LOCUS       NZ_JARBUT010000021     10405 bp    DNA     linear   CON 29-APR-2024
DEFINITION  Nocardiopsis sp. N85 Scaffold21, whole genome shotgun sequence.
ACCESSION   NZ_JARBUT010000021
VERSION     NZ_JARBUT010000021.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nocardiopsis sp. N85
  ORGANISM  Nocardiopsis sp. N85
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsaceae; Nocardiopsis.
REFERENCE   1  (bases 1 to 117362)
  AUTHORS   Liu,S.
  TITLE     Nocardiopsis sp. N85
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 117362)
  AUTHORS   Liu,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Microbial Chemistry, Institute of Medicinal
            Biotechnology, Chinese Academy of Medical Sciences, Tiantanxili No.
            1, Beijing 100050, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 1.05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 182.0x
            Sequencing Technology :: combinatorial probe-anchor synthesis
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028882275.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 06:01:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,663
            Genes (coding)                    :: 5,611
            CDSs (with protein)               :: 5,611
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 46 of 53
            Pseudo Genes (internal stop)      :: 0 of 53
            Pseudo Genes (multiple problems)  :: 3 of 53
            CRISPR Arrays                     :: 13
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:47:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 87306
            Orig. end                         :: 97711
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JARBUT010000021.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(70..84)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.74"
                     /tool="antismash"
     misc_feature    complement(71..84)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.03"
                     /tool="antismash"
     misc_feature    complement(202..219)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 20.42"
                     /tool="antismash"
     gene            complement(295..918)
                     /locus_tag="PWG71_RS23725"
                     /old_locus_tag="PWG71_23725"
     CDS             complement(295..918)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017603960.1"
                     /locus_tag="PWG71_RS23725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_23725"
                     /product="copper resistance CopC family protein"
                     /protein_id="WP_274812183.1"
                     /transl_table=11
                     /translation="MTRTHASPLRRALVPAVLVAAALMAAPAPALAHDVLTGSNPKDGA
                     TLDTVPEEVVLSFNNEPMTGGSGSAVVVTGPDGETTYEDGEPAFDGLDVSVGLLPLEEA
                     GEYTIGFRVVSSDGHPIQDSLTFTVTEEAVAAAAPEESAEPGGVAEETAAESAPAEEVE
                     ASEAAAEEEGGLPVGVIVLVVVGVIALAAIAIVAVRLRKGSPTE"
     gene            complement(994..1569)
                     /locus_tag="PWG71_RS23730"
                     /old_locus_tag="PWG71_23730"
     CDS             complement(994..1569)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017616170.1"
                     /locus_tag="PWG71_RS23730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_23730"
                     /product="copper resistance protein"
                     /protein_id="WP_274812184.1"
                     /transl_table=11
                     /translation="MRPAHGTLRLLRTASLAAACTGLAWVGHDPWAGGPAGPGGFVLAT
                     VVLAPVLWHFTRVMRGFGDIFAVMATLQVILHLILQGSADPAFATFDTGVDHSGHGVLA
                     HAVGFAPGMLLAHLWAALLASALLAYGESALWSLAALLSRALPPARARALPLPPTPGIA
                     ATVAPAVPTAPALSAHRPRGPPPIALAA"
     gene            complement(1725..1988)
                     /locus_tag="PWG71_RS23735"
                     /old_locus_tag="PWG71_23735"
     CDS             complement(1725..1988)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017590334.1"
                     /locus_tag="PWG71_RS23735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_23735"
                     /product="WhiB family transcriptional regulator"
                     /protein_id="WP_274812185.1"
                     /transl_table=11
                     /translation="MNRLYHESEDWVTQGSCRAYDPEIFFPVSSTGIGRADTEQALAVC
                     RGCTVRPECLRWALRSGEAHGVWGGTTPEERRYMRRELAPAV"
     misc_feature    complement(2461..2478)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.81"
                     /tool="antismash"
     misc_feature    2663..2676
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 20.3"
                     /tool="antismash"
     misc_feature    complement(2674..2683)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.85"
                     /tool="antismash"
     misc_feature    complement(2740..2758)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.26"
                     /tool="antismash"
     gene            2866..3372
                     /gene="ectA"
                     /locus_tag="PWG71_RS23740"
                     /old_locus_tag="PWG71_23740"
     CDS             2866..3372
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019610152.1"
                     /locus_tag="PWG71_RS23740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_23740"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_274812186.1"
                     /transl_table=11
                     /translation="MRLAHPEPEDGPHLWSLARTTGMDLNSPYAYTLWCRDFAATSVVA
                     RDDTGRPIAYLTGYVRPDSPDTYFLWQVAVDPAHRGRRLARYMLDFIGDRIVERGIEHL
                     EATVTPDNTASRALFASFARDRGARITWTALFSEEHFPKEDGESHEPEDLVRIGPLGLP
                     DEEQA"
     gene            3465..4730
                     /gene="ectB"
                     /locus_tag="PWG71_RS23745"
                     /old_locus_tag="PWG71_23745"
     CDS             3465..4730
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (22-357). E-value: 2.7e-73.
                     Score: 238.8. Matches aSDomain:
                     nrpspksdomains_PWG71_RS23745_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019610153.1"
                     /locus_tag="PWG71_RS23745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_23745"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_274812187.1"
                     /sec_met_domain="Aminotran_3 (E-value: 6.8e-76, bitscore:
                     248.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METFERLESEVRGYCRNWPVVFDRAVGSHVYDETGRPYLDFFAGA
                     GSLNYGHNNPELKSALIRYLTDDRIVHSLDAYSVAKRDFLSTFEEIVLKPRGLDYKVQF
                     PGPAGNNAVEAALKLARKYTGRETIVNFTNGFHGMTLGALAVTGNSMKRGGAGVPLGHV
                     ATMPFDNYMDGRTPDFLWLRSLLEDSGSGLDKPAAVIVESIQGEGGINVAGAQWLRELS
                     ELCREYGILMIVDDIQMGCGRTGDFFSFEEAGIVPDIVTLSKSISGYGLPMALTLFKRE
                     LDVWEPGEHNGTFRGFNPAMVTATEALRRYWTDDAFANATKAKGDLVAARLDEMAAEHA
                     EFGAHVRGRGLARGLAFTDTDLAKKVAAESFDRGLLLETSGPEDEVAKLLPPLTMTDEE
                     LTAGLDIMADAARAAAKVAQPA"
     aSDomain        3531..4535
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PWG71_RS23745_Aminotran_3.1"
                     /evalue="2.70E-73"
                     /label="PWG71_RS23745_Aminotran_3.1"
                     /locus_tag="PWG71_RS23745"
                     /protein_end="357"
                     /protein_start="22"
                     /score="238.8"
                     /tool="antismash"
                     /translation="DRAVGSHVYDETGRPYLDFFAGAGSLNYGHNNPELKSALIRYLTD
                     DRIVHSLDAYSVAKRDFLSTFEEIVLKPRGLDYKVQFPGPAGNNAVEAALKLARKYTGR
                     ETIVNFTNGFHGMTLGALAVTGNSMKRGGAGVPLGHVATMPFDNYMDGRTPDFLWLRSL
                     LEDSGSGLDKPAAVIVESIQGEGGINVAGAQWLRELSELCREYGILMIVDDIQMGCGRT
                     GDFFSFEEAGIVPDIVTLSKSISGYGLPMALTLFKRELDVWEPGEHNGTFRGFNPAMVT
                     ATEALRRYWTDDAFANATKAKGDLVAARLDEMAAEHAEFGAHVRGRGLARGLAF"
     misc_feature    4930..4944
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 25.09"
                     /tool="antismash"
     misc_feature    complement(4950..4965)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 17.85"
                     /tool="antismash"
     gene            5001..5405
                     /locus_tag="PWG71_RS23750"
                     /old_locus_tag="PWG71_23750"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014910344.1"
                     /locus_tag="PWG71_RS23750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_23750"
                     /product="ectoine synthase"
                     /protein_id="WP_274812188.1"
                     /sec_met_domain="ectoine_synt (E-value: 4.5e-56, bitscore:
                     180.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLDEITDTEADIKTENWRSRRIVLAKEKVGFSFHETVLYAG
                     TESTFWYANHVELVHCVEGEAELTNEETGEKHIITPGTLYLLNGHERHTVRPKTDFRVL
                     CVFNPPVTGREVHDENGVYPLVVEEDGAPA"
     misc_feature    complement(5582..5595)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.96"
                     /tool="antismash"
     gene            5876..6790
                     /gene="ehuB"
                     /locus_tag="PWG71_RS23755"
                     /old_locus_tag="PWG71_23755"
     CDS             5876..6790
                     /GO_component="GO:0009897 - external side of plasma
                     membrane [Evidence IEA]"
                     /GO_component="GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033294 - ectoine binding [Evidence IEA]"
                     /GO_function="GO:0033295 - hydroxyectoine binding [Evidence
                     IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017603966.1"
                     /locus_tag="PWG71_RS23755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_23755"
                     /product="ectoine/hydroxyectoine ABC transporter
                     substrate-binding protein EhuB"
                     /protein_id="WP_274812189.1"
                     /transl_table=11
                     /translation="MQVPANRRSRFGRRDAFRLAGAGAVTLGLAACTRVDQGGNGEEGG
                     ASTLERLWEQGFVSVGLSNEIPFGFVDGSGEPAGQSPAVAQAVFEELGVPEIQASPVSW
                     DGLIPGLQANRFDVIAAGMFITPDRCEQVAFTEPTATSPEAFLVAEGNPHDIQHFTDFA
                     DNPDITLAVLNASVEQTYAEHFGVPTDQMRLIPDQTTGYELLDSGRVDAVSMLKVSHEY
                     LLLERGGDFEVTEAFFPEIDGREEKGWGALCVRPADSALLEEMNRVIAEFTESGRLLEL
                     GEEWGFTEADLPDGTTTDELCGD"
     gene            6798..7523
                     /gene="ehuC"
                     /locus_tag="PWG71_RS23760"
                     /old_locus_tag="PWG71_23760"
     CDS             6798..7523
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuC"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546871.1"
                     /locus_tag="PWG71_RS23760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_23760"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="WP_274812190.1"
                     /transl_table=11
                     /translation="MEFLIDRLPLYLGGAWVTVQLTVGGIALAFVLAMFFGVLGTARSG
                     LLRAVSTVYVEVFRGIAALVLMFWMAYALPLLTGFQLDGRFAAILAVGLNVGAYGAEVV
                     RGAINAVPRAQTEATIALDMSWAQRMRLVILPQAWAQMLPTFGNLSIELMKATAVAFLI
                     GVADITAVAEQMRQATGETVLAFSGALILYFLIAQVLIFGVRLLERRANRRLGRTPPGG
                     RGLLALLRPPALTTSGGAK"
     misc_feature    6819..6821
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            7524..8162
                     /gene="ehuD"
                     /locus_tag="PWG71_RS23765"
                     /old_locus_tag="PWG71_23765"
     CDS             7524..8162
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuD"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156204.1"
                     /locus_tag="PWG71_RS23765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_23765"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="WP_274812192.1"
                     /transl_table=11
                     /translation="MIWDFEWTWQVMPDLMQGLAVTVQATVFGYLIALVLGLAIAMVRR
                     VPVIGTIVHAVMEFIRTTPLLVQLVFVFALAPSTVSALTVGIVVLGVHYSAYTAEVYRS
                     GIEGVPKGQWEAARALSLPAGRTWGAVVLPQAIRKVIPALGNYLIAMFKDTPLLLAITV
                     TELLATARQVGSAGFRIVEAYTVVGFLFLLVSIPSAILIRRLERRYAAV"
     gene            8149..8940
                     /gene="ehuA"
                     /locus_tag="PWG71_RS23770"
                     /old_locus_tag="PWG71_23770"
     CDS             8149..8940
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657168.1"
                     /locus_tag="PWG71_RS23770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_23770"
                     /product="ectoine/hydroxyectoine ABC transporter
                     ATP-binding protein EhuA"
                     /protein_id="WP_274812193.1"
                     /transl_table=11
                     /translation="MPPSDAQALITFDQVVKRFGDLTVLDHLDFSVAPGERVTLIGPSG
                     SGKTTILRLLMTLERVTEGTIMVEGEPFSHMPRGDRLVPANEAYLSARRKRIGMVFQQF
                     NLFPNMTVRKNLTEAPVHVLKLSRAEADARAEELLELVGLSDKIDAHPTQLSGGQQQRV
                     AIARALAMRPEILLLDEVTSALDPELVAGVLDVLRTIAETTDITMLCVTHEMGFARDVS
                     HRVLMFDEGRIAEEGTPDEIFGDPREERTKSFLKSVLDNGH"
     gene            9091..10023
                     /locus_tag="PWG71_RS23775"
                     /old_locus_tag="PWG71_23775"
     CDS             9091..10023
                     /EC_number="3.4.-.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156206.1"
                     /locus_tag="PWG71_RS23775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_23775"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_274812194.1"
                     /sec_met_domain="Abi (E-value: 7.9e-15, bitscore: 47.8,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVRRWGLTGWTGAALLVVAVALLIDPALRNDIAVEGAFLLLVSWA
                     PMLIGGILAWALVKAFGDRDALDRRTAAAMESHPINRELVWLALFAVGFVVCMVVLSTI
                     LNMYGTQTGTDLAMSASLVSRLLFLFTLPLLVMDRSGVVIDGKGTAMPAIALKVDTPWR
                     WLGMIPVAVVLGLIGFTLVPYVGLPVLSFPLIGFVLAFAVISVCEEIFFRGMLQTRLEM
                     LMGRWGGIVATSVLFALVYAIVQPYDAVAQLPGNGLVYDMGLSLLTYATAGLLYGYLWT
                     CFRNTWLNVILRIGVFLVLMPPDLQTGIV"
ORIGIN
        1 acgccgccga ccccagcgcc tggctgatgt gcagcgacgg cctctccgac ctggtgctgc
       61 ccgaggagat ggaacgcatg atcgccgagg ccggcagtga cgaggccgcc gtccacgccc
      121 tgtggcaggc cgccatggag gccggcggca aggacaacat cagcatcctg ctcgcccgcc
      181 gcgtctgacg accgcgacac ccacccgatc caggggatac cggcccgttc cgtacgaggg
      241 gcccgcgcac cggaaggcgc gcgggcccct cgtcctctcg gccggtcccc gtgactactc
      301 ggtgggggag cccttgcgga gccggacggc cacgatcgcg atggcggcca gggcgatcac
      361 gcccaccacc acgagcacga tgacccccac gggcagcccg ccctcctcct cggcggcggc
      421 ctcggaggcc tcgacctcct ccgcgggggc gctctcggcg gccgtctcct cggcgacccc
      481 gcccggctcc gccgactcct cgggagcggc ggcggccacg gcctcctccg tcaccgtgaa
      541 cgtcaggctg tcctggatcg gatgtccgtc ggaggacacc acccggaagc cgatcgtgta
      601 ctcgccggcc tcctccaacg gcagcaggcc caccgagacg tccagcccgt cgaaggcggg
      661 ctcgccgtcc tcgtaggtgg tctccccgtc cggcccggtg acgacgacgg cgctgccgga
      721 gccgcccgtc atgggctcgt tgttgaagga gagcacgacc tcctccggga cggtgtccag
      781 ggtggcgccg tccttcgggt tcgaaccggt caggacgtcg tgcgccaggg ccggggcggg
      841 cgcagccatc agggcggcgg ccaccagcac ggccgggacg agggcgcgcc gcagggggga
      901 ggcgtgggta cgggtcattc ttctcttctt ctctcgggct cggtccgcac cgggaacgcc
      961 gtcggccgat cccggtctca tcgcgtgcgc cgctcaggcg gcgagggcga tggggggcgg
     1021 gccgcgcggt cggtgcgccg agagggccgg tgccgtcggg accgcagggg cgacggtcgc
     1081 ggcgatcccc ggggtcggcg ggagggggag ggcgcgggcg cgcgcgggag gcagcgcccg
     1141 ggacagcagg gcggccaggg accacagcgc cgactccccg tacgccaaca gcgccgaggc
     1201 cagcagggcg gcccacaggt gggccaagag catgccgggg gcgaacccga ccgcgtgcgc
     1261 gagcaccccg tggccggagt ggtcgacacc ggtgtcgaag gtcgcgaagg cggggtcggc
     1321 cgacccctgg aggatcaggt gcaggatcac ctggagggtg gccatgacgg cgaagatgtc
     1381 gccgaaaccg cgcatgacgc gggtgaagtg ccacagcacg ggcgcgagca cgaccgtggc
     1441 caggacgaat cctcccgggc cggccggtcc gccggcccac ggatcgtgtc cgacccaggc
     1501 cagacccgtg cacgcggcgg ccagtgacgc cgtgcgcagg aggcggagtg tgccgtgagc
     1561 gggtcgcacg tgagaaaacc tagatcctgt ccggtgggcc cgcaagggcg cgaagaggcc
     1621 gtcgggtgcg atcccccgcg cacccgacgg ccacatcggt ttccgggccg accgttccct
     1681 tccacgcggg cccgcccgtc cggggacggg tcctcttccc ggtcctacac cgcgggagcc
     1741 agctcgcgac gcatgtagcg acgctcctcc ggggtggtgc caccccagac tccgtgggcc
     1801 tccccggacc gcaacgccca ccgcaggcac tccgggcgaa cagtacagcc gcggcagacg
     1861 gcgagggcct gttcggtgtc ggccctgccg atcccggtgc tgctcaccgg gaagaagatc
     1921 tcggggtcgt acgcacggca actcccctgc gtgacccagt cctcgctctc gtggtacagg
     1981 cggttcacgt ggtgcctccg atccgtggct gtgtgtccct ggccggtggc ccgcaccgtg
     2041 tcccccacgg ccgggtcggc ggaagtcgtc gcgccccgcc gcggcccgag ccctcggtgg
     2101 agggccggtg gggtgaccgt agggttgtcc ggtcgcccgg aactgcggcg aagcgatgcg
     2161 actaagtgta gtactacatc gggtcgtact acacggggtc tgcttggaaa cccgcccgat
     2221 tcccagggac tttcgtcggt tttcgagtcc cgtgcgcccc atcgggggtc ggggacgcgg
     2281 cgcgcgcggg ccgcgccggg ccgggggtcg gggacggccg gagcccttgc gtggagcggc
     2341 tttcgtcgag gccgggaggg gttcgagggc attcgcgcgg cgggggcgcg gcacctcggc
     2401 gccacggggc cgccgcgagc atggcgttat gtcgcgaggt ttccaccgaa ttgatgtgac
     2461 ttaacgcacg cttaatattg ctcgttcagg ctctatgttt gaccccgaca cccgtggccc
     2521 gcattctcgc gccgagggtg ccgccgcgtc atcacgacac gcggccccac ctcgggcccg
     2581 acgccgaatc ccctgatggg cagggcgtgc gccactccat cgatcgtcgc gcggcggggt
     2641 gcgagagccc tggtcacggg cttggaacgg gactaaagaa acaagcccta cccgatcacc
     2701 ttcccgcgaa acatccgcga ggattccctc ggtaatccca ttgaccttcg ttcgctattt
     2761 tcgttcgacg tgccgcatct acgaacgaat ctccaagccc gcgaccgtat cgacaacgga
     2821 gcgcgcggct ccgcacatcg tgccggtgcc gcgagccctg gggacgtgcg cctggcacat
     2881 cccgaacccg aggacggccc gcacctgtgg tccctggccc ggaccacggg tatggacctg
     2941 aactctccgt acgcttacac actctggtgc cgtgacttcg cggcgacgag cgtcgtcgcc
     3001 cgtgacgaca ccggccgacc gatcgcttac ctcaccggtt acgtgcgacc cgacagcccg
     3061 gacacctact tcctctggca ggtcgccgtc gacccggccc atcggggccg ccgcctggcc
     3121 cggtacatgc tcgacttcat cggtgatcgg atcgtcgaac gagggatcga gcacctggag
     3181 gcgaccgtca ccccggacaa caccgcgtcg cgtgcgctgt tcgcatcgtt cgcacgcgat
     3241 cgcggggcac ggatcacctg gaccgcactc ttctccgagg agcactttcc gaaggaggac
     3301 ggggaatccc acgagcccga agacctcgtg cgcatcggcc ccctggggct ccccgacgaa
     3361 gagcaggcgt gagtgccacc ggccacccgc accggatcga tcgcttcggc gatcggacat
     3421 accctgagcc cgagaacctg gggaaaggaa ccgaggaacc cacgatggag accttcgagc
     3481 gcctggaatc cgaagtccgc ggatactgcc ggaactggcc ggtggtcttc gaccgtgccg
     3541 tcggaagcca tgtctatgac gagacgggcc gtccctacct ggacttcttc gcgggagccg
     3601 gatccctcaa ctacgggcac aacaacccgg agctgaagtc cgcgctcatc cgctacctca
     3661 ccgacgaccg catcgtccac agcctggacg cctacagcgt cgccaaacgc gacttcctgt
     3721 cgaccttcga ggagatcgtc ctcaaaccgc gagggctgga ctacaaggtc cagttccccg
     3781 gcccggcggg caacaacgcc gtcgaggccg cactcaagct ggcccgcaag tacaccggcc
     3841 gcgagacgat cgtcaacttc accaacggct tccacggcat gaccctgggc gcgctggccg
     3901 tcaccggcaa ctcgatgaag cgcgggggcg cgggcgtgcc cctgggccac gtcgccacca
     3961 tgccgttcga caactacatg gacgggcgca ccccggactt cctctggctg cgcagcctcc
     4021 tggaggacag cggcagcgga ctggacaagc cggcggccgt catcgtcgag tcgatccagg
     4081 gcgagggcgg catcaacgtc gccggcgccc agtggctgcg cgagctgtcc gagctgtgcc
     4141 gtgagtacgg catcctcatg atcgtcgacg acatccagat gggctgcggc cgtaccggcg
     4201 acttcttcag cttcgaagag gccggtatcg ttccggacat cgtcaccctg tcgaagtcga
     4261 tcagcggcta cggtcttccc atggcactga ccctgttcaa gcgcgaactg gacgtgtggg
     4321 agcccgggga gcacaacggg accttccgcg gcttcaaccc cgccatggtc accgccaccg
     4381 aggcgctgcg ccgttactgg acggacgacg ccttcgccaa cgcgaccaag gccaagggcg
     4441 acctcgtggc cgcccgcctg gacgagatgg ccgccgagca cgccgagttc ggcgcccacg
     4501 tgcgcggtcg gggcctggcc cgcgggctgg ccttcaccga caccgacctg gccaagaagg
     4561 tcgccgccga gtccttcgac cgagggttgc tcctggagac ctccggtccc gaggacgagg
     4621 tggccaaact cctgccgccg ctcaccatga cggacgagga gctcacggcc ggcctcgaca
     4681 tcatggccga cgcggcgcgc gcggcggcca aggtggccca acccgcgtaa gtccgccgtt
     4741 cgtcaccttc ggtaccgcag gaggtcgcga ggcgccggcg gggttctgga ggcccggagc
     4801 gaacgtccga ggagcgagga tgttcgcgta gtggccgagg gtccccgcct tccgaggcgc
     4861 cgagtacgaa ccgaagcgga gcgggggtcc gaagcggcat cgcggggtcg gtcggtcacc
     4921 cgttcggggc ggccgaccgg ccccgcgtcg ttcacaccgg tagtcacgtg cgcacgagcg
     4981 caccaacgag gaggaaccct gtgatcgttc gcagcctgga cgagatcacc gacaccgagg
     5041 ccgacatcaa gacggagaac tggcgcagcc gccggatcgt cctggccaag gagaaggtgg
     5101 gcttctcctt ccacgagacc gtcctgtacg cgggcaccga gtcgacgttc tggtacgcca
     5161 accacgtcga gctggtgcac tgcgtcgagg gcgaggcgga actgaccaac gaggagacgg
     5221 gggagaagca catcatcacc ccgggcacgc tctacctcct caacggccac gagcggcaca
     5281 ccgtccggcc caagaccgac ttccgcgtcc tgtgcgtgtt caacccgccg gtcaccggtc
     5341 gagaggtcca cgacgagaac ggcgtctacc cgctggtcgt cgaagaggac ggcgcccccg
     5401 cctaggcacc cgcacgccgg ggcgtccacc ccgtccggcg gatcaccacc ggagaagacg
     5461 tctccgcgaa ggggcaccgg cgcgcgctcc gcgcccgacg gtgccccttc gcgtgggccg
     5521 ggaaccggcg ggcatcgggc ctccgggcga acccggacgg gcccgccctc gcctcaatcg
     5581 cgcactcaga gtgatgaact ctgcactcag gggtatatat cggcgtgaga acggatgctt
     5641 ttccgtcctc ggataaccag tacataacgg acccttgggg tctcgttacc tccgtggggc
     5701 gtcggggagg gagaaccccg tacccggggc ccgagtacgt cgtcgaccga cgtcggcgcg
     5761 ggtcggggcc gctccgagcg gtctccggcc gccgggtctc cttctgcgcg gcgccccccg
     5821 gggtcacccg ggggcgcgaa ggatcgcgcg agtgatgcga ggagtgagag cgaagatgca
     5881 ggtaccagcg aacaggcgga gtcgtttcgg tcgcagggac gcgttccgtc tggccggtgc
     5941 gggggccgtc accctcgggc tcgccgcgtg tacgcgcgtc gatcagggcg gcaacggcga
     6001 agagggaggc gcctccacac tggagcgact ctgggaacag ggtttcgtgt ccgtcgggtt
     6061 gtccaacgag atcccgttcg ggttcgtcga cggttcgggt gaacccgccg ggcagtcccc
     6121 ggccgtggcc caggccgtct tcgaggaact gggcgtcccc gagatccagg cgtccccggt
     6181 ctcctgggac gggctcattc cggggcttca ggccaaccgg ttcgacgtca tcgccgcggg
     6241 catgttcatc actccggacc gttgcgaaca ggtggccttc accgagccca ccgcgacctc
     6301 gcccgaggcg ttcctggtcg ccgagggcaa tccgcacgac attcagcact tcacggactt
     6361 cgccgataac ccggacatca ccctggcggt gctcaacgcc tcggtggagc agacctacgc
     6421 cgagcacttc ggagtcccca cggaccagat gcggctgatc cccgaccaga ccaccggcta
     6481 cgagctgctg gactccggcc gggtggacgc cgtctccatg ctcaaggtct cccacgagta
     6541 cctgcttctg gagcgcggcg gcgacttcga ggtcaccgag gcgttcttcc ccgagatcga
     6601 cggtcgtgag gagaagggct ggggcgctct gtgcgtgcgc cccgcggaca gcgcgctcct
     6661 ggaggagatg aaccgggtca tcgccgagtt caccgagagc ggtcgcctgc tggaactggg
     6721 cgaggagtgg ggcttcaccg aggccgacct acccgacggc accaccaccg acgagctctg
     6781 cggggactga tcccgccgtg gagttcctca tcgaccgctt accgctctat ctgggcggcg
     6841 cgtgggtcac cgtccagctg accgtcggcg gtatcgcgct ggcgttcgtc ctggccatgt
     6901 tcttcggcgt cctggggacc gcgcgcagcg ggctgctccg tgccgtgtcc acggtctacg
     6961 tcgaggtctt ccgcgggatc gccgcgctgg tcctgatgtt ctggatggcc tacgcgctgc
     7021 ccctcctgac ggggttccag ctggacggcc gattcgccgc gatcctcgcc gtgggcctca
     7081 acgtcggggc ctacggcgcc gaggtggtgc gcggtgcgat caacgccgtg ccccgtgccc
     7141 agaccgaggc gaccatcgcg ctcgacatgt cctgggccca gcggatgcgc ctggtgatcc
     7201 tgccgcaggc ctgggcgcag atgctgccga ccttcggcaa cctgtccatc gagctgatga
     7261 aggccaccgc cgtcgccttc ctgatcggcg tcgccgacat caccgccgtc gccgaacaga
     7321 tgcgccaggc caccggcgag acggtactgg ccttcagcgg tgccctgatc ctgtacttcc
     7381 tcatcgccca ggtgctcatc ttcggcgtgc gcctcctgga acggcgtgcc aaccgcagac
     7441 tcggtcgcac acctccgggc ggccgggggc tcctcgccct gctgcggccg ccggcgctca
     7501 ccacatcggg gggtgcgaag tagatgatct gggatttcga atggacctgg caggtgatgc
     7561 cggacctcat gcaggggctg gcggtcaccg tccaggcgac cgtgttcggg tacctcatcg
     7621 ccctggtgct gggcctggcc atcgcgatgg tccggcgtgt tccggtgatc ggcacgatcg
     7681 tgcacgcggt catggagttc atccgcacca ccccgttgct ggtccagctg gtgttcgtgt
     7741 tcgcgttggc gccttccacc gtgtcggcgc tgaccgtcgg catcgtcgtg ctgggcgtgc
     7801 actactcggc ctacaccgcc gaggtgtacc ggtccggcat cgagggggtg cccaagggcc
     7861 agtgggaggc cgcccgggcg ctgagcctgc ccgctgggcg cacctggggc gccgtggtgc
     7921 tgccccaggc gatccgcaag gtcatccccg cgctgggcaa ctacctcatc gccatgttca
     7981 aggacacccc gctgctgctg gcgatcaccg tcaccgaact gctcgccacg gcccgccagg
     8041 tcggcagcgc cggcttccgc atcgtggagg cctacaccgt ggtcggattc ctcttcctcc
     8101 tggtgagcat cccctccgcg atcctcatcc gacgactgga gcgacgctat gccgccgtct
     8161 gacgcccagg cgctgatcac cttcgatcag gtggtcaagc gcttcggtga cctcaccgtg
     8221 ctggaccacc tggacttctc cgtggccccc ggcgaacggg tcaccctgat cgggccgagc
     8281 ggatcgggca agaccaccat cctgcgcctg ctcatgaccc tggagagggt caccgagggc
     8341 acgatcatgg tggaggggga accgttcagc cacatgccgc gcggcgaccg cctggtcccg
     8401 gcgaacgagg cctacctgag cgctcgccgc aagcgcatcg gcatggtgtt ccagcagttc
     8461 aacctgttcc ccaacatgac ggtccgcaag aacctcaccg aggcgccggt gcacgtcctg
     8521 aagctctccc gggccgaggc cgacgcgcgc gccgaggagc tgctggagct ggtgggactg
     8581 tcggacaaga tcgacgccca tcccacgcag ctctcgggcg gccaacagca gcgggtggcg
     8641 atcgcccgcg cgctggcgat gcggccggag atcctgctgc tggacgaggt gacctcggcg
     8701 ctggaccccg agctggtcgc cggggtgctg gacgtactgc gcacgatcgc cgagaccacg
     8761 gacatcacga tgctgtgcgt gacccacgag atggggttcg cgcgggacgt gtcgcaccgg
     8821 gtgctcatgt tcgacgaggg gcgcatcgcc gaggagggga cgccggacga gatcttcggc
     8881 gacccgcggg aggagcgcac caagtccttc ctcaagtccg tgctggacaa cggacactga
     8941 cgtacgcgcg gccccgggtg ggcgatgccg gctccggcgt cgcccaccct ccgacttcga
     9001 cctggtgacg acataccgta gtcgttaagt agcttttagt ttccccttgg gcagaatgag
     9061 tgctctgaga caggggggac tcttgtgacc atggtgcgaa gatgggggct caccgggtgg
     9121 acgggcgccg ccctcctggt ggtggcggtg gccctgctca tcgaccccgc cctgcgcaac
     9181 gacatcgccg tcgagggcgc gttcctgctc ctggtctcct gggcgcccat gctcatcggc
     9241 ggcatcctgg cctgggcgct ggtcaaggcc ttcggggacc gggacgcact ggaccggcgc
     9301 accgccgccg cgatggagag tcatcccatc aatcgcgagc tggtgtggct ggccctgttc
     9361 gcggtgggat tcgtggtgtg catggtcgtg ctgagcacca tcctgaacat gtacggaaca
     9421 cagaccggaa ccgatctggc catgtcggcc tccctggtgt cccggctgct gttcctgttc
     9481 accctgccgc tcctggtcat ggaccggtcc ggtgtggtca tcgacggcaa gggcaccgcc
     9541 atgcccgcca tcgcgctcaa ggtcgacact ccctggcgct ggctgggaat gatcccggtg
     9601 gccgtggtgc tggggctcat cggcttcacg ctcgtcccct acgtgggcct gccggtcctg
     9661 tccttcccgc tcatcgggtt cgtgctggcg ttcgcggtga tctcggtgtg cgaggagatc
     9721 ttcttccgcg gcatgctaca gacgcgcctg gagatgctca tggggcgctg gggagggatc
     9781 gtggccacgt cggtgctgtt cgcgctcgtc tacgcgatcg tccagcccta tgacgcggtg
     9841 gcccagctcc ccgggaacgg tctggtctac gacatgggac tgtccctgtt gacctatgcg
     9901 accgcgggtc tgctctacgg ctacctgtgg acgtgcttcc gcaacacctg gctcaacgtg
     9961 atcctgcgga tcggggtgtt cctcgtgctg atgcccccgg acttgcagac cgggatcgtc
    10021 taggacgatc accaacaggt ggcactgaag gtccgactct cccctcgacg tcccctcgcc
    10081 cccgccccgc cggcgccccc attgccgcct ccacgagccg aaacctgcgc aaactatgct
    10141 cgataagtgc aggtgttcgt gtgcatctgg gtacagaagc tccatagcgg tcgtgttgcg
    10201 gtcattttcc cctcatgggg ttgtcaccgg ccggtcatga aaagggggga gtctgccagc
    10261 tgtgagcacg gaacccgtat cgacgtcagc cggtcccagt accgccgaac ctcccgaact
    10321 gccctccgac aggttcatgg acagggagga gggctggctc cgcttcaacc agcgtgtcct
    10381 cgaactggcc gaggacgacg acatc
//
