LOCUS       NZ_CP117709            10399 bp    DNA     linear   CON 25-MAR-2024
DEFINITION  Streptomyces sp. MMBL 11-1 chromosome, complete genome.
ACCESSION   NZ_CP117709
VERSION     NZ_CP117709.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. MMBL 11-1
  ORGANISM  Streptomyces sp. MMBL 11-1
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8003288)
  AUTHORS   Choi,S.S., Park,H.S. and Lee,H.K.
  TITLE     Whole genome sequence of Streptomyces sp. MMBL 11-1 from novel
            marine Actinomycets
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8003288)
  AUTHORS   Kim,E.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2023) MMBL, Inha university, 105, Building C, 100
            Inha-ro, Incheon, Michuhol-gu 22212, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 03-SEP-2021
            Assembly Method                   :: BLASTN v. 2.7.1+
            Assembly Name                     :: De novo
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 99.91x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028622875.1-RS_2024_03_25
            Annotation Date                   :: 03/25/2024 07:47:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,554
            CDSs (total)                      :: 7,455
            Genes (coding)                    :: 7,322
            CDSs (with protein)               :: 7,322
            Genes (RNA)                       :: 99
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 101 of 134
            Pseudo Genes (internal stop)      :: 16 of 134
            Pseudo Genes (multiple problems)  :: 36 of 134
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:00:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1398739
            Orig. end                         :: 1409138
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117709.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from professor Eung-Soo Kim
            (In-ha university).
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="10"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(370..1470)
                     /locus_tag="PSQ21_RS05960"
     CDS             complement(370..1470)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003960017.1"
                     /locus_tag="PSQ21_RS05960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="amidohydrolase family protein"
                     /protein_id="WP_274029352.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 3e-17, bitscore:
                     61.5, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDQEEQRVLRVKGRVLVGPDEVRDELWAVGGRITYERPPGADDA
                     VTVTGWALPGLVDAHCHVGLDRHGPVDAATAEQQALADREAGTLLIRDAGSPSDTRWID
                     DREDLPKIIRAGRHIARTRRYIRNYAHEIEPGDLVAYVAREARRGDGWVKLVGDWIDRD
                     AGDLTACWPRGEVEAAIAEAHRLGARVTAHCFAEDSLTDLVEAGIDCVEHATGLTEETI
                     PLFAERGVAIVPTLVNIATFPDLAAGGESKFPRWSAHMRQLHERRYDTVRAAYDAGIPV
                     YVGTDAGGSLAHGLVAGEVAELVKAGIPPLEALSATAWGARRWLGRPVLEEGAPADLVV
                     YDEDPRADVRVLAAPRHIVLNGRVTG"
     gene            1548..2654
                     /locus_tag="PSQ21_RS05965"
     CDS             1548..2654
                     /NRPS_PKS="Domain: Aminotran_5 (16-286). E-value: 2e-28.
                     Score: 91.3. Matches aSDomain:
                     nrpspksdomains_PSQ21_RS05965_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018957694.1"
                     /locus_tag="PSQ21_RS05965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_274029353.1"
                     /sec_met_domain="Aminotran_5 (E-value: 7.4e-22, bitscore:
                     76.4, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLPPLTADRFAAIERRVARLLATEQDVVITQGEALLPLE
                     GCIRGGARPGSTALNVVTGPYGQTFGNWLRDCGAEVVDLTVPFHAAVTADQVAEALAGH
                     PGIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDDWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWERIAANPAAPRRSYLSLLDWKERWIDAGRTALPHAP
                     AQLEMLGLEACVARIEDEGLDTVMARHASAAAATRAGAVALGGGLEPYVHEARDAAPVA
                     TTLRAPAGTDAAVLVAEALAADPALPLVAGGGALSKEMIRVNHYGVDATRGAVLSSLAA
                     LGAVLAEAGLPVDIEAARRAVSETWPSL"
     aSDomain        1596..2405
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PSQ21_RS05965_Aminotran_5.1"
                     /evalue="2.00E-28"
                     /label="PSQ21_RS05965_Aminotran_5.1"
                     /locus_tag="PSQ21_RS05965"
                     /protein_end="286"
                     /protein_start="16"
                     /score="91.3"
                     /tool="antismash"
                     /translation="AAIERRVARLLATEQDVVITQGEALLPLEGCIRGGARPGSTALNV
                     VTGPYGQTFGNWLRDCGAEVVDLTVPFHAAVTADQVAEALAGHPGIDFVSLVHAEAATG
                     NTNPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDDWGVDLCVIGAQKAMGGPAGVSAV
                     SVSERAWERIAANPAAPRRSYLSLLDWKERWIDAGRTALPHAPAQLEMLGLEACVARIE
                     DEGLDTVMARHASAAAATRAGAVALGGGLEPYVHEARDAAPVATTLRA"
     misc_feature    2673..2686
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.46"
                     /tool="antismash"
     misc_feature    complement(2676..2689)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.64"
                     /tool="antismash"
     misc_feature    complement(2706..2722)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.46"
                     /tool="antismash"
     misc_feature    2780..2797
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.75"
                     /tool="antismash"
     misc_feature    complement(2782..2797)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    2792..2807
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    complement(2827..2840)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 20.06"
                     /tool="antismash"
     misc_feature    2926..2938
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    complement(2938..2952)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.51"
                     /tool="antismash"
     gene            2963..3493
                     /gene="ectA"
                     /locus_tag="PSQ21_RS05970"
     CDS             2963..3493
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121394.1"
                     /locus_tag="PSQ21_RS05970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_274029354.1"
                     /transl_table=11
                     /translation="MTAAPADFARARSEFLSIDAPRVEDGAAIWRIARDSQVLDLNSSY
                     SYLLWCRDFAATSAVARGENGEPIAFVTGYIRPDRPQTLVVWQVAVDQAHRGKGLAAAL
                     LDALTARVAADQGLSSVETTITPDNTASDRLFTSYAQRHDIALEKEVLFDGGLFPEGTH
                     LPEVLYRIGPFAP"
     gene            3651..4913
                     /gene="ectB"
                     /locus_tag="PSQ21_RS05975"
     CDS             3651..4913
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-363). E-value: 1.2e-71.
                     Score: 233.5. Matches aSDomain:
                     nrpspksdomains_PSQ21_RS05975_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="PSQ21_RS05975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_274029355.1"
                     /sec_met_domain="Aminotran_3 (E-value: 8.6e-77, bitscore:
                     257.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRGWPAVFDRAQGARLTDEDGHSY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGITHGLDMATTAKRAFLETFQNVILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALQELCHRQDMLLIVDDIQMGCGRTGGFFSFEEAGIVPDIVTLSKSISGYGLPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALDAYWADGQMEKQTLARGEQVEQTLL
                     AICAEEPTAQFRGRGLVWGMEFQDPARATAVCARAFELGLLLETSGPQSEVVKLLPPLT
                     ITPEELDEGLRTLARCVRETA"
     aSDomain        3735..4739
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PSQ21_RS05975_Aminotran_3.1"
                     /evalue="1.20E-71"
                     /label="PSQ21_RS05975_Aminotran_3.1"
                     /locus_tag="PSQ21_RS05975"
                     /protein_end="363"
                     /protein_start="28"
                     /score="233.5"
                     /tool="antismash"
                     /translation="FDRAQGARLTDEDGHSYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGITHGLDMATTAKRAFLETFQNVILRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALQELCHRQDMLLIVDDIQMGCGR
                     TGGFFSFEEAGIVPDIVTLSKSISGYGLPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAALDAYWADGQMEKQTLARGEQVEQTLLAICAEEPTAQFRGRGLVWGMEFQ"
     gene            5001..5399
                     /locus_tag="PSQ21_RS05980"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045195.1"
                     /locus_tag="PSQ21_RS05980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_003969937.1"
                     /sec_met_domain="ectoine_synt (E-value: 8.9e-54, bitscore:
                     179.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFSDIENTDRHVKSASGTWESKRIVLAKEKVGFSLHETVLY
                     AGTETSMWYANHIEAVLCTEGEAELTNDETGETHWITPGTMYLLDGHERHTMRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEA"
     gene            5406..6299
                     /gene="thpD"
                     /locus_tag="PSQ21_RS05985"
     CDS             5406..6299
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156966.1"
                     /locus_tag="PSQ21_RS05985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_274029356.1"
                     /sec_met_domain="PhyH (E-value: 1.5e-37, bitscore: 128.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTEVRADLYPSRGAAEMTTPRQDPVIWSAPGAPGPVAAKDLQGY
                     EHDGFLTVDQLITPDEVAVYRAELDRLISDPAVRADERSIIEKQSQSVRSVFEVHRISE
                     VFAGLVRDERVVGRARQILGSDVYVHQSRINVKPGFGATGFYWHSDFETWHAEDGLPNM
                     RTVSVSIALTENFDTNGGLMIMPGSHKTFLGCAGETPKDNYKKSLQMQDAGTPSDEALT
                     KMADRHGIKLFTGKAGSATWFDCNAMHGSGDNITPYARSNVFIVFNSVENAAQEPFAAP
                     IRRPEFIGARDFTPVK"
     gene            6400..7446
                     /locus_tag="PSQ21_RS05990"
     CDS             6400..7446
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010070337.1"
                     /locus_tag="PSQ21_RS05990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="VOC family protein"
                     /protein_id="WP_274029357.1"
                     /transl_table=11
                     /translation="MTSHVRHITIDCADAYTLGSFWAQVLGAPLSDEDVPGDPEALVET
                     PGAAILFVQVDEAKQTKNRVHLDIQPQDRSRDEEVERLLALGATLVDDHRRPNGRGWAT
                     LADPEGNEFCVECSAAERAALSGTRLPVTADDVTSAVRLAVDVLAGAPADRWDAPAGTL
                     TWTCWETVEHLSDDLFAYAVQLGPRTPPLDGEVPYRWAPERQGGPHNAVFADRAAGPAG
                     LLATLEASGALLASMARTTPREVRSYHGYGISDPEGFAAMGVVETLVHTHDLAEGLGLA
                     WSPPPALCDRVLARLFPDAPAGGDRWAVLLWATGRAELPGHPRRTSWRWDGRPREDGRT
                     ASSAGEPV"
     gene            complement(7571..8629)
                     /locus_tag="PSQ21_RS05995"
     CDS             complement(7571..8629)
                     /NRPS_PKS="Domain: Aminotran_5 (19-347). E-value: 1.4e-24.
                     Score: 78.6. Matches aSDomain:
                     nrpspksdomains_PSQ21_RS05995_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012381431.1"
                     /locus_tag="PSQ21_RS05995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_274029358.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1e-17, bitscore:
                     62.8, seeds: 34, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.7e-05, bitscore:
                     22.8, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTILAPIQAAAAEFAPEVTYLNTSSWGLLPRRTIAAVKALADENA
                     AGRRVGAGSFDAVEAARAGFARLAGVHPDRVATGGSVTVHVGLIAASLPPGAEVLCPEG
                     EFSSVVSPFALRGDLRVRYVPLDDLAGAVRPSTALVAFSAVQSADGRVADLAAVRAAAA
                     AHGARTLLDGTQAAGWFPLEAGAYDYTVTGGFKFLLCPRGTSFLTVTEEAEDTLPPLFA
                     GWVSAGAPWAGNYGPLERLAPTARGFDEPPAFLSYHGAEHSLQLLGEVGADALHAHATG
                     LAARLRAGLAKLGHETVPGESAIVSVPGLQSRQPDLAEAGIAVSAPAGNLRISCHLYNT
                     EADVDRVLEFLA"
     aSDomain        complement(7589..8572)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PSQ21_RS05995_Aminotran_5.1"
                     /evalue="1.40E-24"
                     /label="PSQ21_RS05995_Aminotran_5.1"
                     /locus_tag="PSQ21_RS05995"
                     /protein_end="347"
                     /protein_start="19"
                     /score="78.6"
                     /tool="antismash"
                     /translation="YLNTSSWGLLPRRTIAAVKALADENAAGRRVGAGSFDAVEAARAG
                     FARLAGVHPDRVATGGSVTVHVGLIAASLPPGAEVLCPEGEFSSVVSPFALRGDLRVRY
                     VPLDDLAGAVRPSTALVAFSAVQSADGRVADLAAVRAAAAAHGARTLLDGTQAAGWFPL
                     EAGAYDYTVTGGFKFLLCPRGTSFLTVTEEAEDTLPPLFAGWVSAGAPWAGNYGPLERL
                     APTARGFDEPPAFLSYHGAEHSLQLLGEVGADALHAHATGLAARLRAGLAKLGHETVPG
                     ESAIVSVPGLQSRQPDLAEAGIAVSAPAGNLRISCHLYNTEADVDRV"
     gene            complement(8723..9439)
                     /locus_tag="PSQ21_RS06000"
     CDS             complement(8723..9439)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007450493.1"
                     /locus_tag="PSQ21_RS06000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_274029359.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYIGKARFEKGLAEFAHRDEVEVVHRSFELDP
                     SRAKGDTALVIDMLAEKYGRSREEAASMEANVAANAQAEGLGYRTEGRDHGSTFDLHRL
                     LHLAKARGRQDELLTLAYRANFAEERSVFDDEVLLALAVEAGLDADEARAVLADPGAYA
                     DDVRADEREAAELGANAVPFFVLDRRYGISGGQPSEVFVQALEQAWKDRPVTALTAVGG
                     DAAACDTDGACEVPRS"
     gene            complement(9586..10197)
                     /locus_tag="PSQ21_RS06005"
     CDS             complement(9586..10197)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018957683.1"
                     /locus_tag="PSQ21_RS06005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_274029360.1"
                     /transl_table=11
                     /translation="MSPHLDALPFALDAEGPDGRWTHADGVLTGRAGPGQDLFVPPSGD
                     SLESASDAPRLLGPVGEGDFRLVARVRPGLLSAGDAGALYLHVGDREWAKLCLEHSADL
                     PTVCTVVTRGHSDDANAFVVEEGVCWLRLSRTGSAYAFHASADGETWTFVRVFTLGTEK
                     ERAAARAGFLVQSPTGEGCGAVFDRIAFLAGGVEDLRDGR"
ORIGIN
        1 agcagggcga cggggccggc ggcgaccgcc agggcggcga gacggaaggt gttgctgttc
       61 aagatggtgg aacccccaca agagacatgg agccaccggc acagtccaat gggggacatc
      121 gcctgcgccg gtggcctcga ctccgtgaat ctttacgcac tgagggtgaa ctgacggata
      181 cctggcgtga gttcacccca aaggggggtt tccgcgcgca tattcgaatc tttcggcacg
      241 tgcgttcgcc gctgtcactc gtttcactcg gcgcgagccg tacggagcgc actcgcgccg
      301 gagcgcgcgc agaggtgctt caacgatccg ggggcggcat ccgtcaccac ccgtcaacac
      361 cgcgacgtct catccggtca cccgcccgtt cagcacgatg tgccgggggg ccgccagcac
      421 ccggacgtcc gcgcgcgggt cctcgtcgta cacgaccagg tccgccggag cgccctcctc
      481 cagcaccggc cgccccagcc agcgcctggc tccccacgcg gtggcggaga gggcctccag
      541 gggcgggatg cccgccttga ccagctcggc gacctcgccc gcgaccagcc cgtgcgccag
      601 ggagccgccc gcgtcggtgc cgacgtagac ggggatcccg gcgtcgtacg cggcgcgcac
      661 ggtgtcgtag cggcgttcgt gcagctgccg catatgcgcc gaccagcggg ggaacttgga
      721 ctcgccgccc gccgcgaggt cggggaaggt ggcgatgttg accagggtcg ggacgatcgc
      781 gaccccgcgt tcggcgaaga gcgggatggt ctcctcggtg aggccggtgg cgtgctcgac
      841 gcagtcgatg cccgcctcca ccaggtcggt gagcgaatcc tcggcgaagc agtgcgcggt
      901 gacgcgggca ccgagccggt gcgcctcggc gatggccgcc tcgacctcgc cacgcggcca
      961 gcaggcggtc aggtccccgg cgtcgcggtc gatccagtcc cccaccagct tgacccagcc
     1021 gtccccgcgc cgggcctccc gggcgacgta cgcgacgagg tcgccgggct cgatctcatg
     1081 ggcgtagttg cggatgtaac ggcgggtcct ggcgatgtga cggccggccc tgatgatctt
     1141 cggcaggtcc tcgcggtcgt cgatccaccg ggtgtcggag ggcgagcccg cgtcccggat
     1201 cagcagggtt ccggcctccc ggtcggcgag cgcctgctgc tcggcggtcg ccgcgtcgac
     1261 cgggccgtgc cggtccaggc ccacatggca gtgcgcgtcg accagcccgg gcagggccca
     1321 gccggtgacg gtcaccgcgt cgtcggcgcc gggcggccgc tcgtaggtga tccgcccgcc
     1381 cacggcccac agttcgtccc gcacctcgtc cgggccgacg agcacccggc ccttcacccg
     1441 cagcacccgc tgctcctcct gatcactcat acgcggcact gtacgaggcc gtcggtaggc
     1501 tgcctgtgcg attccgaccg tcgaacaaac ccgaagagag ccccatcgtg acgcacccct
     1561 tcctcgacct ccccccgctc accgccgacc gcttcgcggc gatcgagcgg cgcgtggccc
     1621 ggctcctcgc cacggagcag gacgtcgtca tcacccaggg cgaggcgctg ctgccgctgg
     1681 agggctgcat ccgcggcggc gcgcggcccg gctcgaccgc gctgaacgtg gtcaccgggc
     1741 cgtacgggca gaccttcggc aactggctgc gggactgcgg tgcggaggtc gtcgacctca
     1801 cggtgccctt ccacgccgcg gtcaccgccg accaggtcgc cgaggcgctc gccgggcacc
     1861 cggggatcga cttcgtctcg ctggtgcacg cggaggcggc gaccggcaac accaacccgg
     1921 tcgcggagat cggcgaggtg gtccgggcgc acggagcgct gttcatgctg gacgcggtcg
     1981 cctcggtggg cgccgagccc ctgctgccgg acgactgggg cgtcgacctg tgcgtgatcg
     2041 gcgcgcagaa ggccatgggc gggcccgccg gggtgtcggc ggtgtcggtg agcgagcggg
     2101 cctgggagcg gatcgccgcc aacccggccg ccccgcgccg ctcctacctc tccctcctgg
     2161 actggaagga gcgctggatc gacgccggcc ggacggccct gccgcacgcc ccggcgcagc
     2221 tggagatgct gggcctggag gcctgcgtgg cgcggatcga ggacgagggc ctggacacgg
     2281 tgatggcccg ccacgcctcg gccgccgcgg cgacccgggc cggagcggtg gccctgggcg
     2341 gcggtcttga gccgtacgtc cacgaggcgc gggacgcggc cccggtggcg acgacgctgc
     2401 gcgccccggc ggggacggac gcggccgtgc tggtcgcgga ggcgctcgcg gcggaccccg
     2461 cgctgccgct cgtcgcgggc ggcggggcgc tgtccaagga aatgatccgg gtcaaccatt
     2521 acggagtgga tgcgacgcgg ggcgcggtgc tgtcctcgct cgcggctctg ggggcggtgc
     2581 tggccgaagc gggcctgccg gtcgatatcg aggctgcccg gcgggccgtt tcggaaacct
     2641 ggccgagcct ctgatattca ttatgtggga agctgcttta ttaaaagcag cttcccacat
     2701 tcttcttccc gcttttccgt cacctaaacg ggccagtttt ttccggcctc gaacttcata
     2761 attcggacac atctcgtgag agttacaacc ctgtgaccga ctccacaaga tcggtgttat
     2821 gcccgattaa tccgggacaa agcggcacaa gttgccccca tgtgggggca ggtctcgcgc
     2881 acgcctgata acacatgagg gctcgccacc caaccctcta cgtcgatgca atttcgaaat
     2941 ttgctgggta aattcaattc gcatgaccgc cgcaccagca gattttgccc gtgcccgaag
     3001 cgaattcctc agtatcgatg cgccacgagt ggaggacgga gccgcgatct ggcgcatagc
     3061 ccgcgactcc caggtcctgg acctgaactc ctcgtacagc tacctgctgt ggtgccgtga
     3121 cttcgccgcg acctccgccg tcgcccgtgg cgagaacggc gagcccatcg ccttcgtgac
     3181 gggctacatc aggcccgacc gtccccagac cctcgtcgtc tggcaggtgg ccgtcgacca
     3241 ggcccaccgc ggcaagggac tggccgccgc tctgctggac gcgctgaccg cccgggtcgc
     3301 cgccgaccag ggcctgtcgt ccgtggagac gacgatcacg ccggacaaca ccgcctccga
     3361 ccggctgttc acctcctacg cccagcgcca cgacatcgcg ctggagaagg aggtgctctt
     3421 cgacggcggg ctgttccccg aagggacgca cctgcccgag gtgctctacc ggatcggccc
     3481 gttcgccccc tgagccgtcc cggcctccgc actccggcgt accccgaccc gtacgcccgg
     3541 acccaccgac tttccgcgcc gtccgaccac tcggcgcggt cccggccgca tccgtgcgcc
     3601 gggagcactc gaccactgcc cgtcacaccc cctcacgcag gagaaccgct gtgaccatca
     3661 ccccgcccgc actgagcgtc ttcgagaccc tggagtcgga ggtacggagc tactgccgcg
     3721 gctggcccgc cgtcttcgac cgcgcgcagg gcgcccggct gaccgacgag gacggccact
     3781 cctacctgga cttcttcgcc ggggccggct cgctcaacta cggccacaac aacccggtgc
     3841 tgaaacgagc gctgatcgac tacatcgagc gcgacggcat cacccacggc ctggacatgg
     3901 cgaccaccgc caaacgcgcg ttcctggaga cgttccagaa cgtgatcctg cggccgcgcg
     3961 acctgccgta caaggtgatg ttccccggcc cgaccggcac caacgccgtc gagtcggcgc
     4021 tgaagctggc ccgtaaggtc aagggccgcg agtccgtcgt ctcgttcacc aacgccttcc
     4081 acggcatgtc gctgggctcg ctcgccgtga ccggcaacgc gttcaagcgg gccggggccg
     4141 gtatcccgct ggtgcacggc accccgatgc cgttcgacaa ctacttcgac ggtacggtcc
     4201 ccgacttcct ctggttcgag cgactgctgg aggaccaggg ctccgggctc aacaagcccg
     4261 ccgcggtgat cgtggagacg gtccagggag agggcggcat caacgtcgcc cgcgccgagt
     4321 ggctgcgcgc gctgcaggag ctgtgccacc gccaggacat gctgctgatc gtcgacgaca
     4381 tccagatggg ctgcggccgt accggcggct tcttctcctt cgaggaggcc ggcatcgtcc
     4441 cggacatcgt cacgctctcg aagtccatca gcggctacgg cctgccgatg tcgctgtgcc
     4501 tgttcaagcc ggagctggac atctgggagc cgggcgagca caacggcacc ttccgcggca
     4561 acaacccggc cttcgtcacg gccgccgccg cgctggacgc ctactgggcc gacggccaga
     4621 tggagaagca gaccctggcc cgcggcgaac aggtcgagca gacactgctg gccatctgcg
     4681 ccgaggagcc gaccgcgcag ttccgcggcc ggggcctggt ctggggcatg gagttccagg
     4741 acccggcgcg cgccacggcg gtctgcgccc gcgccttcga gctggggctc ctgctggaga
     4801 cctcgggccc gcagagcgag gtcgtcaagc tgctgccgcc gctgaccatc acccccgaag
     4861 agctggacga gggcctgcgc acgctggccc gctgcgtccg cgagacggcc tgacccggca
     4921 ctgccggggc aggcggagaa gagaggtccc gggcccgcga ggcccgggac cccgtacaga
     4981 gagaaaggca accgctcacc gtgatcgtcc gatcgttcag tgacatcgag aacaccgacc
     5041 ggcatgtgaa gtcagcgtcc ggcacctggg agagcaagcg cattgtgctc gccaaggaga
     5101 aggtgggctt ctcgctccac gagaccgtgc tgtacgcggg cacggagacc tccatgtggt
     5161 acgcgaacca catcgaggcg gtgctctgca ccgagggcga ggccgagctc accaacgacg
     5221 agaccggcga gacgcactgg atcacgcccg gcaccatgta cctgctggac gggcatgagc
     5281 ggcacaccat gcggcccaag accgacttcc gctgcgtgtg tgtcttcaac ccccccgtca
     5341 ccggacggga ggaccatgac gagaacggtg tctatccact gctgaccgag gaggcctgaa
     5401 ccaccatgac caccgaagta cgcgccgatc tgtacccctc gcgcggcgcc gccgagatga
     5461 ccactccccg ccaggacccg gtcatctggt ccgcgccggg cgcaccgggc ccggtcgccg
     5521 ccaaggacct ccagggatac gagcacgacg gcttcctcac cgtcgaccag ctcatcaccc
     5581 cggacgaggt cgccgtctac cgggccgagc tggaccggct gatctccgat ccggcggtcc
     5641 gcgccgacga acgctcgatc atcgagaagc agtcgcagtc cgtacggtcc gtcttcgagg
     5701 tccacaggat cagcgaggtc ttcgccggtc tggtccgcga cgagcgggtg gtgggccggg
     5761 cccgccagat cctcggttcg gacgtgtacg tccaccagtc ccggatcaac gtgaagccgg
     5821 gcttcggcgc cacgggcttc tactggcact cggacttcga gacctggcac gcggaggacg
     5881 gtctgccgaa catgcggacg gtgtccgtgt cgatcgcgct gaccgagaac ttcgacacca
     5941 acggcgggct gatgatcatg cccggttcgc acaagacgtt cctcggctgt gcgggcgaga
     6001 cgccgaagga caactacaag aagtcgctcc agatgcagga cgccggcacc ccgtccgacg
     6061 aggcgctgac gaagatggcc gaccgccacg gcatcaagct cttcacgggc aaggccggtt
     6121 cggcgacctg gttcgactgc aacgcgatgc acggctcggg cgacaacatc accccgtacg
     6181 cgcgcagcaa cgtcttcatc gtcttcaaca gcgtggagaa cgcggcgcag gagcccttcg
     6241 cggctccgat ccgccgcccc gagttcatcg gggcgcggga cttcaccccg gtgaagtagc
     6301 ggcacgcacg ggcgggagag ggccgggacg gtacgccgtc ccggccctct ccgcgtaact
     6361 cgttggaccg gggccgggtc agcggagagg gtgtccgcca tgacctccca tgtacgccac
     6421 atcacgatcg actgcgccga cgcctacacg ctcggcagct tctgggccca ggtgctcggc
     6481 gcgccgctct ccgacgagga cgtccccggc gatccggagg cgctggtgga gacccccggc
     6541 gccgcgatcc tcttcgtcca ggtggacgag gcgaaacaga ccaagaaccg cgtccatctg
     6601 gacatccagc cgcaggaccg ctcccgggac gaggaggtgg agcggctgct cgcgctcggc
     6661 gcgaccctgg tggacgacca ccgccggccg aacgggcgcg gctgggcgac gctcgccgat
     6721 cctgagggca acgagttctg cgtggagtgc tccgccgccg agcgggcggc cctgagcggg
     6781 acccggctgc cggtgaccgc cgacgatgtg acctcggcgg tgcggctcgc ggtggacgtg
     6841 ctggccgggg ccccggcgga ccgctgggac gcgcccgcgg ggaccctcac gtggacctgc
     6901 tgggagacgg tggagcacct gagcgacgat ctgttcgcgt acgccgtgca gttgggcccg
     6961 cgtacgcctc cgctggacgg cgaggtgccc taccggtggg cgcccgaacg gcagggcggg
     7021 ccgcacaacg cggtcttcgc cgaccgtgcc gccggtcccg ccggactgct ggccacgctg
     7081 gaggcgagcg gggcgctgct ggcctcgatg gcgcggacga cgccgcgcga ggtgcgctcg
     7141 taccacgggt acgggatctc cgacccggag ggcttcgccg cgatgggtgt ggtggagacc
     7201 ctggtgcaca cgcacgatct cgccgagggc ctggggctgg cctggtcgcc gcccccggcg
     7261 ctctgcgacc gggtgctggc ccggctcttc ccggacgccc cggcgggcgg cgaccggtgg
     7321 gccgtactgc tgtgggccac cggccgcgcc gagctgccgg gccacccgcg ccggacctcg
     7381 tggcggtggg acgggaggcc gcgcgaggac ggtcggacgg cgtcgagcgc cggggagccg
     7441 gtgtagcccc tggcgtcgcc gtcgaaaaag gccgacgggc gggtcggact ccgggatgtg
     7501 cgggcgggcg atgccgagcg gctcggtcgc ccgcaccgcc cgaccggtgc cgagccgcgc
     7561 tccgtacgga tcaggccagg aactccagca cccggtccac atcggcctcc gtgttgtaga
     7621 gatggcagga gatccgtagg ttcccggccg gggccgagac ggcgatgccg gcctccgcga
     7681 ggtcgggctg acggctctgc agtcccggca cggagacgat cgccgactcc ccgggaacgg
     7741 tctcgtgccc gagtttcgcc agtcccgccc gcagccgggc cgcgagcccg gtggcgtgcg
     7801 cgtggagggc gtcggccccc acctcgccga gcagctgcag ggagtgctcg gccccgtggt
     7861 acgagaggaa tgcgggcggc tcgtcgaaac cgcgggcggt gggggcgagg cgctccagcg
     7921 ggccgtagtt gcccgcccag ggcgccccgg cggagaccca gcccgcgaag aggggcggca
     7981 gggtgtcctc cgcctcctcg gtgacggtca ggaacgacgt accgcgtggg cagagcagga
     8041 acttgaagcc gccggtcacg gtgtagtcgt acgcccccgc ctccagcggg aaccagcccg
     8101 ccgcctgggt tccgtccagc agggtccggg caccgtgcgc cgcggcggcc gcgcggacgg
     8161 cggcgagatc ggccaccctg ccgtctgccg actgcacggc ggagaaggcg acgagggcgg
     8221 tcgaggggcg tacggccccg gcgaggtcat cgagcggcac gtaccgcacg cgcagatcgc
     8281 cgcggagcgc gaacgggctg accacggagg agaactcgcc ctcggggcag aggacttcgg
     8341 cgcccggcgg cagcgaggcg gcgatcaggc cgacgtggac ggtgacggac ccgccggtgg
     8401 cgacccggtc cgggtggact ccggcgagcc gggcgaaacc ggcccgggct gcctccaccg
     8461 cgtcgaagct gcccgccccc acccggcgcc ccgcggcgtt ctcgtcggcc agggccttga
     8521 cggcggcgat cgtccggcgg ggcagcaggc cccagctgga ggtgttgaga taggtgacct
     8581 cgggcgcgaa ttcggcggct gccgcttgga tgggcgcaag gatcgtcata cgtccaccct
     8641 ggagccaccc tcccccaaag tcaatcacgg agagccaagg agatatcaaa ggactactta
     8701 tggatggctc ccgggtgcgg gctcagctcc gcggaacctc gcaggcgccg tccgtgtcac
     8761 aggccgccgc atccccgccg acggcggtga gcgcggtgac ggggcgatcc ttccaagcct
     8821 gctccagcgc ctggacgaag acctccgacg gctggccgcc ggagattccg taccggcggt
     8881 cgagaacgaa gaacggcacg gcgttggcac ccagttcggc cgcctcgcgc tcgtcggcgc
     8941 ggacgtcgtc cgcgtacgcc ccggggtcgg cgagcaccgc acgggcctcg tcggcgtcga
     9001 gtccggcctc cacggcgagg gcgagcagca cctcgtcgtc gaagacggac cgctcctcgg
     9061 cgaagttcgc ccggtaggcg agggtcagca gctcgtcctg gcggccgcgg gccttggcca
     9121 ggtgcagcag ccggtggagg tcgaaggtgg agccgtggtc acggccctcg gtgcggtagc
     9181 cgagcccctc ggcctgcgcg ttggccgcga cgttcgcctc catcgaggcc gcttcctcgc
     9241 ggctgcggcc gtacttctcg gccagcatgt cgatcacgag cgcggtgtcg cccttggccc
     9301 gcgaggggtc gagctcgaag gagcggtgca ccacctcgac ctcgtcccgg tgggcgaact
     9361 ccgcgaggcc cttctcgaag cgggccttgc cgatgtagca ccacgggcag gcgatgtcgc
     9421 tccagatctc gacgcgcatg actcttcttc tccctgggga acgtggacgg agtgcgcggt
     9481 gtcccgcact cgattcggca acgtcgtcgc ggcaccggac attccggagg aaggagccga
     9541 gccggtgacg acagggcggt caccaccgca ccgcgcgccc gcccgtcacc gcccgtcgcg
     9601 caggtcctcc actcccccgg ccaggaacgc gatccggtcg aagaccgcgc cgcagccctc
     9661 gccggtgggc gactgcacca ggaagccggc gcgggcggcg gcccgctcct tctccgtgcc
     9721 gagggtgaag acgcggacga aggtccacgt ctcgccgtcg gcggaggcgt ggaaggcgta
     9781 ggcgctgccg gtgcggctga ggcggagcca gcagacgccc tcctccacca cgaaggcgtt
     9841 ggcgtcgtcg ctgtggcccc gggtgacgac ggtgcagacg gtcgggaggt ccgcggagtg
     9901 ctccaggcag agtttggccc actcgcggtc cccgacgtgc agatacagcg cgcccgcgtc
     9961 accggccgac agcagccccg ggcgcacccg ggccaccagc cggaagtccc cctcccccac
    10021 cgggccgagc aggcgcgggg cgtcggaggc cgactccagg gagtcgccgg acggggggac
    10081 gaacaggtcc tggccgggtc cggcccggcc cgtcagcacg ccgtcggcgt gggtccagcg
    10141 tccgtcgggg ccctccgcgt ccagggcgaa ggggagggcg tccaggtgcg ggctcatgcg
    10201 cggcgcccgt ccacccggcg gggcaggccc agcgggttgt cctcgcgcag ctccggcggg
    10261 aggagcgcct cgggggtgga ctggaacgtg acggggcgca gccagcgttc gatcgccgtc
    10321 gcgccgacgg aggtggaggt ggaggtggtg gccgggtagg ggccgccgtg gtgctgagcg
    10381 ggggcgacgg cgacgccgg
//
