFam016.1"
                     /label="PXH69_RS13740"
                     /locus_tag="PXH69_RS13740"
                     /protein_end="92"
                     /protein_start="12"
                     /score="102.5"
                     /tool="antismash"
                     /translation="DAGWKLHPQVALRPEPFGALLYHFGTRKLSFLKNTTIVAVVKALP
                     DHPDVRSALRAQGIEDDAAPQYARALSTLADSHMI"
     gene            complement(12801..12896)
                     /gene="mftA"
                     /locus_tag="PXH69_RS13745"
                     /old_locus_tag="PXH69_13740"
     CDS             complement(12801..12896)
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005573048.1"
                     /locus_tag="PXH69_RS13745"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes in
                     Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /old_locus_tag="PXH69_13740"
                     /product="mycofactocin precursor MftA"
                     /protein_id="WP_063836238.1"
                     /transl_table=11
                     /translation="MSDRDNKALENDLVEESLVEEVSIDGMCGVY"
     misc_feature    12880..12896
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 18.73"
                     /tool="antismash"
     misc_feature    complement(12973..12988)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.11"
                     /tool="antismash"
     misc_feature    complement(12983..12998)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.13"
                     /tool="antismash"
     gene            13047..13646
                     /gene="mftR"
                     /locus_tag="PXH69_RS13750"
                     /old_locus_tag="PXH69_13745"
     CDS             13047..13646
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0036042 - long-chain fatty acyl-CoA
                     binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene="mftR"
                     /gene_functions="regulatory (smcogs) SMCOG1215: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728311.1"
                     /locus_tag="PXH69_RS13750"
                     /note="MftR, the mycofactocin system transcriptional
                     regulator, is an uncharacterized TetR family DNA-binding
                     transcription factor. Its role is inferred by context. It
                     occurs as part of the biosynthesis locus for mycofactocin,
                     a partially characterized electron carrier derived from the
                     terminal Val-Tyr dipeptide of the precursor peptide MftA,
                     through a radical SAM enzyme-mediated process; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="PXH69_13745"
                     /product="mycofactocin system transcriptional regulator"
                     /protein_id="WP_007728311.1"
                     /transl_table=11
                     /translation="MRSRKKPSARIGRRPSTTKEGISTIGIELFSTVGFNEVSVDQIAD
                     AAGIARRTFFRYFPSKNAVPWGDFDGHLDEMREHLDSLPADIPLVDALESALLTFNTFP
                     PEETAVHRKRMELILEVPTLQAYSVVMYEGWRQVVADYVARRMNLEPTDHGPRTVGYLL
                     LGVAMSAYEQWLTDDSLELPELLKTGTQSLRTGIST"
     gene            13676..14509
                     /gene="mftM"
                     /locus_tag="PXH69_RS13755"
                     /old_locus_tag="PXH69_13750"
     CDS             13676..14509
                     /codon_start=1
                     /gene="mftM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728312.1"
                     /locus_tag="PXH69_RS13755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_13750"
                     /product="mycofactocin oligosaccharide methyltransferase
                     MftM"
                     /protein_id="WP_007728312.1"
                     /transl_table=11
                     /translation="MSTITNEPVLDSLAPSTPGLWSFGHVTVERVESGPISLDRRGGRL
                     HVRHSLTPADLSERLVAGVTSSIQDAEFGQDEFELTMVGLVRSSVDGALESWTTYYRNS
                     LAELLDGTADFAPIHDKAEELVRGSVLDLGSCFGFLPLRLARAGMDVTATDILPGTMIL
                     LDAVAPELGITIDTVICDAGEVPAPDNSADTVTAIHLLEHVDDAVGEAVIAQALRIARQ
                     RVVIAVPFEDEATACHGHIRTFDLESLRAVGRQSRVPFEVFEHHGGWLVLDASAA"
     gene            complement(14516..14719)
                     /locus_tag="PXH69_RS13760"
                     /old_locus_tag="PXH69_13755"
     CDS             complement(14516..14719)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003942010.1"
                     /locus_tag="PXH69_RS13760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_13755"
                     /product="DUF4287 domain-containing protein"
                     /protein_id="WP_007728313.1"
                     /transl_table=11
                     /translation="MTEPVKGPASYFPSIEKKYGRPIGEWQELIRSSDLTKHMELVNWL
                     KSEHGMGHGHANALVAHTLKEG"
     gene            14861..15073
                     /locus_tag="PXH69_RS13765"
                     /old_locus_tag="PXH69_13760"
     CDS             14861..15073
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005058188.1"
                     /locus_tag="PXH69_RS13765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_13760"
                     /product="TOBE domain-containing protein"
                     /protein_id="WP_003942061.1"
                     /transl_table=11
                     /translation="MRLSTRNQLTGTITEVNLGTVMATVKVKLDGGNQIVTSSITRDAA
                     LDLDLEPGQAATVFVKSTEVTIGVE"
     gene            complement(15090..15515)
                     /locus_tag="PXH69_RS13770"
                     /old_locus_tag="PXH69_13765"
     CDS             complement(15090..15515)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019744256.1"
                     /locus_tag="PXH69_RS13770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_13765"
                     /product="DUF6314 family protein"
                     /protein_id="WP_007728314.1"
                     /transl_table=11
                     /translation="MVDDAKIEPRALVGKWHFERDIVDRLSGSTKHVFGETTISDAGDG
                     RMRWYESGTLRDGDLELPVFRTLFVAERGLEWAVTFEDGREFHDWAPGEEVEHLCGADT
                     YRGRIDVTAGDRSTWSVVWTVTGPSKDYTMTTHLTRP"
     gene            15626..16102
                     /locus_tag="PXH69_RS13775"
                     /old_locus_tag="PXH69_13770"
     CDS             15626..16102
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728315.1"
                     /locus_tag="PXH69_RS13775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_13770"
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="WP_007728315.1"
                     /transl_table=11
                     /translation="MNDSAALHKRAVFAEDLEIGQVIPLGSYSVTEEEIVEFATQWDPQ
                     WFHVDKEAAESGVFGGLIASGLHTLSICQKLVVASLYDQWNVIAGKTMRDVSFLRPLRP
                     GDTLSGKVTVDNVVFDNRARGLVTITAKLVDAHGNRVLDVVTDAYLHCRSTRGQ"
     gene            16336..17124
                     /locus_tag="PXH69_RS13780"
                     /old_locus_tag="PXH69_13775"
     CDS             16336..17124
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003941978.1"
                     /locus_tag="PXH69_RS13780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_13775"
                     /product="thioesterase family protein"
                     /protein_id="WP_065352149.1"
                     /transl_table=11
                     /translation="MAITSQVSVAAAPSTDAARHHTEFDSSWRSFDGIQGGLAIARMLR
                     VAADASGAEPLAVTAHLDRPVPPGPAELTVGEMRGGRTVSCHVTLTGSGSALVRLARDI
                     GATTMSAPVAEPAPADPSTLPPLEIPVDFVPFAQHLDIRPINSARPFAGGDSPEFDVWI
                     RLLTDLDFTAQERAAVLLDALPPGLFATLTMPVAIPTIEFTAHFTPEHLTADPRTLSPW
                     YRLRHGTAWATDTLCVDESELFDSTGRLAARARQLRRILS"
     gene            complement(17137..18807)
                     /locus_tag="PXH69_RS13785"
                     /old_locus_tag="PXH69_13780"
     CDS             complement(17137..18807)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003941963.1"
                     /locus_tag="PXH69_RS13785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_13780"
                     /product="hypothetical protein"
                     /protein_id="WP_007728318.1"
                     /transl_table=11
                     /translation="MTVVVEAAPPVPASASQFTGTRRLVRLALRRDRIQLSVWVISIVL
                     FMLLVVASVTGLYSTPEELRNIAVSSAVSPVALATNGIVSGDSQGAIVASQSVLVFALV
                     AALMSTLAVVRHTRQNEETGRAEMIGAAVVGRRALLTSALIVTVGANVVLALGIALGSI
                     AVGLPVVGSFALGASIGGTGIAFAGVAAVTAQITDGARTANGLAGAAIGVAFMLRAIGD
                     VNSTVVDDGTRVVSGWLSWLSPIGWAQQIRPFDDDAWWILLLLLGFAAVHVVVAFVLIG
                     HRDQGAGLVQPRPGPPVAASWLPSAWGLAWRMQRMTMFWWIFAVVILGFAYGAVGNEID
                     AMVGSSQQTAEMFEKLGGGGSDLVDSYLATALGLTSIVIAAYSVQSLLRMRGEETSGRL
                     EPLLATSLSRWRWMLSHLGVVVGGVILAHVLMGAFTGFAFGLVTDDIGGNTLRMTGAAL
                     AYLPAVLVVVGLVSLAFGLFPTYASALSWGILAACIVLGQLGTLLKLPQVVLDLSPFTH
                     VPGAPAEPVTVLPLLAMIVVAVVLSTASLVAFRARDLQV"
     gene            complement(18804..19739)
                     /locus_tag="PXH69_RS13790"
                     /old_locus_tag="PXH69_13785"
     CDS             complement(18804..19739)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019744261.1"
                     /locus_tag="PXH69_RS13790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_13785"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_007728320.1"
                     /transl_table=11
                     /translation="MGAAISVKSLVKTFGTTRALDGLDLDVSTGEVHGFLGPNGSGKST
                     TIRVLLGLLRSDSGDATVLGGDPWRDAVTLHRRLAYVPGDVNLWPNLTGGEAIDLLAGL
                     RGGLDRKRRSELLDRFELDPTKKARTYSKGNRQKVALVAAFSSDVDLYVLDEPTSGLDP
                     LMEAVFQDCVEEVAHAGKTVLLSSHILAEVEALCDRVSIIRDGRTVEEGTLSELRHLTR
                     TSVAAETQLPIGGLDGLGGVHDAVIDGNHVTCEVDSAQLGPLMTYLVGFGIVALTSTPP
                     TLEELFLRHYGDERARETSVHLRAETGVRP"
     gene            19808..20302
                     /locus_tag="PXH69_RS13795"
                     /old_locus_tag="PXH69_13790"
     CDS             19808..20302
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728322.1"
                     /locus_tag="PXH69_RS13795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_13790"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_007728322.1"
                     /transl_table=11
                     /translation="MTDTSHTVAPDESRHYVERLSLVLSDMGFPPMPARVWAALMTSET
                     PSSTPGDLGSRLGVSPAAISGAVRYLQQVGLIERVAVPGSRRQHYCVSVDMWADAFVTK
                     QSSLYQVSAVADEGIRVLGEGTTAGDRMADLRDFFDFMAAEMPRMLADWRSRRKDRTPG
                     R"
     misc_feature    19854..19872
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.62"
                     /tool="antismash"
     gene            20386..20925
                     /locus_tag="PXH69_RS13800"
                     /old_locus_tag="PXH69_13795"
     CDS             20386..20925
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003941864.1"
                     /locus_tag="PXH69_RS13800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_13795"
                     /product="hypothetical protein"
                     /protein_id="WP_007728324.1"
                     /transl_table=11
                     /translation="MKLSIRGANTSPTPPFARTASPETCERSISLNRTAAEAFAGDHHA
                     LSKLLARLHPRVVLYYRARLLGTPVATFADDLAHQCTLDVVTRMSDTISADTRIETFAY
                     ELASRRIASIPQSRNIDGQFKRPRRLTDDQREVLVLRIAVGLTAEMTASTLRTTRSSVL
                     LLQHQALRAWTVSEQS"
     gene            complement(20922..21605)
                     /locus_tag="PXH69_RS13805"
                     /old_locus_tag="PXH69_13800"
     CDS             complement(20922..21605)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019744264.1"
                     /locus_tag="PXH69_RS13805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PXH69_13800"
                     /product="response regulator transcription factor"
                     /protein_id="WP_003942005.1"
                     /transl_table=11
                     /translation="MTTARPVITRGASPVQGLVRIVLVDDHAILRQGLRSVLERESDLE
                     IVGEASSLAEALAVVDKVGPDIVLMDLKLSASSDYEGLTLCGQLSSAHPGLGLLVLTTF
                     LDDQLVVRAVHAGARGYVVKDVDTTELVRAIRAVSRGESAFDSRSASAVVRSLNGQSSS
                     KERLTDRELEVLRLLANGLSNIKIGERLFISPTTVKFHVSNIMRKLEVSRRAEAVYAAS
                     KQGLI"
     misc_feature    21635..21646
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 17.0"
                     /tool="antismash"
     misc_feature    complement(21803..21821)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 22.94"
                     /tool="antismash"
ORIGIN
        1 ccgcgggcat cgatgaacga ctgatgggca gacgaaagtc gccgcgcgag taacgcgacg
       61 tcatcgcctg gcctgaccgc cacccacggg ttgccgggca cagcctgttc acgtgccata
      121 ttttgcgttc ctccgctccg tggaccactg taacgccgat cacagcccat aggcgagaac
      181 gaccattcgg tatgactgcc gacccagcct gctgcccctc gcggtcactc gtcaccgagc
      241 agaatggcgc ttgctcgttc cgcgaccatc accgcggtcg catgcgggcc gcgggtcggg
      301 atcgtcggca ggattgacgt gtcaacaata ctgagtcctt gcgcgccgat cacacggcac
      361 cgaccgtcga cgactgcgaa cgggtccgcc tcggcaccca tcacgcaact accggacata
      421 tgcagcgacg ttcccaggcg ggattcgacc cactcatcgg tgtactccac gacggggcga
      481 tcgatcaacc ccgtctcggc gatcgactcg agaagattct cggcgatctg catcccttcc
      541 cgaatggcag ctcgatcgtg cgccgacgcg acatagttgt accgaatacg cggcgcccca
      601 gcgggatccc ccgataccag ttcgatgctt ccccggctgc gcggcgccat caggaccact
      661 ccgacgccgt ggtccatgcg aggcgcaccg ggaaccaggt ctgtgaaaga tgcggtgtac
      721 ggacggattt cgatctcagc gaggttgagg gctgtctcca gcaccggagt ctgcgatcga
      781 atcgtgcggg gcttcgaaaa atggtacggc agcagcactt ccgggtggtc gacgaagttc
      841 tgtccgacgc ccggtcgatc gagaacgacg gaaactccct gccctacaag ttgttccgct
      901 ggaccaacac cggaattgag gagaatgtgc ggtgtcgcca ccgcgcccga acacacgacc
      961 acatggtcgg cacgaatgcg tcggacattg ccgtcgtcga gaacgtcgat gccgatcgca
     1021 cgggtacccg agaacgcgat acgaatgacg ttcactccgg attccacccg cagattcggc
     1081 cggtgcaacg ccggcatcag atacccgatc gccgtactga tccgacgatg atccgacacg
     1141 ttcagtggca ctcggcccac cccgaacgag tccggcgcat tcttgtcgac gtcgtcggga
     1201 aagcctgctc cgagcgctgc cgcatgaaac tcgccgctca gtggatgcag ttggtcccac
     1261 gctcgacgct cgaccgggat cggtccggcg gtcccgtgga actcgctctc gaaatcatga
     1321 tccgtttcgg acttcttgaa gtacggcaag acgtcgtcgt atcgccatga actcggccaa
     1381 ttctcgaaat cagcacgggt cgcgcgggtg aagtacgcac cgttgacggc tccggatcca
     1441 ccgagtgtcc gcccccgcgc aattgtggac gcccgacgcg gcgtcagttc gaccggatag
     1501 gtccacgtgt attcgctcgc aggtccgacc ggcagtcggt acggatcacc caggacgtcc
     1561 ggaagttcga gcgccgatcg atatccgctt cccgattcga ggagcatcac catggcggat
     1621 ggatcttcac tgagcctggc tgcgaccacg caaccacacg tgccaccacc gacaaccagg
     1681 aaatctgcgt agtcggcttc tgtcacttgg tgattcgagg tttcagcgcc gcccagtcgc
     1741 gggcgtcgaa tgcgccacgc cagagtccgg ctccatacgc gatgtcgtcg gctcgtttga
     1801 agaagacgta ccgaaccggt cccagcccgc ctggctcacg gtggcgatac cagtccacca
     1861 aaccttcggt gactgctgca gcgacggcca gccgccgaat tcgacgggag gcgatcaccg
     1921 ccaggagcgt cacgggccag tagtgacgac acattgccga ggcgagttgc cagaatccgc
     1981 cgacaactcc ctgcgcggtg aggatcgccg cgatccgggt gggttgactc aggccggtga
     2041 acatctttcg cagccggtaa acggtaaaca gcaaagcgcc gagtgcgccg gcaagaccgg
     2101 aacgggtcag tgttgcagcg gcgatacacg cgaagagcgt cgagaacgac atcgccatcg
     2161 gcggaaccga accctcgtgt cttgcagcca acggcgccgc acccgtgccg tagaacgctc
     2221 tcctggcgaa ccacttgtcg aactgaactc gatgatcgtg ggcgacatgc gcaaccggtt
     2281 cgtacctcag tcgccaaccc gcagcctgca gtcgccagca gaaatcgacg tcctcggcta
     2341 cttcgagact ctcgtcgaaa ccgttgcatt cgagtgccac gtcgcgccgg acgatcacgg
     2401 cggcgctggg gacataggcg accggactgc ccgacttcac cgcagcttcc ttgcgtccga
     2461 ggtcgagaga cgatcgcatg ttctcgtacc gcgccagcgc cgtcccgtac ggatcgaggg
     2521 cgacgatgcg gggtgcaaca agggcaaccc ccggatcgct gaagtggccc aacatcaact
     2581 cgagccaacc ggcacgcggt atgacgtcgg agtccagaaa agctacgaaa cgtgtttggg
     2641 ccgaacgcaa tcccgagttt ctcgctgccg agggtccgcg ggcactctcg tgccggacca
     2701 ctgtgatggt gccggtgccc gggcaggcgc gacggggctc caccggcact tccgaaccgt
     2761 cgtcgacaac gatgacggtc agatccttga ggacgggcaa taggcgttcg acgccggcct
     2821 ggttgtcctt gaccggaatc acgaccgtga catcggcagc ggacggcgtc gacatcggac
     2881 gaggattcgc gacacctgaa tcgagaaggt gtcgcgcaac cgcagcgctc tgctgatcga
     2941 ccacctcgag gaagccgtca ccgatcatcg ccgcagccgt cggcgccagc ttgagcatcc
     3001 gtgtcggaga tccgccgatg agtacccggc cacccgagta cgtgcgcacc ttggggtcca
     3061 accgaatgcc gaagccgtcg gggagtcttg ctggtcgcat cagatgatcg taagggacgt
     3121 agcccgtcgt gctgcaactc gtcgcagcct cgaaagccgt cgcaggtgga ccgtccgccc
     3181 ccactcggtc gtggtcgtca ccgaagcctc ccatctggac caggctccca ccgggccagt
     3241 ccatccgtca cgcgtcctac caactcaccg aagatgtatg cgccgtcttc ggcggtagcc
     3301 ccgactggat tgccgagaac gccgttttgc gacacagcga ccataccgcc cgaacgcatt
     3361 cctggcatca ggtccgcgat aggagacaaa tttcctgtct cagcaaggct catgtgcacc
     3421 aaatcgggcg aaaggtgcag caataggctc gtttcagtgt gtccggcatg cgcgtcggca
     3481 cccggaacag cgcacggcgc ccaagcagca tcacgcccct cataccgcag aagtgccacc
     3541 gctttggcca atgcgggacc atttccgccg tggccgttga cgaacagcac ccgcttcgtc
     3601 catcggaacg ccgaccttcc gtattcgacg acgacggttt ccatggcctc ggcaccgatc
     3661 gaaatcgtgc cggcaaaccc ttcgtgttcg ccgctcgccc cgtattcgat ggccggggcc
     3721 acgacgacgt cgggcagagc ctgtgcgact gctctcgcga tccgagtgtc ggtatcgaga
     3781 ggcaggtgcg gtccgtgttg ttcgaaagaa ccgaccggga ccacgacggt gagcgcagtg
     3841 ccctcgagcg caggactcgg gatggcagca aggtcacgcg gagaactcat cccccgatcg
     3901 tattggtgtg ggcccctccc accgcaacac tgcatgcgga tacaccgggg cattttgttg
     3961 tgccccggcg tattcgtcag ttgtcgcggt tcacgccgct gtcggtgggc tgttcccgcc
     4021 gatcggctgt tgatacggat cgatcgacat cggtggatag aacaccgatt tgcgatccgg
     4081 cccgatctcg acccgccatt cggtgtggtg cagtgtccga tgatgatgac cgcacaacaa
     4141 gatcagattg tcgagatctg ttgggccacc atcgttccag tgaacaatat gatgggcatc
     4201 gcaccacccc gacggtgtcc cgcaccccgg aaacgcacac ccaccatccc gaaccgcaag
     4261 agcccggcgc tgcggaaccg tcgccgttct cgcttccttg ccgcagtcga gcggaacacc
     4321 attcgcatcc aacaagatcc gcgtcacggt gcagtcgcac gccaacatcc gcgcagtgtc
     4381 gatactgatc gggcccaccc aatcggtcca cgcaaatccg agttcttcgg tggacggcag
     4441 gaggtctttc aacgccttga ggtcggtcat gtctttcgcg gacgcggtaa cggtgatgtg
     4501 tggtttcact ccgccttcga tcggacccaa ccccgcccgc tcgtaccggc gcagtagttc
     4561 gcagaacccg tccgcacgtc gtaatgccgg agtgcgggtg tctttggcgc cgtctttctc
     4621 cggcgtcgga gccgacagac gcgacaacaa tgtgatcaga cgcgccccgt tcacagcatc
     4681 taggtctccc ttcaccgaca cgcgcccgta caaacctttc gacgcgtaga actcgttacg
     4741 ctccgaatct tcccccaccg gaacgccgtc ctcacggttt ccgtactgct tgtcgatccg
     4801 ccggatcgcc gcccgcaccg catcacaatc agcaatattc tgcgacgcca ggccaagcag
     4861 gatcccgcga gccttgtcca catccgccag ggtcatgttc ttcggtggat gctgacaaaa
     4921 cgacgccacc tgccgcgcct tcaacgcatc aacgtcaccg cgatggaatg catccgcgac
     4981 aagaggttcg agcatcaaca accgcgccaa cccgacgagc aggctgcact caccgatctc
     5041 cagattcgtc gacccgtgca accactgcgc gatcgccgaa aacccttccc gccgagaacc
     5101 tcacgggaaa acatctccac gatcagcaac accatccgcg actgcaattc gtgtcgggcg
     5161 cgcaccagat cgagaacctc actcttgagg cccacgccat ccagttgcca cgcttcccga
     5221 ctgtccatgc tcaagacata ctttcgagaa ccagggacaa aacggacgac tatatcgcca
     5281 acgcggcccg atgcccctcg atgacggccg cgtgcgaacg cctcggagac aaggcgtcac
     5341 cgatgcgaaa tttctcgaaa cagcttccct gcaaggtgtt ccacaactcg tcgacaggtt
     5401 cctgctgtgt cgcgaagacc acccagtcga ctgcaacttc ctcggtcaca cccgtcggaa
     5461 ggtgaaccaa gctcacgagc ggcgaggccc cagcactcac cgaagtcacc atcagatctg
     5521 tccgttgcac gatcgacttc tcgaacgccc ggcggttcca cccctcgaaa tccagggtga
     5581 tgctcaggtc ctgcgcgacc accatgccgt tggtcgcgat cgtgacggca cagcctcgat
     5641 cagcgaggaa ctcagccacc gacgtacctt gatggaatcc cagttcgtcg tagacgagca
     5701 ccgttccact cggcagaatc ttgccgtcga gaacgtctcg aacacccacc acacgctctg
     5761 atccacccgc ccacgctggg cgtaaaggcc gcgctccggt tgcaatcacg accacctcgg
     5821 gcgcgcgctc ctccacgatc gcgagatcag ctccagcgcc cagctcgatc gtcacaccgc
     5881 ttcggcgaca atcagattcg agattccgga cgaggtcggc gagctcacgg cgagccggta
     5941 ccaccgaggc ggtaagaatc tgtcccccaa gtcttcgttc acgctcgaac agcgtcaccg
     6001 catgacctag ctgggccgct gtagccgcag cttgcaggcc ggcggggccg ccaccgacca
     6061 ccaccaccgt gcggccccgt cgccgcggca tcggcagcgg taccgattcc cgaccgacgc
     6121 gcggattttc ggtgcacccc aaccaacgtc ccagacccat ccgcccgacg cattcctggt
     6181 tgcaggacag gcaggttcgc acctgtaccg attgaccagc acgcgccttg ttcacaaaat
     6241 ccggatcggc gatctgcccg cgaactactc ccaccagatc gcatacaccg tcctcgagtg
     6301 cggcttcggc ttgctccggc gtcttgaaac ggccgactcc gacgactgga agatcgacga
     6361 cctccctgat cgcagacggg acgaagttgg cgtagccggt gggtacagcc atcgatgctt
     6421 cgaccagatg cagtgtttcc gtggccattc cgacggtggt gttgatgtag tcgacgtaac
     6481 cgtcagcttc gatgagttga gcggtggcta ctgcgtcgga cagttcgatg ccaccccgcg
     6541 ttccttcgta tccactcagg cgcacaccga ggacacgatc cgggccgatg gccttacgca
     6601 gtaccccgag cacttcgaga aggaaccgtg cacgatgtgc cacgcccccg ccgtagcgat
     6661 cggtgcgcac gttggtcgag ggggcgagaa acgcgcgcac tatggacgac tgcgacgcct
     6721 gcaattccac cccgtcgaag ccaccggcga cgcaattgcg ggcaacaagg gcgaaccctg
     6781 cgagaatctc ggcgatgtcc gcgtcgtcga cagctttggg cacctcgcgg aacaacggat
     6841 cggcgacggc gctcggcgcc aacagcggcg atcgcgaata ggttccagcg ccctggcccc
     6901 cattgtggtt gatctgcgcg agaatcgacg aaccatgtgc atgaacggcg tcggtgatcc
     6961 gccggtatcc ctcgatcgct tcgggccgat agcccgcaat catcttctcg tacggtcgat
     7021 cccaaggatg cgtcgagtgt tcctcggtga tgatcagacc cgcaccgccg cgtgcccgcg
     7081 cctcgtaata gctcgcgtgc tgctccgtcg gcaacccgtt ctccgcgtaa ttggtcaggt
     7141 gtgcggagaa caccacccga ttgcggagtg tcaggttgcc caggcgcagt ggcgcgaaga
     7201 ggcgcgggta cggcgtcatc agaccttgga gtttccttga tcgagcgcga tatgacttgc
     7261 cgtgacggtg cgggaaccgt caccggcgag ccacaggacg gtatcggcga tctcgtcagg
     7321 ttcggcgaaa gcgatgtccg agaggatcgg agtgaagttg ggaaggtact gcggaaactt
     7381 tgcaaatacc tcgagagctc cctggtcgac ccccatcggc gtattcactc cgtaaggatg
     7441 gatcgaattg accctgatct tgtactcccc cagttccttt gctgcggcct gcgtcagacc
     7501 gacgagaccg aacttggcgc tcgcgtagtg cgcctgtccg ggcagcgcct tgagtccggc
     7561 caccgaactg accacgatga tcgatccacc gcgcccaccc tcgatgatcg ccggcaccga
     7621 ggccttgagg gttttccaga ctccggtcag gttgatgtcg atcagagttt cccactgctc
     7681 gtccgacatt tcccagaatc gattccagtt gcagaccccg gcgttggcga ccacgatgtc
     7741 gagtcgtccg aactgttcga cgccgtcctt gaccaccgcg gtgagcgctg cgccgtcacg
     7801 gacatcgacc tcgcgggcga ggatcttccc gccctccgct tccaccagac gtatcgtctc
     7861 ggcaaagtcc tcggaagttg ctgcgtcgta ggaattgtcc gacgccaccg acgcgcatac
     7921 gtcgaccgcg atgatcgctg ctccctcgcg cgccatcttg accgcgtgcg cacgtccttg
     7981 accgcgcgct gcgccggtga tgaaagcaac gcgaccttcg agcgaacgaa ctgaaccctg
     8041 tgtcatgaga tagccctctc tgcagaattg aaactcgttc tagtagtaaa ccctgcaaag
     8101 cgcccttcgc ggatggattc gagaacagtt ctcggggcaa ccgagtcgcc cacctgaatg
     8161 tcggcctctt ccttcagcgt ttcgttcggc agacgaggcg agcagtcgat caccacttcg
     8221 cactcgagct gtgaccgctc gccggtatgg acgtgttcga cgcagacggc ccctcgagcg
     8281 atttcggtga cacgtgacgc gagcaccctg gtgatcccgg cctgctggat gcgcgtgttg
     8341 gcgccgacca gatccccact cgcgccgagc ctcgaaccga cgatgctgtc gggagtcaca
     8401 atcgagacgg agatgccatg ggccgcgagt gcttctgcga gcgccaccgc gatcggtcca
     8461 ccgaggggat cgatcagcgc gaccggaccc ggccccacgg tgctcaactt ctcgaaggct
     8521 tccgacgcat ccaaccaccg aaccgaatcg tcgaccggat acgtcggcgg cctcgcagca
     8581 ctaccggtgg caacgacaac aaactcgccc cggctgcgtg cgtcacccag gtcttgggcg
     8641 tcgagcgccg ccccggtccg catcgtcacg ccgctacgac ggcattcgtc ctccaaccac
     8701 tgcgcgagga gtgcgaaccg gctacggccg tcacctcgcg caaaagttcg gatcatgccg
     8761 ccgaactgcg ataaactctc gacgagtgtg accgagtgcc ccgccaccgc cgagatccgt
     8821 gcggcttcca tcccagcggg gccacccccc acgaccagca cggctcccgg tgtcgaaatc
     8881 tgctctcgct catcggggtc caccgtctcg aaacccgcgc tcggattccc gacgcacgtc
     8941 acgacaggat tgcgaggatc acgcacgagg cacgtctgat tgcagagcac acacggccgg
     9001 ggacgatcgc cgcgcgaggc tttcacaacc aggtccgggt ctgcgatctg ggcccgggtc
     9061 atctcgacga actcacctac ttccagcgcc gcgtgagcgt cggcgatgtc cacgacgctc
     9121 ccctgcacga caacaggaat cgacagagcg accttgatct cacggcacag atcgaggttg
     9181 aagttcggcg ggcagtgaaa atcggggcgg taccggtcag acgaatagag tccgccacga
     9241 acgacgacaa ggagatcgag gaacggagac acttcacgcg cgataccgat ggcaagatcg
     9301 ggcgtcaggc ccgcccatgg agcttcctcg tcacaggaca accgcaggga gacaacgtga
     9361 tccgacccga cttcctcgcg cactgccgag atgacctcgc gaacaagagc aacgcgatcg
     9421 ctaccgtatc ggtcttgccg agtgttggtc agacccgaaa ggaattgtcg cagaatgctt
     9481 cgaggaccgg catcgagttc cactccgtcc attcccgaag cgacggccag cgccgacgcc
     9541 tcacggaagc cgactatcag ctggtcgatt tccggctgcc ccattgccat cggcaactca
     9601 cgcgtcgccg ggtcggccac tcttgacggt ccccacagca ccgactggga gtatgcgctc
     9661 gatccctgca tgccggtatg accgagcccg gccagaacca gcgttccgta cggagcacat
     9721 gcagccgcga tcgactgcca ccccgggcca cattgcgacg ccaacggcgc ccgctcgtac
     9781 ggccagtcgt tctccgatac cgaggccact tcggtgacga cgatcccggt tccgccctcg
     9841 gcgcggcgct ggtagtacgc acggtgccga tcggaaagcg agcgtcccac agcaaggttg
     9901 gtctcgtgcg gaccgaaaac aaccctggac cggacgaggc gtccggccag ggccgtgtgt
     9961 tcgtcgagtg tcaactcaca gctctatcgg agccgtaatc tcagctggca cccaatgtgc
    10021 gtgtgaaccc ttccggtatc accaggtccg ccgggctcag ttcggtgatg gacgagtggc
    10081 cgaggcccat cagccccgag tcgatgccca tccgcatgag gtcgagcacg ttctcgacac
    10141 cggcctggcc gttggccgag aggccccaca ggtaggcgcg cccgagcatg acggccttgg
    10201 ctccgagtgc gagcgccttg acgacgtcgc cgccgcgtcg aatgccgccg tcgagaacaa
    10261 cttccacctg atcgcccacg gcctcggcaa ttcccgggag cacacggatc ggcgccgggg
    10321 tgccgtcgag gttgttacca ccgtggttgg agaccgagat tgcggagaca ccggcgtcga
    10381 cagctcgctt ggcgtcgtcg atgcgcatga tgcccttgag catgaacggt ccaccccact
    10441 gctcacgaag ccaggcgatg tcttcccacg tcggaagcgg agtctgcatc cactcgccgt
    10501 atgcgccgaa gaacgtcggt gcaggctggc cgggagccgc gaggttgggg gtggtcaggt
    10561 ccgggatctt gccggtcttg gcgaactcga gcaaccactt cgggcgcatg attccctcgg
    10621 gtgcgaactg gaacatcgcc ttgaggtcca tcttttcggg gatggacggg ctgccccagt
    10681 cgcgaccgta cgagaaggac cagtcggtgg tgatgatcag gcccttggca ccggcagcgc
    10741 gagcgcgctc catacgctgc aggagcacgt cgcggctgcc gacccaatac atctggaaga
    10801 agacctgcgg gttggccgcg gcaacttctt cgatggactt gctggcgaaa gaactcagtc
    10861 cgatggctgt tccccgtgct gcagccgcac gggctaccgc tacttcaccg tcggggtgca
    10921 ctgcctgcac accggtcggt gaaatcatga cgggcaacga aatgtcctgg cccataatgg
    10981 tggtggacat ctcgcgcttg tccgacagcc cggcagcgtg gggagcgaaa cccaattcgc
    11041 tgaatgccgc gacgttgtcg tcgaccgtca gccctttctc ggatcccgct accaacgccg
    11101 cgtacacgga cttgggcaac ctcttcttcg cacggcgctg tgcttctgcc accgtctcga
    11161 agaaagcatt cttagccatt gaactgacct ctctggggtg cagctttcgc cgcactgtcg
    11221 gtatcgcgca gtagttactg cattcccgca agcgggtttt cgtcacaaat cttggcgggc
    11281 ggacgcatca tcagtgtcag tggcaccgat gcgcgtgggg tcttacgttg accggaacgg
    11341 gagtggtcga cactggactt cgggacctcg cccgccgacg ccagtgccat ttcaccgtta
    11401 ccgatgacgc actcggggtc aggtccgtcc atcggcagtc cggtgaagaa cttggccgcc
    11461 atgcagccgc cgcggcagga gtcgtagtgg ttgcacttgc tgcaggcgcc accggtttgg
    11521 ggcgagcgca gctcctcgaa caggtccgag tgctgccaga cttcctggaa tccaccgtct
    11581 ttcacgatgt ttccggcgag gaactgctcg tgaatcgcga aggggcatgc gtacacgtca
    11641 ccgaccgggt cgatcaggca cacgacgcgg cccgcgccac agagattgag accgggcagt
    11701 gcatcgccga aggcggagag gtggaagaac gagtcgcccg tcaggacgcc ttcgccattg
    11761 gcgaccagcc agttgtagag ctcacgctgc tgctccggac gcgggtgcag gtcgtcccac
    11821 acgtcggcgc cgcggcccga aggacgcagt cgagtgatgc gcagggtcgc gtcgtacttg
    11881 tccgcgagag ccttgaactc gtcgagctga ctgacgttgt gccgcgtgac gaccaccgaa
    11941 atcttggcgt ccttgaagcc ggcttccttc aggttcgcga gagcgcgctc ggccatcgca
    12001 aacgaaccag gcccgcgcac agcgtcattg acctcagcgg tggcaccgtc gatggaaatc
    12061 tgcacgtcga cgtagtcact ggctgcgagg cgcgccgcga ccttcttgtc gatcttcacg
    12121 ccgttggtgg agaacttcac accaacctgg tggtcggtcg cgtagtcgac gagctcccag
    12181 aagtcggagc gtaccgtcgg ctcgccgcca ccgatgttga cgtagaaaac ctgcatacgc
    12241 tgcagctcgt cgatgatgga cttgcactgt tcggtgctca gctcgttggg gtcacgacga
    12301 cccgacgacg aaaggcagtg cacgcaggac aggttgcagg cgtaggtgag ttcccacgtc
    12361 aggcagatgg gggcgtcgag tccgagttcg aactgatcga cgaggcgacc caccttggcg
    12421 ggcggggagg gctgttcaag aactgaggtc atggctgtgt cctcgcgaag aaatgaagcg
    12481 ggcgaacgtg cggaagggct gttccgcacg tgggggcgag cgtctggctc agacaggcac
    12541 gatcatgtgc gagtcggcga gagtgctcag cgcgcgggca tattggggag ccgcgtcatc
    12601 ttcgatgcct tgcgcacgta gtgcggaccg gacatcagga tggtctggca acgccttgac
    12661 caccgcaacg atggtcgtgt tcttcaggaa cgacagcttg cgtgttccga agtggtagag
    12721 caacgccccg aaaggttcgg ggcgaagcgc tacctgcggg tggagtttcc accccgcatc
    12781 gagatccaaa gcagacacgg tcagtacacg ccgcacatgc cgtcgatgga aacttcctcc
    12841 acgagggact cctcgacgag gtcgttctcg agcgccttgt tgtcacgatc tgacatggat
    12901 tcctcctgcg gtcgcactcc gtctggaagt ctgtagatca gacttccaac ctgggagttg
    12961 gtggtcacta gtgggacttc actgtgactc ggttcaccac tgtaatggca tcgagtgcag
    13021 aatgacacac atggagcgaa aaaagtatgc gtagccgcaa gaaaccgtcc gctcggatag
    13081 gtcgtcgccc ctccacaacg aaggagggaa tcagcacgat cgggatcgaa ctgttctcga
    13141 cagtcggctt caacgaggtg agcgtcgacc agatagccga cgccgccggc atcgcgcgtc
    13201 ggaccttctt tcgctacttc ccgtccaaga acgccgtccc ctggggagac ttcgacggac
    13261 atctcgacga gatgcgcgag cacctggatt cactgcccgc tgatattccg ttggtcgacg
    13321 cactcgaatc cgcattgttg acgttcaaca ccttcccacc ggaagagact gcggtacatc
    13381 gcaaacggat ggaattgatc ctcgaagttc cgacgcttca ggcatactct gtggtgatgt
    13441 acgaaggttg gcggcaggtg gtcgccgact acgtcgcacg ccgaatgaat ctcgagccga
    13501 ccgatcacgg cccacgcacg gtcggctatc ttctgctcgg ggtcgcgatg tcggcgtacg
    13561 agcagtggct caccgacgat tcactcgagc taccggaact cttgaaaaca ggtacgcagt
    13621 ccctgcggac cggtatcagc acctgagaaa gcatcaccac ctgagaaagg cgatcatgtc
    13681 caccatcacg aacgaacccg tcctggattc cctggcgccg tcgacgccgg gcttgtggtc
    13741 gttcgggcat gtcaccgtcg aacgcgtcga gtccggtccg atctcgctcg atcgccgagg
    13801 cggtcgcctc cacgtcaggc actccctcac tcccgccgat ctgagtgagc gactcgtcgc
    13861 cggagtcacc tcgtcgatcc aggacgcgga gttcggtcag gacgaattcg agttgacgat
    13921 ggtcggcctg gttcgctcca gcgtggacgg cgctctcgaa tcctggacca cctactaccg
    13981 gaactccctt gccgaactac tggacggcac tgcagatttc gcgccgattc acgacaaagc
    14041 cgaagagctg gtccgcggtt ccgtgctcga tctgggttcc tgcttcggtt tcctgcccct
    14101 gcggctcgcg cgcgccggaa tggacgtgac cgccacggac atcctgcccg gaacgatgat
    14161 cctgctcgac gcggttgccc ccgaactcgg catcacgatc gacacggtga tctgcgacgc
    14221 gggtgaagta ccggcaccgg acaacagcgc cgacaccgtc accgcgattc atctcctcga
    14281 acacgtcgac gacgcagtcg gcgaggccgt gatcgcgcaa gcactccgga tcgcgcgtca
    14341 acgggtcgtc attgccgtcc ccttcgaaga cgaagccacc gcgtgccacg gccacatccg
    14401 cactttcgat ctcgagtcac tgcgtgcagt cggccggcag agccgtgtgc cgttcgaggt
    14461 attcgagcat cacggcggct ggttggtgct ggacgcatcg gccgcctgaa aagaactagc
    14521 cttctttcag tgtatgggcg acaagcgcat tcgcgtgccc atgacccata ccgtgctccg
    14581 acttcaacca gttgaccaat tccatgtgct tcgtgaggtc tgagcttctg atcagctctt
    14641 gccattcacc gatcggccga ccgtacttct tctcgatcga cggaaaatac gacgccgggc
    14701 ctttcacggg ttcagtcatg accggaatct acctccaccg cccgacaata ttcgcgcacc
    14761 ggcggcgccc gcatgtccgc acctgcggaa tcgctgggta gacacgcgac catcgcatta
    14821 tggtcgaaga acacccaccc gagagcacag gagttcagcg atgcgactgt ccacacgaaa
    14881 tcaactcacc ggcaccatca ccgaggtcaa cctcggcacc gtcatggcga cggtcaaggt
    14941 gaagttggac ggcgggaatc agattgtgac ctcgtcgatc acccgcgatg ccgcactgga
    15001 tctcgatctt gaaccaggcc aagccgcaac ggtattcgtg aagtccaccg aggtgacgat
    15061 cggcgtcgag taactggcga gcgatgtgat cagggccggg tgagatgcgt ggtcatcgtg
    15121 tagtccttgc tcggccccgt cacggtccag acgacactcc aggtcgatcg atctccggca
    15181 gtgacgtcga ttcgcccgcg gtaggtatcc gcaccgcaca ggtgctcgac ttcctccccc
    15241 ggcgcccagt cgtggaactc tctgccgtct tcgaatgtca cggcccactc gaggccgcgc
    15301 tcggcaacga acagagttcg gaacacgggt aattccaggt cgccgtcacg cagcgtgccg
    15361 ctctcgtacc agcgcatccg accgtcaccg gcgtcggaga tagtggtctc gccgaataca
    15421 tgcttggtgg aaccggaaag gcggtcgacg atgtcccgct cgaagtgcca ctttcctacc
    15481 agcgccctcg gctcgatttt cgcgtcgtcg accatgtggc gattctagtg accctgcatt
    15541 gcccgacgac accggagacc gactcgaaca cactgcgaga gtatgttccg agtacacccg
    15601 gaacacgcag gagattttca caaccatgaa cgattcggcc gcccttcaca aacgcgcagt
    15661 gttcgccgag gatctcgaga tcggccaagt gatcccattg ggttcgtatt cggtgaccga
    15721 agaggaaatc gtcgagttcg caactcagtg ggatccccag tggtttcacg tcgacaagga
    15781 agccgccgag tccggcgtct tcggcgggtt gatcgccagc gggctgcaca ccctgtcgat
    15841 ctgccagaag ttggtggtcg ccagtctcta cgaccagtgg aacgtgatcg ccgggaaaac
    15901 catgcgcgac gtgagcttcc tccgtccgct tcgccccggt gacaccctga gcggaaaggt
    15961 caccgtcgac aacgtcgtgt tcgacaatcg cgcgcgagga ttggtgacca tcacagcgaa
    16021 attggtcgat gcacacggca atcgcgttct cgacgtggtc actgacgcct acctgcactg
    16081 ccgctcgact cgcggacagt gaattatcgg taaatttcgc accacactgt ccgaattgcc
    16141 gtactttcag tgacgttgcg acgcaacaat acccatccct gcaatcagga gttcctcaca
    16201 catgagcagc ctcggcgaga tcaatcctct cggcattctt cagatcctca tctacatgct
    16261 cttcggtgga tcgtccagca ctcccgaata gaccctagct tgccatagcc atagtcagca
    16321 tctagggtgc tgactatggc aattactagt caggtatcag tggcggccgc gccgtcgacc
    16381 gacgcagcgc gtcatcacac cgaattcgac tccagttggc ggagtttcga cggcatccag
    16441 ggcggactcg caattgcacg gatgctgcga gtggcggccg acgcgtccgg agccgaaccg
    16501 ctggcagtca cggcgcacct ggaccgcccc gttcctcctg ggccggccga gttgactgtc
    16561 ggcgagatgc ggggcgggcg gaccgtcagt tgccacgtca cgctgaccgg ttcgggatcc
    16621 gctctcgttc ggttggcccg cgacatagga gctacaacaa tgtccgcccc tgtcgccgaa
    16681 ccggctccgg ccgacccgtc aaccctgccg ccgctggaga tcccggtgga ttttgtgccg
    16741 ttcgcccagc acctcgacat ccggccgatc aattccgcga ggccgttcgc cggtggcgac
    16801 tcccctgaat tcgacgtctg gatccgcctg ctcacagacc tcgatttcac ggcccaagag
    16861 cgtgccgcgg tccttctcga cgcgcttccg cccggactgt tcgcgacact gacgatgccc
    16921 gtggccatac cgaccatcga gttcaccgcg catttcacac cggagcacct cacggcagat
    16981 ccccgaacgc tgtcaccgtg gtatcgccta cgccacggga ctgcctgggc gacggacact
    17041 ctctgtgttg acgaatccga actattcgac tcgaccggac gacttgctgc tcgggctcgc
    17101 cagcttcgtc gaattctgag ttgagtttca gctctcctac acctggaggt cgcgagcacg
    17161 gaacgcaaca agcgatgctg tcgaaagcac cactgccaca acgatcattg ccagcagtgg
    17221 cagaactgtc accggctcgg caggggcacc cggcacgtgg gtgaagggcg acagatccag
    17281 cactacctgc ggaagcttca gcaacgtgcc gagttgcccg agcacgatgc atgccgccaa
    17341 gattccccag gagagcgccg acgcgtacgt cggaaacagt ccgaaagcca acgacaccaa
    17401 ccccacgact acgagcaccg caggcagata ggccaacgcg gcgccagtca tgcgaagtgt
    17461 gttgccgccg atgtcgtccg tgacgagccc gaaggcaaaa ccggtgaagg cacccatcaa
    17521 gacgtgcgcg aggatcacgc cgcccacaac cacacccagg tgggaaagca tccacctcca
    17581 ccgcgacaaa gacgttgcca gcaacggttc gagccgtccc gatgtttcct cgccccgcat
    17641 ccggagaaga ctctggaccg aatacgccgc gatgactatc gacgtgagcc ccagagcagt
    17701 ggccaagtag gaatccacca gatccgatcc gccgccgcca agtttctcga acatctccgc
    17761 ggtctgctgc gacgatccca ccatcgcgtc gatctcgttg cccacagcgc cgtacgcgaa
    17821 cccgagtatg acaacggcaa agatccacca gaacatcgtc attcgctgca tgcgccacgc
    17881 caggccccag gccgacggta gccacgatgc cgcgactggc gggccgggtc ggggctgaac
    17941 gagtcccgct ccctgatcgc gatggccgat gagaacaaaa gccaccacca cgtgcacagc
    18001 cgcaaatccg agcagcaaca acaggatcca ccaggcgtcg tcgtcgaagg gtcggatctg
    18061 ttgtgcccac ccgatcggcg agagccacga caaccagcca ctgaccacgc gagttccgtc
    18121 gtcgacgacc gtactgttca cgtcgccgat cgcccgcagc atgaaggcaa cgccgatcgc
    18181 ggcacccgcc aggccgttgg ccgtacgagc accgtcggtg atctgcgccg tcacggctgc
    18241 cactccggca aacgcgattc cggtcccacc tatcgaggca cccaacgcga acgaaccgac
    18301 gacaggcaaa ccgacggcga tcgatcccag tgcaattccc agtgccagaa cgacgttcgc
    18361 accgaccgtt acgatcagag cggaggtcag gagcgctcgt ctacctacca ccgctgcacc
    18421 gatcatctcg gctcggccgg tctcctcgtt ctgtcgcgta tggcgaacca ccgcgagggt
    18481 gctcatcaat gcggccacca acgcgaacac gagcaccgac tggctggcga ctatcgcgcc
    18541 ctgcgaatca ccggacacga tcccgttggt cgccagcgcg accggcgata ccgcggaact
    18601 gactgcgatg tttcgtaatt cctcgggtgt cgagtacagc ccggtcacac tggcgacaac
    18661 cagcagcatg aacaagacga tggagataac ccacacggag agctgaattc ggtcccgccg
    18721 caaagccagc cgaaccagtc ggcgtgtgcc ggtaaattga cttgccgagg ccggtaccgg
    18781 tggagcggcc tcgacaacaa cggtcacggc cgtactccgg tctcggctcg caggtgtacc
    18841 gaagtctccc tggcccgttc gtcaccgtaa tgccgcaaga aaagttcttc gagagtcggc
    18901 ggagtggaag tgagcgccac gattccgaat ccgaccaagt acgtcatgag tggtccgagt
    18961 tgcgcgctgt cgacttcgca ggtgacgtgg ttaccgtcga tcaccgcgtc gtggacaccg
    19021 ccgagtccgt cgagtccacc gatcggaagc tgcgtctccg ccgcgacgga cgtgcgcgtg
    19081 agatgcctga gttcggacag tgttccctct tccaccgtcc gcccgtcgcg gatgatgctc
    19141 acacgatcgc acagcgcttc gacctcggcg aggatatggc tcgacagcag taccgtcttt
    19201 ccggcgtgag ccacctcctc gacgcagtcc tggaatacgg cctccatcaa cgggtcgagc
    19261 cccgatgtgg gttcgtcgag gacgtaaagg tcgacgtcgg acgagaacgc ggccacgagt
    19321 gcaaccttct gccggttacc tttcgagtac gtgcgcgcct tcttggtcgg gtccaattcg
    19381 aagcgatcga gcaactcgct gcggcgtttt cggtccaggc caccgcgcag gcccgccaac
    19441 agatcgatag cctcaccgcc ggtgaggttg ggccacagat tcacgtcgcc aggcacatag
    19501 gcgagacgcc gatgcaaagt aacagcgtct cgccacggat cgccgccgag aacggtcgcg
    19561 tctccggagt ccgagcggag caggccgagg agcacccgaa tagtggtgga cttgccggaa
    19621 ccgttcggcc ccaggaatcc gtgcacttca cccgtcgaga cgtcgaggtc gaggccgtcc
    19681 agcgcgcggg tggtaccgaa ggtcttgacg aggcttttca ctgaaattgc cgcacccatg
    19741 aattttaccg tacgccgatt tcacaaatgt gtgaagatag aaaagacgga aagatcgaag
    19801 ggttccgatg accgacacca gccacaccgt tgcaccggac gagagccgac actatgtcga
    19861 gcgactctca ctcgtactga gcgacatggg ttttccgccg atgcccgcac gcgtctgggc
    19921 cgcgttgatg accagcgaga caccctcgtc cacgccagga gatctcggat ctcggttggg
    19981 agtgagcccc gccgccattt ccggagccgt cagatacctc cagcaagtcg gattgatcga
    20041 gcgtgtggcc gtccccggat cgcggcgtca gcactactgc gtcagcgtcg acatgtgggc
    20101 cgacgcgttt gtcacgaaac agtcctcgct ctaccaggtc tcggcagttg cggacgaggg
    20161 catacgagtc ctcggtgaag gaaccacagc cggcgatcga atggcagatc tgcgcgactt
    20221 cttcgacttc atggccgcgg aaatgccgcg aatgctcgcg gactggcgct cccggcgaaa
    20281 agaccggaca cctggcaggt aacttcaggt ttacccgcaa tttcggctgg gtacctcaat
    20341 tctcgttcca gcatggtcag gtgcggtgta atcgaggagt tgctgatgaa gctgtcgatc
    20401 aggggcgcga acacttcacc gactccaccc ttcgcgcgta ccgcttcacc cgaaacctgt
    20461 gaacgatcga tttccctgaa tcgcaccgcc gccgaggcat tcgccggtga tcaccatgca
    20521 ttgtcgaaac tactggcccg gttgcaccct cgcgtcgttc tctactatcg cgccagactc
    20581 ctcgggacgc ccgtcgcgac gttcgccgac gatctcgccc accaatgcac cctggacgtc
    20641 gtcacacgaa tgtccgacac gatctcggcc gacacacgca tcgagacctt cgcgtacgag
    20701 ttggcctctc gacgaattgc gtcaattccc cagtcacgca acatcgacgg acaattcaaa
    20761 cgcccgcgcc gcctgaccga cgaccaacgt gaggttctgg tactacgaat cgcagtgggt
    20821 ctgactgccg agatgaccgc atcgactctg cgcacaactc gttcgagcgt gctcctccta
    20881 cagcatcaag cgctgcgcgc ctggacggtc tccgagcaga gctagatcag accttgctta
    20941 ctcgcggcgt agacggcctc tgctcgacgg ctcacctcga gtttgcgcat gatgttgctg
    21001 acgtgaaact tgacggtggt cggtgagatg aacaatctct caccgatttt gatgttggac
    21061 aagccgtttg cgaggagtct gagcacttcg agttcgcgat cggtcaatcg ttctttcgac
    21121 gacgactgcc cgttgagcga tcgcacgaca gctgacgcgc tgcgcgaatc gaacgcactc
    21181 tcaccccgcg agaccgccct gatcgcacgc accaattcgg tggtgtcgac gtctttgacg
    21241 acatagccgc gagcacccgc gtgcaccgct cgcaccacca gttgatcgtc gagaaacgtg
    21301 gtgagaacca acagtccgag gccgggatgc gccgaggaca gctggccaca cagggtcagt
    21361 ccctcgtagt cggaactggc ggagagtttg agatccatca ggacgatgtc cgggccgacc
    21421 ttgtcgacca cggcgagtgc ctcggcgagg gacgaagcct cccccacgat ctcgagatcg
    21481 ctttcgcgct cgagaaccga gcggagccct tgccgcagaa ttgcatggtc gtcgaccagg
    21541 acgattcgaa ccaatccctg aaccggcgat gctccgcggg tgatgacggg acgcgcagtg
    21601 gtcatgattc ctcgctctcg atttcagttg tggagcaaag gttttccatg cccgagtcga
    21661 acggcagtgg tagtcgcacg agaatctgaa tcccaccgat ccgcgcgcgc cgtacttcga
    21721 gcgcaccacc caactcgagt gttcgcgcct gcatgttcgc gagaccgcga tggcggccgt
    21781 cgacgtcgtt ggtggctgcg agtttgagga tcagacgcat tcgcgcaggg tcgccgtcac
    21841 cgtcgtcgga gaccgacagc aacacggagt ccgcccgata ggtcagacgt agcaccgccc
    21901 gattcgcgtg agcatggacc gctgcattga agagagcttc ccccgcgatc cggagaagag
    21961 cgtgttcggc atcgcccgga agttcggtcg gggtaccgcc gacgcgaacg ctcaccgcca
    22021 actcgtccgg catgtgaacc acgccgagtt gctcgagcat ctcgggcaac gacgtccggt
    22081 ccgcgtccga cgaatgattg agcgcgtaga tcgccgaacg aagctgctcg accgccctcc
    22141 tggtcaaatc ctttgccaag tccagtcttt cactgcgttc agcctccggg atgtcactcc
    22201 ggcagacctc gatctgcatg ccggccgaca acaccgcctg tgtcacactg tcgtgcagtt
    22261 cgcgggcgat ccggtgacgt tcgtcgtcga ccacttggtg ccggtgcgcc actccgagtt
    22321 gacgctgagt ggactccaat tcgctgttgc gcaccgcaag gtcggctgca gtacgattgg
    22381 cctccgcata cgcatcttcg gcacgctcga gcagtagttg tccgcgctga tagagcgccg
    22441 aattctgcag agccacagca gtctggctgg ccagaatgct gagcacggct ccgtccgtcg
    22501 tgtcgagggt tcggttggcc ggcgtccagg ctgcaaaggc acctaccacg ccgccaccga
    22561 gcacgatcgg cacaaacgcg tagtgcgcgt cgatcaccga gttggattcg attcttccgt
    22621 accggatgtc gtcgaggcat tcgacgacat cctccggaag tggcggtccc tccacattgt
    22681 cgaccagaaa aggggtggcg tccgggccga gaaccagtcg ccgtggtccg gcgtcgggca
    22741 aagttccgtc agccaaggcg aagacaatcc agttggcact cagatgcgca cgggcagctt
    22801 ccgcgaccgc gcagac
//
