LOCUS       NZ_CP116962            24382 bp    DNA     circular CON 10-MAR-2024
DEFINITION  Enterococcus faecalis strain DM86 chromosome, complete genome.
ACCESSION   NZ_CP116962
VERSION     NZ_CP116962.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2959291)
  AUTHORS   Shen,W. and Cai,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) Clinical Microbiology Laboratory, The Second
            Affiliated Hospital of Zhejiang University School of Medicine,
            Zhejiang University, 88 Jiefang Road, Hangzhou 310009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/10/2024 16:14:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 29 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 6 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:47:18
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 995062
            Orig. end                         :: 1019444
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116962.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..24382
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lassopeptide"
                     /region_number="2"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..24382
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..24382
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:14382](-)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..14382)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     misc_feature    complement(165..178)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 20.8"
                     /tool="antismash"
     gene            578..1135
                     /gene="frr"
                     /locus_tag="PO880_RS04680"
                     /old_locus_tag="PO880_04680"
     CDS             578..1135
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="frr"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /locus_tag="PO880_RS04680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_04680"
                     /product="ribosome recycling factor"
                     /protein_id="WP_002356762.1"
                     /transl_table=11
                     /translation="MSKEVLATAKEKMTKAEESLRRELGQIRAGRANASLLDRIQVEYY
                     GAPTPVNQLASINIPEARVLMITPFDKNSIADIEKAIQMSDIGISPTNDGNVIRLVIPQ
                     LTEERRKELAKDVKKEAENSKVAVRNVRRDAMDELKKAQKNGDITEDELRSFEKDVQKL
                     TDDSIKNIDAITAEKEQELLEV"
     misc_feature    1258..1269
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: strong, score: 19.78"
                     /tool="antismash"
     misc_feature    complement(1429..1447)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: medium, score: 28.04"
                     /tool="antismash"
     misc_feature    complement(1430..1446)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.37"
                     /tool="antismash"
     gene            1505..2275
                     /gene="sufC"
                     /locus_tag="PO880_RS04685"
                     /old_locus_tag="PO880_04685"
     CDS             1505..2275
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sufC"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317772.1"
                     /locus_tag="PO880_RS04685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_04685"
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="WP_002356763.1"
                     /transl_table=11
                     /translation="MSVLEIKNLHVSIEDKKILKGVDLTINTGEIHAIMGPNGTGKSTL
                     SAAIMGNPNYEVTEGEILFDGVNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMRAA
                     INAKRDEDNKMSVMQFLKKLDSKMELLNMPEEMAERYLNEGFSGGEKKRNEILQLLMLE
                     PTFAILDEIDSGLDIDALKVVSKGVNEMRGENFGALIITHYQRLLNYITPDVVHIMMEG
                     RVVKTGGADLAKRLETEGYAGISQELGIDYKEEA"
     gene            2290..3576
                     /gene="sufD"
                     /locus_tag="PO880_RS04690"
                     /old_locus_tag="PO880_04690"
     CDS             2290..3576
                     /GO_function="GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /GO_process="GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sufD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634218.1"
                     /locus_tag="PO880_RS04690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_04690"
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="WP_002364433.1"
                     /transl_table=11
                     /translation="MKKVNLMDYLDEVTAFSLEHEEPAWMTELRTTALRNADESELPHI
                     DRVKFHRWPLLNVHMESYVPSEGNVASFDQMKDNPLIVQQGSFHAFEQLPASLAEQGVI
                     FTDIFTALQEHPELVKEYYMTKAVLPEEDKLTAAHAAFMNSGVFLYVPKNVVIEEPIES
                     LFIQDSDSNQPFFKHVLIVADNHSEFSYLERFQSTGHHAEKASGNIVVEVIAKDGAKVK
                     YSAVDQLGQTVTTYMNRRGYIMRDASVDWALGVMNDGDVVADFDSDLVGEGAHSEVKVV
                     AISAGKQTQGIDTRVTNKAPHSIGHILQHGVIRERGTLTFNGIGHILKGAKGADAQQES
                     RVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLRKEEAERLVIR
                     GFLGSVITAIPVKEVQNEFVEVIEGKLNA"
     gene            3573..4808
                     /locus_tag="PO880_RS04695"
                     /old_locus_tag="PO880_04695"
     CDS             3573..4808
                     /EC_number="2.8.1.7"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_5 (23-395). E-value: 4.3e-162.
                     Score: 531.2. Matches aSDomain:
                     nrpspksdomains_PO880_RS04695_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010712667.1"
                     /locus_tag="PO880_RS04695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_04695"
                     /product="cysteine desulfurase"
                     /protein_id="WP_002400835.1"
                     /sec_met_domain="Aminotran_5 (E-value: 9.5e-162, bitscore:
                     535.5, seeds: 34, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_1_2 (E-value: 3.8e-10, bitscore:
                     36.8, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 2.6e-09,
                     bitscore: 34.1, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMDAATIRQSFPILFQEVNDEPLVYLDNAATTQKPTAVLDVLRHY
                     YETDNANVHRGVHTLAERATKDYEASREKVRQFIHAKETAEVLFTRGTTTSLNWIAKSY
                     GDLAVTAGDEIVISYMEHHSNIIPWQQLAKRTGAILKYIDVTEDGFLDMASARQQITEK
                     TKIVSIAHVSNVLGVINPIEELTQLAHQNGAVMVVDGAQAVPHMPVDVQAIDADFYAFS
                     GHKMCGPTGIGVLYGKRHLLEQMEPVEFGGEMIDFVHLQESTWKELPWKFEAGTPNIAG
                     AIALGAAIDYLTEIGLEAIHQHEAALVHYVLPKLQAIEGLTIYGPQDPKDHTGVIAFNI
                     EGLHPHDVATALDMEGVAVRAGHHCAQPLLNYLSVPATARVSFYLYNTKEDADRLVEAI
                     KATKEFFQHGTF"
     aSDomain        3642..4757
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO880_RS04695_Aminotran_5.1"
                     /evalue="4.30E-162"
                     /label="PO880_RS04695_Aminotran_5.1"
                     /locus_tag="PO880_RS04695"
                     /protein_end="395"
                     /protein_start="23"
                     /score="531.2"
                     /tool="antismash"
                     /translation="VYLDNAATTQKPTAVLDVLRHYYETDNANVHRGVHTLAERATKDY
                     EASREKVRQFIHAKETAEVLFTRGTTTSLNWIAKSYGDLAVTAGDEIVISYMEHHSNII
                     PWQQLAKRTGAILKYIDVTEDGFLDMASARQQITEKTKIVSIAHVSNVLGVINPIEELT
                     QLAHQNGAVMVVDGAQAVPHMPVDVQAIDADFYAFSGHKMCGPTGIGVLYGKRHLLEQM
                     EPVEFGGEMIDFVHLQESTWKELPWKFEAGTPNIAGAIALGAAIDYLTEIGLEAIHQHE
                     AALVHYVLPKLQAIEGLTIYGPQDPKDHTGVIAFNIEGLHPHDVATALDMEGVAVRAGH
                     HCAQPLLNYLSVPATARVSFYLYNTKEDADRL"
     gene            4795..5262
                     /locus_tag="PO880_RS04700"
                     /old_locus_tag="PO880_04700"
     CDS             4795..5262
                     /GO_function="GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /GO_process="GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362181.1"
                     /locus_tag="PO880_RS04700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_04700"
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="WP_002356768.1"
                     /transl_table=11
                     /translation="MALSKLDNLYRQVILDHSSHPHHHGTLDASSQTIELNNPTCGDVI
                     ELDVAIEDGVIKDIAFQGSGCSISTASASMMTDAVLGKTIAEATALAEDFSQLVQGNEV
                     AEDEKLGDAAMLSGVAKFPARIKCATLAWKALEQAVANNGQGEAGHLHCEK"
     gene            5281..6675
                     /gene="sufB"
                     /locus_tag="PO880_RS04705"
                     /old_locus_tag="PO880_04705"
     CDS             5281..6675
                     /GO_function="GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /GO_process="GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sufB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356769.1"
                     /locus_tag="PO880_RS04705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_04705"
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="WP_002356769.1"
                     /transl_table=11
                     /translation="MSTVPELEEYKFGFHDDVEPVFSTGDGLTEDVVREISRVKGEPEW
                     MLDFRLKSLEQFNKMPMQEWGPDLSDIDFSKIKYYQKPSDKPARDWDDVPDKIKETFEK
                     IGIPEAERAYLAGASAQYESEVVYHNMKEEFEKLGIIFTDTDSALKEYPDLFKEYFSKL
                     VPPTDNKLAALNSAVWSGGTFIYVPKGVRVDVPLQTYFRINAENTGQFERTLIIVDEGA
                     SVHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKAYEGA
                     TVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAPNTSSS
                     IVSKSIAKDGGEVNYRGQVTFGKDSAGSISHIECDTIIMDEKSKSDTIPFNEIHNSQVS
                     LEHEAKVSKISEEQLYYLMSRGLSEAEATEMIVMGFVEPFTKELPMEYAVELNRLISYE
                     MEGSVG"
     gene            complement(6761..>6898)
                     /locus_tag="PO880_RS15380"
                     /pseudo=""
     CDS             complement(6761..>6898)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367047.1"
                     /locus_tag="PO880_RS15380"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="site-specific integrase"
                     /pseudo=""
                     /transl_table=11
                     /translation="EDIMYRVGHKDSKMTRQVYNYFYPERKERSADQFAQFIEKEKYLF
                     "
     gene            complement(7726..8454)
                     /locus_tag="PO880_RS04710"
                     /old_locus_tag="PO880_04710"
     CDS             complement(7726..8454)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634216.1"
                     /locus_tag="PO880_RS04710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_04710"
                     /product="ABC transporter permease"
                     /protein_id="WP_002401908.1"
                     /transl_table=11
                     /translation="MVKNILQSEIIKFFSYNWCLFGTVGTILVAPVILFFSGIGNDQPL
                     VTNEILSMSIRNFFLSQAGLVIIAASFFGQEYSNSYLRTTLLTIPARIKLITTKLFLLT
                     VIVWIIGLFSSLICLGIGIIQFNGSFTLSLILEFLIKVIIAMLSWTQITWITVFLTIIT
                     KSHILPIAIMFSLILGLSQMLFAMTAFAKYLPDLATMNLFFSTHTTTLLNGSQGLLVQS
                     IWSLFLSLISFGLIQYRDVR"
     gene            complement(8454..9239)
                     /locus_tag="PO880_RS04715"
                     /old_locus_tag="PO880_04715"
     CDS             complement(8454..9239)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362855.1"
                     /locus_tag="PO880_RS04715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_04715"
                     /product="ABC transporter permease"
                     /protein_id="WP_002362855.1"
                     /transl_table=11
                     /translation="MSFVKVTYSEFSKLINSRGWWIVFGLVIVLQPLLGLLEADQLLSI
                     GLDATPATHPDLLEAMPPLDYFGFDAALFGVLPIVIWGGISGANEYKNHNLRTTMLYSN
                     KRKELFNAKLLTILISTISISFISIFITVAITHVRLGSLGLNPFILSQTTWKFIGYTTL
                     NWILLTVLSFLIGLLFRNMIVPLIFLVPQIYNLGSYLAEKWPWGEYLPVAAGNFIVATP
                     VKSYPHDPAKGTLILLLWIFFSMLIAFYSFTRSDVGGKY"
     gene            complement(9242..9949)
                     /locus_tag="PO880_RS04720"
                     /old_locus_tag="PO880_04720"
     CDS             complement(9242..9949)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378542.1"
                     /locus_tag="PO880_RS04720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_04720"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_002378542.1"
                     /transl_table=11
                     /translation="MKNIVLSNICKQHKKKNILENITFKAKEGRITAFLGPNGAGKSST
                     LRILLGLDLPLSGTATFGKKKYSNFIFPLQIVGSAFDGIGGTPSRKVKTHLKIIAKSNN
                     IPNKRISEVLDLVDLSNKSHAQLGTLSLGEGQRLGIAIALLGDPQYLVLDEPTNGLDPM
                     GIKWFRNFIRRQADQGKTVLLSSHYLSEIEAIADDVVIINKGQIVVTGELHEVMQNIET
                     LEDVFFLLTEGGN"
     gene            complement(10001..12292)
                     /locus_tag="PO880_RS04725"
                     /old_locus_tag="PO880_04725"
     CDS             complement(10001..12292)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401909.1"
                     /locus_tag="PO880_RS04725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_04725"
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_202492343.1"
                     /sec_met_domain="PF13471 (E-value: 2.3e-21, bitscore: 73.4,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIQLFPEQIDPVSFSARFLARLSLILSAPLNWMSPGRIEKILVK
                     LIKNNPPASEKQVKIARNAVCIVSSRCRSQEGCLRRSLAVVIVLWLQRKSVSWCTGYAQ
                     EPFRAHAWIELNECPIGEPVEVQIYSKAICVPHRSNDILRKESYLKKIKQENIERKKKE
                     SEIVNPEDHTPSVNIRSLFALAKGHNWEFICVGILGLISAALTLAQPNLVANLIDQSNT
                     GIKINSSLGILIVVLILSTILTAVQYYLLQIIGEGVVFKARKSLISHLLRLPIHEYDTR
                     SVGDLLSRVSGDSSKLRMALIQVSIALSSGFLVVVGAAIGMILRDSLLFFMALSTVCVS
                     FIGTIIMSKAIQKASFSAQKELGKLSGDIERDLHAIRTIRATNATDKEEEKSKLQAERL
                     RNIGIRLAKIQAFMTPISNMTLQLSGLIVLGIGGYRVSIGLMSVADLIAFALLLYTMIG
                     PIGQVFNAFGGVGESLGAFARIRELLQLALESDYDVTPAIPPSSNLITEETAVYFEKIS
                     FGYNKMKFGSDPKEKEDSMILKEVTLRAEKGKRTAIVGPSGAGKSTLLQLIERFYDPDS
                     GKIFVQGKDYRHYSREELRNFITYVEQDAPVISGTLRDNLLLGNSKISDSNCIEVLEEV
                     NLHHLLDRNSKGLDMQVGESGANLSGGERQRLAMARSLLSSAEIVLLDELTSNLDSLNE
                     LGMKKAVDKLKGKKTVIVVAHRLSTVIDSDIIYVLEHGRVVGSGNHNELIDTVPLYREL
                     AKEQFVDREL"
     gene            complement(12292..12540)
                     /locus_tag="PO880_RS04730"
                     /old_locus_tag="PO880_04730"
     CDS             complement(12292..12540)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362859.1"
                     /locus_tag="PO880_RS04730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_04730"
                     /product="PqqD family protein"
                     /protein_id="WP_002362859.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 5.3e-22,
                     bitscore: 75.1, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 5.6e-09, bitscore: 33.6,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKVKRELKIISQDDGAIVLDKSRGIYFQVNASGVSMLEGLSKGL
                     TIEELALQLETSFSLDKAQAETDVAEFMALLKEMKLA"
     aSDomain        complement(12295..12540)
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_PO880_RS04730_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="5.30E-24"
                     /identifier="RREFam002.1"
                     /label="PO880_RS04730"
                     /locus_tag="PO880_RS04730"
                     /protein_end="82"
                     /protein_start="0"
                     /score="75.1"
                     /tool="antismash"
                     /translation="MRKVKRELKIISQDDGAIVLDKSRGIYFQVNASGVSMLEGLSKGL
                     TIEELALQLETSFSLDKAQAETDVAEFMALLKEMKLA"
     gene            complement(12598..14382)
                     /locus_tag="PO880_RS04735"
                     /old_locus_tag="PO880_04735"
     CDS             complement(12598..14382)
                     /GO_function="GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA]"
                     /GO_process="GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634142.1"
                     /locus_tag="PO880_RS04735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_04735"
                     /product="asparagine synthase-related protein"
                     /protein_id="WP_224567137.1"
                     /sec_met_domain="Asn_synthase (E-value: 9.3e-15, bitscore:
                     52.5, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLLIIVNKSNISISSKKLMFQKRITTDYTNILLVSNTEYSQHTHD
                     QFEIYCISSEYSRIFCNKDQFNYTTTENMLDYLQANISKGVFVLINKEGLFMGGSLFDV
                     SLSWLDSNDELIISDSSANIALFLNLPISKSALALNLIHNLPYYPFQNIPLWEKVNTIQ
                     PFYILKCTTNGILEPIQTWTPPNLENKVESIYSKIRERLLELLSQNTVSYSEISADLSG
                     GVDSATIIYILKALQANVKLYHANADSEWNSDSTWAKLISNDINVIFTSLPSLGSSGKN
                     FEIDRDYSNSVLPDSPLFWADTEGYVESIEHSALTPANATHFTGLGGDELFTPMPANAW
                     SLVRQNKLRSIDLGLRYCLLNRIPISVGTVDLINNNSFKEAVKNEIDKGFGYAKPTRQN
                     SALNWCGPVTVPNWLTKPYRELSYKVLINKLDHTSNSLDSDRSRHQMIESLLFQRHLVT
                     QINNIYGVNGMTWQAPFLDVDIINYSLAIPARYRQDHTVTKSILYNATKGLVSKNIFTR
                     GFKGDYSTSLYKSYKEAVKKHSQQVHQFKLVELGIVDADILISELSMPSALHTRVEAFE
                     RLAAVERWLRVALKKNSK"
     gene            complement(14503..14640)
                     /locus_tag="PO880_RS04740"
                     /old_locus_tag="PO880_04740"
     CDS             complement(14503..14640)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414657.1"
                     /locus_tag="PO880_RS04740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_04740"
                     /product="hypothetical protein"
                     /protein_id="WP_002414657.1"
                     /transl_table=11
                     /translation="MTKQNYSKPSIEKIAGFKESTRGLWTGKFRDVFGGRALFQVVIYY
                     "
     gene            15100..17061
                     /locus_tag="PO880_RS04745"
                     /old_locus_tag="PO880_04745"
     CDS             15100..17061
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002410798.1"
                     /locus_tag="PO880_RS04745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_04745"
                     /product="ATP-binding protein"
                     /protein_id="WP_202492342.1"
                     /transl_table=11
                     /translation="MKEKFVYLLSLFVFLVIALLTIKQNDYLYGSQAVTVVPIIVVLIL
                     FCAVIIFVSKDIKKIRYFAVLTFFTSISLAIGLFPYSFVHPVKSLLISFLPYFVYKFFV
                     TFNLISILSVSKKIDQILFIQSIIVSMLLLLNFEKFIWMVSIVIILDIFGCLFLYFKGK
                     DRLTIRTKKEQVFLIRILVLSLFSLIISTSILSGLVLFLIPITVGIILIKRNEISLDKV
                     SIIINLVIHIICIGIFFIINFYFLRLSILDIYFISIFYCLILFILYLKNWYLSSIEVSR
                     IQQRKKDFQKEKIDFVQTISKEKTLSSVSTLISTIFLQHYEINSYLMVWKDQYQPYILS
                     SSGEFSKLILTNKIIAEFERDSNIFHYHNNKYRKFTFFENKQILGWLLIGREKDFDLAS
                     MKHEEIYDLVGVAGKIIIENERLYDIKQESLKIESLSYDEYVNYEYLNMVQNFHKDFSF
                     FIHDNVLQNILALKKLIESMSTENVETKKLVLETFDTLNKTFRDKIFELYPSTIETVPV
                     SQSIRILCDKFNEGQESIFVHFYCPQEMHLKKEEKFHIYRIIQELITNALKHSRATVVC
                     VYLIQRDFYIECIVQDDGTGFDYSKIKLRGFENKHFGILSINQEVNSLNGKMRVSSVNP
                     HGTKFTITLPIAKEDIDS"
     gene            17058..17651
                     /locus_tag="PO880_RS04750"
                     /old_locus_tag="PO880_04750"
     CDS             17058..17651
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362865.1"
                     /locus_tag="PO880_RS04750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_04750"
                     /product="response regulator transcription factor"
                     /protein_id="WP_002362865.1"
                     /transl_table=11
                     /translation="MKVYLLDDHALFSKSLEIAFQPMDVSITSFTNPEFFLNKLKNEQP
                     DLALLDIHIGELSGFEISKEIIGKFPNQKIVFLSGFDLIEYKNEAIKSGAWGLLNKNVT
                     IEELYDDLQKIHHGTDLLPKCQSTLDVLSKREKEILKCAAEGFKQQEIAEQLHISRRTV
                     NNHLVSINNKLEVNSTISAIIQAIELGIIRVKGY"
     gene            complement(17702..>18273)
                     /locus_tag="PO880_RS04755"
                     /old_locus_tag="PO880_04755"
                     /pseudo=""
     CDS             complement(17702..>18273)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002415745.1"
                     /locus_tag="PO880_RS04755"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="PO880_04755"
                     /product="CHAP domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="IQFSKTQADGKTVTYTNPIAINIGYDYRYAYGNMFYAQIVKQYIT
                     SISNNNSQGNSAIVKAALKELNEGIHKGGTKYWQWYGFNGRVEWCAIFVSYNAEKAGVK
                     MEHFAYCPTGIENFKANNQWFRKGSLPKNGNIIFFDWDGDSISYYVGIVEKVENEVVYT
                     IEENSGNKIAKLSYEKIALISWVMVFR"
     gene            complement(<18278..>18466)
                     /locus_tag="PO880_RS15385"
                     /pseudo=""
     CDS             complement(<18278..18466)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392750.1"
                     /locus_tag="PO880_RS15385"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="ATP-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MDITDKKANSPHIAITGPTVNGKSYLAKFIFFLSVLMNGKGLYVD
                     PKSEFFNRIMEVINHPLY"
     misc_feature    18701..18714
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.62"
                     /tool="antismash"
     gene            complement(19162..19764)
                     /locus_tag="PO880_RS04765"
                     /old_locus_tag="PO880_04765"
     CDS             complement(19162..19764)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002413059.1"
                     /locus_tag="PO880_RS04765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_04765"
                     /product="hypothetical protein"
                     /protein_id="WP_202496847.1"
                     /transl_table=11
                     /translation="MKLVKNVLLILIGLVIIGIGASTLRVAGLGVDPFTAMNIGLSSMF
                     GMQLGIFQIIVNAFILFYVYLKNKKAIGLGTILNMLLVGILIQQISTWLQPYANSLGGS
                     TIGKLLSLFVGVLIFTFGCALYMATDQGESPYDAIAPTICQQINKPYASVRKAQDLLVL
                     LIGFIAGGPIGIGTIICAFGADPLISFWNKLLQPLVK"
     gene            complement(19850..20629)
                     /locus_tag="PO880_RS04770"
                     /old_locus_tag="PO880_04770"
     CDS             complement(19850..20629)
                     /NRPS_PKS="Domain: PKS_KR (50-167). E-value: 2.8e-07.
                     Score: 22.5. Matches aSDomain:
                     nrpspksdomains_PO880_RS04770_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376895.1"
                     /locus_tag="PO880_RS04770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_04770"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_010710611.1"
                     /sec_met_domain="adh_short (E-value: 6.7e-30, bitscore:
                     101.8, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 8.9e-31, bitscore:
                     105.1, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYGELHGKVAVVTGAATGLGLFITLRYILEGMNVVADYVGELPKE
                     FEDVQAKHADRVKFVKADVSNEEDIKALAETALKEFGHVDIWVNNAGVEASFPTIDMPL
                     KEWQRVINVNLNGVFLGSREALRIFRDQKIKGSIINMSSVHQRIPWPTFAHYAASKGAT
                     EMFTKTIALEYAEYGIRANCIAPGAINTPINAEKFSDPEQLKQTTSMVPMGIIGKPNQV
                     AAAAAWLASDESSYVTGTTLFVDGGMSLYPSFQHGAG"
     aSDomain        complement(20129..20479)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO880_RS04770_PKS_KR.1"
                     /evalue="2.80E-07"
                     /label="PO880_RS04770_PKS_KR.1"
                     /locus_tag="PO880_RS04770"
                     /protein_end="167"
                     /protein_start="50"
                     /score="22.5"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="AKHADRVKFVKADVSNEEDIKALAETALKEFGHVDIWVNNAGVEA
                     SFPTIDMPLKEWQRVINVNLNGVFLGSREALRIFRDQKIKGSIINMSSVHQRIPWPTFA
                     HYAASKGATEMFT"
     misc_feature    complement(20835..20850)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.46"
                     /tool="antismash"
     gene            21045..22313
                     /gene="rseP"
                     /locus_tag="PO880_RS04775"
                     /old_locus_tag="PO880_04775"
     CDS             21045..22313
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene="rseP"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M50"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359768.1"
                     /locus_tag="PO880_RS04775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_04775"
                     /product="RIP metalloprotease RseP"
                     /protein_id="WP_002362168.1"
                     /sec_met_domain="Peptidase_M50 (E-value: 1.6e-83, bitscore:
                     277.2, seeds: 56, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTIITFIIVFGILVLVHEFGHFYFAKRAGILVREFAIGMGPKIF
                     AHRGKDGTTYTIRLLPIGGYVRMAGMGEDMTEITPGMPLSVELNAVGNVVKINTSKKVQ
                     LPHSIPMEVVDFDLEKELFIKGYVNGNEEEETVYKVDHDATIIESDGTEVRIAPLDVQF
                     QSAKLSQRILTNFAGPMNNFILGFILFTLAVFLQGGVTDLNTNQIGQVIPNGPAAEAGL
                     KENDKVLSINNQKIKKYEDFTTIVQKNPEKPLTFVVERNGKEEQLTVTPEKQKVEKQTI
                     GKVGVYPYMKTDLPSKLMGGIQDTLNSTTQIFKALGSLFTGFSLNKLGGPVMMFKLSEE
                     ASNAGVSTVVFLMAMLSMNLGIINLLPIPALDGGKIVLNIIEGVRGKPISPEKEGIITL
                     IGFGFVMVLMVLVTWNDIQRFFF"
     misc_feature    complement(21046..21060)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.91"
                     /tool="antismash"
     gene            22385..24103
                     /locus_tag="PO880_RS04780"
                     /old_locus_tag="PO880_04780"
     CDS             22385..24103
                     /EC_number="6.1.1.15"
                     /GO_function="GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /GO_function="GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /GO_function="GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_function="GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /GO_process="GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1162: threonyl-tRNA
                     synthetase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359769.1"
                     /locus_tag="PO880_RS04780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO880_04780"
                     /product="proline--tRNA ligase"
                     /protein_id="WP_002359769.1"
                     /transl_table=11
                     /translation="MKQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVAAGIYSYLPL
                     ANRVLEKLKTIMREEFEKIDAVEMLMPALLPAELWKESGRYETYGPNLYRLKDRNDRDY
                     ILGPTHEETFTELIRDEINSYKRLPLNLYQIQTKYRDEKRPRSGLLRGREFIMKDGYSF
                     HADEASLDQSYRDYEKAYSRIFERCGLEFRAIIGDGGAMGGKDSKEFMAISEIGEDTIC
                     YSTESDYAANLEMATSLYTPKKSHETQLDLEKIATPEVGTIAEVANFFEVEPQRIIKSV
                     LFIADEEPVMVLVRGDHDVNDVKLKNFLGADFLDEATEEDARRVLGAGFGSIGPVNVSE
                     DVKIYADLAVQDLANAIVGANEDGYHLTNVNPDRDFQPISYEDLRFVQEGDPSPDGNGV
                     LAFTKGIEIGHIFKLGTRYSDAMGATVLDENGREKSVIMGCYGIGVSRLLSAIVEQNAD
                     ERGINWPTGIAPFDLHVVQMNVKDEYQTKLSQEVEAMMTEAGYEVLVDDRNERAGVKFA
                     DADLIGCPIRITVGKKAVDGVVEVKIKRTGEMLEVRKEELESTLSILMNTTSEVE"
     misc_feature    24238..24251
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.16"
                     /tool="antismash"
ORIGIN
        1 cgtggttggc ggcggaaaca tttggcgtgg acaaattggt gctcaaatgg gcatggaacg
       61 tgcacaagct gattatatgg gcatgttagc tactgtgatg aatgccttag cattgcaaga
      121 tacattagaa aatcttggtg tgcctacacg tgttcaaaca tcaattgaaa tgcgtcaaat
      181 tgcagaacca tacattcgtc gtcgtgcaga acgtcattta gaaaaaggcc gcgttgttat
      241 ttttgccgga ggaacaggaa atccttactt ctcgacagat acaacggctg cgttacgagc
      301 tgcagaagta gatgcggacg tcatcttaat ggcgaagaac aatgttgatg gtgtttattc
      361 tgcagatcca cgtgtggatg aaacagcgac aaaatttgaa gaactaacac atttagatgt
      421 tatttctaaa gggctgcaag taatggattc aacagcaagc tcattaagta tggataacga
      481 tattccttta gttgtcttta acctaaatga agcaggaaat atccgtcgtg ccatccttgg
      541 tgaaaatatc ggaacaactg taagggggaa ataaagaatg tctaaagaag ttttagcaac
      601 agccaaagaa aaaatgacaa aagcggaaga aagcttacgc cgtgaattag gtcaaattcg
      661 tgctggtcgt gcgaacgcaa gtctattaga ccgtattcaa gtagaatatt atggtgcacc
      721 aacaccagtt aaccaattag caagtatcaa tatcccagaa gcacgtgtat taatgattac
      781 accgtttgac aaaaattcta tcgctgacat tgaaaaagct attcaaatga gtgatattgg
      841 cattagccca accaatgatg gaaatgtcat ccgtttagtg attccacaat taacggaaga
      901 acgtcggaaa gaattagcca aagacgtgaa aaaagaagcg gaaaactcaa aagttgctgt
      961 ccgtaacgtt cgtcgtgatg caatggatga gttgaaaaaa gctcaaaaaa atggcgacat
     1021 cactgaagat gaattacgtt catttgaaaa agatgttcaa aaattaacag atgatagcat
     1081 caaaaatatc gatgcgatta ctgctgaaaa agaacaagaa cttttagaag tataaaacca
     1141 atcactaata aaaattattg aatccagaga agcaaatgct ctggattttt ttgtatagat
     1201 gatgaaggcg tatctcaacg ctctctttgc gaaagtcatt ctaaagtaat tatccaagaa
     1261 aacgctttct tgaataaatt tttttattct accagaattt ttttctttgt gtagggagga
     1321 tcacttaaaa atatagagaa actttgaaat atcagccttt taaaattttc cttcacaaat
     1381 tgaatcctaa aatttgatga taagagttgt tatttttagc aattgtaatt tgcgattcat
     1441 tctcaattag gctaaaatga gtgagaacgg aaaaacgatt aaaacaagtt ggagggaatt
     1501 gtacatgtct gtgttagaaa tcaaaaattt acacgtatca attgaagata aaaagattct
     1561 aaaaggagtc gatttaacaa ttaatactgg agaaatccat gcaattatgg gacctaatgg
     1621 aactggtaaa tctactttat cagcagcaat tatgggaaat cctaactatg aagtcacaga
     1681 aggagaaatt ctatttgacg gcgtcaacgt tttagattta gaagtagacg aacgcgctcg
     1741 cttaggctta ttcttagcaa tgcaataccc aagtgaaatt ccagggatta ccaacgctga
     1801 attcatgcgt gcagcaatca atgcaaaacg tgacgaagac aacaaaatgt cagtcatgca
     1861 gttcttaaaa aaattagata gcaaaatgga attattaaat atgcctgaag aaatggctga
     1921 gcgttactta aacgaaggtt tttctggcgg cgagaaaaaa cgaaatgaaa ttttacaatt
     1981 attgatgtta gagccaacgt ttgccatttt agatgaaatt gattcaggct tagatattga
     2041 tgcgttaaaa gtagtttcta aaggggttaa tgaaatgcgc ggtgagaact ttggtgcgtt
     2101 aattattacc cattaccaac gcttattgaa ctatatcacg ccagatgttg ttcatatcat
     2161 gatggaaggc cgcgttgtga aaaccggtgg ggctgatttg gcaaaacgtt tagaaacaga
     2221 aggctatgct ggtatcagcc aagaattagg tattgattac aaagaagaag cgtaaggagg
     2281 acaagataaa tgaaaaaagt aaatctaatg gattatcttg acgaagtcac cgctttttca
     2341 ctagaacatg aagaaccagc ttggatgact gaattgcgaa caacggcttt aagaaacgct
     2401 gacgaaagtg agttaccaca tattgatcgt gtgaaatttc atcgttggcc tttattaaat
     2461 gttcacatgg aaagttatgt gccttctgaa ggaaatgtcg caagttttga tcaaatgaaa
     2521 gacaatcctt taattgttca acaaggatct tttcatgctt ttgagcaatt gccagcctca
     2581 ttagcggaac aaggggtaat ttttacagac atttttactg ctttacaaga gcacccagaa
     2641 ttagttaaag aatactatat gactaaagcc gtattaccag aagaggataa gctgacagca
     2701 gctcatgcag ctttcatgaa tagtggcgtt ttcttatacg ttcctaaaaa cgtagtgatt
     2761 gaagagccca ttgaatcttt gtttattcag gattcagata gcaaccaacc tttctttaaa
     2821 catgttttaa tcgttgcaga taatcatagt gaattcagtt atttagaacg ttttcaatca
     2881 actggtcatc acgcagagaa agcttctgga aatatcgttg tggaagtgat tgcgaaagat
     2941 ggcgcaaaag ttaaatactc agccgtggat caattagggc aaactgtaac gacttatatg
     3001 aatcgtcgtg gctatattat gcgggatgcc tctgttgatt gggctctagg cgtcatgaat
     3061 gatggcgatg ttgttgctga ttttgattct gatttagttg gcgaaggcgc tcattcagaa
     3121 gttaaagttg ttgcaattag tgcaggtaaa caaacacaag gcatcgacac acgagtgaca
     3181 aacaaagcgc cacattcaat tggtcatatt ttacaacatg gagttattcg tgaacgtggt
     3241 actttaacgt ttaacggaat tggccacatt ttaaaaggag caaaaggagc cgacgcacag
     3301 caagaaagtc gtgttttaat gctgtctgat aaagctcgtg gtgatgccaa tccaattctt
     3361 ttaattgatg aaaatgaagt gactgccgga cacgcagcca gcgttggtcg agtagatcca
     3421 gaagaaatgt attatttaat gagccgcggc ttgcgtaaag aagaagcgga acgtttggtg
     3481 attcgtggct tcctaggttc tgtcattaca gcaattccag ttaaagaagt acaaaatgaa
     3541 tttgtagaag taatcgaagg gaagttaaac gcatgatgga tgcagcaacc attcgtcaat
     3601 cgtttcctat tttatttcaa gaagttaatg atgaaccgct agtttattta gacaatgctg
     3661 cgacaaccca aaaaccaacg gcggtgttag atgttttacg acattattat gaaaccgata
     3721 atgccaatgt tcatcgtggg gtgcacacgt tagccgaacg agcaacaaag gactatgaag
     3781 cctcccgcga aaaggttcgc caatttatcc atgccaaaga aacagcggaa gttttattta
     3841 cacgaggaac aacaaccagt ttgaattgga ttgccaaaag ttatggtgac ttggcagtta
     3901 cagccgggga tgaaatcgtc atatcttaca tggaacatca ttcaaacatt attccctggc
     3961 aacaattagc caaacgtaca ggagccattt tgaagtatat agacgtcact gaagacggct
     4021 ttttagacat ggcaagtgca cgtcaacaaa ttacagaaaa aacgaaaatt gtttcgattg
     4081 ctcatgtttc aaatgtgtta ggggtcatta atccaatcga agaattaaca caattagctc
     4141 atcaaaatgg cgctgtgatg gtggtagacg gcgcgcaagc agttcctcat atgcctgtgg
     4201 acgttcaagc catcgatgct gatttttatg catttagtgg tcacaaaatg tgtggaccaa
     4261 ctggaattgg tgtcctttat ggcaaacgtc atttattgga acaaatggaa cctgtggaat
     4321 ttggtggcga aatgattgac tttgttcatc ttcaagaaag tacttggaaa gagcttccat
     4381 ggaaatttga agctggcaca cctaatattg ctggggcaat tgccttaggt gccgctattg
     4441 attatttaac tgaaattggt ttagaggcca ttcatcaaca tgaagcagcc cttgttcact
     4501 atgttttgcc gaagttgcaa gctatcgagg gcttgacgat ttatggtcca caggatccaa
     4561 aagaccacac aggtgtgatt gcttttaata ttgaaggttt acatccacat gatgtggcta
     4621 cagcgttaga tatggaaggg gtcgctgtca gagcgggtca ccattgcgcg caaccgctat
     4681 taaactattt gagcgtgcca gcaaccgcac gggtaagttt ttatttatac aataccaaag
     4741 aagacgcaga tcgcttagtt gaagcgatta aagcgacaaa ggagtttttc cagcatggca
     4801 ctttctaaat tagataactt atatcgccaa gtcattttgg accattctag ccacccacat
     4861 caccacggaa cgctagatgc gtctagccaa acaattgaac tgaataatcc tacttgtgga
     4921 gatgtgattg aactggatgt ggccattgaa gacggtgtga ttaaagatat cgcctttcaa
     4981 ggaagtggct gttcaatcag tacagcaagt gctagtatga tgactgatgc ggtcttagga
     5041 aaaacaatcg ccgaagcaac ggccttagca gaggatttct ctcaacttgt tcaaggaaat
     5101 gaagtggccg aagatgaaaa attaggcgat gcagcaatgt taagcggcgt agctaaattt
     5161 ccagctcgga tcaaatgtgc aaccttggct tggaaagcct tagagcaggc tgttgctaat
     5221 aacggccaag gcgaagccgg tcatttacat tgtgaaaaat aaagaaaggg tgacgacgaa
     5281 ttgagtacag tacctgaatt agaagaatat aaatttggct ttcacgacga tgtggaacca
     5341 gtctttagta ccggagacgg cctaacagaa gacgtcgttc gcgaaatttc tcgcgtgaaa
     5401 ggcgaaccag aatggatgct tgacttccgt ttaaaatcgt tagaacaatt caataaaatg
     5461 cccatgcaag aatggggtcc agacctgtca gatattgact ttagtaaaat taaatattac
     5521 caaaaaccga gtgacaaacc cgcacgcgat tgggatgatg tacctgataa aatcaaagaa
     5581 acctttgaaa aaattgggat tcccgaagct gagcgtgctt atctagctgg ggcttctgct
     5641 caatatgaat cagaagttgt ttaccacaac atgaaagaag aatttgaaaa attaggcatc
     5701 atttttacag atactgactc tgctttaaaa gaatatcctg acctattcaa agagtatttt
     5761 tcaaaacttg tgccgccaac agataataaa ctggccgctt taaactcagc tgtttggtca
     5821 ggtggaacct ttatttatgt accaaaaggc gttcgagttg atgtaccatt acaaacatat
     5881 ttccgaatca acgctgaaaa taccggccaa tttgaacgga ccttgattat tgtcgatgaa
     5941 ggggccagcg ttcactatgt tgaaggctgt acggcaccca cgtattcaag taacagcttg
     6001 catgcggcca tcgtagaaat tttcactaga aaagatgcct attgtcgtta taccactatt
     6061 caaaactggt cagataacgt ctacaactta gtaaccaaac gtgctaaagc ttacgaaggt
     6121 gccactgtcg aatggattga tgggaaccta ggagctaaaa caaccatgaa atacccaagc
     6181 gtttacttag atggcaaagg tgcccgtggc acgatgttat cgattgcctt tgctggcgct
     6241 aaccaaatcc aagatactgg ggccaaaatg attcacaatg cgccgaatac ctctagctca
     6301 attgtttcaa aatcaattgc taaagacggc ggcgaagtta actaccgtgg ccaagttact
     6361 tttggcaaag acagcgcagg atcgatttcc cacatcgaat gcgatacgat tatcatggac
     6421 gaaaaatcaa aatcagatac gattccgttt aacgaaatcc ataacagcca agtctcctta
     6481 gaacacgaag ccaaagtttc aaaaatttct gaagaacaat tatattatct aatgagtcgc
     6541 ggcttatctg aagccgaagc gacagaaatg attgttatgg gctttgttga accatttacg
     6601 aaagaattac cgatggaata tgcggttgag ttgaatcggt tgattagtta tgagatggaa
     6661 ggcagtgtag ggtaaaacct tttttagcgc tagaaatgtt gatttatcaa tgtttctggt
     6721 gcttcttttt ttcaaaaaag caaactaact acaatttctt ttagaaaaga tatttttcct
     6781 tttcaataaa ctgagcgaat tggtcagcgc tacgctcctt acgttcgggg tagaagtagt
     6841 tatatacttg tcgagtcatc tttgaatctt tatggccgac acggtacata atatcttctt
     6901 ttttacaaca tatttttcta gtagaaatct cttttcttta atattaagtt gtttgattgc
     6961 ttatataagc aattcatctt caaaataagt ttcaaagttt ttcaagtcaa gtgatgacat
     7021 attgataata ttaaaatagt tatatgtgta agaagaatcg agcgtgatat cgtgaaaata
     7081 ggtaaagagt agtctagtgg agtagttcct ctttttttat aatagttgtt tgcatgatag
     7141 tgtagtacac ctatatgaat gtattttttt agtttgttat cgcttggagt attgtccatg
     7201 taaactacct cctatatcta tgtgactttt cctgatgtct atactgccaa aaacttttat
     7261 gaactttttt aacaggtaag ctattatcgt ctggcatttt tggtaaaata ttatttacta
     7321 gattgttata acctaaattg aaatgtagat ttttttgtga ttacagaata aatttgtgaa
     7381 gcattcatcg gtatgccaga agttttaaaa atgtagcaga tatttttaga aataatgtcg
     7441 tgagaataga agtagtcttt attttttgat cattaaattt tgcgtaatca attttttcta
     7501 tttggtttaa tcgtttttcg attgtctcac aaagactggt aattgctttt ctctcataga
     7561 ttaattcaac tataagtttc ataagcgttt ttttcttcta ataaattcaa aataatcact
     7621 tctttattaa taagaggttt taaagccatt caataacaac aaaaaactat caattgattt
     7681 ttgatagttt ctatatagta gtatttaagg aaagtgaaga ggtagtcatc gaacatcacg
     7741 atattgaatc aatccaaatg aaattagact aagaaataag gaccagatac tttgaactaa
     7801 taaaccctga gaaccattta ataaagtagt tgtatgggtt gaaaaaaaaa gattcatggt
     7861 tgctaaatcg ggtaagtatt ttgcaaatgc agtcattgcg aataacattt gacttaatcc
     7921 aagaataaga gagaacatta ttgcaattgg taaaatatgt gatttggtaa tgatggtcaa
     7981 aaagactgta atccaagtta tttgcgtcca acttaacatt gcaataatca ctttaatcaa
     8041 aaattcaaga attaaagaga gagtaaaaga gccgttaaat tggataatac caattcctaa
     8101 gcaaattaaa ctggagaata aaccaataat ccatacgata actgttagaa gaaatagttt
     8161 agtagtaatg agttttattc gagcaggtat cgttagtaaa gttgtacgta aatatgaatt
     8221 tgaatattct tgaccgaaga aactagcagc tattataaca agtcccgctt gactcaaaaa
     8281 aaaattacgt atactcatgg ataatatctc attggttacc aaaggttgat catttccaat
     8341 tccagaaaaa aataatatta caggagctac taaaatggtt ccgactgtgc cgaataagca
     8401 ccaattataa gagaaaaatt tgattatttc actttgaaga atatttttaa ccatcaatat
     8461 tttcctccca cgtcgctacg agtaaacgaa tagaatgcta ttaacataga aaaaaatatc
     8521 catagaagaa gtatcaatgt accttttgct ggatcatgag gataagattt aaccggagta
     8581 gcaacaataa aatttcctgc tgctacgggt agatattccc cccagggcca tttctctgct
     8641 agataacttc caagattata gatttgagga actagaaata tcaacggaac aatcatattt
     8701 ctaaaaagta agccaatcaa aaaggataaa accgttaata atatccaatt taatgtagtg
     8761 tagccaataa acttccaagt agtttgactt aaaatgaatg gattcaatcc taaactacct
     8821 aatctaacat gtgtaatcgc aacggtaata aatattgaaa tgaatgaaat tgatatagta
     8881 gaaatcaaaa tagttagtag tttggcatta aaaagttctt tacgcttatt actatataac
     8941 atggttgtac gtaaattatg atttttatat tcattagctc cgctaattcc gccccatatt
     9001 acaattggaa gtactccaaa tagagcggca tcaaaaccaa aataatctaa tggaggcata
     9061 gcttctaata aatctggatg tgttgcagga gttgcatcta atcctatgga aagtaattga
     9121 tccgcttcta ataatccaag taagggttga agcacgatta ctaatccaaa aactatccac
     9181 catccacgac tatttatcag tttagaaaat tcggaataag ttactttaac aaaactcatg
     9241 attaatttcc tccctcagta agtagaaaga atacatcttc taatgtttca atattttgca
     9301 tcacttcatg aagttcacca gtaacaacaa tctgaccttt gttaatgata acaacgtcat
     9361 ccgcaatagc ttcgatttca gaaaggtagt gtgaggagag taatactgtt tttccttgat
     9421 cagcttgtct acgaataaaa tttctaaacc attttattcc catgggatct aaaccatttg
     9481 ttggttcatc aagaaccaga tattggggat ctcctagcag agcaatagca atacctaatc
     9541 tttgcccttc acctaaagat aaggttccaa gttgtgcatg acttttattt gataggtcga
     9601 caagatctaa gacttctgaa atacgtttat tcgggatgtt gttacttttt gcaatgattt
     9661 tcagatgtgt tttaactttt cttgatggag ttccgccaat cccatcaaat gccgatccga
     9721 caatttgtaa tggaaagatg aaattactat actttttttt accgaaagtg gctgttcctg
     9781 ataaaggcag gtctaaaccg agaagtattc taagtgtaga gctctttcca gctccgttag
     9841 gtccaagaaa agctgttatg cgtccttctt tagccttaaa agttatattt tctagtatat
     9901 ttttcttttt atgttgttta catatatttg aaaggacaat atttttcaat agtatggctc
     9961 ctttaaattt ttttatttaa tttgttctta aattaaaaat ttacagttct ctatccacaa
    10021 attgttcttt cgctaattct cgataaagtg gaactgtatc aattagctca ttatgatttc
    10081 ctgaaccgac aactctacca tgttcaagga cataaatgat atcagaatca attactgtgg
    10141 atagacgatg tgctacaacg ataactgttt ttttcccttt taatttatcc actgcctttt
    10201 tcattcctaa ttcatttaag ctgtccaagt tagaggttaa ttcgtcaagt aacacaattt
    10261 ctgcagatga taaaagagag cgtgccatag ccaaacgttg tctttctcca ccagagaggt
    10321 ttgcaccaga ttctccaacc tgcatatcaa gtcctttaga atttctatct aataaatgat
    10381 gaagattgac ttcttccaat acttcgatgc agtttgaatc agatatttta gaatttccta
    10441 aaagtaggtt gtcgcgtagt gtcccagata taacaggagc gtcttgttca acataagtga
    10501 tgaagttccg aagctcctct ctgctataat gcctataatc ctttccttga acgaatatct
    10561 tgccactgtc aggatcataa aatctttcaa ttaattgaag caatgtgctt tttcctgcgc
    10621 ctgagggacc tacaattgca gttctttttc ccttttctgc acgtaacgtt acttctttga
    10681 gaatcatgct atcttctttt tccttaggat cagaaccaaa tttcatttta ttatatccaa
    10741 agctgatttt ttcaaaatag actgcagttt cctcagttat taaatttgaa ctggggggaa
    10801 tagcgggagt gacatcatag tcactttcta atgcaagttg taataattcg cgaattctag
    10861 caaaagcacc gagtgattcg ccaacgcccc cgaaggcatt gaaaacttgt cctatcggac
    10921 caatcattgt atagagtagc aaagcgaatg caattaagtc agcaacagac attaaaccaa
    10981 tagacacacg gtatccacct attcccagta caattagacc agatagttgc agagtcatat
    11041 tagaaatagg agtcatgaat gcttgtattt tagctaatct tattccaata ttacgcaatc
    11101 gttctgcttg caatttagat ttttcttcct ccttgtctgt tgcgtttgtt gcacgaattg
    11161 ttctgatagc atgtaaatca cgttcgatat ctccactcaa ttttcccaac tctttttgtg
    11221 cagaaaaact agccttttgt atagctttac tcataatgat ggttccaata aaagatacac
    11281 aaactgttga aagagccata aaaaatagca aggaatctct caaaatcatc ccgatagctg
    11341 ccccaacaac aactaaaaaa ccactactga gcgcaatact cacttgtatt aaagccattc
    11401 tcaatttaga agagtcacca ctaaccctgc ttagtaaatc acctacactt cttgtatcgt
    11461 actcgtggat tggcaatctt aataaatgac taattaaact ttttcgcgct ttaaaaacga
    11521 ccccttcacc aattatttgt agcagataat attgaacagc cgttaaaatc gtagatagta
    11581 tcaatactac aatgagaatt cctaaacttg aattaatctt aattcctgta tttgattgat
    11641 caattaaatt agcaacaagg ttaggttgag cgagggttaa agctgcactg attaatccta
    11701 atattccaac gcaaatgaat tcccaattat gcccttttgc taatgcaaac agggatctaa
    11761 tatttactga gggcgtatgg tcttcaggat taacaatttc ggactccttc tttttacgtt
    11821 ctatattttc ttgttttatt tttttcaaat acgattcctt tctgagaata tcatttgaac
    11881 ggtgtggaac acatattgct ttagaatata tttgaacttc tacgggttct ccaatagggc
    11941 attcattcaa ttctatccat gcatgtgctc taaatggctc ttgtgcatac ccagtacacc
    12001 aacttactga tttacgttgt agccatagta ctattacaac agcaagcgat cgtctaaggc
    12061 atccttcttg acttctacat ctactactta cgatacacac tgcgtttcgc gcaattttta
    12121 cctgtttttc tgatgcggga ggattgtttt ttattagctt caccaatatt ttttcaattc
    12181 tcccagggga catccaatta agcggtgcag atagtattaa actcaaacga gccagaaatc
    12241 ttgcactaaa agataccgga tctatttgtt ctggaaaaag ttggatattc attatgcaag
    12301 tttcatctcc ttcaaaagag ccataaactc ggcaacatct gtttccgctt gagctttatc
    12361 tagagaaaat gaagtttcca attgtagtgc taactcttct atcgtcaatc ctttactcaa
    12421 accttctaac atagaaactc cagaagcatt tacttggaaa taaattcctc tacttttatc
    12481 caaaacaatt gcaccatcat cttggctaat aattttcaac tctcttttta cttttctcat
    12541 taatattact cctccttttt agaaaataac tacaactagg taagcagcaa cttcctttta
    12601 ttttgagttc ttttttaaag ctacacgaag ccagcgttca acagctgcta atctctcaaa
    12661 ggcttcaact ctagtatgta gagcagaagg cattgataat tcagaaatta gtatatctgc
    12721 atctacaatt cctaattcaa ctaatttgaa ttggtgcact tgctgggaat gcttttttac
    12781 ggcttctttg taacttttat acaaacttgt ggaatagtct cctttaaatc cacgtgtaaa
    12841 aatatttttt gaaacaagtc ctttagtggc attatataaa atcgattttg ttacagtgtg
    12901 atcttgtctg taacgagctg gtatagctaa cgagtaatta ataatatcga catctaaaaa
    12961 gggagcttgc caagtcatac cattaacacc atatatgttg ttaatctgag ttaccaaatg
    13021 tctttgaaaa agtagagact cgatcatttg gtgtcgagaa cgatcggaat ctaaagaatt
    13081 cgatgtgtga tctaatttat ttattagaac tttataactt aattctcggt aaggcttcgt
    13141 aagccaatta ggtaccgtga ctggaccgca ccagttgagt gcggagtttt gacgagttgg
    13201 tttagcataa ccaaatcctt tgtctatttc attttttaca gcttccttaa aggaattatt
    13261 atttattaga tctactgtcc caactgaaat aggaatccga tttagtaaac aatatcgtaa
    13321 gcctaagtct atactgcgta atttattttg tcgtacaaga gaccatgcgt tagcaggcat
    13381 aggagtgaat aattcatccc cgccaagacc tgtaaaatga gtggcattgg cgggagttaa
    13441 tgctgaatgt tctatggatt caacatatcc ctctgtatct gcccaaaata atggagaatc
    13501 tggtaacact gagtttgagt aatctctatc aatttcaaaa ttttttcccg agctacctaa
    13561 agaaggtaat gaagtaaata tgacgttgat atcgtttgag attaattttg cccatgtaga
    13621 atcgctattc cattcactat cagcatttgc gtgataaagt ttaacattag cttgaagcgc
    13681 ttttaagata taaataattg ttgctgaatc aactccaccc gaaaggtcag cggatatctc
    13741 agagtaacta actgtatttt gactaagcaa ttctagaagc cgctctcgta tttttgaata
    13801 aatagattcg actttgtttt ctaagtttgg aggtgtccaa gtttgtatgg gttctaagat
    13861 tccatttgtt gtacatttca aaatatagaa tggttggatc gtgtttactt tttcccataa
    13921 gggaatattt tggaatgggt agtatggtaa attatgaatt agatttaatg caagagccga
    13981 tttagaaatt ggtaaattaa gaaataatgc gatatttgct gatgaatcac taatgattag
    14041 ttcatcattt gagtccaacc atgaaagaga aacatcaaat aatgatcctc ccataaatag
    14101 cccttcttta tttattaaaa caaatacgcc tttagaaata tttgcttgaa gataatctaa
    14161 catgttttct gtagtagtgt agttaaattg gtctttattg caaaatattc ttgaatattc
    14221 agaagaaata cagtaaattt caaattgatc gtgtgtatgt tgagagtact cggtattgga
    14281 taccagcagt atattagtgt agtctgtagt aatacgtttt tgaaacatca atttttttga
    14341 ggatatagag atatttgatt tgtttacaat aattagaagc acctccctaa ataaagacta
    14401 tgcggattac taaaaaggtc atcgaattat gtaattcgat gaccaatggc ataattatgt
    14461 aattcgatga ccgctagcat aattatttcc catttttctg tcctagtaat aaattacaac
    14521 ttggaaaaga gcgcgtccac caaatacatc tctaaattta cctgtccaaa gaccacgagt
    14581 agattcttta aaacctgcaa ttttttcaat tgatggttta ctgtaatttt gctttgtcat
    14641 agagttcacc tccttcatag ttggtgtata ctaaattttc taattcaagt aaattagaaa
    14701 atagttacct tgattactta gtactgaaaa atgagtatag gaaattcttg acatctatat
    14761 tttagttaaa caatagaaca aaacagctag aatcaggaaa taaattactc ttaattcgtt
    14821 gtttttaagg ttaagtagct tttttctatc aacttatata ttaatcatac aaaaaaaggg
    14881 attatttgta atgagtatat tgtgcaatta tttaagaaag agtaagatga agatatcatt
    14941 aaaaaataca cagagtggag tatgaataaa atctgtgttg tttaatacat tagtgggata
    15001 ttagaaaatt ttattcaaac tataaattca aaattagtat ctttaaaaaa acgaagaact
    15061 gtagaatatt caaggaatac ttagattgga gaaaagtgta tgaaagaaaa atttgtttat
    15121 ttgttatcct tatttgtatt cttagtgata gctctactta ctattaagca gaatgactat
    15181 ttgtatggat cgcaagcagt aactgttgta ccgattattg ttgtacttat tcttttttgc
    15241 gcagttatta tttttgtctc taaagatatt aaaaagatta gatacttcgc tgtacttact
    15301 ttttttactt ccatttcctt agcaatagga ttatttcctt attcttttgt gcatcctgtc
    15361 aaatccctgc taatttcatt tttaccttac tttgtttata agttttttgt cacttttaac
    15421 ttaatttcga ttttatctgt ttctaaaaaa attgatcaaa ttttatttat acaatctatt
    15481 attgtatcca tgttactgct tcttaatttt gaaaaattca tatggatggt gagtatagta
    15541 atcattttgg atatttttgg ttgtttattt ttatatttta aaggaaaaga tcgactaacc
    15601 attcgaacaa aaaaagaaca agtttttcta attcgaatac tagttttatc gctattttcg
    15661 ttaattattt ctacttctat tttaagtggg ttggttcttt ttcttatccc aatcacagtc
    15721 ggaattattt taataaaacg taatgaaatt agcttagata aagtatcaat tattataaat
    15781 ttagttatcc atattatttg tataggaatc ttcttcatta ttaattttta ttttcttcga
    15841 ttatcaatac ttgatatcta ttttatttct attttttatt gtctgatttt gtttatcctt
    15901 tatttgaaga attggtattt gagtagtatt gaggtgagcc gaattcaaca aagaaagaaa
    15961 gatttccaaa aagaaaagat cgattttgta caaactatca gtaaagaaaa gactttgtct
    16021 tcagtaagta ctttaattag tacaatcttt ttgcaacatt acgaaataaa tagttattta
    16081 atggtttgga aagatcaata ccaaccatat attttgtcta gctcaggaga attttctaaa
    16141 ctgattttaa cgaataaaat tattgctgaa tttgaacgcg actctaacat attccattat
    16201 cacaacaata aatatagaaa attcactttt tttgagaata aacaaatttt aggttggtta
    16261 ctcataggta gagaaaaaga ctttgacttg gcgagtatga aacatgaaga aatatatgat
    16321 ttagttggtg ttgctggtaa aataattatt gaaaatgaac gcttatatga tattaaacaa
    16381 gaatcattaa aaatagaaag tttatcttat gatgaatacg tgaattatga atatttaaac
    16441 atggtgcaaa attttcataa agatttttca ttttttattc atgacaatgt tttacaaaat
    16501 attttggctt taaaaaaact aatcgaatct atgtctactg aaaatgtaga aacaaagaaa
    16561 ttagtattag aaacttttga tactttaaat aaaactttta gagacaagat atttgaatta
    16621 tatccttcga cgattgaaac agtgcctgtg tcgcaaagca ttcgaattct ttgtgataaa
    16681 ttcaatgagg gacaagaatc tatatttgtt catttttatt gtccacaaga aatgcatcta
    16741 aaaaaagaag agaaatttca tatttatcga attatacaag agttaataac taatgctcta
    16801 aaacactctc gagcaacagt agtttgtgtt tatctcattc aaagggactt ttatatagaa
    16861 tgtattgtac aagatgatgg cacaggcttt gattatagca aaataaaact tagaggattt
    16921 gaaaataaac attttggtat tctatcaata aatcaagagg ttaattcatt aaatggaaaa
    16981 atgagagtat catctgtaaa tccacatggt acaaaattta caataacttt accgattgca
    17041 aaggaggata tagattcatg aaagtatatt tattagacga tcatgcactt ttctctaaaa
    17101 gtcttgaaat tgcatttcaa ccaatggatg tctcaattac tagttttact aatccagaat
    17161 tttttttaaa taaactaaaa aatgagcaac cagatttagc attattggat atccatattg
    17221 gagagttaag tggatttgaa atatctaaag aaattattgg aaaatttcca aatcaaaaaa
    17281 tagttttttt atcaggtttt gatttaattg aatacaagaa tgaagcaata aaatctggag
    17341 catggggact attaaataaa aatgtaacaa ttgaagaatt gtatgatgat ttacaaaaaa
    17401 tacatcatgg aactgatctg ctacccaagt gccaaagtac attagatgtt cttagtaaac
    17461 gagaaaaaga gatactaaaa tgtgctgcag aaggttttaa gcagcaggag atagcagaac
    17521 aacttcatat cagtagaaga acagtaaata atcatcttgt atccataaat aataagctag
    17581 aggtaaattc aaccatttct gcaattattc aagctattga attgggaatt attagagtaa
    17641 aaggatattg atactaaaaa atgttgaaat tatcaaacat ttattcagaa agatatgctg
    17701 tttacggaat accataaccc atgatataag ggctattttt tcataactta gtttagcaat
    17761 tttgtttcct gaattctctt caattgtgta aacaacttca ttttctactt tctcaacaat
    17821 cccaacgtaa taactgatag aatcaccatc ccaatcaaaa aagatgatat tcccattttt
    17881 aggcaaactt cctttcctaa accattggtt attcgcttta aaattttcaa taccagttgg
    17941 gcaataggca aaatgttcca ttttaactcc agctttttca gcattataag aaacaaaaat
    18001 agcgcaccat tctacacgtc cattaaagcc ataccattgc caatatttcg ttccaccctt
    18061 atgtattcct tcattcaatt ctttaagtgc tgcttttaca atagctgaat tcccttgact
    18121 attgttgttg gaaatagaag tgatgtattg tttaacaatt tgagcataaa acatattgcc
    18181 atacgcataa cgataatcat agcctatgtt gatagctata ggattagtgt aagtgactgt
    18241 ttttccatct gcttgagttt ttgagaattg aatcgtaata aagtggatga ttgatcacct
    18301 ccataatccg attaaaaaac tcagatttag ggtcaacgta caagcctttt ccattcatta
    18361 aaacggagag gaagaaaatg aactttgcca agtaagattt tccgtttaca gtgggtccag
    18421 tgatggcaat gtgcgggcta ttggcttttt tatcggtgat atcatttagt ataattgtac
    18481 tataaaacaa ttgaacacat tgaaagggga agaaatgtac aaattcaaaa agtacaaata
    18541 taaaggcgag gagcattatt ttactggtgt tactcttttc aaagtgaacg acaataaaat
    18601 tatttcgctt aaagaattta aatcaataac aaatacttat cgtcctttca agtagaataa
    18661 tttagtgttg cacttaaact gtaacactag gtttattata aaaacgtacg ttcttgtttg
    18721 taaataactc ttttgtcgta attaatttat tcatgaaaac tgactttgtt atagttattc
    18781 gtttagctcg catttttctt actaaaaact atccaaaagt atccatattt ttatagttga
    18841 aacggtttga aactaaatca aaactaacta caattgaaaa aaatcactgg tatttcacaa
    18901 cattctaaga aaaagaataa tgctgtcata ccaacgatta gagcttatca gaagctctta
    18961 aaaaaactaa ccctttgctg gaaggctctg tcggctagtt tttcactcta ccaacaaaat
    19021 aatcaaccaa aataacaacg gaaagccaaa caagaaacac actaatcatg cttcttcaaa
    19081 gaaagtacaa ataaaaaagc gatgactcga gaagaaaatc tctatggagt catcgctttt
    19141 tcttatattt tagttaaaaa attattttac taaaggttga agtaacttgt tccagaaact
    19201 aatgagtggg tcagcaccaa atgcacaaat aattgtacca atcccaattg gaccaccagc
    19261 aataaagcca attaatagga ccaacaaatc ttgtgctttt cgaacagaag cataaggttt
    19321 gttgatttgt tgacagatgg ttggcgcaat cgcatcatag ggggactcgc cttgatctgt
    19381 agccatgtat aaggcgcagc caaatgtaaa aatcaggact ccgacgaata aagaaagtag
    19441 tttgccaatt gttgagcctc ctaaactatt ggcgtaaggt tgaagccaag tagaaatctg
    19501 ttgaatcaaa attcctacta aaagcatatt taaaatagtt cctagaccaa tggctttttt
    19561 gttttttaaa tagacataga ataaaataaa cgcattgaca ataatttgaa aaatgcctaa
    19621 ttgcatacca aacatcgaag aaagtccgat attcatagca gtgaaaggat caactcctaa
    19681 acctgccact cttaaagtgg atgcgccaat cccgataata actaaaccaa ttaaaattaa
    19741 aagaacattt ttgactagtt tcatggtaag cctcctgaat agattttgtg tgtgatagta
    19801 caaagtctgc aacaaaaatt ctctatgaat ttttgttgca gactactttt taaccagctc
    19861 cgtgttggaa cgaagggtat agagacatcc cgccatctac aaagagtgtt gttccggtta
    19921 cgtatgagga ttcatcagaa gctaaccaag cggccgcagc agctacttga tttggtttac
    19981 caataatgcc cataggaacc atgctcgttg tttgttttaa ctgttcggga tcggaaaatt
    20041 tttcagcatt aataggtgtg ttaatcgctc caggtgcaat acagttggca cgaataccat
    20101 attccgcata ttccaaagcg attgttttcg tgaacatttc ggttgcgcct ttacttgccg
    20161 catagtgagc gaacgttggc caaggaattc tttggtgaac ggaggacata ttaatgattg
    20221 accctttgat tttttgatct ctaaatattc ggagtgcctc tctagaaccc aaaaagacac
    20281 cgtttaaatt gacattaatg acacgttgcc attcttttaa tggcatatca attgtcggaa
    20341 aagaagcctc cacaccggcg ttatttaccc aaatgtcaac atgaccaaac tcttttaaag
    20401 cggtttcagc taacgcttta atatcctctt cattagagac atcagccttc acgaatttta
    20461 cgcggtctgc gtgcttggct tggacatctt caaattcttt aggcagctca ccgacatagt
    20521 cagcaacaac attcatgccc tctaagatat aacgcagagt aataaataat cctaaacccg
    20581 tagctgcacc agtgacaacg gccactttac catgtaattc tccatacatt gattcattcc
    20641 ccctaaaatt actattaata ctttcatggt agcacatgtc aatcagcgtg ttgataaaat
    20701 acgacaaact gtcggaaaag tttatggtct aaagttagct actattgttg aaggtcttta
    20761 aaaaaatact ggtcgtagta atcaattcct ctcatttata gagttttcta aaaaaacaca
    20821 aaatatctag gcgaagtggt caagtccaat ggttatttat gagtgaatat gctaaaataa
    20881 aacttgtctt acataaggaa agatttgttc actaacagtg gcgactgttc tcagtaaaca
    20941 gttgcactgg tttatcggga agggaacaga gcaagtttta cgagactttc ccatgtaata
    21001 ataaaaagca ctgaacgtca gtgaatgaag gaagaaggac atctatgaaa acaattatca
    21061 cattcattat tgtcttcggt attcttgtcc tcgtacatga atttggccac ttttattttg
    21121 caaaacgagc gggtatttta gttcgtgagt ttgcaatcgg aatgggacca aagatttttg
    21181 cgcatcgtgg aaaagatggc accacttata cgattcgctt attgccaatt ggtggctatg
    21241 tgcgaatggc tgggatgggc gaagacatga cagaaatcac accaggtatg cctctatctg
    21301 ttgagttaaa tgccgtgggt aatgtggtta aaattaatac aagtaaaaaa gtacaattac
    21361 ctcatagtat tccgatggaa gtcgttgatt ttgatcttga aaaagaatta ttcatcaagg
    21421 gctatgtcaa tggaaacgag gaagaagaaa ccgtttataa agttgaccat gatgcaacga
    21481 ttattgaaag tgatggaacc gaggtgcgga ttgcgccact tgacgttcaa tttcaatcag
    21541 cgaaattatc gcaacgcatt ttgacgaact ttgcgggacc catgaataac tttatcttag
    21601 ggtttattct gtttacgtta gcggtctttc tacaaggagg cgttactgat ttaaacacga
    21661 atcaaattgg acaagtgatt cctaatggcc cagccgcaga agctgggttg aaagaaaatg
    21721 ataaagtctt atcgattaat aatcaaaaaa tcaaaaaata cgaagatttt acaaccattg
    21781 tgcagaagaa ccccgaaaag ccgttaacgt tcgtagttga gcgtaacggc aaagaagagc
    21841 aactaacagt gacaccagaa aaacaaaaag tggaaaaaca aacgattggt aaagtcggcg
    21901 tttatcctta tatgaaaacc gatttaccgt caaaattgat gggcggtatt caggatactt
    21961 taaatagtac gacacagatt tttaaagcac tcggctcact attcacaggc tttagtttaa
    22021 acaaactagg tgggccagtc atgatgttta agttatcgga agaagcatcc aatgctggag
    22081 taagtacagt tgtattctta atggccatgt tgtcaatgaa cttagggatt attaatttgt
    22141 tgccgatccc agctttagat ggcgggaaaa ttgtcttaaa cattattgaa ggtgtacgtg
    22201 gaaaaccaat tagtcctgaa aaagaaggca tcattacgtt aattggcttt gggtttgtca
    22261 tggtgttaat ggtgttagtt acttggaacg atattcaacg ctttttcttt taaggagaaa
    22321 gcttaagtaa aaaagtgtgt aaagaaatca aaaatagttg attttaaaat agaggagagt
    22381 tttaatgaaa cagtcaaaaa tgttaattcc aacgttaaga gaagtaccaa atgatgcaga
    22441 agtcttaagt catcaaattc tgttgcgcgc aggctatatt cgtcaagtcg ctgcagggat
    22501 ctattcttat ttacctttag ccaatcgtgt tttagaaaaa ttaaaaacga ttatgcgaga
    22561 agaatttgag aaaattgatg cagttgaaat gttaatgcct gcgttattac cagcagaact
    22621 ttggaaagaa tcaggccgtt atgaaactta tggacctaac ttgtatcgct taaaagatcg
    22681 caatgaccgt gactatatct taggcccaac gcatgaagaa accttcacag aattaattcg
    22741 tgatgaaatc aattcttaca aacgattacc tttgaattta taccaaattc aaacaaaata
    22801 tcgtgatgaa aaacgccctc gttctggctt attaagaggc cgtgaattta tcatgaaaga
    22861 tggctattct ttccatgccg atgaagctag tttagatcaa tcataccgtg attacgaaaa
    22921 agcttactca cgcatttttg aacgctgtgg actagaattc cgtgccatta ttggggacgg
    22981 tggtgccatg ggtggtaaag attctaaaga atttatggcg atttctgaaa ttggggaaga
    23041 tactatttgt tattcaacag aaagtgatta tgctgccaat ttagaaatgg caacaagtct
    23101 ttatacgccg aaaaaatcac atgaaacaca attagattta gaaaaaattg caacaccaga
    23161 agtgggtaca attgccgaag tggcaaactt ctttgaagtt gagccacaac gaattattaa
    23221 atcagtacta tttattgccg atgaagagcc agtaatggtc ttggttcgtg gcgatcatga
    23281 tgtcaatgat gtcaaattga aaaacttctt aggcgctgat ttcctagatg aagcaacaga
    23341 agaagatgct cgccgtgtct taggagctgg ctttggttca attggtccag tcaatgtttc
    23401 cgaagatgtt aaaatttatg cagatttagc tgttcaagat ttagccaacg ccattgttgg
    23461 ggctaacgaa gatggctatc atttgacaaa tgtaaatcca gatcgtgatt tccaaccaat
    23521 tagttacgaa gatttacgtt ttgttcaaga aggcgatcct tctcctgatg gcaatggtgt
    23581 cttagccttt actaaaggaa tcgaaattgg tcatatcttt aaattaggaa cgcgttacag
    23641 tgatgctatg ggcgcaacag ttttagatga aaacggtcgt gaaaaatcag tgatcatggg
    23701 ttgctatggc atcggcgtta gtcgtttact ttcagcaatc gttgaacaaa atgcagacga
    23761 gcgcggtatt aattggccaa caggaattgc gccatttgat ttacacgtgg tccaaatgaa
    23821 tgtgaaagat gagtaccaaa caaaactatc acaagaagtt gaagcaatga tgaccgaagc
    23881 gggctacgaa gtattagttg atgaccgcaa tgagcgcgca ggcgtaaaat ttgctgatgc
    23941 tgatttaatt ggttgtccaa tccgtatcac agtcggtaaa aaagcagtcg atggtgtggt
    24001 agaagtcaaa atcaaacgta ctggtgaaat gttggaagta cgcaaagaag aattagaaag
    24061 tacactttct attttgatga acacaacatc agaagtggaa taataatgaa aagggcggac
    24121 agaatgatta ggataaactg tttccgccac ttatcagcgt ttaagcttga agcaaaaatc
    24181 taaagtgatt tttgcttcaa gctcttctta gctcttttta gaaaagaaac aagattgttt
    24241 ttttacaaaa aacgagtata cttaatagag aagcgaatgc agagaaaccg aaaaaaggca
    24301 cttgacagat ttgtctgagg gccttttttc aatgatagaa ggaggttcat tttgtgacag
    24361 aaaaagcacg cgatttattt ga
//
