LOCUS       NZ_CP094446            10384 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Paenibacillus thiaminolyticus strain Mbale2 chromosome, complete
            genome.
ACCESSION   NZ_CP094446
VERSION     NZ_CP094446.1
KEYWORDS    RefSeq.
SOURCE      Paenibacillus thiaminolyticus
  ORGANISM  Paenibacillus thiaminolyticus
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 6487160)
  AUTHORS   Hehnly,C., Shi,A., Ssentongo,P., Zhang,L., Isaacs,A., Morton,S.U.,
            Streck,N., Erdmann-Gilmore,P., Tolstoy,I., Townsend,R.R.,
            Limbrick,D.D., Paulson,J.N., Ericson,J.E., Galperin,M.Y.,
            Schiff,S.J. and Broach,J.R.
  TITLE     Type IV Pili Are a Critical Virulence Factor in Clinical Isolates of
            Paenibacillus thiaminolyticus
  JOURNAL   mBio 13 (6), e0268822 (2022)
   PUBMED   36374038
REFERENCE   2  (bases 1 to 6487160)
  AUTHORS   Zhang,L. and Hehnly,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2022) Institute for Personalized Medicine,
            Pennsylvania State University College of Medicine, 500 University
            Dr, Hershey, PA 17033, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 05-APR-2021
            Assembly Method :: Canu v. 08/2018; Pilon v. 08/2018
            Assembly Name                     :: PSU_P.thiaminolyticus_Mbale2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028554415.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 07:15:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,889
            CDSs (total)                      :: 5,780
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 109
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 201
            CDSs (without protein)            :: 201
            Pseudo Genes (ambiguous residues) :: 0 of 201
            Pseudo Genes (frameshifted)       :: 146 of 201
            Pseudo Genes (incomplete)         :: 68 of 201
            Pseudo Genes (internal stop)      :: 17 of 201
            Pseudo Genes (multiple problems)  :: 26 of 201
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:53:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5561407
            Orig. end                         :: 5571791
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP094446.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Bacteria and source DNA
            available from Schiff/Broach Lab, 500 University Drive Hershey, PA
            17033.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10384
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="17"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10384
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10384
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5384](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5384
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            865..1527
                     /gene="ehuC"
                     /locus_tag="L3476_RS25170"
                     /old_locus_tag="L3476_25110"
     CDS             865..1527
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuC"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679974.1"
                     /locus_tag="L3476_RS25170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_25110"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="WP_143802206.1"
                     /transl_table=11
                     /translation="MKSASAILEYVPALLQGAWVTLQIVCFSMMLAFICAFIAGLARLS
                     KYRIIRFVSAVYVEVFRGTSLLVQLFWLYFALPMLLDIRMSAMAAAVLALGLNYGAYGS
                     EVVRGAILSVPKGQYEAAVALNMSPWLRMRRIVLPQAMALMLPSFGNLLIELLKGTSLV
                     YFITLMDLTYQGMVLRSFDNSSTPQIFALLLLIYFIMAYSLTLGIRWVERRAVKGRV"
     gene            1531..2181
                     /gene="ehuD"
                     /locus_tag="L3476_RS25175"
                     /old_locus_tag="L3476_25115"
     CDS             1531..2181
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuD"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679975.1"
                     /locus_tag="L3476_RS25175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_25115"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="WP_272826484.1"
                     /transl_table=11
                     /translation="MWSWDYALEILPQLLQVLPITIGATLLGFAAACMLGLPLALARRS
                     RYRAVSVLANAWMEFIRSTPLLVQLFVLFYALPLYGISFSPFVTGVIGLGLHYSAYLSE
                     VFRSGIEAVSAGQWEAAKALNFGRRHSWTNIILPQAIPPIIPVMGNYLITMFKETPVLS
                     AITLVELLQTAKAIGSGSFRYLEAFTMVGLLFLLLSYPSSLLVSRLEKRFQNK"
     gene            2198..3064
                     /gene="ehuA"
                     /locus_tag="L3476_RS25180"
                     /old_locus_tag="L3476_25120"
     CDS             2198..3064
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679976.1"
                     /locus_tag="L3476_RS25180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_25120"
                     /product="ectoine/hydroxyectoine ABC transporter
                     ATP-binding protein EhuA"
                     /protein_id="WP_143802204.1"
                     /transl_table=11
                     /translation="MESVQCRPLASQAAHDPIVMYRNICKSYGSVKVLQDINLSIYPGE
                     KVAVIGPSGSGKTTLARILMTLERPTSGTIQVDGEWLWHQEVKGKLVAAKEKHLHNVRS
                     KIGMVFQHFHLFPHMTILRNVTEAPVHVLGWSKQEAEARAMEMLAKVGLEDKVKEYPAR
                     LSGGQKQRVAIARAVVMRPKVMLFDEATSALDPELVGEVLTVIKDLAAEGDMAMMLITH
                     EMDFAREIADRILFTDGGSIVEQGTPEQIFENPQSPRLQAFLSRFRQTWYMGGVQQASP
                     SAEEGGR"
     gene            3061..3606
                     /gene="ectA"
                     /locus_tag="L3476_RS25185"
                     /old_locus_tag="L3476_25125"
     CDS             3061..3606
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679977.1"
                     /locus_tag="L3476_RS25185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_25125"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_272826486.1"
                     /transl_table=11
                     /translation="MSMDVNVTQSLPCTIRNARKGDGTDIWKLVTESGTLDVNSAYCYI
                     MLCEYFADSCIVAEHNRNIIGFVSALRPPEMPDTVFVWQIAVRAGYRGRGIAEALLRTL
                     YEAACRDRVRFMETTIAPSNKTSQRLFAKMAKTWKSALVTKEGFSSQLFPEGAHESEKL
                     IRIGPLRHNIHDRMETVT"
     gene            3603..4886
                     /gene="ectB"
                     /locus_tag="L3476_RS25190"
                     /old_locus_tag="L3476_25130"
     CDS             3603..4886
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (31-366). E-value: 2e-78.
                     Score: 255.7. Matches aSDomain:
                     nrpspksdomains_L3476_RS25190_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679978.1"
                     /locus_tag="L3476_RS25190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_25130"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_272826487.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.7e-75, bitscore:
                     252.7, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVLDGRTTSLQVFESLESEVRSYCRSFPTVFTKAQGYKLWDADN
                     REYIDFFAGAGALNYGHNNRTMKRKLLDYIQQDGVTHSLDMATKAKEQFLKKFHRVILE
                     PRGLAYKVMFPGPTGANTVESALKLARKVTGRSTVFSFTNAFHGMTLGALSVTGNKFKR
                     QGAGIPLHHTVTMPYDGYLGSDIDTLDYMECYMNDSGSGVSRPAAIIVETIQGEGGINE
                     ASNDWLQRLERVCRSHEIVLIVDDVQMGCGRTGTFFSFERAGITPDIVCLSKSISGYGL
                     PMALTLIRPDLDIWEPGEHNGTFRGNNLAFITAAEALDYWNDERFTQAIMAKGKMIRSC
                     LEELVAQYPELQGTARGRGLMQGIAFGIPGMAERLCARAFAKGLIMETSGTDSEVAKIM
                     PPLTIDDTGLLAGLDILRTCMKEMAEEL"
     aSDomain        3696..4700
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_L3476_RS25190_Aminotran_3.1"
                     /evalue="2.00E-78"
                     /label="L3476_RS25190_Aminotran_3.1"
                     /locus_tag="L3476_RS25190"
                     /protein_end="366"
                     /protein_start="31"
                     /score="255.7"
                     /tool="antismash"
                     /translation="FTKAQGYKLWDADNREYIDFFAGAGALNYGHNNRTMKRKLLDYIQ
                     QDGVTHSLDMATKAKEQFLKKFHRVILEPRGLAYKVMFPGPTGANTVESALKLARKVTG
                     RSTVFSFTNAFHGMTLGALSVTGNKFKRQGAGIPLHHTVTMPYDGYLGSDIDTLDYMEC
                     YMNDSGSGVSRPAAIIVETIQGEGGINEASNDWLQRLERVCRSHEIVLIVDDVQMGCGR
                     TGTFFSFERAGITPDIVCLSKSISGYGLPMALTLIRPDLDIWEPGEHNGTFRGNNLAFI
                     TAAEALDYWNDERFTQAIMAKGKMIRSCLEELVAQYPELQGTARGRGLMQGIAF"
     gene            5001..5384
                     /locus_tag="L3476_RS25195"
                     /old_locus_tag="L3476_25135"
     CDS             5001..5384
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679979.1"
                     /locus_tag="L3476_RS25195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_25135"
                     /product="ectoine synthase"
                     /protein_id="WP_143802202.1"
                     /sec_met_domain="ectoine_synt (E-value: 3.3e-51, bitscore:
                     170.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVKQLSDILGTEHDVKAETWNSRRLLLKKDGVGFSLHDTLIKAG
                     TETEIWYKHHVEAVYCIEGEGEIEVVESGMVYPIQPGTLYVLDGHEKHLLRGKSQMRML
                     CVFNPPCTGHEVHDAEGTYPLEV"
     gene            5467..6378
                     /gene="thpD"
                     /locus_tag="L3476_RS25200"
                     /old_locus_tag="L3476_25140"
     CDS             5467..6378
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679980.1"
                     /locus_tag="L3476_RS25200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_25140"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_143802201.1"
                     /sec_met_domain="PhyH (E-value: 8.3e-37, bitscore: 125.9,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIILRGQSQAQEAARTPDMYPSRVNTETLLLKRPDPVVYNHTIRQ
                     TEITADQLASYEADGYLFLDSFFSEDEVQVWKRELLRLFQDHKDSAKPEVIREPGSDEI
                     RSIFAVHREGLFRQLANHPRILPIVQQILGSQVYVHQSRINFKQGFTGKEFYWHSDFET
                     WHVEDGMPRMRALSCSIALEENNANNGPLMVIPGSHRMFVSCIGATPDDHYKSSLRRQE
                     YGIPDQESLIYLANQGGIVAPTGKAGSILLFDCNVMHGSNSNITPFPRSNVFIVYNSVE
                     NPLEPPYSGKPPRPDFIAARSS"
     gene            complement(6870..7448)
                     /locus_tag="L3476_RS25205"
                     /old_locus_tag="L3476_25145"
     CDS             complement(6870..7448)
                     /GO_function="GO:0016209 - antioxidant activity [Evidence
                     IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /locus_tag="L3476_RS25205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_25145"
                     /product="redoxin domain-containing protein"
                     /protein_id="WP_143802199.1"
                     /transl_table=11
                     /translation="MTDFIIIITLVLVLIQLLLIFMLARLIGQFLAKIKSVGGISLTEL
                     QIGEQVPLFREWGSQGRKVILRELLSHRKVLLLFISTTCQTCKSILPDLQRVVENWDIH
                     IVVINTDKQSNDEEIEVMLPSGIDYIRSVDIMDAYGVSKVPYVFLVNQNGILESHHSLM
                     SANMLWNMLENETARSHDKVPSPLSVMNE"
     gene            complement(7473..7997)
                     /locus_tag="L3476_RS25210"
                     /old_locus_tag="L3476_25150"
     CDS             complement(7473..7997)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /locus_tag="L3476_RS25210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_25150"
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="WP_272826488.1"
                     /transl_table=11
                     /translation="MFQNVLAFLFLSTFLHKTLNYREHIRIMNAYHLVPARLIVPSFIL
                     FAAAELAIGLTLLLSGITLYHVVSIGSLLSIYTFAIGFNLMRGHRHIDCGCGGILENEQ
                     LSWGLVLRNLLLGFSVAGIWKSQSLYIGEIALIHQLELVMLSLILFSLLYLFKELFNIR
                     KKLVRLMKMLI"
     gene            complement(8015..9844)
                     /locus_tag="L3476_RS25215"
                     /old_locus_tag="L3476_25155"
     CDS             complement(8015..9844)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="L3476_RS25215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_25155"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_272826489.1"
                     /transl_table=11
                     /translation="MMANIDERRGIMYSFSNIWRGSGFAILYIWRLNKPYFYVSITLHL
                     VLGLLPLLSVWTMQELVNEVILIAQPGHGFATALSILSFQMVIILSAYILQFLSQLNDQ
                     KLENLLGLVLKKQIFRKIMNLPYSTFEDPSFYDQNQRIINSHFQIVSMVKAIMSLGSTI
                     ITVVSLVGYITHIHWGLLVIIFLFSIPILMIHIRFGNKRYALTRFLTPYHRREMYISDL
                     LSQRDSLKEIRLFGLGSHLIEKWSSFYRRSAQEKYSLSKNQGIWELAGSMLLTLTYIVS
                     GILIILLISAKRIEIGAFVAALQSIQNIQSSLTEISASLSNIYEISLYLEDFKSFQQKK
                     ELTSSSSQIKCGAINQISVQNLTFTYPNQIEPTLKNIHLSIKKGQKIAIIGENGSGKTS
                     LIKCILGLYDTGENTICVNQIPLHNYDIASYHQRIAALFQDFIEYEFSARENIGFGNLN
                     NPSKPEVIAAAKKTKIHEHISKLPEHYESLLGRHFEGGHELSGGQWQKLALSRALFKNS
                     DVIILDEPTSSMDPESELDIIHELFQGNDDKAALIITHRLGVAVQADYILVMKEGRIVE
                     EGTHQELLRQQGEYHRLYRAQTKIYNQESGVFV"
ORIGIN
        1 ccattatgct ggtaagctgt gtgccgaaga gcggcggcag ccagaccgaa gcgggcggcg
       61 ggacatcgcc ccaggagacg gcgctggaca aagcgaggcg ggaaggatac gttaccgtgg
      121 gcttcgcgaa tgaaaagccg tatgcttatg ccacgccgga tggccaactg acgggcgagg
      181 ccgtggaggt agcccgggaa gtgttgaagc ggatgggcat cgaggagatg aacggcgtcc
      241 tgacggaatt cggttctctc attccgggat tgaaggcgaa gcgcttcgac atcatcacgg
      301 caggaatgtt catcaatccg gagcggagca aggaagtagc ctttgccaac cccgaataca
      361 gcattggcga ggcgctcgcg gtcaagaagg ggaatccgtc caatctgcgc agctacgagg
      421 atattgctgg caacaaggat gtcactgtcg ccgtcatggt gggtgccatc gagctcgaat
      481 atttagcgaa atcaggtgtc gcgaaggaac aaatcgtaac ggtgccggat caaacgtcgg
      541 ccatctcggc tttgcaggcg gggcgcgtgg atgcggtgac gatgacggga ccgtccttgc
      601 aggcgatgct tgactcgacg aaggacgatc aattggagcg agtcatggac ttccagcagc
      661 cggagataga cggcacaagc gtaagagggt atggagcggc ggcgttccgc aaggaagata
      721 ccgccttccg ggaagcattc aatcaagagc tggaagcgtt gaagcaatcg ggcgagctgc
      781 ttgaactatt gaggccgttc gggttcacgg agcaggagct tcctggagat atgacggctg
      841 aacggctgtc gcaagagtga agccatgaag agcgcttccg ctatacttga atatgtgcct
      901 gcgctgctgc aaggagcgtg ggtcacgctg caaattgtat gcttctccat gatgctcgct
      961 ttcatctgcg cttttatagc gggattggct cgtctgtcca agtaccgcat cattcgcttt
     1021 gtcagcgcgg tttacgtaga ggtctttcga ggcacttctt tgctggttca gttgttctgg
     1081 ttatattttg cgctgcccat gctgctcgac atccgcatgt cggcgatggc tgcggccgtg
     1141 ctcgcgcttg gtttgaatta tggcgcttat ggatctgaag tggtgcgggg ggcgatcctg
     1201 tccgtgccca aggggcagta cgaagcggcc gttgcgctca atatgagtcc gtggctgcgg
     1261 atgcgaagaa tcgtgttgcc gcaagcgatg gcgctcatgc tgccttcgtt cggcaatctg
     1321 ctcatcgagc tgctgaaggg aacatcgctt gtgtacttca tcaccttgat ggatttgacc
     1381 taccaaggca tggtattacg ctcctttgac aactccagta caccgcagat ttttgcattg
     1441 ctgctcctga tttatttcat catggcctac tccttgacgc tcggcattcg atgggtggag
     1501 cgcagagccg tgaaggggag ggtgtagcgt atgtggagtt gggactacgc attggagatt
     1561 ctccctcagc tgctgcaggt gcttccgatt acgatcggag cgacactgct cggatttgcg
     1621 gcggcatgta tgcttgggtt gccattggcg cttgctcgcc gatcccgata tcgagccgta
     1681 tctgtgcttg cgaatgcgtg gatggaattc atccgcagca ctccgctgct cgttcagttg
     1741 ttcgtactct tttacgcatt accgctttac ggcatctcat tctctccgtt tgtcaccgga
     1801 gtcatcggat tgggcttgca ttatagcgct tatctatccg aagtatttcg ctcggggatt
     1861 gaggcggtgt ccgccggtca atgggaagcg gccaaggcgc ttaacttcgg caggcggcat
     1921 tcatggacga acatcatcct tccccaggcg ataccgccaa tcattcccgt catgggcaac
     1981 tatttgatta cgatgttcaa ggaaacgccc gtcttgtcgg cgatcacgct tgtcgaactg
     2041 ctgcagacgg ccaaagcgat aggttccggc tcattccgct atctggaagc ctttacgatg
     2101 gtcgggttgt tatttctgct gcttagctat ccttcctctc tgcttgtcag cagattggag
     2161 aagagatttc agaacaaata gggggtggag agcgaagatg gagagcgtcc aatgcaggcc
     2221 gctggcctcg caagcggcac acgatccaat cgtcatgtat cggaatatat gcaagtcgta
     2281 cggctctgtg aaggtgctgc aagatatcaa tctatccatt tatccggggg aaaaggttgc
     2341 cgtcatcggc ccgagcggat ccggcaagac gacgttggcc cgcattctca tgaccttgga
     2401 gcggcccacc tcgggaacga ttcaagtcga cggggagtgg ctatggcacc aagaagtgaa
     2461 gggcaagctt gtggcggcga aggaaaaaca tctgcacaac gtacgcagca agatcggcat
     2521 ggtctttcag cactttcacc tttttccgca tatgaccatt ttgcgcaatg tgacggaagc
     2581 gccggtgcac gtgttgggat ggagcaagca ggaagccgaa gcacgcgcta tggagatgct
     2641 ggctaaggtt ggactggaag acaaggtgaa ggaataccct gcacgcttgt ccggcgggca
     2701 gaagcagcgc gtggccattg ccagagccgt agtcatgcgt ccgaaggtga tgctcttcga
     2761 cgaagcgacc tccgcgctcg atccggagct ggtcggcgag gtgcttacgg tcatcaagga
     2821 cttggccgcc gaaggcgata tggcgatgat gctcatcacg catgaaatgg attttgccag
     2881 ggagattgcc gatcggatcc tgtttacaga tggcggctcc atcgttgaac agggaacgcc
     2941 ggagcaaata ttcgagaacc cgcaaagccc gcgcctgcaa gcctttttga gccggttcag
     3001 gcagacgtgg tatatgggag gagttcagca agcttcacca tctgcagaag aggggggacg
     3061 atgagcatgg atgtcaacgt cacgcaatcc ttgccctgca cgatccgcaa cgcgcggaag
     3121 ggcgatggaa cggacatatg gaagctggtg acagagagcg gcacgctcga tgtcaactcg
     3181 gcctattgtt atatcatgct gtgcgaatat ttcgccgaca gttgcatcgt ggctgagcac
     3241 aatcggaata ttatcggctt cgtatccgcg cttcggccgc cggagatgcc ggacacggta
     3301 ttcgtctggc aaatcgcggt gcgtgcgggc tatcgaggcc gagggatcgc cgaagcgctg
     3361 ctgcgcacat tgtacgaggc ggcatgccgg gatcgggtgc gattcatgga gacgacgatc
     3421 gcgccaagca acaagacatc ccagcgcctg ttcgccaaga tggcgaagac atggaagagc
     3481 gcgctcgtga cgaaggaggg gttctcctct cagctatttc cggaaggagc gcatgagagc
     3541 gagaagctga tccgcatagg gccattgcgc cacaatatac acgatagaat ggagacggtg
     3601 acatgaaggt attggatgga agaacaacat cgttgcaagt attcgaatcg ttggagtccg
     3661 aggttcgaag ctactgcagg tcgttcccga ccgtatttac gaaggcccaa ggctataagc
     3721 tgtgggatgc ggacaatcga gagtatatcg atttcttcgc cggagccggc gcgctcaact
     3781 acgggcataa caatcggacg atgaagagga agctgctgga ctatattcag caagacggcg
     3841 tgacgcatag cctggacatg gccacgaagg cgaaggaaca atttctgaag aaattccatc
     3901 gggtcatctt ggagccgcga ggattggctt acaaggtgat gttccccggg ccgacaggcg
     3961 cgaacacggt ggagagcgcc ttgaagctgg cgcgcaaggt aacggggaga tcaactgtct
     4021 tcagcttcac gaacgccttt cacggcatga cgctgggcgc cttatccgtc acgggcaaca
     4081 agttcaagcg gcaaggggcg ggcataccgc ttcatcacac ggtcacgatg ccgtatgacg
     4141 ggtatctggg atcggatatc gatacgctcg attatatgga atgctatatg aatgacagcg
     4201 gcagcggagt gtcgcggccg gccgccatca tcgtcgagac cattcagggt gaaggcggga
     4261 ttaacgaggc gagcaatgat tggctgcagc ggctggagcg ggtatgccgc agccacgaga
     4321 ttgtgctcat cgtggacgat gtgcagatgg gatgcggccg cacagggacc ttcttcagct
     4381 tcgagcgggc gggcattacg ccggacatcg tctgtctgtc caaatcgatc agcggatatg
     4441 gactgccgat ggcgcttacg ttaatccgcc ccgatctgga tatttgggag cctggcgagc
     4501 acaacggcac gttcaggggc aataatttgg cattcatcac cgcggctgag gcactcgatt
     4561 attggaacga tgaacgcttt acgcaagcaa ttatggccaa aggaaagatg attcgctctt
     4621 gcttggaaga gctagtcgca caatatcctg aactacaagg aacggcgaga ggccggggac
     4681 tgatgcaggg gattgccttc ggcatcccgg gcatggcgga gcggctctgc gccagagcct
     4741 tcgcgaaggg gctcatcatg gagacatcgg gtacagacag cgaagtggcg aagattatgc
     4801 cgccgctcac gatcgatgac acgggtctgc ttgcaggctt ggatattttg cgaacatgca
     4861 tgaaggaaat ggcggaagag ctgtagcaag ccgtcatatc ggcgcacggt gaaatgttgg
     4921 agaacatgat attgttgaga acacgatatt atggggaacg cgaaattaag gagaacacga
     4981 acaaatagga ggaatctacg atgatcgtca agcaattgtc tgatattctc ggcacggaac
     5041 atgatgtgaa ggcagagaca tggaacagcc gcagattgct gctaaagaag gacggagtcg
     5101 gtttttcctt gcatgatacg ctgatcaagg caggcacgga gaccgaaatc tggtacaaac
     5161 atcatgtgga ggcagtctac tgcatcgaag gagaagggga aatcgaggtc gtcgagagcg
     5221 gaatggtcta tccgattcaa ccggggacgt tatatgtgct cgatggtcat gagaagcatc
     5281 tgctgagagg gaaaagccag atgcgcatgc tgtgcgtatt caacccgcct tgcacgggcc
     5341 atgaggtgca tgatgcggaa ggcacatatc cgttagaagt ctagtcgcgg gtgcatgcgg
     5401 acgaagacta tgacgactga cataaaagct ggaacgcgaa ccgccatact ttttcacgga
     5461 aggagcatga tcatattgag aggacaaagc caagcgcagg aggctgcaag aacgccggat
     5521 atgtatccgt ccagagtgaa tacggagacc ttattgttaa agcgcccgga tccggttgtc
     5581 tacaaccata cgatcaggca gacggagata acagccgatc agcttgcttc ttacgaagcg
     5641 gacggatact tgttcctcga ttcgttcttc agcgaggatg aagtgcaggt atggaagcgg
     5701 gagctgctcc gcttgttcca ggatcataag gacagtgcga agccggaagt gattcgggag
     5761 ccgggcagcg acgagatacg ctcgattttt gccgtgcatc gggagggtct attccggcag
     5821 ctggcgaacc acccgagaat cctgccgatc gtgcagcaga ttctcggcag tcaagtctat
     5881 gtgcatcaat cgcgcattaa tttcaagcaa ggctttacag gcaaagagtt ctactggcat
     5941 tccgacttcg agacatggca tgtagaagac ggcatgccgc gcatgagggc attgagctgc
     6001 tccattgcgc tggaagagaa caatgcgaac aacggtccgt taatggtcat ccccggatcg
     6061 caccgcatgt tcgtctcttg tatcggggcc acgccggacg atcattataa gtcatcgctg
     6121 cgccgccagg aatacggcat tcccgatcag gagagtctca tctatctggc caatcaaggc
     6181 ggcattgtgg cgcctacggg caaggccggc tccattctat tgttcgactg caatgtgatg
     6241 catggctcga acagcaatat tacgccgttc ccgcgcagca atgtattcat cgtctataac
     6301 agtgtcgaga atccgcttga accgccgtac tcaggcaagc cgccaagacc tgactttatt
     6361 gcggcccgtt catcctagca gaagacggtt atcaataggg agaagaccca tcgcggatgg
     6421 gtcttctttt accgaacgtc cagcattggg tgcccccata cttgataata aaaccaaata
     6481 tggattttat aatataaatg gtatataatg tgtattaatg tgtatgcaat aaaaatatta
     6541 gtaatggagg taatgagagt gaaaaatttc ttgagcaagt tgacaggatt ccagcctgct
     6601 gccgcacgct gcatcttaac gcgcagagtt gtctcctgcc gctccgacgc cctgtgcatt
     6661 atgcaaaaag tgtcctcctg ggagtactat gttgatcgtg atacgggaga agagtgtaca
     6721 aggccaacaa aagtaagatg cggctgctag gaacaagcct attttcgcca gattcgccag
     6781 aataaaatgg atgaaccagg gaagcgattt ggcctctaac aagatgcaaa atcgcttctt
     6841 tctgtagcca tttgtctgac tgcgcaaacc tactcattca taaccgataa ggggcttggg
     6901 accttgtcat gggaacgtgc cgtttcgttc tctaacatat tccacagcat gttcgcactc
     6961 attaacgaat gatgggattc caggattccg ttttggttca ctagaaaaac atagggaact
     7021 ttgcttaccc cataagcgtc cataatgtcc acagaacgaa tataatcgat gccggaaggg
     7081 agcatcacct cgatctcttc gtcattggat tgcttatccg tattgataac gacgatatgt
     7141 atatcccaat tttcaaccac tctttgtaag tcaggcaata tgcttttgca ggtttggcat
     7201 gtcgtactga taaagagaag cagcactttt ctgtggctca acaattccct tagaatcact
     7261 ttgcgcccct gtgagcccca ctcccgaaaa agcggaactt gctctccgat ttgaagctcg
     7321 gttaacgaaa tcccgcccac gctttttatt ttcgcaagaa attgaccaat gagccttgcc
     7381 agcatgaata taagcagcaa ctggatgagc accagcacta aagtgatgat aatgataaaa
     7441 tcggtcattc gaatctcctt cctcgtttgt tttcagataa gcatcttcat aaggcgaaca
     7501 agtttttttc ggatgttgaa taattccttg aacagataga gcaacgaaaa aaggatgagc
     7561 gagagcatga ccagttcaag ttgatggata agcgcaatct ctcctatgta caatgattga
     7621 gatttccata tacctgcaac cgaaaaaccg agaagaagat tacgaagcac taatccccag
     7681 ctcagctgtt cattttcaag aatcccgcca catccgcaat ctatatgccg atgccctcgc
     7741 atcaaattga aaccgattgc aaaggtataa atacttagca gtgaacctat ggacaccaca
     7801 tgatacagcg tgatcccgga caagagaagc gtgaggccta tcgcaagctc ggccgcggca
     7861 aataatataa aagacggaac tatgagtcgg gcgggcacaa ggtgataagc attcatgatg
     7921 cgaatatgtt cacggtagtt cagcgtttta tgtaaaaagg tggagagaaa caaaaaggca
     7981 agcacatttt ggaacataac aacgagaccc atgtttagac gaaaaccccg ctttcctggt
     8041 tataaatttt ggtttgcgcg cgatataaac gatggtattc tccctgttgg cgcaaaagct
     8101 cctgatgtgt gccttcctcg acgatgcggc cttccttcat caccaaaata tagtccgcct
     8161 gcacggcgac gccaagacga tgcgtaatga tcagcgccgc tttgtcatcg ttcccttgaa
     8221 ataattcatg aataatatcg agttccgatt ccgggtccat ggatgaggta ggctcgtcaa
     8281 gaatgattac atcgctattt ttgaatagag ctctggataa ggccagcttt tgccattgcc
     8341 ccccggataa ttcatggcct ccttcaaaat gacgtcccag cagtgactca taatgctcgg
     8401 gcagcttgga tatgtgctca tgaattttcg tttttttggc ggcagcaatc acttcgggct
     8461 tactcgggtt gttcagattg ccaaagccga tattctcccg ggcgctgaat tcatattcaa
     8521 tgaaatcctg aaataaagcg gcaatccgtt gatggtagga tgcgatatca tagttgtgta
     8581 gaggaatttg gttgacgcat atcgtatttt ctcccgtatc atacagacct agtatgcatt
     8641 tgatcagcga ggtcttgccg gatccgtttt ctccaatgat ggctatcttt tgtccttttt
     8701 taatagacag atgaatattt ttgagggtcg gttctatttg atttggataa gtaaacgtga
     8761 gattttgcac gctgatctgg ttgatggcgc cgcatttgat ttgagaagac gaacttgtca
     8821 gctccttttt ttgttgaaaa gatttgaaat cctcgagata caaactgatt tcatagatat
     8881 tggatagtga agcggaaatt tcggttaagc tggactgaat attttgaatc gattgcaggg
     8941 ccgccacgaa cgcccctatt tctatcctct tggccgagat cagcaggata atgagaatac
     9001 cggacacaat ataggtcaac gtgagcagca tgctgccggc aagctcccat atgccttgat
     9061 ttttggataa actgtatttt tcttgcgcgc tccgcctata gaatgaagac catttttcga
     9121 tcagatgcga gcccagtcca aagaggcgaa tttctttcaa ggaatctcgt tggcttagga
     9181 gatcggatat gtacatttct cttctatggt agggagtcag aaatcgagtg agcgcatatc
     9241 ttttattgcc aaaacggata tgaatcatca ggatcggaat cgaaaataga aagataatga
     9301 ccagcaatcc ccaatggatg tgggtaatgt agcccaccaa cgacacaacc gtgataatgg
     9361 tgcttcctaa agacatgatg gccttaacca tcgacacgat ttggaagtgg ctgttgataa
     9421 ttcgctggtt ctgatcataa aaagacgggt cctcaaaggt ggagtaaggc agattcataa
     9481 ttttccggaa tatttgtttt ttgagcacca gtccgagcag attttcaagt ttttgatcat
     9541 ttaattgaga taaaaattga agaatatagg cagacagaat gatgaccatt tggaagctta
     9601 aaatcgataa tgctgtggca aatccatgcc cgggttgagc gattaaaata acctcgttca
     9661 cgagttcttg catcgtccat acgctaagca gaggcaataa acctagcacg agatgaagcg
     9721 taatggacac gtaaaaatag ggtttgttca atctccaaat ataaagaatg gcaaacccgg
     9781 aaccgcgcca aatatttgaa aaagaataca ttattcctcg cctttcatca atatttgcca
     9841 tcatagtaaa agacaggtat gatataaact gagtatatac ataatatggc aggtgcgcaa
     9901 ggacgagatt tttatgtcgg aggttgaatg tggctatcta ttttcaattc gctttgctag
     9961 cgtgcgttat attcgcaact gtcgtagtct tcagattctt gtcctctatg cttcagcgga
    10021 cgaacagcat gggggggaga aggggagagc cgccgcctat accccttact gcaccagcaa
    10081 gctccctcat ttgtcgtgcc ggatgccggt gatacccgtt ttgatttaag ccatgccata
    10141 gcgaagaatg gaatcctgct catatttatc gattcacact gcccttattg catcccatca
    10201 ttagagagat ggcttcaagc aagcatggag cggggaacgg ctctacatgc tgcagttttg
    10261 ctaaaagaaa gcggaattga cggctacaaa gaactgtata gccatagtaa tggaatccga
    10321 tttttgcaag ccggcgagaa catatttgaa tcctatcaga ttacggaata tccgtacttt
    10381 gtcc
//
