LOCUS       NZ_JARBUT010000014     21206 bp    DNA     linear   CON 29-APR-2024
DEFINITION  Nocardiopsis sp. N85 Scaffold14, whole genome shotgun sequence.
ACCESSION   NZ_JARBUT010000014
VERSION     NZ_JARBUT010000014.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nocardiopsis sp. N85
  ORGANISM  Nocardiopsis sp. N85
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsaceae; Nocardiopsis.
REFERENCE   1  (bases 1 to 171589)
  AUTHORS   Liu,S.
  TITLE     Nocardiopsis sp. N85
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 171589)
  AUTHORS   Liu,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Microbial Chemistry, Institute of Medicinal
            Biotechnology, Chinese Academy of Medical Sciences, Tiantanxili No.
            1, Beijing 100050, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 1.05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 182.0x
            Sequencing Technology :: combinatorial probe-anchor synthesis
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028882275.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 06:01:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,663
            Genes (coding)                    :: 5,611
            CDSs (with protein)               :: 5,611
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 46 of 53
            Pseudo Genes (internal stop)      :: 0 of 53
            Pseudo Genes (multiple problems)  :: 3 of 53
            CRISPR Arrays                     :: 13
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:47:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 37592
            Orig. end                         :: 58798
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JARBUT010000014.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21206
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="1"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..21206
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21206
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11206](+)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11206
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     misc_feature    complement(615..629)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.9"
                     /tool="antismash"
     misc_feature    complement(1670..1684)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.18"
                     /tool="antismash"
     gene            2145..3464
                     /locus_tag="PWG71_RS19310"
                     /old_locus_tag="PWG71_19310"
     CDS             2145..3464
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /locus_tag="PWG71_RS19310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_19310"
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="WP_274811280.1"
                     /transl_table=11
                     /translation="MTTSRTVTVIAVAFATVVSGCSSMPGGEPGEARSGSEGLHEVAEH
                     PLEERAVPGSISESAWTWEGPRAVGVEEVLPVPTGAVLRLTDGVVALDPVTGEEVWTFR
                     LPGAEADAATSPDGAAVAVIAQGTLVLLNSSTGEELDTREYDTDGGSGLELSGVGLVAD
                     TGMVAATEDPGSGLEVRLRPWAEQDEGWTAGPLLCADGSAGTGIEDGFLTPTGVVVVFG
                     CSGGTSAMVSLDASSGQEQWRLVPGEDYSSEFSENEGPERETFAVVGQIAVLQNLARQR
                     GTVVIDTRTGQVLTDSLPNETGADTVRVLSDGYVVRRTEDTDGKTRAWYELRDFSGEVL
                     KTVPIDNARLDALLPLEETLVHLEWGERGAAPELVVFPWEEGEPSERIASPVHVETAAL
                     ISVWRAEEATGPAAFQAVPGAVLLLEYTPGGFYDRVAGFV"
     misc_feature    complement(3584..3598)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.6"
                     /tool="antismash"
     gene            4142..4537
                     /locus_tag="PWG71_RS19315"
                     /old_locus_tag="PWG71_19315"
     CDS             4142..4537
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PWG71_RS19315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="PWG71_19315"
                     /product="hypothetical protein"
                     /protein_id="WP_274811281.1"
                     /transl_table=11
                     /translation="MYGKTAVEWEQLVDATVHLLEERARAGAVVTYTEVNRALVVRTGQ
                     RPFDFSQPDDRAAVGRLLGEAVERTLDEVGAMISAITLYQGGNDPGPGFYALAAEKGLL
                     TPKASPAQRLNFWTGQVNRVYAHYRFL"
     gene            complement(4559..5239)
                     /locus_tag="PWG71_RS19320"
                     /old_locus_tag="PWG71_19320"
     CDS             complement(4559..5239)
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013153671.1"
                     /locus_tag="PWG71_RS19320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_19320"
                     /product="SWIM zinc finger family protein"
                     /protein_id="WP_274811283.1"
                     /transl_table=11
                     /translation="MTSLTRESLRDMAGHTSFDRGIDYLDRVSGLLHQGDSVRATVSGT
                     RRYRVELRTGRRFSWDCTCPWAQEGNCCKHVVAVALVHLYEQEHGSTAPAVPDPEDYLR
                     SLDREELLSALLAETDRSPALALDLQMRAAASAADLDALRVLFDGALRLTDTVPWEQAA
                     EYARAVRGAADAVDLLASSGRVEEAMELTDLLHSLTDEAEDMVEDPDGEVVAALEHLRG
                     SGGF"
     misc_feature    5201..5211
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     gene            5275..5442
                     /locus_tag="PWG71_RS19325"
                     /old_locus_tag="PWG71_19325"
     CDS             5275..5442
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PWG71_RS19325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="PWG71_19325"
                     /product="hypothetical protein"
                     /protein_id="WP_274811285.1"
                     /transl_table=11
                     /translation="MDIETVDGASQAAPVNDAGRDTGTWDTGPVGNDTAAGFCGGLDDA
                     ATEEGAGMLF"
     gene            complement(5463..5717)
                     /locus_tag="PWG71_RS19330"
                     /old_locus_tag="PWG71_19330"
     CDS             complement(5463..5717)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PWG71_RS19330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="PWG71_19330"
                     /product="hypothetical protein"
                     /protein_id="WP_274811286.1"
                     /transl_table=11
                     /translation="MRTSPTRLPAPLNAVVCELVLMAAPERRTVPGRCTRPGCSCSGWC
                     ARFPRGRGRPEHTRRTATRRSTRSPALLHRTPTGRSRHA"
     gene            5930..6766
                     /locus_tag="PWG71_RS19335"
                     /old_locus_tag="PWG71_19335"
     CDS             5930..6766
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017570465.1"
                     /locus_tag="PWG71_RS19335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_19335"
                     /product="hypothetical protein"
                     /protein_id="WP_274811288.1"
                     /transl_table=11
                     /translation="MPLTPRAALCAALVAASTLTACAPAENLLNDVVRDVTGVPITYDE
                     IDRDDPFGGTEAEGYDEEFVVPEAAGIGEYPADRVQEAYDVTGAFLDAVYLDRTSVFEG
                     DDMRFRSLLTGQARDWYQENAGHDDPALDTRSLLFHLTPGTAEPIGDVVKVKGRMWAET
                     AQDETGWDYLAVRTEYTIVHPVARPGEKVSVRLVTSHYGEVSFYDLGDGRWEAWPNWWR
                     SAGPAHCLWDEYTFTPAYPDEMPEGERPKGWIEGDPYDLEAARDMDEQECGAIGAT"
     gene            6867..7223
                     /locus_tag="PWG71_RS19340"
                     /old_locus_tag="PWG71_19340"
     CDS             6867..7223
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017586937.1"
                     /locus_tag="PWG71_RS19340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_19340"
                     /product="cupin domain-containing protein"
                     /protein_id="WP_274811290.1"
                     /transl_table=11
                     /translation="MVGDGSPVTLIATGIDRLRERNPFKEGRVAPERLFAGEDVRIMHL
                     ALDAELTEHRAPSPIVVQVIEGTVRFAVEGEEYGLGAGGMIHVAAGVPHAVSPVDGPAR
                     ILIHLLDPARRPRE"
     gene            7433..8098
                     /locus_tag="PWG71_RS19345"
                     /old_locus_tag="PWG71_19345"
     CDS             7433..8098
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017582922.1"
                     /locus_tag="PWG71_RS19345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_19345"
                     /product="DUF429 domain-containing protein"
                     /protein_id="WP_274811292.1"
                     /transl_table=11
                     /translation="MDVVGIDGCPKGWVAVRLVDGRFDGARFCPTLAEATSMAGTVAVD
                     IPLGLVDHGWRECDGLVSALLKGHASSVFRIPPRRVVEADGYEAANALCRELTGKGLSR
                     QSHALFPRILEADALREAGVPELHEVHPELSFRVMAGRPLRWSKKTWNGQAERRRLLDS
                     AGILPPDDLAEAGSVPLVDVLDATAAAWSAHRVATGAAGSHPDPPQLDGAGGPVAIRC"
     misc_feature    complement(8286..8306)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.42"
                     /tool="antismash"
     misc_feature    8443..8454
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.96"
                     /tool="antismash"
     misc_feature    8528..8544
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.72"
                     /tool="antismash"
     gene            8559..9959
                     /locus_tag="PWG71_RS19350"
                     /old_locus_tag="PWG71_19350"
     CDS             8559..9959
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017544766.1"
                     /locus_tag="PWG71_RS19350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_19350"
                     /product="family 2B encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_274811294.1"
                     /transl_table=11
                     /translation="MSTEAPEVSTGQQKSLSTAAARNLATTTKSAPQMQGKSSRWVLKT
                     LPWVQTEGGVYRVNRRLSYTLGDGRIEFHQTGSQVKVIPRELGELALLRGFDDEEVLTD
                     LADRFTQHEIEPGQVLVSEGDPADRLYLIVHGRVSRIATGHYGASERLGVLADGDQFGE
                     EHLVEDCPTWDFTARADTAGLVLSLTKQEFAAALDRSESLSFHVELFGSLPQQSQNKYG
                     EAEIELASGHLGEFELPQTFVDYELHPREYELSVAQTVLRIHSRVADLYNKPMNQTEHQ
                     LRLTIEALRERQEHELVNNADFGLLNNADFGQRIQAQEGPPTPDDLDDLITMRRNTQYL
                     YAHPKAIAAFAKECNKRGLALDTAEVNGNRLPAWRGIPLLPCGKIPVTEHRTSSIIAVR
                     TGEDNEGVVGLHQIGLPDEVEPGLNARFMGIDDKAVISYLVSTYYSAAILVPDAVGILE
                     NIEVKPRA"
     gene            10001..11206
                     /locus_tag="PWG71_RS19355"
                     /old_locus_tag="PWG71_19355"
     CDS             10001..11206
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T1TS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052: Terpene synthase/cyclase metal-binding domain
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017544767.1"
                     /locus_tag="PWG71_RS19355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_19355"
                     /product="family 2 encapsulin nanocompartment cargo protein
                     terpene cyclase"
                     /protein_id="WP_274811295.1"
                     /sec_met_domain="T1TS (E-value: 2e-52, bitscore: 172.1,
                     seeds: 237, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSLSRMFAAPAAPETAALVDALLRDPRRPGPAELPMPGRGPAPN
                     GAGTGTPSLPVHPPGPLPSSGSVGTEVAGSRVAGGGADPVPVLYCPPAVRDDPALGDEV
                     DDRLVAWAEEVGVYPGQLDRVRAAGFGRLIMLAHPETDDPDRLLAAAKCALAEWSVDDH
                     YVDGEVEEARHDLLGQRLAIAHSVIDQAHLPRRYAPELERVVRADPVAVALRSSIENLG
                     RYATWQQVRRLRHELAIMFVAYNQEGVWYTTGQRPPVWEFLMHRHENSFVPCMALIDPV
                     AGYELPAEQFGRPEVRRVFTLAGSASVIVNDLYSMGKEDVDDFSLPRLIETEDGCSPRE
                     AMERTVAIHDELMHVFEAEAAALAATGPPELGRFLWGLWAWMGGSREWHASTARYHRTA
                     QA"
     gene            11280..12143
                     /locus_tag="PWG71_RS19360"
                     /old_locus_tag="PWG71_19360"
     CDS             11280..12143
                     /EC_number="2.1.1.255"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0042214 - terpene metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013153643.1"
                     /locus_tag="PWG71_RS19360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_19360"
                     /product="geranyl diphosphate 2-C-methyltransferase"
                     /protein_id="WP_274811296.1"
                     /transl_table=11
                     /translation="MTDTMPSTADKVLRTAYQESVAAYWNAEQDPVNIRLGDVDGLYHH
                     HYGVGDYDPSVLEGPEETRDQRVIEEMHRLETAQAELLLDHLGPLGPEDRIMDTGSGRG
                     GTSFMANARFGCSVDGVSISRKQVDFCNAQARERGVDDRVRFHFRNMLDTGLETGSLSA
                     SWNNESTMYVDLFELFAEHARLLRHGGRYVTITGCYNDVTGGRSKAVSRIDEHYTCNIH
                     PRSAYFKAMAANGLVPISVVDLTDATIPYWELRSHSSVATGVEEPFLTAYRERSFHYLL
                     IAADRI"
     gene            12225..12815
                     /gene="idi"
                     /locus_tag="PWG71_RS19365"
                     /old_locus_tag="PWG71_19365"
     CDS             12225..12815
                     /EC_number="5.3.3.2"
                     /GO_function="GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA]"
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="idi"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013153642.1"
                     /locus_tag="PWG71_RS19365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_19365"
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="WP_274811297.1"
                     /transl_table=11
                     /translation="MSHTARTAPPERAPAPADTIMLELVDEHGATTGTAEKLAAHRAPG
                     TLHRAFSVFLVDDRGRLLLQRRALDKYHSPGVWSNTCCGHPFPGERPLIAAVRRVGEEL
                     GMAPALLREAGTVTYRHPDPESGLVEHEYNHLFVGRADRPTRPDPREVAEVRAVTPGEL
                     ERLRAEAPFSVWFTTVWEAALPRLRESVPDGGW"
     gene            complement(12886..13101)
                     /locus_tag="PWG71_RS19370"
                     /old_locus_tag="PWG71_19370"
     CDS             complement(12886..13101)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PWG71_RS19370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="PWG71_19370"
                     /product="hypothetical protein"
                     /protein_id="WP_274811298.1"
                     /transl_table=11
                     /translation="MGKGSRRSGHRNLAGPAPDAEAGGPAALVRAGGLTEGAPPERLSE
                     HLDGCGDRDLLGLLARRPVGAGAGAG"
     gene            complement(13123..15396)
                     /locus_tag="PWG71_RS19375"
                     /old_locus_tag="PWG71_19375"
     CDS             complement(13123..15396)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017544786.1"
                     /locus_tag="PWG71_RS19375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_19375"
                     /product="ATP-binding domain-containing protein"
                     /protein_id="WP_274811299.1"
                     /transl_table=11
                     /translation="MPVGQSDTSGTVSPERAALAAEQGRVDAMYTRLDELRDRTAHRLA
                     AAHLQERSGFAAIVERESRAYEHARERARLGSVEEGLCFGRIDIVHDDGPEARYIGRIG
                     LRDAEYETILVDWRAPAARPFYAATPGDPQGVTRRRHLRVRRRRVVGLDDEVFDIDGLG
                     ADDRRQLVGEAALLSALRSGRTGRMGDIVATIQTEQDRVIRARLDGVLVVQGGPGTGKT
                     VAALHRAAYLLYTHRDLLERRGVLVVGPNPAFLRYIGDVLPSLGETDAVMSTLGGLFPG
                     VSAHRHDPYPVAALKGDTRVVGLLRSAVADRQRTPREAFGADDLVVEVDGTTLTVPGSI
                     CAHIVRTARGLRLGHNVARRYTVTELLSALARAEAEALGRPFQREDVRFVRERLWSEDP
                     VRDAVDALWPVLTPQALLSGLLTDRDALSRVGARAGFSPDEAALLHRPADADWTVQDVP
                     LLDEAAELLGRDDGGRRARERRAAAERAEEERYAQGVLEFTGLADEGVMDAATLAARHR
                     HDGTGLTTAERAGADREWAYGHVIVDEAQELSPMAWRTVMRRVPTRSLTVVGDLAQTGS
                     AAGAVSWTGALEPYARGKVHVERLRVNYRTPAPIMEVAADVLREAAPDEEPPESVREDG
                     APPRAVPLPADLDGLAALVDGERVRIGEGTVAVITADDRVAAVRAALPTAARAEDLEKQ
                     VVVLTATEAKGLEFDAVVVVDPEALLAAPRGANDLYVALTRATRRLIVAHIGPLPAVLG
                     RLAG"
     gene            15766..17976
                     /locus_tag="PWG71_RS19380"
                     /old_locus_tag="PWG71_19380"
     CDS             15766..17976
                     /EC_number="4.1.3.27"
                     /GO_function="GO:0004049 - anthranilate synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1018: isochorismate synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019609103.1"
                     /locus_tag="PWG71_RS19380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_19380"
                     /product="anthranilate synthase component I"
                     /protein_id="WP_274811300.1"
                     /transl_table=11
                     /translation="MTSSDAQTTTYRTRGGVTVHRQATPCDPEILTDLVRDVEYRRGGV
                     LSSGMEYPGRYDRWHLGYVDPCLEFTTRGRRVTATALNERGRVLLPVIADALETTGVDF
                     ESGPDHAAVTVPEPDPGVFFTEEERSRRPSVFTALRAVVDVLSAPEDGNLGLYGAFGYD
                     LAFQFEPIQRHIDRDPIDRDLVLHLPDAIVVRDRKRETCVRYTYDFAVPAGPGRPEART
                     RGLDRLTAPTPPVVAGEVPEDPEPGSYARVVAHAKERFRRGDLFEVVPGHRTYARCSSP
                     ARFYELLRERNPAPYEFFFNLGGGEYLVGASPEMFVRVSGDPGTGQRVETCPISGTIRR
                     GDDALGDAENIQELLSSVKEKSELTMCTDVDRNDKSRVCEPGSVRVIGRRQIEMYSRLI
                     HTVDHIEGTLRREFDALDAFLTHMWAVTVTGAPKTWAMRFIEQHETSPRRWYGGAVGVI
                     NFNGAMNTGLTLRTAHIRDGVAAVRVGATLLYDSDPEAEERETFLKARALLETLALSEE
                     DDTALARVGATKTRDDDERPGEGMKVLLVDHEDSFVNTLADYVRRHGAEVITVRYGFEP
                     SLLDRLAPDLVVLSPGPGLPVDFGMSALLDELRTRNLPVFGVCLGLQGMVEHAGGELRT
                     LDEPVHGKPGRVRALGGALLSGLGEEGLFQAARYHSTYALPELVKGYEVTAVIDAEGEE
                     PVVMAIEDRQNRRYAVQFHPESILTASVGEEIVARALRLARG"
     misc_feature    complement(18000..18015)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 19.26"
                     /tool="antismash"
     gene            18119..18259
                     /locus_tag="PWG71_RS19385"
                     /old_locus_tag="PWG71_19385"
     CDS             18119..18259
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014912075.1"
                     /locus_tag="PWG71_RS19385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_19385"
                     /product="GPGG-motif small membrane protein"
                     /protein_id="WP_274811302.1"
                     /transl_table=11
                     /translation="MGILLTILGIILLIVGVLGVVRGQLLWGIIAIVLGLFLTPGYFFG
                     I"
     gene            18459..19256
                     /gene="thyX"
                     /locus_tag="PWG71_RS19390"
                     /old_locus_tag="PWG71_19390"
     CDS             18459..19256
                     /EC_number="2.1.1.148"
                     /GO_function="GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA]"
                     /GO_process="GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="thyX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017544788.1"
                     /locus_tag="PWG71_RS19390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PWG71_19390"
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="WP_274811303.1"
                     /transl_table=11
                     /translation="MKRVEPQVRLISRPQVDYDAIADYLKEVGGQSWLERFDRGELDDH
                     LNDPQNLAEFAGRLCYRSWEPGLNPNVTRVRTDQDAYLGNILASLHGSVLEHVTFGFVL
                     HNVSRVLTHELIRHRPGVAISQESLRFVRLSDLPFWFPDWAQDDPELMERAGEMLQRME
                     EFQLWMADHFGLDDEGVKFAEKKHKTSFMRRFAPEGVATGLVWSANVRTLRHTLEARTA
                     PGAEEEIRLLFHLIGETLQKEAPALFGDYVVEDGAWVPRWRKV"
     misc_feature    19499..19514
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.23"
                     /tool="antismash"
ORIGIN
        1 cccttcatct cccccatctg ggtcaagtct ccggctgaac aggcggagga agcccttgcg
       61 ggactgaagg gccggcttga aggggaggaa gcgaaggcat accggacttt cgagtcccgg
      121 gccgaggagg tcggacagga tctgcgtgac gggccgactc cggagaccat agaacgtctc
      181 atgaacggag ggtacatcac ctggtcgtac ttcaacctgg gtggcgatgt ggagtccctc
      241 ccgatcgacc tggaccccga agaggctgcg aggaaattcg aagactacgt caacgatccc
      301 gacggttacg acggggacgt atccgaactg ctcgccctga tcaacaacat cagtatagcg
      361 gcgatggacc gacagggaaa cggtgggact ctgaagggag aagaactcga tttccttcgg
      421 gacttctacg gttccctgga agaggctggt ggcccggact cgactcctcc cggaatcttg
      481 aacttcgtgg acatcatatc ggcgaaccag gatatcgatc ccggcatggc cgaagagctg
      541 ctcggagccc tgggaaacgg acttcttgtc atctccgacg agagcctcat cggaggttac
      601 gagaaccttc ccgaaagtat cagaaataca gtggaggggc ctggcgtcga tacggacgcg
      661 atcagcccct actggtacac caacgcctcg aatctcgcgt tgatgatggg gcacaccatt
      721 ccgggcatcc agggtggtga gcagttctcc gttaacctgg cgcagacggt cggtcacgta
      781 ctggacatca ccgggccgag caatccccca tcgatggggt ccacgtactt cgagagactt
      841 gtggaggtcg cctcgcgcaa tgaggacgcc atgcacgcga ttctcaccgg ccaaggacag
      901 tacgagcacc cggaccacgg ccttgacccg gagatgaccc tgcgcggtat ctacacctat
      961 gactgggggg acgagggcga gagcgcgggc aggctgaccg actggatcgc cgagcaggct
     1021 cacggagacg atgccgaggc ggagcgggcg ggcgaggcga tggccgctct catgcagctg
     1081 ttcggagacc ctgccttcag cgacgccctc gccgggacgg gttactccgt ggccggcgag
     1141 gtcgtgggtc ctgacggcag cgttgtcgag accgaatggg caaacgcctc cgcaggtcac
     1201 gtgaatccac agttggcctg ggcatggtcc gagctcttca gtgcctacat cgacgacttc
     1261 ggttcgggct acgggactcc cagcgggacg gaaggtattc cggaggacca acgcgaatct
     1321 cagtggacca ggagcgacgg tttgcagctc acccccggcg accgtgagga cttcgttcgc
     1381 ctcatcatgg gcgatgaaga cgccgcttct cggatctacg ccgaaaccct ggcgttcgag
     1441 gaggagaagc tctacgagtt cttcgcgaac caggaccata tcgatgatgc gccggcggaa
     1501 acagcgggta cccttcgcgg cctggtcgac tcagcgcttt tcgaggaagc cgagatcaga
     1561 ttcgacaaga gttctgagga cgccaactac aggaacaagg tcaccggcta cgccgcggac
     1621 atcgtcggtg ccgcactggc ggaggccccc tacaggggaa gcggtctcgt ggccgaggtc
     1681 gtcaagatcg gcatcaagga gggtttcgac gcgagctctt atcccgccga actccaggtc
     1741 gatgacgcct ggaaggggga gtgggagatc aacgagacct tcaagatcgc ggctatgcgg
     1801 gaactggccg cgcaggatcc ggacctgctc gaaaggctca ggtatctcga ggggaccaat
     1861 ggatcccctg tggtggacac ggacgcgggg tctcatattc ctcctcgagc cagtgactgg
     1921 gatctggaag gtgacgagtc caggaccgtg atcaggcaag catggcgaga aatcgagtcg
     1981 gatctgtggg gtgccaccgg gtacgacctc gactatgcgc tggaagagta cgtgaaagcc
     2041 tacaacactg agcgtggaag aatggacgag aactatgatg cctccggttc ggaagactga
     2101 tgcgtgaacg ctttttttcg tccctgctcg tggaaacgtg gtccgtgacg accagtagga
     2161 ccgttacggt catcgccgtg gccttcgcca ccgtggtgag cggatgttcg tccatgcccg
     2221 gaggtgaacc aggggaagcc cgttcgggtt cggaggggct tcacgaggtc gccgagcatc
     2281 ctctggagga gcgtgccgtt ccgggcagca tctccgagtc ggcatggacg tgggaaggcc
     2341 cccgggcagt gggggtggag gaggtcctcc ccgttcccac aggtgccgtc ctgcgcctga
     2401 ccgatggcgt ggtggccctg gacccggtga cgggcgaaga agtgtggacg tttcgtcttc
     2461 cgggcgcgga ggccgacgcc gcgacctccc ctgacggggc cgccgtagcg gtgatcgcac
     2521 aggggaccct ggtgctgttg aactcgtcca cgggcgagga actggacacc agggagtacg
     2581 acacggacgg cggcagtgga ttggagttgt caggtgtggg attggtggcc gacaccggca
     2641 tggtcgcggc caccgaggac cccggttctg gcctggaagt gcgcctgcgt ccttgggccg
     2701 agcaggacga ggggtggacg gcgggccccc tcctgtgtgc ggacggttcg gcgggtaccg
     2761 gcatcgagga cggattcctc accccgacgg gtgtggtcgt ggtcttcggt tgttccggcg
     2821 gcacctcggc catggtgagc ctggacgcct cttcagggca ggagcagtgg cgtctggttc
     2881 cgggggagga ctacagcagc gagttcagcg agaacgaggg cccggagagg gagaccttcg
     2941 ccgtggtggg acagatcgcg gttttgcaga acctggcccg tcagcgaggc accgttgtga
     3001 tcgacacccg aaccggtcag gtgctgacgg actccttgcc gaacgagacg ggagccgaca
     3061 ccgtgcgggt gctctccgac ggctatgtgg tcaggcgcac cgaggacacc gatgggaaga
     3121 cacgggcctg gtacgaactg cgggacttct ccggcgaggt gctgaagacg gtgccgatcg
     3181 acaatgccag gctcgacgcg ctgctgccgt tggaggagac gcttgttcac ctggagtggg
     3241 gcgaacgcgg ggccgcgccg gaactggtgg tcttcccgtg ggaagaaggg gaaccctcgg
     3301 agaggatcgc ttcaccggtg cacgtggaaa cagcggccct catcagtgtc tggcgtgcgg
     3361 aggaggccac cggcccggcg gccttccagg ccgttcccgg cgcggtattg ctcttggagt
     3421 acacccccgg cggtttctac gaccgtgtgg ccgggttcgt ctgagccggc gttccgtcgg
     3481 gccgccgggt ggtggccgga tacggcgcag ggatgccgtg ccggtggggg ttctgggatc
     3541 atcggggcga ccgaggagcg gatcccctac agcgacggcg acccggaccg gatcggcccc
     3601 taccggctgg agggggctgg gcggcgggaa cgccggcggc aggtggcagg aacaggaagg
     3661 ggccaccgag gtccttccca ggggaggggc gctgcggccg acggcggtcg tgacggaggc
     3721 cgagggcgag tcggaccgga ttcctgggtc tggaacggac ccggaaccgg acggtcggac
     3781 cgccaggagg gcggtgggcc tggccagggc cgatcttctc gggattctgg ccatcgttct
     3841 gccggccgcc ttgagtcttt ccatccccgg cgcaccggct tgagcgatcg tggcgaacct
     3901 ggtggtgggc ctcatcgggg caccgctgcg cttcctggga gggccgtggg ccgccgtgct
     3961 gatcgccgtc atgttcggtc tggtcagcgc cggggcgcgg tgaccgccgt acgtgcgcgg
     4021 cggtccgtcg ctttccggac ctaagactct ggagcggctc gcggggctcg gatacattcg
     4081 cgccaccgtg cggccccccg gccgccggtt cacggcagtg caccgacgcg acggaggaag
     4141 cgtgtacgga aagaccgctg tcgagtggga gcagttggtc gacgcgaccg tgcacttgct
     4201 ggaggagcgt gcgcgggcgg gtgcggtcgt gacctacacc gaggtgaacc gcgcgctcgt
     4261 ggtgcgtacc ggacagcgcc ccttcgattt ctcccagccg gacgatcggg ccgctgtggg
     4321 ccgcctgctc ggggaggcgg tggagcgcac ccttgacgaa gtcggtgcca tgatctcggc
     4381 gatcaccctc taccagggcg ggaacgaccc cggccccggc ttctacgcct tggcggcgga
     4441 gaaggggctg ctgaccccca aggcctcccc ggcccagagg ctgaacttct ggaccgggca
     4501 ggtgaacagg gtctacgccc actaccggtt cctctgaggc cggtcgcggc gggcggggtc
     4561 agaatccgcc ggatccgcgg aggtgttcca gagcggcgac gacctcgccg tcggggtctt
     4621 cgaccatgtc ctcggcttcg tcggtgaggg agtgcagcag gtcggtcagc tccatcgcct
     4681 cctcgacgcg cccggacgag gccaggaggt cgacggcgtc ggccgcgccg cgcacggcgc
     4741 gggcgtactc ggcggcctgc tcccagggga ccgtgtcggt gaggcgcagg gccccgtcga
     4801 agaggacgcg cagggcgtcg aggtctgcgg cggaggcggc ggcccgcatc tggaggtcca
     4861 gggcgagtgc gggggagcgg tcggtctcgg ccaggagggc agagaggagc tcctcccggt
     4921 ccagggagcg caggtagtcc tcggggtcgg gtacggcggg cgccgtggac ccgtgctcct
     4981 gctcgtacag gtgcaccagg gccaccgcga cgacgtgctt gcagcagttg ccctcctgcg
     5041 cccacgggca ggtgcagtcc caggagaacc gccgtccggt gcgcagttcc acgcggtagc
     5101 ggcgggtgcc cgacacggtg gcccgtacgc tgtcgccctg gtggaggagg ccggacaccc
     5161 ggtccaggta gtcgatgccg cggtcgaagg acgtgtgtcc ggccatgtca cggagggatt
     5221 cacgggtgag ggaggtcatg gctccatgac accacggtgg gtgcggttcc cgggatggat
     5281 atcgaaacgg tcgacggcgc ttcgcaggct gcccctgtga acgatgcggg gagggacacg
     5341 ggcacctggg acaccggccc ggtcggtaat gacacggcag cgggcttctg cggtggcctc
     5401 gacgacgcgg ccaccgagga aggcgcgggc atgttgttct gagttcggcg ccgaggtcgc
     5461 ggtcaggcgt ggcgggagcg gccggtgggg gtgcggtgga gcagggccgg ggagcgcgtg
     5521 ctcctgcggg tggcggttct gcgggtgtgt tcggggcgtc ctcggcccct ggggaaccgg
     5581 gcgcaccagc ccgaacacga gcacccgggc cgagtgcagc ggcccgggac ggtgcggcgc
     5641 tccggggccg ccatcagcac cagctcgcac accaccgcgt tgagcggggc gggcaggcgg
     5701 gtcgggctcg tgcgcatgcg ctccctcagc tcctcggctc cggaggggtt ccggtggtcc
     5761 gtctccgggc gaacctaccc ctgggcccgg aatcggggaa aggggttttc ccgcgctcgc
     5821 ggcacatagg gacgaagcgg ggaccggggc gccgacctgt cggaggaatg gggtaaaact
     5881 gtgcgaagtt cgcatatccc ctgatggaca agtgaagacg ggatccccca tgcccctgac
     5941 cccgcgtgcg gcgctgtgcg cggcgttggt cgccgcgtcg accctcaccg cctgtgcccc
     6001 cgccgagaac ctgctgaacg atgtcgtccg cgacgtcacc ggcgtcccga tcacctatga
     6061 cgagatcgac cgcgacgacc cgttcggcgg taccgaggcc gaggggtacg acgaggagtt
     6121 cgtcgtgccc gaggcggccg ggatcgggga gtaccccgcc gaccgggtcc aagaggccta
     6181 cgacgtcacc ggcgcgttcc tggacgccgt ctacctggat cggacctccg tcttcgaggg
     6241 cgacgacatg cgcttccggt ccctgctcac cggacaggcc cgggactggt accaggagaa
     6301 cgccggacac gatgaccccg ccctcgacac ccgctccctg ctcttccacc tgacgcccgg
     6361 caccgccgaa cccatcggtg acgtggtcaa ggtgaagggc cggatgtggg ccgagacggc
     6421 ccaggacgag accgggtggg actacctggc ggtgcgcacc gagtacacga tcgtgcaccc
     6481 ggtcgcccga ccgggggaga aggtgtcggt gcggctggtc acctcgcact acggcgaggt
     6541 gtccttctac gacctcggtg acggccgctg ggaggcctgg ccgaactggt ggcgctcggc
     6601 gggcccggcc cactgcctgt gggacgagta caccttcacg cccgcctacc ccgacgagat
     6661 gcccgagggc gaacgcccca aggggtggat cgagggggat ccctacgacc tggaggccgc
     6721 ccgcgacatg gacgagcagg agtgcggcgc catcggcgcc acctgaccca ggctccgacg
     6781 agggcccgtc cggcgacggg cgggccttcc cgctttccga ggccggtgtg gaaccctgga
     6841 ggcatacgag cgggcggagg aacgacatgg tcggggacgg ttcaccggtg accctcatcg
     6901 ccaccgggat cgatcggttg cgcgagcgca atcccttcaa ggaggggcgc gtcgcgcccg
     6961 agcggctgtt cgccggcgag gacgtgcgga tcatgcacct cgccttggac gccgagttga
     7021 ccgagcaccg agccccctcg ccgatcgtcg tgcaggtcat cgagggaacg gtgcgtttcg
     7081 cggtggaggg ggaggagtac gggttgggtg cgggcggcat gatccacgtc gcagccggcg
     7141 tcccgcacgc cgtctccccc gtcgacggcc ccgcgcggat cctcatccac ctgctcgacc
     7201 ccgcgcgacg tccccgggag tgagcgcttc gggatcggcg gggcgcatcc agggcacctg
     7261 acgcttcggg gggcgtcggc cgacgggccg gcgccccgtg ccctcgccgg gcgctcgcgc
     7321 ccgccccggc gggcggtgct tccgggcccg tccgattctt ccgcgttccc gccctcggat
     7381 ccgccgggcg tggctgacgc gctgtcgcga tccgggcgtc cacactggtg gcatggacgt
     7441 ggtggggatc gatgggtgtc cgaagggctg ggtggcggtc cgtctcgtgg acgggcgttt
     7501 cgacggggcc cggttctgtc cgaccctggc cgaagcgacc tccatggccg ggaccgtcgc
     7561 ggtcgacatc cccttgggcc tggtcgatca cgggtggcgc gagtgcgacg ggctggtgtc
     7621 ggcgcttctg aaggggcacg cctccagtgt cttccggata ccgccgagac gggtcgtcga
     7681 ggccgacggc tacgaggccg ccaacgccct gtgcagggag ctgacgggga agggcctcag
     7741 tcgccagtcc cacgccctgt tcccgaggat cctggaggcc gacgccctgc gcgaagccgg
     7801 tgtgccggag ctgcacgagg tccaccccga gctctcgttc cgggtgatgg cgggccgccc
     7861 cctccggtgg tccaagaaga cctggaacgg ccaggcggaa cgccgccgcc tgctcgactc
     7921 cgccgggatc ctgccccccg acgacctcgc ggaggcgggt tcggtccccc tcgtcgacgt
     7981 cctcgacgcg accgcggcgg cgtggagcgc ccaccgcgtc gccacggggg cggccgggtc
     8041 ccaccccgat cccccgcagc tcgacggggc ggggggaccg gtcgcgatcc gctgctgaga
     8101 ccccgcgcga ccggatggcc ggaacccgca ctccaacggg accgagaccc ggtgcggcgg
     8161 ccgcgccctc agagcgattc cgccgctttt cgtgtgcctc attccttatg tcgttctgaa
     8221 ggggccgatg gtccggggat gaaatttcat gatctttcgg gcaggcgtgt gatatgggtc
     8281 gcggaaatgc atccgaaggt ttatttttaa gggtcttagt atttttgtgt tatgggatgg
     8341 tggatcatgg tgattggccg gtgaattcgg ctttctcctt gcggtcacca cttctgtgcc
     8401 cctagtgtga gcagcgcttt cgtggtcggc gtcgtgtgct cggaaatcgc ttccgaatcg
     8461 cgatggatgt ccgattcgcg atttcccctc cctcttccgg gtccctttcg ggtgcgcgga
     8521 acgggttctg cacacgacac acaggagatg tacggccgat gtcgaccgaa gcccccgagg
     8581 tgagtaccgg tcagcagaag agcctgagca ccgcggccgc gcggaatctg gcgaccacca
     8641 ccaaatccgc cccccagatg cagggcaaga gttcccgctg ggtcctcaag accctgccct
     8701 gggtgcagac cgagggcggt gtctaccggg tcaaccgtcg gttgtcctac acactcggcg
     8761 acggccgcat cgagttccac cagacgggat cgcaggtcaa ggtcatcccc cgtgaactcg
     8821 gcgagctggc gctgctgcgc ggcttcgacg acgaggaggt gctcaccgac ctcgccgacc
     8881 gcttcaccca gcacgagatc gagcccggcc aggtcctggt ctccgagggc gacccggccg
     8941 accgcctgta cctgatcgtg cacggccggg tgagccggat cgcgaccggc cactacgggg
     9001 ccagcgaacg gctgggcgtg ctcgccgacg gtgaccagtt cggtgaggag cacctggtcg
     9061 aggactgccc gacctgggac ttcaccgccc gggccgacac cgccggtctg gtgctctcgc
     9121 tgaccaagca ggagttcgcg gccgcactgg accgctccga gtcgctgagc ttccacgtgg
     9181 agctgttcgg atccctgccg cagcagagcc agaacaagta cggcgaggcc gagatcgagc
     9241 tggcctcggg ccacctcggc gagttcgagc tgccgcagac cttcgtcgac tacgagctcc
     9301 acccccgcga gtacgagctc agcgtcgccc agaccgtgct gcgcatccac agccgggtcg
     9361 ccgacctcta caacaagccg atgaaccaga ccgagcacca gctgcggctg accatcgagg
     9421 ccctgcgcga gcgccaggag cacgagctgg tcaacaacgc ggacttcggt ctgctcaaca
     9481 acgccgactt cggccagcgc atccaggccc aggagggtcc gcccaccccg gacgacctgg
     9541 acgacctcat cacgatgcgc cgcaacaccc agtacctgta cgcccacccc aaggcgatcg
     9601 cggcgttcgc caaggagtgc aacaagcgcg gtctggccct ggacaccgcc gaggtgaacg
     9661 gcaaccggct gcccgcctgg cggggcatcc cgctgctgcc ctgcggcaag atccccgtca
     9721 ccgagcaccg gacctcctcg atcatcgcgg tgcgcaccgg cgaggacaac gagggcgtcg
     9781 tcggcctcca ccagatcggg ctgcccgacg aggtggagcc gggcctcaac gcccggttca
     9841 tgggcatcga cgacaaggcg gtgatctcct acctggtcag cacctactac tcggccgcga
     9901 tcctggtgcc cgacgcggtc gggatcctgg agaacatcga ggtcaagccg cgtgcctgac
     9961 gcatcgcccg agacgacacc gacgaaggag gggtgagacg gtgtcatcgc tgtccaggat
    10021 gttcgcggcg cccgccgccc ccgagacggc ggcgctggtc gacgcgctgc tgcgcgaccc
    10081 cagacgtccg ggccccgccg agctgccgat gcccggccgg ggccccgcgc cgaacggcgc
    10141 ggggaccggg accccgtccc tgccggtcca cccgccgggc ccgctcccgt cctcgggatc
    10201 ggtggggacc gaggtggccg ggtcacgggt cgcgggcggg ggagccgacc ccgtgccggt
    10261 cctgtactgc cctccggcgg tgcgcgacga ccccgcgctc ggcgacgagg tggacgaccg
    10321 gctcgtcgcc tgggccgagg aggtcggcgt ctacccgggt cagctcgacc gggtgcgggc
    10381 cgccgggttc gggcggctca tcatgctcgc ccaccccgag accgacgacc ccgaccggct
    10441 gctggcggcc gccaagtgcg ccctcgcgga gtggtcggtg gacgaccact acgtcgacgg
    10501 ggaggtcgag gaggcccggc acgacctgct cgggcagcgc ctggccatcg cccactcggt
    10561 gatcgaccag gcgcacctgc cgcgccgcta cgcccccgaa ctggaacggg tcgtgcgggc
    10621 cgaccccgtc gcggtggccc tgcgctccag tatcgagaac ctgggcaggt acgccacctg
    10681 gcagcaggtg cgcaggctcc ggcacgaact ggccatcatg ttcgtcgcct acaaccagga
    10741 aggcgtctgg tacaccaccg ggcagcgccc gccggtctgg gagttcctca tgcaccggca
    10801 cgagaacagc ttcgtgccgt gcatggccct catcgacccg gtggccggat acgagctgcc
    10861 cgccgagcag ttcggtcggc ccgaggtccg gcgggtgttc accctggccg ggtccgccag
    10921 cgtcatcgtc aacgacctct actccatggg caaggaggac gtcgacgact tcagcctgcc
    10981 ccgcctcatc gagaccgagg acggctgctc gccccgcgag gcgatggagc gcaccgtcgc
    11041 gatccacgac gagctcatgc acgttttcga ggccgaggcc gccgcactgg ccgccaccgg
    11101 gcccccggaa ctgggccgat tcctctgggg cctgtgggcc tggatgggcg gcagccggga
    11161 gtggcacgcc agcaccgccc gctatcaccg gaccgctcag gcctgaaccg agcgcacgct
    11221 ccacgggacc ggcccccggg tcggcccgcg cacaccatga cgacaaggag ttcctctcga
    11281 tgaccgacac catgccctcg accgcggaca aggtcctgcg caccgcctac caggaatcgg
    11341 tggccgccta ctggaacgcc gaacaggacc cggtcaacat ccgcctcggc gacgtggacg
    11401 gcctgtacca ccaccactac ggggtggggg actacgaccc ctccgtcctg gagggccccg
    11461 aggagacccg ggaccagcgc gtcatcgagg agatgcaccg cctggagacg gcccaggccg
    11521 agctgctgct cgatcacctc gggccgctgg ggcccgagga ccggatcatg gacaccggat
    11581 ccgggcgggg cgggacgagc ttcatggcca acgcccggtt cggctgctcc gtggacgggg
    11641 tgagcatctc ccgcaagcag gtggacttct gcaacgccca ggcccgcgag cgcggggtgg
    11701 acgaccgggt gcgcttccac ttccgcaaca tgctcgacac cgggctggag accgggtcgc
    11761 tcagcgcgag ctggaacaac gagtccacga tgtacgtgga cctgttcgag ctcttcgccg
    11821 agcacgcccg cctgctccgc cacggcggcc gctacgtgac gatcaccggc tgctacaacg
    11881 acgtgaccgg cggccgctcc aaggcggtca gccgcatcga cgagcactac acgtgcaaca
    11941 tccatccgcg cagcgcctac ttcaaggcga tggcggccaa cgggctggtc cccatctccg
    12001 tggtggacct gaccgacgcc accatcccct actgggagct gcggtcccac tcctccgtcg
    12061 ccaccggcgt cgaggagccc ttcctgaccg cctaccgtga gcgcagcttc cactacctgc
    12121 tcatcgcggc cgaccgcatc tgagcacccg gggccgtgcc cgtccgcggg cacggccccg
    12181 acgccgcaca cggcagtcac cacccgtacg cacgaaggac ggcgatgtcc cacaccgcga
    12241 gaaccgcacc gcccgagcgc gcgccggcgc ccgccgacac gatcatgctc gaactggtcg
    12301 acgagcacgg ggccaccacg ggcaccgccg agaaactggc ggcgcaccgg gcgccgggca
    12361 ccctgcaccg ggccttctcg gtcttcctcg tcgacgaccg cggccgactg ctcctccagc
    12421 gccgggccct ggacaagtac cactcgcccg gtgtgtggtc caacacctgc tgtggccacc
    12481 cctttcccgg ggaacggccg ctcatcgccg ccgttcgacg ggtcggcgag gaactcggga
    12541 tggcgcccgc cctgctccgg gaggccggca cggtgaccta ccgccacccc gacccggagt
    12601 cggggctggt ggagcacgag tacaaccacc tcttcgtcgg ccgcgccgac cggccgacgc
    12661 gccccgaccc ccgggaggtg gcggaggtcc gcgcggtcac ccccggagaa ctggaacggc
    12721 tgcgagccga ggcaccgttc tcggtgtggt tcaccacggt gtgggaggcc gctctccccc
    12781 ggctccggga gtcggtgccc gacgggggct ggtgaccgct tcccggcggg cccaccgaac
    12841 cttccagggc ccaccgaacg ctccggggcc gaccggtccg ggctctcacc ccgcgccggc
    12901 gccggcgccc accggccggc gggccaggag gccgaggaga tcccggtccc cgcagccgtc
    12961 caggtgttcc gacaaccgtt ccggtggggc gccctcggtg aggccgccgg cccggaccag
    13021 ggcggccgga ccgccggcct ccgcgtccgg ggccggaccg gcgaggttgc ggtgcccgga
    13081 ccggcggctt cccttcccca cgccgctcac cgggccgtcg cctcagccgg ccagcctccc
    13141 caggacggcg gggagcggtc cgatgtgggc gacgatgagg cggcgggtgg cgcgggtcag
    13201 ggcgacgtac aggtcgttgg caccgcgcgg cgccgccagc agggcctccg ggtccacgac
    13261 caccaccgcg tcgaactcca gccccttggc ctcggtggcg gtcagcacca ccacctgctt
    13321 ctccaggtcc tcggcgcggg cggccgtcgg cagggccgcc cgtaccgccg ccacccggtc
    13381 gtcggcggtg atcaccgcga ccgtgccctc gccgatccgg acccgttccc cgtccaccag
    13441 cgcggccagc ccgtccaggt cggcgggcag gggcaccgcg cgcggcgggg cgccgtcctc
    13501 gcgcaccgac tccgggggct cctcgtccgg ggcggcctcg cgcagcacgt cggcggcgac
    13561 ctccatgatc ggcgcgggcg tgcggtagtt cacccggagc cgttccacgt gcaccttgcc
    13621 gcgcgcgtac ggttccagcg ccccggtcca ggagaccgcc ccggcggcgc tgccggtctg
    13681 ggcgaggtcg ccgacgaccg tcagggagcg ggtgggcacc cggcgcatca ccgtgcgcca
    13741 ggccatgggg gagagctcct gggcctcgtc gacgatgacg tgcccgtagg cccactcccg
    13801 gtccgcgccg gcgcgttcgg cggtggtgag gccggtgccg tcgtggcggt ggcgggcggc
    13861 cagggtcgcg gcgtccatca ccccctcgtc ggccaggccg gtgaactcca ggaccccctg
    13921 ggcgtagcgc tcctcctcgg cgcgctcggc ggcggcacgg cgctcgcggg cgcggcgccc
    13981 gccgtcgtcg cggccgagca gttcggccgc ctcgtcgagc agcggcacgt cctggaccgt
    14041 ccagtcggcg tcggcgggac ggtggagcag ggcggcctcg tcgggggaga acccggcccg
    14101 ggcgccgacc cgggacagcg cgtcgcggtc ggtgaggaga ccgctcagca gggcctgcgg
    14161 ggtcagcacc ggccacaggg cgtccacggc gtcgcgcacc gggtcctcgg accacaggcg
    14221 ttcgcggacg aaccgcacgt cctcccgttg gaacgggcgg cccagggcct cggcctcggc
    14281 tcgggcgagc gcggagagga gttcggtgac ggtgtacctg cgggcgacgt tgtgccccag
    14341 gcgcagccct cgcgcggtgc gcacgatgtg cgcgcagatc gatccgggca cggtgagggt
    14401 ggtgccgtcg acctccacca cgaggtcgtc ggcgccgaac gcctcgcgcg gggtgcgctg
    14461 tcggtcggcg acggcggacc ggagcagacc gacgacgcgg gtgtccccct tgagcgccgc
    14521 caccgggtac gggtcgtgcc ggtgggcgga caccccgggg aagagtccgc cgagggtgct
    14581 catgaccgcg tcggtctccc cgagggaggg caggacgtcg ccgatgtagc gcaggaacgc
    14641 cgggttgggt cccaccacca ggacgccgcg ccgctccagt aggtcgcggt gggtgtagag
    14701 caggtacgcg gcgcggtgca gggcggcgac ggtcttgccg gtgccgggcc cgccctggac
    14761 cacgagcacg ccgtcgagcc gggcccggat cacccggtcc tgttcggtct ggatggtggc
    14821 gacgatgtcg cccatgcggc cggtacggcc gctgcgcagg gccgagagca gggcggcctc
    14881 accgacgagc tggcggcggt cgtcggcgcc gagcccgtcg atgtcgaaca cctcgtcgtc
    14941 caggccgacg acccgtcggc ggcgcacgcg caggtggcgt cggcgggtca ccccctgcgg
    15001 gtcgccgggg gtggcggcgt agaaggggcg ggcggccggg gcccgccagt ccacgaggat
    15061 ggtctcgtac tcggcgtcgc gcaggccgat ccgaccgatg tagcgggcct cggggccgtc
    15121 gtcgtggacg atgtcgatcc ggccgaagca cagcccctcc tccaccgaac cgaggcgggc
    15181 gcgctcccgc gcgtgttcgt acgcgcggga ctcgcgttcc acgatggcgg cgaaaccgga
    15241 gcgttcctgg aggtgggcgg cggcgagccg gtgcgcggtg cggtcgcgca gttcgtccag
    15301 ccgcgtgtac atcgcgtcga cccgcccctg ttcggcggcc agtgcggcgc gctcggggga
    15361 gaccgttccg gaggtgtcgg attgacccac gggcatcctt ctgataggct aagcgctaag
    15421 gacaagattt ttcttggtcc ttggcgtgca gagtttccca ccgtagcacg ccgtggcccc
    15481 cggcatggac ggggcgggtg agagcgcccc gctcggccgt tcccctcccg gcgacgccga
    15541 cacggatcac gcggactttc cccgaaggct cctcaggcgc ccccgcaccc gctacactct
    15601 gccccatgcg aaacacctcc gttcaggccc agtggtggcg ccgcctctag gcggcgtcga
    15661 tgtttcgcgt atctctcgca gcgaccgccc cggaccggcg gtcgtttccg tttcacggcc
    15721 cccggccggt gtgacccacc ccgaccagcg ccagaaggac cggccgtgac atccagcgac
    15781 gcacagacca ccacgtaccg cacccggggc ggcgtgaccg tccaccgcca ggccaccccc
    15841 tgcgatccgg agatcctcac cgacctcgtc cgtgacgtcg agtaccgtcg cggcggtgtc
    15901 ctgtcctccg gtatggagta tccgggccgc tacgaccgct ggcacctggg gtacgtcgac
    15961 ccgtgcctgg agttcaccac ccgcggccgt cgcgtcaccg ccaccgccct caacgaacgc
    16021 ggccgcgtgc tgctcccggt catcgccgac gcgctggaga ccaccggtgt cgacttcgag
    16081 agcggacccg accacgcggc cgtcaccgtc cccgaacccg accccggagt cttcttcacc
    16141 gaggaggagc gcagccgccg tcccagcgtc ttcaccgccc tgcgcgccgt cgtcgacgtc
    16201 ctgagcgccc ccgaggacgg caacctgggc ctgtacggcg ccttcggcta cgacctggcc
    16261 ttccagttcg aacccatcca gcggcacatc gaccgcgacc ccatcgaccg cgacctggtc
    16321 ctgcacctgc ccgacgcgat cgtcgtgcgc gaccgcaagc gcgagacgtg cgtgcgctac
    16381 acctacgact tcgccgtccc ggccggcccc ggccggcccg aggcccggac ccggggactg
    16441 gaccggctga ccgcgcccac cccgccggtc gtcgccgggg aggtccccga ggaccccgag
    16501 cccggttcct acgcccgcgt cgtcgcccac gccaaggaga ggttccggcg cggcgacctg
    16561 ttcgaggtgg tccccggaca ccgcacctac gcccgctgct cctcgccggc ccgcttctac
    16621 gagctgctgc gcgagcgcaa ccccgccccc tacgagttct tcttcaacct gggcgggggc
    16681 gagtacctgg tcggcgcctc cccggagatg ttcgtgcggg tcagcggtga ccccggaacc
    16741 ggccagcggg tggagacgtg ccccatctcc ggcaccatcc gccggggcga cgacgccctg
    16801 ggcgacgcgg agaacatcca ggagctgctc tcctccgtca aggagaagtc cgagctcacc
    16861 atgtgcaccg acgtcgaccg caacgacaag tcgcgggtgt gcgaacccgg aagcgtccgg
    16921 gtcatcggtc gccgccagat cgagatgtac tcccggctca tccacaccgt cgaccacatc
    16981 gagggcaccc tgcgccggga gttcgacgcc ctggacgcct tcctcaccca catgtgggcg
    17041 gtgaccgtca ccggcgcccc caagacgtgg gcgatgcgct tcatcgagca gcacgagacc
    17101 agcccccgcc gctggtacgg cggcgcggtc ggggtgatca acttcaacgg cgccatgaac
    17161 accgggctga cgctgcgcac cgcccacatc cgcgacggcg tcgcggcggt gcgggtgggc
    17221 gccaccctgc tctacgactc cgaccccgag gccgaggagc gcgagacgtt cctcaaggcg
    17281 cgggccctgc tggagaccct cgccctgagc gaggaggacg acaccgccct cgcgcgggtg
    17341 ggcgccacga agacgcggga cgacgacgag cggccggggg agggcatgaa ggtcctcctg
    17401 gtcgaccacg aggactcctt cgtcaacacc ctggccgact acgtgcgacg ccacggggcc
    17461 gaggtgatca cggtccgcta cgggttcgag ccctcgctcc tggaccgact ggccccggac
    17521 ctggtggtgc tctcgcccgg cccgggtctg cccgtcgact tcggcatgtc ggccctgctc
    17581 gacgaactcc ggacccggaa cctgccggtg ttcggggtgt gcctgggatt gcagggcatg
    17641 gtggagcacg ccggcgggga gctgcgcacc ctggacgagc ccgtgcacgg caagccgggg
    17701 cgggtccgcg ccctgggcgg cgcgctgctc tccggactcg gggaggaggg tctgttccag
    17761 gcggcccgtt accactccac ctacgccctg cccgaactgg tgaagggcta cgaggtcacc
    17821 gccgtcatcg acgcggaggg cgaggagccg gtggtgatgg ccatcgagga ccggcagaac
    17881 cgccggtacg cggtccagtt ccatccggaa tcgattctca cggcctccgt cggggaggag
    17941 atcgtcgcac gggcgttgcg cctggcccga ggctgacggg gaaggggggc gcgggcgagt
    18001 gtgacggggg tcacattcgc ccccgctcag ctgcgaaaaa tgttgtctgt cgacaacatt
    18061 ttaagcttct tggtgcttcg tcttgtttga tccgcttctt ctcggtcaga ggtggggcat
    18121 gggtattctg cttacgatcc tcggaatcat tcttctcatc gtcggcgtcc tgggcgtggt
    18181 gcgcggacag ctcctctggg gcatcatcgc catcgtgctc ggactcttcc tcacccccgg
    18241 ctacttcttc ggtatctagc cggatgaccc cacgaccccg gcgcggtcct cgaccgcgcc
    18301 ggggcggccc catgccttcg gtccgcgtgg gacccggccg gacgtgtacc ctcggggaag
    18361 gctgatcagc cccaccccgt tccacgtccc ccgtgcccgg ttccggtcac ggcccagcgg
    18421 cgtgcccggg gcggggccac gacacgggag gcagtgcagt gaagagggtc gaaccgcagg
    18481 tccgactgat ctcgcgtccg caggtggact acgacgcgat cgccgactat ctcaaggagg
    18541 tcggcgggca gtcctggctg gaacgcttcg accgcggcga gctcgacgac cacctcaacg
    18601 acccgcagaa cctcgccgag ttcgccgggc gcctgtgcta ccgctcctgg gagccgggcc
    18661 tgaacccgaa cgtcacccgc gtgcgcaccg accaggacgc ctacctgggc aacatcctgg
    18721 ccagcctgca cggctcggtg ctggagcacg tcaccttcgg gttcgtcctg cacaacgtgt
    18781 cccgcgtcct gacccacgaa ctcatccggc accggccggg cgtggccatc tcccaggagt
    18841 cgctgcgctt cgtgcgcctg agcgacctgc cgttctggtt ccccgactgg gcccaggacg
    18901 acccggagct gatggagcgc gcgggcgaga tgctccagcg gatggaggag ttccagctct
    18961 ggatggccga ccacttcggc ctggacgacg agggcgtcaa gttcgcggag aagaagcaca
    19021 agacctcctt catgcgccgt ttcgcccccg agggcgtggc caccgggctg gtgtggagcg
    19081 ccaacgtgcg caccctgcgt cacacactgg aggcccgcac cgcccccgga gcggaggagg
    19141 agatccgcct gctcttccac ctcatcggcg agaccctcca gaaggaggcc ccggccctgt
    19201 tcggcgacta cgtcgtcgag gacggcgcct gggtcccgcg gtggcgcaag gtctgaccgc
    19261 accctgatca ccgagcgggg ctcgaccgga taccggtcgg gccccgcttc cacgtgttca
    19321 gtaccccaga tccgccaggc gttccgacag ccggtcggcc cgcggatcgt cgtggtcgcg
    19381 caggtactcc agagccgctc gccacacctc agcggtgcga gtgggttcgc ccatcaccag
    19441 gtgggcttcg cccaggccga tgaaggccac cgcccggctg tcccgatcgt cacgatcctc
    19501 gaactcccgt gcggccgtcc ggtactccgc caccgcctcc tctgcccggc cggtctcggt
    19561 gaggatgcga cccagggtgc accgtgccca ggccgtactc caggcggccc cgatctcctc
    19621 gaaggccgtg acggccgaac gacagtgccc gaaggcctcg tcccaccgcc ccagatcagc
    19681 gttgatctcg gcgaggctga gcagccccat ggccgcgccc cgatcctctc ccgcctcccg
    19741 gaagaccgtg acggcctgct cgatgtactg gaccgcccta ccgtctcccc gttccccgag
    19801 ctgccatagg accgttcccg actgtcgcag ggagaacccc tccacccacg ggtcccccgc
    19861 ctttcggccg gccgacaacg ccgcaccgta gtgacggacg gccgcgtcga ggttccccga
    19921 caaccactgg gcgtcaccca tccccaggtg gttgcgggcc gctccgaaga cgcttcccgc
    19981 gttctcggct gcttgcagcc cgataccgtg cagagcgcac cactgctccc agcgccggtg
    20041 tcgctcgaac agcggatagg tactcgcggg caatcgccag gcggtttcga ggtggccgtg
    20101 atcgaaagcc gaccccgtga cggcgagcag agtggcgtgc tcctgctcga accaggccgg
    20161 ggcttcaccg ggagaggaga agaggggcag gccgtcgggg gcttcggcgc cggggacgac
    20221 cgggaagtgc gggtgttcgg tggcctgggc gcgggcggcg gcgtgggtgt accaccgggc
    20281 gacccggccg acggcctcac tccgtgcctt cggcccgtcc tcggcctcga cgcgttcgat
    20341 cgcgtacaga cgtaccagat cgtgcaggcg gtagcggtcc tcccgcacgc gttccaccag
    20401 atgcgcgcgg gtcagccgtt ccaggaaccg ctgggcctcg tcccatcggg ccccggtgag
    20461 ccccgaggcc gccccggacg agaactcggc ccccgggtgt agccccagag cgcggaagaa
    20521 gcgggcggtg tcgtggtcca gggcacggta ggaggcgctc atcaccgcgc gcaggtcgct
    20581 cagttcgtcc tcctcggaga gcgcgtccag gcgtgactcc tcagtggcca gttcggtcac
    20641 cagatcgctc aatgacgcgt cggggcggtc gatgagccgc tcggccgcca cccgcagggc
    20701 caaggggaga cgcgcgcagt gttcggccag ttcgtgcgcc gtggtggagt cggtgtccac
    20761 acgcggaccg atcgtgttcc ggagcagtgc cacggagtcg gccatcggca ggacgtccag
    20821 ggggaggcgt cgggccccct cccgggcgat gagccccgac aacgtgttac ggctggtcac
    20881 caccaccatg cagccgggtg aggcgggcag cagcggacgc acctgagccg gtccgagcac
    20941 gtcgtcgatg acgatcagca ggtgcttgcc gtcggtctcg gtacggtaca gggccgagcg
    21001 gccgtccagg tccaaaggga cacggtcggg agggacgccg agcgcccgca ggagggagtc
    21061 caggttctgt gcggcctccg cgcgcgggcc cggggcgtgt gcgtggacgc cgacgtagag
    21121 gcagccgccg gggaatcggg ctcgggcacg gtgcgcccag tgaacggcca gcgcggtctt
    21181 gccgacaccg ggtgggccgg acagca
//
