LOCUS       NZ_CP094446            23890 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Paenibacillus thiaminolyticus strain Mbale2 chromosome, complete
            genome.
ACCESSION   NZ_CP094446
VERSION     NZ_CP094446.1
KEYWORDS    RefSeq.
SOURCE      Paenibacillus thiaminolyticus
  ORGANISM  Paenibacillus thiaminolyticus
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 6487160)
  AUTHORS   Hehnly,C., Shi,A., Ssentongo,P., Zhang,L., Isaacs,A., Morton,S.U.,
            Streck,N., Erdmann-Gilmore,P., Tolstoy,I., Townsend,R.R.,
            Limbrick,D.D., Paulson,J.N., Ericson,J.E., Galperin,M.Y.,
            Schiff,S.J. and Broach,J.R.
  TITLE     Type IV Pili Are a Critical Virulence Factor in Clinical Isolates of
            Paenibacillus thiaminolyticus
  JOURNAL   mBio 13 (6), e0268822 (2022)
   PUBMED   36374038
REFERENCE   2  (bases 1 to 6487160)
  AUTHORS   Zhang,L. and Hehnly,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2022) Institute for Personalized Medicine,
            Pennsylvania State University College of Medicine, 500 University
            Dr, Hershey, PA 17033, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 05-APR-2021
            Assembly Method :: Canu v. 08/2018; Pilon v. 08/2018
            Assembly Name                     :: PSU_P.thiaminolyticus_Mbale2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028554415.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 07:15:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,889
            CDSs (total)                      :: 5,780
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 109
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 201
            CDSs (without protein)            :: 201
            Pseudo Genes (ambiguous residues) :: 0 of 201
            Pseudo Genes (frameshifted)       :: 146 of 201
            Pseudo Genes (incomplete)         :: 68 of 201
            Pseudo Genes (internal stop)      :: 17 of 201
            Pseudo Genes (multiple problems)  :: 26 of 201
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:53:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4267914
            Orig. end                         :: 4291804
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP094446.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Bacteria and source DNA
            available from Schiff/Broach Lab, 500 University Drive Hershey, PA
            17033.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..23890
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lassopeptide"
                     /region_number="13"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..23890
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..23890
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:13890](+)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..13890
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     gene            complement(663..1154)
                     /locus_tag="L3476_RS19295"
                     /old_locus_tag="L3476_19275"
     CDS             complement(663..1154)
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /GO_function="GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006678386.1"
                     /locus_tag="L3476_RS19295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_19275"
                     /product="GAF domain-containing protein"
                     /protein_id="WP_272825950.1"
                     /transl_table=11
                     /translation="MFTPSQYSGQPDQDYPLLHKQLLALIEGEPNLIANLANASALLWQ
                     GLKDINWAGFYLMDESKEELVLGPFQGLPACIRIPLGRGVCGIAAQERRTLVVPDVHAF
                     PGHIACDAASRSEIVVPMIKDGRLVGVLDIDSPLTDRFQEPDREALEKMVEALMKEAVL
                     "
     gene            1999..2928
                     /locus_tag="L3476_RS19300"
                     /old_locus_tag="L3476_19280"
     CDS             1999..2928
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (34-288). E-value:
                     2.4e-15. Score: 48.7. Matches aSDomain:
                     nrpspksdomains_L3476_RS19300_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006678387.1"
                     /locus_tag="L3476_RS19300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_19280"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_272825951.1"
                     /sec_met_domain="PF00561 (E-value: 2.2e-11, bitscore: 42.3,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 4.5e-11, bitscore:
                     42.2, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLSNTFTFTDPEGVRVHVYRWDPEEGQPVLGVIQIAHGMTETARR
                     YSRFAEALTDAGYVVYGNDHRGHGRTAERPEDLGYVGEDGFTWMVRNMAQLTEIIQSEQ
                     AGLPVFLFAHSMGSFLAQKYIAEHGDKINGVILCGSNGPRGRELYAGVALTKLIASARG
                     SRHRSKMIDNMAFGGFNRTFRPSRTSFDWLSRDEQEVDKYVADPECGFLSTIGFYRDLF
                     RLLQDIHRPETMQAIPKELPVMLIAGDRDPVGQYGKGVRRLAEMYRELGIREVECILYP
                     EARHELLNEKNRDEVTSDCLSWLRNHTP"
     misc_feature    complement(2036..2047)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 19.75"
                     /tool="antismash"
     aSDomain        2101..2862
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_L3476_RS19300_Abhydrolase_1.1"
                     /evalue="2.40E-15"
                     /label="L3476_RS19300_Abhydrolase_1.1"
                     /locus_tag="L3476_RS19300"
                     /protein_end="288"
                     /protein_start="34"
                     /score="48.7"
                     /tool="antismash"
                     /translation="IAHGMTETARRYSRFAEALTDAGYVVYGNDHRGHGRTAERPEDLG
                     YVGEDGFTWMVRNMAQLTEIIQSEQAGLPVFLFAHSMGSFLAQKYIAEHGDKINGVILC
                     GSNGPRGRELYAGVALTKLIASARGSRHRSKMIDNMAFGGFNRTFRPSRTSFDWLSRDE
                     QEVDKYVADPECGFLSTIGFYRDLFRLLQDIHRPETMQAIPKELPVMLIAGDRDPVGQY
                     GKGVRRLAEMYRELGIREVECILYPEARHELL"
     gene            3048..4058
                     /gene="namA"
                     /locus_tag="L3476_RS19305"
                     /old_locus_tag="L3476_19285"
     CDS             3048..4058
                     /EC_number="1.6.99.1"
                     /GO_function="GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA]"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /gene="namA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019419299.1"
                     /locus_tag="L3476_RS19305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_19285"
                     /product="NADPH dehydrogenase NamA"
                     /protein_id="WP_272825952.1"
                     /transl_table=11
                     /translation="MPQLFEPLTLRGVTIPNRIVMSPMCMYSCHNEDGMVNDWHLVHYT
                     SRAVGQVGLIMIEATAVTPGGRISAQDLGIWSDEHVDGLRRLTDMVHAHGRKIGIQLAH
                     AGRKATVPGIIAPSALRFSEAYETPEEMTLEDIRKTIKAFADAARRAEEAGFDVIEIHG
                     AHGYLINQFLSPLTNQRTDSYGGGADARYRFLSEVIEAVRITWSKPLFVRISANEYAEG
                     GNAIEAYIDYARRMKDQGVDLIDCSSGGVVPHRVEAYPGYQVQYAHAIRQSAGIATGAV
                     GLITEPSLAEEIVRNGRADFIFLGRELLRDPYWPRQAAKQLRQELAPPKQYDRGW"
     misc_feature    complement(4302..4319)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 18.69"
                     /tool="antismash"
     regulatory      4318..4593
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /regulatory_class="riboswitch"
     misc_feature    4515..4534
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: medium, score: 24.66"
                     /tool="antismash"
     gene            4822..7174
                     /locus_tag="L3476_RS19310"
                     /old_locus_tag="L3476_19290"
                     /pseudo=""
     CDS             4822..7174
                     /GO_function="GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006678391.1"
                     /locus_tag="L3476_RS19310"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_19290"
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /pseudo=""
                     /transl_table=11
                     /translation="MLTTNHTVQTVHKPNNRSLAFDAERIMRYGERIMGAYPELDRERW
                     HRGVLAKLRHSHVQASDITQACIMTALELVSVEEPKWKFVAARALLTKLYKEAALHRSY
                     KAYADRPYGDFYKLIKQLADIGIYRQDLLEAYTPDQIRELGQAIDPACDELFDYIGLLT
                     LAERYLAVDFEGRTMELPQERYMIIAMYLMHQEPEERRVELAKEAYWAMSNLYMTAATP
                     TMANAGKKVAGQLSSCFIDTVDDSLEGIFDSNTDVARLSKMGGGIGVYLGKVRARGSDI
                     RGHKNTSSGVIPWIRQLNNTAVSVDQLGTRKGAIAVYLDVFHKDILSFLDLKLNNGDER
                     MRAHDIFHGVCIPDLFMEAVEKREDWHLFCPHEVKKTMGWTDGNGRPLGLEDFYDEQAG
                     QGQFREKYAEAVATPHLSRITLPAIDIMKRILKSQFETGTPYMFYKDTANRANPNKAHG
                     MIFCSNLCTEIMQNMSPTTIEQEELVLEDGRARIIISKLPGDFVVCNLNSIHLARAVRA
                     DVLERLVPIQVRMLDNVIDINNIEVLQAQHTNRRYRAIGLGTFGLHHLLALEGIAWESD
                     EAVEYNDYLYERIHYLAVRASMDLAKEKGAYPLFAQSEWENGRYFEQREYTTGTRPGGF
                     VTTEQWNELAAEVKANGMRNAWLMAVAPNGSTSIIAGSTASIDPVYELLSYEEKTTYKI
                     ANPAPDLSDKTMWYYKTAFRLDQNWSIRMAGSPPAPYRPRPELQPVRPSGYPGRRFP"
     misc_feature    7219..7232
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 20.08"
                     /tool="antismash"
     gene            7275..8306
                     /locus_tag="L3476_RS19315"
                     /old_locus_tag="L3476_19295"
     CDS             7275..8306
                     /EC_number="1.17.4.1"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013308516.1"
                     /locus_tag="L3476_RS19315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_19295"
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="WP_206108719.1"
                     /transl_table=11
                     /translation="MKLQKIFNTEAPNQSTRIILGECSGILNWDDIRMPHMYKLYKVLL
                     LNHWIADEIPMSKDAQQFPNLTPDEQRAFKINISLLAVLDSMQTMFVGDVKRFFTDSSL
                     EAIAAIIAQQEVVHNQSYSYVLSSLVSEQEQKDIFEYWKHDAVLLERNRFIADIYQTFR
                     DNPTPQTFFEALVADLILEGVFFYSTFAFFYNLARDQKMMATSQMISYIQRDENQHCYF
                     FGEVFKQLLRDFPELNTDANRRFVHDTIDRAVQLETNWAHYTLSNIRGIDLRELSDYIK
                     YIANRRLAMMGLEKAYEGVDVNCMPWIKPFSDEALNSTKTDFFEAKSRNYGKVGDDNGF
                     DEL"
     gene            complement(8733..9695)
                     /locus_tag="L3476_RS19320"
                     /old_locus_tag="L3476_19300"
     CDS             complement(8733..9695)
                     /EC_number="2.7.8.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_4"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006678395.1"
                     /locus_tag="L3476_RS19320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_19300"
                     /product="MraY family glycosyltransferase"
                     /protein_id="WP_143798063.1"
                     /sec_met_domain="Glycos_transf_4 (E-value: 1.6e-39,
                     bitscore: 133.9, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFYYVFAFLLSVCIVLLLIPPFKKLAFRIGFVDQPQKDNERKIHR
                     QPIPLLAGAAIFISFCVTYVLFVPHSLTKMAGILGGGLLILSIGMVDDWYKTRRKDFPA
                     LPKLIVQLGAAALVYASGIAFIGFNNPFTGQFIYLPEWLQFLLTVTWIFGVTTVINFSD
                     GMDGLAGGLTAISAGTLFIVGISHGQGTPAMMALILVGAALGYLRYNKPPANVFMGDAG
                     ATFLGFMLGVVALDGAFKQATLLSLFIPILAVGVPIFDNILVVIRRMLNGQPIYKADAT
                     QAHYRLLAVGLNQKQVVIFLCLLNLCFGLTSIILSLLPV"
     gene            10001..11950
                     /locus_tag="L3476_RS19325"
                     /old_locus_tag="L3476_19305"
     CDS             10001..11950
                     /GO_function="GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA]"
                     /GO_process="GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GATase_7"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006678396.1"
                     /locus_tag="L3476_RS19325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_19305"
                     /product="asparagine synthase-related protein"
                     /protein_id="WP_272828819.1"
                     /sec_met_domain="GATase_7 (E-value: 6.9e-18, bitscore:
                     63.2, seeds: 307, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 8e-26, bitscore:
                     89.9, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGAVAGIYHLQHDKLEPGLGRQMIDALRRFPADYVSSWSNGSLFL
                     GSLQQCITPQSINEVLPCYDPVTSLAITSDAIIDNRDELADQLQLSKPARQTITDSQLI
                     LQAYMKWGHDMPKHLVGDFAFIIWDGRDRTMFGARDFSGTRTLYFHQSPELFSFCTIME
                     PLLTLPHVKRDLNEHWISQFLAIPITTDAVDSFSTVYQSIKQIPPSHSIFVANGKVIFQ
                     QYCRLQHTEKLVLTSNEEYEEAFRDVFQTAVQARLRTFRQVGAHLSGGLDSGSVVSFAA
                     PMLQANNKPLYTFSYYPIEGFSDFTSWSRFADEREYIRSTAEHVGNIEMNVSSYPSSNP
                     YSVIDEWLDTLEMPYKFFENSFWLKGIYEQASARDIGVMLTGQRGNWTISWGPAMDYQA
                     NLLRKGRLVSFFWEHRRYCQSLGANPWKVLQIVGKKSFPRLAPLIAPGGDPALPVLINP
                     DFARKTNVTDHMKACGMDVWGSSTQNAYDIRKDHFNQLYFWNINGTIGTKLSLKHKIWD
                     RDPTNDLRVVRFCLSVPDEQFVQQGVDRSLIRRATARYLPDKVRLNRKRRGVQGADGVF
                     RLMPDWSGLIRELEQMIGDPRTSYYLNAPLLRELLNEIRHRPRPELAFDLKFRVLMRGF
                     IFHKFLSRISGKEV"
     gene            11953..12090
                     /locus_tag="L3476_RS19330"
                     /old_locus_tag="L3476_19310"
     CDS             11953..12090
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002172970.1"
                     /locus_tag="L3476_RS19330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_19310"
                     /product="paeninodin family lasso peptide"
                     /protein_id="WP_115057746.1"
                     /transl_table=11
                     /translation="MKKQYSKPSLEVLDVHQTMAGPGLSTPDAFQPDPDEDVHYDPMDS
                     "
     CDS_motif       11953..12012
                     /prepeptide="leader"
                     /note="peptide class: lassopeptide"
                     /note="predicted sequence: MKKQYSKPSLEVLDVHQTMA"
                     /locus_tag="L3476_RS19330_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       12013..12090
                     /prepeptide="core"
                     /locus_tag="L3476_RS19330_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class II"
                     /score="-9.90"
                     /molecular_weight="2684.7"
                     /monoisotopic_mass="2683.1"
                     /core_sequence="GPGLSTPDAFQPDPDEDVHYDPMDS"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="MKKQYSKPSLEVLDVHQTMA"
                     /leader_location="[11952:12012](+)"
     misc_feature    12163..12177
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 23.29"
                     /tool="antismash"
     gene            12195..13136
                     /locus_tag="L3476_RS19335"
                     /old_locus_tag="L3476_19315"
     CDS             12195..13136
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="L3476_RS19335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="L3476_19315"
                     /product="aldolase"
                     /protein_id="WP_272825953.1"
                     /transl_table=11
                     /translation="MTERAAVRTDHYKAFGFRIESDFVLPELPLAGEREPLDNITVRRT
                     DLQPLWNSSIHFYGNFAILDHGRTVMFRVPGAAIYAVQDASSILVSPFDQAEENWVRLF
                     ILGTCMGILLLQRKIMPLHGSAVAIDGKAYAIIGASGAGKSTLALHLVSEGYPLLSDDV
                     IPVVMTQGSPWVVPSYPQQKLWVDTLKHMGMDNANYTPLYERKTKFAVPVGSNFHEEPL
                     PLASIFELVPSDAATHIAPIQGMERFRVLFHHTYRNLLVQPLGLMEWHFKTLSSFIRQV
                     GMYRLHRPMVGFSTLDLTSHIFNITRQGENDQ"
     gene            13133..13432
                     /locus_tag="L3476_RS19340"
                     /old_locus_tag="L3476_19320"
     CDS             13133..13432
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006678398.1"
                     /locus_tag="L3476_RS19340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_19320"
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_119790116.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 8.5e-23,
                     bitscore: 78.6, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 2.9e-13, bitscore: 48.3,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKLHSITPVDTLVQCEGHIVSDMAGEKVMLSVQKGKYYNLGTLG
                     GEIWDMLITPVKAEHIIRSILSEYEVESSECEEDILLFLSDLEHEGLIRHVKRL"
     aSDomain        13181..13414
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_L3476_RS19340_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="4.10E-25"
                     /identifier="RREFam002.1"
                     /label="L3476_RS19340"
                     /locus_tag="L3476_RS19340"
                     /protein_end="94"
                     /protein_start="16"
                     /score="78.6"
                     /tool="antismash"
                     /translation="EGHIVSDMAGEKVMLSVQKGKYYNLGTLGGEIWDMLITPVKAEHI
                     IRSILSEYEVESSECEEDILLFLSDLEHEGLIR"
     gene            13429..13890
                     /locus_tag="L3476_RS19345"
                     /old_locus_tag="L3476_19325"
     CDS             13429..13890
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002160861.1"
                     /locus_tag="L3476_RS19345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_19325"
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_272825955.1"
                     /sec_met_domain="PF13471 (E-value: 1.3e-24, bitscore: 84.9,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNRLRRWIWLCLSDRKTFLLFAEAFLYLGWARILLYFPFARISPL
                     LGRRSVETPEVHDPSQTKTMKHIRSAIQIASRHTFWESKCLVRAIAGMKMLEKRNLHST
                     LYLGTGKNKNGELIAHAWLRSGSIYITGAEMMKQFVVVEKFAKSAGTDP"
     gene            13887..15710
                     /locus_tag="L3476_RS19350"
                     /old_locus_tag="L3476_19330"
     CDS             13887..15710
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006678400.1"
                     /locus_tag="L3476_RS19350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_19330"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_272825956.1"
                     /transl_table=11
                     /translation="MNSIILYMKRLHKTAGLQLSINIIGMVISSLLEGMTFLLLIPMLT
                     VGGVLLSEPAWENRLRFLNILHSLPQASSLAIILTVYLLIVVTQNLIHRAVTVRNAEIE
                     QHFSRQLRYENYSALLRAQWSFFINRRTSDLVNALTIETARVLGGISLLLQFITSALFT
                     LLQIIFAFWISPKITLFVIACGAMLAFLSRGFIRKSKQLGSQTSKLAQAYLAGITDQLQ
                     GMKDIKSNNLEPSRLEWHQQLTEGTMQEQMDYVRLRLNSQLLYKIASSLLVAGFIFTSF
                     RFFPSEGPSLLIVILLFARLWPRFTSLQSKLEQLASTIPAFKALQQLQRDCKLAADQAI
                     TLTESPAPGPGPWFVMQGIEVRNLSFRYQPQLPVYALQNISVHIPAQGMTAIVGKSGAG
                     KSTFIDILMGLMQPESGQILVDGIAITNDNLLAYRRSIGYVAQDPFLYNATIRENLLMV
                     KPSATENELWEALDFSSAAEFVRKLPRQLETLIGDRGIRLSGGERQRLVLARAIIRQPS
                     ILVLDEATSALDTENEQKILTALHRLKDRITVIVVAHRLSTIQNAEQILVIHDGKLVEQ
                     GTFLSLSSEKKSIFANLLQNQEAQPVNVYTL"
     gene            15778..16113
                     /locus_tag="L3476_RS19355"
                     /old_locus_tag="L3476_19335"
     CDS             15778..16113
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1148: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006678401.1"
                     /locus_tag="L3476_RS19355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_19335"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_272825957.1"
                     /transl_table=11
                     /translation="MIGERIQILRRKKNYSLSELSERAGVAKSYLSSIERGIQQNPSIQ
                     FLEKISAILGVSVEELLQDKEASNEAEALDEEWTNLVKEAMASGVSKEQFKEFLEYNKW
                     KLTREES"
     gene            complement(16236..16406)
                     /locus_tag="L3476_RS19360"
                     /old_locus_tag="L3476_19340"
     CDS             complement(16236..16406)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639518.1"
                     /locus_tag="L3476_RS19360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_19340"
                     /product="anti-repressor SinI family protein"
                     /protein_id="WP_115057747.1"
                     /transl_table=11
                     /translation="MVEAVTGNNEQLDKDWVAIILTARQMGLSIADIRKLLRDFQAQSD
                     PEWPLHVNSEA"
     regulatory      complement(16424..16509)
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /regulatory_class="riboswitch"
     gene            complement(16599..17627)
                     /gene="galE"
                     /locus_tag="L3476_RS19365"
                     /old_locus_tag="L3476_19345"
     CDS             complement(16599..17627)
                     /EC_number="5.1.3.2"
                     /GO_function="GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (70-200). E-value:
                     4.5e-08. Score: 24.4. Matches aSDomain:
                     nrpspksdomains_L3476_RS19365_NAD_binding_4.1"
                     /codon_start=1
                     /gene="galE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006678403.1"
                     /locus_tag="L3476_RS19365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_19345"
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="WP_272825958.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 6.9e-13, bitscore:
                     46.7, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 9.2e-15,
                     bitscore: 52.9, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAILITGGAGYIGSHTCVEMLEAGYDIVVLDNFSNSHPEAIRRIS
                     DITGKGFAFYEADLLDGQRLNRIFEGHRIDAVIHFAGLKAVGESVQVPLEYYHTNLTST
                     LVLCRAMAKHGVYNLVFSSSASVYGVPECVPISEEASLGAVSPYGRTKQMIEQVLQDLA
                     SSDPRWRISILRYFNPVGAHKSGKIGESPNGTPNNLMPFITQVAVGRLPSLKVYGSDYP
                     TKDGTGIRDYIHVVDLAQGHLKALEHLDHTNGAEFYNLGTGRGFTVLEMIRSFEKVSGI
                     QIPYEIVGRRPGDIGICYADPTKSHISLGWFPKYGITDMCEDSWNWQVHNPQGYCERIT
                     AG"
     aSDomain        complement(17028..17417)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_L3476_RS19365_NAD_binding_4.1"
                     /evalue="4.50E-08"
                     /label="L3476_RS19365_NAD_binding_4.1"
                     /locus_tag="L3476_RS19365"
                     /protein_end="200"
                     /protein_start="70"
                     /score="24.4"
                     /tool="antismash"
                     /translation="GHRIDAVIHFAGLKAVGESVQVPLEYYHTNLTSTLVLCRAMAKHG
                     VYNLVFSSSASVYGVPECVPISEEASLGAVSPYGRTKQMIEQVLQDLASSDPRWRISIL
                     RYFNPVGAHKSGKIGESPNGTPNNLM"
     CDS_motif       complement(17259..17315)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L3476_RS19365_0002"
                     /evalue="8.80E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="L3476_RS19365"
                     /protein_end="123"
                     /protein_start="104"
                     /score="8.8"
                     /tool="antismash"
                     /translation="LVLCRAMAKHGVYNLVFSS"
     CDS_motif       complement(17556..17621)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L3476_RS19365_0001"
                     /evalue="8.90E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="L3476_RS19365"
                     /protein_end="24"
                     /protein_start="2"
                     /score="15.2"
                     /tool="antismash"
                     /translation="ILITGGAGYIGSHTCVEMLEAG"
     gene            complement(17627..19219)
                     /locus_tag="L3476_RS19370"
                     /old_locus_tag="L3476_19350"
     CDS             complement(17627..19219)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222557.1"
                     /locus_tag="L3476_RS19370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_19350"
                     /product="sugar translocase"
                     /protein_id="WP_272825959.1"
                     /sec_met_domain="Polysacc_synt (E-value: 1.2e-09, bitscore:
                     36.5, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKPGKRAGTGMRIRNSLYNIFFGLLGQAISTIMGFIVRTVFIWTL
                     GVEYLGVSGLFSDVLMLLSLANLGFETAIIYSLYQPLADRDEARIKAFMHLFRKAYLFI
                     GMLVLVLGLSLIPFLDVFIQGETTVSHIEVIYVLFLLNSVSTYFFAYKQAIIVADQQQH
                     VISRIHSVYVIAMNTVQIIVLLCTKNYILFLVIQIGMSILRNKYISRVADRKYEFLREP
                     VSTPLDKGEKKTFFQNLRSLLMYKVSGVVINGTDNLIIANLFGVIWVGLYSNYFLILTT
                     LTTFLSYFFYSLTASVGNLNATEDKEKKFFFYRVLRLANFWVFGLSAVCLWNLLDPVII
                     LWLGGSFVLGKETLLAILLNFYTTGMQNASTMFRDTTGMFRRGRYSPLIAAAMNIGLSI
                     TLARFIGMPGVFLGTVISRLLTYFWVDPYLLHKHVFLQPLRPYFTRYALHAALVLLAAS
                     LCGWLAGLLPLPLLWDIAVRVLLCLSVTNLIFYVCFRKSSEFIYLMNIAKANLGKLPIL
                     TKFNPVIRREETR"
     gene            complement(19206..20471)
                     /locus_tag="L3476_RS19375"
                     /old_locus_tag="L3476_19355"
     CDS             complement(19206..20471)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006678405.1"
                     /locus_tag="L3476_RS19375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_19355"
                     /product="peptidase"
                     /protein_id="WP_143798058.1"
                     /transl_table=11
                     /translation="MIQLTREQIKVNQNRVDYFFQVDPRLQKYFRASNHLFMKYNYDIS
                     GLPASILSIPFAANMMPLAWLTDATLYVDELDRSFYDCLAELKQAYQEMFPHFPLKGQI
                     KVGQVSTYHYQPEHEAAVLFSGGLDALTTYIRHRDKKPLLITEYGWHEGDCTDSEVWTA
                     DREHAEAFARKEGLSNILVESNYGTFVYPQRIDRDFRRSLGDTWWHGLHHGLAIISAAV
                     PAACLLQVKTIYIASSHTIGNTYSCASDPSTDNLIRYGAGDVFHDGYELSRQDKVKVVV
                     DHYAAANDKTAVRVCYRNTENCCQCEKCVRTIMGIAAEGHDPNDYGFPVPDELSGFLKR
                     FLKREVKFFTNIQIERLWKPIQERMRENPDNVKDQDLLSWFPDYDFVAHRRKERFRYRV
                     TQFFPIIRRKVRTGLQQIRETR"
     gene            complement(20496..21467)
                     /locus_tag="L3476_RS19380"
                     /old_locus_tag="L3476_19360"
     CDS             complement(20496..21467)
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006678406.1"
                     /locus_tag="L3476_RS19380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_19360"
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="WP_143798057.1"
                     /transl_table=11
                     /translation="MDNILLLDTSVGSLNKGDEIIMKCVRRQLSGITSQANVFTVPTHL
                     SPFGWFQVMRDSLRVQFYTRAKYKFVGGTNLLTMDMFTHFPQWNINVFNYRPLQGSILL
                     GVGAGKGEKIKRYTKMLYRKVLSHEYVHSVRDERTWRFVTGLGLKALNTGCPTMWALTP
                     EHCQDIPKGKSDAVVFTLTDYARAPRQDQLLIDLLIRSYRKVYFWVQGADDYAYFHTLS
                     NTDSIHVIPPSLEEYEAVLSTDVDFVGTRLHAGIYAMRHKKRSIIIAVDERARGMSETY
                     ALNTVDRTDLAGLEAMIHSEFATNVRMNLEAIQAWLDQFKQR"
     gene            complement(21479..22681)
                     /locus_tag="L3476_RS19385"
                     /old_locus_tag="L3476_19365"
     CDS             complement(21479..22681)
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006678407.1"
                     /locus_tag="L3476_RS19385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_19365"
                     /product="glycosyltransferase"
                     /protein_id="WP_143798056.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 3e-24, bitscore:
                     84.0, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRVLFASFDMEVGGVERSLAGLLEHFDYERYQAELMLYRHQGDF
                     MPLLPEQVRLLEEQRAYATFRKSIGDIIKSRQLLIGLGRLTAKVHADMAGKARGCAETG
                     YHQMQLMWRYTLPFLPRNKQLYDAAVSFLWPHYYVAEKVDARTKIAWIHTDYSTVATNV
                     RSDLKLWSKFDHIIAVSPSCREAFLAKYESLRDKVTVIENIISPDYIKMMSEVSVESPM
                     AADPRFKLVTVARLSHAKGIDQAVMALKKLREKGLEGIAWYVVGYGGDEEMIRGLIAKN
                     RLQEDFILLGKQYNPYPFIKQADLYVQPSRYEGKAVTVTEAQILGKPVLITRYPTAQSQ
                     VADGLDGYITDLSVEGLAEGIEHLYRDRQLRCRLADMCSRTQYGNAGELNKLYALLDDN
                     G"
     gene            complement(22678..23862)
                     /locus_tag="L3476_RS19390"
                     /old_locus_tag="L3476_19370"
     CDS             complement(22678..23862)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_5"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006678408.1"
                     /locus_tag="L3476_RS19390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L3476_19370"
                     /product="nucleotidyltransferase family protein"
                     /protein_id="WP_143798055.1"
                     /sec_met_domain="NTP_transf_5 (E-value: 1.8e-62, bitscore:
                     209.5, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKSLILDTDMLTNEMKLILEIISSDRPEDLRKDKAGLFQDCKWD
                     AFIQLAMHHRVYSVLYMQMKDMGDDMVPLAVIQRLYRQYCANTLRMLHLSSEIEHIGKE
                     LTGRGLRCLFLKGPVLGQDLYGSISLRTCSDLDLLVPMAELVRAETILITLGYEKDDYI
                     ESVLGDWKWRHHHLTFFHPVKGIKVELHWRLNPFPSKEPKFNELWLRKRRSTLFGSPIY
                     YLGREDLFVFLVTHGARHGWSRLRWLLDIKQLLNQQPDHGQIMNRLRRRRCVRAGGQSV
                     VLASSLLQAPLAPELQQLASRRRSVRLAQDAMFYLSQMVHLHKEPLPEEVRTYHKRHLF
                     ALMPLRHKCLFILSFMMPYPEDVKVIRLPRMLHYIYIPLRPFLWAWRKSAGVQK"
ORIGIN
        1 gcgccggaag catctcggaa tgggtgtaaa tattgatgcc cttgccctcc gtctgcttca
       61 gcaattcctc cagcgcatac aagttatgcc ctgtcacgac gatagacttg ccctctatct
      121 tgttctgcgg gacggtaacc ggcacgggaa cgccgaaatg ctccgtatgg gcccgatcca
      181 gcacatccat aatgcggatc gccgcgcccc cgaccttcat cgccatgtcc agatgctcct
      241 gtaagttgaa attggagtta gtcaacgtca tatagagcgc ttcatgcgta atcgcgtcaa
      301 cttcttcatc gacatagccc aactgccgcg catgcgtcgc atacgccgcg atgcctttca
      361 acgcaaacac gatcgtatcc tgtaaactgg caatcgtctc gtccttgccg cacactccaa
      421 caaccttgca gccgcccgtc ggcgtctgct cgcattgatg acagaacatt cgcgcttccc
      481 cctgtcaaaa tggatttcta tttccaacat actaaatggg aaagccacgc cgagtgcgta
      541 cgatcacaac aaatatttca aaatcatgaa tatcgcatca acttcacaat ctcgcctcat
      601 ctcacaaaaa aacagcgcgt ccattccgga tcacgctgcc catatgcgat tacaatatga
      661 gattacagaa ccgcttcctt catcaaggct tcgaccatct tctccaatgc ctcccgatcc
      721 ggttcctgga accggtccgt gagcggactg tcgatatcca gcacgccgac aagccgtcca
      781 tcctttatca tcgggacgac gatctcggaa cgggatgccg cgtcgcaggc gatatggccc
      841 gggaaggcgt gaacatccgg gacgacgagc gttcgccgct cctgggcggc gattccgcac
      901 acgccccttc ccaacggaat gcggatgcag gccggcaagc cctggaacgg cccgagcacc
      961 agttcttcct tggactcgtc cattaaatag aatccggccc agttaatatc cttcaagcct
     1021 tgccagagca aggccgatgc attggccagg ttggcgataa gattcggctc gccctcgatt
     1081 aaggcgagaa gctgcttatg aagcagcgga taatcctgat ccggctgacc ggaatattgg
     1141 ctcggtgtga acatcggcag gcctcctgat tgcacagtat ttcttgttac gattatgtac
     1201 catcgtatca catctggggg cttccgcgaa gaggcggtca gcgccgcaac gcctgtatga
     1261 ccaccctttt ccattacctc tccatcttca tttgcttctt ttgaccaata ttcccgtcgc
     1321 tcaggagagc cagtaacctt ttatagacca tacaggcctc tatgaacgat cctaccgtta
     1381 ttcagaaatg cccgaaaccc tctcgatcct gcacatatct cttgtcaaat atattcacgg
     1441 tgatcaggaa ctcagcaccc tgctctagcc ctcttataac ggatatcccc atagctcaga
     1501 aacgaatgat tttagactaa gcaggttcag gctatgcagg ttcaggctat gcaggttcag
     1561 accaagcagg ttcagacgaa gcggttctga acctggcggg ctcagaccaa gcggatagtt
     1621 tcatcctgac cgagtgggag attgagcgta gggtattgtt tcgaggaata tatgttacct
     1681 gaacgacagg gagactacac aaaagaatac gtaaggctgc aacaaatgaa aacaagactg
     1741 agcggatggg atattgagca aaaggagtga gaaagacatc tgacaagtat cagaagtgct
     1801 ccaaaaaacc gaaatcaggg aatgcgcaag gaatcagaag catggatttc cctccctgtc
     1861 cgaagatata atggaagata atgatataag gtcccttgag cgcaacgaga gaagcggacc
     1921 tgacgaaccg gcaataggct gctggccgct cccctgccgc cgcagcttat cgccgatcca
     1981 ttacgggagg cgttctatat gttatccaat acgttcacgt tcaccgaccc ggagggcgtt
     2041 cgcgtccatg tgtaccgctg ggatccggaa gaagggcagc ccgtgctcgg ggtcattcag
     2101 attgcccacg gcatgaccga gacggcccga cgctacagcc gcttcgctga agcgctgacg
     2161 gatgcgggct acgttgtcta cggcaacgac caccgcgggc atggccggac cgcggagcgg
     2221 cccgaagacc tcggctatgt cggcgaagac ggctttacat ggatggtccg caatatggcg
     2281 cagttgaccg agatcattca gtcggagcag gccgggctgc cggtatttct gttcgcgcac
     2341 agcatgggct cctttctggc ccagaaatat attgcggagc acggcgataa aataaacggc
     2401 gtcattctgt gcggcagcaa cgggccgcga ggacgcgaat tgtacgccgg agtcgcgctg
     2461 acgaagctga ttgcctccgc tcgcggctcc cgccaccgca gcaaaatgat tgacaacatg
     2521 gcattcgggg gcttcaaccg tacgttccgg ccgagccgaa cctccttcga ctggttgagc
     2581 cgggatgaac aggaagtcga taaatacgtg gccgacccgg aatgcggatt tttgagcaca
     2641 attggctttt accgggactt attcaggctg ctgcaggaca ttcatcgccc cgagacgatg
     2701 caggccatac cgaaggaact gccggtgatg ctcattgccg gcgaccgcga tccggtcggc
     2761 caatacggca agggtgtacg acggctggcc gagatgtacc gcgagctcgg gatccgcgag
     2821 gttgaatgca ttttgtaccc ggaggcgcgg catgaactcc tgaatgagaa gaaccgggat
     2881 gaggttacat ctgactgcct ctcttggctg cggaatcaca cgccgtaatc gttgctgcat
     2941 caaggaatat ccttcgacgt gaacggccga tttgtccctg catacttgga ggggtataat
     3001 ggaactggga agtccaatct cgaacatgat tctttggagg gatagccatg ccccaattat
     3061 ttgaaccgct aacgttacgc ggggtgacga tacccaatcg tatcgtgatg tcgccgatgt
     3121 gcatgtactc ttgccacaat gaggacggga tggtcaacga ttggcatctg gtccactata
     3181 cgtcccgcgc cgtcggacaa gtcggcctga tcatgatcga ggcgaccgcc gtcacccctg
     3241 gcggacgcat cagcgcgcag gatctgggca tttggagcga cgagcatgta gacgggctcc
     3301 gccgcctgac cgacatggtc cacgcgcacg gcaggaagat cgggattcag ctcgcccacg
     3361 ccgggcgcaa agcaaccgtt cccgggatca tcgccccttc ggcgcttcgc ttcagcgaag
     3421 cctatgagac gccggaggag atgacgctgg aggacatccg caagacgatc aaggcattcg
     3481 ccgacgccgc gcggcgggcg gaggaggccg ggttcgacgt gatcgagatt cacggcgcgc
     3541 acgggtattt gatcaaccag ttcctgtccc cgctcacgaa ccagcggacc gacagctacg
     3601 gcggcggcgc cgacgcccgc taccgcttct tgagcgaagt gattgaagcg gtgcgcatca
     3661 cgtggtcgaa gccgctgttc gtgcgcatct cggccaatga atatgccgaa ggcggcaacg
     3721 cgatcgaggc atacattgac tatgcgcggc gcatgaagga ccagggcgtc gacctgatcg
     3781 actgcagctc gggcggggtc gtccctcatc gggtcgaagc gtacccgggt tatcaggtcc
     3841 aatatgcgca tgcgatccgc caatcagccg gcatcgcgac cggcgccgtc gggctgatta
     3901 ccgaaccgtc cttggcggaa gagattgttc gcaatggccg ggccgatttc atcttcctcg
     3961 gccgcgagct gctgcgggat ccgtactggc cgcgccaggc tgcgaagcaa ttgcgccagg
     4021 aactcgctcc gccgaagcaa tacgatcgcg gctggtaatc catttacaat aatcattaca
     4081 tgttgaatcc acttattcca ggcggggccg cacgagcggc ctcgcctgct tgcattatct
     4141 tcgacccagt ccgctgctgc cgttcaagtg ccggagcgga cctctccgat cccgaacatt
     4201 tttaaggtcc ggacagcgcc cggaattgcc tcaaatcctg tcgttttctt atgaggaata
     4261 tcacctgaaa cctatttact tacccaatat gttgtagtat attatcatga gtataacaac
     4321 atattgaacg atacaggtgc tgcggttcga caaaagcgat acgcgtcggg ccccggctta
     4381 atagggaaga cggtgcgaat ccgtcgcggt cccgccactg taagaagcgc tgccggaaca
     4441 tcggtcctct ccgttccggc agcaagtacc ccgacagaac aggcgctctg tagagagcgg
     4501 ccttttctat gtccactggg acgaatgtcc cgggaaggct gccgaggtat tcggcttcga
     4561 gccaggagac ctgcctatat cgccgcacat tcatttccct acgcggaata gggaacgtgt
     4621 tcaggccgcg cgctgcgctg tgttaccgcc ttcggcggcg attgcagctg cgtggttgca
     4681 gggggagctg cgccttgtcc tagtgattgg cagccgccat gataagaagc ccccggatac
     4741 atacggcttt gccatgccgt cctgagctgt tctcgcgaag ggacggctat ttttattgct
     4801 ctttttcgag gaggaatacg gatgctaacc accaatcata ctgttcagac cgtacacaaa
     4861 ccgaataatc gctcgctcgc gttcgacgcg gagcgcatca tgcgttatgg cgagcgcatc
     4921 atgggcgctt acccggagct ggaccgggaa cgctggcacc ggggcgtcct ggccaagctt
     4981 cgtcacagcc atgttcaggc atccgatatc acgcaggctt gcatcatgac cgcattggag
     5041 ctagtctccg tcgaggagcc gaagtggaag ttcgtcgcgg cgcgcgccct gctgacgaag
     5101 ctgtataagg aagcggcttt gcaccgcagc tacaaggcgt acgccgatcg cccgtatggc
     5161 gacttctaca agctgattaa gcagctggcc gatatcggca tctaccgtca ggatctgctg
     5221 gaggcctata cccccgatca gattcgcgag ctgggccagg cgatcgatcc ggcctgcgac
     5281 gagctgttcg actatatcgg gctgcttact ctcgcggagc ggtatttggc cgttgatttc
     5341 gaagggcgca cgatggagct gccgcaggag cgatatatga tcatcgccat gtacctgatg
     5401 catcaggaac cggaggagcg ccgggttgag ctggccaagg aagcatattg ggcgatgagc
     5461 aatctttata tgaccgccgc gacgccaacg atggcgaacg cgggcaagaa ggtcgccggc
     5521 caattgtcga gctgcttcat cgacacggtg gacgactcgc tggaaggtat cttcgattcg
     5581 aataccgacg tggcccggct gagcaagatg ggcggcggca tcggcgtcta tctcgggaaa
     5641 gtacgggcgc gcggctccga tattcgcggc cacaaaaata cgagctccgg cgtcattcct
     5701 tggatccgcc agctgaacaa taccgcggtg agcgtcgacc agctcggcac gcgcaagggt
     5761 gccattgccg tctatctgga cgtgttccac aaggacattc tgtccttcct cgatctcaaa
     5821 ttaaataacg gcgacgagcg catgcgcgcg catgatattt tccatggcgt ctgtattccg
     5881 gatctgttca tggaagcggt cgaaaagcgc gaagactggc atctattctg tccgcatgaa
     5941 gtgaagaaga caatgggctg gaccgacggg aacggccgtc cgctaggact ggaagatttc
     6001 tatgacgagc aggcgggcca ggggcagttc cgcgagaagt atgcagaggc ggtggcgacc
     6061 ccccatctct cgcgcattac gctgccggcc atcgacatca tgaagcgcat tctcaaatcc
     6121 cagttcgaaa ccggaacgcc ttatatgttc tacaaggata ccgccaaccg ggccaatccg
     6181 aacaaggcac atggcatgat cttctgctcc aatctgtgca ccgagataat gcagaatatg
     6241 tcgccgacga cgatcgagca ggaagagctg gtgctggagg acggccgggc gcgtatcatc
     6301 atcagcaagc ttccaggcga cttcgtcgtc tgcaacctga actcaattca tctggcgcgc
     6361 gccgtgcggg ccgacgtgct cgaacgcctc gttccgatcc aagtgcgaat gctcgacaac
     6421 gtcatcgaca tcaacaatat cgaagtactg caagcccagc atacgaaccg gcgctatcgc
     6481 gcgatcgggc tcggcacctt cgggctgcat catctgcttg cgctggaagg catcgcctgg
     6541 gaatcggatg aggccgtgga atacaacgac tatctctatg agcggattca ttatttggcg
     6601 gtgcgggcaa gcatggatct agcgaaggaa aaaggcgctt acccgctgtt cgctcagtcc
     6661 gaatgggaga acggccgcta tttcgaacag cgcgaatata cgaccggaac gcgccccggc
     6721 ggcttcgtga cgaccgagca gtggaacgag ctcgctgccg aggtcaaggc gaacgggatg
     6781 cgcaacgctt ggctaatggc ggtcgcgccg aacggctcca cctcgatcat tgccggctcg
     6841 acggcgagca tcgatccggt atacgagctg ctcagctatg aggagaagac cacgtacaaa
     6901 atcgccaatc cggcgccaga cttgtccgac aagacgatgt ggtattacaa gacggcattc
     6961 cgcctcgatc agaactggtc gatccgcatg gcggggagcc cgccagcgcc atatcgacca
     7021 aggccagagc ttcaacctgt acgtccgtcc ggatatccgg gccgtcgatt tccttaacct
     7081 gcatctgcat gcctggaagg ctggcttgaa atcgacctac tacgtgcgca gccaagcgct
     7141 gaccgtagag gaatgcgaag cgtgcagctc ttaaatgaag tgaagcagcg ccgcatggag
     7201 ctctcgccgg gcggcagata gaatgatgat aatgtgagga aaggaagaaa atgatgactt
     7261 tactcgatcg cgagctgaaa ctgcaaaaaa tattcaatac cgaggcgccg aaccaatcga
     7321 cccgcattat tttgggcgaa tgctccggca tcttgaactg ggacgatatc cggatgccgc
     7381 acatgtacaa gctgtacaag gtgcttctgc tgaaccattg gattgccgat gagattccga
     7441 tgtccaaaga tgcccagcaa tttccgaacc tgacgccaga tgagcagcgc gcctttaaga
     7501 tcaatatctc gctgctggcg gtgctcgatt cgatgcagac gatgttcgta ggcgatgtga
     7561 agcggttttt caccgattcc tccctggagg ccatcgcggc cattatcgcc cagcaggaag
     7621 tcgttcacaa tcaatcttat tcttatgtgc tgtcctcgct cgtgtcggag caggagcaga
     7681 aggatatttt cgaatattgg aagcatgatg ccgtgctgct cgagcgcaac cgcttcatcg
     7741 cggacattta ccagacattc cgggacaatc cgacgccgca gacgttcttc gaagcgctcg
     7801 tcgccgatct gattctcgaa ggcgtatttt tctacagcac gttcgccttc ttctacaatc
     7861 tggcgcgcga ccagaagatg atggcgacga gccagatgat ctcgtacatc cagcgcgacg
     7921 agaaccagca ctgctacttc ttcggcgaag tgttcaagca gctgctgcgc gatttcccgg
     7981 aattgaatac cgacgcgaac cgccgattcg tccacgatac aattgaccgg gccgtgcagt
     8041 tagagacgaa ctgggcgcat tatacgctca gcaatattcg cggcatcgat ctgcgcgagt
     8101 tgagtgatta cattaaatat atcgccaacc gccgcctcgc catgatgggc ctggagaagg
     8161 cctatgaagg cgtcgacgtg aactgcatgc cttggattaa gcccttctcc gacgaggcgc
     8221 tcaattcgac gaagaccgac ttcttcgaag ccaaatcgcg caactacggc aaagtcggcg
     8281 atgacaacgg cttcgacgag ctgtaagcag gcatgttgcc gggcacccgc ccgcgcatgt
     8341 atccctattg ccagcgcata atatgaacgg tatgaggcaa ggccgagcag cggtattcgc
     8401 cagtaactgt cttgcccatt ccgtatagaa gctattataa tcaagctcct ctttaaaggg
     8461 ccacgtacgg tacacgcatt tccccgtgcc tgcgcggctc tttttttgat gaaaaaaagg
     8521 ccccatcttc ccagagaaga cagagggcat ccacgcgcct tattcgcttc cgttaaccga
     8581 tcaactcgac gacctcgcca tcctgcttgc ggatctcggt cttgacgatg ttgaatagca
     8641 ccttcggcag gtcgttacga ccgcttccta agaggggtct tacatccgcc acgtgatccg
     8701 ccacgtgctg cttccgccaa gcgctgcctt cgtcagacag gcagcagcga taagataatg
     8761 gaagtcagcc cgaagcagag attcagcagg cacaagaaga tgaccacctg cttctgattc
     8821 aatccgaccg caagcagacg gtaatgcgcc tgggtcgcat cggctttgta gatcggctgc
     8881 ccgttaagca tccgccggat gacgaccaaa atattgtcga aaatcggcac gcccaccgcg
     8941 agaatcggaa tgaagagcga taagagcgtc gcttgcttga acgcgccgtc gagtgccacg
     9001 acccccagca tgaagccgag aaacgtcgcc cccgcgtcac ccataaagac gttggccggc
     9061 ggcttgttat aacgcaaata cccgagtgcc gcaccaacca gaatcaaggc catcatcgcc
     9121 ggagtccctt gcccgtgcga gatgccgacg atgaacaggg tgccggccga gatcgcggtc
     9181 agccccccag ccagaccgtc catcccgtct gaaaagttaa taaccgttgt aaccccgaat
     9241 atccaggtta ccgtcagcag gaactgaagc cattccggca agtaaatgaa ctgccccgtg
     9301 aacgggttgt tgaagccgat gaaggcgatt cccgaagcat agaccaaagc ggcggcccct
     9361 agctgcacga taagcttcgg cagcgcgggg aagtccttgc gccgagtctt gtaccagtca
     9421 tctaccatgc cgatggacag gatgagcagc ccgccgccga gaatgccagc catcttcgtc
     9481 aaggaatgcg gaacgaataa cacataagtg acgcaaaagc tgataaaaat cgcagcccct
     9541 gccaacaggg gaatgggctg cctatgtatt ttccgttcgt tgtccttctg cggctgatcg
     9601 acgaagccga tgcggaaggc taattttttg aatggaggaa taagtaaaag gacaatacag
     9661 acagaaagaa ggaaggcgaa aacgtaataa aacatatcat tacctccgct gcataaccgt
     9721 ttacgttcat tataaagcat atatgaacat gatcaaccac aaggtgacat attgcgccac
     9781 ctcgaatggg atggcttaca cgggatgcac tggccgagac gaggctgatg ttcacatcta
     9841 tttctacatg tgcagttccc gccccttatc attctttcaa ccaaggttga caatccttaa
     9901 agagggatgt tactctttat ttgtgttctt tattgggaac acatcaagtt tatattgttc
     9961 tttaatgaga acaaaaacgt gtattcggag gagaacgatg atgggtgcgg tagcaggcat
    10021 ttatcacctt caacacgata agctggaacc tggacttggc agacaaatga tcgatgcgtt
    10081 gcggcgcttt cccgctgatt atgttagcag ctggagtaac ggttccttat ttctggggag
    10141 cctccagcag tgcattacgc cgcaatccat caacgaggtc ctgccctgct acgatccggt
    10201 tacatcgctc gccattactt cggatgcgat tattgataac cgggacgaat tagcagacca
    10261 gctgcaacta tccaagccgg ctcgacagac cataacggac agccagctta ttctccaagc
    10321 gtatatgaaa tggggccatg atatgcccaa gcatctcgtc ggggatttcg ctttcatcat
    10381 ttgggatggc agggatcgca cgatgtttgg cgcaagggat ttttccggaa caaggaccct
    10441 ttactttcat caatccccgg aactgttctc attctgtaca atcatggaac cgctgcttac
    10501 gctgccacat gtgaaaagag acttgaacga acactggatt tcccaattcc ttgctatccc
    10561 catcacgaca gacgccgtag attctttttc taccgtgtat caatccatca agcagatccc
    10621 cccttcgcat tccattttcg ttgccaatgg gaaagtaatc tttcaacaat attgccgtct
    10681 gcagcatact gagaagcttg tgcttacaag caacgaggaa tacgaggaag cgttccggga
    10741 tgtttttcag actgcggttc aggctcggtt aagaacgttc cgccaggttg gagcacatct
    10801 aagcggcgga cttgattccg gttcggttgt aagctttgcc gcaccgatgt tgcaagccaa
    10861 caacaagcct ctctatacct tcagctatta tccgatcgaa ggcttttccg atttcacttc
    10921 atggagccgt tttgcggatg aaagagaata catacggtcc acggcggagc atgtaggcaa
    10981 tatcgagatg aatgtatcca gttatccgtc aagcaaccct tactccgtca tagacgaatg
    11041 gcttgatacg ctggagatgc catataaatt tttcgagaac tctttttggc ttaaaggaat
    11101 ctatgaacaa gcctctgccc gggacatcgg ggttatgctt accggacaga gggggaactg
    11161 gaccatttcc tgggggcccg ccatggatta ccaagccaat ctgctaagga aggggcgtct
    11221 cgtgtcgttc ttctgggagc acagacgtta ttgccaatcg cttggggcga atccctggaa
    11281 agtgcttcaa atcgtcggca aaaagagctt tcctaggctc gccccgctga ttgctccggg
    11341 aggcgaccct gcgctgccgg tcttgattaa cccggacttc gcccgcaaaa ccaatgtaac
    11401 cgaccacatg aaggcctgcg gcatggacgt ctggggatca tcgactcaga acgcgtatga
    11461 catccgcaag gatcatttta atcagctgta cttttggaat attaacggaa cgatcggcac
    11521 gaagctttcc cttaagcata aaatttggga cagggatccc accaatgatt tgcgggtcgt
    11581 ccgcttctgt ctgtcggtgc ctgatgaaca gttcgttcag caaggcgtcg accgttcact
    11641 catacgcagg gcaaccgccc gctacctccc cgacaaggtg agattgaatc gcaaaagacg
    11701 cggcgtccaa ggcgctgacg gcgtgttcag gctcatgcct gactggagtg gattgatccg
    11761 ggagcttgaa cagatgattg gcgatccgcg aacctcttat tatctgaatg cccccctgct
    11821 tcgtgaactg ctgaacgaga tccgccatcg cccgcggccg gagcttgcgt tcgatctcaa
    11881 gttccgcgtg ctgatgcgcg ggttcatctt tcataaattc ctgtcaagaa tctctggaaa
    11941 ggaggtgtaa ccatgaaaaa gcagtacagc aagccatccc ttgaagtgtt ggatgttcat
    12001 cagaccatgg ctggaccggg tctctctacg ccggatgctt tccagccgga cccggatgaa
    12061 gatgtgcatt acgatccaat ggatagctag acaattggct taatgaatgc ggttactggt
    12121 gcggtaccct tatacatctc aacgggtata agggttcccc tgttctcatt ttacaaactc
    12181 ttggagagat gagcatgacc gaacgagcag cggtaaggac tgaccattat aaggcttttg
    12241 gcttccgtat tgagagcgac tttgtattac cggaactgcc tctggctggc gagcgcgagc
    12301 ctctggataa cattacggta cggcggacag acttgcagcc tctctggaac agctccattc
    12361 atttctatgg aaacttcgct attctggatc acggccgtac ggttatgttt cgggtgcctg
    12421 gggctgccat atatgcggta caggatgcct cctccatcct ggtctctccg ttcgatcagg
    12481 cggaagagaa ctgggttcgg ctttttattc tggggacttg tatgggcatt ctattgctgc
    12541 agcgcaaaat tatgcctttg cacggcagcg ccgtagctat tgacgggaag gcctatgcca
    12601 ttatcggcgc atccggtgcg ggcaaatcga ccttggcctt gcacctggtg tccgagggat
    12661 atccgctgct tagcgatgat gttattcccg tcgtgatgac tcaaggctct ccttgggtcg
    12721 ttccttctta cccacagcaa aaattatggg tggacacatt gaagcatatg ggaatggaca
    12781 atgccaatta caccccgctg tatgagcgta agacgaaatt tgccgtgccc gtggggtcca
    12841 actttcatga agaaccactt ccgttggcaa gtatctttga gctggttccc tcggatgcag
    12901 ccactcatat tgcgccgatc caggggatgg agcgctttcg cgtcctcttt caccatacgt
    12961 accgtaattt gttggtacag ccgctgggat tgatggaatg gcacttcaag accctgtcat
    13021 ccttcattcg ccaggtcggg atgtaccgtc tgcaccgtcc catggttgga ttcagcacgc
    13081 ttgatctgac atcgcacatc ttcaacatta cccggcaagg agagaatgac caatgagcaa
    13141 acttcattcg atcacccctg tcgatacgct tgttcaatgc gagggccata tcgttagcga
    13201 tatggccggc gaaaaagtaa tgttaagcgt ccagaaggga aaatactata atctcggtac
    13261 gcttggcggc gagatctggg acatgcttat cacgcccgtg aaggcggaac atattatccg
    13321 atccatttta tccgaatatg aggtagagtc gtcggaatgc gaggaagaca ttctcctgtt
    13381 cctctcggat ttggagcatg agggcttaat acggcatgtg aagcggctat gaaccggttg
    13441 cgaagatgga tatggttatg cttatcagat cggaagacat tccttttatt cgcggaagct
    13501 tttctttatt tggggtgggc gcgcatactt ttgtatttcc cgttcgcgag gatctcgcct
    13561 ctgttgggga gacgatccgt ggaaacgcca gaagttcatg atccgtcgca gacgaaaacc
    13621 atgaagcata ttcggagcgc gatccagatc gcgagccggc atacgttctg ggagagcaaa
    13681 tgcttggtaa gggcgatagc aggtatgaag atgcttgaaa aaagaaacct gcacagcacg
    13741 ttgtatttgg gaaccgggaa aaataaaaac ggtgaactga tcgcacacgc ctggctccgc
    13801 agcggctcga tatacattac cggcgccgag atgatgaagc agttcgtcgt ggtggagaaa
    13861 ttcgcgaaga gcgcagggac agacccatga attcgattat tttgtacatg aagcggcttc
    13921 ataaaacagc agggttacaa ttaagcatca acattatcgg catggtgatc agcagtctcc
    13981 tggagggaat gacatttctt ctgctcatcc ccatgcttac ggttggcggc gttctgttgt
    14041 cagagccggc ttgggagaac aggctgcggt ttttgaacat cctgcacagc ttgccgcagg
    14101 cctcttccct ggccatcatt ctaaccgtat acctgctgat tgtcgtcact caaaacctca
    14161 ttcatcgggc cgttacggtc cgcaatgccg agatagagca gcatttcagc cgacaactgc
    14221 gttacgagaa ttacagcgct cttctaagag cgcaatggtc ttttttcatc aaccgcagaa
    14281 cttccgatct ggtgaatgcc cttacgatcg aaaccgccag agtcttgggg ggaatcagtc
    14341 tgcttctcca attcattaca tctgccttgt ttaccctcct tcagattatt ttcgccttct
    14401 ggatctcacc gaagataacg ctgttcgtta ttgcctgcgg agccatgctt gcttttcttt
    14461 cccgcggatt tattcgcaaa tccaagcagc tcggcagcca gacgtccaag ctcgcccaag
    14521 cttatttggc aggaatcacg gaccagcttc aaggaatgaa agatataaag agcaataacc
    14581 tggaaccctc ccgcttggag tggcaccagc agcttaccga aggaaccatg caagagcaga
    14641 tggattatgt gcggctacgg ctgaattccc agctgttgta taaaatagcg tcctcactgc
    14701 ttgttgccgg atttatcttt acgtcatttc gtttttttcc gagtgaaggg ccctctttat
    14761 tgatcgttat cctgctcttt gccaggttgt ggccgagatt taccagcctc cagtccaagc
    14821 tggaacaatt ggcctcgacc atcccggcct tcaaggctct tcagcaattg cagcgtgact
    14881 gcaaattggc tgcagatcag gcgattacgc ttaccgaatc gcccgctccg ggacctggcc
    14941 cttggttcgt tatgcaagga atcgaagtca gaaatctttc gtttcgctat cagccgcaac
    15001 tgcctgtgta tgctttgcaa aatatcagcg ttcatattcc agcccaaggg atgaccgcta
    15061 tcgttggcaa atcaggggcc ggcaagagca cgtttatcga tatccttatg ggcctgatgc
    15121 agcctgaatc cggtcaaatc ctcgtggatg gcatagcgat cacgaatgac aacctgctcg
    15181 cgtacagacg ctccatcggg tatgtggcgc aggatccgtt tctctataat gcgaccataa
    15241 gagaaaacct gttgatggtg aaaccgagtg ccacggagaa cgagctgtgg gaagccctcg
    15301 atttttcctc ggcggctgaa tttgtacgca agcttcctcg ccaattggag acgctcattg
    15361 gagatcgagg cattcgtttg tcaggaggag aacggcagcg cctggtcctg gcaagagcca
    15421 tcatccggca gccttctatc cttgttctgg atgaggcgac tagcgcactg gatacggaaa
    15481 atgaacaaaa gattctgacc gctttacatc ggttgaagga tcgcattacg gtcattgtgg
    15541 ttgcccatag gctgtccacc atccagaacg cggaacaaat tttagtcata catgacggga
    15601 aattggttga acagggaacc tttttgtcat tatcctcaga aaaaaagagc atatttgcaa
    15661 atcttttaca aaatcaggag gcgcagcctg ttaacgtcta tacgctgtag ccgctttcgg
    15721 aggtggggga aatcggatat aatagggaca agcagatgta tacacggagg ttaaatcatg
    15781 atcggagaac ggattcaaat ccttcgaagg aagaaaaatt actcactgtc agaactctcc
    15841 gaaagagcag gtgtcgcaaa atcctattta agctcaattg aaagaggaat tcagcagaat
    15901 ccttccattc aatttctgga gaagattagt gctattctcg gggtctccgt tgaggagctg
    15961 ctgcaagaca aggaagcttc gaatgaagct gaggcgttag acgaagaatg gactaatctc
    16021 gtaaaagaag cgatggcttc tggcgtaagc aaagagcaat tcaaagaatt tctggaatat
    16081 aataagtgga agctaacacg cgaagagtcg tgatgctccc tagcgttgac gcagcccaaa
    16141 ataaagaaat ctccgattgt tgagttcacc gcgcgttatc tgtaaacatg cctcaacgaa
    16201 ggagatttct tttcgataat gtcaacaatc gtgagttatg cttctgaatt gacatggagc
    16261 ggccattcgg gatcagactg tgcctgaaaa tcgcgaagaa gtttacgaat gtccgcaatg
    16321 gaaaggccca tttgtcgcgc agttagaata atagctaccc aatccttgtc caattgttca
    16381 ttgttacctg taacggcttc aaccatgatt gcattgcctc ccgaatttac tcggccggtt
    16441 gcaccactaa ctccacacgc ctaaagtgcc cagacaaaaa aggcgcattt catcgtttcc
    16501 taatatatac ttattaagta gtattataca cgacatttga cgttccatgg tgtcatagta
    16561 tgtcgtctct ggagcactag ttttgtcgaa ttggatgatt acccggccgt gatcctctcg
    16621 caataacctt gcggattgtg aacctgccaa ttccagctat cctcacacat gtcggtaatg
    16681 ccgtatttcg ggaaccaccc caggcttata tgggactttg tcggatcagc atagcatatc
    16741 ccgatatccc ctggtcttcg ccccacaatt tcatagggaa tctgaatccc cgacaccttc
    16801 tcgaaggaac ggatcatctc cagcaccgta aacccacggc ctgtacccag attgtaaaat
    16861 tcggccccat tggtatgatc caaatgctcc aatgctttca gatggccttg cgccaaatcc
    16921 acgacatgaa tataatctcg gattcccgtt ccgtcttttg tagggtaatc ggagccgtat
    16981 actttaagcg acggcaatct ccccacagct acttgcgtaa taaacggcat taaattgtta
    17041 ggcgtcccat tggggctctc tccgatcttt ccgcttttat gtgcaccgac gggattgaaa
    17101 tatctgagaa tggaaattct ccatctcggg tcagaagaag ccagatcctg cagtacttgc
    17161 tcaatcattt gtttggttct tccataggga ctcaccgccc caaggctggc ttcctccgaa
    17221 atgggaacac attccggtac cccatacacc gaggcggatg agctgaatac caggttgtac
    17281 acgccatgct tggccatcgc cctgcacaga accagcgtgc tggtaagatt cgtatggtag
    17341 tattcaagcg gcacctgaac cgactcccca accgctttca ggccggcaaa atggatcacg
    17401 gcatcgatgc gatgtccctc gaatatccga ttcaagcgtt gcccgtcgag aagatccgct
    17461 tcataaaaag caaatccttt gcccgtaata tccgaaatcc gacgaatcgc ttccggatgg
    17521 ctgttggaga aattatcaag aacgacgata tcatatcccg cctccagcat ttctacgcaa
    17581 gtatggcttc cgatataacc ggcgccaccc gtgatcaaaa tggccattac cttgtctcct
    17641 ctctccgtat gaccggattg aatttagtaa ggatcggcag ctttcccaga ttcgccttag
    17701 ctatgttcat taagtagata aactcgctcg atttccgaaa gcacacatag aagatcaggt
    17761 tcgttacgga caggcacagc aggaccctga cggcgatatc ccataacagc ggcagcggga
    17821 gtaatcctgc aagccagccg cataaggatg ccgccagaag aactaacgcg gcatgcagtg
    17881 cgtaacgggt aaaataaggg cgcagcggct gcaagaatac gtgcttatga agcaaataag
    17941 gatcaaccca aaaataggtt agcaatcggc taatgactgt tccgaggaac actcccggca
    18001 tcccgatgaa gcgtgcaagc gtaatcgata atccgatgtt cattgccgcc gcgatcaacg
    18061 ggctgtacct tccccttcgg aacatgcccg tcgtatcccg gaacatggtg gaagcatttt
    18121 gcatccccgt cgtataaaaa ttgagcagga tcgcaagcaa ggtctccttg ccgaggacaa
    18181 aagaacctcc gagccataaa ataatcacgg gatcaagcaa attccacaaa catacggcac
    18241 taagcccgaa cacccaaaag ttggccaagc gtaacacacg atagaagaaa aacttcttct
    18301 ccttatcctc cgtagcgttc aaattcccca cgctggccgt tagagaatag aagaaatagc
    18361 tcagaaatgt cgttaaggtt gtcagaatca aaaaataatt ggagtacagt ccgacccata
    18421 tgacgccaaa taggttggca ataatcagat tatccgtccc attgatgacc acgccgctca
    18481 ctttgtacat aagcagggag cgcaggtttt gaaaaaaggt cttcttctct cctttgtcca
    18541 aaggagtgct gaccggctcc ctcaggaatt cgtactttcg gtcggccaca cgggaaatat
    18601 acttgtttct aagaatgctc attccaattt ggatcacaag gaatagaatg tagtttttcg
    18661 tacagagaag cacgatgatc tgaaccgtgt tcatggcaat cacatagaca ctatggattc
    18721 gcgatatcac atgctgctgc tggtcggcca caatgatggc ctgtttgtaa gcgaagaagt
    18781 aggtggacac ggaattgagc agaaataaca catagatgac ttcgatatga gaaaccgtcg
    18841 tttccccctg aatgaaaaca tcaagaaaag gaatcaggga aagccccagc acgagcacaa
    18901 gcatacctat gaacagatag gcttttcgaa ataaatgcat aaaagctttt attctggcct
    18961 cgtcccgatc ggcaagaggt tgatacaagc tgtaaatgat ggcagtctca aaaccgagat
    19021 tggctagaga caggagcatg agcacatcgg aaaacagacc gctcactccc agatactcaa
    19081 cgccaagcgt ccatataaaa accgttctaa cgataaaacc cattatggtg ctaatggctt
    19141 gccccagcag accaaaaaaa atgttataaa gcgaatttcg aattctcatc cctgtacctg
    19201 ctcttttacc tggtttcacg gatttgctgc aaacccgttc tcactttcct tctgatgatc
    19261 gggaaaaatt gcgtcacacg gtagcggaac cgttcttttc ttcgatgggc cacaaaatcg
    19321 taatccggaa accaagacag cagatcctga tccttcacgt tgtcagggtt ctcccgcatg
    19381 cgctcctgga tcggcttcca taaccgttcg atttggatat tcgtgaaaaa cttgacctcc
    19441 cgcttcaaaa accgcttcag aaaaccggag agctcatcag gcaccggaaa accgtagtca
    19501 ttcgggtcat ggccctctgc cgcaatcccc atgatcgttc ttacgcactt ctcgcattgg
    19561 cagcagtttt ctgtgttgcg atagcatacc cggacggcgg tcttgtcatt ggcagcggca
    19621 taatgatcga caaccacttt gactttatcc tgccgggaca gctcgtatcc gtcatggaac
    19681 acatcgcccg ctccgtaacg aatcagattg tccgtcgatg ggtcggaagc acatgaatac
    19741 gtgttgccga tcgtatgcga gctcgcaatg taaatcgttt tcacctgtaa taagcaggct
    19801 gcaggaaccg cagccgatat aatcgccaga ccgtgatgca gtccatgcca ccaggtgtcc
    19861 cccagcgaac gccggaaatc ccggtcgatt cgctgaggat agacgaaggt gccgtagttg
    19921 gactccacca ggatattgga gagcccttcc ttgcgggcaa aagcctccgc atgctcacgg
    19981 tctgccgtcc atacctcgct gtctgtacag tccccttcat gccatccgta ctccgtaatg
    20041 agcaaaggtt tcttatcccg gtgacggata taggtcgtca atgcgtccaa tcccccgcta
    20101 aatagcacgg cagcttcatg ctccggttga taatgatagg tagaaacctg cccgaccttg
    20161 atttgcccct tgagcgggaa atgggggaac atctcctgat atgcctgctt cagttccgca
    20221 agacagtcat agaaggaacg atccagttcg tcgacgtaca gtgtcgcatc cgtaagccac
    20281 gcgagcggca tcatgttcgc cgcaaacgga atcgacaata tgcttgctgg aagacccgag
    20341 atatcatagt tatatttcat aaataaatga ttgcttgctc tgaagtattt ttgaagcctg
    20401 gggtccacct gaaaaaaata gtccacacgg ttctgattta ctttgatttg ttcccgtgtc
    20461 agttgtatca tgggcttcct ccttcaagac cggtattacc gctgcttgaa ctgatccagc
    20521 catgcttgga tggcctccag gttcatccgg acattcgtcg caaattccga atggatcatg
    20581 gcttcaaggc cggcgagatc cgtacggtca acggtattca gggcgtatgt ctcactcatt
    20641 cctctggccc gttcgtcaac cgcaataatg atggaacgtt tcttgtgacg catcgcataa
    20701 attccggcat gcaggcgcgt gcctacaaag tcgacatccg tgctgagcac agcttcatat
    20761 tcttccagcg aaggaggaat aacgtgaatg ctatcggtat tcgataacgt atgaaaatac
    20821 gcataatcat cggctccctg aacccagaaa tatactttcc tgtagcttct aatcagaaga
    20881 tcgatcagga gctgatcttg acggggcgct cttgcgtaat ctgtcaacgt aaacacgacg
    20941 gcatccgatt tccccttagg aatatcctgg caatgctcgg gcgttaacgc ccacatcgtc
    21001 ggacatcccg tatttaacgc cttgagccct aaacccgtga cgaatctcca ggttcgttca
    21061 tctcgaacgc tatgcacata ttcatgggac aacaccttgc ggtacagcat tttcgtgtac
    21121 cgttttattt tttctccttt gcccgccccc acccccagga gaatgctgcc ttgtaacgga
    21181 cgataattaa acacattaat attccattgc gggaaatggg tgaacatgtc catagtcaat
    21241 agattcgttc cgccgacgaa cttgtatttg gctcttgtat agaattgaac acgtaatgaa
    21301 tctcgcatca cctggaacca gccgaagggg gaaagatgtg taggcacggt aaacacattg
    21361 gcctggctcg taatgccaga caattggcgc ctcacgcatt tcatgatgat ttcatcccct
    21421 ttattcaagg aaccgactga ggtgtcgagc agcaggatgt tatccatagc ctcgcctcct
    21481 atccattgtc atccaacaat gcgtatagtt tattgagttc gcccgcattg ccatactgag
    21541 tcctgctgca catgtccgcg agccggcatc ttagctgccg atctcgatat aaatgctcaa
    21601 tcccttccgc taagccctcg accgataagt ccgtaatata tccgtccaag ccgtcggcaa
    21661 cctgactctg tgccgtagga tagcgggtaa tcagaacagg tttgcccaaa atctgggctt
    21721 cggttacggt cacggctttc ccctcgtaac gggaaggctg tacatagaga tcggcttgct
    21781 tgatgaaagg atagggattg tactgcttgc ccagcaaaat gaaatcctcc tgcagccgat
    21841 tcttcgcaat gagaccgcga atcatctcct cgtccccgcc ataacccaca acgtaccagg
    21901 caatgccctc aagccccttc tctctcagct tcttcagagc catgacagcc tggtcgattc
    21961 ccttggcgtg cgacaaacgg gcgacggtga ccaatttgaa gcgcggatcc gctgccatcg
    22021 gtgactcgac agacacctcg gacatcatct tgatgtagtc cggagagata atgttctcga
    22081 tcacagttac cttgtccctc agggattcgt acttcgccaa gaaggcttcc ctgcatgaag
    22141 gagatacggc aatgatatga tcgaacttgc tccacagctt caaatcagag cgcacattcg
    22201 tagctacggt cgaataatcc gtatgaatcc aggctatttt cgtgcgggca tcgactttct
    22261 cggcaacgta gtaatggggc cataagaagc ttactgccgc atcgtagagc tgtttgttcc
    22321 ggggcaggaa gggaagggta tatctccaca tcagctgcat ctgatgatat cccgtttctg
    22381 cacaccctct cgctttcccg gccatatcgg catgcacttt tgcggtcagc cttccgagcc
    22441 cgatcagcag ctgccggctc ttgataatgt cgccgatgga cttgcggaag gtggcatagg
    22501 ctcgttgctc ctccagcaac cggacttgtt cggggagcaa aggcataaaa tcgccttgat
    22561 ggcgatacag catcagctcc gcctgatagc gttcataatc aaaatgctcc agcaaaccag
    22621 ccaggcttct ctcgactcct ccaacctcca tatcgaagga tgcgaacagt actcttttca
    22681 tttctgtaca cccgcgcttt ttctccatgc ccacaagaag ggccttaggg gaatatagat
    22741 ataatgaagc attctcggca atcgaatgac cttgacatcc tcgggataag gcatcatgaa
    22801 gcttagaatg aacaagcatt tatgccgcag cggcattaac gcgaacagat ggcgcttatg
    22861 gtaggttctg acttcttcag gtagcggctc cttatggaga tgaaccatct gcgagagata
    22921 gaacatggcg tcctgggcca gccgcaccga tctccgcctg cttgcaagct gctgcagttc
    22981 cggcgctaac ggcgcttgaa gcagcgacga tgccaggacg acagattgtc ccccggcccg
    23041 cacacatcgg cgccgcctca atctgttcat gatctggccg tgatccggct gttggttaag
    23101 aagctgcttg atatcgagca gccagcgcaa ccgcgaccag ccgtggcggg cgccgtgggt
    23161 cacgagaaag acgaacaaat cctctcttcc caaataatag atcgggctgc cgaacagcgt
    23221 gcttcttcgc ttcctgagcc acaactcgtt aaatttcggt tccttggacg ggaacgggtt
    23281 cagccgccaa tgaagctcaa ccttgatgcc cttgaccggg tgaaaaaagg tcagatggtg
    23341 gtgccgccat ttccagtccc ccagtacgga ctcaatgtag tcatctttct cgtaccctag
    23401 cgtgataagt atcgtttccg ccctgactaa ttcggccatg ggaacgagga gatccagatc
    23461 cgagcaggtg cgaagcgaga tacttccata taaatcctgt ccaagcacag gtcccttcaa
    23521 aaataaacaa cgcagacctc ttccggtcaa ttccttgccg atatgttcga tctcgctgct
    23581 cagatgaagc attcggagcg tattggcgca atattggcgg tacagacgtt gaatgaccgc
    23641 caagggcacc atatcatcgc ccatatcctt catctgcata tacagcacgg aatagactcg
    23701 gtgatgcatc gcaagctgaa taaatgcatc ccacttacag tcctgaaaga gcccggcttt
    23761 gtccttgcgc aaatcctcgg gacgatcact tgaaataatc tccagaatca acttcatttc
    23821 gttcgtcagc atgtctgtgt ccaagataag ggacttctcc atgaacttac tccttcagtc
    23881 agatatcgct
//
