LOCUS       NZ_CP094484            10279 bp    DNA     circular CON 27-MAR-2024
DEFINITION  Streptomyces murinus strain JS029 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP094484
VERSION     NZ_CP094484.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces murinus
  ORGANISM  Streptomyces murinus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 255578)
  AUTHORS   Chi,W.-J., Kim,K.-T., Park,S.-Y. and Kim,S.
  TITLE     Draft genome sequence of Streptomyces sp. JS029
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 255578)
  AUTHORS   Chi,W.-J., Kim,K.-T., Park,S.-Y. and Kim,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2022) Agricultural Life Science, Sunchon National
            University, Jungangro 255, Suncheon, Jeonnam 57922, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 16-MAR-2021
            Assembly Method                   :: Unicycler v. 0.3.1
            Assembly Name                     :: JS029
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 164.82x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028753175.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 03:30:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,582
            CDSs (total)                      :: 7,481
            Genes (coding)                    :: 7,273
            CDSs (with protein)               :: 7,273
            Genes (RNA)                       :: 101
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 208
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 110 of 215
            Pseudo Genes (incomplete)         :: 125 of 215
            Pseudo Genes (internal stop)      :: 20 of 215
            Pseudo Genes (multiple problems)  :: 41 of 215
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:08:47
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 103697
            Orig. end                         :: 113976
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP094484.1.
            Bacteria and source DNA available from National Institute of
            Biological Resources.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10279
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10279
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10279
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5279](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5279)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(246..1184)
                     /locus_tag="ME763_RS37215"
                     /old_locus_tag="ME763_37205"
     CDS             complement(246..1184)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018353127.1"
                     /locus_tag="ME763_RS37215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ME763_37205"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_197697424.1"
                     /transl_table=11
                     /translation="MAMIEAIGLTKQFNGKTVVEDLSFQVQPGRVTAFLGPNGAGKSTA
                     MRLMLGLDHGQGRTLFDGVPYRELRFPAQRVGAMLDAKPFHPGRSARNHLMMIAKGAGL
                     PAERADQVLDLVGLAPVVTARPCGFSLGMCQRLGLAAALLGEPDTLILDEPANGLYPQG
                     IHWLRDFLTHYAANGRSVLISSHLLAEVEAVADRLIVIGRGRLLADTSLDDFIRTFDLA
                     SVHTKTTGADDLAHTVTAEGATVVKHHRDHLVVRGLAQRRIAELASHRGLFVYELHTRT
                     ATLETAFLQASKTQTDYAAFCSPAEQEAKAR"
     gene            complement(1175..1741)
                     /locus_tag="ME763_RS37220"
                     /old_locus_tag="ME763_37210"
     CDS             complement(1175..1741)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="ME763_RS37220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ME763_37210"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_274507006.1"
                     /transl_table=11
                     /translation="MQLSATFEAPGLVHVQGGNGIGKSTLVELCSGYLRPWQGIVRVGG
                     MDAHDPRARAARRICRSQPALYPDMTVRDHLVFTTRCNGADLDTELIRAVDLGLYPWLD
                     QAARTLSTGNARKLWYVMCTTGRFNCAVLDEPFNGLDHDTTHKIATEISGWSDNRLVLL
                     VSHTLPPGLAVSDTFTLEAAGAAWR"
     gene            complement(1774..3327)
                     /locus_tag="ME763_RS37225"
                     /old_locus_tag="ME763_37215"
     CDS             complement(1774..3327)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ME763_RS37225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="ME763_37215"
                     /product="hypothetical protein"
                     /protein_id="WP_085568426.1"
                     /transl_table=11
                     /translation="MARKPMRPRHAGLRSVVDFWLFEQLNALSWSMNLGVFRLLCIGAM
                     ATLLPVVTAGALFGTRLLHTVAPLPGALPGLAFLLVHLYVGGAYVVREVFSRRRHSVSG
                     SPNEGFYRALDLRALDVFFVYCAIRIMRFYLVLLLVDAAFLYVFRESLGGAWSVGLVMP
                     VGLCALTLGVSARLATDAGAWEPSGAKALVPVGLILFVTGMVTGHLLGQGDAASHRSEH
                     AKETAAGFATGLAPDMSAGHPFVLSAGLAGAAALAAGTVLVWRAGARLRRESFVIRPAP
                     PSRAGRRSMAWLALLPLLAVVHRQFVSGRTYGPVWRIYVVVTMTLLLLIGVRLPPAGPL
                     PLSFLSQPMERSIIAVTFIVLLGGVELLLAICGPTALSPQFRFAWENLLSPRQIAVSAA
                     LYYAAHALALASGIAAIGWAATRQVLWHVPFLGLAVLSASLIAESLTTAPKRLVDGTSA
                     PGVFVACLSVALSLPALIGCLAQPVPSRLLAVVYSVALFTGAITCIGRRVQTLPLTSPA
                     "
     misc_feature    complement(2344..2346)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(3773..4375)
                     /locus_tag="ME763_RS37230"
                     /old_locus_tag="ME763_37220"
     CDS             complement(3773..4375)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ME763_RS37230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="ME763_37220"
                     /product="hypothetical protein"
                     /protein_id="WP_143657473.1"
                     /transl_table=11
                     /translation="MSVNAPAPGPVEHMKDGGGALQAVKKKWKPGPTDRLLWLVAYALT
                     VVAVLLRTPVLHGTVHQRLQASEMAGLIKDEGMETLAVNVGLLLAVLVSTLLLGIFYSL
                     AATVEKHVFTARRTVGGRSFGLFFTVALLCTLPVHAVSAALALSSPKDSGFYYLYVLVV
                     GLTVPLWFRAGWKGRGQQKALTVFAFSLGVAGLSLLI"
     gene            complement(4399..4905)
                     /locus_tag="ME763_RS37235"
                     /old_locus_tag="ME763_37225"
     CDS             complement(4399..4905)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /locus_tag="ME763_RS37235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ME763_37225"
                     /product="stage II sporulation protein M"
                     /protein_id="WP_143657474.1"
                     /transl_table=11
                     /translation="MSLAALCTGIVLGYASLASAGPADGSSGASAASAASFTAIVVRNT
                     GVAMLLFSGVATAGFSTVITLGLMATYIGATFGAAARVVGVGEALVSIVLYAPLEFVGL
                     LLAATAGTLPVVAAMASALRSGQGAPSPLHAYGTAVGLSLKTFMLAIAVVVVAAAVEAT
                     VITSR"
     gene            complement(5001..5279)
                     /locus_tag="ME763_RS37240"
                     /old_locus_tag="ME763_37230"
     CDS             complement(5001..5279)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03651"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666866.1"
                     /locus_tag="ME763_RS37240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ME763_37230"
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="WP_197697425.1"
                     /sec_met_domain="TIGR03651 (E-value: 3.1e-08, bitscore:
                     27.7, seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSFATKKTAGIALVAVVISSVGLAFAGVSVMYIAGMFGLSSAVA
                     SQIVNAVEVGGWVLAAAMALVSGGIGGTVVATAKWAISQWGKKVAIS"
     misc_feature    complement(5536..5550)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.7"
                     /tool="antismash"
     gene            5744..6331
                     /locus_tag="ME763_RS37245"
                     /old_locus_tag="ME763_37235"
     CDS             5744..6331
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /locus_tag="ME763_RS37245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ME763_37235"
                     /product="response regulator transcription factor"
                     /protein_id="WP_085568430.1"
                     /transl_table=11
                     /translation="MIVIRDAVLRRTIQDQLSEADGITTTTAADLPPGHELFPYPRPDM
                     IVLDAADDRRNALGPIARAVPLVMLIDAADHTDIAMAREVGARGILTKSMPAVQFVSAV
                     RLVAAGGDVRAPAVVARNHHRPQSRNAVPLSPREHDVLLLLADGRTNQEIAAALYLSPT
                     TVKSHVSTILRKLGVNNRVQAAAWYGQPLAEQ"
     gene            6508..>6597
                     /locus_tag="ME763_RS37250"
                     /old_locus_tag="ME763_37240"
                     /pseudo=""
     CDS             6508..>6597
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377440.1"
                     /locus_tag="ME763_RS37250"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ME763_37240"
                     /product="IS5/IS1182 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MVEWVTLLIVTREGGRCCKLPPHQCALVGL"
     gene            complement(6625..7545)
                     /locus_tag="ME763_RS37255"
                     /old_locus_tag="ME763_37245"
     CDS             complement(6625..7545)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1026: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666080.1"
                     /locus_tag="ME763_RS37255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ME763_37245"
                     /product="IS3 family transposase"
                     /protein_id="WP_274507001.1"
                     /transl_table=11
                     /translation="MGEVAAADSAQVAGVISDCKAEQDIPHTLTCRALGVSESWFYKWR
                     NQPVTAREVRRGKLADAIRQIFDESGGTYGSPKVWITLVRRGWRVSVNTVAKLMAELGL
                     AGRKIRRRRGLTRPGNRPAFADFVRRDFSATAPDQVWAGDMTEIVTSEGKLYLATVIDL
                     FSRRLLGYAMGERHDAELVVASLHMAAATRSGDVRGVIFHSDRGSEYQSRRFRRACHRL
                     GVTQSMGRVGSCFDNAVSEAFNSVLKVEYVHRRTFTTRTEARLKIATWITGFYNTRRLH
                     SVCGYRNPIDYERNHQADPTVELAA"
     gene            complement(7570..7884)
                     /locus_tag="ME763_RS37260"
                     /old_locus_tag="ME763_37250"
     CDS             complement(7570..7884)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /locus_tag="ME763_RS37260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ME763_37250"
                     /product="transposase"
                     /protein_id="WP_337999737.1"
                     /transl_table=11
                     /translation="MQGKMRFMGSKQRTYTSEFREGAVRIVIETGRPIPEVAEELGVHS
                     GTLHSWVSRWRRNGSALLRPARARCLGRPAAGHRASRTGTAAAGDEREEQADTRAGDGT
                     "
     gene            complement(<7936..8450)
                     /locus_tag="ME763_RS37265"
                     /old_locus_tag="ME763_37255"
                     /pseudo=""
     CDS             complement(<7936..8450)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377440.1"
                     /locus_tag="ME763_RS37265"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="ME763_37255"
                     /product="transposase family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MVIHCAALDLPHALVEWVTMLIVTCEGDRRCKLRPSQRAVVALVY
                     PTRTHHSREDRRRVRDQRVHGSRLHHRGR"
     gene            8748..9896
                     /locus_tag="ME763_RS37270"
                     /old_locus_tag="ME763_37260"
     CDS             8748..9896
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1206: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /locus_tag="ME763_RS37270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ME763_37260"
                     /product="ISL3 family transposase"
                     /protein_id="WP_274507002.1"
                     /transl_table=11
                     /translation="MVRALPEPEIGEVEVLGVDDFAFRKGRHYGTVLIDMATHRPLHLY
                     DGRENEDLAAWLQGHPEVKVICRDRSSGYGEGARAGAPQAQQVADRYHLWANLGQAVEK
                     TVNAHRSRLAEPLAGAEDGPGHPEVEPEVVQPSKELKIVTRLREQHAAAHELWAQGMPK
                     AAIGRKLGLHQATVRKLVGARSADDVVAKSLQRAHVVDPYVGYLHRRWNEGIRNAAQLH
                     REIQKLGFPGGELAVQRHLRRYRTGRGHAPPTVREVTSWIMSHPGHLRDGDADKLHRLR
                     ERDPELDRLTGHVRKFAVMMTARHGDRFEEWIAGVEHDTLAPLAGFARNLRRGFDAVHN
                     GLSLPHSSGAVEGNINRLKMLKRQMFGRAGLDLLRKRVLLAR"
     gene            <9935..>10092
                     /locus_tag="ME763_RS37275"
                     /old_locus_tag="ME763_37265"
                     /pseudo=""
     CDS             9935..>10092
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689697.1"
                     /locus_tag="ME763_RS37275"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="ME763_37265"
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MIRDGAVANRPIYVALAVTTEGRREIVGLWPETAVRAPSTGCTSS
                     PRSRTAA"
ORIGIN
        1 gtattcgtgc cccatggcga acacgcccag tgttccggcg acggcgggtg tcagtggtga
       61 cttgatgagc atggcgacga gggcttcgtg tccgggcacc gtggtccctg atgtggcggt
      121 catgtggctg aggagagtgc cgaaggccca ggtgagcagt gcggaggcga ggagggtggt
      181 agccgccagc caccaggtgc tgcgcagggt gcgcactcgg caccattcgt aggcgatcgc
      241 ggtcttcatc gagccttcgc ctcctgctcg gcgggcgagc agaaggccgc gtagtccgtc
      301 tgagtcttcg aagcctggag aaaggccgtt tccagggtcg cggtccgggt gtgcagttcg
      361 tagacgaaca ggccgcgatg gctggcgagc tccgcgatgc gccgctgggc caggccccga
      421 actaccagat ggtcgcgatg gtgcttgaca acggtggcgc cttcggctgt cacggtgtgg
      481 gcgaggtcat cggcaccggt ggttttggtg tggaccgagg cgagatcgaa ggtgcggatg
      541 aagtcgtcga gagaggtatc ggcgaggagc cggccacgcc cgatgacgat gaggcggtcg
      601 gcgacggctt ccacctcggc cagcagatga gaggagatga gaaccgagcg gccgtttgcg
      661 gcgtagtggg tgaggaagtc gcgcagccag tggattcctt gcgggtacaa gccgttagct
      721 ggctcgtcga ggatgagagt gtccggttcg ccaagcaggg ctgccgccag cccgagacgt
      781 tggcacatgc ccagggaaaa tccgcatggc cgcgcagtga cgaccggggc cagaccgacg
      841 agatccagga cctggtcggc ccgctcagcg ggcaggccgg cccccttcgc gatcatcatg
      901 agatggttgc gggccgagcg gccagggtga aatggcttgg cgtccagcat ggccccgacg
      961 cgttgggcgg ggaagcgcag ttcacgatag gggacgccat cgaaaagagt ccggccctgc
     1021 ccatggtcga gccccagcat gaggcgcata gccgtggatt tgccagcccc gttggggccc
     1081 aggaacgctg ttacacggcc tggttggacc tggaaggaca ggtcctcgac gaccgtcttg
     1141 ccgttgaatt gtttggtgag gccgatcgcc tcgatcatcg ccatgctgct ccggctgctt
     1201 ccagggtgaa ggtgtcgctc accgcgagtc cgggcggcag ggtgtggctg acgaggagga
     1261 ctagacggtt gtcgctccag ccggatatct cggtggcgat cttgtgagta gtgtcgtggt
     1321 ccagaccatt gaacggttcg tccaggacag cgcaattgaa gcggccggtg gtgcacatga
     1381 cataccagag tttgcgggcg ttgccggtgg acaaggtcct tgcggcctgg tcgagccagg
     1441 gatacaggcc caggtcgacg gcacgtatga gttcggtgtc caagtcggcg ccgttgcatc
     1501 gagtagtgaa aaccaggtgg tcccggacgg tcatgtccgg atagagggcg ggctgggaac
     1561 ggcagatccg gcgggctgcg cgggcgcggg ggtcatgagc gtccatgccg ccgacgcgaa
     1621 cgatgccctg ccatggacgc aggtatccgg aacagagctc caccagggtg gacttgccga
     1681 tgccgttgcc gccctggaca tgtactagcc caggtgcctc gaaggtggcc gacagctgca
     1741 tcaacacggg acggccctcg ctatagccgg ctgttacgcc ggtgaagtca agggcagggt
     1801 ctggacccgg cggccaatac atgtgatagc tcctgtgaag agggcgacgc tgtacaccac
     1861 tgcgagcagc cgcgatggaa ccggctgagc gagacagccg atgagagctg gcagcgacag
     1921 ggcaacggac aggcaggcca cgaagactcc aggggcactc gttccatcca caaggcgctt
     1981 gggagccgtg gtgagggatt cggcgatcag cgaggcagac agcaccgcga gccccaggaa
     2041 cggcacgtgc cacagcacct gcctggtcgc ggcccagccg attgccgcga taccgctagc
     2101 cagcgcgagg gcgtgcgccg cgtagtacaa cgcggcgctc acggcgatct ggcgggggga
     2161 gagcaggttt tcccaggcga agcggaactg ggggctgagc gccgtggggc cgcagatggc
     2221 cagcagcaac tcgacgcccc cgagcagaac gatgaaggtg accgcgatga ttgagcgctc
     2281 catcggctgg gacaggaagg acaacggcag cggcccagcg ggcggcaggc gcacgccgat
     2341 cagtaagagc agggtcattg tcaccaccac gtagatccgc cacaccggcc catacgtacg
     2401 gccggagaca aactgcctgt gcaccaccgc aagcagcggc agcagggcaa gccacgccat
     2461 ggaacgccgc cctgcgcggg agggtggtgc ggggcggatg acgaacgact cccgccgcag
     2521 ccgagcgccg gcgcgccaca ccaacaccgt gcccgctgcc agcgctgcgg ccccggccag
     2581 gcctgcgctc aacacgaagg ggtggccggc tgacatgtcc ggagccaggc cggtggcgaa
     2641 gcctgccgct gtctctttgg cgtgctcaga ccggtggctg gccgcatcgc cctggcccag
     2701 gagatgcccg gtgaccatgc ctgtcacgaa gaggatcaga ccgacgggga ccagcgcctt
     2761 ggctccggac ggctcccagg cccccgcgtc cgtggccagg cgggcggaca cacccagggt
     2821 cagagcacac agccccaccg gcatcacgag cccgacagac cacgcaccgc ccagcgattc
     2881 gcggaacaca tagaggaaag cggcatcgac gagcagcagc accaggtaga accgcatgat
     2941 gcggatggca cagtagacga agaacacatc aagtgccctg agatccaaag cccggtagaa
     3001 cccctcgttc ggactgccgc tgacactgtg gcggcgccgg gagaagacct cgcgcactac
     3061 gtaggcgccc cccacgtaca ggtggacgag taggaaggcc aagccaggca gcgcgcccgg
     3121 cagcggggcc accgtgtgca gcaggcgggt cccgaacagc gcgccggccg tgaccaccgg
     3181 caatagcgtt gccatcgcgc cgatgcacag aagccggaag acccccaggt tcatactcca
     3241 gctcagcgcg ttcagctgct cgaagagcca gaagtcgacg acgctgcgca ggcccgcatg
     3301 acgcgggcgc atcggtttcc tggccatttc agaaccggtt cctggaacgg ttctgcgtct
     3361 cctctgcgcg caatagcgtc acgtaacagc tcactagtac ggacgctaca acggatatcg
     3421 acaggagcca gacgagtacc accctgccgg ccgcctcacg aggtaccccg cccagtgccg
     3481 cggcatggca ggcagccgca actgggaaga cccagagcac cggccggtgc gagagcgtgc
     3541 cgagcatgag tacgcacagc agcgggacga ccgcgccgac cacggcgaat atggccgcag
     3601 gccagacggg gacggatgat gacgcaccag gcggggagtc ctgtaccacc aggccggccg
     3661 acgccgccag cccggtgaga gcgacgacca gggcggagcc tcgcaggacc actgggcgtt
     3721 ggacacagat gcgcacggat tccttcactg gcagtgtgct tgaggtagtc ggtcagatga
     3781 ggagcgagag tccggccacg cccaacgaaa acgcgaaaac ggtgagagcc ttctgctgcc
     3841 cccgcccctt ccagcccgcg cggaaccaga gcggcacagt cagcccgacc accaggacat
     3901 acaggtaata gaagcctgag tccttggggg aggagagagc cagcgcggcg ctgacggcat
     3961 gcaccggcag cgtgcacagc agcgccaccg tgaagaacag cccgaaactg cgcccgccaa
     4021 cggtgcgccg cgccgtgaaa acgtgctttt ccaccgtcgc cgcgagggaa tagaaaatcc
     4081 ccagcagcag cgtcgacacc agcacggcaa gcagcaggcc gacgttcacg gccagcgtct
     4141 ccatgccctc gtccttgatg aggccggcca tctcgctggc ctggagccgc tggtgcaccg
     4201 tgccgtgcag taccggtgtg cgcaacaaga ccgccaccac cgttagcgcg tacgcgacca
     4261 gccacagcag ccgatcggta ggacccggtt tccacttctt cttcactgcc tggagcgccc
     4321 cgcccccatc cttcatatgt tccacaggcc ccggagccgg cgcgttcacc gacatgagat
     4381 ctcctcaatt ccccccgcct accgcgaggt tatgacagtt gcctcgaccg cggccgcaac
     4441 cacgactacc gcaatggcca gcatgaacgt cttgagcgag agaccgaccg cggtgccgta
     4501 agcgtgcagc ggcgatggtg cgccctggcc gctgcgcaac gcgcttgcca tcgccgccac
     4561 cacaggcagc gtgcctgcgg tggccgccag cagcaggccc acaaactcca gcggcgcgta
     4621 cagcacgatg gataccaacg cttcgccgac acccaccacc cgggccgcag ccccgaaggt
     4681 agccccgatg tatgtggcca taaggcccag ggtgatcact gtggagaatc ccgcagtggc
     4741 caccccggag aacagcagca tcgccactcc ggtattgcgg accacgatgg ccgtgaacga
     4801 cgcggcggat gcggccgacg cgccgctgga gccatcagcc ggtccggcgg aggccaagct
     4861 cgcgtaaccc agaacgattc ctgtgcacag cgccgccaga gacaccgaga acgcaatcgc
     4921 gagcgtgcgc gtggtgacgt ccccggcggc tgtgcgtgtt gcctctgaaa tgatgcttcc
     4981 cggcagctca gttggggcag ttaggagatg gctaccttct tgccccactg gctgatggcc
     5041 cacttggcag tggccaccac ggtaccgccg atgccgccgg acaccagagc catggcggcg
     5101 gccaggaccc agccgccgac ctcgacggca ttgacgatct gcgaggccac cgcgctggac
     5161 aagccgaaca tgcccgcgat gtacatgacg ctgacaccgg cgaaggccag gccaaccgag
     5221 ctgatgacga ctgcaaccag ggcgatcccg gcagtcttct tggtggcgaa ggaactcatg
     5281 gtctcactct cgtgatcggt aacggagctg ccctgcactg aaggcccttc cctctgggtg
     5341 cccttgagta cgcgaagccc accccggcag caacgggaga cggccgcact gcagtgcggc
     5401 accagcccgc gcagcacaag agcaggtgcg ctcggggctg gacacgatcc tggcgacttc
     5461 tttgcgttgc cgtcatccgc caactgaccc cctcctggcg gatctcgagg gaaacttcct
     5521 tcccaaggga aaatgtcaaa atgaatggca cgatccatgg ggcccggggc atcgggcgta
     5581 acgaaccaag ggctggaccc ccgtgacgcg ccacctctcg cttggtacag tctttgccgt
     5641 tgattaactc tccgtagtta accgagggcg ctatgccaga agtctcccga cgactcgagc
     5701 gccgcgtgaa caaccgagta gtgggggaac agtgattact gccatgatcg tgatacgtga
     5761 cgcagtcctg cgcagaacca tccaggacca gttgtctgag gccgacggca tcaccaccac
     5821 gactgccgcc gatctgccgc ccggccacga actgtttccg tatccccggc cggacatgat
     5881 cgtgctggac gccgccgacg accggcgcaa cgccctgggg cccatcgcga gggccgtccc
     5941 gctggtcatg ctcatcgatg cggcagacca caccgacatc gccatggccc gggaggtggg
     6001 agcccgaggg atcctcacca agagcatgcc agccgtccaa ttcgtctccg ccgtaaggct
     6061 ggtggccgcc gggggagatg tccgggcccc ggcggtcgtc gccaggaatc accatcgccc
     6121 gcagtcgcgg aacgcagtgc ctttgtcgcc gcgtgagcac gacgttctgc tcctgctagc
     6181 ggacggacgc accaaccagg agatcgcggc agctctctat ctgagcccga ccacggtgaa
     6241 gagccacgtc agtacgatcc tgcgcaagct cggcgtgaac aaccgcgtgc aggctgccgc
     6301 ctggtacggc cagcctctgg ccgagcagtg aagcagccat cctcgcgtga gaagcattga
     6361 tcccgaatcc tggacagggg ttatgcggct ggtgccagcg tggttggtgt tgtttcggac
     6421 gctgtcgcgt aagcgatcgg gctgcggtag tcgaggcagg agtgaggcgt cgggtgttat
     6481 cgagccgtac tcgaccggcc tcccgccttg gtcgagtggg taaccttgct catcgtcacc
     6541 cgcgagggtg gccgatgctg caagctccca cctcaccagt gtgctctcgt cggcttgtca
     6601 atcccctcgc gccgtggaga tctttcaagc ggccaactcc acggtggggt cggcctgatg
     6661 gtttcgttcg tagtcgatcg ggtttcggta tccgcacacg ctgtgtagcc gacgggtgtt
     6721 gtagaagccg gtgatccagg tggcgatctt cagccgcgcc tcggtgcggg tggtgaaggt
     6781 gcgccggtgg acgtactcga ccttgagcac gctgttgaac gcctcgctga cggcattgtc
     6841 gaaacatgag ccgacacggc ccatggactg ggtcacgccg agtcggtggc aagcccgcct
     6901 gaagcgccgg gactggtatt cgctgccgcg gtccgagtgg aagatcacgc ccctgacgtc
     6961 gccgctgcgg gtggccgcgg ccatgtgcag ggaggcgacc accagctccg cgtcgtggcg
     7021 ctcgcccatc gcgtagccga gcaggcggcg cgagaaaaga tcgatcacgg tggccaggta
     7081 cagcttgccc tcgctggtga cgatctccgt catatccccg gcccacacct gatcgggagc
     7141 ggtggcgctg aagtcgcggc gcacgaagtc cgcaaacgcg ggccgattgc ccggcctggt
     7201 cagcccccgg cgccttcgga tcttgcgtcc ggccaggccg agttcggcca tgagcttggc
     7261 gacggtgttc accgacaccc tccagccccg gcgcacgagc gtgatccaga ccttcgggga
     7321 accatacgtg ccgcccgact catcgaagat ctgccggatc gcgtcggcca gcttcccgcg
     7381 ccgcacctcg cgagcggtga cgggctggtt gcgccacttg tagaaccagg actcgctcac
     7441 acccagcgca cggcacgtca gggtgtgcgg aatgtcctgc tcggccttgc agtcgctgat
     7501 caccccggcc acctgggccg agtccgccgc agctacttca cccacaaggc catgcagcgt
     7561 ttgaagacat cacgttccat ctccagctcg cgtatccgct tgttcttctc gctcatctcc
     7621 cgccgcagcc gttccagttc ggctcgctcg gtgtcccgca gccggccgcc cgaggcagcg
     7681 ggcgcgggcc ggtcggagga gggccgagcc gttgcgccgc caccgcgaca cccagctgtg
     7741 cagcgtcccg gagtggacac cgagctcctc ggcgacctcc gggatcggcc tgcccgtctc
     7801 gatcactatg cgtacagcac cctcacggaa ctcagatgtg tacgtccgtt gcttggaccc
     7861 cataaacctc atcttcccct gcattcacgg actccacgct acgagggagg gagagctggc
     7921 gccgatgtag acgcgatcag caattgggaa caccttggcg ttcgcagatc cggatgatcc
     7981 ggtgagtgcg ggcggctgtc aggtcgtgcg tgcgcccggg aagggcgggc gagagccaca
     8041 gcagccgacc gctggggccg gtgacgacct ggacgttcac cctgtggcgg cggtgcttgt
     8101 gggagtagtc ggcccgaccg tcgccgaccc ggtcgcactc ggcaagggtg ccgtcgagca
     8161 ggacgaagtc gggttcgtgc tcgcgaagcg tcttcagcgg gcccggcgca tgctcggcga
     8221 gcaggtcaac gaccgcggtg gtgtaggcgt gagccgtgga ctcgctgatc ccgaacccga
     8281 cggcgatcct cgcgagagtg atgtgttcgc gtagggtaca ccagtgccac caccgcgcgc
     8341 tgggagggac ggagcttgca gcgccggtcg ccctcacagg tgacgatcag catggtgacc
     8401 cactccacga gtgcatgggg aaggtcgagt gcggcgcaat ggatgaccaa cgaggccccc
     8461 gagcagcgtg atcgagacgt cagacatctc gatcaaccgc ccgggagcct cgctccttgc
     8521 gcgtcatggc ccctcacccg atcggtggcc aacccgaaga agctcagtga acctcctcca
     8581 tcctgaatag tcgcaggtca ggcgagactg taattcgttc ggtgtaactc tcgatcatgg
     8641 aagatgcatc gatggccggt gagaacgtgg ccgagcaggt ggctggggag tcggtagcgg
     8701 ctgtgtcggc gaaggcgtgg acgaccagtt gatcggcgag gctgcggatg gtccgggcct
     8761 tgcccgagcc ggagatcggt gaggttgagg tgcttggtgt cgacgacttt gccttccgca
     8821 aaggtcgcca ctacggcacg gtgctgatcg acatggccac ccaccggccg ctgcatctct
     8881 acgacggccg cgagaacgag gacctggccg cctggctcca gggccatccc gaggtgaagg
     8941 tcatctgccg ggaccgttcc agtggttacg gggaaggcgc gcgggccggg gcgccacagg
     9001 cccagcaggt cgcggaccga tatcacttgt gggccaacct tggccaggcg gttgagaaga
     9061 cggtcaacgc ccaccgctcc cgcctggcag aaccgcttgc tggagcggaa gacggccccg
     9121 gccacccgga agtggagccc gaggtggtgc agccgtcgaa ggagctgaag atcgtgaccc
     9181 ggctgcggga gcagcatgcc gccgcccacg agctgtgggc gcaggggatg ccgaaggcgg
     9241 cgatcggccg gaagctcggg ctgcaccagg ccaccgtccg caagctggtg ggcgcccgct
     9301 ccgctgatga tgtcgtcgcc aagagcctgc agcgggcgca tgtcgtcgac ccgtacgtcg
     9361 gctacctgca ccgacgctgg aacgaaggca taagaaatgc cgcacagctc caccgggaga
     9421 tccagaaact cggctttccc ggaggcgagt tggccgtcca gcgtcacctg cggcgctacc
     9481 gcaccgggcg cggccacgca ccgccgacgg tccgcgaggt cacatcctgg atcatgagcc
     9541 accccggaca cctccgtgac ggcgatgccg acaagctgca ccgcctgcgt gagcgcgacc
     9601 ccgagctgga ccggctcacc ggtcacgtca ggaagttcgc cgtcatgatg accgcacgcc
     9661 acggcgaccg gttcgaggag tggatcgcgg gcgtcgagca cgacaccctg gctccgcttg
     9721 cgggcttcgc ccgcaacctc cgccgcggct tcgacgccgt ccacaacggt ctgtccctgc
     9781 cgcatagttc aggcgctgtt gaaggcaaca tcaaccggct gaagatgctg aaacgccaga
     9841 tgttcggccg tgctggcctc gatctccttc gcaaacgcgt cctgctcgca cggtgacatc
     9901 ctctgcactg gatcacaaga tcgtcgacag aaccaagatc cgcgacggtg cggtggccaa
     9961 ccggcccatc tatgtggccc tggccgtcac caccgagggc cggcgcgaga ttgtgggcct
    10021 gtggccggag acggcggtga gggcgccaag cactggctgc acatcctcac cgagatcaag
    10081 aaccgcagca tgggtgcacc gtcgacactc cgcgctcggc tacctcacac cagccgaatt
    10141 cgaacagcgg ctgatcacgt cacgtacgct gttactcgcc gcatgaaacc cggtgtccac
    10201 tcccgggggt caacctcagt cccctgtccg ggtcctcagt gaagtgaccg aagcgcctgc
    10261 gctgttcatc gctcagaaa
//
