LOCUS       NZ_JARBUT010000016     13029 bp    DNA     linear   CON 29-APR-2024
DEFINITION  Nocardiopsis sp. N85 Scaffold16, whole genome shotgun sequence.
ACCESSION   NZ_JARBUT010000016
VERSION     NZ_JARBUT010000016.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nocardiopsis sp. N85
  ORGANISM  Nocardiopsis sp. N85
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsaceae; Nocardiopsis.
REFERENCE   1  (bases 1 to 153433)
  AUTHORS   Liu,S.
  TITLE     Nocardiopsis sp. N85
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 153433)
  AUTHORS   Liu,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Microbial Chemistry, Institute of Medicinal
            Biotechnology, Chinese Academy of Medical Sciences, Tiantanxili No.
            1, Beijing 100050, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 1.05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 182.0x
            Sequencing Technology :: combinatorial probe-anchor synthesis
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028882275.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 06:01:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,663
            Genes (coding)                    :: 5,611
            CDSs (with protein)               :: 5,611
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 46 of 53
            Pseudo Genes (internal stop)      :: 0 of 53
            Pseudo Genes (multiple problems)  :: 3 of 53
            CRISPR Arrays                     :: 13
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:47:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 13029
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JARBUT010000016.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..13029
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene-precursor"
                     /region_number="1"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..13029
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13029
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[1985:3029](-)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(1986..3029)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     misc_feature    complement(55..69)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.51"
                     /tool="antismash"
     gene            complement(66..1925)
                     /locus_tag="PWG71_RS20510"
                     /old_locus_tag="PWG71_20510"
     CDS             complement(66..1925)
                     /EC_number="2.2.1.7"
                     /GO_function="GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA]"
                     /GO_process="GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DXP_synthase_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292341.1"
                     /locus_tag="PWG71_RS20510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_20510"
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="WP_311512157.1"
                     /sec_met_domain="DXP_synthase_N (E-value: 1.8e-85,
                     bitscore: 279.4, seeds: 174, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MALLDTIEGPASLRGLSDERLRELAGEIREFLIDRVTASGGHLGP
                     NLGVVELTIALHTVFDSPKDRLLWDTGHQSYVHKLITGRRAGFDRLRKAGGLSGYPSRA
                     ESEHDLVENSHASTALSYADGLARADALTGTTDRAVVAVIGDGAMTGGMAWEALNNIGG
                     GPDRPLVIVLNDNERSYDPTLGGFADHCARLRGGDADLSVFEQLGLTYLGPVDGHDIPA
                     LREALTRARRTTGPVIVHAVTEKGRGFAPTEENDLDRGHAIRPMDRRTGRALSTSSGPS
                     FSAVFGECVTRIAAERPDVVAVTAAMTEPTGLLPLRERFPERVIDVGIAEQHAVTMAAG
                     LSMGGVHPVVAIYSTFVNRALDQLLMDVALHRRAVTFVLDRSGVTGDDGPSHNGMWDMS
                     VLQVVPGLRICAPRDGARLAELLDEAVSWEEGPTLVRFPKGATGPDIPTETVFAGMDVL
                     YRQGALDVLVVSIGALARVALGVADRLTDLGHGVTVVDPRWVSPVAPGLCDMARRHRLV
                     VTVEDNSRVGGVGSTIAQALRDADVTTPLREFGIPRRFLDHGGRADVLSLCGLTIEDIS
                     MAAAEDMAVLDGRERDPEFAAGLRGLTGGASASTVRSDTEGKV"
     misc_feature    complement(1902..1915)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 18.36"
                     /tool="antismash"
     misc_feature    1903..1916
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 19.39"
                     /tool="antismash"
     gene            complement(1986..3029)
                     /locus_tag="PWG71_RS20515"
                     /old_locus_tag="PWG71_20515"
     CDS             complement(1986..3029)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017623806.1"
                     /locus_tag="PWG71_RS20515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_20515"
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_274811542.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 1.8e-44, bitscore:
                     144.9, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPTVDDVRDLRARLLDRVEDRLDTLLRTERERWARVDPRACVP
                     VDDVAALVAAGGKRLRPAFLLSGHLAAGGDPDDRRAVDCAAAVELVHVGALLHDDVLDD
                     SDLRRGRSTAHVRHLRDHLHHQWRGEARRYGEGVAVLSGDFADILADRLTTELPPDVRT
                     IWNAFLTEIVIGQQLDVAVAARSVVDPELSRWIAVCKSGRYSIHRPLLLGAALAGRSDL
                     APVFERYGESLGEAFQLRDDLIDLFGDSDHTGKPVGLDLETHKMTLLLALAVRRDAEVR
                     ALVEQPEWDVPRLRDRLDALGIGDVIEERIGELVADAVNALRHPSLDSGWRDELAAMAH
                     EVAYRNR"
     gene            complement(3026..4003)
                     /locus_tag="PWG71_RS20520"
                     /old_locus_tag="PWG71_20520"
     CDS             complement(3026..4003)
                     /EC_number="1.17.7.4"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA]"
                     /GO_process="GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF002189.0"
                     /locus_tag="PWG71_RS20520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_20520"
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="WP_274811543.1"
                     /transl_table=11
                     /translation="MQRKKVILAEPRGFCAGVRRAISIIEIALERYGPPVYVRKEIVHN
                     HYVVRVLRERGAIFVDSEDEIPEGEVCVFSAHGVSPEVRRNAGRRRLDVIDATCPLVSK
                     VHQQALRVAKADRTILLIGHSGHEEVEGTHGHAPDRTVIVETVEDARALDLPADAPLAY
                     LTQTTLSVDETREIVEVLRDRFHDLTGPGTDDICYASQNRQDAIKAIAPRSDLVLVVGS
                     RNSSNSVRMVEVARRSGTASHLVPDVGEFSAEWLTGVSTVGVSAGASAPDVLVEELLDL
                     LADHGFRDTELESVAREDIVFSPPTRLARTGPGDDRPASAKGTP"
     misc_feature    complement(4208..4217)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.9"
                     /tool="antismash"
     misc_feature    4227..4241
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.48"
                     /tool="antismash"
     gene            4317..5546
                     /locus_tag="PWG71_RS20525"
                     /old_locus_tag="PWG71_20525"
     CDS             4317..5546
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /locus_tag="PWG71_RS20525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_20525"
                     /product="transposase"
                     /protein_id="WP_274811544.1"
                     /transl_table=11
                     /translation="MNTVIKPVENEIPSPSAAPPPAVADDDPLLNDLVDELFFSLTRSD
                     QRRKGMDYLRGLLSTPGRKSVRNMAAALGGAGTGQSLHHFVCSSTWEWSPVRRALARYT
                     MRESPPQAWVVRPLVIPKVGRHSVGVSRYFSADLGQVVNAQRAVGVWLASDHLSVPVEW
                     RLHLPGRWRGALDCGAPTPAMAQSETLVGCAAEASLAMIESWGLPPRPVILDLDDTDPL
                     PAFRRLYAAGLRPAARIRPELPLRVTDPALIGHRGRTLPAELVMRAGQEMRRPVPDPSD
                     PARRARLVAAVRVTRPSAAPSAPGAAPEEELLLVGIGRGGRPWPQELWLVRPPESHAGP
                     AALWASRLVDRVERSFARFGDPVGMRDYAGRSFNGWHRHITLASVAHTFRALSPVAEAR
                     ARPTTVPAAT"
     misc_feature    4318..4332
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.41"
                     /tool="antismash"
     gene            5662..6831
                     /locus_tag="PWG71_RS20530"
                     /old_locus_tag="PWG71_20530"
     CDS             5662..6831
                     /EC_number="2.5.1.54"
                     /GO_function="GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA]"
                     /GO_process="GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019610420.1"
                     /locus_tag="PWG71_RS20530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_20530"
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="WP_274811545.1"
                     /transl_table=11
                     /translation="MKTESPDLRRRTALQQPDWGDDPRLPEVRERLRARPGLVRIEDVD
                     RLSGLLARVASGEARVVTAGDCAEDPERCGPEAVGAQEFLVGALAEVLEATTGRPVVRV
                     GRIGGQFAKPRSRPTEIVEGRELPVYRGHLVNAPEPTPTGRAHDPERLLACHDAARTVL
                     EHLGRRSGPTGPARTPSLWTAHEALVLDYEVPALRRTPDGRTLLTSTHWPWVGDRTRHP
                     EGAHIALLSGVANPVACKVGPSATVEELLEVCDRLDPERRPGRLTLISRQGVGGTEALR
                     RLVAAVRAAGHPAIWMSDPMHGNTVRTPSGLKTRYVSAIVREVREFQAAVRAAGGIAGG
                     LHLEATHEPVTECVSDASRVDRVADRYTSLCDPRLNPAQAVAVASAWTA"
     gene            6840..7631
                     /locus_tag="PWG71_RS20535"
                     /old_locus_tag="PWG71_20535"
     CDS             6840..7631
                     /EC_number="1.3.1.28"
                     /GO_function="GO:0008667 -
                     2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (11-170). E-value: 7.6e-14.
                     Score: 44.0. Matches aSDomain:
                     nrpspksdomains_PWG71_RS20535_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) EntA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013152075.1"
                     /locus_tag="PWG71_RS20535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_20535"
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase"
                     /protein_id="WP_274811546.1"
                     /sec_met_domain="EntA (E-value: 3e-105, bitscore: 344.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 1.2e-34, bitscore:
                     112.8, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 8.3e-33, bitscore:
                     107.3, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEREESAMERKVALVTGAASGIGAAVARALGERGAMVALVDRDRP
                     GLERVTAGLTDDGVRAVAITADVAAAGEAERVVAEAEDLLGSLDHLVNAAGVLRMGGAW
                     ELDDRQWNESLEVNATGVFAMSRAAARTMVTRSTGAIVTVASNAAGTARAGMAAYAASK
                     AAAVAFTRCLGLELAGHGVRCNVVAPGSTDTPMLRSMWKDAAGPRATLDGDPDAYRVGI
                     PLRRIAEPDDVADAVVFLLSDRARHITMQTLTVDGGATLGA"
     aSDomain        6873..7349
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PWG71_RS20535_PKS_KR.1"
                     /evalue="7.60E-14"
                     /label="PWG71_RS20535_PKS_KR.1"
                     /locus_tag="PWG71_RS20535"
                     /protein_end="170"
                     /protein_start="11"
                     /score="44.0"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VALVTGAASGIGAAVARALGERGAMVALVDRDRPGLERVTAGLTD
                     DGVRAVAITADVAAAGEAERVVAEAEDLLGSLDHLVNAAGVLRMGGAWELDDRQWNESL
                     EVNATGVFAMSRAAARTMVTRSTGAIVTVASNAAGTARAGMAAYAASKAAAVAFT"
     CDS_motif       6879..6944
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PWG71_RS20535_0001"
                     /evalue="1.20E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="PWG71_RS20535"
                     /protein_end="35"
                     /protein_start="13"
                     /score="18.0"
                     /tool="antismash"
                     /translation="LVTGAASGIGAAVARALGERGA"
     gene            7683..8366
                     /locus_tag="PWG71_RS20540"
                     /old_locus_tag="PWG71_20540"
     CDS             7683..8366
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027: isochorismatase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019610300.1"
                     /locus_tag="PWG71_RS20540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_20540"
                     /product="isochorismatase family protein"
                     /protein_id="WP_274811547.1"
                     /transl_table=11
                     /translation="MPGIPPIRNYPMPTADALPDNVARWDLDPDRAVLLVHDMQRFFLR
                     PFATTEEPYGTLLKNCALLREHAVRAGARVAYTAQPGRMTPGERGLLRDFWGPGMTTSV
                     EDREVVEPLTPGPDDWMFTKWRYSAFHRSDLLERMRAEGRDQLVLCGVYAHVGVLMTGV
                     DAFSHDIRVFLAADAVADFDAEHHRLALEYAATRCAVVAPTERLLDRMTVPGADRSPVE
                     VEEER"
     gene            8363..10252
                     /locus_tag="PWG71_RS20545"
                     /old_locus_tag="PWG71_20545"
     CDS             8363..10252
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1018: isochorismate synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019610301.1"
                     /locus_tag="PWG71_RS20545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_20545"
                     /product="anthranilate synthase family protein"
                     /protein_id="WP_274811548.1"
                     /transl_table=11
                     /translation="MTAHPHDTDSGNLLDRIVTGAVPAFALVHRPESGPGAIEVLTGTV
                     SLHDSLADIPLEEGGDAPGPPGWDVLVAVPYRQISERGFASVDDGAPIISLKVSECREE
                     TVERVLERVPDTGLTVNGGDFDIDDDTYSDTVRRVITEEIGRGEGSNFVLRRSFTARIE
                     GFGHGSALSLFRRLLEQESGTYWTFVLFTPEYTLVGATPERHVSLEGGTAVMNPISGTY
                     RYPAGGPTLNGMVDFLADRKEADELYMVVDEELKMMARICDSGLRVVGPRLKEMARLAH
                     TEYLIQGECDRDPREILRETMFAPTVTGSPLENACRVIARYETLGRGHYSGALALIGRD
                     ARGAAALDSAILIRTAEIDRGGHLRLSVGATLVRHSVPESEVVETRAKAAGLLSALNAD
                     RSKALAGDPTVLDALAARNARLSGFWLGRPCPTPQVTLDGMDILVVDAEDTFTAMLAHQ
                     LRSCGASVRVRGFAEEFAPDGYDLVIMGPGPGDPRDLDHPKIARVRAGVRTLLARRSPF
                     LAVCLSHQALSLELGLPVLRREHPNQGAQHLIDLFGHQELVGFYNTFSTHCEEDKIHCE
                     GLGAVEVSRDPGTGEVHALRGPWFASVQFHTESVLTRDGVGVITRLITEVAGR"
     gene            complement(10280..11596)
                     /locus_tag="PWG71_RS20550"
                     /old_locus_tag="PWG71_20550"
     CDS             complement(10280..11596)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017556324.1"
                     /locus_tag="PWG71_RS20550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_20550"
                     /product="NDP-hexose 2,3-dehydratase family protein"
                     /protein_id="WP_274811549.1"
                     /transl_table=11
                     /translation="MRGPDGAPTSDGPADVVAWAAERAAAGGFSVTRVPFAEMDSWGFR
                     PDTGDLVHDSGRFFSITGLRSVVDGDPGTLRHQPVIHQPEVGVLGLLAKEFDGVLHFLV
                     QAKMEPGNPGIVQLSPTVQATRSNYTRAHRGRPVRHIEHFVGSGRGRVLTDVLQSEHGW
                     WFHHKSNRNMIVETDREVEPHEDFRWVTLGTLGALMHVDETVNMDTRTVLACAPVSFGA
                     DGSAHSDTGLLSWFTEQRASRTLSAERVPLGSLPGWVRTPDRLHHEQDRDFDVVAVAVR
                     ARAREVASWSQPLLAPRGPGVAAFLTRRVAGVPHLLVHARVEPGFLDTVELGPTVQCTH
                     DEATAPPGLRPPFLDTVLSAPPELVRYRAVHSEEGGRFLHARTRCLVVEDPRPAGAPEP
                     PGYLWATPDQLVALARHGHYLDVQARTLLAALTTGALRP"
     gene            complement(11652..12605)
                     /gene="rfbB"
                     /locus_tag="PWG71_RS20555"
                     /old_locus_tag="PWG71_20555"
     CDS             complement(11652..12605)
                     /EC_number="4.2.1.46"
                     /GO_function="GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (4-200). E-value: 1e-17.
                     Score: 56.0. Matches aSDomain:
                     nrpspksdomains_PWG71_RS20555_NAD_binding_4.1"
                     /codon_start=1
                     /gene="rfbB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017601707.1"
                     /locus_tag="PWG71_RS20555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_20555"
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="WP_274811550.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 1e-20, bitscore:
                     66.9, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 3.8e-20,
                     bitscore: 65.1, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 2.1e-17, bitscore:
                     56.0, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRILVTGAAGFIGSHYVRSLLADADAPRVTALDLLTYAGNRDNLP
                     PSHPRMEFVRADVCDARALADVLPGHDAVVHFAAESHVDRSVADGSVFVRTNVVGTQTL
                     LAAAVAAGVERVVHVSTDEVYGSLERGAAREDHPLAPNSPYAASKAGSDLIARAYWRTH
                     GLDVSVTRCSNNYGPRQHPEKLIPRFVTALVSGGRVPLYGDGRNVREWLHVEDHCRAVH
                     LVLTRGRAGGVYNVGGGDGLTNLEMTRRLLELCGADETAIERVPDRLGHDLRYCIDDTT
                     IREELGYEPRVPFARGLRDTVAWYRDNPGWWKPLAA"
     aSDomain        complement(12006..12593)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PWG71_RS20555_NAD_binding_4.1"
                     /evalue="1.00E-17"
                     /label="PWG71_RS20555_NAD_binding_4.1"
                     /locus_tag="PWG71_RS20555"
                     /protein_end="200"
                     /protein_start="4"
                     /score="56.0"
                     /tool="antismash"
                     /translation="VTGAAGFIGSHYVRSLLADADAPRVTALDLLTYAGNRDNLPPSHP
                     RMEFVRADVCDARALADVLPGHDAVVHFAAESHVDRSVADGSVFVRTNVVGTQTLLAAA
                     VAAGVERVVHVSTDEVYGSLERGAAREDHPLAPNSPYAASKAGSDLIARAYWRTHGLDV
                     SVTRCSNNYGPRQHPEKLIPRFVTALVSGGRVP"
ORIGIN
        1 ccgggtcttg cgccgcgtcg ccagcgggcg cggcggagtg gcgggaatgc cgagatcgac
       61 ggtggtcata ccttcccctc ggtgtcggaa cgaacggtgg aggccgatgc gccgccggtg
      121 aggccgcgca gaccggccgc gaactccggg tccctttcgc ggccgtccag cacggccatg
      181 tcctcggccg ccgccatcga gatgtcctcg atggtcagcc cgcacaggga gagcacgtcg
      241 gcgcggcctc cgtggtccag gaagcggcgg gggatgccga actcgcgcag cggtgtggtg
      301 acatcggcgt cgcgcagcgc ctgggcgatg gtggaaccca ccccgcccac ccggctgttg
      361 tcctccacag tgaccaccag gcggtgacgg cgggccatgt cgcacagacc gggggcgacc
      421 ggcgagaccc agcgcgggtc cacgaccgtc acgccgtggc ccaggtcggt gaggcggtcg
      481 gcgacgccca gggccacgcg cgccagcgcg ccgatcgaca ccaccaggac gtccagggcg
      541 ccctggcggt agaggacgtc catcccggcg aacacggtct cggtggggat gtcgggtccg
      601 gtggccccct tggggaagcg caccagggtg ggcccctcct cccaggagac ggcctcgtcc
      661 aggagttcgg ccagcctggc cccgtcgcgg ggggcgcaga tgcgcaggcc cggcaccacc
      721 tggaggacgg acatgtccca catgccgttg tggctgggcc cgtcgtcacc ggtgaccccc
      781 gacctgtcca ggacgaaggt gacggcccga cggtgcaggg ccacgtccat gaggagctgg
      841 tccagggcgc ggttgacgaa ggtggagtag atggccacca ccgggtggac gccgcccatg
      901 gacagcccgg ccgccatcgt caccgcgtgc tgttccgcta tgccgacgtc gatgacgcgt
      961 tcggggaagc gctcgcgcaa cggcagcagg ccggtgggct cggtcatcgc cgcggtgacg
     1021 gccaccacgt cggggcgctc ggcggcgata cgggtgacgc actcgccgaa caccgcgctg
     1081 aaggaggggc cggaggacgt ggacagggct cgtccggtcc gacggtccat ggggcggatg
     1141 gcgtgccccc ggtccaggtc gttctcctcc gtgggggcga acccccgccc cttctcggtg
     1201 acggcgtgca cgatcaccgg accggtcgtc cgcctggcgc gggtgagcgc ctcccgcagg
     1261 gcgggaatgt catggccgtc caccggcccc aggtaggtca gccccagctg ctcgaacacg
     1321 ctgaggtcgg cgtcgccgcc gcgcaggcgc gcgcaatggt cggcgaagcc gccgagggtg
     1381 gggtcgtagg agcgctcgtt gtcgttgagc acgatgacca gggggcggtc ggggccgcca
     1441 ccgatgttgt tgagcgcctc ccaggccatc ccgccggtca tggccccgtc gccgatgacg
     1501 gcgaccaccg cccggtccgt ggtcccggtg agggcgtcgg cgcgggccag accgtcggcg
     1561 taggacagcg cggtcgaggc gtgggagttc tccaccaggt cgtgctcgga ctcggcacgg
     1621 gaggggtagc cggacaggcc gcccgccttg cggagccggt cgaacccggc ccggcgcccc
     1681 gtgatcagct tgtggacgta ggactggtgc ccggtgtccc agagcagccg gtccttcggg
     1741 gagtcgaaga ccgtgtgcag ggcgatggtc agttccacca cccccaggtt ggggcccagg
     1801 tgaccaccgg aggcggtgac ccggtcgatg aggaattcgc ggatctcccc ggcgagttcc
     1861 cggagccgtt cgtcggagag cccgcgcaac gacgcgggtc cttcgatcgt gtccaacagg
     1921 gccatgtgat cactcctcgg gaccgcctcg gggcggtccg ggttacggcg gtgggcggca
     1981 cccgttcagc ggttgcggta ggcgacctcg tgcgccatgg cggcgagttc gtcacgccac
     2041 ccggagtcca gggacgggtg gcgcagcgcg ttgacggcgt cggcgaccag ttcgccgatg
     2101 cgctcctcga tgacgtcgcc gatgcccagg gcgtcgagcc ggtcgcgcag tcggggcacg
     2161 tcccattcgg gctgctccac cagcgcgcgc acctcggcgt cgcgccgcac ggccaaggcc
     2221 aggagcaggg tcatcttgtg ggtctccagg tcgaggccga ccggtttccc ggtgtggtcg
     2281 ctgtccccga acaggtcgat gaggtcgtcc cggagctgga acgcctcgcc cagggactcc
     2341 ccgtacctct cgaagaccgg tgccaggtcg gagcgcccgg ccagggcggc gcccagcagc
     2401 agggggcggt ggatgctgta ccggcccgac ttgcagacgg cgatccaccg ggacagctcg
     2461 ggatcgacga ccgagcgggc cgccacggcg acgtccaatt gctggccgat gacgatctcg
     2521 gtgaggaagg cgttccagat cgtgcgcacg tcgggcggca gctcggtggt gagccggtcg
     2581 gccaggatgt cggcgaagtc cccggagagc acggccaccc cctcgccgta gcggcgggcc
     2641 tcgcccctcc actggtggtg gaggtggtcg cgcaggtggc gcacgtgggc ggtggaacgc
     2701 ccccgacgca ggtcggagtc gtccaggacg tcgtcgtgca ggagcgcacc gacgtggacg
     2761 agttcgacgg cggcggcgca gtcgacggcc cgccggtcgt cggggtcccc gcccgcggcc
     2821 aggtggccgc tgagcaggaa ggcggggcgc aggcgcttgc cgcccgcggc gaccagggcg
     2881 gccacgtcgt ccaccggcac acaggcgcgg gggtcgaccc tcgcccaccg ttcgcgttcg
     2941 gtgcgcagca gggtgtcgag ccggtcctcg acccggtcca gcagtcgggc gcgcaggtcc
     3001 cggacgtcat cgacggtggg tgtcgtcatg gggtcccctt cgcggatgcg ggccggtcgt
     3061 cgccgggccc ggtgcgggcg agtctcgtcg gcggggagaa gacgatgtcc tcccgggcga
     3121 cgctctccag ctcggtgtcg cggaagccgt ggtcggcgag gaggtcgagc agttcctcga
     3181 ccagcacatc gggggcgctg gccccggcgc tgacccccac cgtcgagacc ccggtgagcc
     3241 actccgcgga gaattcgccc acgtcgggta ccaggtggga ggcggttccg gaacgcctgg
     3301 ccacctcgac catgcgcacg gagttgctgg agttgcgcga gcccaccacc aggacgagat
     3361 cgctgcgggg ggcgatggcc ttgatcgcgt cctggcggtt ctggctggcg tagcagatgt
     3421 catcggtgcc cggcccggtg aggtcgtgga accgatcgcg gagcacctcg acgatctctc
     3481 gggtctcgtc gaccgacagg gtggtctggg tgagataggc cagcggggcg tcggccggca
     3541 ggtccagggc ccgggcgtcc tccaccgtct ccacgatcac ggtccggtcg ggggcgtgcc
     3601 cgtgggtgcc ctcgacctcc tcgtgcccgg agtgtccgat gagcaggatc gtccggtccg
     3661 ccttggcgac gcgcagggcc tgctggtgga ccttggacac cagggggcag gtggcgtcga
     3721 tgacgtccag ccgtcgcctc ccggcgttgc ggcgcacctc gggcgagacg ccgtgcgcgg
     3781 agaagacgca gacctctccc tcggggatct cgtcctccga atccacgaag atcgcgccgc
     3841 gctcgcgcag cacgcgcacc acgtagtggt tgtggacgat ctccttgcgc acgtagaccg
     3901 gtgggccgta ccgctccaag gcgatctcga tgatggatat cgctcgccgg acacctgcgc
     3961 aaaaacctcg cggctcggca aggatcactt tctttcgctg cacctcaacg ccacctccag
     4021 tgacccggct ccggcgtttc cggacaccgg gagggtcgcc ctccggtgtt atccatgact
     4081 tcgttcgcgc tcccccgact ctgcaaccgg agccggcgcc gggtcaacac gggagcagga
     4141 gaaatagcag gaatcagcta catgtgacca gaacctgctg gacaggccat tgtcagtcgc
     4201 ttgtcattca caaaacgacg aatccgttct tagtgtgaga gccacctcag aaagaaatcg
     4261 gaaaacacat tagccccgtg tgggccgagc gaagtgcacc ggagcggaga atccgcgtga
     4321 ataccgtgat caaacccgtg gagaacgaga tcccgtcacc gtccgccgcc ccgccgcccg
     4381 cagtggcgga cgacgacccg ttgctgaacg acctggtcga cgagttgttc ttttccctga
     4441 cccgcagcga ccaacgccgt aaagggatgg actacctgcg cggactgttg agcacccccg
     4501 gccgcaagtc ggtccggaac atggcggccg ccctcggcgg cgccgggacg gggcagagcc
     4561 tgcaccactt cgtgtgcagt tccacctggg agtggtcgcc ggtgcgccgg gccctggccc
     4621 ggtacacgat gcgcgagtcg ccgccgcagg cgtgggtggt acgccccctg gtcatcccga
     4681 aggtcgggcg gcactcggtg ggcgtgagcc ggtacttctc cgcggacctg ggacaggtcg
     4741 tgaacgccca gcgcgccgtg ggggtgtggc tggcctccga tcacctgagc gtgccggtgg
     4801 agtggcggct gcaccttccc ggccggtggc ggggcgcgct ggactgcggc gcccccactc
     4861 cggcgatggc ccagtcggag accctggtgg ggtgcgccgc cgaggcgagc ctcgccatga
     4921 tcgagagctg ggggctgccc cctcgcccgg tgatcctgga cctggacgac accgacccgt
     4981 tgcccgcgtt ccgtcgcctg tacgcggccg ggctgcgccc ggcggcgcgg atccgaccgg
     5041 agctgccgct gcgggtgacc gaccccgccc tcatcggcca ccgggggcgg acactgcccg
     5101 ccgagctggt gatgcgggcc ggccaggaga tgcgccgacc ggtaccggac ccctcggacc
     5161 ccgcccggcg ggcccgactg gtcgcggcgg tgcgggtgac ccgcccctcg gccgcgccgt
     5221 cggcgcccgg ggccgctccc gaggaggagt tgttgctggt cgggatcggc cggggcggac
     5281 gcccctggcc ccaggaactg tggctggtcc ggccaccgga gtcccacgcg ggccccgccg
     5341 ccctgtgggc gagccggctg gtggaccggg tcgagcggtc cttcgcgcgg ttcggcgacc
     5401 cggtgggcat gcgcgactac gcgggccggt cgttcaacgg gtggcaccgc cacatcacac
     5461 tcgcctcggt cgcgcacacc ttccgcgccc tgtcgccggt cgcggaggcg cgggcacggc
     5521 ccacgaccgt ccccgccgcg acatgacgaa gtacagccga cttcccatgg ccgagcccga
     5581 gacctcggca cactcgaacg gtccgcggta cgcgagcgac cctctcgtgc cgcaccgacg
     5641 cacccggatc atggagcagc cgtgaagacc gagtcgcccg acctgcgccg acgaaccgcg
     5701 ctccagcagc ccgactgggg cgacgacccc cggttgcccg aggtccgcga acggctccgg
     5761 gcccggcccg gactggtgcg gatcgaggac gtggaccggt tgagcggcct gctggcgcgg
     5821 gtcgcgtcgg gcgaggcccg tgtggtcacc gccggcgact gcgccgagga cccggagcgg
     5881 tgcgggcccg aggcggtcgg cgcgcaggag ttcctggtgg gcgccctggc cgaggtgctg
     5941 gaggcgacga cgggtcggcc ggtggtgcgg gtgggccgga tcggcggaca gttcgccaaa
     6001 ccgcgctcgc ggcccaccga gatcgtcgag gggcgcgaac tgccggtgta ccggggccat
     6061 ctggtcaacg ccccggaacc gaccccgacc gggcgcgcgc acgaccccga gcgtctgttg
     6121 gcgtgccacg acgcggcgcg caccgtgctg gagcacctgg gccggcggtc cggtcccacc
     6181 ggcccggccc ggacgccgtc gctgtggacg gcgcacgagg ccctggtgct cgactacgag
     6241 gtccccgcgc tgcgccgcac cccggacggg cgcaccctgc tgacctccac gcactggccg
     6301 tgggtgggag accgcacccg ccaccccgag ggggcgcaca tcgccctgtt gtccggtgtc
     6361 gccaacccgg tggcctgcaa ggtcggcccc tcggcgaccg tcgaggagtt gctggaggtg
     6421 tgcgaccggc tggaccccga gcggcggccg gggcggctca ccctcatctc ccgccagggg
     6481 gtgggcggaa cggaggcgct gcgccgcctg gtcgcggccg tgcgcgccgc gggccacccg
     6541 gcgatctgga tgagcgaccc gatgcacggc aacaccgtca gaacgccctc gggtctgaag
     6601 acccgctacg tgtcggcgat cgtccgggag gtgcgggagt tccaggcggc ggtgcgcgcg
     6661 gccggcggca tcgccggggg cctgcacctg gaggccaccc acgagccggt caccgagtgc
     6721 gtgtccgacg cctcccgggt cgaccgggtg gccgaccggt acaccagcct gtgcgatccc
     6781 cggctcaacc cggcccaggc ggtcgcggtg gcgtcggcgt ggaccgcgtg aggaccccca
     6841 tggaacgaga ggagtccgcg atggagcgga aggtggccct ggtgaccggg gccgcgagcg
     6901 gtatcggggc ggccgtggca cgcgctctgg gcgagcgggg cgcgatggtg gccctggtgg
     6961 accgggaccg ccccggcctg gagcgggtca ccgccgggct cacggacgac ggggtgcggg
     7021 cggtggcgat cacggcggac gtggccgccg ccggggaggc cgagcgggtg gtcgccgagg
     7081 ccgaggacct gctgggatca ctggaccacc tggtcaacgc cgccggggtg ctgaggatgg
     7141 gcggggcctg ggagctcgac gaccggcagt ggaacgagag cctggaggtg aacgccaccg
     7201 gtgtcttcgc catgtcccgg gcggcggcgc gcacgatggt gacgcgttcg accggggcca
     7261 tcgtcaccgt ggcctccaac gcggcgggca ccgcgcgtgc cggcatggcc gcctacgccg
     7321 cctcaaaggc cgcggccgtg gcgttcaccc gctgcctggg gctggagctg gccggccatg
     7381 gcgtgcgctg caacgtcgtg gccccgggct ccaccgacac cccgatgctg cggtcgatgt
     7441 ggaaggacgc ggcgggcccc cgggccaccc tcgacggcga ccccgacgcc taccgggtgg
     7501 gcatacccct gcgtcggatc gccgagcccg acgacgtcgc cgacgcggtc gtcttcctac
     7561 tgtccgaccg ggcccggcac atcaccatgc agacgctcac cgtggacggt ggggccaccc
     7621 tgggcgcgtg aacccgcccg caccggggac cgcaccggac gaccagcagg aggaggacga
     7681 ccatgccagg catcccgccc atccggaact accccatgcc gaccgccgac gcactgcccg
     7741 acaacgtcgc gcggtgggac ctcgatcccg accgagcggt cctgctcgtc catgacatgc
     7801 agcggttctt cctccggccc ttcgccacca ccgaggagcc gtacggaacg ctgctgaaga
     7861 actgtgccct gttgcgcgag cacgccgtgc gtgcgggcgc acgggtcgcg tacaccgccc
     7921 agccgggacg gatgaccccc ggcgaacgcg gcctgctccg cgatttctgg ggaccgggca
     7981 tgaccacttc cgtcgaggac cgcgaggtgg tggaaccgct caccccggga ccggacgact
     8041 ggatgttcac caagtggcgt tacagcgcct tccaccggtc cgacctgctg gagcgcatgc
     8101 gcgccgaggg gcgcgaccag ttggtgctct gtggcgtgta cgcgcacgtg ggcgtgttga
     8161 tgacgggggt ggacgccttc agccatgaca tccgcgtgtt cctggcggcc gacgcggtcg
     8221 ccgacttcga cgccgaacac caccgtctcg cgctggagta cgccgcgacg cggtgcgccg
     8281 tggtggcgcc caccgagaga ctgctcgacc gcatgaccgt ccccggggcc gaccggagcc
     8341 cggtcgaagt ggaggaggaa cgatgaccgc acacccccac gacacggatt ccgggaacct
     8401 gctcgaccgg atcgtcaccg gcgcggtgcc cgcgttcgca ctggtgcacc gtcccgagtc
     8461 cggcccgggg gcgatcgagg tgctgaccgg gacggtgtcc ctgcacgact ccctcgccga
     8521 catcccgctg gaggagggcg gcgacgcgcc cggtcccccc gggtgggacg tactggtggc
     8581 cgtaccgtac cggcagatct cggagcgggg gttcgcgagc gtcgacgacg gcgcgccgat
     8641 catctcgctg aaggtctccg agtgccgcga ggagaccgtg gagcgggtgt tggagcgggt
     8701 ccccgacacc gggctgacgg tgaacggcgg cgacttcgac atcgacgacg acacctattc
     8761 cgacaccgtg cggcgggtca tcaccgagga gatcggccgc ggcgagggct ccaacttcgt
     8821 gctgcgccgt tcgttcaccg cgcggatcga ggggttcggc cacggcagcg ccctgagcct
     8881 gttccgtcgc ctcctggagc aggagtcggg gacctactgg acgttcgtgc tgttcacccc
     8941 cgagtacacc ctggtgggcg ccacccccga gcggcacgtc agcctcgaag gcgggacggc
     9001 ggtgatgaac cccatcagcg gcacctaccg gtacccggcg ggaggcccca ccctgaacgg
     9061 gatggtggac ttcctggccg accgcaagga ggccgacgag ctgtacatgg tggtggacga
     9121 ggaactgaag atgatggccc ggatctgcga ctccgggctg cgcgtggtgg gtccccggct
     9181 caaggagatg gcccggctgg cgcacaccga atacctgatc cagggggaat gcgaccggga
     9241 cccgcgcgag atcctgcgcg agacgatgtt cgcgcccacc gtcaccggca gcccgctgga
     9301 gaacgcctgc cgggtgatcg cgcgttacga gaccctgggg cggggccact acagcggggc
     9361 gctggcgctc atcggccgcg acgcccgcgg ggccgccgcc ctggattcgg cgatcctcat
     9421 ccgcaccgcg gagatcgacc gggggggtca tctgcgcctg tcggtggggg cgaccctggt
     9481 ccgccactcc gtcccggagt cggaggtggt ggagacccgg gcgaaggcgg ccgggctgct
     9541 gagcgcgctg aacgccgacc gttcgaaggc cctggccggc gaccccacgg tgctggacgc
     9601 gctggccgcg cgcaacgcgc gcctgtcggg tttctggctg ggccggccct gccccacccc
     9661 ccaggtgacc ctggacggga tggacatcct ggtcgtcgac gccgaggaca cgttcaccgc
     9721 catgctcgcc caccagctgc gctcgtgcgg ggcgtcggtg cgggtgcgcg ggttcgccga
     9781 ggagttcgcc ccggacgggt acgacctggt gatcatgggg ccgggcccgg gtgatccgcg
     9841 cgacctggac caccccaaga tcgcccgggt ccgggcgggg gtgcggaccc tgctggcacg
     9901 acggtcgccg ttcctcgccg tgtgcctgag ccatcaggcc ctcagcctgg aactgggcct
     9961 gccggtactg cgccgggagc accccaacca gggggcgcag cacctgatcg acctgttcgg
    10021 ccaccaggag ctggtgggct tctacaacac cttctcgacc cactgcgagg aggacaagat
    10081 ccactgcgag ggcctggggg cggtcgaggt gagccgcgac cccggcaccg gggaggtgca
    10141 cgcgctgcgc ggaccgtggt tcgcctcggt gcagttccac accgagtcgg tgctcacccg
    10201 cgacggggtg ggggtcatca cccgcctgat caccgaggtg gcggggcgct gaggcccacc
    10261 ggggccgccg cggccgtcct cacgggcgca gcgccccggt ggtgagcgcg gccagcaggg
    10321 tgcgggcctg gacgtccagg tagtggccgt gacgggccag cgccacgagc tggtcggggg
    10381 tggcccacag gtaccccggg ggctcgggcg ccccggcggg gcgggggtcc tcgaccacca
    10441 ggcaacgggt gcgggcgtgc aggaaccggc cgccctcctc ggagtggacg gcacggtagc
    10501 gcaccagttc gggcggggcg gacagcaccg tgtccaggaa gggcggccgc agccccgggg
    10561 gcgcggtcgc ctcgtcgtgg gtgcactgca cggtcggccc cagctcgacc gtgtccagga
    10621 agcccggctc gacccgggcg tggacgagca ggtgcgggac cccggccacc cgacgggtca
    10681 ggaaggccgc cacgcccggc ccgcgcgggg ccagcagggg ctgggaccag gaggcgacct
    10741 cgcgggcccg ggcccggacc gccacggcca ccacgtcgaa gtcgcggtcc tgttcgtggt
    10801 gcagccggtc gggggtgcgc acccagccgg gcagggagcc cagggggacc cgttcggcgc
    10861 tcagggtcct cgacgcgcgt tgctcggtga accacgacag gagtccggtg tcggagtggg
    10921 cggatccgtc ggcgccgaag gacaccgggg cgcaggccag aaccgtgcgg gtgtccatgt
    10981 tgacggtctc gtcgacgtgc atcaacgcgc cgagggtgcc cagcgtgacc cagcggaagt
    11041 cctcgtgggg ctcgacctcg cggtcggtct ccacgatcat gttccggttg ctcttgtggt
    11101 ggaaccacca gccgtgctcg gactggagga cgtcggtgag cacgcggccc cgacccgacc
    11161 cgacgaagtg ctcgatgtgg cgcaccgggc gcccccggtg cgcgcgggtg tagttgctgc
    11221 gggtggcctg gacggtcggt gagagctgga cgatgcccgg gttgccgggc tccatcttgg
    11281 cctgcaccag gaagtgcagg accccgtcga actccttcgc gagcaggccc aggacgccca
    11341 cctcgggctg gtggatgacg ggctggtggc ggagggtgcc gggatcgccg tccacgacgc
    11401 tgcgcaggcc ggtgatggag aagaaccgtc cgctgtcgtg gacgaggtcg ccggtgtcgg
    11461 gccgaaagcc ccaggagtcc atctcggcga agggcacccg ggtgacggag aacccgccgg
    11521 cggcggcgcg ttcggcggcc caggcgacga cgtcggccgg gccgtcggag gtgggggcgc
    11581 cgtcggggcc ccgcaccgac ggcacgtcgg cggagagcgc cagccgctcg gcgtcgtgcc
    11641 ggccggaggg gtcaggccgc gaggggtttc caccagccgg ggttgtcgcg gtaccaggcg
    11701 accgtgtcgc gcaggcctcg ggcgaagggg acgcggggtt cataccccaa ctcctcacgg
    11761 atcgtggtgt cgtcgatgca gtagcgcaga tcgtgcccga gccgatcggg aacccgttcg
    11821 atggccgtct cgtcggcgcc gcacagctcc agcaggcgcc gggtcatctc caggttggtc
    11881 agaccgtcgc cgccgccgac gttgtagacc ccgcccgccc gaccccgggt cagcaccagg
    11941 tggacggccc ggcagtggtc ctccacgtgc agccactcgc ggacgttgcg gccgtcgccg
    12001 tagaggggga cgcgaccgcc cgacaccagg gcggtgacga acctggggat gagcttctcg
    12061 gggtgctggc gcggcccgta gttgttggag caccgggtga cggagacgtc gaggccgtgg
    12121 gtgcgccagt aggcgcgggc gatgaggtcc gaccccgctt tggaggccgc gtacggggag
    12181 ttgggggcga gcgggtggtc ctcgcgcgcc gcaccccgtt ccagggaacc gtagacctcg
    12241 tcggtggaca cgtgcacgac ccgttcgaca ccggccgcca ccgccgccgc caacagggtc
    12301 tgggtgccga cgacgttggt gcggacgaac accgacccgt cggcgacgga gcggtccacg
    12361 tggctctcgg ccgcgaagtg caccacggcg tcgtgtccgg ggagcacgtc ggccagggcc
    12421 cgggcgtcgc agacgtcggc ccggacgaac tccatgcgcg ggtgggaggg cggcaggttg
    12481 tcccggttgc ccgcgtaggt gagcaggtcc agggcggtga cccggggcgc gtcggcgtcc
    12541 gccagcaggg accggacgta gtgcgatccg atgaacccgg cggcgccggt gaccaggatc
    12601 ctcatatccg gacctccgcc cggccgtggt cgccgatcac gaggctgggg ccgagggccg
    12661 aggtgtccgt gggccggacc gaggcggatc tgccgatgat ggaactgtgc aggccgggca
    12721 ctccgaccag ttcggcgtcg tccaggacga tggagtcgcc gatcccggac cgtcggatga
    12781 cgcagtccct gcccacggag gtgtgcggtc ccacgtggcc gtcctcgatg acggtgcggg
    12841 cgcccacgat caccggaccg tcgatgcgcg aacgcacgat ccgcgctccg ggctccacca
    12901 ccacggggcc gttcagcacg ctggactcgt ccacccaccc ccggatctct ctccgcgtcc
    12961 cgtccagggc ccagcggttg cagcgcagga cgcccgggat gtcccccgcg tcctgccaga
    13021 acccggtgt
//
