LOCUS       NZ_JAQOXM010000011      9942 bp    DNA     linear   CON 10-MAR-2024
DEFINITION  Bacillus subtilis strain CWTS 5 NODE_11_length_9942_cov_33.4822,
            whole genome shotgun sequence.
ACCESSION   NZ_JAQOXM010000011
VERSION     NZ_JAQOXM010000011.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 9942)
  AUTHORS   Natarajan,D.A., Chandwani,S. and Dewala,S.
  TITLE     Complete genome sequencing of Bacillus subtilis (CWTS 5), a
            siderophore-producing bacterium triggers antagonistic potential
            against Ralstonia solanacearum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9942)
  AUTHORS   Natarajan,D.A., Chandwani,S. and Dewala,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2023) Molecular Biology Unit, Uka Tarsadia
            University, Bardoli, India, Maliba campus, Bardoli, Gujarat 394620,
            India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-JUL-2022
            Assembly Method                   :: SPAdes v. 3.11.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 02:26:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,268
            CDSs (total)                      :: 4,150
            Genes (coding)                    :: 4,020
            CDSs (with protein)               :: 4,020
            Genes (RNA)                       :: 118
            rRNAs                             :: 10, 12, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 11, 6 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 77 of 130
            Pseudo Genes (internal stop)      :: 19 of 130
            Pseudo Genes (multiple problems)  :: 23 of 130
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:37:08
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9942
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAQOXM010000011.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..9942
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:>9938](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..>9938)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            complement(<1..3331)
                     /gene="srfAB"
                     /locus_tag="PO770_RS20910"
                     /old_locus_tag="PO770_20915"
                     /pseudo=""
     CDS             complement(<1..3331)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: AMP-binding (12-415). E-value: 3.1e-116.
                     Score: 380.3. Matches aSDomain:
                     nrpspksdomains_PO770_RS20910_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (521-589). E-value: 1.8e-25. Score:
                     80.9. Matches aSDomain: nrpspksdomains_PO770_RS20910_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (605-892). E-value:
                     3e-106. Score: 346.7. Matches aSDomain:
                     nrpspksdomains_PO770_RS20910_Condensation_LCL.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="PO770_RS20910"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_20915"
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="AMP-binding (E-value: 2.9e-126, bitscore:
                     409.7, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 9.2e-18, bitscore:
                     53.1, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1e-99, bitscore:
                     321.8, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQITEATFAALFEKQAQQTPDHPAVKAGGNLLTYRELDEQANQL
                     AHHLRAQGAGNEDIVAIVMDRSAEVMVSILGVMKAGAAFLPIDPDTPEERIRYSLEDSG
                     AKIAVVNERNMTAIGQYEGTIVSLDDGKWRNESKERPSSISGSRNLAYVIYTSGTTGKP
                     KGVQIEHRNLTNYVSWFSEEASLTENDKTVLLSSYAFDLGYTSMFPVLLGGGELHIVQK
                     ETYTAPDEIAHYIKEHGITYIKLTPSLFHTIVNTASFAKDANFESLRLIVLGGEKIIPT
                     DVLAFRKMYGHTEFINHYGPTEATIGAIAGRVDLSEPDAFAKRPTIGRPIANAGALVLN
                     EALKLVPPGASGQLYITGQGLARGYLNRPQLTAERFVENPYLPGSLMYKTGDVVRRLSD
                     GTLAFIGRADDQVKIRGYRIEPKEIETVMLSLSGIQEAVVLAVSEGGLQELCAYYTSDQ
                     AIEKAELRKQLSLTLPSHMIPAFFVQVDAIPLTANGKTDRNALPKPNAAQSGGKALAAP
                     ETALEESLCRIWQKTLGIEAIGIDDNFFDLGGHSLKGMMLIANIQAALEKSVPLKALFE
                     QPTVRQLAAYMEASAVSGGHQVLKPADKQAMYPLSSAQKRMYVLNQLDRQTISYNMPSV
                     LLMEGELDISRLRYSLNQLVNRHESLRTSFMEANGEPVQRIIEKAEVDLHVFEAKEDEA
                     DQKIKEFIRPFDLNDAPLIRAALLRIEAKKHLLLLDMHHIIADGVSRGIFVKELALLYK
                     GEQLPEPALHYKDFAVWQNEAEQKERMKEHEAYWMSVLSGELPELDLPLDYARPPVQSF
                     KGDTIRFRTGSETAKAVEKLLAETGTTLHMVLHAVFHVFLSKISGQRDIVIGSVTAGRT
                     NADVQDMPGMFVNTLALRMEAKEQQTFAELLEQAKQTNLSALEHQEYPFEDLVNQLDLP
                     RDMSRNPLFNVMVTTENPDKEQLTLQNLSISPYEAHQGTSKFDLTLGGFTDENGIGLQL
                     EYATDLFAKETVEKWSEYVLRLLKAVADNPNQPLSSLSLVTETEKQALLEAWKGKALPV
                     PTDKTVHQLFEETVQCHKDCPAVTYNGQSWTYGELNAKANRLARILMDCGISPDDRVGI
                     LTK"
     source          1..9942
                     /altitude="22 m"
                     /collected_by="Dr. Natarajan Amaresan"
                     /db_xref="taxon:1423"
                     /identified_by="Dr. Natarajan Amaresan"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Bacillus subtilis"
                     /strain="CWTS 5"
                     /submitter_seqid="NODE_11_length_9942_cov_33.4822"
     region          1..9942
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..9942
                     /SMILES="NC(C(C)C)C(=O)NC(CC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     CDS_motif       complement(503..616)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20910_0009"
                     /evalue="6.40E-18"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="PO770_RS20910"
                     /protein_end="943"
                     /protein_start="905"
                     /score="57.1"
                     /tool="antismash"
                     /translation="KQTNLSALEHQEYPFEDLVNQLDLPRDMSRNPLFNVMV"
     aSDomain        complement(656..1516)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20910_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="3.00E-106"
                     /label="PO770_RS20910_Condensation_LCL.1"
                     /locus_tag="PO770_RS20910"
                     /protein_end="892"
                     /protein_start="605"
                     /score="346.7"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIADG"
                     /tool="antismash"
                     /translation="AMYPLSSAQKRMYVLNQLDRQTISYNMPSVLLMEGELDISRLRYS
                     LNQLVNRHESLRTSFMEANGEPVQRIIEKAEVDLHVFEAKEDEADQKIKEFIRPFDLND
                     APLIRAALLRIEAKKHLLLLDMHHIIADGVSRGIFVKELALLYKGEQLPEPALHYKDFA
                     VWQNEAEQKERMKEHEAYWMSVLSGELPELDLPLDYARPPVQSFKGDTIRFRTGSETAK
                     AVEKLLAETGTTLHMVLHAVFHVFLSKISGQRDIVIGSVTAGRTNADVQDMPGMFVNTL
                     ALRMEA"
     CDS_motif       complement(665..754)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20910_0008"
                     /evalue="1.20E-14"
                     /label="C5_LCL_267-296"
                     /locus_tag="PO770_RS20910"
                     /protein_end="889"
                     /protein_start="859"
                     /score="46.6"
                     /tool="antismash"
                     /translation="DIVIGSVTAGRTNADVQDMPGMFVNTLALR"
     CDS_motif       complement(1013..1045)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20910_0007"
                     /evalue="1.30E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="PO770_RS20910"
                     /protein_end="773"
                     /protein_start="762"
                     /score="14.5"
                     /tool="antismash"
                     /translation="HYKDFAVWQNE"
     CDS_motif       complement(1076..1141)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20910_0006"
                     /evalue="2.00E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="PO770_RS20910"
                     /protein_end="752"
                     /protein_start="730"
                     /score="29.9"
                     /tool="antismash"
                     /translation="MHHIIADGVSRGIFVKELALLY"
     CDS_motif       complement(1337..1447)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20910_0005"
                     /evalue="2.60E-16"
                     /label="C2_LCL_024-062"
                     /locus_tag="PO770_RS20910"
                     /protein_end="665"
                     /protein_start="628"
                     /score="52.0"
                     /tool="antismash"
                     /translation="SYNMPSVLLMEGELDISRLRYSLNQLVNRHESLRTSF"
     aSDomain        complement(1565..1768)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20910_PCP.1"
                     /evalue="1.80E-25"
                     /label="PO770_RS20910_PCP.1"
                     /locus_tag="PO770_RS20910"
                     /protein_end="589"
                     /protein_start="521"
                     /score="80.9"
                     /tool="antismash"
                     /translation="EESLCRIWQKTLGIEAIGIDDNFFDLGGHSLKGMMLIANIQAALE
                     KSVPLKALFEQPTVRQLAAYMEA"
     aSModule        complement(1565..4570)
                     /complete
                     /domains="nrpspksdomains_PO770_RS20915_Condensation_LCL.1"
                     /domains="nrpspksdomains_PO770_RS20910_AMP-binding.1"
                     /domains="nrpspksdomains_PO770_RS20910_PCP.1"
                     /locus_tags="PO770_RS20910"
                     /locus_tags="PO770_RS20915"
                     /monomer_pairings="Asp -> Asp"
                     /monomer_pairings="Asp -> Asp"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(2057..2122)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20910_0004"
                     /evalue="3.80E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="PO770_RS20910"
                     /protein_end="425"
                     /protein_start="403"
                     /score="38.1"
                     /tool="antismash"
                     /translation="FIGRADDQVKIRGYRIEPKEIE"
     aSDomain        complement(2087..3295)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20910_AMP-binding.1"
                     /evalue="3.10E-116"
                     /label="PO770_RS20910_AMP-binding.1"
                     /locus_tag="PO770_RS20910"
                     /protein_end="415"
                     /protein_start="12"
                     /score="380.3"
                     /specificity="substrate consensus: Asp"
                     /tool="antismash"
                     /translation="FEKQAQQTPDHPAVKAGGNLLTYRELDEQANQLAHHLRAQGAGNE
                     DIVAIVMDRSAEVMVSILGVMKAGAAFLPIDPDTPEERIRYSLEDSGAKIAVVNERNMT
                     AIGQYEGTIVSLDDGKWRNESKERPSSISGSRNLAYVIYTSGTTGKPKGVQIEHRNLTN
                     YVSWFSEEASLTENDKTVLLSSYAFDLGYTSMFPVLLGGGELHIVQKETYTAPDEIAHY
                     IKEHGITYIKLTPSLFHTIVNTASFAKDANFESLRLIVLGGEKIIPTDVLAFRKMYGHT
                     EFINHYGPTEATIGAIAGRVDLSEPDAFAKRPTIGRPIANAGALVLNEALKLVPPGASG
                     QLYITGQGLARGYLNRPQLTAERFVENPYLPGSLMYKTGDVVRRLSDGTLAFIGRADDQ
                     VKIR"
     CDS_motif       complement(2204..2293)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20910_0003"
                     /evalue="4.80E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="PO770_RS20910"
                     /protein_end="376"
                     /protein_start="346"
                     /score="50.8"
                     /tool="antismash"
                     /translation="PPGASGQLYITGQGLARGYLNRPQLTAERF"
     CDS_motif       complement(2822..2881)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20910_0002"
                     /evalue="1.10E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="PO770_RS20910"
                     /protein_end="170"
                     /protein_start="150"
                     /score="33.3"
                     /tool="antismash"
                     /translation="AYVIYTSGTTGKPKGVQIEH"
     CDS_motif       complement(3068..3109)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20910_0001"
                     /evalue="3.80E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="PO770_RS20910"
                     /protein_end="88"
                     /protein_start="74"
                     /score="16.9"
                     /tool="antismash"
                     /translation="LGVMKAGAAFLPID"
     gene            complement(<3404..>4576)
                     /locus_tag="PO770_RS20915"
                     /old_locus_tag="PO770_20920"
                     /pseudo=""
     CDS             complement(<3404..4576)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (2-264). E-value:
                     1.1e-94. Score: 308.8. Matches aSDomain:
                     nrpspksdomains_PO770_RS20915_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="PO770_RS20915"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="PO770_20920"
                     /product="condensation domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 3.8e-86, bitscore:
                     277.2, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MANTSYNVPAVLLLEGEVDKDRLENAIQQLINRHEILRTSFDMID
                     GEVVQTVHKNISFQLAAAKGREEDAEEIIKAFVQPFELNRAPLVRSKLVQLEEKRHLLL
                     IDMHHIITDGSSTGILIGDLAKIYQGADLELPQIHYKDYAVWHKEQTNYQKDEEYWLDV
                     FKGELPILDLPADFERPAERSFAGERVMFGLDKQITAQIKSLMAETDTTMYMFLLAAFN
                     VLLSKYASQDDIIVGSPTAGRTHPDLQGVPGMFVNTVALRTAPAGDKTFAQFLEEVKTA
                     SLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVMFNMQNMEIPSLRLGDLKISSYSMLH
                     HVAKFDLSLEAVEREEDIGLSFDYATALFQDETIRRWSRHFVNIIKAAAAN"
     CDS_motif       complement(3512..3559)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20915_0006"
                     /evalue="1.00E+01"
                     /label="C3_dual_135-156"
                     /locus_tag="PO770_RS20915"
                     /protein_end="355"
                     /protein_start="339"
                     /score="-0.4"
                     /tool="antismash"
                     /translation="HHVAKFDLSLEAVERE"
     CDS_motif       complement(3623..3736)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20915_0005"
                     /evalue="7.60E-17"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="PO770_RS20915"
                     /protein_end="318"
                     /protein_start="280"
                     /score="53.6"
                     /tool="antismash"
                     /translation="ASLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVMFNM"
     aSDomain        complement(3785..4570)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20915_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.10E-94"
                     /label="PO770_RS20915_Condensation_LCL.1"
                     /locus_tag="PO770_RS20915"
                     /protein_end="264"
                     /protein_start="2"
                     /score="308.8"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIITDG"
                     /tool="antismash"
                     /translation="NTSYNVPAVLLLEGEVDKDRLENAIQQLINRHEILRTSFDMIDGE
                     VVQTVHKNISFQLAAAKGREEDAEEIIKAFVQPFELNRAPLVRSKLVQLEEKRHLLLID
                     MHHIITDGSSTGILIGDLAKIYQGADLELPQIHYKDYAVWHKEQTNYQKDEEYWLDVFK
                     GELPILDLPADFERPAERSFAGERVMFGLDKQITAQIKSLMAETDTTMYMFLLAAFNVL
                     LSKYASQDDIIVGSPTAGRTHPDLQGVPGMFVNTVALRTA"
     CDS_motif       complement(3791..3880)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20915_0004"
                     /evalue="3.00E-16"
                     /label="C5_LCL_267-296"
                     /locus_tag="PO770_RS20915"
                     /protein_end="262"
                     /protein_start="232"
                     /score="51.7"
                     /tool="antismash"
                     /translation="DIIVGSPTAGRTHPDLQGVPGMFVNTVALR"
     CDS_motif       complement(4127..4162)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20915_0003"
                     /evalue="4.10E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="PO770_RS20915"
                     /protein_end="150"
                     /protein_start="138"
                     /score="13.1"
                     /tool="antismash"
                     /translation="HYKDYAVWHKEQ"
     CDS_motif       complement(4193..4258)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20915_0002"
                     /evalue="2.80E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="PO770_RS20915"
                     /protein_end="128"
                     /protein_start="106"
                     /score="29.5"
                     /tool="antismash"
                     /translation="MHHIITDGSSTGILIGDLAKIY"
     CDS_motif       complement(4454..4567)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20915_0001"
                     /evalue="5.20E-18"
                     /label="C2_LCL_024-062"
                     /locus_tag="PO770_RS20915"
                     /protein_end="41"
                     /protein_start="3"
                     /score="57.4"
                     /tool="antismash"
                     /translation="TSYNVPAVLLLEGEVDKDRLENAIQQLINRHEILRTSF"
     gene            complement(4522..4662)
                     /gene="comS"
                     /locus_tag="PO770_RS20920"
                     /old_locus_tag="PO770_20925"
     CDS             complement(4522..4662)
                     /codon_start=1
                     /gene="comS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388232.1"
                     /locus_tag="PO770_RS20920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_20925"
                     /product="competence protein ComS"
                     /protein_id="WP_010886404.1"
                     /transl_table=11
                     /translation="MNRSGKHLISSIILYPRPSGECISSISLDKQTQATTSPLYFCWRE
                     K"
     gene            complement(<4754..7798)
                     /gene="srfAB"
                     /locus_tag="PO770_RS20925"
                     /old_locus_tag="PO770_20930"
                     /pseudo=""
     CDS             complement(<4754..7798)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (0-29). E-value: 1.4e-14.
                     Score: 45.0. Matches aSDomain:
                     nrpspksdomains_PO770_RS20925_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (6-305). E-value:
                     9.2e-114. Score: 371.5. Matches aSDomain:
                     nrpspksdomains_PO770_RS20925_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (470-864). E-value:
                     9.6e-113. Score: 368.8. Matches aSDomain:
                     nrpspksdomains_PO770_RS20925_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (970-1013). E-value: 7.4e-14. Score:
                     43.7. Matches aSDomain: nrpspksdomains_PO770_RS20925_PCP.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="PO770_RS20925"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_20930"
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 1.5e-90, bitscore:
                     291.6, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 9.9e-118, bitscore:
                     381.6, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 5e-09, bitscore:
                     25.1, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKKSIQKVYALTPMQEGMLYHAMLDPHSSSYFTQLELGIHGAFD
                     LEIFEKSVNELIRSYDILRTVFVHQQLQKPRQVVLAERITKVHYEDISYADENRQKEHI
                     ERYKQDVQRQGFNLAKDILFKVAVFRLDADQLYLVWSNHHIMMDGWSMGVLMKSLFQNY
                     EALRAGRTPANGQGKPYSDYIKWLGKQDNEEAESYWSERLAGFEQPSVLPGRLPVKKDE
                     YVNKEYSFTWDETLVARIQQTANLHQVTGPNLFQAVWGIVLSKYNFTDDVVFGTVVSGR
                     PSEINGIETMAGLFINTIPVRVKVERDAAFTDIFSAVQQHAVEAERYDYVPLYEIQKRS
                     ALDSNLLNHLVAFENYPLDQELENGSMEDRLGFSIKVESAFEQTSFDFNLIVYPGKTWT
                     VKIKYNGAAFDSAFIERTAEHLTRMMEAAVDQPAAFVREYGLVGDEEQRQIVEVFNSTK
                     AELPEGMAVHQVFEEQAKRTPASTAVVYEGTELTYRELNATANRLARKLVEHGLQKGET
                     AAIMNDRSVETVVGMLAVLKAGAVYVPLDPALPGDRLRFMAEDSSVRMVLTGNSYTGQA
                     HQLQVPVLTLDIGFEESEAADNLNLPSAPSDLAYIMYTSGSTGKPKGVMIEHKSILRLV
                     KNARYVPVTEEDRMAQTGAVSFDAGTFEVFGALLNGAALYPVKKETLLDAKQFAAFLRE
                     QSITTMWLTSPLFNQLAAKDAGMFGTLRHLIIGGDALVPHIVSKVKQASPSLSLWNGYG
                     PTENTTFSTSFLIDREYGGSIPIGKPIGNSTAYILDEQQCLQPIGAPGELCVGGIGVAR
                     GYVNLPELTEKQFLEDPFRPGERIYRTGDLARWLPDGNIEFLGRIDNQVKVRGFRIELG
                     EIETKLNMAEHVTEAAVIIRKNKADENEICAYFTADREVAVSELRKTLSQSLPDYMVPA
                     HLIQMDSLPLTPNGKINKKELPAPQSEAVQPEYAAPETESEKKLAEIWEGILGVRAGVT
                     DNFFMIGGHSLKAMMMTAKIQEHFHK"
     aSDomain        complement(4760..4888)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20925_PCP.1"
                     /evalue="7.40E-14"
                     /label="PO770_RS20925_PCP.1"
                     /locus_tag="PO770_RS20925"
                     /protein_end="1013"
                     /protein_start="970"
                     /score="43.7"
                     /tool="antismash"
                     /translation="EKKLAEIWEGILGVRAGVTDNFFMIGGHSLKAMMMTAKIQEHF"
     aSModule        complement(4760..7780)
                     /complete
                     /domains="nrpspksdomains_PO770_RS20925_Condensation_DCL.1"
                     /domains="nrpspksdomains_PO770_RS20925_AMP-binding.1"
                     /domains="nrpspksdomains_PO770_RS20925_PCP.1"
                     /locus_tags="PO770_RS20925"
                     /monomer_pairings="Val -> Val"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(5177..5242)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20925_0010"
                     /evalue="1.40E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="PO770_RS20925"
                     /protein_end="874"
                     /protein_start="852"
                     /score="39.4"
                     /tool="antismash"
                     /translation="FLGRIDNQVKVRGFRIELGEIE"
     aSDomain        complement(5207..6388)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20925_AMP-binding.1"
                     /evalue="9.60E-113"
                     /label="PO770_RS20925_AMP-binding.1"
                     /locus_tag="PO770_RS20925"
                     /protein_end="864"
                     /protein_start="470"
                     /score="368.8"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="FEEQAKRTPASTAVVYEGTELTYRELNATANRLARKLVEHGLQKG
                     ETAAIMNDRSVETVVGMLAVLKAGAVYVPLDPALPGDRLRFMAEDSSVRMVLTGNSYTG
                     QAHQLQVPVLTLDIGFEESEAADNLNLPSAPSDLAYIMYTSGSTGKPKGVMIEHKSILR
                     LVKNARYVPVTEEDRMAQTGAVSFDAGTFEVFGALLNGAALYPVKKETLLDAKQFAAFL
                     REQSITTMWLTSPLFNQLAAKDAGMFGTLRHLIIGGDALVPHIVSKVKQASPSLSLWNG
                     YGPTENTTFSTSFLIDREYGGSIPIGKPIGNSTAYILDEQQCLQPIGAPGELCVGGIGV
                     ARGYVNLPELTEKQFLEDPFRPGERIYRTGDLARWLPDGNIEFLGRIDNQVKVR"
     CDS_motif       complement(5324..5413)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20925_0009"
                     /evalue="2.70E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="PO770_RS20925"
                     /protein_end="825"
                     /protein_start="795"
                     /score="48.4"
                     /tool="antismash"
                     /translation="PIGAPGELCVGGIGVARGYVNLPELTEKQF"
     CDS_motif       complement(5915..5974)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20925_0008"
                     /evalue="1.80E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="PO770_RS20925"
                     /protein_end="628"
                     /protein_start="608"
                     /score="35.6"
                     /tool="antismash"
                     /translation="AYIMYTSGSTGKPKGVMIEH"
     CDS_motif       complement(6161..6202)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20925_0007"
                     /evalue="4.50E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="PO770_RS20925"
                     /protein_end="546"
                     /protein_start="532"
                     /score="19.7"
                     /tool="antismash"
                     /translation="LAVLKAGAVYVPLD"
     CDS_motif       complement(6728..6844)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20925_0006"
                     /evalue="3.60E-17"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="PO770_RS20925"
                     /protein_end="357"
                     /protein_start="318"
                     /score="54.8"
                     /tool="antismash"
                     /translation="QQHAVEAERYDYVPLYEIQKRSALDSNLLNHLVAFENYP"
     aSDomain        complement(6884..7780)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20925_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="9.20E-114"
                     /label="PO770_RS20925_Condensation_DCL.1"
                     /locus_tag="PO770_RS20925"
                     /protein_end="305"
                     /protein_start="6"
                     /score="371.5"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIMMDG"
                     /tool="antismash"
                     /translation="QKVYALTPMQEGMLYHAMLDPHSSSYFTQLELGIHGAFDLEIFEK
                     SVNELIRSYDILRTVFVHQQLQKPRQVVLAERITKVHYEDISYADENRQKEHIERYKQD
                     VQRQGFNLAKDILFKVAVFRLDADQLYLVWSNHHIMMDGWSMGVLMKSLFQNYEALRAG
                     RTPANGQGKPYSDYIKWLGKQDNEEAESYWSERLAGFEQPSVLPGRLPVKKDEYVNKEY
                     SFTWDETLVARIQQTANLHQVTGPNLFQAVWGIVLSKYNFTDDVVFGTVVSGRPSEING
                     IETMAGLFINTIPVRVKV"
     CDS_motif       complement(6893..6988)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20925_0005"
                     /evalue="7.30E-20"
                     /label="C5_DCL_263-294"
                     /locus_tag="PO770_RS20925"
                     /protein_end="302"
                     /protein_start="270"
                     /score="63.4"
                     /tool="antismash"
                     /translation="DVVFGTVVSGRPSEINGIETMAGLFINTIPVR"
     CDS_motif       complement(7226..7264)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20925_0004"
                     /evalue="1.20E-03"
                     /label="C4_DCL_171-183"
                     /locus_tag="PO770_RS20925"
                     /protein_end="191"
                     /protein_start="178"
                     /score="11.5"
                     /tool="antismash"
                     /translation="PYSDYIKWLGKQD"
     CDS_motif       complement(7310..7375)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20925_0003"
                     /evalue="1.10E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="PO770_RS20925"
                     /protein_end="163"
                     /protein_start="141"
                     /score="37.0"
                     /tool="antismash"
                     /translation="NHHIMMDGWSMGVLMKSLFQNY"
     CDS_motif       complement(7592..7708)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20925_0002"
                     /evalue="2.80E-14"
                     /label="C2_DCL_024-062"
                     /locus_tag="PO770_RS20925"
                     /protein_end="69"
                     /protein_start="30"
                     /score="45.3"
                     /tool="antismash"
                     /translation="SYFTQLELGIHGAFDLEIFEKSVNELIRSYDILRTVFVH"
     aSDomain        complement(7712..7798)
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20925_NRPS-COM_Nterm.1"
                     /evalue="1.40E-14"
                     /label="PO770_RS20925_NRPS-COM_Nterm.1"
                     /locus_tag="PO770_RS20925"
                     /protein_end="29"
                     /protein_start="0"
                     /score="45.0"
                     /tool="antismash"
                     /translation="MSKKSIQKVYALTPMQEGMLYHAMLDPHS"
     CDS_motif       complement(7733..7771)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20925_0001"
                     /evalue="2.30E-05"
                     /label="C1_dual_004-017"
                     /locus_tag="PO770_RS20925"
                     /protein_end="22"
                     /protein_start="9"
                     /score="17.0"
                     /tool="antismash"
                     /translation="YALTPMQEGMLYH"
     CDS             complement(7811..>9938)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (165-233). E-value: 4.1e-28. Score:
                     89.4. Matches aSDomain: nrpspksdomains_PO770_RS20930_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (243-546). E-value:
                     4.8e-180. Score: 592.0. Matches aSDomain:
                     nrpspksdomains_PO770_RS20930_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (551-705). E-value: 8.5e-56.
                     Score: 180.0. Matches aSDomain:
                     nrpspksdomains_PO770_RS20930_TIGR01720.1"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388230.2"
                     /locus_tag="PO770_RS20930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_20935"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_174246774.1"
                     /sec_met_domain="AMP-binding (E-value: 4.3e-31, bitscore:
                     96.2, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 3.3e-19, bitscore:
                     57.7, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 3.2e-98, bitscore:
                     316.9, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LCVAGRGLARGYLNREDETAKRFVADPFVPGERMYRTGDLVKWTG
                     GIEYIGRIDQQVKVRGYRIELSEIEVQLAQLSEVQDAAVTAVKDKGGNTAIAAYVTPES
                     ADIEALKSALKETLPDYMIPGFWVTLDELPVTANGKVDRKALPEPDIEAGSGEYKAPTT
                     DMEELLAGIWQDVLGMSEVGVTDNFFSLGGDSIKGIQMASRLNQHGWKLEMKDLFQHPT
                     IEELTQYVERAEGKQADQGPVEGEVILTPIQRWFFEKNFTNKHHWNQSVMLYAAKGFDP
                     ERVEKTLQALIEHHDALRMVYREGQEDVIQYNRGLEAASAQLEVIQIEGQAADYEDRIE
                     REAERLQSSIDLQEGGLLKAGLFQAEDGDHLLLAIHHLVVDGVSWRILLEDFAAVYTQL
                     EQGNEPVLPQKTHSFAEYAERLQDFANSKAFLKEKEYWRQLEEQAVAAKLPKDRESGDQ
                     RMKHTKTIEFSLTAEETEQLTTKVHEAYHTEMNDILLTAFGLAMKEWTGQDRVSVHLEG
                     HGREEIIEDLTISRTVGWFTSMYPMVLDMKHADDLGYQLKQMKEDIRHVPNKGVGYGIL
                     RYLTAPEHKEDVAFSIQPDVSFNYLGQFDEMSDAGLFTRSELPSGQSLSPETEKPNALD
                     VVGYIENGKLTMSLAYHSLEFHEKTVQTFSDSFKAHLLRIIEHCLSQDGTELTPSDLGD
                     DDLTLDELDKLMEIF"
     gene            complement(7811..>9942)
                     /locus_tag="PO770_RS20930"
                     /old_locus_tag="PO770_20935"
     aSDomain        complement(7823..8284)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20930_TIGR01720.1"
                     /evalue="8.50E-56"
                     /label="PO770_RS20930_TIGR01720.1"
                     /locus_tag="PO770_RS20930"
                     /protein_end="705"
                     /protein_start="551"
                     /score="180.0"
                     /tool="antismash"
                     /translation="LGYQLKQMKEDIRHVPNKGVGYGILRYLTAPEHKEDVAFSIQPDV
                     SFNYLGQFDEMSDAGLFTRSELPSGQSLSPETEKPNALDVVGYIENGKLTMSLAYHSLE
                     FHEKTVQTFSDSFKAHLLRIIEHCLSQDGTELTPSDLGDDDLTLDELDKL"
     aSModule        complement(7823..9442)
                     /domains="nrpspksdomains_PO770_RS20930_PCP.1"
                     /domains="nrpspksdomains_PO770_RS20930_Epimerization.1"
                     /domains="nrpspksdomains_PO770_RS20930_TIGR01720.1"
                     /incomplete
                     /locus_tags="PO770_RS20930"
                     /tool="antismash"
                     /type="unknown"
     CDS_motif       complement(8201..8260)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20930_0007"
                     /evalue="1.30E-09"
                     /label="NRPS-E6"
                     /locus_tag="PO770_RS20930"
                     /protein_end="579"
                     /protein_start="559"
                     /score="30.3"
                     /tool="antismash"
                     /translation="KEDIRHVPNKGVGYGILRYL"
     aSDomain        complement(8300..9208)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20930_Epimerization.1"
                     /evalue="4.80E-180"
                     /label="PO770_RS20930_Epimerization.1"
                     /locus_tag="PO770_RS20930"
                     /protein_end="546"
                     /protein_start="243"
                     /score="592.0"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="EGEVILTPIQRWFFEKNFTNKHHWNQSVMLYAAKGFDPERVEKTL
                     QALIEHHDALRMVYREGQEDVIQYNRGLEAASAQLEVIQIEGQAADYEDRIEREAERLQ
                     SSIDLQEGGLLKAGLFQAEDGDHLLLAIHHLVVDGVSWRILLEDFAAVYTQLEQGNEPV
                     LPQKTHSFAEYAERLQDFANSKAFLKEKEYWRQLEEQAVAAKLPKDRESGDQRMKHTKT
                     IEFSLTAEETEQLTTKVHEAYHTEMNDILLTAFGLAMKEWTGQDRVSVHLEGHGREEII
                     EDLTISRTVGWFTSMYPMVLDM"
     CDS_motif       complement(8306..8353)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20930_0006"
                     /evalue="1.30E-08"
                     /label="NRPS-E5"
                     /locus_tag="PO770_RS20930"
                     /protein_end="544"
                     /protein_start="528"
                     /score="26.8"
                     /tool="antismash"
                     /translation="ISRTVGWFTSMYPMVL"
     CDS_motif       complement(8762..8827)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20930_0005"
                     /evalue="2.40E-12"
                     /label="NRPS-E2"
                     /locus_tag="PO770_RS20930"
                     /protein_end="392"
                     /protein_start="370"
                     /score="38.9"
                     /tool="antismash"
                     /translation="LLLAIHHLVVDGVSWRILLEDF"
     CDS_motif       complement(9029..9103)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20930_0004"
                     /evalue="2.60E-06"
                     /label="C2_LCL_024-062"
                     /locus_tag="PO770_RS20930"
                     /protein_end="303"
                     /protein_start="278"
                     /score="19.8"
                     /tool="antismash"
                     /translation="FDPERVEKTLQALIEHHDALRMVYR"
     CDS_motif       complement(9164..9193)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20930_0003"
                     /evalue="4.50E-05"
                     /label="NRPS-E1"
                     /locus_tag="PO770_RS20930"
                     /protein_end="258"
                     /protein_start="248"
                     /score="16.1"
                     /tool="antismash"
                     /translation="LTPIQRWFFE"
     aSDomain        complement(9239..9442)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20930_PCP.1"
                     /evalue="4.10E-28"
                     /label="PO770_RS20930_PCP.1"
                     /locus_tag="PO770_RS20930"
                     /protein_end="233"
                     /protein_start="165"
                     /score="89.4"
                     /tool="antismash"
                     /translation="EELLAGIWQDVLGMSEVGVTDNFFSLGGDSIKGIQMASRLNQHGW
                     KLEMKDLFQHPTIEELTQYVERA"
     CDS_motif       complement(9728..9793)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20930_0002"
                     /evalue="1.40E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="PO770_RS20930"
                     /protein_end="70"
                     /protein_start="48"
                     /score="36.3"
                     /tool="antismash"
                     /translation="YIGRIDQQVKVRGYRIELSEIE"
     CDS_motif       complement(9869..>9936)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20930_0001"
                     /evalue="4.90E-11"
                     /label="NRPS-A_a6"
                     /locus_tag="PO770_RS20930"
                     /protein_end="23"
                     /protein_start="0"
                     /score="34.9"
                     /tool="antismash"
                     /translation="LCVAGRGLARGYLNREDETAKRF"
ORIGIN
        1 gcttcgtgag aatgccgacg cggtcatccg ggctgatgcc gcagtccatc agaatccgcg
       61 cgagacggtt tgccttcgca ttcagctcgc cgtacgtcca agactggccg ttgtatgtga
      121 cagccggaca gtctttgtgg cactggacag tctcttcgaa tagctgatga accgttttgt
      181 ctgtcggcac aggcagcgct ttgcccttcc atgcctcaag aagtgcttgc ttttctgtct
      241 ctgtgacaag tgacaggctg gaaagcggct ggttcgggtt atccgcaaca gcctttagta
      301 gccgcagaac gtattcgctc catttttcaa ctgtttcttt cgcgaacaga tctgtcgcat
      361 attcgagctg caagccaatg ccattttcat cagtaaatcc gcccagtgtc agatcaaact
      421 tagaagttcc ctgatgagcc tcataaggtg aaatgctcag attttgcaat gtaagctgtt
      481 ctttatcagg gttttctgtc gtcaccatca cattaaacaa tgggtttcgg ctcatatccc
      541 gagggagatc aagctgatta accagatctt caaacggata ctcctgatgc tcaagcgctg
      601 acaggttcgt ctgctttgct tgctccaaaa gctccgcaaa tgtttgctgt tctttcgctt
      661 ccattctcag ggcaagtgta ttgacgaaca ttcccggcat gtcctgaaca tcagcattcg
      721 tccggccggc agtaacggag ccgatgacga tatcccgctg tccggaaatt ttgcttaaaa
      781 agacgtggaa aacagcatgg agcaccatgt gcaaggtcgt tccggtttcc gcaagcagtt
      841 tttctaccgc ctttgccgtc tcacttcccg tacggaaacg gatcgtatct cctttaaagc
      901 tttgcacagg cggacgggca taatcgagcg ggagatcaag ctctggcagc tcgccagaaa
      961 gaaccgacat ccaatacgcc tcatgctcct tcatccgttc tttttgctca gcttcatttt
     1021 gccaaacggc gaaatcttta taatgaagcg ccggctccgg aagctgttct cctttgtaaa
     1081 gcagcgccaa ttcttttaca aaaatgcctc ttgagacgcc gtctgcgatg atgtgatgca
     1141 tatctaaaag cagcaaatgt tttttcgctt ctattcgaag caaagctgcg cgaatgagcg
     1201 gtgcgtcgtt taagtcgaat ggccggataa attccttaat cttttggtcc gcttcgtctt
     1261 ctttggcttc aaacacatga agatcaacct ccgccttctc aatgatgcgc tgaacaggct
     1321 caccattagc ttccataaat gatgtccgca atgattcgtg acggttcaca agctgattga
     1381 gtgagtaacg caggcgcgaa atatcaagct ctccttccat tagaagaaca gatggcatgt
     1441 tgtagcttat cgtctggcgg tcaagctgat tgagcacgta catccgtttc tgggcggatg
     1501 acaatggata catagcctgc ttgtcagccg gtttgaggac ttgatggccg cctgaaacgg
     1561 cagacgcctc catataagcc gccagctggc gaacagtcgg ctgctcgaac agtgctttaa
     1621 gcggtacgct tttctccaat gccgcctgaa tgttggcaat cagcatcatc ccttttaatg
     1681 aatggccgcc taaatcgaaa aaattgtcat caatgccgat ggcttctatg ccaagcgttt
     1741 tctgccaaat gcggcataag ctttcttcga gcgctgtctc cggtgcggcc aaggctttgc
     1801 ctcctgattg tgccgcgttc ggcttcggca aagcgtttct gtcggttttt ccgtttgccg
     1861 tcagcggaat cgcgtcaacc tgcacaaaga aagcaggaat catatgagac ggcagtgtta
     1921 gggaaagctg cttccggagc tctgcttttt caatagcttg atccgacgta taatacgcgc
     1981 aaagctcttg aagaccgccc tcggaaaccg ctaatacaac cgcttcttga attccgctga
     2041 ggctgagcat gaccgtttca atttctttcg gctcaatgcg gtagccgcgg attttcacct
     2101 gatcatcagc ccggccgata aatgcaagcg taccgtcaga aagtcttcgt acgacatctc
     2161 cggttttgta catgaggctt cccggcaaat atggattttc tacaaatctc tcggctgtca
     2221 gctgaggcct gttgagatac cctctcgcaa gcccctgtcc cgtgatatag agctgtccgc
     2281 tcgctcccgg cggcacaagc ttcaatgctt catttaagac aagcgcgccg gcattcgcaa
     2341 tcgggcgtcc gattgtcggg cgtttcgcaa atgcatccgg ctcagacaga tcaacccgcc
     2401 cggcgatggc gccgatcgtt gcttctgtcg ggccgtagtg attgataaat tcggtatgtc
     2461 catacatctt acggaaggcg agaacatcag tcgggatgat tttttctcct cccaagacga
     2521 tcaagcgcaa ggattcaaag ttcgcatctt ttgcaaaact ggcggtgttc actattgtat
     2581 ggaacagaga cggtgtcagc ttgatatatg tgatcccatg ctccttgata tagtgcgcta
     2641 tttcatctgg cgccgtatag gtttccttct ggacgatatg gagctcgccc ccgcccagaa
     2701 gtacagggaa catgctcgta tagccaaggt caaatgcgta agatgaaagc aatacagtct
     2761 tatcattttc cgtcaggctc gcctcttcac taaaccaaga gacatagttt gtcagattac
     2821 gatgctcaat ctgcacgccc tttggctttc cggtcgtacc ggacgtataa atgacgtatg
     2881 caagattgcg agacccggaa atggatgatg ggcgctcctt gctttcattt ctccatttac
     2941 cgtcatcaag gctgacgatt gtcccttcat attgcccaat agccgtcatg tttctttcat
     3001 tcacgaccgc aatttttgct ccgctgtcct ctaatgaata acggattcgt tcttcaggtg
     3061 tatcaggatc aatcggaagg aaagctgccc ccgccttcat gacaccgaga atggatacca
     3121 tgacttcagc tgaccggtcc ataacaatcg cgacaatgtc ttcatttccg gccccttggg
     3181 cacgaagatg atgcgccagc tggttcgcct gttcatcaag ctcgcgatag gtcagcagat
     3241 ttccgccagc cttcaccgca ggatggtcag gtgtttgctg ggcctgtttt tcaaaaagag
     3301 cggcaaaggt tgcttcggta atttgagtca tatgtctttc ttctagcaaa gcagccgttt
     3361 ctgcagatga aagcagatct acatcagaca gccgaacgtt cggattagcc gcggccgctt
     3421 tgatgatatt aacaaagtgg cggctccagc ggcggatcgt ctcgtcctga aacaaggcag
     3481 tcgcatagtc aaagcttagg ccgatatcct cttcacgctc gaccgcctcc aaggaaagat
     3541 caaatttcgc aacatgatga agcatggaat acgaggaaat cttcaaatct cctaatctta
     3601 atgaaggaat ctccatattc tgcatgttga acatcacgct gaacagcgga cttctgcttg
     3661 tatcccttgt aagcggaagc ttttcaatca actcctcaag cggatagctc tggtgctcga
     3721 atgcttgaag gctggctgtt ttgacctctt caagaaattg cgcgaaggtt ttatctcccg
     3781 ctggtgccgt tctgagtgcc accgtgttga caaacatacc cggcacacct tgcagatcag
     3841 gatgtgttct gccagctgtc ggcgagccga caatgatatc atcctgtgac gcgtacttgg
     3901 aaaggagtac attgaacgcc gccagcaaaa acatgtacat tgtcgtatct gtttctgcca
     3961 tgagcgattt gatttgagcc gtgatttgct tatcaagccc aaacatcacg cgctctcccg
     4021 caaagctccg ttcagctggc cgctcgaaat ccgcgggaag atccagtatt ggcagttcgc
     4081 ctttaaagac atcgagccag tattcctcat ctttttgata attagtttgt tctttgtgcc
     4141 aaactgcgta atctttatag tgaatttgtg gcagttccag atctgcgcct tgatatattt
     4201 tggcaagatc accgattaga atgcctgttg aacttccgtc agtaataata tgatgcatat
     4261 caatgagcag caggtggcgt ttttcttcca gctggacaag cttcgaacgg acgagcggcg
     4321 cgcggtttaa ttcaaacggc tgaacaaatg ctttgattat ctcttccgcg tcttcttccc
     4381 gtcccttggc agccgccagc tggaacgata tgtttttatg aacggtttgc acaacctctc
     4441 cgtcgatcat gtcaaacgat gtacggagga tttcgtgccg gttgattaat tgctgaatcg
     4501 cgttttcaag ccggtcttta tctacttctc cctccagcag aagtacagcg gggacgttgt
     4561 agcttgtgtt tgcttgtcca agctgattga ggatatacat tctccgctgg gccggggata
     4621 caggataatg ctgctgataa gatgcttgcc tgatcggttc aaacgtctgc tcctccttgc
     4681 tttcgttctc ttccaaatac agtgccagtt cttgaatagt cggcttttca aaaagcactt
     4741 ttatcggaac ttccttatga aaatgctctt gaattttcgc cgtcatcatc atcgctttca
     4801 aagaatggcc gccgatcata aagaagttat cggtaacgcc tgctctgacg ccgagtattc
     4861 cttcccagat ctccgctaat ttcttttcac tctctgtttc tggtgctgcg tattccggct
     4921 gcacggcttc tgattgcggt gcaggcagtt cttttttgtt gattttcccg tttggcgtga
     4981 gcggcagact gtccatttga atcaggtggg cagggaccat atagtcaggc aaagactgag
     5041 acagtgtttt tctcagctcg ctcacagcca cttcacggtc cgccgtaaag tacgcgcaaa
     5101 tttcattttc atccgctttg ttcttgcgga tgatcaccgc agcctctgtc acatgctcag
     5161 ccatgttcag ttttgtttca atttcgccaa gctcaattcg gaagccgcgc accttcactt
     5221 ggttgtcgat tctgcctaaa aattcgatat tgccgtccgg cagccatctt gccaagtcac
     5281 cagtgcgata aattctctca cccggtctga acggatcttc gagaaattgc ttctctgtca
     5341 gctcagggag attgacatac ccacgcgcta caccgattcc gcctacgcaa agctcaccag
     5401 gcgcgccgat tggctgcagg cattgctgct catccaggat gtaggcagtg gaattaccga
     5461 tcggcttccc gattgggata gagccgccat actcgcggtc gatcagaaaa ctggttgaaa
     5521 atgtcgtgtt ttctgtcggg ccgtatccgt tccacaatga aagcgacggc gatgcctgtt
     5581 ttactttgct gacaatatgc gggacaaggg catctccgcc gatgattaaa tggcgcagtg
     5641 tgccgaacat acccgcatcc tttgcagcaa gctggttgaa taaaggtgat gtcagccaca
     5701 tggttgtgat gctttgctca cgcaggaatg cagcaaattg ttttgcatca agcagcgttt
     5761 cttttttgac tggataaagc gctgcgccat tcagcagtgc gccgaatacc tcaaacgttc
     5821 cggcatcaaa gctgacagct cccgtttgcg ccatgcggtc ctcttcagta acaggaacgt
     5881 acctggcatt tttgacgagg cgcagaatgc ttttatgttc gatcatgacg cctttcggtt
     5941 tgcccgttga accggatgtg tacatgatgt acgccaaatc agacggggcg gatggcaggt
     6001 tgagattgtc agccgcttca ctctcttcaa agcctatgtc cagtgtaaga accggcactt
     6061 gcagctgatg tgcctgccct gtataagaat ttccggtcaa aaccattcga acggagctgt
     6121 cctctgccat gaaacgaaga cgatcccccg gaagcgctgg atcaagcggc acatatacgg
     6181 cacctgcttt taacacagcc agcatgccga caacggtttc tactgagcga tcgttcataa
     6241 tcgctgctgt ttcccctttt tgaagcccat gttcgacaag ctttctcgcc agacggttag
     6301 ccgttgcatt cagctcgcgg tacgtcagct cggttccttc atatacgacg gcggtgctcg
     6361 ccggcgtccg tttcgcttgc tcttcaaaca cttggtgaac agccattcct tcagggagtt
     6421 cggctttcgt gctgttaaac acctcgacaa tttgccgctg ctcttcgtct ccaacaagcc
     6481 cgtactcacg cacaaaagcg gccggctgat cgacagctgc ttccatcatg cgggtaaggt
     6541 gctccgctgt ccgttcgata aaagcggaat caaaggcggc tccgttatat ttaattttga
     6601 cggtccacgt tttgcccgga tacacaatca ggttgaaatc gaagctcgtt tgttcaaatg
     6661 cgctttctac cttaattgaa aacccgaggc ggtcttccat gctgccgttt tcaagctctt
     6721 gatcaagcgg ataattttca aacgcgacca gatggtttaa gagattgcta tcgagcgctg
     6781 agcgtttttg aatctcatag agcggcacgt aatcgtaacg ctccgcctct actgcatgct
     6841 gctgaacagc tgagaaaata tcagtgaatg cagcatctcg ttcaactttt acccgcaccg
     6901 gaatggtgtt gataaacagc cccgccatcg tttcaattcc gttgatttca gacggccttc
     6961 ccgagacgac cgttccgaag acaacatcat ccgtaaagtt gtatttgctg agaacaatgc
     7021 cccaaacggc ctgaaatagg ttaggccctg tcacttgatg gagatttgcg gtttgctgaa
     7081 tacgggcaac cagtgtttcg tcccatgtaa atgaatattc tttattgacg tattcgtctt
     7141 ttttcacagg caggcggccc gggagcacgc ttggctgttc aaaacccgcc aagcgctcgc
     7201 tccagtagct ctccgcttct tcattgtcct gttttccaag ccacttgatg taatcggaat
     7261 aaggcttgcc ctgaccgttt gccggtgtcc tgcctgctct gagcgcttca tagttttgga
     7321 acaggctttt catgaggacg cccatgctcc agccgtccat cataatatga tgattgctcc
     7381 agacaagata cagctgatct gcatcaaggc ggaaaaccgc caccttgaac aatatgtctt
     7441 ttgccaggtt aaagccttgg cgctgaacgt cttgtttgta acgctcaatg tgctctttct
     7501 ggcggttctc gtctgcataa ctgatatcct cataatgcac ctttgttatg cgttccgcta
     7561 acacgacttg acgcggtttt tgcagctgct gatgcacaaa taccgtacgg agaatatcgt
     7621 atgaccgaat cagttcattg acgcttttct caaagatttc aagatcaaaa gcgccgtgaa
     7681 tcccaagctc taattgtgtg aagtacgagg aagaatgcgg atcaagcatc gcatgataca
     7741 gcattccctc ctgcattggt gtcagtgcgt acaccttttg aatcgatttt ttgctcatat
     7801 gccacctcta ttagaaaatt tccattaatt tatccagctc atccagcgtc aaatcgtcgt
     7861 caccaagatc actcggggtc agttccgtac cgtcttgaga taggcaatgt tcaatgattc
     7921 tgagaagatg cgctttaaag ctgtcactga atgtttgtac tgttttttca tgaaattcaa
     7981 gagaatgata ggccagtgac atcgtcagtt ttccgttttc gatatatccg acaacatcca
     8041 gcgcattcgg tttttctgtt tcagggctta atgactgtcc tgatggcagc tctgatctcg
     8101 taaacaaacc tgcatccgac atttcgtcaa actgacctaa atagttgaag ctgacatccg
     8161 gctgaatcga gaacgccaca tcttctttat gttccggtgc cgtcagatag cgcagaatgc
     8221 cgtatccgac tcccttattc ggcacatgtc tgatatcttc tttcatttgc ttcagctggt
     8281 agcccagatc atccgcatgc ttcatatcga gcaccattgg gtacatactt gtaaaccagc
     8341 cgactgtgcg ggaaatggtc aggtcttcta tgatttcttc acgtccatgc ccctctaaat
     8401 gaacacttac tcgatcctga cccgtccact ccttcattgc caatccgaat gccgtcagca
     8461 aaatatcatt catttctgtg tgatatgcct catgcacctt tgtagtgagc tgttctgtct
     8521 cttcagcagt cagcgagaat tcaattgtct ttgtatgttt cattcgctga tcaccagatt
     8581 cgcggtcttt cggaagcttt gctgcgactg cttgttcttc aagctgtctc caatactctt
     8641 tttctttcaa aaaggctttg gagttcgcga agtcttgcaa tctctctgca tactctgcaa
     8701 atgaatgtgt tttctgcggg agaaccggtt cattgccttg ctcaagctgt gtatatactg
     8761 cggcgaaatc ctccagtaaa atccgccacg acacgccgtc aaccactaaa tggtgaatgg
     8821 caagaagcaa gtggtctccg tcttccgctt ggaacaagcc tgcttttaac aagccgcctt
     8881 cctgcaagtc gatgctgctt tgtaaacgct ccgcttctct ctctatacgg tcttcgtaat
     8941 ctgcagcttg gccctcaatc tggatgacct ccaattgagc tgaagcagct tcaagacctc
     9001 tgttgtattg aatgacgtct tcctgtccct cacggtagac catacggagc gcgtcatgat
     9061 gctcgatcag cgcctgcaac gttttctcca cccgttcagg atcaaagccc ttcgcggcgt
     9121 aaagcatcac cgattggttc cagtggtgct tgttcgtgaa gttcttttca aagaaccagc
     9181 gctggatcgg cgtcaggatg acttcgccct ccaccgggcc ttggtctgcc tgtttgcctt
     9241 cggcacgctc tacgtactga gtaagctctt cgatcgtcgg gtgctggaag agatctttca
     9301 tttccagctt ccagccgtgc tggttcaagc ggctcgccat ttgaattcct ttgatggaat
     9361 ctccgccaag cgagaagaaa ttgtcggtga caccgacttc agacattcca agcacgtcct
     9421 gccagatgcc ggcaagcagt tcttccatgt cggtcgtcgg cgctttgtat tctccgcttc
     9481 ccgcttcgat gtccggctca ggcaaggctt tgcggtccac tttgccgttt gccgtaaccg
     9541 gaagctcgtc cagcgtcacc cagaaccccg ggatcatgta atccggcagg gtttccttca
     9601 gtgctgattt cagtgcttct atgtcagctg attccggtgt gacatacgcc gcgattgctg
     9661 tattgccgcc tttatccttg acagctgtca ccgccgcatc ctgcacctca gaaagctggg
     9721 cgagctggac ttcaatttct gagagctcga tccggtagcc gcggaccttg acctgctggt
     9781 cgatccggcc gatgtattcg atgccgcctg tccacttcac caaatcgccg gtgcggtaca
     9841 tgcgctcacc cggcacaaac ggatcagcga caaaccgctt cgcggtttcg tcttctcgat
     9901 tcagatagcc tcgtgccagt ccgcgccccg ccacgcaaag ct
//
