LOCUS       NZ_JARBUT010000016     11044 bp    DNA     linear   CON 29-APR-2024
DEFINITION  Nocardiopsis sp. N85 Scaffold16, whole genome shotgun sequence.
ACCESSION   NZ_JARBUT010000016
VERSION     NZ_JARBUT010000016.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nocardiopsis sp. N85
  ORGANISM  Nocardiopsis sp. N85
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsaceae; Nocardiopsis.
REFERENCE   1  (bases 1 to 153433)
  AUTHORS   Liu,S.
  TITLE     Nocardiopsis sp. N85
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 153433)
  AUTHORS   Liu,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Microbial Chemistry, Institute of Medicinal
            Biotechnology, Chinese Academy of Medical Sciences, Tiantanxili No.
            1, Beijing 100050, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 1.05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 182.0x
            Sequencing Technology :: combinatorial probe-anchor synthesis
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028882275.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 06:01:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,663
            Genes (coding)                    :: 5,611
            CDSs (with protein)               :: 5,611
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 46 of 53
            Pseudo Genes (internal stop)      :: 0 of 53
            Pseudo Genes (multiple problems)  :: 3 of 53
            CRISPR Arrays                     :: 13
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:47:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 66021
            Orig. end                         :: 77065
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JARBUT010000016.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11044
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="2"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11044
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11044
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6044](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6044)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(236..2554)
                     /locus_tag="PWG71_RS20805"
                     /old_locus_tag="PWG71_20805"
     CDS             complement(236..2554)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1035: MMPL domain-containing transport protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /locus_tag="PWG71_RS20805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_20805"
                     /product="efflux RND transporter permease subunit"
                     /protein_id="WP_274811598.1"
                     /transl_table=11
                     /translation="MLDALTGLTTRRPWWVLAIAFLAVIGAVILGSGVADRLRAGQGTE
                     DPSSESALAAEILEDHFPDGRPNLILLVTAEEGVDDREVAARGLGLAEGLAYEDAVAGV
                     TSYWQTGSDELKSDDGTRALIAAHVSGTEAEAGEAVADLAERYGGDHGPLTVGVGGQTA
                     VLNDIEATIAEDLIRSEMIALPLTLLILVWVYGSVVSATLPLLVGVVSIIGTNAVLWAI
                     AGFTDVSVFAQNLTIALGLGLSIDYALLMVRRFRAELASGAEPSAATLTTVRTAGRTVV
                     FSALAISAALIAMLFFPLYFLRSFAYSGVTVVLLAALTSLIVLPAVLTLLGPRVNALDP
                     RRLLRRGSALSPEEAADRRWGRLTRTVMGRAPVFALVALTLLIVAGLPFLRVEFGMADD
                     RQLPAGVESREVMDTIRTDFGSTATGAVDVLVHEAPEDTDTELLDDYVGALSRLDHVEE
                     VRSPLGVHIDGAHLRERTPVDALRESEDLAYIQVLPDAEVEDISPESQNLVRDIRAVDT
                     PLEVSVAGQAAAVVDAQEAIAERIPLALTVVVAGMLVLVYLLTGSVLLPVLSVLFNALS
                     LTAMFGAVVWVFQDGNLSGPLGFTPTGFIDTALPVLMFCIAFGLSMDYGVFLLARMREE
                     YDRTGDHRAAVELGLRRTGGVITAAAVALAVVLIVIGASRVTNTMMLGWGVALAVIADA
                     TVVRCLLVPSVLRMTGRATWWAPGPLRRFHRAFDATLGENAADPARVPAGPSPVVPPPV
                     AERDAEPVRENAEVARARD"
     misc_feature    2648..2661
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.4"
                     /tool="antismash"
     gene            complement(2699..4336)
                     /locus_tag="PWG71_RS20810"
                     /old_locus_tag="PWG71_20810"
     CDS             complement(2699..4336)
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017564325.1"
                     /locus_tag="PWG71_RS20810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_20810"
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_274811599.1"
                     /transl_table=11
                     /translation="MSAPGPHPPVLVVGAGPTGLAAACTLLQNGVPCRVVERRAGVAEE
                     PKALILWSGALEVLRRLGVSEEVVDRSLPLESASYFSRGRRIGGVRFGGLSDTAFPGPI
                     CLPQPGTEQALYDRLLALGGSVEWSTRVHTAEPTEDGEGVRVVLGPDGDAHTREEVVVP
                     WVVAADGSRSAVREALGVPFEGDTYDRVFLLADGVVSGPTPVREAQYHLTPDGVLVVVP
                     LPGGGHRVFFDIAPDGETAPPDDALIARLLAERGPRGLRVDTVWWRSRFRVHARVAREF
                     RRGRVLIAGDAAHLHSPAGGQGLNTGIQDGFDLAWKLASVLRGAPETLLDSYPLERRPI
                     ARTVVRSADQQTRTWMARSPLARLVRDTLMRVLSASGLLEKRLVPQLAQISPDLTESPA
                     VVAPPAGWRARDAVGGALPGRRIGDAPLSPLQGTDATTLHAYLASGRHALLVGGGSSRA
                     REAAAELRGRLPDDVDVLLLAPSAVEAAGDHDVALLPGRGGEGAGAEWVIHVRPDAVVA
                     SVAPLDARWRPGVFLGATLLPDAERSLK"
     gene            complement(4333..5004)
                     /locus_tag="PWG71_RS20815"
                     /old_locus_tag="PWG71_20815"
     CDS             complement(4333..5004)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017564324.1"
                     /locus_tag="PWG71_RS20815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_20815"
                     /product="HAD family phosphatase"
                     /protein_id="WP_274811600.1"
                     /sec_met_domain="HAD_2 (E-value: 1.5e-14, bitscore: 47.5,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRIEAVVCDYGGVLTNPLHETLEHFAGTAGLSADAILTAIGAAA
                     AKGGADPMALLEVGAITEADLVAQITAELPEGGWTLPEDRTFGELWFAGRTGNHGFADF
                     LRDLNMEGYRLALLTNNVLEWEELWRATVPADELFEVVVNSAHEGVRKPDTEIYERLLA
                     RLGLTPDRCLFVDDLEANLKPAAELGMATVRFQDNAQAIAEITELLDGAGRPARAAEAP
                     R"
     gene            complement(5001..6044)
                     /locus_tag="PWG71_RS20820"
                     /old_locus_tag="PWG71_20820"
     CDS             complement(5001..6044)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="PWG71_RS20820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_20820"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_274811601.1"
                     /sec_met_domain="AfsA (E-value: 5.6e-19, bitscore: 61.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNRTTADAPSAVAEVTDSAQRALADLDPENTGIRELDYDATVPRG
                     LVHRKAVSEVFVTDSRQTGENTFDVAAQLPRGHVMLETPTYDLPLILEACRQTGVLISH
                     RHLDVPMDRAFIMHRMSLHIGDLDGLRQGAEPARLMVATEADLYRNQAGRLRGYDFRGT
                     VLLNGTEVAQATGALIFLSRQAYQALRTKGRESLDWSAAAAPRAIPAQPSVVGRRDPRN
                     VVITDPVQRGDESWRATVVPDHSHPHLFDHPLDHLPGNLLIEAARQVAVAGVARSEGLD
                     PVTLVPVSVEASFSSFCENDLPSTLEADITPFRSDERFGPVVGVDVRVNQQGTTNSSFR
                     IEVAQWV"
     misc_feature    6223..6236
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.34"
                     /tool="antismash"
     misc_feature    complement(6223..6237)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.21"
                     /tool="antismash"
     misc_feature    6224..6237
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.59"
                     /tool="antismash"
     misc_feature    6224..6238
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.29"
                     /tool="antismash"
     misc_feature    6238..6247
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.04"
                     /tool="antismash"
     misc_feature    complement(6261..6276)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 18.27"
                     /tool="antismash"
     misc_feature    complement(6316..6331)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.52"
                     /tool="antismash"
     misc_feature    6494..6511
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 19.9"
                     /tool="antismash"
     gene            6609..7415
                     /locus_tag="PWG71_RS20825"
                     /old_locus_tag="PWG71_20825"
     CDS             6609..7415
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /locus_tag="PWG71_RS20825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_20825"
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_274811602.1"
                     /transl_table=11
                     /translation="MIRTRRTAPTTVSVLGELEVIGDHGPLPLGAPRQRAVLAALVLNG
                     DRPLTTQRVITMVWGSTPPAYAVNLVHKYMSGLRRALIDLPPHKRVDIESGPSGYRLVA
                     PVGQVDLALFQTWIDAARDLRARGDGERAAELFGRALDLWRGPLLGELSGSAFDEEREF
                     METVRLAVLEEYATVAIEVGRQADVVESLREALRRHPLEENLARLLILALYSEGRTAEA
                     VRVYQRLTSHMLGELNVPPRPELHDLYQIIRRHGPIPTGRRLQHIP"
     gene            complement(7512..9722)
                     /locus_tag="PWG71_RS20830"
                     /old_locus_tag="PWG71_20830"
     CDS             complement(7512..9722)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1035: MMPL domain-containing transport protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019608306.1"
                     /locus_tag="PWG71_RS20830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_20830"
                     /product="MMPL family transporter"
                     /protein_id="WP_274811603.1"
                     /transl_table=11
                     /translation="MPTHTGLFGRLGLGAHRFRWAVLVTTAVFAVFAVVWGSGVFSDVS
                     ESGFEDPGAESSRAMDVLEGELGHDDVDIVAVYRSDEMKVDNPTFAAAIQRIADDMPRG
                     VVADFDVYLDEGLSDIERGMLVSEDMHATYIPITLTGESHPERLDQYDDVAEHLASGPL
                     ETHLGGPIAVEHELSEAAESDVVRAELITLPLLLFLLVLIFGGLIAGVVPLAVGGLAIL
                     GSLVTLRVLTHVTEVSVFAVNVATILGLGLAIDYGLFMVSRFREELARGRGVAAAVGRT
                     VDTAGRTVAFSGVTVGIAFAGLLFFPQPILKSIGFGGIAVVLFDLLAALVFLPALLSVV
                     GHRIDGLRLPLPRRTVTGTLHEGGWTRLARTVMRGPAVSLVAVLGVLVIFGSGLVSAHL
                     GTTDQRYLPQDAGSRIATESVAEDFPGGGLGRLHVAVVGDAGDEALDDYVERLEGLPEV
                     TLARVERTGDGIAHVLVAYQGAADSDEIKGLVADVRAEPAPDGAEEALVGGVAAMQQDN
                     VAAIVAAAPTTIGFIAGATLFLLFLAFGSVVLPVKAVLMGALSLSASLGVVVWGFQEGA
                     FAGLLGFEAVGTIDPTYLVLITVVAFGLAMDYELFLLSRVREEYLRTGDNTRSVATGLQ
                     HTGRIITSAAILLMVVLLAMGASDLLFLKIIGIGLALAVLVDATLVRAIMVPATMRLLG
                     GVNWWLPRPLRWLHDRIGISEGGDEEPSVRVSDERERVPAGV"
     misc_feature    complement(9764..9779)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: strong, score: 29.75"
                     /tool="antismash"
     misc_feature    9820..9833
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.89"
                     /tool="antismash"
     misc_feature    complement(9820..9836)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.33"
                     /tool="antismash"
     gene            9852..10634
                     /locus_tag="PWG71_RS20835"
                     /old_locus_tag="PWG71_20835"
     CDS             9852..10634
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013153863.1"
                     /locus_tag="PWG71_RS20835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_20835"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_274811604.1"
                     /transl_table=11
                     /translation="MTKPQLERVERVPSRRERVREATLSEIKAIARSHLVEHGVAGVSL
                     RAIAREMGMTAPGLYRYVSGIDSLLVLITADMFDELAAAVSDADASVPAEDTDRRINTS
                     LRAFRGWALEHPAEFAVMFGPRKRLEQEVDLTPAVEAGSRFGATFFTLFERLIDEARFE
                     LPDSGRVTPELARELRAFAVTCGFAGSETSVEAMMVLSSCWVRLYGVVCMEVFQHLDFV
                     MRDMEPLFESELQNVLTGLGVRYEAPQSNETATISTPD"
ORIGIN
        1 ggtgtactcc tcgccgtcgc tgttgcaggg caaccgcgag tcgttgcggt tgacgacggt
       61 gaagcggacc gggacgtcga cgatgtcggc ggcggccatg cgctcggcgg tggcccgcgc
      121 gcgttcctcg gccgtgggtt cgggtgcggc ggcgggtccg ttctcctcgg aggggagcag
      181 tatcagggcg gcggcggtca ggacgacggc cgcgcccgcg gcgatcaggg tgttcctagt
      241 cccgggcacg ggccacctcc gcgttctctc ggacgggttc ggcgtcgcgt tccgccaccg
      301 ggggcgggac caccggtgac ggcccggcgg ggacgcgggc ggggtcggcc gcgttctcac
      361 cgagggtcgc gtcgaaggcc cggtggaagc ggcgcagcgg gccgggcgcc caccaggtgg
      421 cacggccggt catgcgcagg accgagggca ccaggaggca gcgcacgacg gtggcgtcgg
      481 cgatgacggc cagggccacg ccccagccga gcatcatcgt gttggtcacc cgcgaggcgc
      541 cgatgacgat gaggacgacc gccagggcga cggcggcggc ggtgatgacg ccgccggtgc
      601 gccgcagtcc gagttcgacg gccgcgcggt gatcgcccgt gcggtcgtac tcctcgcgca
      661 tcctggccag gaggaagacc ccgtagtcca tggacagccc gaaggcgatg cagaacatga
      721 gcaccggcag ggcggtgtcg atgaacccgg tcggggtgaa gccgagcggc ccggagaggt
      781 tgccgtcctg gaagacccag acgacggcgc cgaacatcgc ggtgaggctg agcgcgttga
      841 acagcaccga cagcacgggc aggagcacac tgccggtgag caggtagacc aggacgagca
      901 tgccggcgac gacgacggtc agggcgagcg ggatgcgctc ggcgatggcc tcctgggcgt
      961 ccacgacggc ggcggcctgg ccggcgaccg agacctccag gggggtgtcg accgcgcgga
     1021 tgtcgcggac caggttctgg ctctcgggtg agatgtcctc gacctcggcg tccggcagga
     1081 cctggatgta ggccaggtcc tcggattcgc gcagggcgtc cacgggagtg cgttcacgca
     1141 ggtgggcgcc gtcgatgtgg acgcccaggg gggaacggac ctcctcgacg tggtccaggc
     1201 gcgagagggc gccgacgtag tcgtccagca gttcggtgtc ggtgtcctcc ggggcctcgt
     1261 ggacgagcac gtccacggca ccggtggcgg tggagccgaa gtcggtccga atggtgtcca
     1321 tgacctcccg ggactccacc ccggcgggca gttggcggtc gtcggccatg ccgaactcga
     1381 cccggaggaa gggcagtccg gccacgatca ggagggtgag ggccaccagg gcgaacacgg
     1441 gggcgcgccc catgacggtc cgggtgagcc ggccccagcg ccggtcggcg gcctcctccg
     1501 gggagagggc ggagcctcgg cgcagcaggc ggcgcgggtc cagcgcgttg acgcgcgggc
     1561 ccagcagggt gagcacggcg ggcaggacga tcagggaggt cagcgccgcc agcagcacca
     1621 ccgtcacacc ggagtaggcg aaggagcgca ggaagtagag ggggaagaac agcatcgcga
     1681 tcagggcggc cgagatggcc agggcggaga agacgacggt gcgtcccgcg gtgcgcacgg
     1741 tggtgagcgt ggcggcggac ggttcggccc cggacgcgag ttcggcccgg aacctgcgga
     1801 ccatgagcag ggcgtagtcg atcgacagac ccaggccgag cgcgatggtg aggttctgtg
     1861 cgaagaccga cacgtcggtg aatccggcga tcgcccacag caccgcgttg gtgccgatga
     1921 tggagaccac cccgaccagc aggggcaggg tcgccgagac gacgctgccg tagacccaca
     1981 ccaggatgag cagggtcagg ggcagggcga tcatctccga ccggatcagg tcctcggcga
     2041 tggtggcctc gatgtcgttg aggacggcgg tctggccgcc gacgccgacg gtgaggggcc
     2101 cgtggtcgcc gccgtagcgt tcggcgaggt cggccacggc ctccccggcc tcggcctcgg
     2161 tgccggagac gtgcgcggcg atgagcgcgc gcgtgccgtc gtcggacttc agctcgtcgg
     2221 aaccggtctg ccagtaggag gtgacgccgg cgacggcgtc ctcgtaggcc agtccttcgg
     2281 ccagtcccag tccgcgggcg gcgacctcgc ggtcgtccac gccctcctcg gccgtcacca
     2341 acaggatgag gttgggtctg ccgtcgggga agtggtcctc caggatctcc gccgccaggg
     2401 ccgattccga ggacgggtcc tcggtgccct ggcccgcccg gaggcggtcg gcgacaccgg
     2461 agcccaggat caccgcgccg atcaccgcca ggaacgcgat cgccaggacc caccagggcc
     2521 tgcgcgtggt gaggccggtc aacgcgtcga gcatcgtgtc acccccgttc gtcgtgcgcg
     2581 ggcggaaccg ccggtggcgg ccggagatct ccggccgcca ccacgggtgc cgtcgccgtc
     2641 acagtatcgt ataaacatgc gacgatcatc aacctaaacg atagggttca tatatcattt
     2701 acttgaggga ccgctcggcg tcgggcaaca aggtcgctcc gaggaacacc cccggtcgcc
     2761 aacgcgcgtc cagaggggcg accgaggcga cgaccgcgtc gggccggacg tggatcaccc
     2821 actcggcccc ggccccctcg ccccctctcc cgggcagcag ggcgacatcg tgatcgcccg
     2881 ccgcctccac cgccgacggg gcgagcagga gcacgtccac gtcgtcgggg aggcgccccc
     2941 gcagctcggc cgccgcctcc cgcgcccgcg acgagccgcc gcccaccaac agcgcgtgcc
     3001 gcccggaggc gaggtaggcg tgcagggtgg tcgcgtcggt cccctggagc ggggacagcg
     3061 gggcgtcgcc gatgcgccgt cccgggaggg ccccgcccac cgcgtcgcgg gcccgccagc
     3121 cggcgggcgg cgcgaccacg gccgggctct cggtcagatc cggggagatc tgcgccaact
     3181 gcggcaccag gcgcttctcc agcagccccg acgccgacag gacgcgcatc agggtgtcgc
     3241 gcaccaacct ggccagaggg gaacgggcca tccaggtacg ggtctgctgg tcggcgctgc
     3301 gcaccaccgt gcgggcgatc ggacgacgct ccagcggata gctgtccagc agcgtctccg
     3361 gagccccgcg caggaccgag gcgagcttcc aggccaggtc gaacccgtcc tggatgcccg
     3421 tgttgaggcc ctgaccgccg gccggactgt gcaggtgggc ggcgtctccg gcgatgagca
     3481 cccggccgcg ccggaactcg cgcgccaccc gggcgtggac ccggaaccgg ctgcgccacc
     3541 acacggtgtc cacgcgcagt ccacggggac cgcgctcggc cagcagccgg gcgatgagcg
     3601 cgtcgtcggg cggcgcggtc tccccgtccg gagcgatgtc gaagaagacc cggtggccgc
     3661 cgccgggcag cggcacgacc acgagcaccc cgtcgggggt caggtggtac tgggcctccc
     3721 ggaccggggt cgggccgctc acgacaccgt ccgccaggag gaacacgcgg tcgtaggtgt
     3781 cgccctcgaa gggcaccccc agtgcttcgc gcaccgcgct gcggctgccg tcggcggcca
     3841 ccacccaggg gacgacgacc tcttcccggg tatgggcatc gccgtcgggg ccgaggacca
     3901 cccgcacccc ctccccgtcc tcggtcggtt ccgccgtgtg gacccgggtg gaccactcga
     3961 cggaccctcc gagggcgagc aggcgatcgt agagggcctg ctccgttccg ggctggggca
     4021 ggcagatcgg ccccgggaac gcggtgtcgc tcagcccgcc gaagcggacc ccgccgatgc
     4081 gcctgcccct ggagaagtag gaggccgact ccaacggcag ggaccggtcg accacctctt
     4141 cggacacgcc caacctgcgc aggacctcca gcgccccgct ccacaggatg agggccttgg
     4201 gctcctcggc cacccccgcg cggcgttcga ccacgcggca ggggaccccg ttctgtagga
     4261 gcgtgcaggc ggcggccagc cccgtgggac cggcgccgac cacgagcacc ggggggtgcg
     4321 ggcccggcgc gctcatcgcg gagcctccgc ggcccgcgcc ggtcggccgg cgccgtcgag
     4381 cagttccgtg atctcggcga tcgcctgggc gttgtcctgg aaacggacgg tggccatgcc
     4441 cagctcggcg gccggcttga ggttggcctc caggtcgtcc acgaacaggc agcggtcggg
     4501 ggtgaggccg agccgggcca ggagtcgctc gtagatctcg gtgtcgggct tgcggacccc
     4561 ctcgtgcgcg gagttgacca cgacctcgaa gagttcgtcg gcgggcacgg tggcccgcca
     4621 cagctcctcc cactccagga cgttgttggt gagcagggcc agtcggtagc cctccatgtt
     4681 caggtcgcgc aggaagtcgg cgaacccgtg gttgccggtg cgaccggcga accacagttc
     4741 gccgaaggtg cggtcctcgg gcaaggtcca tcccccttcg ggcagctcgg cggtgatctg
     4801 ggcgacgagg tccgcctcgg tgatggcccc gacctccagt agcgccatcg ggtcggcgcc
     4861 gcccttggcc gcggcggcac cgatggcggt gaggatggcg tcggcgctca ggcccgccgt
     4921 cccggcgaag tgctccaagg tctcgtggag cgggttggtg agcacgccgc cgtagtcgca
     4981 cacgacggcc tcgatacggg tcacacccac tgggccacct cgatccggaa ggagctgttg
     5041 gtcgttccct gctggttgac gcggacgtcg acgccgacga ccggaccgaa ccgctcgtcg
     5101 gagcggaagg gcgtgatgtc ggcctccagg gtggagggga ggtcgttctc gcagaaggac
     5161 gagaacgacg cctccaccga gaccgggacc agggtcaccg ggtccaggcc ctcggagcgg
     5221 gccacgccgg ccacggccac ctggcgggcg gcctcgatga ggaggttgcc gggcaggtgg
     5281 tcgagcgggt ggtcgaacag gtgggggtgg ctgtggtcgg ggaccacggt ggcccgccag
     5341 ctctcgtcgc cccgctggac ggggtcggtg atgaccacgt tgcgcggatc gcggcgaccc
     5401 accacggagg gctgggccgg gatcgcccgg ggcgccgcgg cggcgctcca gtccagggac
     5461 tcacggccct tggtgcgcag cgcctggtag gcctggcggc tcaggaagat gagcgcgccg
     5521 gtggcctggg cgacctcggt cccgttgagg aggaccgtgc cgcggaagtc gtagccgcgc
     5581 agtctgcccg cctggttgcg gtacaggtcg gcctcggtgg cgaccatgag gcgggcgggt
     5641 tcggctccct ggcgcagccc gtcgaggtcg ccgatgtgca gcgacatgcg gtgcatgatg
     5701 aacgcccggt ccatggggac gtcgaggtgc cggtgggaga tgagcacgcc ggtctggcgg
     5761 cacgcctcca ggatcagcgg caggtcgtag gtgggggtct ccagcatgac gtggccgcgg
     5821 ggcagctggg cggcgacgtc gaaggtgttc tcgccggtct gccgggagtc ggtgacgaac
     5881 acctccgaga cggccttgcg gtggaccagc ccccggggga cggtcgcgtc gtagtcgagc
     5941 tcgcggatgc cggtgttctc gggatccagg tcggccaggg cccgctgtgc ggagtccgtc
     6001 acctcggcca cggcactcgg ggcgtctgcg gtcgtgcggt tcatgtcttc cctttcgttc
     6061 gcgttcccct cgggggggtg ttccgccgag ggacaacggg gttcggaacg gtgaggagag
     6121 cggtcccccg tgacggggac gggcggggaa cggcccgtcc ccgtacaaga accgagtatc
     6181 gagagggggc ctgaagacga cctggaaccc gacctgaagg ccaattgtga agaaaattca
     6241 tttttgaccc cgcacgcggc gttcacatgc gaagatgcgc gtgcaattga tccctgccgc
     6301 agatgatcaa gactttgtga ctatcgacac aaggtacgac ttgtgccctg tcgcctccgg
     6361 tcctcgtctg cgaaagctcc cgcctcgacg tcggagtgga gccgatccat tacataaagc
     6421 catgatctgc gtgtttatga tcgccttacc gcatccgagc gacatggacc cgtcgggacg
     6481 gggcgggact ccgtacccct cagagcgaat cggccggaac cggaaccggg gcgatgtcgg
     6541 gagaagccgg agtgcacgga acgtgggaga catcgatagc gcaagacaac ggccggaagg
     6601 attccggaat gatcagaaca cggcggacgg caccgacgac cgtctcggtc ctgggagagc
     6661 tggaggtgat cggggatcac ggccctctgc ccctgggcgc accccgacag cgcgcggtgc
     6721 tcgcggccct ggtgctcaac ggcgatcgcc ccttgaccac gcagcgcgtc atcacgatgg
     6781 tctggggttc gacccccccg gcctacgcgg tcaacctggt ccacaagtac atgtcgggcc
     6841 tgcgccgcgc cctgatcgac ctgcccccgc acaagcgggt cgacatcgag agcggaccgt
     6901 cgggataccg gctcgtggca ccggtcggcc aggtggacct ggccctgttc cagacctgga
     6961 tcgacgccgc gcgcgacctg cgcgcacgcg gggacggcga acgggccgcg gagttgttcg
     7021 gccgggcgct ggacctgtgg cgcggccccc tgctcgggga gctctccggc tccgccttcg
     7081 acgaggaacg cgagttcatg gagaccgttc gactggcggt gctggaggag tacgccaccg
     7141 tcgccatcga ggtcggacgc caggccgacg tggtggaatc actgcgcgag gcgctgcgcc
     7201 gccaccccct ggaggagaac ctggccaggc ttctgatcct ggccctgtac agcgagggac
     7261 gcacggccga ggccgtccgt gtgtaccaac gcctgacctc gcacatgctg ggcgagctca
     7321 acgtgccgcc ccgccccgaa ctgcacgatc tctatcagat catccgacgc cacgggccga
     7381 tccccaccgg gagacggctc cagcacatcc cctgaccccc tcctcaccga tgccgccgcg
     7441 caccggtgac catgccgtgg tgcgtcacag gtcccgccgg agagaacctc acggcgggac
     7501 cgtctccgat ctcagactcc ggcgggcacg cgttcgcgtt cgtccgaaac ccggaccgaa
     7561 ggctcctcat ctccgccctc ggagatcccg atgcggtcgt gcagccaccg cagtggccgg
     7621 ggcagccacc agttcacccc gccgagcagg cgcatcgtcg ccggcaccat gatcgcgcgc
     7681 accagagtgg cgtccacgag caccgcgagc gccaggccga tgccgatgat cttcaggaac
     7741 agcaggtccg aggcgcccat ggccaggagc accaccatga gcaggatcgc ggcgctggtg
     7801 atgatccggc cggtgtgctg gagaccggtc gccaccgatc gggtgttgtc accggtgcgc
     7861 aggtactcct cgcggacccg gctgagcagg aacagctcgt agtccatggc caggccgaac
     7921 gcgacgacgg tgatgagcac caggtacgtg ggatcgatcg tgcccaccgc ctcgaacccg
     7981 agcaggcccg cgaaggcccc ctcctggaac ccccagacga ccacgcccag cgaggcgctc
     8041 aaggacagcg cgcccatgag gaccgccttg acggggagga ccaccgagcc gaacgccagg
     8101 aagagcagga acagtgtggc gcccgcgatg aagccgatgg tggtcggggc cgccgccacg
     8161 atggcggcga cgttgtcctg ctgcatggcg gccacaccgc ccaccagggc ctcctccgcg
     8221 ccgtcgggcg cgggttcggc gcgcacgtcg gccacgaggc ccttgatctc gtcggagtcg
     8281 gcggcgccct ggtaggcgac caggacgtgg gcgatgccgt cgccggtgcg ctccacccgt
     8341 gccagggtga cctcgggcag accctccaga cgttcgacgt agtcgtccaa ggcctcgtcg
     8401 ccggcgtcgc cgaccacggc gacgtgcagc cgtcccaggc cgccccccgg gaagtcctcg
     8461 gcgaccgact cggtggcgat ccggctgccg gcgtcctggg gcaggtagcg ctgatcggtg
     8521 gtgcccaggt gggcggagac caggccggag ccgaagatca ccaggacacc gaggaccgcg
     8581 accagtgaga cggcggggcc gcgcatcacg gtgcgggcca gacgcgtcca tccgccctca
     8641 tggagggtcc cggtgacggt gcgtcggggc agcggcagtc tcaggccgtc gatgcggtgg
     8701 cccaccaccg acaacagcgc cggaaggaag accagggcgg cgaggaggtc gaacaggacc
     8761 acggcgatac cgccgaagcc gatcgacttc aggatcggct ggggaaagaa cagcaggccg
     8821 gcgaaggcga tgccgacggt gaccccggag aaggcgacgg tgcgtccggc ggtgtccacg
     8881 gtgcgaccga cggcggcggc caccccgcgc cccctggcca gttcctcgcg gaaccggctg
     8941 accatgaaca ggccgtagtc gatcgccagt cccaggccca ggatggtggc gacgttgacg
     9001 gcgaacaccg acacctcggt gacgtgggtg aggacgcgca gggtcacgag ggagcccagg
     9061 atcgccagac cgcccaccgc gagggggacg acgccggcga tgaggccgcc gaagatcagc
     9121 accagcagga acagcaggag gggcagcgtg atgagttcgg cgcggacgac atcgctctcg
     9181 gcggcctccg acaactcgtg ctcgacggcg atggggccgc ccaggtgggt ctccagcggg
     9241 cccgaggcca ggtgttcggc gacgtcgtcg tactggtcca gccgttcggg gtggctctcc
     9301 cccgtgagcg tgatcgggat gtacgtcgcg tgcatgtcct cggagacgag catgccgcgc
     9361 tcgatgtcgg agagcccttc gtcgaggtag acgtcgaagt cggcgacgac gccccggggc
     9421 atgtcgtcgg cgatgcgctg gatcgccgcg gcgaaggtcg ggttgtccac cttcatctcg
     9481 tcgctgcggt agacggccac gatgtcgacg tcgtcgtggc ccagttcgcc ttcgagcacg
     9541 tccatcgccc gggaggactc ggctccgggg tcctcgaaac cgctttcgct gacgtccgag
     9601 aagacgccgg atccccatac caccgcgaac acggcgaaga ccgcggtggt gaccaggacg
     9661 gcccaccgga agcgatgggc gccaagcccg agacgaccga aaagccccgt gtgcgttggc
     9721 atgagcagcc ctttcggcga acggccttcg tgtccgttaa cgctgttcgc taacggcgtt
     9781 cactaaagcg tgcaggcatt acgatgtcaa tatcaccgga tgtgaccata aaacggggtg
     9841 taccgcagca catgacgaag ccgcagcttg aacgggtcga gcgggtaccg agccgccggg
     9901 agcgtgtgcg cgaggccaca ctctccgaga tcaaggcgat cgcccgcagc cacctcgtcg
     9961 agcacggggt cgcgggagtg tccctgcggg cgatcgcccg agagatgggg atgaccgcgc
    10021 ccggtctgta ccgctacgtg tcggggatcg actcgctcct ggtgctcatc accgccgaca
    10081 tgttcgacga actggccgcc gcggtgtcgg acgccgacgc cagcgtgccc gccgaggaca
    10141 ccgaccggcg catcaacacc tcgctgcgcg ccttccgggg gtgggcgctg gagcacccgg
    10201 cggagttcgc cgtgatgttc gggccgcgca agcggctgga acaggaggtg gacctcactc
    10261 cggcggtcga ggcgggaagt aggttcgggg ccaccttctt caccctgttc gagcgcttga
    10321 tcgacgaggc gaggttcgag ctgcccgaca gcggacgggt caccccggag ctcgcccggg
    10381 agctgcgggc cttcgccgtc acctgcggct tcgcgggatc ggagacgtcc gtcgaggcga
    10441 tgatggtgct cagctcgtgc tgggtgcgcc tgtacggcgt ggtctgcatg gaggtgttcc
    10501 agcacctgga tttcgtgatg cgggacatgg aaccactctt cgagtccgag cttcagaacg
    10561 tactgacagg cctgggcgtt cggtacgaag caccgcaaag caacgaaacg gctaccatca
    10621 gtacgcccga ttaagcccca tactggaggt ctgtggcact tgccggattc gcccggggtg
    10681 gatccggtcc cggccggcgc gccatggcat gagagcgagg accgaccctg tggagaaccg
    10741 acccggcgat gagcggtacg aaccgtccac cggcaccccc tgggtccccg gcaccaccgt
    10801 gcacacgaac tcctcgcggt cctccgacgg cggggccgac cggggccttc cgccgggcgt
    10861 gcccgccgca cccccgcccg ccgccgcccc tcggcccccg gcgacccccg acgcctcggc
    10921 cgcgcccgac ggcgcctcca cccccgggac gccgcagggc ccgtacacat cacaggtcca
    10981 tcgaggcggg ggcgtccccc agggcgccca cgcgcccggt ggatcgcagg gtcacggcgg
    11041 tgtc
//
