LOCUS NZ_JARDXE010000008 22816 bp DNA linear CON 01-APR-2024 DEFINITION Rhodococcus qingshengii strain HQ HQ1_scaffold8, whole genome shotgun sequence. ACCESSION NZ_JARDXE010000008 VERSION NZ_JARDXE010000008.1 KEYWORDS WGS; RefSeq. SOURCE Rhodococcus qingshengii ORGANISM Rhodococcus qingshengii Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Nocardiaceae; Rhodococcus; Rhodococcus erythropolis group. REFERENCE 1 (bases 1 to 310594) AUTHORS Hu,J. and Xu,J. TITLE A novel hydrolase synthesized by Rhodococcus erythropolis HQ is responsible for the detoxification of Zearalenone JOURNAL Unpublished REFERENCE 2 (bases 1 to 310594) AUTHORS Hu,J. and Xu,J. TITLE Direct Submission JOURNAL Submitted (23-FEB-2023) Grain and Oil Risk Assessment Laboratory, Jiangsu Academy of Agricultural Sciences, No. 50, Zhongling Street, nanjing 210014, China COMMENT ##Genome-Assembly-Data-START## Assembly Date :: MAY-2022 Assembly Method :: ABySS v. 2.1.5 Genome Representation :: Full Expected Final Version :: Yes Reference-guided Assembly :: GCA_002079265.1 Genome Coverage :: 470.0x Sequencing Technology :: Illumina NovaSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI RefSeq Annotation Name :: GCF_029076895.1-RS_2024_04_01 Annotation Date :: 04/01/2024 06:35:53 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 6.7 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total) :: 6,927 CDSs (total) :: 6,855 Genes (coding) :: 6,768 CDSs (with protein) :: 6,768 Genes (RNA) :: 72 rRNAs :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs :: 1, 1, 1 (5S, 16S, 23S) tRNAs :: 66 ncRNAs :: 3 Pseudo Genes (total) :: 87 CDSs (without protein) :: 87 Pseudo Genes (ambiguous residues) :: 0 of 87 Pseudo Genes (frameshifted) :: 33 of 87 Pseudo Genes (incomplete) :: 63 of 87 Pseudo Genes (internal stop) :: 12 of 87 Pseudo Genes (multiple problems) :: 18 of 87 ##Genome-Annotation-Data-END## ##antiSMASH-Data-START## Version :: 8.dev-cf2fc5ee(changed) Run date :: 2025-09-12 19:34:22 NOTE :: This is a single region extracted from a larger record! Orig. start :: 49871 Orig. end :: 72687 ##antiSMASH-Data-END## REFSEQ INFORMATION: The reference sequence is identical to JARDXE010000008.1. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ FEATURES Location/Qualifiers region 1..22816 /candidate_cluster_numbers="1" /contig_edge="False" /product="redox-cofactor" /region_number="2" /rules="(cds(PF04055 and (TIGR04085 or SPASM)) and (TIGR02111 or TIGR03966) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]))" /tool="antismash" cand_cluster 1..22816 /candidate_cluster_number="1" /contig_edge="False" /detection_rules="(cds(PF04055 and (TIGR04085 or SPASM)) and (TIGR02111 or TIGR03966) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]))" /kind="single" /product="redox-cofactor" /protoclusters="1" /tool="antismash" protocluster 1..22816 /aStool="rule-based-clusters" /category="RiPP" /contig_edge="False" /core_location="[10000:12816](-)" /cutoff="20000" /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and (TIGR02111 or TIGR03966) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]))" /neighbourhood="10000" /product="redox-cofactor" /protocluster_number="1" /tool="antismash" proto_core complement(10001..12816) /aStool="rule-based-clusters" /tool="antismash" /cutoff="20000" /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and (TIGR02111 or TIGR03966) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]))" /neighbourhood="10000" /product="redox-cofactor" /protocluster_number="1" misc_feature 149..164 /note="TFBS match to CRP, Cyclic AMP receptor protein, confidence: strong, score: 18.06" /tool="antismash" gene complement(225..1706) /gene="mftG" /locus_tag="PXH69_RS13695" /old_locus_tag="PXH69_13690" CDS complement(225..1706) /EC_number="1.-.-.-" /GO_function="GO:0016614 - oxidoreductase activity, acting on CH-OH group of donors [Evidence IEA]" /codon_start=1 /gene="mftG" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007730154.1" /locus_tag="PXH69_RS13695" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_13690" /product="mycofactocin system GMC family oxidoreductase MftG" /protein_id="WP_161120433.1" /transl_table=11 /translation="MTEADYADFLVVGGGTCGCVVAARLSEDPSAMVMLLESGSGYRSA LELPDVLGDPYRLPVGPASEYTWTYPVELTPRRASTIARGRTLGGSGAVNGAYFTRATR ADFENWPSSWRYDDVLPYFKKSETDHDFESEFHGTAGPIPVERRAWDQLHPLSGEFHAA ALGAGFPDDVDKNAPDSFGVGRVPLNVSDHRRISTAIGYLMPALHRPNLRVESGVNVIR IAFSGTRAIGIDVLDDGNVRRIRADHVVVCSGAVATPHILLNSGVGPAEQLVGQGVSVV LDRPGVGQNFVDHPEVLLPYHFSKPRTIRSQTPVLETALNLAEIEIRPYTASFTDLVPG APRMDHGVGVVLMAPRSRGSIELVSGDPAGAPRIRYNYVASAHDRAAIREGMQIAENLL ESIAETGLIDRPVVEYTDEWVESRLGTSLHMSGSCVMGAEADPFAVVDGRCRVIGAQGL SIVDTSILPTIPTRGPHATAVMVAERASAILLGDE" gene complement(1703..3100) /gene="mftF" /locus_tag="PXH69_RS13700" /old_locus_tag="PXH69_13695" CDS complement(1703..3100) /GO_process="GO:0140604 - mycofactocin biosynthetic process [Evidence IEA]" /codon_start=1 /gene="mftF" /gene_functions="biosynthetic-additional (rule-based-clusters) Glycos_transf_2" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003942091.1" /locus_tag="PXH69_RS13700" /note="Members of this protein family, MftF, are glycosyltransferases, members of PF00535 (glycosyl transferase family 2). The encoding gene is found as part of the mycofactocin cassette, in Mycobacterium tuberculosis, many other Actinobacteria, and occasional members of other lineages. Mycofactocin itself, a putative redox carrier, is a heavily modified derivative of the C-terminal Val-Tyr dipeptide of the mycofactocin precursor MftA (TIGR03969); Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_13695" /product="mycofactocin biosynthesis glycosyltransferase MftF" /protein_id="WP_007730153.1" /sec_met_domain="Glycos_transf_2 (E-value: 1.2e-24, bitscore: 81.3, seeds: 256, tool: rule-based-clusters)" /transl_table=11 /translation="MRPARLPDGFGIRLDPKVRTYSGGRVLIGGSPTRMLKLAPTAAAM IGDGFLEVVDQQSAAVARHLLDSGVANPRPMSTPSAADVTVVIPVKDNQAGVERLLPVL KDLTVIVVDDGSEVPVEPRRACPGTGTITVVRHESARGPSAARNSGLRSAQTRFVAFLD SDVIPRAGWLELMLGHFSDPGVALVAPRIVALDPYGTALARYENMRSSLDLGRKEAAVK SGSPVAYVPSAAVIVRRDVALECNGFDESLEVAEDVDFCWRLQAAGWRLRYEPVAHVAH DHRVQFDKWFARRAFYGTGAAPLAARHEGSVPPMAMSFSTLFACIAAATLTRSGLAGAL GALLFTVYRLRKMFTGLSQPTRIAAILTAQGVVGGFWQLASAMCRHYWPVTLLAVIASR RIRRLAVAAAVTEGLVDWYRHREPGGLGPVRYVFFKRADDIAYGAGLWRGAFDARDWAA LKPRITK" gene complement(3198..3890) /gene="mftE" /locus_tag="PXH69_RS13705" /old_locus_tag="PXH69_13700" CDS complement(3198..3890) /GO_process="GO:0140604 - mycofactocin biosynthetic process [Evidence IEA]" /codon_start=1 /gene="mftE" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019744248.1" /locus_tag="PXH69_RS13705" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_13700" /product="mycofactocin biosynthesis peptidyl-dipeptidase MftE" /protein_id="WP_054186896.1" /transl_table=11 /translation="MSSPRDLAAIPSPALEGTALTVVVPVGSFEQHGPHLPLDTDTRIA RAVAQALPDVVVAPAIEYGASGEHEGFAGTISIGAEAMETVVVEYGRSAFRWTKRVLFV NGHGGNGPALAKAVALLRYEGRDAAWAPCAVPGADAHAGHTETSLLLHLSPDLVHMSLA ETGNLSPIADLMPGMRSGGMVAVSQNGVLGNPVGATAEDGAYIFGELVGRVTDGLARWE PGPDGRLR" misc_feature complement(3931..3944) /note="TFBS match to ArgR, Regulator of arginine biosynthesis genes, confidence: weak, score: 17.33" /tool="antismash" gene complement(3991..5228) /locus_tag="PXH69_RS13710" /old_locus_tag="PXH69_13705" /pseudo="" CDS complement(3991..5228) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019745836.1" /locus_tag="PXH69_RS13710" /note="frameshifted; Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_13705" /product="DUF222 domain-containing protein" /pseudo="" /transl_table=11 /translation="MDSREAWQLDGVGLKSEVLDLVRARHELQSRMVLLIVEMFSREVL GGKGFRRSRSGCTGRRIWRSVSAACSSGWRGC" gene complement(5270..7219) /locus_tag="PXH69_RS13715" /old_locus_tag="PXH69_13710" CDS complement(5270..7219) /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) Pyr_redox_2" /gene_functions="biosynthetic-additional (smcogs) SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019744250.1" /locus_tag="PXH69_RS13715" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_13710" /product="mycofactocin system FadH/OYE family oxidoreductase 2" /protein_id="WP_275231837.1" /sec_met_domain="Pyr_redox_2 (E-value: 4.4e-13, bitscore: 43.3, seeds: 64, tool: rule-based-clusters)" /transl_table=11 /translation="MTPYPRLFAPLRLGNLTLRNRVVFSAHLTNYAENGLPTEQHASYY EARARGGAGLIITEEHSTHPWDRPYEKMIAGYRPEAIEGYRRITDAVHAHGSSILAQIN HNGGQGAGTYSRSPLLAPSAVADPLFREVPKAVDDADIAEILAGFALVARNCVAGGFDG VELQASQSSIVRAFLAPSTNVRTDRYGGGVAHRARFLLEVLGVLRKAIGPDRVLGVRLS GYEGTRGGIELSDAVATAQLIEADGYVDYINTTVGMATETLHLVEASMAVPTGYANFVP SAIREVVDLPVVGVGRFKTPEQAEAALEDGVCDLVGVVRGQIADPDFVNKARAGQSVQV RTCLSCNQECVGRMGLGRWLGCTENPRVGRESVPLPMPRRRGRTVVVVGGGPAGLQAAA TAAQLGHAVTLFERERRLGGQILTASVVPARRELADLVRNLESDCRRSGVTIELGAGAD LAIVEERAPEVVVIATGARPLRPAWAGGSERVVGVRDVLDGKILPSGTVLVYDELGFHQ GTSVAEFLADRGCAVTIATNGMVVAQDLSITLDFEGWNRRAFEKSIVQRTDLMVTSVSA GASPLVSLVHLPTGVTEEVAVDWVVFATQQEPVDELWNTLQGSCFEKFRIGDALSPRRS HAAVIEGHRAALAI" gene complement(7219..8046) /locus_tag="PXH69_RS13720" /old_locus_tag="PXH69_13715" CDS complement(7219..8046) /NRPS_PKS="Domain: PKS_KR (12-187). E-value: 3.5e-15. Score: 48.3. Matches aSDomain: nrpspksdomains_PXH69_RS13720_PKS_KR.1" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) adh_short" /gene_functions="biosynthetic-additional (smcogs) SMCOG1001: short-chain dehydrogenase/reductase SDR" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003941966.1" /locus_tag="PXH69_RS13720" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_13715" /product="mycofactocin-coupled SDR family oxidoreductase" /protein_id="WP_003941966.1" /sec_met_domain="adh_short (E-value: 4.3e-32, bitscore: 105.8, seeds: 230, tool: rule-based-clusters)" /transl_table=11 /translation="MTQGSVRSLEGRVAFITGAARGQGRAHAVKMAREGAAIIAVDVCA SVASDNSYDAATSEDFAETIRLVEAEGGKILAREVDVRDGAALTAVVKDGVEQFGRLDI VVANAGVCNWNRFWEMSDEQWETLIDINLTGVWKTLKASVPAIIEGGRGGSIIVVSSVA GLKALPGQAHYASAKFGLVGLTQAAAKELGEYKIRVNSIHPYGVNTPMGVDQGALEVFA KFPQYLPNFTPILSDIAFAEPDEIADTVLWLAGDGSRTVTASHIALDQGNSKV" aSDomain complement(7486..8010) /aSDomain="PKS_KR" /aSTool="nrps_pks_domains" /database="nrpspksdomains.hmm" /detection="hmmscan" /domain_id="nrpspksdomains_PXH69_RS13720_PKS_KR.1" /evalue="3.50E-15" /label="PXH69_RS13720_PKS_KR.1" /locus_tag="PXH69_RS13720" /protein_end="187" /protein_start="12" /score="48.3" /specificity="KR activity: inactive" /specificity="KR stereochemistry: C2" /tool="antismash" /translation="VAFITGAARGQGRAHAVKMAREGAAIIAVDVCASVASDNSYDAAT SEDFAETIRLVEAEGGKILAREVDVRDGAALTAVVKDGVEQFGRLDIVVANAGVCNWNR FWEMSDEQWETLIDINLTGVWKTLKASVPAIIEGGRGGSIIVVSSVAGLKALPGQAHYA SAKFGLVGLTQA" CDS_motif complement(7516..7578) /aSTool="nrps_pks_domains" /database="abmotifs" /detection="hmmscan" /domain_id="nrpspksmotif_PXH69_RS13720_0001" /evalue="1.40E-04" /label="PKSI-KR_m4" /locus_tag="PXH69_RS13720" /protein_end="177" /protein_start="156" /score="14.4" /tool="antismash" /translation="IVVSSVAGLKALPGQAHYASA" gene complement(8043..9974) /locus_tag="PXH69_RS13725" /old_locus_tag="PXH69_13720" CDS complement(8043..9974) /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) Pyr_redox_2" /gene_functions="biosynthetic-additional (smcogs) SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007728304.1" /locus_tag="PXH69_RS13725" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_13720" /product="mycofactocin system FadH/OYE family oxidoreductase 1" /protein_id="WP_275231838.1" /sec_met_domain="Pyr_redox_2 (E-value: 1.6e-13, bitscore: 44.7, seeds: 64, tool: rule-based-clusters)" /transl_table=11 /translation="MTLDEHTALAGRLVRSRVVFGPHETNLAVGRSLSDRHRAYYQRRA EGGTGIVVTEVASVSENDWPYERAPLASQCGPGWQSIAAACAPYGTLVLAGLGHTGMQG SSAYSQSVLWGPSRVADPATRELPMAMGQPEIDQLIVGFREASALAVASGMDGVELDAG PRSILRQFLSGLTNTRQDRYGSDRVALVREVISAVREEVGSDHVVSLRLSCDEEAPWAG LTPDLAIGIAREVSPFLDLLVVVRGGLYSSDRYRPDFHCPPNFNLDLCREIKVALSIPV VVQGSVVDIADAHAALEVGEFVEMTRAQIADPDLVVKASRGDRPRPCVLCNQTCLVRDP RNPVVTCVGNPSAGFETVDPDEREQISTPGAVLVVGGGPAGMEAARISAVAGHSVTLVE SLSQFGGMIRTFARGDGRSRFALLAQWLEDECRRSGVTMRTGAALDAQDLGDARSRGEF VVVATGSAARPPTYPVDDSVRWLDASEAFEKLSTVGPGPVALIDPLGGPIAVALAEALA AHGISVSIVTPDSIVGSRLGASGDLVGANTRIQQAGITRVLASRVTEIARGAVCVEHVH TGERSQLECEVVIDCSPRLPNETLKEEADIQVGDSVAPRTVLESIREGRFAGFTTRTSF NSAERAIS" gene complement(10001..11179) /gene="mftD" /locus_tag="PXH69_RS13730" /old_locus_tag="PXH69_13725" CDS complement(10001..11179) /EC_number="1.4.3.26" /GO_process="GO:0140604 - mycofactocin biosynthetic process [Evidence IEA]" /codon_start=1 /gene="mftD" /gene_functions="biosynthetic (rule-based-clusters) redox-cofactor: TIGR03966" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003942047.1" /locus_tag="PXH69_RS13730" /note="MftD, an enzyme found in the mycofactocin biosynthesis locus, performs an oxidative deamination of 3-amino-5-[(p-hydroxyphenyl)methyl]-4, 4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound, now called pre-mycofactocin (PMFT), is a biologically active redox cofactor that can oxidize the non-exchangeable NADH of TIGR03971 family SDR-type oxidoreductases.; MftD, an enzyme found in the mycofactocin biosynthesis locus, performs an oxidative deamination of 3-amino-5-[(p-hydroxyphenyl)methyl]-4, 4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound, now called pre-mycofactocin (PMFT), is a biologically active redox cofactor that can oxidize the non-exchangeable NADH of TIGR03971 family SDR-type oxidoreductases.; MftD, an enzyme found in the mycofactocin biosynthesis locus, performs an oxidative deamination of 3-amino-5-[(p-hydroxyphenyl)methyl]-4, 4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound, now called pre-mycofactocin (PMFT), is a biologically active redox cofactor that can oxidize the non-exchangeable NADH of TIGR03971 family SDR-type oxidoreductases.; MftD, an enzyme found in the mycofactocin biosynthesis locus, performs an oxidative deamination of 3-amino-5-[(p-hydroxyphenyl)methyl]-4, 4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound, now called pre-mycofactocin (PMFT), is a biologically active redox cofactor that can oxidize the non-exchangeable NADH of TIGR03971 family SDR-type oxidoreductases; Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_13725" /product="pre-mycofactocin synthase MftD" /protein_id="WP_003942047.1" /sec_met_domain="TIGR03966 (E-value: 3.5e-221, bitscore: 727.8, seeds: 10, tool: rule-based-clusters)" /transl_table=11 /translation="MAKNAFFETVAEAQRRAKKRLPKSVYAALVAGSEKGLTVDDNVAA FSELGFAPHAAGLSDKREMSTTIMGQDISLPVMISPTGVQAVHPDGEVAVARAAAARGT AIGLSSFASKSIEEVAAANPQVFFQMYWVGSRDVLLQRMERARAAGAKGLIITTDWSFS YGRDWGSPSIPEKMDLKAMFQFAPEGIMRPKWLLEFAKTGKIPDLTTPNLAAPGQPAPT FFGAYGEWMQTPLPTWEDIAWLREQWGGPFMLKGIMRIDDAKRAVDAGVSAISVSNHGG NNLDGTPAPIRVLPGIAEAVGDQVEVVLDGGIRRGGDVVKALALGAKAVMLGRAYLWGL SANGQAGVENVLDLMRMGIDSGLMGLGHSSITELSPADLVIPEGFTRTLGAS" gene complement(11235..12452) /gene="mftC" /locus_tag="PXH69_RS13735" /old_locus_tag="PXH69_13730" CDS complement(11235..12452) /EC_number="1.3.98.7" /EC_number="4.1.99.26" /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding [Evidence IEA]" /GO_process="GO:0031179 - peptide modification [Evidence IEA]" /GO_process="GO:0140604 - mycofactocin biosynthetic process [Evidence IEA]" /codon_start=1 /gene="mftC" /gene_functions="biosynthetic (rule-based-clusters) redox-cofactor: PF04055" /gene_functions="biosynthetic (rule-based-clusters) redox-cofactor: SPASM" /gene_functions="biosynthetic (rule-based-clusters) redox-cofactor: TIGR04085" /gene_functions="biosynthetic-additional (rule-based-clusters) Fer4_12" /gene_functions="biosynthetic-additional (rule-based-clusters) QueE" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_006930977.1" /locus_tag="PXH69_RS13735" /note="MftC is a radical SAM/SPASM enzyme that catalyzes the first two steps in biosynthesis of the electron carrier mycofactocin from the terminal Val-Tyr dipeptide of the precursor peptide MftA.; MftC is a radical SAM/SPASM enzyme that catalyzes the first two steps in biosynthesis of the electron carrier mycofactocin from the terminal Val-Tyr dipeptide of the precursor peptide MftA; Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_13730" /product="mycofactocin radical SAM maturase" /protein_id="WP_003942089.1" /sec_met_domain="PF04055 (E-value: 3.1e-24, bitscore: 80.4, seeds: 518, tool: rule-based-clusters)" /sec_met_domain="Fer4_12 (E-value: 1.4e-07, bitscore: 26.1, seeds: 84, tool: rule-based-clusters)" /sec_met_domain="QueE (E-value: 4e-11, bitscore: 37.0, seeds: 32, tool: rule-based-clusters)" /sec_met_domain="TIGR04085 (E-value: 8e-16, bitscore: 52.5, seeds: 281, tool: rule-based-clusters)" /sec_met_domain="SPASM (E-value: 5e-07, bitscore: 24.4, seeds: 166, tool: rule-based-clusters)" /transl_table=11 /translation="MTSVLEQPSPPAKVGRLVDQFELGLDAPICLTWELTYACNLSCVH CLSSSGRRDPNELSTEQCKSIIDELQRMQVFYVNIGGGEPTVRSDFWELVDYATDHQVG VKFSTNGVKIDKKVAARLAASDYVDVQISIDGATAEVNDAVRGPGSFAMAERALANLKE AGFKDAKISVVVTRHNVSQLDEFKALADKYDATLRITRLRPSGRGADVWDDLHPRPEQQ RELYNWLVANGEGVLTGDSFFHLSAFGDALPGLNLCGAGRVVCLIDPVGDVYACPFAIH EQFLAGNIVKDGGFQEVWQHSDLFEELRSPQTGGACSKCNHYDSCRGGCMAAKFFTGLP MDGPDPECVIGNGEMALASAGEVPKSSVDHSRSGQRKTPRASVPLTLMMRPPAKICDEN PLAGMQ" gene complement(12529..12816) /gene="mftB" /locus_tag="PXH69_RS13740" /old_locus_tag="PXH69_13735" CDS complement(12529..12816) /GO_process="GO:0140604 - mycofactocin biosynthetic process [Evidence IEA]" /codon_start=1 /gene="mftB" /gene_functions="biosynthetic (rule-based-clusters) redox-cofactor: Mycofactocin_RRE" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007728309.1" /locus_tag="PXH69_RS13740" /note="MftB, a small protein, is a peptide chaperone that assists the radical SAM enzyme MftC in performing two modifications to the C-terminal Val-Tyr dipeptide of the mycofactocin precursor peptide, MftA. MftB's role is analogous to the role of PqqD in the biosynthesis of PQQ, a cofactor that derives entirely from a Tyr and a Glu in the precursor PqqA.; MftB, a small protein, is a peptide chaperone that assists the radical SAM enzyme MftC in performing two modifications to the C-terminal Val-Tyr dipeptide of the mycofactocin precursor peptide, MftA. MftB's role is analogous to the role of PqqD in the biosynthesis of PQQ, a cofactor that derives entirely from a Tyr and a Glu in the precursor PqqA; Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_13735" /product="mycofactocin biosynthesis chaperone MftB" /protein_id="WP_007728309.1" /sec_met_domain="Mycofactocin_RRE (E-value: 1.7e-31, bitscore: 102.5, seeds: 14, tool: rule-based-clusters)" /transl_table=11 /translation="MRRVLTVSALDLDAGWKLHPQVALRPEPFGALLYHFGTRKLSFLK NTTIVAVVKALPDHPDVRSALRAQGIEDDAAPQYARALSTLADSHMIVPV" aSDomain complement(12541..12780) /aSDomain="Mycofactocin_RRE" /aSTool="RREfinder" /database="RREFam.hmm" /description="Stand-alone RRE protein in a mycofactocin cluster" /detection="hmmscan" /domain_id="rrefinder_PXH69_RS13740_Mycofactocin_RRE.1" /evalue="1.60E-32" /identifier="RREFam016.1" /label="PXH69_RS13740" /locus_tag="PXH69_RS13740" /protein_end="92" /protein_start="12" /score="102.5" /tool="antismash" /translation="DAGWKLHPQVALRPEPFGALLYHFGTRKLSFLKNTTIVAVVKALP DHPDVRSALRAQGIEDDAAPQYARALSTLADSHMI" gene complement(12801..12896) /gene="mftA" /locus_tag="PXH69_RS13745" /old_locus_tag="PXH69_13740" CDS complement(12801..12896) /GO_function="GO:0009055 - electron transfer activity [Evidence IEA]" /GO_process="GO:0140604 - mycofactocin biosynthetic process [Evidence IEA]" /codon_start=1 /gene="mftA" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_005573048.1" /locus_tag="PXH69_RS13745" /note="Mycofactocin is a small molecule electron carrier derived from the final two amino acids, Val-Tyr, of MftA, the mycofactocin precursor. It plays a role in redox homeostasis and the metabolism of alcohols and aldehydes in Actinobacteria, including Mycobacterium tuberculosis; Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_13740" /product="mycofactocin precursor MftA" /protein_id="WP_063836238.1" /transl_table=11 /translation="MSDRDNKALENDLVEESLVEEVSIDGMCGVY" misc_feature 12880..12896 /note="TFBS match to ZuR_variant_2, Zinc-responsive repressor, confidence: weak, score: 18.73" /tool="antismash" misc_feature complement(12973..12988) /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2, activator of antibiotic production, confidence: medium, score: 17.11" /tool="antismash" misc_feature complement(12983..12998) /note="TFBS match to CRP, Cyclic AMP receptor protein, confidence: medium, score: 17.13" /tool="antismash" gene 13047..13646 /gene="mftR" /locus_tag="PXH69_RS13750" /old_locus_tag="PXH69_13745" CDS 13047..13646 /GO_function="GO:0003700 - DNA-binding transcription factor activity [Evidence IEA]" /GO_function="GO:0036042 - long-chain fatty acyl-CoA binding [Evidence IEA]" /GO_process="GO:0006355 - regulation of DNA-templated transcription [Evidence IEA]" /codon_start=1 /gene="mftR" /gene_functions="regulatory (smcogs) SMCOG1215: TetR family transcriptional regulator" /gene_kind="regulatory" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007728311.1" /locus_tag="PXH69_RS13750" /note="MftR, the mycofactocin system transcriptional regulator, is an uncharacterized TetR family DNA-binding transcription factor. Its role is inferred by context. It occurs as part of the biosynthesis locus for mycofactocin, a partially characterized electron carrier derived from the terminal Val-Tyr dipeptide of the precursor peptide MftA, through a radical SAM enzyme-mediated process; Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_13745" /product="mycofactocin system transcriptional regulator" /protein_id="WP_007728311.1" /transl_table=11 /translation="MRSRKKPSARIGRRPSTTKEGISTIGIELFSTVGFNEVSVDQIAD AAGIARRTFFRYFPSKNAVPWGDFDGHLDEMREHLDSLPADIPLVDALESALLTFNTFP PEETAVHRKRMELILEVPTLQAYSVVMYEGWRQVVADYVARRMNLEPTDHGPRTVGYLL LGVAMSAYEQWLTDDSLELPELLKTGTQSLRTGIST" gene 13676..14509 /gene="mftM" /locus_tag="PXH69_RS13755" /old_locus_tag="PXH69_13750" CDS 13676..14509 /codon_start=1 /gene="mftM" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007728312.1" /locus_tag="PXH69_RS13755" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_13750" /product="mycofactocin oligosaccharide methyltransferase MftM" /protein_id="WP_007728312.1" /transl_table=11 /translation="MSTITNEPVLDSLAPSTPGLWSFGHVTVERVESGPISLDRRGGRL HVRHSLTPADLSERLVAGVTSSIQDAEFGQDEFELTMVGLVRSSVDGALESWTTYYRNS LAELLDGTADFAPIHDKAEELVRGSVLDLGSCFGFLPLRLARAGMDVTATDILPGTMIL LDAVAPELGITIDTVICDAGEVPAPDNSADTVTAIHLLEHVDDAVGEAVIAQALRIARQ RVVIAVPFEDEATACHGHIRTFDLESLRAVGRQSRVPFEVFEHHGGWLVLDASAA" gene complement(14516..14719) /locus_tag="PXH69_RS13760" /old_locus_tag="PXH69_13755" CDS complement(14516..14719) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003942010.1" /locus_tag="PXH69_RS13760" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_13755" /product="DUF4287 domain-containing protein" /protein_id="WP_007728313.1" /transl_table=11 /translation="MTEPVKGPASYFPSIEKKYGRPIGEWQELIRSSDLTKHMELVNWL KSEHGMGHGHANALVAHTLKEG" gene 14861..15073 /locus_tag="PXH69_RS13765" /old_locus_tag="PXH69_13760" CDS 14861..15073 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_005058188.1" /locus_tag="PXH69_RS13765" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_13760" /product="TOBE domain-containing protein" /protein_id="WP_003942061.1" /transl_table=11 /translation="MRLSTRNQLTGTITEVNLGTVMATVKVKLDGGNQIVTSSITRDAA LDLDLEPGQAATVFVKSTEVTIGVE" gene complement(15090..15515) /locus_tag="PXH69_RS13770" /old_locus_tag="PXH69_13765" CDS complement(15090..15515) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019744256.1" /locus_tag="PXH69_RS13770" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_13765" /product="DUF6314 family protein" /protein_id="WP_007728314.1" /transl_table=11 /translation="MVDDAKIEPRALVGKWHFERDIVDRLSGSTKHVFGETTISDAGDG RMRWYESGTLRDGDLELPVFRTLFVAERGLEWAVTFEDGREFHDWAPGEEVEHLCGADT YRGRIDVTAGDRSTWSVVWTVTGPSKDYTMTTHLTRP" gene 15626..16102 /locus_tag="PXH69_RS13775" /old_locus_tag="PXH69_13770" CDS 15626..16102 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007728315.1" /locus_tag="PXH69_RS13775" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_13770" /product="MaoC/PaaZ C-terminal domain-containing protein" /protein_id="WP_007728315.1" /transl_table=11 /translation="MNDSAALHKRAVFAEDLEIGQVIPLGSYSVTEEEIVEFATQWDPQ WFHVDKEAAESGVFGGLIASGLHTLSICQKLVVASLYDQWNVIAGKTMRDVSFLRPLRP GDTLSGKVTVDNVVFDNRARGLVTITAKLVDAHGNRVLDVVTDAYLHCRSTRGQ" gene 16336..17124 /locus_tag="PXH69_RS13780" /old_locus_tag="PXH69_13775" CDS 16336..17124 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003941978.1" /locus_tag="PXH69_RS13780" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_13775" /product="thioesterase family protein" /protein_id="WP_065352149.1" /transl_table=11 /translation="MAITSQVSVAAAPSTDAARHHTEFDSSWRSFDGIQGGLAIARMLR VAADASGAEPLAVTAHLDRPVPPGPAELTVGEMRGGRTVSCHVTLTGSGSALVRLARDI GATTMSAPVAEPAPADPSTLPPLEIPVDFVPFAQHLDIRPINSARPFAGGDSPEFDVWI RLLTDLDFTAQERAAVLLDALPPGLFATLTMPVAIPTIEFTAHFTPEHLTADPRTLSPW YRLRHGTAWATDTLCVDESELFDSTGRLAARARQLRRILS" gene complement(17137..18807) /locus_tag="PXH69_RS13785" /old_locus_tag="PXH69_13780" CDS complement(17137..18807) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003941963.1" /locus_tag="PXH69_RS13785" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_13780" /product="hypothetical protein" /protein_id="WP_007728318.1" /transl_table=11 /translation="MTVVVEAAPPVPASASQFTGTRRLVRLALRRDRIQLSVWVISIVL FMLLVVASVTGLYSTPEELRNIAVSSAVSPVALATNGIVSGDSQGAIVASQSVLVFALV AALMSTLAVVRHTRQNEETGRAEMIGAAVVGRRALLTSALIVTVGANVVLALGIALGSI AVGLPVVGSFALGASIGGTGIAFAGVAAVTAQITDGARTANGLAGAAIGVAFMLRAIGD VNSTVVDDGTRVVSGWLSWLSPIGWAQQIRPFDDDAWWILLLLLGFAAVHVVVAFVLIG HRDQGAGLVQPRPGPPVAASWLPSAWGLAWRMQRMTMFWWIFAVVILGFAYGAVGNEID AMVGSSQQTAEMFEKLGGGGSDLVDSYLATALGLTSIVIAAYSVQSLLRMRGEETSGRL EPLLATSLSRWRWMLSHLGVVVGGVILAHVLMGAFTGFAFGLVTDDIGGNTLRMTGAAL AYLPAVLVVVGLVSLAFGLFPTYASALSWGILAACIVLGQLGTLLKLPQVVLDLSPFTH VPGAPAEPVTVLPLLAMIVVAVVLSTASLVAFRARDLQV" gene complement(18804..19739) /locus_tag="PXH69_RS13790" /old_locus_tag="PXH69_13785" CDS complement(18804..19739) /GO_function="GO:0005524 - ATP binding [Evidence IEA]" /codon_start=1 /gene_functions="transport (smcogs) SMCOG1000: ABC transporter ATP-binding protein" /gene_kind="transport" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019744261.1" /locus_tag="PXH69_RS13790" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_13785" /product="ABC transporter ATP-binding protein" /protein_id="WP_007728320.1" /transl_table=11 /translation="MGAAISVKSLVKTFGTTRALDGLDLDVSTGEVHGFLGPNGSGKST TIRVLLGLLRSDSGDATVLGGDPWRDAVTLHRRLAYVPGDVNLWPNLTGGEAIDLLAGL RGGLDRKRRSELLDRFELDPTKKARTYSKGNRQKVALVAAFSSDVDLYVLDEPTSGLDP LMEAVFQDCVEEVAHAGKTVLLSSHILAEVEALCDRVSIIRDGRTVEEGTLSELRHLTR TSVAAETQLPIGGLDGLGGVHDAVIDGNHVTCEVDSAQLGPLMTYLVGFGIVALTSTPP TLEELFLRHYGDERARETSVHLRAETGVRP" gene 19808..20302 /locus_tag="PXH69_RS13795" /old_locus_tag="PXH69_13790" CDS 19808..20302 /GO_function="GO:0003677 - DNA binding [Evidence IEA]" /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007728322.1" /locus_tag="PXH69_RS13795" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_13790" /product="helix-turn-helix domain-containing protein" /protein_id="WP_007728322.1" /transl_table=11 /translation="MTDTSHTVAPDESRHYVERLSLVLSDMGFPPMPARVWAALMTSET PSSTPGDLGSRLGVSPAAISGAVRYLQQVGLIERVAVPGSRRQHYCVSVDMWADAFVTK QSSLYQVSAVADEGIRVLGEGTTAGDRMADLRDFFDFMAAEMPRMLADWRSRRKDRTPG R" misc_feature 19854..19872 /note="TFBS match to FuR_variant_2, Ferric Uptake Regulator, confidence: weak, score: 21.62" /tool="antismash" gene 20386..20925 /locus_tag="PXH69_RS13800" /old_locus_tag="PXH69_13795" CDS 20386..20925 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003941864.1" /locus_tag="PXH69_RS13800" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_13795" /product="hypothetical protein" /protein_id="WP_007728324.1" /transl_table=11 /translation="MKLSIRGANTSPTPPFARTASPETCERSISLNRTAAEAFAGDHHA LSKLLARLHPRVVLYYRARLLGTPVATFADDLAHQCTLDVVTRMSDTISADTRIETFAY ELASRRIASIPQSRNIDGQFKRPRRLTDDQREVLVLRIAVGLTAEMTASTLRTTRSSVL LLQHQALRAWTVSEQS" gene complement(20922..21605) /locus_tag="PXH69_RS13805" /old_locus_tag="PXH69_13800" CDS complement(20922..21605) /GO_function="GO:0003677 - DNA binding [Evidence IEA]" /GO_process="GO:0000160 - phosphorelay signal transduction system [Evidence IEA]" /GO_process="GO:0006355 - regulation of DNA-templated transcription [Evidence IEA]" /codon_start=1 /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family DNA-binding response regulator" /gene_kind="regulatory" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019744264.1" /locus_tag="PXH69_RS13805" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="PXH69_13800" /product="response regulator transcription factor" /protein_id="WP_003942005.1" /transl_table=11 /translation="MTTARPVITRGASPVQGLVRIVLVDDHAILRQGLRSVLERESDLE IVGEASSLAEALAVVDKVGPDIVLMDLKLSASSDYEGLTLCGQLSSAHPGLGLLVLTTF LDDQLVVRAVHAGARGYVVKDVDTTELVRAIRAVSRGESAFDSRSASAVVRSLNGQSSS KERLTDRELEVLRLLANGLSNIKIGERLFISPTTVKFHVSNIMRKLEVSRRAEAVYAAS KQGLI" misc_feature 21635..21646 /note="TFBS match to CcpA, Catabolite control protein A, confidence: medium, score: 17.0" /tool="antismash" misc_feature complement(21803..21821) /note="TFBS match to FuR_variant_2, Ferric Uptake Regulator, confidence: weak, score: 22.94" /tool="antismash" ORIGIN 1 ccgcgggcat cgatgaacga ctgatgggca gacgaaagtc gccgcgcgag taacgcgacg 61 tcatcgcctg gcctgaccgc cacccacggg ttgccgggca cagcctgttc acgtgccata 121 ttttgcgttc ctccgctccg tggaccactg taacgccgat cacagcccat aggcgagaac 181 gaccattcgg tatgactgcc gacccagcct gctgcccctc gcggtcactc gtcaccgagc 241 agaatggcgc ttgctcgttc cgcgaccatc accgcggtcg catgcgggcc gcgggtcggg 301 atcgtcggca ggattgacgt gtcaacaata ctgagtcctt gcgcgccgat cacacggcac 361 cgaccgtcga cgactgcgaa cgggtccgcc tcggcaccca tcacgcaact accggacata 421 tgcagcgacg ttcccaggcg ggattcgacc cactcatcgg tgtactccac gacggggcga 481 tcgatcaacc ccgtctcggc gatcgactcg agaagattct cggcgatctg catcccttcc 541 cgaatggcag ctcgatcgtg cgccgacgcg acatagttgt accgaatacg cggcgcccca 601 gcgggatccc ccgataccag ttcgatgctt ccccggctgc gcggcgccat caggaccact 661 ccgacgccgt ggtccatgcg aggcgcaccg ggaaccaggt ctgtgaaaga tgcggtgtac 721 ggacggattt cgatctcagc gaggttgagg gctgtctcca gcaccggagt ctgcgatcga 781 atcgtgcggg gcttcgaaaa atggtacggc agcagcactt ccgggtggtc gacgaagttc 841 tgtccgacgc ccggtcgatc gagaacgacg gaaactccct gccctacaag ttgttccgct 901 ggaccaacac cggaattgag gagaatgtgc ggtgtcgcca ccgcgcccga acacacgacc 961 acatggtcgg cacgaatgcg tcggacattg ccgtcgtcga gaacgtcgat gccgatcgca 1021 cgggtacccg agaacgcgat acgaatgacg ttcactccgg attccacccg cagattcggc 1081 cggtgcaacg ccggcatcag atacccgatc gccgtactga tccgacgatg atccgacacg 1141 ttcagtggca ctcggcccac cccgaacgag tccggcgcat tcttgtcgac gtcgtcggga 1201 aagcctgctc cgagcgctgc cgcatgaaac tcgccgctca gtggatgcag ttggtcccac 1261 gctcgacgct cgaccgggat cggtccggcg gtcccgtgga actcgctctc gaaatcatga 1321 tccgtttcgg acttcttgaa gtacggcaag acgtcgtcgt atcgccatga actcggccaa 1381 ttctcgaaat cagcacgggt cgcgcgggtg aagtacgcac cgttgacggc tccggatcca 1441 ccgagtgtcc gcccccgcgc aattgtggac gcccgacgcg gcgtcagttc gaccggatag 1501 gtccacgtgt attcgctcgc aggtccgacc ggcagtcggt acggatcacc caggacgtcc 1561 ggaagttcga gcgccgatcg atatccgctt cccgattcga ggagcatcac catggcggat 1621 ggatcttcac tgagcctggc tgcgaccacg caaccacacg tgccaccacc gacaaccagg 1681 aaatctgcgt agtcggcttc tgtcacttgg tgattcgagg tttcagcgcc gcccagtcgc 1741 gggcgtcgaa tgcgccacgc cagagtccgg ctccatacgc gatgtcgtcg gctcgtttga 1801 agaagacgta ccgaaccggt cccagcccgc ctggctcacg gtggcgatac cagtccacca 1861 aaccttcggt gactgctgca gcgacggcca gccgccgaat tcgacgggag gcgatcaccg 1921 ccaggagcgt cacgggccag tagtgacgac acattgccga ggcgagttgc cagaatccgc 1981 cgacaactcc ctgcgcggtg aggatcgccg cgatccgggt gggttgactc aggccggtga 2041 acatctttcg cagccggtaa acggtaaaca gcaaagcgcc gagtgcgccg gcaagaccgg 2101 aacgggtcag tgttgcagcg gcgatacacg cgaagagcgt cgagaacgac atcgccatcg 2161 gcggaaccga accctcgtgt cttgcagcca acggcgccgc acccgtgccg tagaacgctc 2221 tcctggcgaa ccacttgtcg aactgaactc gatgatcgtg ggcgacatgc gcaaccggtt 2281 cgtacctcag tcgccaaccc gcagcctgca gtcgccagca gaaatcgacg tcctcggcta 2341 cttcgagact ctcgtcgaaa ccgttgcatt cgagtgccac gtcgcgccgg acgatcacgg 2401 cggcgctggg gacataggcg accggactgc ccgacttcac cgcagcttcc ttgcgtccga 2461 ggtcgagaga cgatcgcatg ttctcgtacc gcgccagcgc cgtcccgtac ggatcgaggg 2521 cgacgatgcg gggtgcaaca agggcaaccc ccggatcgct gaagtggccc aacatcaact 2581 cgagccaacc ggcacgcggt atgacgtcgg agtccagaaa agctacgaaa cgtgtttggg 2641 ccgaacgcaa tcccgagttt ctcgctgccg agggtccgcg ggcactctcg tgccggacca 2701 ctgtgatggt gccggtgccc gggcaggcgc gacggggctc caccggcact tccgaaccgt 2761 cgtcgacaac gatgacggtc agatccttga ggacgggcaa taggcgttcg acgccggcct 2821 ggttgtcctt gaccggaatc acgaccgtga catcggcagc ggacggcgtc gacatcggac 2881 gaggattcgc gacacctgaa tcgagaaggt gtcgcgcaac cgcagcgctc tgctgatcga 2941 ccacctcgag gaagccgtca ccgatcatcg ccgcagccgt cggcgccagc ttgagcatcc 3001 gtgtcggaga tccgccgatg agtacccggc cacccgagta cgtgcgcacc ttggggtcca 3061 accgaatgcc gaagccgtcg gggagtcttg ctggtcgcat cagatgatcg taagggacgt 3121 agcccgtcgt gctgcaactc gtcgcagcct cgaaagccgt cgcaggtgga ccgtccgccc 3181 ccactcggtc gtggtcgtca ccgaagcctc ccatctggac caggctccca ccgggccagt 3241 ccatccgtca cgcgtcctac caactcaccg aagatgtatg cgccgtcttc ggcggtagcc 3301 ccgactggat tgccgagaac gccgttttgc gacacagcga ccataccgcc cgaacgcatt 3361 cctggcatca ggtccgcgat aggagacaaa tttcctgtct cagcaaggct catgtgcacc 3421 aaatcgggcg aaaggtgcag caataggctc gtttcagtgt gtccggcatg cgcgtcggca 3481 cccggaacag cgcacggcgc ccaagcagca tcacgcccct cataccgcag aagtgccacc 3541 gctttggcca atgcgggacc atttccgccg tggccgttga cgaacagcac ccgcttcgtc 3601 catcggaacg ccgaccttcc gtattcgacg acgacggttt ccatggcctc ggcaccgatc 3661 gaaatcgtgc cggcaaaccc ttcgtgttcg ccgctcgccc cgtattcgat ggccggggcc 3721 acgacgacgt cgggcagagc ctgtgcgact gctctcgcga tccgagtgtc ggtatcgaga 3781 ggcaggtgcg gtccgtgttg ttcgaaagaa ccgaccggga ccacgacggt gagcgcagtg 3841 ccctcgagcg caggactcgg gatggcagca aggtcacgcg gagaactcat cccccgatcg 3901 tattggtgtg ggcccctccc accgcaacac tgcatgcgga tacaccgggg cattttgttg 3961 tgccccggcg tattcgtcag ttgtcgcggt tcacgccgct gtcggtgggc tgttcccgcc 4021 gatcggctgt tgatacggat cgatcgacat cggtggatag aacaccgatt tgcgatccgg 4081 cccgatctcg acccgccatt cggtgtggtg cagtgtccga tgatgatgac cgcacaacaa 4141 gatcagattg tcgagatctg ttgggccacc atcgttccag tgaacaatat gatgggcatc 4201 gcaccacccc gacggtgtcc cgcaccccgg aaacgcacac ccaccatccc gaaccgcaag 4261 agcccggcgc tgcggaaccg tcgccgttct cgcttccttg ccgcagtcga gcggaacacc 4321 attcgcatcc aacaagatcc gcgtcacggt gcagtcgcac gccaacatcc gcgcagtgtc 4381 gatactgatc gggcccaccc aatcggtcca cgcaaatccg agttcttcgg tggacggcag 4441 gaggtctttc aacgccttga ggtcggtcat gtctttcgcg gacgcggtaa cggtgatgtg 4501 tggtttcact ccgccttcga tcggacccaa ccccgcccgc tcgtaccggc gcagtagttc 4561 gcagaacccg tccgcacgtc gtaatgccgg agtgcgggtg tctttggcgc cgtctttctc 4621 cggcgtcgga gccgacagac gcgacaacaa tgtgatcaga cgcgccccgt tcacagcatc 4681 taggtctccc ttcaccgaca cgcgcccgta caaacctttc gacgcgtaga actcgttacg 4741 ctccgaatct tcccccaccg gaacgccgtc ctcacggttt ccgtactgct tgtcgatccg 4801 ccggatcgcc gcccgcaccg catcacaatc agcaatattc tgcgacgcca ggccaagcag 4861 gatcccgcga gccttgtcca catccgccag ggtcatgttc ttcggtggat gctgacaaaa 4921 cgacgccacc tgccgcgcct tcaacgcatc aacgtcaccg cgatggaatg catccgcgac 4981 aagaggttcg agcatcaaca accgcgccaa cccgacgagc aggctgcact caccgatctc 5041 cagattcgtc gacccgtgca accactgcgc gatcgccgaa aacccttccc gccgagaacc 5101 tcacgggaaa acatctccac gatcagcaac accatccgcg actgcaattc gtgtcgggcg 5161 cgcaccagat cgagaacctc actcttgagg cccacgccat ccagttgcca cgcttcccga 5221 ctgtccatgc tcaagacata ctttcgagaa ccagggacaa aacggacgac tatatcgcca 5281 acgcggcccg atgcccctcg atgacggccg cgtgcgaacg cctcggagac aaggcgtcac 5341 cgatgcgaaa tttctcgaaa cagcttccct gcaaggtgtt ccacaactcg tcgacaggtt 5401 cctgctgtgt cgcgaagacc acccagtcga ctgcaacttc ctcggtcaca cccgtcggaa 5461 ggtgaaccaa gctcacgagc ggcgaggccc cagcactcac cgaagtcacc atcagatctg 5521 tccgttgcac gatcgacttc tcgaacgccc ggcggttcca cccctcgaaa tccagggtga 5581 tgctcaggtc ctgcgcgacc accatgccgt tggtcgcgat cgtgacggca cagcctcgat 5641 cagcgaggaa ctcagccacc gacgtacctt gatggaatcc cagttcgtcg tagacgagca 5701 ccgttccact cggcagaatc ttgccgtcga gaacgtctcg aacacccacc acacgctctg 5761 atccacccgc ccacgctggg cgtaaaggcc gcgctccggt tgcaatcacg accacctcgg 5821 gcgcgcgctc ctccacgatc gcgagatcag ctccagcgcc cagctcgatc gtcacaccgc 5881 ttcggcgaca atcagattcg agattccgga cgaggtcggc gagctcacgg cgagccggta 5941 ccaccgaggc ggtaagaatc tgtcccccaa gtcttcgttc acgctcgaac agcgtcaccg 6001 catgacctag ctgggccgct gtagccgcag cttgcaggcc ggcggggccg ccaccgacca 6061 ccaccaccgt gcggccccgt cgccgcggca tcggcagcgg taccgattcc cgaccgacgc 6121 gcggattttc ggtgcacccc aaccaacgtc ccagacccat ccgcccgacg cattcctggt 6181 tgcaggacag gcaggttcgc acctgtaccg attgaccagc acgcgccttg ttcacaaaat 6241 ccggatcggc gatctgcccg cgaactactc ccaccagatc gcatacaccg tcctcgagtg 6301 cggcttcggc ttgctccggc gtcttgaaac ggccgactcc gacgactgga agatcgacga 6361 cctccctgat cgcagacggg acgaagttgg cgtagccggt gggtacagcc atcgatgctt 6421 cgaccagatg cagtgtttcc gtggccattc cgacggtggt gttgatgtag tcgacgtaac 6481 cgtcagcttc gatgagttga gcggtggcta ctgcgtcgga cagttcgatg ccaccccgcg 6541 ttccttcgta tccactcagg cgcacaccga ggacacgatc cgggccgatg gccttacgca 6601 gtaccccgag cacttcgaga aggaaccgtg cacgatgtgc cacgcccccg ccgtagcgat 6661 cggtgcgcac gttggtcgag ggggcgagaa acgcgcgcac tatggacgac tgcgacgcct 6721 gcaattccac cccgtcgaag ccaccggcga cgcaattgcg ggcaacaagg gcgaaccctg 6781 cgagaatctc ggcgatgtcc gcgtcgtcga cagctttggg cacctcgcgg aacaacggat 6841 cggcgacggc gctcggcgcc aacagcggcg atcgcgaata ggttccagcg ccctggcccc 6901 cattgtggtt gatctgcgcg agaatcgacg aaccatgtgc atgaacggcg tcggtgatcc 6961 gccggtatcc ctcgatcgct tcgggccgat agcccgcaat catcttctcg tacggtcgat 7021 cccaaggatg cgtcgagtgt tcctcggtga tgatcagacc cgcaccgccg cgtgcccgcg 7081 cctcgtaata gctcgcgtgc tgctccgtcg gcaacccgtt ctccgcgtaa ttggtcaggt 7141 gtgcggagaa caccacccga ttgcggagtg tcaggttgcc caggcgcagt ggcgcgaaga 7201 ggcgcgggta cggcgtcatc agaccttgga gtttccttga tcgagcgcga tatgacttgc 7261 cgtgacggtg cgggaaccgt caccggcgag ccacaggacg gtatcggcga tctcgtcagg 7321 ttcggcgaaa gcgatgtccg agaggatcgg agtgaagttg ggaaggtact gcggaaactt 7381 tgcaaatacc tcgagagctc cctggtcgac ccccatcggc gtattcactc cgtaaggatg 7441 gatcgaattg accctgatct tgtactcccc cagttccttt gctgcggcct gcgtcagacc 7501 gacgagaccg aacttggcgc tcgcgtagtg cgcctgtccg ggcagcgcct tgagtccggc 7561 caccgaactg accacgatga tcgatccacc gcgcccaccc tcgatgatcg ccggcaccga 7621 ggccttgagg gttttccaga ctccggtcag gttgatgtcg atcagagttt cccactgctc 7681 gtccgacatt tcccagaatc gattccagtt gcagaccccg gcgttggcga ccacgatgtc 7741 gagtcgtccg aactgttcga cgccgtcctt gaccaccgcg gtgagcgctg cgccgtcacg 7801 gacatcgacc tcgcgggcga ggatcttccc gccctccgct tccaccagac gtatcgtctc 7861 ggcaaagtcc tcggaagttg ctgcgtcgta ggaattgtcc gacgccaccg acgcgcatac 7921 gtcgaccgcg atgatcgctg ctccctcgcg cgccatcttg accgcgtgcg cacgtccttg 7981 accgcgcgct gcgccggtga tgaaagcaac gcgaccttcg agcgaacgaa ctgaaccctg 8041 tgtcatgaga tagccctctc tgcagaattg aaactcgttc tagtagtaaa ccctgcaaag 8101 cgcccttcgc ggatggattc gagaacagtt ctcggggcaa ccgagtcgcc cacctgaatg 8161 tcggcctctt ccttcagcgt ttcgttcggc agacgaggcg agcagtcgat caccacttcg 8221 cactcgagct gtgaccgctc gccggtatgg acgtgttcga cgcagacggc ccctcgagcg 8281 atttcggtga cacgtgacgc gagcaccctg gtgatcccgg cctgctggat gcgcgtgttg 8341 gcgccgacca gatccccact cgcgccgagc ctcgaaccga cgatgctgtc gggagtcaca 8401 atcgagacgg agatgccatg ggccgcgagt gcttctgcga gcgccaccgc gatcggtcca 8461 ccgaggggat cgatcagcgc gaccggaccc ggccccacgg tgctcaactt ctcgaaggct 8521 tccgacgcat ccaaccaccg aaccgaatcg tcgaccggat acgtcggcgg cctcgcagca 8581 ctaccggtgg caacgacaac aaactcgccc cggctgcgtg cgtcacccag gtcttgggcg 8641 tcgagcgccg ccccggtccg catcgtcacg ccgctacgac ggcattcgtc ctccaaccac 8701 tgcgcgagga gtgcgaaccg gctacggccg tcacctcgcg caaaagttcg gatcatgccg 8761 ccgaactgcg ataaactctc gacgagtgtg accgagtgcc ccgccaccgc cgagatccgt 8821 gcggcttcca tcccagcggg gccacccccc acgaccagca cggctcccgg tgtcgaaatc 8881 tgctctcgct catcggggtc caccgtctcg aaacccgcgc tcggattccc gacgcacgtc 8941 acgacaggat tgcgaggatc acgcacgagg cacgtctgat tgcagagcac acacggccgg 9001 ggacgatcgc cgcgcgaggc tttcacaacc aggtccgggt ctgcgatctg ggcccgggtc 9061 atctcgacga actcacctac ttccagcgcc gcgtgagcgt cggcgatgtc cacgacgctc 9121 ccctgcacga caacaggaat cgacagagcg accttgatct cacggcacag atcgaggttg 9181 aagttcggcg ggcagtgaaa atcggggcgg taccggtcag acgaatagag tccgccacga 9241 acgacgacaa ggagatcgag gaacggagac acttcacgcg cgataccgat ggcaagatcg 9301 ggcgtcaggc ccgcccatgg agcttcctcg tcacaggaca accgcaggga gacaacgtga 9361 tccgacccga cttcctcgcg cactgccgag atgacctcgc gaacaagagc aacgcgatcg 9421 ctaccgtatc ggtcttgccg agtgttggtc agacccgaaa ggaattgtcg cagaatgctt 9481 cgaggaccgg catcgagttc cactccgtcc attcccgaag cgacggccag cgccgacgcc 9541 tcacggaagc cgactatcag ctggtcgatt tccggctgcc ccattgccat cggcaactca 9601 cgcgtcgccg ggtcggccac tcttgacggt ccccacagca ccgactggga gtatgcgctc 9661 gatccctgca tgccggtatg accgagcccg gccagaacca gcgttccgta cggagcacat 9721 gcagccgcga tcgactgcca ccccgggcca cattgcgacg ccaacggcgc ccgctcgtac 9781 ggccagtcgt tctccgatac cgaggccact tcggtgacga cgatcccggt tccgccctcg 9841 gcgcggcgct ggtagtacgc acggtgccga tcggaaagcg agcgtcccac agcaaggttg 9901 gtctcgtgcg gaccgaaaac aaccctggac cggacgaggc gtccggccag ggccgtgtgt 9961 tcgtcgagtg tcaactcaca gctctatcgg agccgtaatc tcagctggca cccaatgtgc 10021 gtgtgaaccc ttccggtatc accaggtccg ccgggctcag ttcggtgatg gacgagtggc 10081 cgaggcccat cagccccgag tcgatgccca tccgcatgag gtcgagcacg ttctcgacac 10141 cggcctggcc gttggccgag aggccccaca ggtaggcgcg cccgagcatg acggccttgg 10201 ctccgagtgc gagcgccttg acgacgtcgc cgccgcgtcg aatgccgccg tcgagaacaa 10261 cttccacctg atcgcccacg gcctcggcaa ttcccgggag cacacggatc ggcgccgggg 10321 tgccgtcgag gttgttacca ccgtggttgg agaccgagat tgcggagaca ccggcgtcga 10381 cagctcgctt ggcgtcgtcg atgcgcatga tgcccttgag catgaacggt ccaccccact 10441 gctcacgaag ccaggcgatg tcttcccacg tcggaagcgg agtctgcatc cactcgccgt 10501 atgcgccgaa gaacgtcggt gcaggctggc cgggagccgc gaggttgggg gtggtcaggt 10561 ccgggatctt gccggtcttg gcgaactcga gcaaccactt cgggcgcatg attccctcgg 10621 gtgcgaactg gaacatcgcc ttgaggtcca tcttttcggg gatggacggg ctgccccagt 10681 cgcgaccgta cgagaaggac cagtcggtgg tgatgatcag gcccttggca ccggcagcgc 10741 gagcgcgctc catacgctgc aggagcacgt cgcggctgcc gacccaatac atctggaaga 10801 agacctgcgg gttggccgcg gcaacttctt cgatggactt gctggcgaaa gaactcagtc 10861 cgatggctgt tccccgtgct gcagccgcac gggctaccgc tacttcaccg tcggggtgca 10921 ctgcctgcac accggtcggt gaaatcatga cgggcaacga aatgtcctgg cccataatgg 10981 tggtggacat ctcgcgcttg tccgacagcc cggcagcgtg gggagcgaaa cccaattcgc 11041 tgaatgccgc gacgttgtcg tcgaccgtca gccctttctc ggatcccgct accaacgccg 11101 cgtacacgga cttgggcaac ctcttcttcg cacggcgctg tgcttctgcc accgtctcga 11161 agaaagcatt cttagccatt gaactgacct ctctggggtg cagctttcgc cgcactgtcg 11221 gtatcgcgca gtagttactg cattcccgca agcgggtttt cgtcacaaat cttggcgggc 11281 ggacgcatca tcagtgtcag tggcaccgat gcgcgtgggg tcttacgttg accggaacgg 11341 gagtggtcga cactggactt cgggacctcg cccgccgacg ccagtgccat ttcaccgtta 11401 ccgatgacgc actcggggtc aggtccgtcc atcggcagtc cggtgaagaa cttggccgcc 11461 atgcagccgc cgcggcagga gtcgtagtgg ttgcacttgc tgcaggcgcc accggtttgg 11521 ggcgagcgca gctcctcgaa caggtccgag tgctgccaga cttcctggaa tccaccgtct 11581 ttcacgatgt ttccggcgag gaactgctcg tgaatcgcga aggggcatgc gtacacgtca 11641 ccgaccgggt cgatcaggca cacgacgcgg cccgcgccac agagattgag accgggcagt 11701 gcatcgccga aggcggagag gtggaagaac gagtcgcccg tcaggacgcc ttcgccattg 11761 gcgaccagcc agttgtagag ctcacgctgc tgctccggac gcgggtgcag gtcgtcccac 11821 acgtcggcgc cgcggcccga aggacgcagt cgagtgatgc gcagggtcgc gtcgtacttg 11881 tccgcgagag ccttgaactc gtcgagctga ctgacgttgt gccgcgtgac gaccaccgaa 11941 atcttggcgt ccttgaagcc ggcttccttc aggttcgcga gagcgcgctc ggccatcgca 12001 aacgaaccag gcccgcgcac agcgtcattg acctcagcgg tggcaccgtc gatggaaatc 12061 tgcacgtcga cgtagtcact ggctgcgagg cgcgccgcga ccttcttgtc gatcttcacg 12121 ccgttggtgg agaacttcac accaacctgg tggtcggtcg cgtagtcgac gagctcccag 12181 aagtcggagc gtaccgtcgg ctcgccgcca ccgatgttga cgtagaaaac ctgcatacgc 12241 tgcagctcgt cgatgatgga cttgcactgt tcggtgctca gctcgttggg gtcacgacga 12301 cccgacgacg aaaggcagtg cacgcaggac aggttgcagg cgtaggtgag ttcccacgtc 12361 aggcagatgg gggcgtcgag tccgagttcg aactgatcga cgaggcgacc caccttggcg 12421 ggcggggagg gctgttcaag aactgaggtc atggctgtgt cctcgcgaag aaatgaagcg 12481 ggcgaacgtg cggaagggct gttccgcacg tgggggcgag cgtctggctc agacaggcac 12541 gatcatgtgc gagtcggcga gagtgctcag cgcgcgggca tattggggag ccgcgtcatc 12601 ttcgatgcct tgcgcacgta gtgcggaccg gacatcagga tggtctggca acgccttgac 12661 caccgcaacg atggtcgtgt tcttcaggaa cgacagcttg cgtgttccga agtggtagag 12721 caacgccccg aaaggttcgg ggcgaagcgc tacctgcggg tggagtttcc accccgcatc 12781 gagatccaaa gcagacacgg tcagtacacg ccgcacatgc cgtcgatgga aacttcctcc 12841 acgagggact cctcgacgag gtcgttctcg agcgccttgt tgtcacgatc tgacatggat 12901 tcctcctgcg gtcgcactcc gtctggaagt ctgtagatca gacttccaac ctgggagttg 12961 gtggtcacta gtgggacttc actgtgactc ggttcaccac tgtaatggca tcgagtgcag 13021 aatgacacac atggagcgaa aaaagtatgc gtagccgcaa gaaaccgtcc gctcggatag 13081 gtcgtcgccc ctccacaacg aaggagggaa tcagcacgat cgggatcgaa ctgttctcga 13141 cagtcggctt caacgaggtg agcgtcgacc agatagccga cgccgccggc atcgcgcgtc 13201 ggaccttctt tcgctacttc ccgtccaaga acgccgtccc ctggggagac ttcgacggac 13261 atctcgacga gatgcgcgag cacctggatt cactgcccgc tgatattccg ttggtcgacg 13321 cactcgaatc cgcattgttg acgttcaaca ccttcccacc ggaagagact gcggtacatc 13381 gcaaacggat ggaattgatc ctcgaagttc cgacgcttca ggcatactct gtggtgatgt 13441 acgaaggttg gcggcaggtg gtcgccgact acgtcgcacg ccgaatgaat ctcgagccga 13501 ccgatcacgg cccacgcacg gtcggctatc ttctgctcgg ggtcgcgatg tcggcgtacg 13561 agcagtggct caccgacgat tcactcgagc taccggaact cttgaaaaca ggtacgcagt 13621 ccctgcggac cggtatcagc acctgagaaa gcatcaccac ctgagaaagg cgatcatgtc 13681 caccatcacg aacgaacccg tcctggattc cctggcgccg tcgacgccgg gcttgtggtc 13741 gttcgggcat gtcaccgtcg aacgcgtcga gtccggtccg atctcgctcg atcgccgagg 13801 cggtcgcctc cacgtcaggc actccctcac tcccgccgat ctgagtgagc gactcgtcgc 13861 cggagtcacc tcgtcgatcc aggacgcgga gttcggtcag gacgaattcg agttgacgat 13921 ggtcggcctg gttcgctcca gcgtggacgg cgctctcgaa tcctggacca cctactaccg 13981 gaactccctt gccgaactac tggacggcac tgcagatttc gcgccgattc acgacaaagc 14041 cgaagagctg gtccgcggtt ccgtgctcga tctgggttcc tgcttcggtt tcctgcccct 14101 gcggctcgcg cgcgccggaa tggacgtgac cgccacggac atcctgcccg gaacgatgat 14161 cctgctcgac gcggttgccc ccgaactcgg catcacgatc gacacggtga tctgcgacgc 14221 gggtgaagta ccggcaccgg acaacagcgc cgacaccgtc accgcgattc atctcctcga 14281 acacgtcgac gacgcagtcg gcgaggccgt gatcgcgcaa gcactccgga tcgcgcgtca 14341 acgggtcgtc attgccgtcc ccttcgaaga cgaagccacc gcgtgccacg gccacatccg 14401 cactttcgat ctcgagtcac tgcgtgcagt cggccggcag agccgtgtgc cgttcgaggt 14461 attcgagcat cacggcggct ggttggtgct ggacgcatcg gccgcctgaa aagaactagc 14521 cttctttcag tgtatgggcg acaagcgcat tcgcgtgccc atgacccata ccgtgctccg 14581 acttcaacca gttgaccaat tccatgtgct tcgtgaggtc tgagcttctg atcagctctt 14641 gccattcacc gatcggccga ccgtacttct tctcgatcga cggaaaatac gacgccgggc 14701 ctttcacggg ttcagtcatg accggaatct acctccaccg cccgacaata ttcgcgcacc 14761 ggcggcgccc gcatgtccgc acctgcggaa tcgctgggta gacacgcgac catcgcatta 14821 tggtcgaaga acacccaccc gagagcacag gagttcagcg atgcgactgt ccacacgaaa 14881 tcaactcacc ggcaccatca ccgaggtcaa cctcggcacc gtcatggcga cggtcaaggt 14941 gaagttggac ggcgggaatc agattgtgac ctcgtcgatc acccgcgatg ccgcactgga 15001 tctcgatctt gaaccaggcc aagccgcaac ggtattcgtg aagtccaccg aggtgacgat 15061 cggcgtcgag taactggcga gcgatgtgat cagggccggg tgagatgcgt ggtcatcgtg 15121 tagtccttgc tcggccccgt cacggtccag acgacactcc aggtcgatcg atctccggca 15181 gtgacgtcga ttcgcccgcg gtaggtatcc gcaccgcaca ggtgctcgac ttcctccccc 15241 ggcgcccagt cgtggaactc tctgccgtct tcgaatgtca cggcccactc gaggccgcgc 15301 tcggcaacga acagagttcg gaacacgggt aattccaggt cgccgtcacg cagcgtgccg 15361 ctctcgtacc agcgcatccg accgtcaccg gcgtcggaga tagtggtctc gccgaataca 15421 tgcttggtgg aaccggaaag gcggtcgacg atgtcccgct cgaagtgcca ctttcctacc 15481 agcgccctcg gctcgatttt cgcgtcgtcg accatgtggc gattctagtg accctgcatt 15541 gcccgacgac accggagacc gactcgaaca cactgcgaga gtatgttccg agtacacccg 15601 gaacacgcag gagattttca caaccatgaa cgattcggcc gcccttcaca aacgcgcagt 15661 gttcgccgag gatctcgaga tcggccaagt gatcccattg ggttcgtatt cggtgaccga 15721 agaggaaatc gtcgagttcg caactcagtg ggatccccag tggtttcacg tcgacaagga 15781 agccgccgag tccggcgtct tcggcgggtt gatcgccagc gggctgcaca ccctgtcgat 15841 ctgccagaag ttggtggtcg ccagtctcta cgaccagtgg aacgtgatcg ccgggaaaac 15901 catgcgcgac gtgagcttcc tccgtccgct tcgccccggt gacaccctga gcggaaaggt 15961 caccgtcgac aacgtcgtgt tcgacaatcg cgcgcgagga ttggtgacca tcacagcgaa 16021 attggtcgat gcacacggca atcgcgttct cgacgtggtc actgacgcct acctgcactg 16081 ccgctcgact cgcggacagt gaattatcgg taaatttcgc accacactgt ccgaattgcc 16141 gtactttcag tgacgttgcg acgcaacaat acccatccct gcaatcagga gttcctcaca 16201 catgagcagc ctcggcgaga tcaatcctct cggcattctt cagatcctca tctacatgct 16261 cttcggtgga tcgtccagca ctcccgaata gaccctagct tgccatagcc atagtcagca 16321 tctagggtgc tgactatggc aattactagt caggtatcag tggcggccgc gccgtcgacc 16381 gacgcagcgc gtcatcacac cgaattcgac tccagttggc ggagtttcga cggcatccag 16441 ggcggactcg caattgcacg gatgctgcga gtggcggccg acgcgtccgg agccgaaccg 16501 ctggcagtca cggcgcacct ggaccgcccc gttcctcctg ggccggccga gttgactgtc 16561 ggcgagatgc ggggcgggcg gaccgtcagt tgccacgtca cgctgaccgg ttcgggatcc 16621 gctctcgttc ggttggcccg cgacatagga gctacaacaa tgtccgcccc tgtcgccgaa 16681 ccggctccgg ccgacccgtc aaccctgccg ccgctggaga tcccggtgga ttttgtgccg 16741 ttcgcccagc acctcgacat ccggccgatc aattccgcga ggccgttcgc cggtggcgac 16801 tcccctgaat tcgacgtctg gatccgcctg ctcacagacc tcgatttcac ggcccaagag 16861 cgtgccgcgg tccttctcga cgcgcttccg cccggactgt tcgcgacact gacgatgccc 16921 gtggccatac cgaccatcga gttcaccgcg catttcacac cggagcacct cacggcagat 16981 ccccgaacgc tgtcaccgtg gtatcgccta cgccacggga ctgcctgggc gacggacact 17041 ctctgtgttg acgaatccga actattcgac tcgaccggac gacttgctgc tcgggctcgc 17101 cagcttcgtc gaattctgag ttgagtttca gctctcctac acctggaggt cgcgagcacg 17161 gaacgcaaca agcgatgctg tcgaaagcac cactgccaca acgatcattg ccagcagtgg 17221 cagaactgtc accggctcgg caggggcacc cggcacgtgg gtgaagggcg acagatccag 17281 cactacctgc ggaagcttca gcaacgtgcc gagttgcccg agcacgatgc atgccgccaa 17341 gattccccag gagagcgccg acgcgtacgt cggaaacagt ccgaaagcca acgacaccaa 17401 ccccacgact acgagcaccg caggcagata ggccaacgcg gcgccagtca tgcgaagtgt 17461 gttgccgccg atgtcgtccg tgacgagccc gaaggcaaaa ccggtgaagg cacccatcaa 17521 gacgtgcgcg aggatcacgc cgcccacaac cacacccagg tgggaaagca tccacctcca 17581 ccgcgacaaa gacgttgcca gcaacggttc gagccgtccc gatgtttcct cgccccgcat 17641 ccggagaaga ctctggaccg aatacgccgc gatgactatc gacgtgagcc ccagagcagt 17701 ggccaagtag gaatccacca gatccgatcc gccgccgcca agtttctcga acatctccgc 17761 ggtctgctgc gacgatccca ccatcgcgtc gatctcgttg cccacagcgc cgtacgcgaa 17821 cccgagtatg acaacggcaa agatccacca gaacatcgtc attcgctgca tgcgccacgc 17881 caggccccag gccgacggta gccacgatgc cgcgactggc gggccgggtc ggggctgaac 17941 gagtcccgct ccctgatcgc gatggccgat gagaacaaaa gccaccacca cgtgcacagc 18001 cgcaaatccg agcagcaaca acaggatcca ccaggcgtcg tcgtcgaagg gtcggatctg 18061 ttgtgcccac ccgatcggcg agagccacga caaccagcca ctgaccacgc gagttccgtc 18121 gtcgacgacc gtactgttca cgtcgccgat cgcccgcagc atgaaggcaa cgccgatcgc 18181 ggcacccgcc aggccgttgg ccgtacgagc accgtcggtg atctgcgccg tcacggctgc 18241 cactccggca aacgcgattc cggtcccacc tatcgaggca cccaacgcga acgaaccgac 18301 gacaggcaaa ccgacggcga tcgatcccag tgcaattccc agtgccagaa cgacgttcgc 18361 accgaccgtt acgatcagag cggaggtcag gagcgctcgt ctacctacca ccgctgcacc 18421 gatcatctcg gctcggccgg tctcctcgtt ctgtcgcgta tggcgaacca ccgcgagggt 18481 gctcatcaat gcggccacca acgcgaacac gagcaccgac tggctggcga ctatcgcgcc 18541 ctgcgaatca ccggacacga tcccgttggt cgccagcgcg accggcgata ccgcggaact 18601 gactgcgatg tttcgtaatt cctcgggtgt cgagtacagc ccggtcacac tggcgacaac 18661 cagcagcatg aacaagacga tggagataac ccacacggag agctgaattc ggtcccgccg 18721 caaagccagc cgaaccagtc ggcgtgtgcc ggtaaattga cttgccgagg ccggtaccgg 18781 tggagcggcc tcgacaacaa cggtcacggc cgtactccgg tctcggctcg caggtgtacc 18841 gaagtctccc tggcccgttc gtcaccgtaa tgccgcaaga aaagttcttc gagagtcggc 18901 ggagtggaag tgagcgccac gattccgaat ccgaccaagt acgtcatgag tggtccgagt 18961 tgcgcgctgt cgacttcgca ggtgacgtgg ttaccgtcga tcaccgcgtc gtggacaccg 19021 ccgagtccgt cgagtccacc gatcggaagc tgcgtctccg ccgcgacgga cgtgcgcgtg 19081 agatgcctga gttcggacag tgttccctct tccaccgtcc gcccgtcgcg gatgatgctc 19141 acacgatcgc acagcgcttc gacctcggcg aggatatggc tcgacagcag taccgtcttt 19201 ccggcgtgag ccacctcctc gacgcagtcc tggaatacgg cctccatcaa cgggtcgagc 19261 cccgatgtgg gttcgtcgag gacgtaaagg tcgacgtcgg acgagaacgc ggccacgagt 19321 gcaaccttct gccggttacc tttcgagtac gtgcgcgcct tcttggtcgg gtccaattcg 19381 aagcgatcga gcaactcgct gcggcgtttt cggtccaggc caccgcgcag gcccgccaac 19441 agatcgatag cctcaccgcc ggtgaggttg ggccacagat tcacgtcgcc aggcacatag 19501 gcgagacgcc gatgcaaagt aacagcgtct cgccacggat cgccgccgag aacggtcgcg 19561 tctccggagt ccgagcggag caggccgagg agcacccgaa tagtggtgga cttgccggaa 19621 ccgttcggcc ccaggaatcc gtgcacttca cccgtcgaga cgtcgaggtc gaggccgtcc 19681 agcgcgcggg tggtaccgaa ggtcttgacg aggcttttca ctgaaattgc cgcacccatg 19741 aattttaccg tacgccgatt tcacaaatgt gtgaagatag aaaagacgga aagatcgaag 19801 ggttccgatg accgacacca gccacaccgt tgcaccggac gagagccgac actatgtcga 19861 gcgactctca ctcgtactga gcgacatggg ttttccgccg atgcccgcac gcgtctgggc 19921 cgcgttgatg accagcgaga caccctcgtc cacgccagga gatctcggat ctcggttggg 19981 agtgagcccc gccgccattt ccggagccgt cagatacctc cagcaagtcg gattgatcga 20041 gcgtgtggcc gtccccggat cgcggcgtca gcactactgc gtcagcgtcg acatgtgggc 20101 cgacgcgttt gtcacgaaac agtcctcgct ctaccaggtc tcggcagttg cggacgaggg 20161 catacgagtc ctcggtgaag gaaccacagc cggcgatcga atggcagatc tgcgcgactt 20221 cttcgacttc atggccgcgg aaatgccgcg aatgctcgcg gactggcgct cccggcgaaa 20281 agaccggaca cctggcaggt aacttcaggt ttacccgcaa tttcggctgg gtacctcaat 20341 tctcgttcca gcatggtcag gtgcggtgta atcgaggagt tgctgatgaa gctgtcgatc 20401 aggggcgcga acacttcacc gactccaccc ttcgcgcgta ccgcttcacc cgaaacctgt 20461 gaacgatcga tttccctgaa tcgcaccgcc gccgaggcat tcgccggtga tcaccatgca 20521 ttgtcgaaac tactggcccg gttgcaccct cgcgtcgttc tctactatcg cgccagactc 20581 ctcgggacgc ccgtcgcgac gttcgccgac gatctcgccc accaatgcac cctggacgtc 20641 gtcacacgaa tgtccgacac gatctcggcc gacacacgca tcgagacctt cgcgtacgag 20701 ttggcctctc gacgaattgc gtcaattccc cagtcacgca acatcgacgg acaattcaaa 20761 cgcccgcgcc gcctgaccga cgaccaacgt gaggttctgg tactacgaat cgcagtgggt 20821 ctgactgccg agatgaccgc atcgactctg cgcacaactc gttcgagcgt gctcctccta 20881 cagcatcaag cgctgcgcgc ctggacggtc tccgagcaga gctagatcag accttgctta 20941 ctcgcggcgt agacggcctc tgctcgacgg ctcacctcga gtttgcgcat gatgttgctg 21001 acgtgaaact tgacggtggt cggtgagatg aacaatctct caccgatttt gatgttggac 21061 aagccgtttg cgaggagtct gagcacttcg agttcgcgat cggtcaatcg ttctttcgac 21121 gacgactgcc cgttgagcga tcgcacgaca gctgacgcgc tgcgcgaatc gaacgcactc 21181 tcaccccgcg agaccgccct gatcgcacgc accaattcgg tggtgtcgac gtctttgacg 21241 acatagccgc gagcacccgc gtgcaccgct cgcaccacca gttgatcgtc gagaaacgtg 21301 gtgagaacca acagtccgag gccgggatgc gccgaggaca gctggccaca cagggtcagt 21361 ccctcgtagt cggaactggc ggagagtttg agatccatca ggacgatgtc cgggccgacc 21421 ttgtcgacca cggcgagtgc ctcggcgagg gacgaagcct cccccacgat ctcgagatcg 21481 ctttcgcgct cgagaaccga gcggagccct tgccgcagaa ttgcatggtc gtcgaccagg 21541 acgattcgaa ccaatccctg aaccggcgat gctccgcggg tgatgacggg acgcgcagtg 21601 gtcatgattc ctcgctctcg atttcagttg tggagcaaag gttttccatg cccgagtcga 21661 acggcagtgg tagtcgcacg agaatctgaa tcccaccgat ccgcgcgcgc cgtacttcga 21721 gcgcaccacc caactcgagt gttcgcgcct gcatgttcgc gagaccgcga tggcggccgt 21781 cgacgtcgtt ggtggctgcg agtttgagga tcagacgcat tcgcgcaggg tcgccgtcac 21841 cgtcgtcgga gaccgacagc aacacggagt ccgcccgata ggtcagacgt agcaccgccc 21901 gattcgcgtg agcatggacc gctgcattga agagagcttc ccccgcgatc cggagaagag 21961 cgtgttcggc atcgcccgga agttcggtcg gggtaccgcc gacgcgaacg ctcaccgcca 22021 actcgtccgg catgtgaacc acgccgagtt gctcgagcat ctcgggcaac gacgtccggt 22081 ccgcgtccga cgaatgattg agcgcgtaga tcgccgaacg aagctgctcg accgccctcc 22141 tggtcaaatc ctttgccaag tccagtcttt cactgcgttc agcctccggg atgtcactcc 22201 ggcagacctc gatctgcatg ccggccgaca acaccgcctg tgtcacactg tcgtgcagtt 22261 cgcgggcgat ccggtgacgt tcgtcgtcga ccacttggtg ccggtgcgcc actccgagtt 22321 gacgctgagt ggactccaat tcgctgttgc gcaccgcaag gtcggctgca gtacgattgg 22381 cctccgcata cgcatcttcg gcacgctcga gcagtagttg tccgcgctga tagagcgccg 22441 aattctgcag agccacagca gtctggctgg ccagaatgct gagcacggct ccgtccgtcg 22501 tgtcgagggt tcggttggcc ggcgtccagg ctgcaaaggc acctaccacg ccgccaccga 22561 gcacgatcgg cacaaacgcg tagtgcgcgt cgatcaccga gttggattcg attcttccgt 22621 accggatgtc gtcgaggcat tcgacgacat cctccggaag tggcggtccc tccacattgt 22681 cgaccagaaa aggggtggcg tccgggccga gaaccagtcg ccgtggtccg gcgtcgggca 22741 aagttccgtc agccaaggcg aagacaatcc agttggcact cagatgcgca cgggcagctt 22801 ccgcgaccgc gcagac //