LOCUS       NZ_JAQOXM010000013      1424 bp    DNA     linear   CON 10-MAR-2024
DEFINITION  Bacillus subtilis strain CWTS 5 NODE_13_length_1424_cov_28.5143,
            whole genome shotgun sequence.
ACCESSION   NZ_JAQOXM010000013
VERSION     NZ_JAQOXM010000013.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Natarajan,D.A., Chandwani,S. and Dewala,S.
  TITLE     Complete genome sequencing of Bacillus subtilis (CWTS 5), a
            siderophore-producing bacterium triggers antagonistic potential
            against Ralstonia solanacearum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Natarajan,D.A., Chandwani,S. and Dewala,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2023) Molecular Biology Unit, Uka Tarsadia
            University, Bardoli, India, Maliba campus, Bardoli, Gujarat 394620,
            India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-JUL-2022
            Assembly Method                   :: SPAdes v. 3.11.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 02:26:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,268
            CDSs (total)                      :: 4,150
            Genes (coding)                    :: 4,020
            CDSs (with protein)               :: 4,020
            Genes (RNA)                       :: 118
            rRNAs                             :: 10, 12, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 11, 6 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 77 of 130
            Pseudo Genes (internal stop)      :: 19 of 130
            Pseudo Genes (multiple problems)  :: 23 of 130
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:37:08
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 1424
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAQOXM010000013.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     CDS             complement(<1..1422)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (253-322). E-value: 3.3e-26. Score:
                     83.3. Matches aSDomain: nrpspksdomains_PO770_RS20945_PCP.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010330629.1"
                     /locus_tag="PO770_RS20945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_20950"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_272512150.1"
                     /sec_met_domain="AMP-binding (E-value: 1.7e-63, bitscore:
                     200.7, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2.5e-18, bitscore:
                     52.7, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 2.8e-48, bitscore:
                     150.6, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGTNLKRVFAGGEPLAPRTAARFASVLPQVSLIHGYGPTEATVDA
                     AFYVLDPERDRDRLRIPIGKPVPGARLYVLDPHLAVQPSGVAGELYIAGAGVARGYLNR
                     PALTEERFLEDPFYPGERMYKTGDVARWLPDGNIEFLGRLDDQVKIRGYRIEPGEIEAA
                     LRSIEGVREAAVTVRTDSGEPELCAYVEGLQRNEVRAQLESLLPGYMVPAYMIEMEQWP
                     VTPSGKLDRNALPAPGGAADAETYTAPRNVTEMKLSQLWEDVLKNGPVGIHDNFFDRGG
                     HSLKATALVSRIAKEFDVQVPLKDVFAHPTVEGLATVIRKGTDSPYEAIKPAEKQETYP
                     VSSAQKRIYVLQQLEDGGTGYNMPAVLELEGKLNPERMDRAFKELIKRHESLRTSFEQD
                     AGGDPVQRIHDEVPFTLQTTVLGERTEEEAAAAFIKPFDLSQAPLFRAQIVKVSDERHL
                     LLVDMHHIISDGVSVN"
     source          1..1424
                     /altitude="22 m"
                     /collected_by="Dr. Natarajan Amaresan"
                     /db_xref="taxon:1423"
                     /identified_by="Dr. Natarajan Amaresan"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Bacillus subtilis"
                     /strain="CWTS 5"
                     /submitter_seqid="NODE_13_length_1424_cov_28.5143"
     gene            complement(<1..>1424)
                     /locus_tag="PO770_RS20945"
                     /old_locus_tag="PO770_20950"
     region          1..1424
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..1424
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..1424
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:1422](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..1422)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS_motif       complement(234..341)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20945_0003"
                     /evalue="2.00E-17"
                     /label="C2_LCL_024-062"
                     /locus_tag="PO770_RS20945"
                     /protein_end="396"
                     /protein_start="360"
                     /score="55.6"
                     /tool="antismash"
                     /translation="YNMPAVLELEGKLNPERMDRAFKELIKRHESLRTSF"
     aSDomain        complement(456..662)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20945_PCP.1"
                     /evalue="3.30E-26"
                     /label="PO770_RS20945_PCP.1"
                     /locus_tag="PO770_RS20945"
                     /protein_end="322"
                     /protein_start="253"
                     /score="83.3"
                     /tool="antismash"
                     /translation="EMKLSQLWEDVLKNGPVGIHDNFFDRGGHSLKATALVSRIAKEFD
                     VQVPLKDVFAHPTVEGLATVIRKG"
     CDS_motif       complement(939..1004)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20945_0002"
                     /evalue="2.10E-13"
                     /label="NRPS-A_a8"
                     /locus_tag="PO770_RS20945"
                     /protein_end="161"
                     /protein_start="139"
                     /score="41.9"
                     /tool="antismash"
                     /translation="FLGRLDDQVKIRGYRIEPGEIE"
     CDS_motif       complement(1086..1175)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20945_0001"
                     /evalue="2.50E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="PO770_RS20945"
                     /protein_end="112"
                     /protein_start="82"
                     /score="58.1"
                     /tool="antismash"
                     /translation="PSGVAGELYIAGAGVARGYLNRPALTEERF"
ORIGIN
        1 tgttgacgga aacaccgtct gaaatgatgt gatgcatatc caccagcaac aggtgccgct
       61 catctgatac ctttacgatt tgggcacgga acagcggcgc ttggctgaga tcaaacggtt
      121 tgataaatgc ggctgcggcc tcctcttcgg ttcgttcgcc gagaactgta gtctgtaacg
      181 taaacggcac ctcgtcatgg atgcgctgca ccggatcgcc tcctgcgtcc tgttcgaagg
      241 aggtccgaag cgactcgtgg cgcttgatca gctctttgaa agctctgtcc atcctttcag
      301 ggttgagctt cccttcgagc tctaacacag ctggcatatt gtagcctgtc cctccgtcct
      361 ccagctgctg aaggacataa atccgctttt gggcagagga aaccggatac gtttcctgtt
      421 tttcagcggg cttaatcgct tcatatggac tgtccgttcc tttacggata acggtagcca
      481 gtccttctac cgtcggatgg gcaaacacat cttttagcgg cacctgtaca tcgaattctt
      541 tggcaatccg tgagacaagc gccgttgctt ttaaggaatg tcccccgcgg tcaaaaaagt
      601 tgtcgtgaat cccgacaggg ccatttttca gcacgtcctc ccacagctga gaaagcttca
      661 tctcagtcac gtttctcggc gctgtgtagg tctccgcatc tgccgctccg ccaggagctg
      721 gcagggcgtt tcggtcgagc tttccgcttg gtgtaaccgg ccactgttcc atttctatca
      781 tataggctgg gaccatgtag cccggcaaca ggctctcaag ctgcgctcgg acttcgtttc
      841 tttggagccc ttctacataa gcgcataatt ccggctcgcc gctgtctgtc cgtacggtaa
      901 cggccgcctc tcttacacct tctatacttc ttagtgccgc ttcaatttct cccggttcaa
      961 tccggtagcc gcgaattttc acctgatcat ccaaccgtcc caggaattca atattcccgt
     1021 cagggagcca gcgcgccaca tctcctgttt tgtacatacg ttcacccggg taaaacgggt
     1081 cttctagaaa acgctcctcc gttaatgccg gccgattcaa ataacctctg gcaacacccg
     1141 ctccggcaat gtatagttcg ccggcgacac cggaaggctg tacggctaaa tgcggatcta
     1201 aaacatacaa acgcgcacca ggtacgggct tcccaatcgg aatccgcaaa cggtctctgt
     1261 cccgttctgg atccaatacg taaaatgccg cgtctaccgt tgcttctgtc ggcccgtagc
     1321 catggattaa tgatacctgc ggcaatacag aagcaaaacg ggctgccgta cgcggcgcaa
     1381 gcggttcacc tccggcgaat acacgcttta ggtttgtccc gtca
//
