LOCUS       NZ_JARBUT010000026     19796 bp    DNA     linear   CON 29-APR-2024
DEFINITION  Nocardiopsis sp. N85 Scaffold26, whole genome shotgun sequence.
ACCESSION   NZ_JARBUT010000026
VERSION     NZ_JARBUT010000026.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nocardiopsis sp. N85
  ORGANISM  Nocardiopsis sp. N85
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsaceae; Nocardiopsis.
REFERENCE   1  (bases 1 to 84100)
  AUTHORS   Liu,S.
  TITLE     Nocardiopsis sp. N85
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 84100)
  AUTHORS   Liu,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Microbial Chemistry, Institute of Medicinal
            Biotechnology, Chinese Academy of Medical Sciences, Tiantanxili No.
            1, Beijing 100050, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 1.05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 182.0x
            Sequencing Technology :: combinatorial probe-anchor synthesis
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028882275.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 06:01:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,663
            Genes (coding)                    :: 5,611
            CDSs (with protein)               :: 5,611
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 46 of 53
            Pseudo Genes (internal stop)      :: 0 of 53
            Pseudo Genes (multiple problems)  :: 3 of 53
            CRISPR Arrays                     :: 13
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:47:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 19796
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JARBUT010000026.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..19796
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lassopeptide"
                     /region_number="1"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..19796
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..19796
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[7487:9796](+)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      7488..9796
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     gene            complement(649..1125)
                     /locus_tag="PWG71_RS25920"
                     /old_locus_tag="PWG71_25920"
     CDS             complement(649..1125)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017566911.1"
                     /locus_tag="PWG71_RS25920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_25920"
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="WP_274812639.1"
                     /transl_table=11
                     /translation="MSTPDMTGSNDSARDSSLSHLPNHIRQAIEITPAAGTRERTIDIT
                     TLGRRTSRPRRIEIFFYRAAGTTYLCSGAGGAATDWHANLLANPDFTFHLKNGIRADLP
                     ARATPVTDPAERQAVLAEIVADLNQPHDPGTIRPTRLEDWADSRLMRISFRHQP"
     gene            complement(1122..1901)
                     /locus_tag="PWG71_RS25925"
                     /old_locus_tag="PWG71_25925"
     CDS             complement(1122..1901)
                     /EC_number="1.1.1.47"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (7-167). E-value: 6.8e-10. Score:
                     31.1. Matches aSDomain:
                     nrpspksdomains_PWG71_RS25925_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017566912.1"
                     /locus_tag="PWG71_RS25925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_25925"
                     /product="glucose 1-dehydrogenase"
                     /protein_id="WP_274812640.1"
                     /sec_met_domain="adh_short (E-value: 3.3e-32, bitscore:
                     104.1, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPRFDNQTVLVTGGTGGQGASHVRAFHAEGANVVIGDIDAERGAA
                     LADELGTRARFVRLDVTDQSSWAAAVLAAESAFGALNVLVNNAGVQNPPALIEHTDQAT
                     WSRILDINLTGIFLGIKVAVPALRRAAGGAIVNIASAMGLGGTAHYAPYVASKWAVRGL
                     TQTAALELGRDHIRVNTIHPGVISTPFIHKPAVGATAAITDFYSPEPFAIPRLGEPTDV
                     SRLLLFLTSSEASFITGSEYVIDGGLLLGPALQSETV"
     aSDomain        complement(1401..1880)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PWG71_RS25925_PKS_KR.1"
                     /evalue="6.80E-10"
                     /label="PWG71_RS25925_PKS_KR.1"
                     /locus_tag="PWG71_RS25925"
                     /protein_end="167"
                     /protein_start="7"
                     /score="31.1"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TVLVTGGTGGQGASHVRAFHAEGANVVIGDIDAERGAALADELGT
                     RARFVRLDVTDQSSWAAAVLAAESAFGALNVLVNNAGVQNPPALIEHTDQATWSRILDI
                     NLTGIFLGIKVAVPALRRAAGGAIVNIASAMGLGGTAHYAPYVASKWAVRGLTQTA"
     CDS_motif       complement(1767..1880)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PWG71_RS25925_0001"
                     /evalue="2.00E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="PWG71_RS25925"
                     /protein_end="45"
                     /protein_start="7"
                     /score="10.9"
                     /tool="antismash"
                     /translation="TVLVTGGTGGQGASHVRAFHAEGANVVIGDIDAERGAA"
     gene            2043..2687
                     /locus_tag="PWG71_RS25930"
                     /old_locus_tag="PWG71_25930"
     CDS             2043..2687
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017566913.1"
                     /locus_tag="PWG71_RS25930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_25930"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_274812641.1"
                     /transl_table=11
                     /translation="MSRAVTTYHQRVAQEKRALIVTAATALFLELGYDRTSLARIAESS
                     GVSRATLFKQFPSKAALFDAIVTESWSTADEEDPPPAGNVVDGLSIIGRRYAELLGRAQ
                     MTDLFRIVIAELPRFPELAHAQFSHGKLPYFESLRSYLQAEHEAGTVRIEDVDLAATQF
                     LGMISNYVFWPTLLVPDWEVSAERTAQVVDEAVRTIAARYAANGPGPSTNS"
     misc_feature    2340..2342
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            3123..3590
                     /locus_tag="PWG71_RS25935"
                     /old_locus_tag="PWG71_25935"
     CDS             3123..3590
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PWG71_RS25935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="PWG71_25935"
                     /product="hypothetical protein"
                     /protein_id="WP_274812642.1"
                     /transl_table=11
                     /translation="MKRRTLIGVIGVGLAVLPVGWALRQAVNGTKEDWRTDTAPLERAF
                     PGLGSLTDAKWVSTRDNDRLLQTPDLVFAGFARLSPGKLDELTTSHAFVSEEPTFDFSS
                     GFTKPLADEGPDDPQWIRSPELDRSSDNHSTSLWFDRRSDTVRFWASNPYG"
     gene            3753..4889
                     /locus_tag="PWG71_RS25940"
                     /old_locus_tag="PWG71_25940"
     CDS             3753..4889
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540506.1"
                     /locus_tag="PWG71_RS25940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_25940"
                     /product="IS701 family transposase"
                     /protein_id="WP_274812707.1"
                     /transl_table=11
                     /translation="MFSQVVAPFFGRREPRLRARSYLLGLLSSLERKNGWSLAEYASDA
                     TPDGMQRLLNHARWDAEQVRDALRAQVGERLGHLDGVLVVDDTGFEKKGRRSAGVQRQY
                     TGTAGKITNCQIGVFCSYVDPDRQRVLIDRELYLPRSWFTDPDRLTDAGVPERAHFATK
                     PELAWAMIERAADDPLLSFAWITADEAYGDNTRLRERCRDRGLSHVMAVSRDHRLVLGG
                     VRTRADTVVSLLAPDAWQRYACGDGAKGRRFYDWAWIGLGGHAWVLARRSISDPTDLAF
                     YRCWSVRPVALSELVRVAGARWSVEEVFQTTKNEVGLDHYQVRTHTAWYRHITLAMVAH
                     AHLAFLAADPPPGREGPVGEETDGLATQGARPVGTIAA"
     gene            5208..5738
                     /locus_tag="PWG71_RS25945"
                     /old_locus_tag="PWG71_25945"
     CDS             5208..5738
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017571166.1"
                     /locus_tag="PWG71_RS25945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_25945"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_274812643.1"
                     /transl_table=11
                     /translation="MSTQWTIHGEKLVDQNRHIRLSTVDITLPDGVSFTQYVVAMPPAA
                     MTLVVNEQREVLLMRRHRFIIDRWVWELPGGYVDGVENIEAAAAREVEEETGWRPRSIE
                     HLVTFQPAIGSVDQPQIIFLARGADLTDTAPDINEAQEVRWWPLEEAVEMIARGEIIGA
                     STVVALYRALTLV"
     gene            complement(5725..6519)
                     /locus_tag="PWG71_RS25950"
                     /old_locus_tag="PWG71_25950"
     CDS             complement(5725..6519)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PWG71_RS25950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="PWG71_25950"
                     /product="hypothetical protein"
                     /protein_id="WP_274812644.1"
                     /transl_table=11
                     /translation="MQYLDANVAPLLRGTYKEEVGRDLFSAAAELTKTVGWMAYDCEEH
                     GLAQRYLIQALRMAQTAGDDGLCAEILAAMGHQATYIGRSAEAVDLARAAQSAAHRSGH
                     PALAAECHLIEAHGHAGLSDPRATSRSLRAGVKAFEADAPDPPEWLAYFDHAYLAAKVA
                     HCFLALGNDSQTAAYAAQSLKMNTDYVRGKTFNLLMLATARATDEPEEAVRVGGMALDL
                     VEGLQSQRALSYLRRLRHRLRPHEGLPTVEEFTTRAEELTPR"
     misc_feature    complement(5842..5844)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(6319..6321)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    7255..7269
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.83"
                     /tool="antismash"
     gene            7286..7414
                     /locus_tag="PWG71_RS25955"
                     /old_locus_tag="PWG71_25955"
     CDS             7286..7414
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017573784.1"
                     /locus_tag="PWG71_RS25955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_25955"
                     /product="albusnodin family lasso peptide"
                     /protein_id="WP_274812645.1"
                     /transl_table=11
                     /translation="MDTPEAEARELSEEVLVELGDAADLTLGQGRAQNENKRNPYN"
     gene            7488..9179
                     /locus_tag="PWG71_RS25960"
                     /old_locus_tag="PWG71_25960"
     CDS             7488..9179
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013155481.1"
                     /locus_tag="PWG71_RS25960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_25960"
                     /product="albusnodin/ikarugamycin family macrolactam
                     cyclase"
                     /protein_id="WP_274812646.1"
                     /sec_met_domain="Asn_synthase (E-value: 7.4e-27, bitscore:
                     87.1, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWFGGSTGPIPLRPANAVPVSPNVPGVWMSTEWPERELVTAAHGE
                     RHVLVFGTCAVESAELHRATAPAVPGDVAWRWPGSYVVVEVTPGSVTLWTDLTSALPLY
                     IRRTEDRVLWASSSRLLAGLYGSVEVDLAKVVERLIGPDTAEHGTGSYFTGAERVPAGH
                     RLVLSKGTEPVAGRVWEPKDVGSGHGERLRSALEGAVAVRVDRAARPSTDFSGGFDSTA
                     LGLLAAERLAPRGREIVGVTVHPDGVLYGGDLDYAREAAQHPAVLHRLLPLGREHSPYG
                     GLDCVPPTDEPAPSTVSYAYFSAQLRWLSQEVDSDMHMTGDGGDALLLTPPYQLVELLH
                     RGRVRHAFQEAVRWAHVRRMSVLNALRAARSRADPDPVPTWVRITSPVPQGVEDASPRG
                     RTRRMLERTMIHAGRTARADVQLAQAAGVPLHNPYFDSQVVNAYLSVPMEDLPGPARYK
                     PVMADAMRDLFPAKLLARTTKGDASSDHHEGLRRSLPFLMEFVDGHLAQEGLINVSALR
                     NSLSRAALGIGEELAQIESTVATEAWLRTAHRAPAPEWFPDKAVLQ"
     gene            9362..9796
                     /locus_tag="PWG71_RS25965"
                     /old_locus_tag="PWG71_25965"
     CDS             9362..9796
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /locus_tag="PWG71_RS25965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_25965"
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_274812647.1"
                     /sec_met_domain="PF13471 (E-value: 5.3e-22, bitscore: 70.3,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGFSEFPSVPARYRIAATVALPVSLAVMRAGPRSARMGRMVRLVR
                     AWRGGGRMATLPEAENAVHAVRRLGLFSPVRVACLEESVAVVVALALLGKRVRWHHGVI
                     TDPIRLHAWVEAEGLPVAEPESTRRCTALLTITPTSEENT"
     gene            9793..10368
                     /locus_tag="PWG71_RS25970"
                     /old_locus_tag="PWG71_25970"
     CDS             9793..10368
                     /EC_number="2.-.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1111: GCN5-related N-acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013155483.1"
                     /locus_tag="PWG71_RS25970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_25970"
                     /product="GNAT family protein"
                     /protein_id="WP_274812648.1"
                     /transl_table=11
                     /translation="MSEPLIWLRGDRAGIGPLRADLVEEYWRWEQSIPTIVGYNRQTPQ
                     PVESTRDILLEGYARASDRELRFTVYGLTGQEPRPVGLAQVYIDQMRRNGEYVVALGES
                     RGKGVGTEATRLVLDYAFHVTNLRCVYLTVLAPNTGAIRAYEKAGFKRQGVRRNSNQWL
                     GETVDEVLMDAIPEEFEGSSLVKAQFQR"
     misc_feature    9844..9846
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(10406..10585)
                     /locus_tag="PWG71_RS25975"
                     /old_locus_tag="PWG71_25975"
     CDS             complement(10406..10585)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013155484.1"
                     /locus_tag="PWG71_RS25975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_25975"
                     /product="DUF397 domain-containing protein"
                     /protein_id="WP_274812649.1"
                     /transl_table=11
                     /translation="MSTQSTPWHKSSYSNEAGTCVEVSEGPVTRVRDNQNLHVQPLDFT
                     PGSWAHFLGGLNTD"
     gene            complement(10600..11454)
                     /locus_tag="PWG71_RS25980"
                     /old_locus_tag="PWG71_25980"
     CDS             complement(10600..11454)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013155485.1"
                     /locus_tag="PWG71_RS25980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_25980"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_274812650.1"
                     /transl_table=11
                     /translation="MPEGTPAHGSPARRHYLVSQLKRLRVQTGLSQEEAAEEMGWHKSK
                     VSRMENGKWKRLSSPEIDALCRLYKAADDLREELAHIAKAASRNRTSAWWNAYEDVPGG
                     AYYPLEYEAGQIFEYTIGLFGGLAQHPDYIRALMGRGAVPDEAERERRTQARLERAHSI
                     LHRDEPPQMWWVIDEPVLTCQVGGPGTMRAQLTHLLELSDHPRIDLQILPQAEGMSLNY
                     GFSLLWLGDDQVGHVDVPPQGLLFDAPEDVQHHELRSHHLRAAALSPERSRDLIRSRIA
                     ELD"
     gene            11654..12136
                     /locus_tag="PWG71_RS25985"
                     /old_locus_tag="PWG71_25985"
     CDS             11654..12136
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013155486.1"
                     /locus_tag="PWG71_RS25985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_25985"
                     /product="ATP-binding protein"
                     /protein_id="WP_274812651.1"
                     /transl_table=11
                     /translation="MHEVMCVWRRPGPEDVGLARCLTDGALAAAGVDEAGLGVARLAVS
                     EAFTNALLHGRPEVGVEVWARPGRCGVVEVYDTGPTVPVFPQPEELAWVTGDHGRGLGL
                     IRAFTREACGAYACGVGKRVWFAVAMDGRALDVHRTRALVEGRARLRAPRSTALLA"
     gene            12229..12750
                     /locus_tag="PWG71_RS25990"
                     /old_locus_tag="PWG71_25990"
     CDS             12229..12750
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013155243.1"
                     /locus_tag="PWG71_RS25990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_25990"
                     /product="hypothetical protein"
                     /protein_id="WP_274812652.1"
                     /transl_table=11
                     /translation="MRHPTTGRHRRPCDSMAGRVWALTAALLATALATIFSPRLDRPTQ
                     VPRALPAAPTPGGNPGHVKQGRDLLSDPPGFPEQSVWAAGASASPTRWEQAYRDRSTPF
                     AGEDVVDADPDQIAGALVRPYLDLAAVPRPRSAEPSDYPARSSAVESEPVEDEFAELAD
                     RIRTYLAIRN"
     tRNA            complement(13305..13377)
                     /anticodon=(pos:complement(13342..13344),aa:Lys,seq:ttt)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="PWG71_RS25995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="PWG71_25995"
                     /product="tRNA-Lys"
     gene            complement(13305..13377)
                     /locus_tag="PWG71_RS25995"
                     /old_locus_tag="PWG71_25995"
     gene            complement(13567..14088)
                     /locus_tag="PWG71_RS26000"
                     /old_locus_tag="PWG71_26000"
     CDS             complement(13567..14088)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PWG71_RS26000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="PWG71_26000"
                     /product="hypothetical protein"
                     /protein_id="WP_274812653.1"
                     /transl_table=11
                     /translation="MSHDPPPSHGAPSPGKAGSTGTQVALGLGIGCLGLVVAGSVLTIG
                     GVLLARELGQDLAPSPGVSSEEVPAEPTDGYTAQTLVAELDTLYPLPDARDESSSCASE
                     DDGCLALVTTDLVSVYEMPDADSAARRAAGTETGDARQAGRFVLFWPEDSAQSLLERDK
                     LHTKAIVLVG"
     gene            14352..15188
                     /locus_tag="PWG71_RS26005"
                     /old_locus_tag="PWG71_26005"
     CDS             14352..15188
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1171:
                     transcriptional regulator, MerR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /locus_tag="PWG71_RS26005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_26005"
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_274812655.1"
                     /transl_table=11
                     /translation="MFTIGEFASIGRVSVRMLRHYDRIGLLTPARVDPVTGYRSYEVGQ
                     LTRLSRIVQLRGFGLGLEDIARIVCDDPDPGREREILRAASAELRRDIVERRARLDRID
                     AYLRRREGLPAMSTSTTITAEVRWVGPQRIATLTREIPGPGPENVGPVLGPLYPEVAGV
                     LAAAGIDVGPGIAFYRYDESEEATGMTVTAGFVVPDGVDAAPGLEVSTLPGCEAAVTTH
                     RGEVAGIGETWAALVDWTREHGLGLAGVCREVYWTPGDRPQEEWVTDLVQPVRPAG"
     gene            15233..15751
                     /locus_tag="PWG71_RS26010"
                     /old_locus_tag="PWG71_26010"
     CDS             15233..15751
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013152617.1"
                     /locus_tag="PWG71_RS26010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_26010"
                     /product="DinB family protein"
                     /protein_id="WP_274812656.1"
                     /transl_table=11
                     /translation="MSDVRTDLLRRQFDLTWGLLEYHLDRLVPEDVLWEPAGLCWTMRP
                     EGDGWSPDWADTEPDPVPVPTIGWLSWHIGWWWEVTLDHLAGRVPRDREAVDWPGPEGV
                     VARLVGLREEWTRVLPTVDLDAPAPFPWPEGSGMTVADTAAWVNAELMKNAAEMGQLRM
                     LRAVSSPGS"
     misc_feature    15752..15771
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 22.98"
                     /tool="antismash"
     gene            15972..16304
                     /locus_tag="PWG71_RS26015"
                     /old_locus_tag="PWG71_26015"
     CDS             15972..16304
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543437.1"
                     /locus_tag="PWG71_RS26015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_26015"
                     /product="pyrophosphatase"
                     /protein_id="WP_274812657.1"
                     /transl_table=11
                     /translation="MDFHTLTARVGAVSDRYAERHGIERDSAWFMLKLHEEVGELTEAF
                     LAMSGGTRTKGRTPEEMAAGFRSELADVFCQLLLLADHHDVDLLAEVDAKWSSRLREGT
                     EAPGEG"
     misc_feature    16327..16342
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.56"
                     /tool="antismash"
     misc_feature    complement(16331..16342)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 17.16"
                     /tool="antismash"
     misc_feature    complement(16475..16491)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.57"
                     /tool="antismash"
     misc_feature    complement(16485..16500)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.52"
                     /tool="antismash"
     misc_feature    16597..16612
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.67"
                     /tool="antismash"
     gene            16614..17051
                     /locus_tag="PWG71_RS26020"
                     /old_locus_tag="PWG71_26020"
     CDS             16614..17051
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019607638.1"
                     /locus_tag="PWG71_RS26020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_26020"
                     /product="hypothetical protein"
                     /protein_id="WP_274812658.1"
                     /transl_table=11
                     /translation="MRETAAHAPADTLARPVRPDNTELARALSAQLGLDLAPREFTLAD
                     GTRIGVDCADGDPPGLLVQCSPFAGQMKSSQRNKVIADAFKLSWLRDTHFPRARVLLVL
                     GEPLAKLFGRGAWLPSAFATHRITVALLDPENRVRILDIGT"
     misc_feature    16914..16916
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            17341..18162
                     /locus_tag="PWG71_RS26025"
                     /old_locus_tag="PWG71_26025"
     CDS             17341..18162
                     /EC_number="7.6.2.14"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017603615.1"
                     /locus_tag="PWG71_RS26025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_26025"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_274812659.1"
                     /transl_table=11
                     /translation="MTHPSTDLTDGIRVRGLTKTYAGGDPHRPALESIDLDIGRRRFVS
                     LIGPSGCGKSTLLRVIAGLEDHDAGEVSLFGSTPRALCSAKAIGLVPQAPALLPWRNVR
                     RNITLPRSVNRRAGTGGEDPDDLLRAVGLGDVAHRYPHQLSGGQQQRVAIARAFGMRPE
                     LLLMDEPFSALDEFTREALRLQLLVLWQRRATTVVFVTHSVREAVTLSDEVVVMSARPG
                     RVHEVLPIDLPRPRDASVVTGPRMHALEDRVRASLQSAWEAGAAPDDLVPL"
     gene            18159..19028
                     /locus_tag="PWG71_RS26030"
                     /old_locus_tag="PWG71_26030"
     CDS             18159..19028
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1044: ABC
                     transporter, permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017603614.1"
                     /locus_tag="PWG71_RS26030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_26030"
                     /product="ABC transporter permease"
                     /protein_id="WP_274812660.1"
                     /transl_table=11
                     /translation="MSESIEMTAKAPVRSAPAPRRTTPAPRRRRSTRRGPLHPATWAPT
                     TVLVLVLGLIWSAIATEHPYILPELSEVGLALVDRPEVFLTNAWTTLSIALAGVACGAG
                     TAFVLALVMSEVPVVRRALMPLAVVLNVTPVVALAPGLVLAFGFGMTPKVVVTAIITFF
                     PVLINVTTGLRAVPPSVLHVFETLHASRAEVLWRARILYGLPYTFAALRVVMPLSIVGA
                     VVAEFVAAGSTGGLGTMIRNAASTARLESVYAAIACLAAMGVLMLALVTAVERRVLFWH
                     ESQRRAST"
ORIGIN
        1 ctcaccctgg agtggagctc cggccgtgtg gaaggcaacg tgtgtcggat caaggcgctc
       61 aagcggcaga tgttcggccg cgccaagctc gacctcctgc gcaaacgcgt cctgctcgcg
      121 gcttgaggag atcgcttcac ggagaatgcg ccagagccaa aacacactgg ccattgacaa
      181 cccaggcctg cgacctcaaa aacggcctct gaactggggt tttgtgggta ggcccccagg
      241 ggctcgaacc ctgaacccac ggattaaaag tcggcgggat ggtgggcgtt tcgtcgaact
      301 atgtggtgct tcgttctgcc ctgtccgggc cccgtgatgt caccggagac cacggaagac
      361 caccgcagac cagggggttc cggaacaccg gagccacaac ggagccaatt ctggttcttc
      421 aggagtaccg tcaaaatccg ctggctactc gaggtgggag cgcatatctg ctcggtagct
      481 ctttgcgtgt gttggtctcg cctccgaggc cgacctgttc tgcgacccga tccggtcgtt
      541 ccttccctgc gtgtgcgggt ctcacccagg tcggggaggt gttcgagtag gccgttgatc
      601 gcttcggtcg ggccgttgga tgtgccgggt ctgtcgaagg gtccgtgatc acggctgatg
      661 gcggaaactg atgcgcatca gccgactgtc ggcccagtct tcgagccgag tcggccggat
      721 ggtgccgggg tcgtggggct gattgagatc cgcgacgatt tcggccagca ccgcctgacg
      781 ttcagccggg tcggtgacag gcgtggcccg tgcaggcaga tctgcgcgga tcccgttctt
      841 gaggtggaag gtgaagtcgg gattggcgag caggttcgcg tgccagtcgg tcgcggcacc
      901 gccggcgccg ctacacaggt aggtggtgcc cgcagctcgg tagaagaaga tctcgatgcg
      961 gcgtggccgg ctggtgcggc ggcctagcgt cgtgatgtcg atggtccgtt ctctggtgcc
     1021 cgcggcgggg gtgatctcga tggcttgtcg gatgtggttg ggcaggtggg acagggacga
     1081 gtcccgtgcg gagtcgttcg acccggtcat gtctggagtg ctcatacggt ctcgctctga
     1141 agggcggggc cgaggaggag tccaccgtcg atgacgtact ccgaccccgt gatgaacgac
     1201 gcctctgatg aggtgaggaa caggagaaga cggctgacgt cggtcggctc cccaagtcgg
     1261 gggatggcga acggctcggg tgagtagaag tcggtgattg ccgcggtcgc gccgacagct
     1321 ggtttgtgga tgaagggggt cgagatcacg ccggggtgga tggtgttcac ccggatgtgg
     1381 tcgcggccga gctcgagcgc tgccgtttgg gtgaggcctc gcacagccca cttgctggcg
     1441 acgtagggcg cgtagtgcgc cgtgccgccc aggcccatgg ctgaggcgat gttgacgatg
     1501 gcccctccgg ctgcgcggcg cagagccggg actgcgacct tgatgccgag gaagatcccc
     1561 gtgaggttga tatcgaggat ccgcgaccac gtggcctggt ccgtgtgttc gatcagtgcg
     1621 ggcgggttct gcacgcccgc gttgttgacg agcacattca gggcgccgaa ggcgctctcg
     1681 gcggctagca cagcggcggc ccacgagctc tggtcggtaa cgtcgaggcg gacgaagcga
     1741 gcgcgggttc cgagctcgtc ggccagagcg gcgccgcgtt ccgcgtcgat gtcgccgatc
     1801 accacgttcg ctccctccgc gtgaaaggcg cgcacgtgac tcgctccttg gccgccggtc
     1861 ccaccggtga cgagcacggt ctggttgtcg aagcggggca tcagatgttc cttacgtggg
     1921 tgaatgaccg ggatcaccag tgatgaaccg acggccagtg gtgagtcgtt tgactcacca
     1981 tcgctctgac actaggtcgg cgacagtggc gagtcaagcc actcacctcg tatgctttgc
     2041 ccatgagcag agccgtgacg acgtatcacc agcgcgtcgc ccaagagaag cgcgcgctga
     2101 tcgtgacggc cgcgaccgca ctgtttctcg agttgggcta cgaccggacg tcgctggcgc
     2161 ggatcgcaga gtcctcgggc gtttcgcggg ccaccttgtt caagcagttt ccgagcaaag
     2221 ccgccctgtt cgacgccatc gtgaccgagt cctggtcgac cgctgacgag gaggatcctc
     2281 cgccagcagg caacgtcgtt gacgggctca gcatcatcgg tcgccgttac gccgagttgt
     2341 taggccgcgc ccagatgacc gacctgttca ggatcgtcat cgccgagctg ccgcggttcc
     2401 ccgagctggc ccatgcgcag ttctcgcacg ggaagctgcc ctacttcgag tccctacgca
     2461 gctacctcca ggctgagcac gaggcgggaa cggtgcggat cgaagacgtg gacctcgcag
     2521 ctacccagtt cctgggcatg atctccaact acgtcttctg gccgacactt ctagtgccgg
     2581 actgggaggt gagcgccgaa cgcaccgctc aggtagtcga cgaggccgtc cgcaccatcg
     2641 ccgcccggta cgccgcgaat gggccagggc catcgaccaa cagctgagtc ccaccaccac
     2701 aaggagctga tgcctaagca atcggggccg tcggcaccaa ggacgttcgc gcggcacccc
     2761 gggacggacg ccgaaggagg ggagtgccga tgcgttgacc cacctcgatc ggccggttac
     2821 cggacccctc tgtgtcagac tgtggcggcg accctgcggc gagcgagggt cctccagctg
     2881 ggcgagcttg tcggcaaggc cgacagggac aggggcgggc atggcagact gggggcgcaa
     2941 cggagtccct tggaagtcag agatgtggaa gtccctgatc ttctagcggg ctccgttgct
     3001 cgttggtggg cgcttcgacc ggatcgctac aaggctgtga tcagcgccga tggctcacga
     3061 tcccgacttt gaaacagacc tgggatggga tgcctcacgg gtccggcatg aagggaaccg
     3121 ctgtgaagcg acggacgctg atcggggtca tcggggtcgg gttggcggtt ctacctgtcg
     3181 gatgggctct gcgccaggcg gtgaacggga ccaaggagga ctggcgtacc gacaccgcac
     3241 ctttggagcg ggcgttcccc ggtctgggtt cgctcaccga cgccaaatgg gtgagcaccc
     3301 gggacaacga tcgtctcctt cagacgccgg atctggtgtt cgcaggcttc gcccggctgt
     3361 ccccggggaa actcgatgaa ctgacgacgt ctcacgcctt cgtgtccgag gaaccgacct
     3421 tcgacttctc ctctgggttc acgaaaccgc ttgcggatga gggcccggac gacccacagt
     3481 ggatccggtc acccgaactc gaccgcagca gcgacaacca ctccaccagc ctgtggttcg
     3541 accgccgcag tgacaccgtc cgcttttggg cgagcaaccc gtacggctag tactccagcc
     3601 gcacttggag atctcggtct gagcaacccc cggacatgag acggccaccg ctgtgatcat
     3661 gtgggtgtgt tggaaccaaa cgatcggcgg tggccgtgcc gcccactgta gtagaggccg
     3721 accccgacgc gtgggcgaac gagttcgacg acctgttctc ccaggtcgta gcgcccttct
     3781 tcggccgccg ggaaccccgg ttgcgcgccc gctcctacct gctgggcctg ctcagctccc
     3841 tggagcgcaa gaacggctgg tccctggccg agtacgccag cgatgcgaca ccggacggga
     3901 tgcagcggct gctcaaccac gcccgctggg acgccgaaca ggtccgcgac gccctgcgcg
     3961 cccaggtggg agaacgcctc ggccacctgg acggggtgct ggtcgtggac gacaccgggt
     4021 tcgagaagaa aggacgccgc tcagccgggg tacagcgtca gtacaccggt accgcgggca
     4081 agatcaccaa ctgccagatc ggggtgttct gctcctatgt cgaccccgac cggcagcggg
     4141 tgctcatcga ccgggagctg tacctgcccc ggtcctggtt caccgacccc gaccgactca
     4201 ccgacgcagg agtccccgaa cgcgcgcact tcgcgaccaa accggagctg gcctgggcca
     4261 tgatcgagcg ggccgccgac gacccgttgc tgtcgttcgc ctggatcacc gccgatgagg
     4321 cctacggcga caacacccgc ctgcgtgagc gctgccgtga ccgggggctg agccacgtca
     4381 tggccgtctc ccgtgaccac cgcctggtcc tgggcggggt caggaccagg gccgacaccg
     4441 ttgtctcgct gctggcccct gatgcgtggc agcgctatgc ctgcggggac ggtgccaagg
     4501 gccgccggtt ctacgactgg gcgtggatcg gcctgggcgg gcacgcgtgg gtgctggccc
     4561 gccgctcgat cagcgacccc acggacctgg cgttctaccg gtgctggtcg gtgcggccgg
     4621 tcgccttgtc ggagctggtg cgggtggccg gggcccggtg gagtgtggaa gaggtgttcc
     4681 agacgaccaa gaacgaggtg gggttggatc actaccaggt ccgcacgcac accgcctggt
     4741 accggcacat cacgctggcg atggtggccc acgcccacct ggccttcctc gccgccgatc
     4801 cgccacccgg tcgggagggt ccggtcggcg aggaaaccga tggcctcgcc acacaggggg
     4861 cccggcccgt ggggacgatc gccgcgtgag gggccgggcg gtgacgatcc gggcgagctg
     4921 atcgggctga ccgtcaacga gatccgccgc ctgcacgccc actaccaccg acccgatcat
     4981 gcctggtcac accgggtccg ctggtcgcgg tggagacgcc gccatcaggc gcgggctcgt
     5041 cgctgccact accagcggca aagacgcctg atcgatcact aagtgcggct ggagtactag
     5101 aagaagccat cgtggggcct cgccaatggc cgaccacggc tgcccactcg cacgcaggga
     5161 cggcaagcgg cggtgacgct agtaccgtgc tggcctaggg tgccggtgtg agcacccaat
     5221 ggacgatcca cggtgagaag ctcgtggacc agaaccggca catccgtctc tccaccgtcg
     5281 atatcaccct ccccgacggg gtgtccttca cccagtacgt cgttgccatg ccgccagccg
     5341 cgatgaccct ggtagtgaac gagcagcgcg aagtcctgtt gatgcggcgg caccgattca
     5401 tcatcgaccg gtgggtgtgg gagctgcccg gcggctacgt cgacggcgtc gagaacatcg
     5461 aagcagcggc ggcgcgtgag gtcgaggaag agacgggatg gcggcctcgc tcgatcgaac
     5521 acctggtgac cttccagcca gccatcgggt cggtcgacca accccagatc atcttcctgg
     5581 cccgaggcgc cgacctcacc gacacggcgc cggacatcaa cgaggcccag gaagtccgtt
     5641 ggtggccgtt ggaggaagcg gtggaaatga tcgcccgagg ggagatcatc ggtgcctcta
     5701 ccgtggtcgc cttgtatcgg gctctcacct tggtgtgagt tcctcggccc gcgtcgtgaa
     5761 ctcctccacc gtgggcagac cctcatgggg gcggagtcgg tgccgaaggc gacgcaggta
     5821 ggacagagcg cgttgtgact gtaacccctc caccaaatcc agggccatcc cgccgacccg
     5881 taccgcctcc tctggttcgt cagtagcgcg ggccgtggcg agcatcaaga gattgaacgt
     5941 cttgccgcgc acatagtcgg tgttcatctt cagagactgt gccgcgtatg cggcggtctg
     6001 agagtcgttg ccgagggcga ggaaacaatg tgcgaccttg gccgcgaggt aggcatggtc
     6061 gaaataggcg agccactccg gcgggtcagg ggcatccgcc tcgaacgcct tcacccctgc
     6121 ccgcagggat cgggacgtgg cacgcgggtc cgagagtccc gcgtgcccgt gtgcttcgat
     6181 gaggtggcat tccgccgcga gtgcgggatg ccctgaccgg tgagcggcgg actgtgctgc
     6241 tctcgccagg tccacggcct cagccgaccg accaatgtag gtggcttggt gccccatggc
     6301 cgcgaggatc tcggcacata aaccgtcgtc accggctgtc tgggccattc tcagagcttg
     6361 gatcagatac cgctgggcaa ggccgtgttc ctcacagtcg tacgccatcc agccgacggt
     6421 cttcgtgagt tcggcagccg cagagaacaa atccctaccg acttcttcct tgtaagtacc
     6481 gcgcagcaaa ggagcgacgt tggcgtcaag gtactgcaca acagtgcttc ggatacggcc
     6541 tccaccgacg cgattgtcca ggtcacggaa agcgcgtgtc atctgccgga tggccgagat
     6601 ctctggacgt ccgacacgga tccggccacg gtgggatggg ccagccgtag aaggctgggt
     6661 gagccaccgc acggtcgctt tgggataagc cgccgtagag tacccactga gaagatcgcg
     6721 ccgatgctcc ccgtcatcgc ggtaaagctg gctggccgaa ctgattcccg cagtccagga
     6781 gagggtgaac tcgagagttc ggctttcgtc ggtgggaagc ccacaatcct caggaagcac
     6841 cggacggccc aacgcctcag aaaggactcc agcgatgatc gcaggggtcg gccgctgtgg
     6901 ccgctccccc gacaaccacc ggcggacact gttgtgatcg taggagaagg ccagcccctg
     6961 ctccttaccc cgggccacca ctcttcgagc gagccctttg gctggcattc cggcctcgtc
     7021 caagagttct gagagctggg tgtttggttt ttttggagca cgcgccatag ctttccccac
     7081 tggtccgggt caaaagcagt gtggcacgcc tgccttccca gaggcggaga ttcagagaat
     7141 cgcactccca tcctgcactc ctgtgtctcg gaagcgcgct catctccctg caacaagcct
     7201 ccacgaaagc tgccaagtaa cagaacgtag aaccgtcgat cgccacgtag gcggaaggtt
     7261 cacaaaagtg gagaagcgag tgcggatgga caccccagag gcggaggccc gagagctctc
     7321 cgaagaggtg ctggtggagc tcggcgacgc cgccgacctg acgctcggcc agggccgggc
     7381 ccagaacgag aacaagcgca acccctacaa ctgactcgcc gaggcgtcca cggccgttgc
     7441 ctccgcgcgg cggacgtgga cgctctcccg aacggataag gatcgccatg tggttcggtg
     7501 gttccaccgg ccccattccc ctacgtcctg cgaacgccgt cccggtgagc ccgaacgtcc
     7561 ccggggtgtg gatgagcacc gagtggcccg agcgcgaact cgtcaccgct gcgcatggtg
     7621 aacggcatgt cctggtgttc ggcacctgtg ccgtcgagtc cgccgagctt caccgggcca
     7681 cggcgccggc cgtccccggc gatgtggcct ggcggtggcc gggaagctat gtggtggtcg
     7741 aggtgacccc cgggtccgtc actctgtgga cggatctgac ctcggcactg cctctctaca
     7801 tccgcagaac cgaggacagg gtgttgtggg cttcgagttc ccggctcctg gccgggctgt
     7861 acggctctgt tgaggtcgac ctcgccaagg tggtggagcg gctgatcggc cccgacacgg
     7921 cagagcatgg caccggttcg tacttcaccg gggccgagag ggttcccgcc gggcaccgac
     7981 tcgtcctcag caaaggcact gaacctgtgg cagggcgggt gtgggaaccc aaggatgtcg
     8041 gctccggtca tggagagcgg ttgcggtcgg cgctggaagg ggccgtggcg gttcgtgtgg
     8101 accgcgccgc gaggcccagc acggacttct ccggtggctt cgactccacg gcactcggcc
     8161 tcctggccgc cgaacgactg gcgccgcgcg gccgagagat cgtcggggtg accgtgcacc
     8221 ctgacggtgt actctacggc ggcgacctgg actacgcccg cgaagcggcg cagcatcccg
     8281 cagtgctcca ccgcctcctc cccttgggtc gggagcattc cccctatggc ggcctggact
     8341 gtgttccacc caccgacgaa cccgcaccgt cgacggtctc gtacgcctac ttctccgccc
     8401 agttgcggtg gctgtcgcag gaagtcgact cggacatgca catgaccggc gatggcggag
     8461 acgccctgct actgactccc ccgtaccaac tggtggaact gctgcaccga ggacgggtgc
     8521 ggcacgcttt ccaggaggcc gtccggtggg cccacgtccg caggatgtcg gtcctcaacg
     8581 ccttgcgggc agcccgttcc agggcggacc ccgatcccgt accgacctgg gtacgaatca
     8641 cctcgcccgt accgcagggc gtcgaggatg cctcgccccg gggacgaaca cgccggatgc
     8701 tggagcggac catgatccac gccgggcgca ccgcgcgagc ggatgtgcag ctggcccagg
     8761 ctgccggggt ccccctgcac aacccctact tcgactccca ggtggtgaac gcctacctga
     8821 gcgtccccat ggaggacctg cccggcccgg ctcgctacaa gccggtcatg gccgacgcga
     8881 tgcgggacct gttccccgca aagctgttgg cgcgcaccac caagggcgac gcctccagtg
     8941 accaccacga ggggctgcgg cgatccctgc ccttcctgat ggagttcgtg gacggccacc
     9001 tggcccagga ggggctgatc aacgtgtccg ctctacgtaa ctcgctgtcc cgtgccgcac
     9061 tgggcatcgg tgaggagctg gcgcagatcg agtccacggt ggcgaccgag gcatggttgc
     9121 gcacagcaca ccgcgctcca gcccccgaat ggttcccgga caaggcggtt ttgcaatgac
     9181 tccggctact gaagaacgtg cctgcgacct cggctccgca acggtggtcg tccactacag
     9241 gacagggacg gtgcgggtcc tttccccagc ggctgcccgg aagtggagaa cagatcgcga
     9301 ttccgcggcg atggtggccg gccatcggtg ggaaccgagc tggggcgtac aggagctgcc
     9361 catgggcttc tcggaattcc ccagcgtccc cgcgcggtac cggatcgccg caaccgtggc
     9421 cctgcccgtc tccctggccg tcatgcgggc cggaccgcgc tcggctcgga tgggccgcat
     9481 ggtgcgtctg gtccgggcat ggcgcggcgg cggccggatg gccacgcttc cagaggcgga
     9541 gaacgccgtc catgccgtac ggcggctcgg cctgttctcg cccgtgcggg tcgcctgctt
     9601 ggaagaatcg gtcgccgtgg tggtggcact cgccctgctc ggcaagcgag tgcgctggca
     9661 ccacggagtg atcaccgacc ccatccgcct gcacgcctgg gtcgaggccg aaggcctgcc
     9721 cgtggccgaa ccggagagca ctcgcaggtg cacggccctg ttgaccatca cccccacctc
     9781 ggaggagaac acgtgagtga accgttgatc tggctgcgtg gcgacagggc cgggatcggc
     9841 ccgttacgcg ccgatctcgt ggaggagtac tggcggtggg agcagtcgat cccgacgatc
     9901 gtcggctaca accggcagac accacagccg gtggagagca cccgggacat ccttctggag
     9961 ggctacgcgc gggcatcgga ccgggagttg cggttcaccg tctacggcct gaccgggcag
    10021 gagcctcgcc ccgtgggcct ggcccaggtg tacatcgacc agatgcgccg caacggcgag
    10081 tacgtggtgg ccctgggcga gtcccgcggc aagggtgtgg gaacggaggc cacccgcctg
    10141 gtgctggact acgccttcca cgtcaccaat ctgcggtgcg tgtacctcac cgtcctcgcc
    10201 ccgaacaccg gagccatccg tgcctatgag aaggccgggt tcaagcgcca gggcgtgcgg
    10261 cgcaactcca accagtggct cggcgagacc gtggacgagg tgctcatgga cgcgatcccc
    10321 gaggagttcg agggatcctc actggtcaaa gcccagttcc aacggtgaac gcccggtgcc
    10381 cgccggcctc ggcgggcacc agcggtcagt cggtgttcag gccaccgagg aagtgcgccc
    10441 acgagcccgg ggtgaagtcc agcggctgca cgtggaggtt ctggttgtcg cgcacgcgcg
    10501 tcaccgggcc ttcggagacc tccacgcagg tcccggcctc gttgctgtaa ctggacttgt
    10561 gccagggcgt gctctgggtg ctcatgtggg gaaccttcct cagtcgagtt cggcgatccg
    10621 ggaccggatc aggtcgcggg acctctcggg ggacagcgcc gccgcccgga ggtggtggga
    10681 cctcagctca tggtgttgca cgtcctccgg ggcgtcgaac agcagcccct ggggaggcac
    10741 gtccacgtgg ccgacctggt cgtccccgag ccagagcagg ctgaagccgt agttgaggct
    10801 catgccctcg gcctgcggca ggatctggag gtcgatcctg gggtggtcgc tcagctccag
    10861 gaggtgtgtc agttgggcgc gcatcgtgcc ggggccgccg acctggcagg tgaggacggg
    10921 ttcgtcgatc acccaccaca tctgcggcgg ttcgtcgcgg tgcaggatgc tgtgggcgcg
    10981 ctccaggcgg gcttgggtcc tgcgctcgcg ctcggcctcg tcgggaacgg cgccccgacc
    11041 catgagagcg cggatgtagt cggggtgctg ggccaggccg ccgaacaggc cgatcgtgta
    11101 ctcgaagatc tgcccggcct cgtactccag cgggtagtag gcgccgccgg ggacatcctc
    11161 gtaggcgttc caccaggcgg aggtgcggtt gcggctggcg gccttggcga tgtgggccaa
    11221 ttcctcacgg agatcgtcgg ccgctttgta gaggcggcag agagcgtcga tctccgggct
    11281 gctcagacgc ttccacttgc cgttctccat gcgggagacc ttggacttgt gccagcccat
    11341 ctcctcggcg gcctcttcct gggacaggcc ggtctgcacc cgtagccgct tgagctggct
    11401 gaccaggtag tgcctgcggg cgggggaacc gtgcgcggga gtgccctcgg gcatctccac
    11461 ctcctgtccg tcgcgatacc ggcgctgtca tcttcccacc caggggaagc acgcattatc
    11521 actcattgta cgcaaatcaa cttgctgcgc ttgtaatttg cgttgttgca aatcatgatg
    11581 gatgcacagc gggccggaag tgagcggcga cgcggcccgc ttcgcatcgg tgacgacagc
    11641 gaggtgatgt gcggtgcacg aagtcatgtg cgtctggagg cgcccgggac cggaggacgt
    11701 gggtctggcc cggtgtctca cggacggggc actggcggcg gcaggggtgg acgaggcagg
    11761 gctgggcgtg gcccggctgg cggtgagtga ggcgttcacg aacgccctgc tgcacggccg
    11821 ccccgaggtc ggtgtggagg tctgggcgcg accgggccgc tgcggggtgg tcgaggtgta
    11881 cgacaccggg ccgaccgtgc cggtgttccc ccagccggag gagctggcct gggtgaccgg
    11941 cgaccacgga cgcgggctcg ggctgatccg ggcgttcacg cgcgaggcgt gcggggccta
    12001 cgcctgcggt gtgggcaagc gggtgtggtt cgcggtggcg atggacggcc gtgccctgga
    12061 cgtgcaccgc acccgcgcac tggtggaggg acgcgcccgc ctgcgggcac cgcgctccac
    12121 ggcgctcctg gcgtgaacac ccccgcgacc ctgaaaacga agcgccccgg ctggtgctgg
    12181 cacaccggcc ggggcgtgat ccccacctga caccagcagg aggaaatcat gcgtcaccct
    12241 actaccggcc gtcaccgtcg gccctgcgac agcatggccg ggcgcgtgtg ggccctgacc
    12301 gcagcgctac tggccacagc cctggccacg atcttcagtc cacggctgga ccgtcccacc
    12361 caagtacccc gcgcattgcc cgcagcgccg acgcccggcg gcaaccccgg gcacgttaag
    12421 caaggccgtg acctgctctc cgacccgccc ggcttcccgg agcagagcgt ctgggcggcc
    12481 ggggcgtccg catccccgac tcgctgggag caggcctacc gcgaccgctc aacaccgttc
    12541 gcaggggagg atgtggtgga cgccgacccc gaccagatcg ccggagccct ggtacgcccc
    12601 tacctcgacc ttgccgcggt tccccgaccg cgctcggccg agccgagcga ctacccggcc
    12661 aggtcctcgg ctgtggagtc cgaaccggtg gaggacgagt tcgccgagct ggccgaccgc
    12721 attcgcactt acctggccat aaggaactga aacgctattt atcgggggtg ggcctgtggc
    12781 agggcctgtg actgggcctt ccagagggcc tgtgaccggg ccgataggtg ggcccattag
    12841 tacggcccgc cccgtgcgcg ttatcagtgc aggtagaagc cgcttcgggt gggcccttcg
    12901 caatccagac acatacccaa gtgggcagtt cctcttcacg tagggcgggg cggcgaccaa
    12961 gggcgaaggc cccgtccagc cctctcagac gccaacgaca gcggttcggg ctggggcgat
    13021 gtgcggcatt cagcgggcct gcacgtccac agcacgtacc aggaccacca acgggtgttc
    13081 agcggcgcag cgggcgctgg tggacgtacg tgggctcgtg tccggggccg gggcgggaac
    13141 ggcgtgacgg cgccccggcg gaagagcgcg gagcgaggat gctcgggagg tgggaggcac
    13201 ggtcaagaca aaagagcgag ctccgagcaa tcagcgggca cctggaacga gaagaaccca
    13261 ggaccgagac ctcaaaaatg gcctctgacc tggggttttg tgggtaggcc cccaggggct
    13321 cgaaccctga acccacggat taaaagtccg ttgctctgcc aattgagcta gaggcccgtg
    13381 accgacccgc gtgagcaggg ctgacgccac cgaggccaga gtctaccgga taccactgcg
    13441 ggcacacagc cgagtatcca ccgcccgcga acacgcgccc tccctacgcc accggtcttc
    13501 cgcgctcccg accgcttcgg cgcgccgtcg agtgggcccc tgacctcggt cggggcccac
    13561 cgggtcctat cccacgagca cgatcgcctt ggtgtgcagc ttgtcgcgct ccaggaggga
    13621 ctgggccgaa tcctcgggcc agaacagcac gaaccggccc gcctggcggg cgtcgccggt
    13681 ctccgttccg gccgcccgcc gggcagcgga gtccgcgtcg ggcatctcgt acaccgacac
    13741 gagatcggtc gtgaccaggg cgaggcagcc gtcatcctcc gaggcgcacg acgacgactc
    13801 gtcgcgcgcg tcggggagcg ggtacagcgt gtccagttcg gcgacgaggg tctgggccgt
    13861 gtacccgtcc gtgggttccg ccggcacctc ttccgaggag acgccgggag agggggcgag
    13921 gtcctggccg agttcgcggg cgaggaggac gccgccgatc gtgagaacgg atccggcgac
    13981 gaccagaccg aggcagccga tgccgagacc gagggccacc tgggttccgg tggacccggc
    14041 cttgccggga gaaggggcgc cgtgggaggg aggggggtcg tgggacacgg tggggctccg
    14101 aggggtggga gcggcggcgc gggtgccgcg ggcgctcgat atggggggtg agcgtagccg
    14161 aggaggcgcg gggacgagaa ggcgcgggaa cacggtccgc ccctccgggg aacacggccg
    14221 gcggcccctg ccccggccgg gctcccctcg gcggccgtac cgcggagcga tcatggaggg
    14281 caccggccgg aaggccgtcg tgacgcgccc cttgaccctg acacggtgtc aggcccgaac
    14341 gtggaggtgt catgttcacc atcggagagt tcgcgtcgat cggccgggtg agcgtcagga
    14401 tgctccggca ctacgaccgg atcgggctgc tcacacccgc ccgggtcgac cccgtgaccg
    14461 ggtaccggtc ctacgaggtc gggcagctga cccggctgag ccgcatcgtc cagctgcgcg
    14521 gtttcgggct gggtctggag gacatcgcgc gcatcgtgtg cgacgacccc gatcccggga
    14581 gggagcggga gatcctccgg gccgcgagcg cggagctgcg gcgggacatc gtggagcgcc
    14641 gcgcccgcct ggaccggatc gacgcctatc tccgacgcag ggaaggactg cccgccatgt
    14701 ccacatcgac caccatcacc gccgaggtcc ggtgggtcgg gccacagcgg atcgccacgc
    14761 tgacgcgcga gatccccggc ccgggccccg agaacgtcgg ccccgtgctc ggccccctct
    14821 atcccgaggt ggcgggggtc ctggcggcag ccgggatcga cgtcggtccc ggcatcgcct
    14881 tctaccggta cgacgagtcc gaggaggcga ccgggatgac cgtgaccgcc ggcttcgtgg
    14941 tccccgacgg ggtcgacgcg gcccccgggc tggaggtctc caccctgccg ggctgcgagg
    15001 cggccgtgac cacccaccgg ggcgaggtcg ccgggatcgg ggagacctgg gccgcgctgg
    15061 tcgactggac gcgcgagcac ggcctggggc tcgccggcgt ctgccgcgag gtgtactgga
    15121 cgcccggcga ccgcccccaa gaggagtggg tgaccgatct ggtgcagccg gtgcgacccg
    15181 ccgggtgaaa aaccgctggg gcggagcggg cgcgtgtgtc aggctgcccc gcatgagcga
    15241 tgtccgtacc gacctgttgc gccgccagtt cgacctgacc tgggggctgc tggagtacca
    15301 cctggaccgg ctggtccccg aggacgtcct ctgggaaccg gccggcctct gctggacgat
    15361 gcgcccggag ggcgacggtt ggtcccccga ttgggccgac accgagcccg atcccgtgcc
    15421 ggtccccacc atcggttggc tgagctggca catcggctgg tggtgggagg tcaccctcga
    15481 ccacctggcc ggccgggtcc cccgggaccg ggaggccgtg gactggccgg gtccggaggg
    15541 tgtggtggcg cggctggtcg gcctgcggga ggagtggacg cgggtgctgc ccaccgtcga
    15601 cctggacgcg cccgccccct tcccgtggcc ggaggggtcc gggatgaccg tggcggacac
    15661 cgcggcgtgg gtcaacgccg aactgatgaa gaacgccgcc gagatgggtc agctgcggat
    15721 gttgcgtgcc gtctcctcgc cgggatcgta gacgcggccg agcgtcccgg gaggctccgg
    15781 ggaggcgcgc cgcccggcct cggggggagg gcgcgcgccc cggtgtgaag aacggtgcgg
    15841 acggtgcgcg ttccggccgg gtgaagaacg tcgttccttc cgcgatcccc ggacgcggtt
    15901 ccggtccgtc gtgacgggcc gttacgatcc gttccctcga agagcgtgat ctcagggcaa
    15961 ggggcggatc catggacttt cacactctca ccgcgcgggt cggcgcggtg tccgaccgtt
    16021 acgcggagcg gcacggcatc gagcgggact ccgcgtggtt catgctcaag ctgcacgagg
    16081 aggtcggcga gttgaccgag gcgttcctgg ccatgtcggg cgggacgcgc accaagggcc
    16141 gtaccccgga ggagatggcg gcggggttcc ggtccgaact ggcggacgtg ttctgtcagc
    16201 tgctgctcct ggccgaccac catgacgtgg atctgctcgc ggaggtcgac gcgaagtggt
    16261 cgtcccgcct ccgggaaggc accgaagccc ccggtgaagg ttgaactccg acttctcgga
    16321 taccgaaagg aaaaaccttt tctcttccgc cttccgactt tctccgataa ccccggggga
    16381 ccatcgggag gacgacgtgg agcacgtggg cgtacggagc gcgtggagcc cgaaccccct
    16441 tctggtacgg ggttccagtg taaacatccc acctttgcgg ttcgtgtgaa actcgacaca
    16501 aaaataatcc gccaaaatcc caggtgagca cagacgaccc gtgccaggac ccgcgcgaca
    16561 aaggccgagt gacgcgaccc ttgggacact tgtgcactaa agtcttcttc accatgaggg
    16621 aaacagcggc acacgcgccc gcggacaccc tcgcccggcc ggtccgaccc gacaacaccg
    16681 agctggcccg agccctgtcc gcgcaactcg gtctggacct ggcgccgcgt gagttcacgc
    16741 tcgcggacgg cacccggatc ggcgtcgact gcgccgacgg ggatccaccg ggtctgctcg
    16801 tccagtgttc gcccttcgcc ggtcagatga agtcgtcaca gcgcaacaag gtcatcgccg
    16861 acgccttcaa gctgtcctgg ctgcgcgaca cccacttccc ccgagcccgg gtgttactgg
    16921 tcctgggcga acccctggcc aagctgttcg gtcgcggcgc ctggctgccc agcgcgttcg
    16981 ccacccaccg gatcacggtc gcgctcctgg acccggagaa ccgtgtgcgc atactcgata
    17041 tcggtacgtg acggagcctt catgcagagt taatcgaagc tccttagcgt ctcacctcgt
    17101 cacatgaacc ccgcgggccg tcgatcccct cttccacccg ctctccatcg cctgtccccg
    17161 gtccgtcgtg cccggggacc gacacccgga gggtgcctcc cgtggacttc ccccttacga
    17221 cggccggtgt caccggcccc cgcccccgct cccccctcgg ttgatcgcct tgcgcaccat
    17281 cgacgtgccc tcgatcgcga ccgtgtgcga ccatgtgcgc acagagcatg aggagcgggc
    17341 atgacacacc cctcgaccga cctcaccgac ggcatccgcg tacgcggcct caccaagacc
    17401 tacgcgggcg gtgatcccca ccgtccggcc ttggagtcca tcgacctcga catcggacgg
    17461 cgccgcttcg tcagcctcat cggccccagc ggctgtggga aatccaccct gctcagggtg
    17521 atcgcgggac tggaggacca cgacgccggc gaggtctcac tcttcggttc gaccccccgc
    17581 gccctgtgct cggccaaggc gatcggcctg gtcccccagg ccccggccct gctgccctgg
    17641 cggaacgtgc gtcgcaacat caccctgccc cgcagcgtca accgccgcgc cggcaccggc
    17701 ggggaggacc ccgacgacct gctgcgggcg gtcggcctgg gcgacgtcgc ccaccggtac
    17761 ccgcaccaac tctccggcgg gcagcaacag cgagtggcca tcgcccgcgc cttcggcatg
    17821 cgccccgagc tcctgctcat ggacgaaccc ttctccgccc tggacgagtt cacccgggag
    17881 gcgctgcggc tgcaactcct ggtcctgtgg cagcgccggg cgacgacggt cgtcttcgtc
    17941 acccactcgg tgcgcgaggc ggtcaccctc tccgacgagg tcgtcgtcat gtcggcccgc
    18001 cccggtcgcg tccacgaggt gctgccgatc gatctgcccc gcccccggga cgcctccgtg
    18061 gtgaccgggc cgcgcatgca cgcactggag gaccgcgtcc gcgcgtccct ccaatccgcc
    18121 tgggaggccg gtgccgcccc cgacgacctc gtaccgctgt gagcgagagc atcgagatga
    18181 ccgccaaagc ccccgtccgg tccgcacccg ccccacggcg gacgaccccc gccccgcgac
    18241 gacgacgatc gacccgtcgc ggaccgctgc accccgccac ctgggcgccc accacggtcc
    18301 tggtcctggt gctggggctg atctggtcgg cgatcgccac cgagcacccc tacatccttc
    18361 ccgaactctc cgaggtcggg ctggccctgg tggaccggcc ggaggtgttc ctcaccaacg
    18421 cgtggaccac cctgtcgatc gccctggccg gcgtggcctg cggcgcgggc accgcgttcg
    18481 tcctggcact ggtgatgtcc gaggtccccg tggtccggcg cgcgctcatg ccgctggcgg
    18541 tggtcctcaa cgtgaccccg gtcgtggccc tggcgccagg gctggtgctg gcgttcgggt
    18601 tcggcatgac gcccaaggtc gtcgtcaccg cgatcatcac gttcttcccc gtgctgatca
    18661 acgtcacgac cggcctgcgg gcggtgcccc cctccgtcct gcacgtgttc gagaccctgc
    18721 acgcctcccg cgcggaggtg ctgtggcggg cgcgcatcct ctacggcctg ccctacacgt
    18781 tcgccgcgct gcgcgtggtc atgcccctgt ccatcgtggg cgccgtggtc gcggagttcg
    18841 tggccgcggg cagcaccggc ggcctcggca ccatgatccg caacgcggcc tccacggccc
    18901 gcctcgaatc cgtctacgcc gcgatcgcct gcctcgccgc catgggcgtt ctcatgctgg
    18961 cgctggtcac cgccgtcgag cgacgggtgc tcttctggca cgaatcgcag cggcgtgcct
    19021 ccacctagac ccgcagatcc cacccccaca ccctgtccaa cgaacgaaag aactcccaga
    19081 tgatcacccc caccacatcg ctctcgcggt acctcggtgc cgcggcactg gccacggcgg
    19141 tcctggcgac cgccgcctgc ggcggctccg aggcctccac cgaggaggac cccacgacgg
    19201 cgatggccat cgacgaggag cgctgcgaga ccaaccgcgc ggccgggacc gtcacctaca
    19261 tcaccggtta ctacttccag gcatcggtga gccagttgga ggtgttcgcg gccgaggaac
    19321 tgggcttctt cgacgccgtc tgcctggacg tcgaggtcca ggccggggtc ggcgacatca
    19381 tggccaactc gcagctggtg tcggcgggca ccgcccactt caccccgctg gccaacgagg
    19441 gcgaggtcgt acaggccaac gagaacgacc acgagatcgt cggcctggcc acctacgggc
    19501 acgtgccgat cgcctccctg ctgaccaagc ccgacatcga ggacctcaag gacctggagg
    19561 gcaagaccct gggccgcccg ggcatgctgg gcgccccgct ggaggcgatg ctgctcgccg
    19621 ccgacgtcga cctggacgcc gtggagcagg tcgagaccgg ctacgacccg agcatcctgc
    19681 cgcgcgacca ggtccaggcg ctggccgcgt accgctccaa cgagcccaac cagttggacg
    19741 ccatgggcga ggagtacacc gagtggctgc ccgaggactt cggggtggtc ggctcc
//
