LOCUS       NZ_CP116962            10882 bp    DNA     circular CON 10-MAR-2024
DEFINITION  Enterococcus faecalis strain DM86 chromosome, complete genome.
ACCESSION   NZ_CP116962
VERSION     NZ_CP116962.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2959291)
  AUTHORS   Shen,W. and Cai,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) Clinical Microbiology Laboratory, The Second
            Affiliated Hospital of Zhejiang University School of Medicine,
            Zhejiang University, 88 Jiefang Road, Hangzhou 310009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/10/2024 16:14:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 29 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 6 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:47:18
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1761085
            Orig. end                         :: 1771967
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116962.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10882
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10882
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10882
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5882](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5882)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(792..932)
                     /locus_tag="PO880_RS08440"
                     /old_locus_tag="PO880_08440"
     CDS             complement(792..932)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386764.1"
                     /locus_tag="PO880_RS08440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_08440"
                     /product="hypothetical protein"
                     /protein_id="WP_002386764.1"
                     /transl_table=11
                     /translation="MFRWLPVIAGIATKDEVEIATAEELAVWNEVAYQKINLTKSRGGV
                     I"
     gene            complement(973..1305)
                     /locus_tag="PO880_RS08445"
                     /old_locus_tag="PO880_08445"
     CDS             complement(973..1305)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377523.1"
                     /locus_tag="PO880_RS08445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_08445"
                     /product="hypothetical protein"
                     /protein_id="WP_024797061.1"
                     /transl_table=11
                     /translation="MTKKDEVKELEAKNNIPEAEKKPFSKFGKQEKHTVEDVEYTFQFP
                     GTRAAQAILDNSKGPSNTFSDVAYHSQLMDSVIVTPKLNWDYWDEHEGYREVMALADNF
                     LGRMLN"
     gene            complement(1358..1756)
                     /locus_tag="PO880_RS08450"
                     /old_locus_tag="PO880_08450"
     CDS             complement(1358..1756)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010711287.1"
                     /locus_tag="PO880_RS08450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_08450"
                     /product="hypothetical protein"
                     /protein_id="WP_002377526.1"
                     /transl_table=11
                     /translation="MQSMTTYDAKEVSTIIDNVVQFGFQDGDMVSFSKDNSYIEVQTDA
                     QGQSSAAKNNDNLGTFTINLSQNSPCNKQLMALANGRKEFAISVTHSTEKAWASKAYIE
                     KTPDGSFGKGVPTRSYTIKALDYKHEYN"
     gene            complement(1772..2809)
                     /locus_tag="PO880_RS08455"
                     /old_locus_tag="PO880_08455"
     CDS             complement(1772..2809)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717321.1"
                     /locus_tag="PO880_RS08455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_08455"
                     /product="DUF3383 family protein"
                     /protein_id="WP_113844220.1"
                     /transl_table=11
                     /translation="MIEKITDVNVKIDIMHPQPIVGLGNPAIFVQGSTQNYKEYTSLET
                     LAKDFATTTTVYKKAEIIWKQENKPQTIAVVTFVADNPTSPEQPDSLIAGSGIIAAATS
                     YFYNDWHFALLANFVEADALALSNLIEENEFKFLVIQTATVDELTVFTGNNLTIGLVHP
                     LEEFLDAALIGNTASLTVGSVTWKFRHNLVGITPNTLTTSQLQAIEKANAIAYVSKAGI
                     PQTSEGKTMSGEFIDALHGDHWVKSNIETNVQRLLSTTDKLTFDSNGIALLDTTVANVL
                     ETAFNNGIVDIVDETGVGNYSVTALGRQDLNPDDIAARNYKGLSFKYKRSGAIHTVDVT
                     GTIEV"
     gene            complement(2806..3273)
                     /locus_tag="PO880_RS08460"
                     /old_locus_tag="PO880_08460"
     CDS             complement(2806..3273)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425319.1"
                     /locus_tag="PO880_RS08460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_08460"
                     /product="hypothetical protein"
                     /protein_id="WP_113844219.1"
                     /transl_table=11
                     /translation="MTESYNYGLLANKLIEVVQKGTGCQLIEGGTAGPQPDKPFFSYEV
                     ISPYIPVTIDVTDNEVFELVVSIKCHTDSSIQALNLSEQLRKHLNGFSVKVDLQNSKIA
                     LVSTTQSQKRDNFISIDYERLAGFDARFRVQDSYVDNAVIIENIDIQEENK"
     gene            complement(3266..3640)
                     /locus_tag="PO880_RS08465"
                     /old_locus_tag="PO880_08465"
     CDS             complement(3266..3640)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386758.1"
                     /locus_tag="PO880_RS08465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_08465"
                     /product="hypothetical protein"
                     /protein_id="WP_272731389.1"
                     /transl_table=11
                     /translation="MQKLDFTSVLASFGIPITLLVKPEKEGTYVHGEWFQTPYESWEKR
                     ELNDPVIPSSLITQLPMQAKYGDGGRYEEYDLIWFSSEQVPIKSRILKENRVYSVESIT
                     PYTDYSNVMQYGLKAVQGHD"
     gene            complement(3640..4242)
                     /locus_tag="PO880_RS08470"
                     /old_locus_tag="PO880_08470"
     CDS             complement(3640..4242)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774206.1"
                     /locus_tag="PO880_RS08470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_08470"
                     /product="hypothetical protein"
                     /protein_id="WP_113844217.1"
                     /transl_table=11
                     /translation="MKINEINRIPQIIKELSLLKRFVIQIGIFGSDDSFMAMIANVHEF
                     GCTIKPKGKYLTIPLSKKYKGKSPRDFDLFFMQTKEGHKFLVMNKGKEQLEFAYMLTEK
                     ITIPERSFLRSTFDEKNKEWSDYSLTLVKKVVDGELTANELMNHLGLRIQRDVQRTIRN
                     LSDPPNSPITIHNKKSSNPLIDTGRLRQSVTYKVVKG"
     gene            complement(4242..4583)
                     /locus_tag="PO880_RS08475"
                     /old_locus_tag="PO880_08475"
     CDS             complement(4242..4583)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377536.1"
                     /locus_tag="PO880_RS08475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_08475"
                     /product="DUF4054 domain-containing protein"
                     /protein_id="WP_002392022.1"
                     /transl_table=11
                     /translation="MPKSTVENVRLTAAELAGVNNDSIKLFIDDAWLEVDALPFKEDVK
                     EKACRYLACHLAVLNNQNTKSEQVGSLKKEYSGFHSTFTDLKRTVYGQEYLRLYNEYAK
                     KGSLSLVVI"
     gene            complement(4595..4981)
                     /locus_tag="PO880_RS08480"
                     /old_locus_tag="PO880_08480"
     CDS             complement(4595..4981)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377541.1"
                     /locus_tag="PO880_RS08480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_08480"
                     /product="hypothetical protein"
                     /protein_id="WP_113844216.1"
                     /transl_table=11
                     /translation="MLVHNKGAYIRHIGNVRLIPGVNDLDNSDAEEFIKGMELPLNKSL
                     ERLGEIEILDHITKGKSKKAVGFTELTANKAVESIADTFDLELLEKWLEEEQANKNRTT
                     VVKAIENQIDDIKNPDEDSVVNPE"
     misc_feature    complement(4958..4974)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.71"
                     /tool="antismash"
     gene            complement(5001..5882)
                     /locus_tag="PO880_RS08485"
                     /old_locus_tag="PO880_08485"
     CDS             complement(5001..5882)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377543.1"
                     /locus_tag="PO880_RS08485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_08485"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_010733792.1"
                     /sec_met_domain="Linocin_M18 (E-value: 2.2e-50, bitscore:
                     168.6, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGNDVTATLEARDLQEIDKVIYQAPQEELVARTMFNVKTDINPGA
                     ETYAYNVMTRSGAAKIIANGADDLPLVDIDMKRYQSPIFTIAAGIRYSRQEIRQAQMMG
                     TSIDATKAEVARRTIAEKENSFIFVGDPKVNHKGVANAEGIQVINSPKKWKEMTSEEIV
                     EQLRTSRAKITIIPGFKGSSLKLMVAPEQYEELNRRYGEYDARSIMKVVQENGWFSSIE
                     QVYDLKGVGTDNSDSFIIMDTKPSTCEILLPEDIVRLEVEWSFPNWKVPFVERCGGALI
                     RTPYAIVRVDGI"
     gene            complement(5903..6373)
                     /locus_tag="PO880_RS08490"
                     /old_locus_tag="PO880_08490"
     CDS             complement(5903..6373)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377545.1"
                     /locus_tag="PO880_RS08490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_08490"
                     /product="hypothetical protein"
                     /protein_id="WP_113844215.1"
                     /transl_table=11
                     /translation="MTIPYPEKYMKPELGIGKLANYQGVQADSLVVGAGGLGFGVGVQV
                     TEDVATTYKDGQFYGISYAKNYVEEIPYGDVEKVGKYKEHEMVPILRKGSIWVKVDEDV
                     LAGENAKALSTGNFGKATISSDPATTPSDTVIGTFKTTASAGNLAVLQINLP"
     gene            complement(6376..7482)
                     /locus_tag="PO880_RS08495"
                     /old_locus_tag="PO880_08495"
     CDS             complement(6376..7482)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417184.1"
                     /locus_tag="PO880_RS08495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_08495"
                     /product="DUF2213 domain-containing protein"
                     /protein_id="WP_113844214.1"
                     /transl_table=11
                     /translation="MVIRYDKAFIQDFKETDEGYLTITACPITRPGVFPYRRTDGGLSM
                     EAKLPDELFSKTTVLSANAKPMTDDHPTEPVTAANYNKYSKGMTHNDAHVLDNKLLVSF
                     TITDAETIKKINDGKRELSIGFQADVSKETGVYNGMQYDSVQRNMQINHIAIVDEGRAG
                     PEVAIRGDSVAFMIDTKDKQTGGNGMSKLIIDSKEFEVDSIVKAKFESLEAKLDAAEQR
                     KANVEKLEGERDALKAQIDKLNKEIDEAKKKEVTADALDKRVQDRVDLINKAQKFLGDS
                     VDFTGKSDREIKESVIAKTSPDFKGDGKSDDYIAAYFDSAVANVEKKGFTNPAAFNDAK
                     DKDKDKEAAEEIEKQKNNRLNMNKKEDK"
     gene            complement(7495..7704)
                     /locus_tag="PO880_RS08500"
                     /old_locus_tag="PO880_08500"
     CDS             complement(7495..7704)
                     /GO_function="GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774203.1"
                     /locus_tag="PO880_RS08500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_08500"
                     /product="LysM domain-containing protein"
                     /protein_id="WP_002405646.1"
                     /transl_table=11
                     /translation="MAKEKKASSKEENKKELSKTTQKTIHTVSEGETASEIATRYHMSL
                     RKLLDLNELESINQVTEGIRLLVE"
     gene            complement(7697..7984)
                     /locus_tag="PO880_RS08505"
                     /old_locus_tag="PO880_08505"
     CDS             complement(7697..7984)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386748.1"
                     /locus_tag="PO880_RS08505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_08505"
                     /product="DUF6275 family protein"
                     /protein_id="WP_002386748.1"
                     /transl_table=11
                     /translation="MDSQKFIDLCKKHVVDFANSQLDYTDQKKISESDIYVVWLAKTLQ
                     NNKALLSTNLFDGMYYEVTFNGDKNELYFDAYKKWRNIRFDVTEGGDQNG"
     misc_feature    7997..8006
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     gene            complement(8041..8205)
                     /locus_tag="PO880_RS08510"
                     /old_locus_tag="PO880_08510"
     CDS             complement(8041..8205)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400412.1"
                     /locus_tag="PO880_RS08510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_08510"
                     /product="hypothetical protein"
                     /protein_id="WP_272731390.1"
                     /transl_table=11
                     /translation="MNKDEKNKRRWEDVPKSKSLGYPDEMTKKEIDTSKKEDKDFMQQL
                     KKSLKEKEE"
     gene            complement(8198..9442)
                     /locus_tag="PO880_RS08515"
                     /old_locus_tag="PO880_08515"
     CDS             complement(8198..9442)
                     /GO_component="GO:0044423 - virion component [Evidence
                     IEA]"
                     /GO_process="GO:0016032 - viral process [Evidence IEA]"
                     /GO_process="GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377552.1"
                     /locus_tag="PO880_RS08515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_08515"
                     /product="minor capsid protein"
                     /protein_id="WP_002405645.1"
                     /transl_table=11
                     /translation="MKNNPKTRYPLRLEESYAKNIQKAVKEIEKVSLYEFDKYLAPMID
                     DKNIVNDSNLINDGLFDAASKLIRIAQTYFLGILQNRTAQKIVRKHINSVNAFNKSNVN
                     SQLSARGINPLQTEKWLDSYVQAKIAENISYVTNIRDDYSKKFEQVIYRGITEGKSSNE
                     IREELVHQAGMSSDKAAFIARDQTGTILGQMNSERQKRAGFQAFRWSDSGDERVRDSHR
                     ERNGKIYFYADNPLLPGEEYNCRCVAEPVDDEELLEESIDLGLSNQEEHAVKTYVSSEA
                     YKLNDKLRNGYQLDESDLKLIDNLDKALDKMTNYDGEVTRSMFFDSSDDLVKFANNYNL
                     NDVVQFPEYISTTKDIYSEQDSLRFVIMSSTGKDLGSYNKSEKEVLFNRDAKFIVKDRY
                     LLDGKPYIVLEEYHE"
     gene            complement(9432..10826)
                     /locus_tag="PO880_RS08520"
                     /old_locus_tag="PO880_08520"
     CDS             complement(9432..10826)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406369.1"
                     /locus_tag="PO880_RS08520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_08520"
                     /product="phage portal protein"
                     /protein_id="WP_002405644.1"
                     /transl_table=11
                     /translation="MGNIANEAKLLKLDGKAYRSDFMLGNGKGHARDNLSRQRPGTSKR
                     LSHSQLESLYSSNSMAKNIVDIPAEDLTRNGWSLKMEDDKVKALYESKLRQLKAKERLQ
                     QLFTYERLYGDGFVSIGTIEKREYSLSEPLDFENIKSVPYINAFSGKKISNRIIDEDVF
                     SPNYGQIESFEVNNRSNNSRIHLLNNTTYSRATTIHRSRVLHQQNLRFEDELEGSSLLE
                     NLYDILTVADTSVWSVGQILYDFIFKVYKSSDVRNLTAEEKLEITMKMDYKFRTEALAI
                     IDSEESLGKESSSVAGIGELLDFVWDYLAGAARMPKTVLKGQEGGTVTGAQYDVMNYYS
                     RITAIQENQLRPHLEYLMRCLMWAEDECGGRLDPDSIEWSVEFNPLWNVDSKTDAEIRK
                     LTAETDKIYIEAGVSDPDDVHEARFGRFGITETSKFNADSLSPDEVDRMAAAVYANYKW
                     VRDHEK"
ORIGIN
        1 atttacacgg tcaattccta tatttttaaa tgaagcaaga tctcctgcca agtcaactaa
       61 tgaagtagac atttttgcag cttcttctgt gccaataccc attgaagttg acatatctcc
      121 atatgtagct gctaagtcta acgctgtacc ttgtgccaaa ccaatattag tcagtgtgga
      181 cttcgaccaa tcttccacaa ctttattatt atcaccaaaa gctacttcta ctttgtttaa
      241 agcttcatta gtatcagatg catagtcaaa ggcctttttg cctgctcctg cgattgctgc
      301 accagctatc attgcaccat ttcttatctt tgtaaaagca ttccaagcaa catctgaagc
      361 ctttgttgca acagtaccta tatggccaat acttgtttgt acacccttcg cagcttttcc
      421 aactactttt agagatgttg cactagattg agcgaaaaca ctcgcagaat ttttactttt
      481 atcaaactga tttccaacat cttttacttg tttttcagtt tgtttagcag agtttgttgt
      541 cttttgcata gaatcttttg ctcgatttcc gaactggaca actttatctg tattttgttt
      601 taaaccttca ctatttcttt ttaaagaggt tgtggcgcca tctatggatt ttgctgaatt
      661 ttgataagta ccttccattc gtgcagcttt agcaagaatt ttatcagttt cttcattggc
      721 acgctctaac gatctattat ttattttcca atcgagttcg attactgaac tacgtaacgc
      781 atctgccatt attagatgac acctcctctt gatttagtta gatttatttt ttgatatgct
      841 acttcgttcc aaactgctag ctcctctgcc gtggcaattt cgacttcatc tttagtggca
      901 atgcctgcta ttacaggcag ccacctaaac atatcttttt gaacttctct ttccgtaatt
      961 ctcggattag ggctagttaa gcattcgacc aagaaagttg tcggctaatg ccataacctc
     1021 acgatatcct tcgtgttcat cccaatagtc ccagttcaat tttggtgtaa caataactga
     1081 gtccataagt tgagagtggt aagcaacgtc agaaaaagta tttgatggtc cttttgagtt
     1141 atctaaaatc gcttgagctg cacgtgttcc agggaattga aatgtatatt ccacatcttc
     1201 aacagtatgt ttttcttgtt ttccaaactt actaaatggt tttttttctg cttctggaat
     1261 gttatttttc gcttctaact cttttacttc atcttttttc gtcatgataa attcctccta
     1321 aagttaaata aaaagcactt aacgagatgt taagtgccta gttatattcg tgtttgtaat
     1381 ctaatgcttt aatcgtataa gaacgagttg gaacgccttt accaaacgat ccatcaggcg
     1441 ttttttcaat ataggctttt gatgcccatg ctttttcagt tgaatgtgtc acagaaattg
     1501 caaattcttt acgaccatta gctaaagcca tcagctgttt gttacatggt gagttttgag
     1561 acaagttaat tgtaaaagtc cctaaattat cgttgttctt cgcagcacta gattgtcctt
     1621 gagcatctgt ttgtacttca atatacgaat tatcttttga aaaagatacc atgtcaccat
     1681 cttgaaagcc aaattggacg acattgtcaa taatagtaga tacctctttg gcatcataag
     1741 ttgtcatact ttgcattagt tagttccccc tttagacttc aattgttcca gtaacatcaa
     1801 cagtgtgaat tgctccagaa cgtttgtatt taaatgataa tcctttatag tttcgtgctg
     1861 caatgtcatc tggatttaaa tcttgacgtc ccaaagcagt cacactataa tttccaacac
     1921 cagtttcatc tacaatatct acaattccgt tattaaatgc agtttccaaa acatttgcaa
     1981 cggttgtatc taataaagca attccattag aatcaaaagt taatttatct gtcgttgata
     2041 acaagcgctg aacatttgtt tcaatgtttg atttaaccca gtgatcccca tgcagagcat
     2101 cgataaattc gccactcatt gtttttcctt ctgatgtttg ggggattccc gcttttgata
     2161 cataagcaat agcattagct ttctcaatcg cttgtagttg agaggtagtt aatgtgttag
     2221 gtgtaatccc tactaaatta tgtcggaatt tccaagtaac acttccgact gttaagcttg
     2281 cagtattacc aattaatgca gcatctaaaa attcttctaa cggatgaact aagccgatag
     2341 ttagattgtt ccctgtaaaa actgttaatt cgtcaactgt agctgtctga attactaaaa
     2401 acttaaattc gttttcttca attaaatttg atagtgccaa agcatctgcc tcgacaaagt
     2461 tggctaataa tgcaaaatgc caatcattat aaaagtaact tgtagctgcg gcgataattc
     2521 cacttccagc aattaagcta tctggctgtt ctggactagt agggttatct gctacaaaag
     2581 ttactactgc aatagtttgt ggtttatttt cctgtttcca aataatttct gcttttttat
     2641 aaacagtagt tgttgtagca aaatcttttg ctaatgtttc taaacttgta tattctttat
     2701 agttttgagt agatccttga acaaaaattg ctggatttcc taatccaaca atgggttgtg
     2761 gatgcattat gtcaattttt acattaacat ctgtaatttt ttcaatcatt tgttttcctc
     2821 ctgtatgtct atattttcta taatcacagc attgtcaacg tagctgtctt gtactctaaa
     2881 acgagcatca aagccagcca gacgttcata atcaatacta atgaaattat ctcgtttttg
     2941 agattgtgtt gttgaaacta aggctatttt agagttttga aggtctactt ttacagaaaa
     3001 accgtttaaa tgctttctta actgctctga caaattaagt gcctgaatac tagaatctgt
     3061 atgacattta atagacacta ctaattcaaa tacttcatta tcagtgacat ctatagtcac
     3121 tggaatatat ggcgagatga cctcataaga aaaaaacggt ttatctggct gtggaccagc
     3181 tgtaccacct tcgataagtt gacatcccgt acctttttgg acaacttcta tcagcttatt
     3241 tgccaacaaa ccatagttat aactttcagt catgaccctg caccgccttt aaaccatatt
     3301 gcataacatt cgaataatca gtatacggtg ttatagattc tactgaataa accctatttt
     3361 cttttaaaat tcgtgatttt ataggtactt gttcagatga gaaccaaatc aaatcatatt
     3421 cttcatatct accaccgtct ccatatttag cctgcattgg caattgtgtg atgagactac
     3481 ttggaattac aggatcgttc agttctctct tttcccatga ttcataagga gtttgaaacc
     3541 actctccatg aacataggtg ccttcttttt caggtttgac tagtagagta attggaatac
     3601 caaaactagc caaaacagaa gtgaagtcta gtttttgcat cagcctttca ccaccttata
     3661 tgttacagac tgccttaaac gccctgtatc tattaacgga ttacttgatt ttttattatg
     3721 aattgttatt ggtgaattag gtggatcaga taaatttcta atcgttcgct gaacatctcg
     3781 ctgaatgcgc aggcctaagt gattcattaa ttcattagca gtcaattctc catctactac
     3841 ttttttaact aacgtcaaag aataatctga ccactcttta tttttctcat caaaagtcga
     3901 acgcaagaat gaacgctctg gtattgtgat tttctcagtt aacatatatg caaactcaag
     3961 ctgttctttt cctttgttca ttactaaaaa cttatgccct tcttttgttt gcataaagaa
     4021 tagatcaaag tctctcggac tttttccttt atattttttt gatagaggaa tagttaagta
     4081 ttttccttta ggttttatcg tacaaccgaa ctcatggacg tttgcaatca tagccataaa
     4141 agaatcatcc gagccaaata ttccgatttg tataacaaat cgttttagca aggataattc
     4201 tttaataatt tgaggaattc tgtttatttc atttattttc attaaatcac taccaaactc
     4261 aatgaacctt ttttagcgta ttcgttgtaa agacgtaaat actcttggcc ataaacggtt
     4321 ctttttaggt cggtaaaagt tgagtgaaaa cccgaatact ctttttttag cgagcctact
     4381 tgctcggatt tagtattttg gttgttcaaa acagctagat ggcaagcgag atatcgacac
     4441 gctttctcct taacatcttc tttaaatggc aatgcatcta cttctagcca agcatcatca
     4501 ataaacaact taatagaatc attgttaact cctgctagtt ctgcagctgt taacctaaca
     4561 ttttcaactg tgctttttgg catagtacca cttcctattc tggattaact acgctgtctt
     4621 catcagggtt tttaatatca tcaatttgat tttcgattgc tttgactaca gttgtgcgat
     4681 ttttgtttgc ttgctcttct tccaaccatt tttccaacaa ttctaaatcg aacgtatcag
     4741 caatggattc tactgcttta tttgctgtta attcagtaaa accaactgct ttttttgatt
     4801 ttccttttgt tatatggtcc aaaatctcaa tttctcctaa tctttccaac gatttattta
     4861 atggtaattc cataccttta ataaattctt cagcatctga attatctaaa tcatttacac
     4921 ctggaattaa tcgaacattt ccaatatgtc taatatatgc acctttattg tgtactaaca
     4981 taatctttac ctccttattt ttaaatacca tccacacgaa caattgcata tggcgttcta
     5041 attaacgcac caccacaacg ttctacaaat ggtactttcc aatttggaaa agaccattca
     5101 acctctaaac gcacgatgtc ttctggaagt aaaatttcac aagttgatgg ttttgtatcc
     5161 atgataataa atgaatcaga attatcagta cctacacctt ttaaatcata aacttgctca
     5221 atagatgaaa accagccatt ttcctgaaca actttcataa ttgatcgggc atcatattca
     5281 ccataacgac gattcaattc ttcatattgt tctggagcaa ccattaattt taaactagat
     5341 cctttaaatc ctggaataat agtaatttta gctcgagatg tacgtaattg ttcaacaatc
     5401 tcttcactgg tcatttcttt ccacttctta ggtgaattaa taacttgaat accttcagca
     5461 ttcgcaacac ctttatggtt tactttagga tctcccacaa aaataaagct attttctttt
     5521 tcagcaatag tacgtcgtgc cacttctgct tttgttgcat caattgaagt tcccatcatt
     5581 tgagcttgac gaatttcttg tcgactataa cgaataccag cagcaattgt aaaaattggt
     5641 gattgataac gtttcatatc aatgtcaact aaaggaagat catctgcacc atttgcgata
     5701 atttttgcag cgccacttct agtcataaca ttatatgcgt atgtttccgc tcctggattg
     5761 atatctgttt taacattaaa catggttcgc gccacaagtt cctcctgtgg tgcttgataa
     5821 atgaccttat caatttcttg taggtcacgt gcttctaaag ttgctgttac atcatttccc
     5881 atttagttgt cctcctagtt ttttaaggta agttaatttg taaaactgct aaattaccag
     5941 ctgatgctgt tgttttaaat gtaccaatca cggtatctga tggtgtcgta gctggatcag
     6001 aactaatagt tgcttttcca aaatttccag ttgataaagc ttttgcattt tcacctgcta
     6061 aaacatcttc gtcaactttc acccaaattg accctttacg taagatcggt accatttcgt
     6121 gttctttata tttaccaact ttttccacat ctccgtaagg gatttcttct acataatttt
     6181 tagcatatga aataccataa aattgcccat ctttatatgt ggtagcaacg tcttctgtta
     6241 cttgcacacc gacaccgaaa cctaagccac cagcacctac aacaagacta tctgcttgta
     6301 caccttgata attagctaat ttcccaatac caagttcagg cttcatatac ttttctggat
     6361 aaggaatagt catagttatt tatcctcctt tttgttcatg ttcaaacggt tatttttttg
     6421 tttttcaatt tcttccgccg cttctttatc tttatcttta tcttttgcat cgttgaacgc
     6481 tgctggattt gtaaatcctt ttttctccac atttgcaact gctgaatcaa agtatgctgc
     6541 aatataatcg tcagattttc catcaccttt aaagtcaggt gatgttttgg caattactga
     6601 ttctttaatt tcacgatcag atttgccagt gaaatcaact gaatcaccaa gaaacttttg
     6661 tgctttatta attagatcaa cacgatcttg aacacgctta tccaaagcat ctgcagttac
     6721 ttcttttttc tttgcttcgt caatttcctt attcaactta tcaatttgag cttttaaagc
     6781 atctcgctca ccttctaatt tctcaacgtt tgctttgcgt tgttcggctg catctaattt
     6841 tgcttctaaa gattcaaatt ttgcttttac aattgaatcc acttcaaatt cctttgaatc
     6901 aataattaat ttagacattc catttcctcc tgtttgttta tctttagtat caatcatgaa
     6961 agcgactgaa tctccccgaa tagcaacttc gggaccagct ctcccctcat ctactatagc
     7021 aatatgatta atttgcatgt ttctctgaac agaatcatat tgcatgccat tatacacgcc
     7081 agtttctttt gaaacatctg cttgaaagcc gatgctcagt tctcgttttc catcgttaat
     7141 tttttttatt gtctctgcat ctgtaattgt gaacgaaacc aatagcttat tatctaaaac
     7201 gtgagcatca ttatgagtca tgccttttga atatttattg taattagctg ctgttactgg
     7261 ttcggtcgga tgatcatcag tcattggctt agcatttgct gaaagtacag ttgtcttaga
     7321 aaataattca tcaggcaatt ttgcctccat tgataatccg ccatcagttc gacggtaagg
     7381 gaaaacacct ggtcgtgtaa ttggacaagc tgtgattgtc aaatagcctt catcagtttc
     7441 tttaaaatcc tgaataaagg ctttgtcata tctaattacc atgtcttcac cccactattc
     7501 gactaataag cgaatgcctt cggttacttg attaatcgac tctaactcat taagatccag
     7561 taattttcgt aggctcatat gatagcgtgt cgcaatttca ctagcagttt ctccctcaga
     7621 aacagtatga atcgttttct gcgtagtttt tgacaactct tttttgtttt cttctttact
     7681 agaagctttt ttctctttag ccattttgat cacctccttc agttacatca aacctgatat
     7741 ttctccactt tttataagca tcgaaatata gttcattttt atcaccgttg aaggtaactt
     7801 cgtaatacat accatcaaat agattggtac ttaataatgc tttattattt tgcagggttt
     7861 tcgctaacca gaccacgtaa atatcagact cgcttatttt tttttgatct gtgtaatcca
     7921 attgactgtt agcaaaatca actacatgct ttttacataa atcaataaat ttttgactat
     7981 ccattaatgt ccctcctgtt ttttgagtat aaaaaatagc cccaacctat aaggtcggtg
     8041 ctactcttct ttttctttta aagatttctt caattgctgc atgaaatctt tatcttcctt
     8101 tttcgatgta tctatttctt tttttgtcat ctcatctggg taacctagac tttttgattt
     8161 gggaacgtct tcccatctac gtttgttttt ctcatcttta ttcatggtac tcctccagta
     8221 ctatatatgg ttttccatcc aataaatatc tgtctttaac aataaacttg gcatcacgat
     8281 taaacagaac ttctttttca gacttgttgt aagaacctaa atcttttcca gttgagctca
     8341 ttataacaaa tcttaacgag tcttgctctg agtaaatgtc ttttgtagtc gaaatgtatt
     8401 caggaaattg aacaacatca tttaaattgt agttgttagc aaacttcact agatcatcac
     8461 tgctatcaaa aaacatggaa cgtgttactt caccatcata gttggtcatt ttgtctaacg
     8521 ctttgtctaa attgtctatc aatttcaagt cgctttcatc taactgataa ccatttctta
     8581 gcttatcgtt taatttgtaa gcttcagagc taacatatgt cttgaccgca tgctcttctt
     8641 gattagaaag gccaagatca atgctttctt caagcaattc ttcatcgtcg actggttcag
     8701 caacacagcg acaattatat tcttcgcctg gtaataatgg attatcagca taaaagtaaa
     8761 tcttcccgtt acgttctcga tgagaatccc taactcgttc atctccacta tcactccatc
     8821 taaaagcttg aaatcccgct cgtttttggc gttcactatt catctgacct aaaatagtac
     8881 ctgtctgatc acgagcaata aacgctgctt tgtctgatga cataccagct tgatgaacaa
     8941 gctcttctct tatctcgttt gaagatttgc cttctgtgat tccgcgataa ataacctgtt
     9001 caaacttttt tgagtaatca tcacgaatat tagtgacata actgatgttt tccgctattt
     9061 tagcttgaac atagctgtct aaccattttt cagtctgtag tgggtttatt cctctagcgc
     9121 taagttgaga gttcacatta gacttgttaa atgcgttgac actattaata tgcttacgaa
     9181 ctattttttg tgcggttctg ttttgaagaa tacctaaaaa gtatgtttgc gcaattctga
     9241 ttagtttcga tgcggcatcg aataatccat cattaattaa gttggaatca ttaacaatat
     9301 ttttatcatc tatcatcggt gctagatact tatcaaattc atataatgaa actttttcta
     9361 tttcttttac ggccttctga atgtttttag catagctttc ttctaaacgt aacggatatc
     9421 tcgtttttgg attatttttc atgatccctt acccatttat agttcgcata gacagcagca
     9481 gccattctgt ctacttcgtc tggtgataag ctatctgcgt taaatttaga agtttcggtt
     9541 atgccaaacc gaccaaatct tgcttcatgg acatcatcag gatcagaaac gcctgcctcg
     9601 atgtatattt tgtctgtctc agcagtaagt ttccttattt cagcatcagt tttgctgtcc
     9661 acattccaaa gtggattgaa ttcaacggac cattcgattg aatcagggtc aagtcgacca
     9721 ccacattcgt cctctgccca cattagacac cgcatgagat attcaaggtg tggtcttaac
     9781 tgattttctt gtatagcagt tatacgagaa tagtagttca tgacatcata ttgtgctcca
     9841 gtaacagttc caccctcttg accttttaaa acagtcttag gcattcgagc agcccctgct
     9901 aagtaatccc aaacaaagtc taaaagttca ccgatacctg ctaccgaaga actttcttta
     9961 ccaagacttt cttccgagtc aataatagca agcgcctctg ttctgaactt gtaatccatt
    10021 ttcattgtaa tttctaattt ttcttctgct gttaaattac ggacatctga tgacttgtat
    10081 actttgaaaa taaaatcata cagaatttgt ccgactgacc aaacagatgt gtcagctaca
    10141 gttaagatat cataaagatt ctctaataaa gatgatcctt ctaattcatc ttcaaatctc
    10201 aaattttgtt gatgtaaaac tcgtgaccta tgaattgttg tagctctgct gtaagtcgta
    10261 ttatttagaa ggtgtatgcg actgttgttt gatctattat tgacttcaaa agattcaatc
    10321 tgtccatagt ttggactaaa aacatcttca tcaataattc tattactaat ctttttgcct
    10381 gaaaatgcat taatatatgg aacactttta atattttcaa agtctaatgg ctcacttaga
    10441 gagtactctc ttttctctat tgttccaata cttacgaagc catctccata taatcgttca
    10501 tacgtgaata attgttgtaa tcgttcctta gccttcagtt gtctcagctt actttcgtac
    10561 agagctttta ctttatcatc ttccattttg agactccatc catttctagt taagtcttct
    10621 gctggaatgt ctacgatatt ttttgccatc gagttagacg agtaaagcga ctctaattgt
    10681 gagtgtgaca atctcttgct cgtacctggt ctttgtctag ataagttgtc tctagcgtgg
    10741 cctttgccat ttccaagcat aaagtcactt cgatatgctt taccatccaa ctttaataac
    10801 ttagcttcat tagctatatt ccccatttac tcaccgcctt tcaatttttt aattgtcgag
    10861 atacctttct cttaaactta ct
//
