LOCUS       NZ_CP117709            10798 bp    DNA     linear   CON 25-MAR-2024
DEFINITION  Streptomyces sp. MMBL 11-1 chromosome, complete genome.
ACCESSION   NZ_CP117709
VERSION     NZ_CP117709.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. MMBL 11-1
  ORGANISM  Streptomyces sp. MMBL 11-1
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8003288)
  AUTHORS   Choi,S.S., Park,H.S. and Lee,H.K.
  TITLE     Whole genome sequence of Streptomyces sp. MMBL 11-1 from novel
            marine Actinomycets
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8003288)
  AUTHORS   Kim,E.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2023) MMBL, Inha university, 105, Building C, 100
            Inha-ro, Incheon, Michuhol-gu 22212, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 03-SEP-2021
            Assembly Method                   :: BLASTN v. 2.7.1+
            Assembly Name                     :: De novo
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 99.91x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028622875.1-RS_2024_03_25
            Annotation Date                   :: 03/25/2024 07:47:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,554
            CDSs (total)                      :: 7,455
            Genes (coding)                    :: 7,322
            CDSs (with protein)               :: 7,322
            Genes (RNA)                       :: 99
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 101 of 134
            Pseudo Genes (internal stop)      :: 16 of 134
            Pseudo Genes (multiple problems)  :: 36 of 134
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:00:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7548919
            Orig. end                         :: 7559717
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117709.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from professor Eung-Soo Kim
            (In-ha university).
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10798
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="32"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10798
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10798
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5798](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5798)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(323..336)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.33"
                     /tool="antismash"
     gene            complement(416..904)
                     /locus_tag="PSQ21_RS33290"
     CDS             complement(416..904)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF040274.3"
                     /locus_tag="PSQ21_RS33290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF5959 family protein"
                     /protein_id="WP_274035078.1"
                     /transl_table=11
                     /translation="MRDVLGSGEDRSVLADERAAMGDRPPRELISLADDEDNSVSVNVL
                     GRSPGWTAGLDAEIVVKTPFVSGRIDLALYVARLESWADALDRLDAGEDVAWMEMSSGP
                     SIFIQLTGERDCPEVVVEDESWSMVTVRVPLVPPDDWVADHRRRLRQVMDHWVPMLSG"
     misc_feature    903..917
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.83"
                     /tool="antismash"
     misc_feature    904..916
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.22"
                     /tool="antismash"
     misc_feature    complement(1128..1141)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.67"
                     /tool="antismash"
     gene            1217..1642
                     /gene="panD"
                     /locus_tag="PSQ21_RS33295"
     CDS             1217..1642
                     /EC_number="4.1.1.11"
                     /GO_function="GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="panD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964661.1"
                     /locus_tag="PSQ21_RS33295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aspartate 1-decarboxylase"
                     /protein_id="WP_274035079.1"
                     /transl_table=11
                     /translation="MIRTIFKSKIHRATVTQADLHYVGSVTIDAALMEAADLLPGELVH
                     IVDIDNGARLETYVIEGERGSGVIGINGAAAHLVHPGDLVILISYAQVDDAEARALVPR
                     VVHVDADNRIVALGADPSEPVPGTRTERSPQAVVANG"
     gene            1703..2056
                     /locus_tag="PSQ21_RS33300"
     CDS             1703..2056
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955645.1"
                     /locus_tag="PSQ21_RS33300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_274035080.1"
                     /transl_table=11
                     /translation="MTDKAAVEIRDDRENGSLVAYRDGEPAGTIAYFAMDPAPGALVAV
                     HTVVRPEHEGKGIAGALVREFFAMAAREGVPVVPLCPYAAQWARRHPDEAPDVPAELVQ
                     EAERQLRGHPELF"
     gene            complement(2112..2885)
                     /locus_tag="PSQ21_RS33305"
     CDS             complement(2112..2885)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PIG-L"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955646.1"
                     /locus_tag="PSQ21_RS33305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PIG-L family deacetylase"
                     /protein_id="WP_274035082.1"
                     /sec_met_domain="PIG-L (E-value: 9.8e-22, bitscore: 76.7,
                     seeds: 137, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLPSVLAVHAHPDDESLFCGGVLAQHAAAGARTAVVTATWAEGT
                     HRAAELADALDALGTGAPRLLGYADARVPESAPGRPRFLDAPLEEAVEAVVGHIRDFRP
                     EIVITADAYGGMTGHPDHVHAHRITALAVRAAALPRCCPGSGDPWQPSALYLATHPRSA
                     AVPVGGRLARPGTPADVLYCSEDAWITATVDVGPWLPRKLAAILAHRSEVERGAAPGRI
                     AALPPAVQREVLSTEWYIRRDLTPPRGGSATGLSA"
     gene            3098..3310
                     /locus_tag="PSQ21_RS33310"
     CDS             3098..3310
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PSQ21_RS33310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_073777767.1"
                     /transl_table=11
                     /translation="MTHPYPDPVPPSPTPPPVPGPPTPVPSPPPVPPGPGPVPPGPAPD
                     PIPQPPAPGPDPVPDPEPEPQPAGT"
     gene            complement(3504..4901)
                     /locus_tag="PSQ21_RS33315"
     CDS             complement(3504..4901)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_Arg_deC_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_DAP_Arg_deC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1264: diaminopimelate decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007456256.1"
                     /locus_tag="PSQ21_RS33315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminopimelate decarboxylase"
                     /protein_id="WP_274035084.1"
                     /sec_met_domain="Orn_Arg_deC_N (E-value: 1.6e-37, bitscore:
                     127.8, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 1e-07, bitscore:
                     30.8, seeds: 500, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQTTSTHHEDGTAPPAGRFDAVVRAAVGQGLLGEGSQVAGFIDT
                     EGVRASVGALHDAFAATPGVLHTFAAKACSLVPVLRLLAECGMGCEVASPGELRLAVEA
                     GFAASRIVLDSPAKTREEIRLALALGVAVNADSLDELRRVDALRDPGTESVLGLRVNPQ
                     VGGGSIGPMSTATDTSKFGVALRDPGAREAIVGAFRERPWLTRLHAHVGSQGCPLELIA
                     AGVAQTYELAEEINATLGVRQVTSLDIGGGLPVNFTDETVHPTYADYVAALTAAAPGLF
                     SGRYSLVTEFGRSLLAKNGFIGARVEYTKDAGGRRIALTHAGAQTATRTVLMPDAWPLR
                     IGAFGPDGTPKGGPPLAQDIAGPCCFAGDVVAYARELPELAEDDLVVLYDTGAYYFSTP
                     WAYNSLPRPAVYGFRTGARPADGSGKGRSDHDGPVAFVTVRDAQSLDNIAAESGLAHAD
                     ALVEPGD"
     gene            complement(5001..5798)
                     /locus_tag="PSQ21_RS33320"
     CDS             complement(5001..5798)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955648.1"
                     /locus_tag="PSQ21_RS33320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_274035085.1"
                     /sec_met_domain="Linocin_M18 (E-value: 2e-88, bitscore:
                     294.5, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPVTPSAWDEIEEEARRTFRRHVAGRRVVDVSDPDGP
                     TLAAVGDGHLTDIDPPTPDVVARARTSMPVIEWRVPFTVSRQAVDDVERGSADSDWQPV
                     KDAARTCAFAEDMAIIDGYGAAGITGLRDGSSHDPLPLPADARDYPAAVSQAVTRLRLA
                     GVDGPYRLMLGADAFTEAAETSDHGYPVKTHLSRLVDDEILWAPAVRGGVLLSARGGDF
                     ELCLGQDLSIGYADHDATSVHLYFQQAFTFRMLTPEAVVGLIA"
     gene            complement(5798..6868)
                     /locus_tag="PSQ21_RS33325"
     CDS             complement(5798..6868)
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007456253.1"
                     /locus_tag="PSQ21_RS33325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_274035086.1"
                     /transl_table=11
                     /translation="MPDAVPDPVSVQPVVAPLTSAALFLVGTIEPGGESAVRDVLPDLA
                     AVARSLGFRYPDSGLACVAGFGADAWDRLFAGPRPAHLHPFPELRGPLHHAPATPGDVL
                     LHIRAERMDVCYAWAAQLLDRLGGTLRIVDETHGFRYLDHRDLLGFVDGTENPVGDDAR
                     AAALVGADDPEFEGGSYVVVQKYLHDLTSWNALSVEEQERVIGRTKLDDVEFPDAEKPA
                     DSHLSLNTITDPDGTERDILRANMPFGSFGKGEFGTYFIGYAADPDVTERMLRNMFLGD
                     PPGTHDRILDFSTAVTGCLFFAPRADFLDAPPPPPVRTRSGDLPEATFTPVRPEPPAAG
                     ADHGSLRIGSLQESAQ"
     gene            complement(7332..7865)
                     /locus_tag="PSQ21_RS33330"
     CDS             complement(7332..7865)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964650.1"
                     /locus_tag="PSQ21_RS33330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF5709 domain-containing protein"
                     /protein_id="WP_274035088.1"
                     /transl_table=11
                     /translation="MSDTDWGDDVYQPQEPEASDPAEQLDAGDTLMDPSGTGDPLDEGY
                     APPDRPWAVDGLGTTANERHTGESLEARLTREVPEPDGSIGDGVGDLVGGDGEPWDDEV
                     GVARAGRLTQQADGSVPAALTASDVGIDGAAASAEEAAMHVIPEPGDDNALLKAGPAPG
                     TGAATGGTAAPPSP"
     gene            8278..8421
                     /locus_tag="PSQ21_RS33335"
     CDS             8278..8421
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PSQ21_RS33335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_274035089.1"
                     /transl_table=11
                     /translation="MGRSAELRTCPHRAASALPPAATRAAVPPAPADAAPGTGRTALHG
                     HG"
     gene            8679..10154
                     /locus_tag="PSQ21_RS33340"
     CDS             8679..10154
                     /EC_number="6.3.2.13"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016874 - ligase activity [Evidence IEA]"
                     /GO_process="GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /GO_process="GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955651.1"
                     /locus_tag="PSQ21_RS33340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="WP_274035090.1"
                     /transl_table=11
                     /translation="MKLSDLMAGQDHHVLRGIPARTDVTAGATFDADRVTPGSVFAAVP
                     GHAAGGPEAVAPAVARGAVAVLVDEQAPPLPEALGDVCAVRVPDVRRAAAVLASRYFGE
                     PGRAMDLIAITGTNGKTSVSYMTESVLRLAEGTRVGVIGTAGSRIGDEPIPMPPSVLTT
                     PESPDLQYLLGHMRDRATGSVVLEATSMGLLTHRLDRTFIDVGVFTNLTQDHLDDHGTM
                     ENYRDAKLRLFQGLCRRAVVNADDPVGAGIRELMPDAVTTYALDGDADYRASDLTVDAS
                     GTRFTLHHDGRKYPAAIPSPGRFSVSNALAAVAACHLLGHGLAGLVDALQRMPQIPGRF
                     ERLHTSGGTSVIVDYAHSPDSLSKVLTTIRGFAKGRVITVFGCGGDRDTTKRAEMGAIA
                     GAHSDLCVLTSDNPRFEDPEAILDEIAPGIASAGTPFERITDRRLAIAAALAEAGPADI
                     VLIAGKGSEPHQAVRGELLPFSDMATVRELIGG"
ORIGIN
        1 cggcagcaag caccacctga tcaccgacgc caccggcacc ccgctcgcca ccaccccgac
       61 cggcggcaac cgcaacgagg tcacccagct gatcccgctc ctcgaagccg ttccaccggt
      121 gcgcggcaag cgcggccggc ccaggcgccg cccggacctc gtgctcggtg accgcggcta
      181 cgaccacgac aagtaccgcc gccttgtctg ggcactcggc gtgaagccag cgatagcccg
      241 ccgcggcacc gagcacggat ccggcctggg cgcccaacgc tgggtcgtag agcacgcctt
      301 cgcccacctg cactgcttcc gccgcatgcg catccgctga gagatccgcg acgacatcca
      361 caaagccttc ccgaccctcg gatgcgtact catctgctgg cggcgacttg tcgccctacc
      421 ctgacagcat cggaacccag tgatccatca cctggcgcag acgccggcga tggtcagcaa
      481 cccagtcgtc cggcgggacg agcgggaccc gcacggtgac catggaccag gactcgtcct
      541 ccacgaccac ctccgggcag tcacgctcgc ccgtgagctg gatgaagatg gacggcccgc
      601 tgctcatctc catccaggcc acgtcttccc cggcgtcgag ccggtccagt gcgtcggccc
      661 agctctccag cctcgcaacg tacaacgcca ggtcaatacg accggacacg aaaggagtct
      721 tgacgacaat ctccgcgtcg agcccggcgg tccatccggg actacgcccc agaacgttga
      781 ccgagacgct gttgtcctcg tcatcagcga gtgagatgag ctccctcggg ggcctgtcgc
      841 ccatagctgc cctttcgtcg gcgagtaccg agcgatcctc acccgacccg aggacatccc
      901 tcatctcatt ttgctaggag ttcttatagg ggcgtggagg caggcaacca ctgtcggctc
      961 gtcgaggtga tggtgatcac tgatacccgc aggggagagc tgcgtaggct tgcggagtcg
     1021 ttccggtgga gttcgggaga gtgagtgggc cctctcgacg tcacctgacg aagcgtcagg
     1081 aaagtcagca agtggtcagc atgagtcctg aaaaacctgg ggaacgctgc ctgaacatgc
     1141 gactcgttat agggtgagga aacagccctt gacctgcaaa tacgcaggca gggagcctac
     1201 gtccaggagt acctcgatga tacgtacaat attcaagtcc aagatccacc gggccaccgt
     1261 cacccaagcc gacctacact acgtcggttc cgtgacgatc gacgccgccc tgatggaggc
     1321 cgccgacctg ctgcccggtg agctggtcca tatcgttgac atcgacaacg gcgcccggct
     1381 ggagacgtac gtcatcgagg gtgagcgcgg ctccggtgtc atcgggatca acggcgcggc
     1441 cgcacacctc gtgcaccccg gtgacctggt catcctgatc agctatgcgc aggtcgatga
     1501 cgcggaggcc cgtgccctcg tgccgagggt cgtgcacgtg gacgcggaca accggatcgt
     1561 ggcgctcggc gccgatccct cggagccggt tcccgggacc cgtacggagc ggagcccgca
     1621 ggccgtcgtc gcgaacggct gaggtccccg tccgcgcgct ggacgagcga cggccacgtc
     1681 cacgtacgag agagcgggag tcatgaccga caaggcagct gtggagatca gggacgaccg
     1741 ggagaacggc agtctcgtgg cgtacaggga cggcgagccg gccggcacca tcgcgtactt
     1801 cgccatggac ccggcccccg gtgccctggt ggccgtgcac accgtcgtcc ggcccgaaca
     1861 cgagggcaag ggcatcgcgg gggcgctcgt ccgggagttc ttcgccatgg ccgcccgcga
     1921 gggagttccc gtcgtcccgt tgtgcccgta cgcggcgcag tgggcgcggc gtcaccccga
     1981 cgaggcgccc gatgtgccgg ccgagctcgt ccaggaggcg gagcggcagc tcaggggcca
     2041 ccccgagctg ttctgacctc acacgggatc ccaccggccc ggcccgtcct cacgccgggc
     2101 ccgggagcac ctcacgcgct cagccccgtg gcggacccgc cccggggcgg cgtgaggtcc
     2161 cggcggatgt accactcggt gctcagcacc tcacgctgga ccgccggagg cagagcggcg
     2221 atccggccgg gcgcggcgcc tctctccacc tcgctgcggt gcgcgaggat cgcggccagc
     2281 tttctcggaa gccaggggcc gacgtccacg gtcgcggtga tccacgcgtc ctccgagcag
     2341 tacagcacgt cggccggggt tcccggccgg gccagccgcc cgccgaccgg aacggccgcc
     2401 gagcgggggt gcgtggcgag gtacagggcg ctcggctgcc agggatcacc ggaacccgga
     2461 cagcagcggg ggagggcggc ggcccggacc gccagcgcgg tgatccggtg ggcgtggaca
     2521 tggtcggggt ggccggtcat gcctccgtag gcgtccgccg tgatcacgat ctccggccgg
     2581 aaatcacgga tgtggccgac cacggcctcc acggcctcct ccagcggagc gtcgaggaac
     2641 cggggccggc cgggggccga ctccggcacg cgggcgtccg cgtagccgag cagccgtggg
     2701 gcgcccgtgc ccagggcatc gagggcatcg gcgagttcgg cggccctgtg cgtgccctcc
     2761 gcccaggtcg ccgtgaccac cgcggtccgg gcaccggcgg ccgcgtgctg ggcgaggact
     2821 cctccgcaga acagggactc gtcgtccgga tgggcgtgaa cggcgagaac gctcggcagg
     2881 gacacagggg gcgcctttcg cctcgacggt accggtcgcg gagccgtacg ggtccgggca
     2941 cctcgccgga gtctgcccct cggaaagacc cggcacgcgt cgccccgcgc tcctgtcgcc
     3001 gcgcgcacgt gtggactcgg caggcgcggg cagccgctcc gggagacggt ggaggaatgc
     3061 tccatctgtc cctgtcatgc cgttgtggag gatcgcgatg acccacccgt accccgaccc
     3121 cgtaccgccc tcgccgacgc ctccgcccgt accgggcccg cccactccgg tgccgagccc
     3181 gccgcccgta ccgccgggcc ccgggccggt gccgcccggg cccgcgccgg acccgatccc
     3241 gcagccgccc gcgccgggcc cggaccccgt accggacccg gagcccgagc cgcagcccgc
     3301 cgggacctga ccggcgggcc gggtccggtt caggccggca cctggcccgg tcggccgggg
     3361 cgggcagcac ccgggcggtg acggacggcc cggccctccg ccgggaggtc ggcgaagggc
     3421 cgggccgacg aggtccgcgg tgtgatcggc ccaccggacc gccccggtgc ggccgtcgcg
     3481 tcggccgggg cggccccgcc cgctcagtcg cccggctcga ccagcgcgtc cgcgtgggcg
     3541 agaccgctct ccgccgcgat gttgtccagc gactgcgcgt cccgcaccgt cacgaacgcc
     3601 accgggccgt cgtggtccga tcggcccttc cccgaaccgt ccgccggccg cgcccccgtc
     3661 cggaatccgt acaccgcggg acggggcaga ctgttgtagg cccacggggt ggagaagtag
     3721 tacgcccccg tgtcgtacag cacgaccaga tcgtcctcgg ccagctccgg cagctccctg
     3781 gcgtacgcca cgacgtcccc cgcgaagcag cacggccccg cgatgtcctg cgccagcggt
     3841 ggcccgccct tgggagtgcc gtccgggccg aacgccccga tccgcaacgg ccaggcgtcg
     3901 ggcatgagga ccgtacgggt ggccgtctgg gctcccgcgt gggtgagtgc gatccgacgg
     3961 cccccggcat ccttcgtgta ctccacccgg gccccgatga acccgttctt cgccagcagc
     4021 gaccggccga actccgtgac cagggagtag cggccggaga agaggccggg cgcggccgcc
     4081 gtcagggccg ccacatagtc cgcgtaggtg gggtgcacgg tctcgtcggt gaagttgacc
     4141 ggaagcccgc cgccgatgtc caggctggtg acctgccgga cacccagcgt cgcgttgatc
     4201 tcctcggcca gttcgtacgt ctgtgccaca ccggccgcga tcagctccaa cggacatccc
     4261 tgggagccca catgggcgtg cagccgcgtc agccacggtc gctcccggaa ggctcccacg
     4321 atcgcctcgc gggcacccgg gtcccggagc gcgaccccga atttcgaggt gtccgtcgcc
     4381 gtgctcatcg gcccgatcga cccaccgccc acctgagggt tgacccgcag tccgaggacc
     4441 gattcggtgc ccgggtcacg gagggcgtcg acgcggcgca gttcgtcgag gctgtcggcg
     4501 ttgacggcga cgccgagggc gagcgccagc cggatctcct cgcgggtctt ggcgggggag
     4561 tccagcacga tacgggacgc cgcgaaccct gcctccacgg ccagccgcag ctcaccgggg
     4621 ctcgccacct cgcaccccat cccgcactcg gccagcagcc gcagcaccgg gacgagggag
     4681 caggccttgg ccgcgaaggt gtgcagtacc ccgggcgtgg cggcgaaggc gtcgtgcagg
     4741 gccccgacgg aggcgcgcac gccctcggtg tcgatgaatc cggctacctg gctgccctcg
     4801 cccagcagcc cctgcccgac ggccgcccgg acgacggcgt cgaagcggcc ggccggcggt
     4861 gccgtgccgt cctcgtggtg cgtggaagtc gtctgcgtca tgaggccaga gtccctgatg
     4921 gctgcccccg ggtcttcggc ggaacggggg cgctcggtcg cccggccacg cgccggtgag
     4981 cgcccccggg gcatgggcga tcaggcgatc aggccgacca cggcctccgg cgtcagcatc
     5041 cggaaggtga aggcctgctg aaagtagagg tggacgctgg tggcgtcgtg gtccgcgtag
     5101 ccgatcgaca ggtcctggcc caggcagagc tcgaagtctc cgccccgggc ggacagcagc
     5161 accccgcccc tgaccgcggg cgcccacagg atctcgtcgt ccaccaggcg gctcaggtgg
     5221 gtcttgaccg gatacccgtg gtcggaggtc tcggccgcct cggtgaacgc gtccgcgccg
     5281 agcatcagcc ggtacgggcc gtcgacaccg gccagccgca gccgcgtcac cgcctggctg
     5341 accgccgccg gatagtcccg cgcgtcggcg ggcagcggga ggggatcgtg cgaggagccg
     5401 tcccgcagcc cggtgatccc ggccgccccg tacccgtcga tgatcgccat gtcctcggcg
     5461 aacgcacagg tgcgcgccgc gtccttgacg ggctgccagt cgctgtcggc ggagccgcgc
     5521 tccacgtcgt ccacggcctg gcgggagacc gtgaacggca cccgccactc gatgaccggc
     5581 atcgacgtgc gggcccgggc caccacatcc ggggtgggcg gatcgatgtc ggtcagatgg
     5641 ccgtcgccga cggcggccag ggtcgggccg tccggatcgg acacgtcgac gacacggcgg
     5701 ccggcgacat gacggcggaa ggtgcgccgg gcctcctcct cgatctcgtc ccaggcggac
     5761 ggggtgaccg gcgcgagctc gcggtgcaga ttgttcatca ctgggcgctt tcttgcaggc
     5821 tgccgatgcg cagcgaaccg tggtccgccc cggccgcggg cggctcgggc cggaccggcg
     5881 tgaacgttgc ttccggcagg tccccggagc gggtccggac gggtgggggc ggcggagcgt
     5941 cgaggaagtc ggcgcggggg gcgaagaaga ggcaaccggt gaccgctgtg gagaagtcga
     6001 ggatgcggtc gtgtgtcccg ggcggatcgc cgaggaacat gttgcggagc atccgttcgg
     6061 tcacgtcggg atcggcggcg tacccgatga agtacgtacc gaactcgccc tttccgaagc
     6121 tcccgaacgg catgttcgcc cgcaggatgt cccgttcggt gccgtccggg tcggtgatcg
     6181 tgttgagcga gaggtgggag tcggcgggct tctcggcgtc ggggaactcc acgtcgtcca
     6241 gcttggtgcg gccgatgacg cgttcctgct cctccacgga gagcgcgttc caggacgtga
     6301 ggtcgtgcag gtacttctgt acgacgacgt agctgccacc ctcgaactcc gggtcgtccg
     6361 ccccgacgag cgccgccgcc cgggcgtcgt cgcccaccgg gttctccgta ccgtccacga
     6421 agccgagcag gtcccggtgg tccaggtagc ggaagccgtg cgtctcgtcc acgatccgca
     6481 gggttccgcc gagcctgtcg agcagctggg ccgcccacgc gtagcagacg tccatccgct
     6541 cggcccggat gtgcagcagg acgtcgccgg gggtcgccgg ggcgtggtga agaggccccc
     6601 gcagttccgg gaacgggtgg aggtgagccg ggcgaggccc ggcgaacagc cggtcccagg
     6661 cgtcggcgcc gaagcccgcc acacaggcga gaccggagtc cgggtaccgg aagccgagcg
     6721 accgggccac ggctgcgagg tccggcagca cgtcccgcac cgcgctctcg ccgcccggct
     6781 cgatcgtgcc gaccaggaac agcgcggcgc tggtcaacgg cgccacgacg ggctggacgg
     6841 agacgggatc agggacggcg tcgggcatgc gcgcactcca ctacgacggt gtacggacgg
     6901 cggcgaacgg atggcggggg acggtcggcg gcgaacggtc ggcggtctac ggacggcggc
     6961 gaagagtctg ccgcggacag tcggcggtga aggggcagcg gcgaacagtc ggcggccggg
     7021 ggaccgtgac gagcagggct cgcacccact aacccatctc ccgccgccgg ccgcacgccg
     7081 ggagcatccg gccgggggag cggaggggcg ggcacctcgc gaaggtcgcg gcggccgtcc
     7141 gtcgagcgcg gcttcgggga agatcctgcg aggccgcggg ccgcctcccg cagatcgggt
     7201 gggcgcgttc ggtgccgtgt gacgcgttcg gcggtgcgtg aggggctgcg gtgcgtgagg
     7261 ggcttcggtc aggggggtgt acgggcccgg ggacggacgc gctcacgggc aggcgctcgc
     7321 cgacagcccg ctcagggcga gggcggggcg gccgtgcccc cggtggccgc accggttccg
     7381 ggggccgggc cggccttcag gagcgcgttg tcgtcccccg gctcggggat cacatgcatc
     7441 gcggcctcct cggccgacgc cgccgccccg tcgatgccca cgtcgctggc ggtgagggcg
     7501 gccgggacac tgccgtccgc ctgctgggtc agccgtccgg cgcgggcgac tccgacctcg
     7561 tcgtcccacg gctcgccgtc gccgcccacc agatcgccca cgccgtcgcc gatggaaccg
     7621 tccggctcgg gtacttccct ggtcagccgg gcttcgaggg actcgccggt gtgccgctcg
     7681 ttggccgtgg tgccgaggcc gtcgaccgcc cagggacggt ccggcggtgc gtagccctcg
     7741 tccagcgggt cgccggtgcc ggacggatcc atgagcgtgt ccccggcgtc gagctgttcg
     7801 gcggggtcgg aagcctcggg ttcctggggc tggtagacgt catcccccca gtcggtgtcg
     7861 ctcatggcgc gtcctttcct ccgggagtac cggatacccc ggataccact gtcccctgta
     7921 cgcaattcca ctccggtccc gctcccgccg caaagggaag atcggatctc ggtgtccggg
     7981 gcaggcgtcc tggaccggtc ggtacgcagg tcgcggcctg ttccgagaag cggtgcgtcg
     8041 gcgtccgggg ggtcgacgac gaggttccgg aaccgaccgt gccgccgccc ctccctcggc
     8101 accgaccgac cgaccgaccg accggccgcc agcacaccct gacgcccggc ctctgtccag
     8161 ggcccccgct ctcggcccgg ccccggcccg gcccgggact ccgttccggg cgttcgcccc
     8221 ggtcggagcg gcccgtgggc ggctcgctgc ccttcccacc gcacgtacac tcggttcatg
     8281 ggacgcagcg ccgaactgag gacatgcccg caccgggccg cctccgcgct gcccccggcc
     8341 gcgacccgcg cggctgtgcc cccggcgccc gcggacgcgg cgcccggaac gggccgtacc
     8401 gctctgcacg gccacggcta gcggtcccag cggcggttcc cgacagcggc atccggcgta
     8461 gccggccccc ggcctcaccg cccccggccc gccgtcccct cacccacacc accactccgg
     8521 cccgctgagc gccggagctc accgtcgcct ccggcgctcg ccggacgcga ccgtcacgat
     8581 cggctccaca gcaccacgcg gcccacggcg cacccgcccg tgccgcttcc cggccacggc
     8641 cgcaccagcc ggcccgcgcc gcactcgaag ggatcatcgt gaagctgagc gacctcatgg
     8701 ccgggcagga ccaccacgtg ctgcggggaa tccccgcccg cacggacgtc accgccggag
     8761 cgacgttcga cgcggaccgg gtcacccccg gatccgtctt cgccgcggtg cccggccacg
     8821 ccgccggcgg gcccgaagcc gtcgccccgg cggtcgcccg cggcgctgtc gccgtactcg
     8881 tcgacgagca ggcgccgccg ctgcccgagg cgctgggcga cgtgtgcgcc gtacgcgtcc
     8941 ccgacgtgcg cagggccgcg gcggtcctcg cctcgcgcta cttcggtgaa ccgggccggg
     9001 cgatggacct gatcgccatc accggcacca acggcaagac gtccgtctcg tacatgaccg
     9061 agtcggtgct gcggctcgcc gaggggaccc gggtcggggt gatcggcacg gcgggcagcc
     9121 gcatcgggga cgagcccatc ccgatgcccc cctcggtgct caccaccccg gaatcgcccg
     9181 atctccagta tctgctgggg cacatgcgtg accgcgccac cggcagcgtc gtcctggagg
     9241 ccacctcgat ggggctgctg acgcaccgtc tggaccgtac gttcatcgat gtcggcgtct
     9301 tcaccaacct cacccaggac cacctcgacg accacggcac catggagaac taccgggacg
     9361 ccaaactccg tctcttccag gggctgtgcc gccgagccgt ggtcaacgcc gatgacccgg
     9421 tgggcgccgg gatccgggaa ctgatgcccg acgccgtgac cacgtacgcc ctggacggcg
     9481 acgccgacta ccgcgcgagc gatctcaccg tggacgcctc gggtacccgg ttcacgctgc
     9541 accacgacgg ccgcaagtac ccggcggcga tcccctcgcc gggccggttc tcggtctcca
     9601 acgcgctggc cgcggtggcg gcctgtcacc tcctcggcca cgggctggcc gggctggtcg
     9661 acgcgctgca gcgcatgccg cagatccccg gccgcttcga acgcctccac acctccggcg
     9721 gcacctcggt gatcgtggac tacgcccact cgccggactc cctcagcaag gtcctcacca
     9781 cgatccgggg cttcgccaag ggccgggtca tcaccgtctt cggctgcggc ggcgaccgcg
     9841 acaccaccaa gcgggccgag atgggcgcca tcgcgggcgc ccactccgac ctgtgcgtcc
     9901 tcacctccga caatccccgc ttcgaggatc ccgaggccat cctcgacgag atcgcgcccg
     9961 gtatcgcgtc ggccggaacg ccgttcgagc ggatcaccga ccgtcgcctg gccatcgccg
    10021 ccgcgctggc ggaggccggg ccggccgaca tcgtcctcat cgcgggcaag ggcagcgaac
    10081 cgcaccaggc agtccggggc gagctgctgc cgttcagcga catggccacc gtgcgcgaac
    10141 tgatcggcgg ataggcggga ggggcgcgca cgcgggcgga accgcagacg ggcggaaccg
    10201 cagacgggcc gacgcgtacg cgggcagagg cggcgcacac gtacggacgg ccggagagcg
    10261 gagaccggca gaccggcaga gcgggcagcc ggtggaccgg aaggcccgaa ggcccgaagg
    10321 cccgaaggcc cgaaggcccg aaggccggaa ggccgggaga ccggggcaag atcaatccgg
    10381 tggcggtggc gccggaggtc cggcatgctg gcccggccgc gccgagcgcc ggacggggcg
    10441 ggacgagggg agtggcagcc gtggacgtct tcacgacgaa gtccgggagc gtacgggggc
    10501 gacgtgccac ccgggaccgc ggcgtcgtcg ccgtacgcgg aatcccttac gcggcaccgc
    10561 cgttcggccg cctccggttc cgggagccgc atccggtgga accctgggac ggtgtgcggg
    10621 actgcaccgc cttcgggccg gtggccccgc agtcggccga actgccgggc gcgccgagct
    10681 ggtcgcccgg tgacgaggac atcctcaccc tgaacgtctg gtcgcccgcc gacgggcccg
    10741 gtgacctgcc cgtgctggtc tggctccacg gcggcgcgta cgtcttcggc tcctcggc
//
