LOCUS       NZ_CP117709            10945 bp    DNA     linear   CON 25-MAR-2024
DEFINITION  Streptomyces sp. MMBL 11-1 chromosome, complete genome.
ACCESSION   NZ_CP117709
VERSION     NZ_CP117709.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. MMBL 11-1
  ORGANISM  Streptomyces sp. MMBL 11-1
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8003288)
  AUTHORS   Choi,S.S., Park,H.S. and Lee,H.K.
  TITLE     Whole genome sequence of Streptomyces sp. MMBL 11-1 from novel
            marine Actinomycets
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8003288)
  AUTHORS   Kim,E.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2023) MMBL, Inha university, 105, Building C, 100
            Inha-ro, Incheon, Michuhol-gu 22212, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 03-SEP-2021
            Assembly Method                   :: BLASTN v. 2.7.1+
            Assembly Name                     :: De novo
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 99.91x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028622875.1-RS_2024_03_25
            Annotation Date                   :: 03/25/2024 07:47:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,554
            CDSs (total)                      :: 7,455
            Genes (coding)                    :: 7,322
            CDSs (with protein)               :: 7,322
            Genes (RNA)                       :: 99
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 101 of 134
            Pseudo Genes (internal stop)      :: 16 of 134
            Pseudo Genes (multiple problems)  :: 36 of 134
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:00:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 34961
            Orig. end                         :: 45906
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117709.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from professor Eung-Soo Kim
            (In-ha university).
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10945
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10945
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10945
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5945](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5945)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    336..350
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
     misc_feature    complement(433..442)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            complement(1605..2645)
                     /locus_tag="PSQ21_RS00170"
     CDS             complement(1605..2645)
                     /EC_number="1.1.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (19-269). E-value: 6.4e-13.
                     Score: 40.4. Matches aSDomain:
                     nrpspksdomains_PSQ21_RS00170_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019761872.1"
                     /locus_tag="PSQ21_RS00170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="WP_274028244.1"
                     /sec_met_domain="ADH_N (E-value: 3.3e-28, bitscore: 96.4,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 5.9e-23, bitscore:
                     80.0, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVAAYAAPRAKAPLERTTIERRPVGEFDVLIDIKFAGICHSDI
                     HQARDGWGEGIFPMVPGHEIAGIVAETGPGVTRFKVGDRVGVGCMVDSCGTCDACLLGR
                     EQHCAEGNTQTYNALDRGGEPTYGGYSTHIVVAETFALSIPEGIALDEAAPLLCAGITT
                     YSPLKRWGAGPGKKVAVVGLGGLGHMAVKIAHALGAEVTVLSQSLRKKDDGLKLGADHY
                     YATSDPRTFEELAGTFDVILSTVSAPLDLGAYLALLRTDGTLVNVGAPEEPISLNLFSL
                     ILGNKSIAGSMIGGIKETQEMLDFCAVHGLGAEIEVIGAGQVNEAYERVLASDVRYRFV
                     IDTATI"
     aSDomain        complement(1839..2588)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PSQ21_RS00170_PKS_ER.1"
                     /evalue="6.40E-13"
                     /label="PSQ21_RS00170_PKS_ER.1"
                     /locus_tag="PSQ21_RS00170"
                     /protein_end="269"
                     /protein_start="19"
                     /score="40.4"
                     /tool="antismash"
                     /translation="TIERRPVGEFDVLIDIKFAGICHSDIHQARDGWGEGIFPMVPGHE
                     IAGIVAETGPGVTRFKVGDRVGVGCMVDSCGTCDACLLGREQHCAEGNTQTYNALDRGG
                     EPTYGGYSTHIVVAETFALSIPEGIALDEAAPLLCAGITTYSPLKRWGAGPGKKVAVVG
                     LGGLGHMAVKIAHALGAEVTVLSQSLRKKDDGLKLGADHYYATSDPRTFEELAGTFDVI
                     LSTVSAPLDLGAYLALLRTDGTLVNVGA"
     gene            2808..3737
                     /locus_tag="PSQ21_RS00175"
     CDS             2808..3737
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459219.1"
                     /locus_tag="PSQ21_RS00175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_274028245.1"
                     /transl_table=11
                     /translation="MDDRPEGAAPEPLDGRAELSEFLRTRRARLQPGDVGLPEFGRHRR
                     VPGLRREELAQLAGVSVAYYTRLEQGNGRNVSAEVLDAIARALRLTDAESAHLTHLAKP
                     ARHRKKRRPARVQRVRKGLLYLLDSMDGIPAYVTGARSDVLAWNPMAAAVFGDWGALPP
                     GERNWARLVFLSPDYRDLFANWDSKASDMVSYLRLYAGCHPDDPELSALVGELSVKSDE
                     FRRLWATHNVKEKGHGSKVLRHPLVGELTLSYETLNLPDDEEQHLVTYHAEPGSESYQA
                     LRLLASWGADAGRPGAVSEAGRGPAGER"
     gene            complement(3700..>3905)
                     /locus_tag="PSQ21_RS00180"
                     /pseudo=""
     CDS             complement(3700..>3905)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377735.1"
                     /locus_tag="PSQ21_RS00180"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="SAM-dependent methyltransferase"
                     /pseudo=""
                     /transl_table=11
                     /translation="RWTYVFRSDGQAGRSSPPTPPCASADVRGPRRTWRHRGSSYVTCA
                     RRPAAPAGSTSSSLAGRTSARLA"
     gene            complement(4140..4988)
                     /locus_tag="PSQ21_RS00185"
     CDS             complement(4140..4988)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012382293.1"
                     /locus_tag="PSQ21_RS00185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)H-binding protein"
                     /protein_id="WP_274028246.1"
                     /transl_table=11
                     /translation="MILVTGATGAVGREVAGQLAAAGPVRILARRPERLTVQGGGVEIV
                     EGEYGDRAALDRALRGVASVFLVTNSPTEPDDERVAAAAAAAGVRHLVKLSMMAVEEPG
                     ADDFITRRQRENEQAVRESGVPWTFVRPRTFMSNTLSWGPGIRSAGVVRALYGDAPVAC
                     VDPRDVAAVAVAVLTGTGHEGRAYAVSGPEAITAREQTAQLSRVLGRPLRFEELDLDTA
                     RTALLAKYPPPVAEAFLRSAERQRAGAKAAVVPTVQELTGRPARPYRVWSAEHAEAFTP
                     E"
     gene            complement(5001..5945)
                     /locus_tag="PSQ21_RS00190"
     CDS             complement(5001..5945)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971211.1"
                     /locus_tag="PSQ21_RS00190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_274028247.1"
                     /sec_met_domain="AfsA (E-value: 1.6e-25, bitscore: 87.6,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPEAAVLIDSVPTMDAEAEVVHSVGIDMVHRTRPEDAFPRNWVRL
                     EHDRFSVGAVLPHDHPFFAPVGDDRHDPLLVAEAMRQAAMLAFHAGYGIPLGYQFLMTG
                     LDYVCHQEHLAVGGAPTEIDVEVFCSDLKWRGGLPAQGRVGWAVYRDGRLAATGRGETR
                     FSTPKAYRRMRGDAPAEGISIPETVPVPAVRAGRTRVEDVVLSETGRDGVWELRVDTRH
                     PTLFQRPNDHVPGMLLLEAARQAACLAAGPAGIVPAEARTRFHRYSEFGSPCWIRAVVL
                     PGTAEDTVTVQVTGHQDGETVFSTVLSGPRAHG"
     misc_feature    5994..6007
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.43"
                     /tool="antismash"
     misc_feature    5996..6012
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 17.01"
                     /tool="antismash"
     gene            complement(6481..7092)
                     /locus_tag="PSQ21_RS00195"
     CDS             complement(6481..7092)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459226.1"
                     /locus_tag="PSQ21_RS00195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="WP_274028248.1"
                     /transl_table=11
                     /translation="MTNALTTAAAGFGTGLSLIVAIGAQNALVLRQGVRRDGVLAVVGI
                     CALSDAVLIALGVAGVGALVVAWPSALTVVGLLGGVFLIGYGLLAARRVLRPSALHAGD
                     APAASRGSVVLTCLAMTWLNPHVYLDTVFLLGSVAADRGPLRWTFGLGAILASVCWFAA
                     LGFGARLLSGVLARPSAWRVLDGLVAVMMIGLGAKLALNA"
     misc_feature    7147..7161
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.4"
                     /tool="antismash"
     gene            7160..8080
                     /locus_tag="PSQ21_RS00200"
     CDS             7160..8080
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1031: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971209.1"
                     /locus_tag="PSQ21_RS00200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="WP_274028249.1"
                     /transl_table=11
                     /translation="MMSELPLDQVRTLLAVVDEGTFDSAANALRLTPSAVSQRVKALEQ
                     RTGRVLLMRTKPVRPTESGEVVVRFARQLARLEQDAQAELGMSGPGEPTLLPIAVNSDS
                     LATWFLPALRRVPEELGLCYELRREDQDHTAALLREGLAMAAVTSSPEAVTGCSVRPLG
                     RMRYLPVASPGFADRWLGRREGTALRELIGDAPVIGFDRRDDLQDAFVRRLGPGRRPSP
                     RRHLVPTSEGFAHAVASGMGWGMVPEVQAEPLLSGGRLVRLTPDHTVDVPLYWQQWKLE
                     SPALAAVAEAVVAEAAEALDGDWPD"
     gene            8187..9047
                     /locus_tag="PSQ21_RS00205"
     CDS             8187..9047
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971205.1"
                     /locus_tag="PSQ21_RS00205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="WP_274028250.1"
                     /transl_table=11
                     /translation="MPQPAVRPFLRRGSTLAGLAVGVATATVAGLLLSDTFASGTTADA
                     APEVVPPEANVTFDYQIGGGYSPPAGVGAVSRDRRDEPAEGLYNVCYVNAFQTQPDALD
                     QWEKNHPDLLLRDGDGRLVNDEDWGEALLDTSTADKRARLADIVGGWVDGCAKSGFQAV
                     EPDNLDSFERSEGLLTREHNAAFAELLAHRAHAAGLAIGQKNTTGLLERRAEIGFDFAV
                     AEECGRYDECADYASAYGDRVFVVEYTDGDFAKACSSVGTTVSVVRRDLDVRPAGQPGY
                     VFRTC"
     gene            complement(9072..9869)
                     /locus_tag="PSQ21_RS00210"
     CDS             complement(9072..9869)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455765.1"
                     /locus_tag="PSQ21_RS00210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="VOC family protein"
                     /protein_id="WP_274028251.1"
                     /transl_table=11
                     /translation="MTAAEQSTPVHGVPCWVSLMTRDLRATKDFYGTVLGWTFRSGTLG
                     EGFTVAHADALPVAGIGQIAHGLPTEVSWTPYFAVADVDATAARIRERGGTIAVGPIRV
                     GPGRGAVAADREGAGFGFWEGRTPPWSFGRDHPPASIELRTSHAFDCAIFYAEVFGWAS
                     GEPGGCEVGYAYQQDTVVVRQGGRTVATLRGGGDASSPDPRLRPRWHVRFPVADLEAAT
                     EAARAAGGTGGPPVDSPADGTEAVIRDPDGGIFTICAHPTGAP"
     misc_feature    complement(10046..10062)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.03"
                     /tool="antismash"
     gene            complement(10264..10929)
                     /locus_tag="PSQ21_RS00215"
     CDS             complement(10264..10929)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1141: acyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956398.1"
                     /locus_tag="PSQ21_RS00215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="WP_274028252.1"
                     /transl_table=11
                     /translation="MLSRLAAAVVPSLGRLTVTSDHATAPAAGSIIVANHTSLADPGIV
                     LAALLRLGVEPVVMATAGLWRVPVLGRLLERGGHVPVHRNTDRAADALDTAAAALRDGR
                     HLLIYGEGRLPRRTDAAEAPPENFRSGLARLAHASGAPVVPLGQAGARRVTSGSCVKQI
                     SGILTAPARRPRLHVHVGAPLQLPPEVEAATVTAREAVTAAWRTAAHRLGEPAALAGH"
ORIGIN
        1 cgcgaccagg aggacgcggg cgtcgtggct gatacgggtg ggggcttccc gtggcgcgtc
       61 ccagaaggcg caggggccga tgttctcggt gagggggccg aacagggggt gtgcgccacg
      121 gctccgttgg atgtcgcgcc agtagcgctc ggtctcgcgc ggggccggta cgtcaccgca
      181 gatgatcgcc gtctgggagc tgccgaagga ggagcccgcg ccggagaaca ggaggcgcag
      241 cgtctccgcg aaccccgggg gcaacggagc ctcacggccc ttggacgccc gggacagtgc
      301 cgccacctgc tgggcaagga ggtcccgcgc gccgtcgacg tcgtcggcca cgcccatgaa
      361 gagcagcgtc ggtatgtggg tgtcgtcgac ccggaagacg tcggggccgg tgccgagcgt
      421 cagcggggcg cgggccgcct tcgccatgac gtggtcgatg gtggagagca ccgcggtacg
      481 ggagcggccc aggccgtacg tcgtgtggcg ggcggccgcc caggcggccc aggcggagag
      541 cgcctgctcg ttctcgcgca cggtgttctt cagcaggcgc gggttgtagt cgtccggacc
      601 caccgctccg tccaggacca tccggtcgta gcggccgggg aacatctggg tgtagaccgt
      661 gcccaggtag gtgccgtacg aatagcccag gtaggagatc ttgcgctcgc ccagtgcgcc
      721 ccggatcacg tccatgtccc tggctgtgtt gcgggtgctg atgtgcggga gcaccgaggc
      781 gttcgtggcg cggcacttgt cggccaggct cttgctcagc gccacctggc ggtcgaagcc
      841 ggcccggctc gcaccggccg agaaccaggt gaagctgacg ggccagccgc agtccagcgg
      901 ggtgctccgg ccgacgaagc gcgggtcgaa gccgacgatg tcgtagcgcg ggccgacctc
      961 cttcatcgcc gtgcggacga tcggcgggga atccacggcc gggccgccgg ggccgccgtt
     1021 gttgaggagg atcgcgccga tgcggcggcg ggtgtcggtg gccttcagac gggatatcgc
     1081 gaccgtgatc gtccggcccc cgggggctgt gtagtccagc gggaccgtga cgtcggcaca
     1141 ctgcacgccc gccctgtcca ggtctctgcc cgtctcgtcc tgcggggagc tcatacaggt
     1201 gccccagtcc aggggttggt ggtaatagcg gtcgagcgct ccggcggtcg tcggcggtgc
     1261 gaccgtacgg ggtaccggcc cgggccgtgc ggtggccagg gccggtgacg ccgtgatgcc
     1321 cgccgcgagg gccagggcca ggcccgcggc ggcggccctg gcggtcgtcc gggcggccgg
     1381 ggtggtgcgg gcggccgccc ggggtcggat ggtcttctct gcgtcctgtg cgtaactcac
     1441 atcaatggac gctaggggaa ggaccgttgg ggatcattgg ggctggcacc ccaacgcggc
     1501 ggggggtcgc ctcccccttt cgtcgggttc ccgggtccag gaccgcctgt ccactcccgc
     1561 aggggcaggg tggtggacgt gcgcgtggcg gggcggccga cgcctcagat cgtcgccgtg
     1621 tcgatgacga agcggtagcg gacgtcgctg gcgagaaccc gctcgtacgc ctcgttgacc
     1681 tggcccgcgc cgatcacctc gatctcggcg ccgagcccgt gcacggcgca gaagtccagc
     1741 atctcctggg tctccttgat cccgccgatc atcgaacccg cgatcgactt gttgcccagg
     1801 atcagggaga agaggttcag cgagatcggc tcctcggggg cgcccacgtt gaccagcgtg
     1861 ccgtcggtgc gcagcagagc gaggtaggcg ccgaggtcga gcggggccga gaccgtggag
     1921 aggatgacgt cgaacgtgcc ggccagctcc tcgaaggtcc gcggatcgct ggtcgcgtag
     1981 tagtggtccg cgcccagctt gagcccgtcg tccttcttcc gcagcgactg ggagaggacc
     2041 gtgacctcgg caccgagcgc gtgcgcgatt ttgacggcca tgtggccgag tccgccgaga
     2101 ccgacgacgg cgaccttctt gccggggccc gcgccccagc gcttgagcgg ggagtacgtg
     2161 gtgatgcccg cgcacaggag cggcgcggcc tcgtccaggg cgatgccctc ggggatggag
     2221 agggcgaacg tctcggccac gacgatgtgc gtcgagtagc cgccgtacgt gggctcgcca
     2281 ccgcggtcga gagcgttgta cgtctgggtg ttcccctcgg cgcagtgctg ctcgcggccc
     2341 agcaggcagg cgtcgcacgt tccgcaggag tcgaccatgc agccgacgcc gacgcggtcg
     2401 ccgaccttga accgggtgac cccggggccg gtctcggcga cgatcccggc gatctcgtgg
     2461 ccgggcacca tcgggaagat gccctcgccc cagccgtcgc gggcctggtg gatgtcggag
     2521 tggcagatcc cggcgaactt gatgtcgatc aggacgtcga actcgcccac cgggcggcgt
     2581 tcgatcgtgg tgcgctccag cggggccttg gcacggggag cggcgtaggc ggcaacggtg
     2641 gtcatgccgg aggttctcct ctgggtgatg cggtaggaac gtctcccagc atcacgccgt
     2701 gacggatgtc tacccaggtc accgctttgc ctacgtccag tggtcctact actgacgggg
     2761 acaggctcgg gctcgtacga cctgggatca caggaataat ggaaaccatg gacgatcgac
     2821 cggagggagc cgcccccgag ccgctggacg ggcgcgccga gctcagcgag ttcctgcgca
     2881 cccgcagggc caggctccag ccgggcgacg tgggactccc ggagttcggc cggcaccgcc
     2941 gggtgcccgg tctgcgccgg gaggagctgg cacaactggc cggggtctcc gtggcgtact
     3001 acacgcgcct ggagcaggga aacgggcgca acgtgtcggc cgaggtcctt gacgcgatcg
     3061 ctcgggccct gcggctcacg gacgccgaga gcgcccatct gacgcacctg gccaagcccg
     3121 cgcggcacag gaagaagcgc cgcccggcca gggtgcagcg cgtgcgcaag ggcctgctgt
     3181 acttgctcga cagcatggac ggcatccccg cgtacgtgac cggggcacgc tccgacgtcc
     3241 tcgcctggaa tccgatggcg gcggcggtgt tcggcgactg gggcgcgctg ccgccgggcg
     3301 agcgcaactg ggcgcggctg gtgttcctct cccccgacta ccgggacctg ttcgcgaact
     3361 gggactccaa ggcctcggac atggtcagct atctgcggct gtacgcgggc tgccaccccg
     3421 acgatccgga gctctcggcg ctggtgggcg aactctccgt caagagcgac gagttccggc
     3481 ggctgtgggc cacgcacaac gtcaaggaga agggccacgg cagcaaggtg ctgcggcatc
     3541 cgctcgtcgg ggagctgacc ctgtcgtacg agacgctgaa cctcccggac gacgaggagc
     3601 agcatctggt gacctaccac gcggagccgg gttcggagtc gtaccaggcc ctgcgtctgc
     3661 tggcgagctg gggagcggac gcgggccggc ccggcgctgt cagcgaggcg ggccgaggtc
     3721 cggccggcga gcgatgagga cgtgctccct gccggggcgg ccgggcgcct cgcgcacgtc
     3781 acgtacgacg aacccctgtg ccgccaggtc cgcctcggcc ccctgacgtc cgcggaagca
     3841 cagggtggag tcggaggtga ggacctgcct gcctggccgt cgctccggaa cacgtaggtc
     3901 cagcggaccc cggtctggtc cgagtgggtc gtggagtgca gccggccggg gatgcgcgaa
     3961 gatccgtctt tccggcttct tttcggagaa cggaggaatc acggcagaga agagtgagcg
     4021 ctttccgtgc acgcctctcc cgtgaatccg cataacggac cggaaaggaa atgcgcgagt
     4081 cgtcgctcct gtgcgcggtc ggcgagtgtg cggggcctcc cctcgccggt gatgcggcct
     4141 tattccggcg tgaacgcctc cgcgtgctcg gccgaccaga cgcggtacgg cctcgccggg
     4201 cggcccgtca gttcctggac cgtgggcacc acggctgcct tcgctcccgc ccgctgccgc
     4261 tccgcgctcc gcaggaaggc ctccgcgacg ggcggcggat acttcgccag cagggccgtg
     4321 cgcgcggtgt ccaggtccag ttcctcgaag cgcaacggcc gccccagcac ccgggagagc
     4381 tgggccgtct gctcccgcgc ggtgatcgcc tccgggcccg acacggcgta cgccctgccc
     4441 tcgtgccccg taccggtgag gacggcgacg gccaccgcgg cgacgtcgcg cgggtcgacg
     4501 cacgcgaccg gagcgtcacc gtacagggcg cgcaccactc ccgccgaccg gatgccgggc
     4561 ccccaggaaa gggtgttgga catgaacgtc ctggggcgca cgaacgtcca cgggaccccc
     4621 gactccctca ccgcctgttc gttctcccgc tgacgccggg tgatgaagtc gtcggcgccc
     4681 ggctcctcca ccgccatcat ggacagcttg accaggtggc gtacgcccgc cgcggccgcc
     4741 gccgccgcga ccctctcgtc gtcgggctcc gtggggctgt tcgtcaccag gaacaccgag
     4801 gcgactcccc gcagggcccg gtcgagagcg gcgcggtccc cgtactcgcc ctcgacgatt
     4861 tcgacccctc ccccctgtac ggtgagccgc tcggggcggc gcgcgagaat ccgcacgggg
     4921 ccggccgccg ccagctgtcc ggcgacctca cggcccaccg ctccggtcgc acccgtcacg
     4981 agaatcactc gggaaacctc tcagccgtgg gcccgggggc cggacaggac ggtggagaac
     5041 accgtttcgc cgtcctgatg gcctgtcacc tgtaccgtca ccgtatcctc ggccgtcccc
     5101 ggcaggacca ccgccctgat ccagcacgga ctgccgaact cggagtaccg gtggaagcgg
     5161 gtgcgcgcct cggccggaac gatccccgcg ggcccggccg cgaggcacgc cgcctggcgg
     5221 gccgcctcca gcagcaacat gcccgggaca tggtcattgg ggcgctggaa cagggtggga
     5281 tgccgggtgt ccacgcgcag ttcccagact ccgtcccgcc cggtctccga gagcaccacg
     5341 tcctcgacgc gggtacggcc cgcccggacg gccgggaccg gcacggtctc cggtatggat
     5401 atcccctcgg ccggggcgtc gccgcgcatc cgcctgtacg ccttgggagt gctgaaacgg
     5461 gtctcccccc ttcccgtcgc ggcgagccga ccgtcgcggt acaccgccca gccgacacgc
     5521 ccctgggccg ggagcccgcc ccgccacttc aggtcggagc agaacacctc cacgtcgatc
     5581 tcggtgggtg cgccccccac ggccaggtgc tcctgatgac agacgtagtc gagtcccgtc
     5641 atcaggaact gatagccgag cgggatcccg tacccggcgt ggaaggcgag catggccgcc
     5701 tgccgcatcg cctccgcgac cagcagcggg tcgtgccggt cgtcacccac cggagcgaag
     5761 aacggatgat cgtgcggaag gaccgccccc accgagaacc ggtcgtgctc caggcggacc
     5821 cagttccgcg gaaacgcgtc ctccggcctg gtccggtgca ccatgtcgat ccccacggag
     5881 tgcaccacct cggcctccgc atccatggtc ggcaccgaat cgatcaaaac tgctgcttcg
     5941 ggcataactc cccccagggg tcatgtagtg gcgagccgtt cctgcgatgc ttatatcggt
     6001 ataataagac gagaatactc ggtctttttc cctttacccg ggacgaatcc cccacgtccg
     6061 ccccgccgca tttccgggcc ccgcccggga atgcggatga cggcggcgcg ggcattgagg
     6121 aggcgcgtcg tcccggccgc gaagtccgcc ggaattcgcc ggagcgccga ctccgcgaaa
     6181 tgtgccggcc gctccaattt tccgcgcgcc cagcggcgcc cggcccggga cgccgggctc
     6241 ggacggatgc accccgtcca gggcgagaac gacggcccgt tgcacgcttc ccatggcacg
     6301 atcctgtcga aagatggcga tcatggcatg gtacgccggg ggtgcgccct cgaccctgga
     6361 cgtgtcgagt gcccggggag gaccctgggc actggcgaag gccgaacgct caccctcgcg
     6421 gcgttccggc gttccggcgt tccggcgttc aggcgttcag gcgttcaggc gttcaggcgt
     6481 tcaggcgttc agcgccagct tcgcgccgag cccgatcatc atcacggcta ccagaccgtc
     6541 cagcacccgc cacgcggagg gccgcgcgag gaccccgctc agcagccggg cgccgaaccc
     6601 gagcgcggcg aaccagcaca cgctggcgag gatcgcgccg aggccgaagg tccagcgcaa
     6661 cggtcctcgg tcggctgcga cggatccgag caggaacacg gtgtcgaggt acacgtgcgg
     6721 attgagccag gtcatcgcca ggcaggtcag caccacgctc ccgcgcgagg cggccggggc
     6781 gtcccccgcg tgcagggcgg acgggcgcag gacgcggcgt gcggcgagca gtccgtagcc
     6841 gatcaggaac acgccgccga gcagtccgac cacggtcagc gccgaaggcc aggccacgac
     6901 gagggcgccg acccccgcga cccccagcgc gatgagcaca gcgtcggaga gggcgcagat
     6961 cccgaccacc gcgagcacgc cgtcgcggcg gacgccctgg cgcagcacca gggcgttctg
     7021 ggctccgatg gcgacgatga gcgagaggcc ggtgccgaaa ccggcggccg cggtggtcag
     7081 ggcgttcgtc atgcgggcga cggtacggag cggggaaacc tggtgtccag ctaaagattc
     7141 ttacgtatca tcagtgttca tgatgtccga gcttcctctc gaccaggtgc ggaccctgct
     7201 ggccgtggtc gacgaaggca ccttcgactc cgcggcgaac gccctgcggc tgacgccgtc
     7261 ggcggtcagc cagcgggtga aggcgctgga gcagcgcacg ggccgcgtgc tgctgatgcg
     7321 cacgaagccg gtgcggccca ccgagtccgg tgaggtggtg gtgcgcttcg cccgccagct
     7381 ggccagactg gagcaggacg cgcaggcgga gctcgggatg tccgggccgg gcgaaccgac
     7441 gctcctgccg atcgcggtga actccgattc cctggcgacc tggttcctgc ccgctctgcg
     7501 acgcgtgccc gaggagctgg ggctctgtta cgagctgcgc cgggaggacc aggaccatac
     7561 ggccgccctg ttgcgggagg ggctcgcgat ggcggcggtg acctcgtcgc cggaggcggt
     7621 gacgggctgc tcggtacgcc cgctgggccg gatgcggtat ctgccggtgg cgagccccgg
     7681 tttcgccgac cggtggctgg ggcggcggga gggcaccgcg ctgcgtgaac tgatcggcga
     7741 cgccccggtg atcggcttcg accggcgcga cgaccttcag gacgctttcg tgcgccggct
     7801 cggccccggc cgccggccga gcccccggcg ccacctggtc cccacgtcgg aggggttcgc
     7861 gcacgccgtg gcgtcgggca tgggctgggg catggtgccc gaggtccagg cggagccgtt
     7921 gctgagcggc ggacggctcg tgcggctcac cccggaccac accgtcgacg tcccgctgta
     7981 ctggcagcag tggaaactgg agtccccggc cctggcggcc gtcgccgagg cggtggtggc
     8041 ggaggccgcc gaggcactcg acggcgactg gccggactga ccgccgcacc gcccggtcac
     8101 ggggcggtgc ggccacgcac gattcgctca tgatcggccg tccatgatgg ggggcaggtt
     8161 ccgaagtcca cgcacgggag tcacgtatgc cccagcccgc cgtccgtccg ttcctccgcc
     8221 gtgggagcac cctcgccggt ctggccgtcg gcgtcgcgac cgcgacggta gctgggctgc
     8281 tcctgtccga cacgttcgcc tcgggcacca cggccgatgc cgcgcccgag gtcgtgccgc
     8341 ccgaggcgaa cgtgaccttc gactaccaga tcggcggcgg gtattccccg cccgccgggg
     8401 tcggggcggt ctcccgcgac cgtcgcgacg agccggccga gggcctgtac aacgtctgct
     8461 acgtcaacgc cttccagacc cagcccgacg cgctggacca gtgggagaag aaccacccgg
     8521 atctgctgct gcgcgacggc gacggccggc tggtcaacga cgaggactgg ggcgaggcgc
     8581 tgctcgacac ttcgacggcg gacaagcgcg cccggctggc ggatatcgtc gggggctggg
     8641 tcgacggctg cgcgaagtcg ggcttccagg cggtggagcc cgacaacctg gattccttcg
     8701 agcgctcaga aggattgctg acccgggagc acaacgcggc gttcgcggag ctcctggccc
     8761 accgggcgca cgccgccggg ctggcgatcg gccagaagaa caccaccggt ctgctggagc
     8821 ggcgggcgga gatcggcttc gacttcgccg tcgccgagga gtgcggccgg tacgacgagt
     8881 gtgccgacta cgcctccgcg tacggcgaca gggtgttcgt cgtcgagtac accgacggcg
     8941 acttcgcgaa ggcgtgttcg tccgtgggga cgacggtctc ggtggtgcgc cgggacctcg
     9001 acgtccggcc ggccggacag ccggggtacg tgttccgcac ctgttgaacg gctctttcgg
     9061 ggacgggtgg ctcagggggc gcccgtcggg tgggcgcaga tggtgaagat gccgccgtcg
     9121 gggtccctga tcaccgcttc ggtcccgtcg gcgggcgagt ccaccggcgg tccacccgtg
     9181 ccgcccgcgg ctcgggccgc ctcggtggcc gcttccaggt ccgcgaccgg gaagcggacg
     9241 tgccagcgcg ggcgcagccg ggggtcgggg gaggacgcgt cgccgcctcc gcgcagggtg
     9301 gccacggtgc ggccgccctg ccgtacgacg accgtgtcct gctggtacgc gtagccgacc
     9361 tcgcagccgc cgggttcccc ggaggcccag ccgaacacct cggcatagaa gatcgcgcag
     9421 tcgaaggcgt ggctggtccg cagttcgatg gaggcgggag ggtggtctcg gccgaacgac
     9481 cagggcgggg tcctgccttc ccagaagccg aatccggcgc cttcccggtc ggcggccacc
     9541 gctccccgtc cggggcccac ccggatcggg ccgacggcga tggtgccacc ccgttcacgg
     9601 atccgggccg ctgtggcgtc gacgtcggcg acggcgaagt acggtgtcca gctgacttcc
     9661 gtgggcagac cgtgggcgat ctgcccgatc ccggcgaccg gcagggcgtc ggcgtgggcg
     9721 acggtgaagc cctcgccgag ggtgccggag cggaaggtcc acccgagaac ggtcccgtag
     9781 aagtccttgg tggcccgcag gtcccgggtc atgagactga cccagcacgg aaccccgtgc
     9841 accggggtgc tctgctctgc cgcggtcatc gggatccgtc tcctcgtccc cgggtggcga
     9901 gccgccggac gtggacgccg gtccggcggg tgggcctgtc ggtcacctgc cgtcgcaggc
     9961 cggtgcgctc cgtggcgtcg cacgtcctcc ggagcggcac gcctccatac ctacgccgcc
    10021 ggggtcgccg ccgccacccg ggccgcgccc cggaacggtg cggagcgcac gcccccggcc
    10081 ggcccggacg ggaccccgca ggtcagtggc ccgggacagg ctgcccggtc ggccgcctcg
    10141 cagccgaggg cggcctcccg cacagttgcc gggcgaccgc gcggggtggg agaagtgccg
    10201 gcagcgcccg gagccggggg cgtgggaggc cggactgccg ttccgccggg tttcgtcgcc
    10261 cgttcagtgt ccggcgagcg ccgcgggttc gccgagccgg tgcgcggcgg tgcgccaggc
    10321 ggcggtgacg gcttcgcggg ccgtgacggt cgccgcctcc acctccggcg ggagctgcag
    10381 cggggcgccg acgtggacgt ggagccgggg gcggcgggcc ggagcggtga ggatcccgga
    10441 gatctgcttg acgcacgaac ccgaggtcac gcggcgggct cccgcctggc cgagcggcac
    10501 gacgggcgcg ccggacgcgt gcgcgagacg ggcgaggccg ctgcggaagt tttccggcgg
    10561 ggcctccgcc gcgtccgtgc ggcggggcag ccgtccttcg ccgtagatga gcagatgacg
    10621 cccgtccctg agcgcggccg ccgccgtgtc gagggcgtcc gcggcccggt cggtgttccg
    10681 gtgcacgggc acgtgcccgc cacgctccag gagacggccg agtaccggta cgcgccacag
    10741 gcccgcggtg gccatgacga cgggttcgac gccgaggcgc agcagtgcgg cgaggacgat
    10801 gcccgggtcg gccagggagg tgtggttggc gacgatgatg ctgccggcgg ccggagccgt
    10861 ggcgtggtcg gatgtgaccg tgagtcgtcc gagcgagggg acgacggcgg cggcgaggcg
    10921 gctgagcacg gttctcccgg ggtcc
//
