LOCUS       NZ_JAQOXM010000009     13117 bp    DNA     linear   CON 10-MAR-2024
DEFINITION  Bacillus subtilis strain CWTS 5 NODE_9_length_13117_cov_24.5972,
            whole genome shotgun sequence.
ACCESSION   NZ_JAQOXM010000009
VERSION     NZ_JAQOXM010000009.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 13117)
  AUTHORS   Natarajan,D.A., Chandwani,S. and Dewala,S.
  TITLE     Complete genome sequencing of Bacillus subtilis (CWTS 5), a
            siderophore-producing bacterium triggers antagonistic potential
            against Ralstonia solanacearum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13117)
  AUTHORS   Natarajan,D.A., Chandwani,S. and Dewala,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2023) Molecular Biology Unit, Uka Tarsadia
            University, Bardoli, India, Maliba campus, Bardoli, Gujarat 394620,
            India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-JUL-2022
            Assembly Method                   :: SPAdes v. 3.11.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 02:26:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,268
            CDSs (total)                      :: 4,150
            Genes (coding)                    :: 4,020
            CDSs (with protein)               :: 4,020
            Genes (RNA)                       :: 118
            rRNAs                             :: 10, 12, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 11, 6 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 77 of 130
            Pseudo Genes (internal stop)      :: 19 of 130
            Pseudo Genes (multiple problems)  :: 23 of 130
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:37:08
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 13117
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAQOXM010000009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..13117
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     gene            complement(<1..1681)
                     /locus_tag="PO770_RS20885"
                     /old_locus_tag="PO770_20890"
     CDS             complement(<1..1681)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (2-30). E-value: 1.2e-16.
                     Score: 51.6. Matches aSDomain:
                     nrpspksdomains_PO770_RS20885_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (7-305). E-value:
                     2.9e-119. Score: 389.6. Matches aSDomain:
                     nrpspksdomains_PO770_RS20885_Condensation_DCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389714.1"
                     /locus_tag="PO770_RS20885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_20890"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_165634645.1"
                     /sec_met_domain="Condensation (E-value: 2.1e-94, bitscore:
                     303.6, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 8.9e-25, bitscore:
                     74.7, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQQPEIQDIYPLSFMQEGMLFHSLYDEQSRAYFEQASFTINGQL
                     DLERFQKSMDAVFDRYDIFRTAFIYKNVAKPRQVVLKQRHCHVHVEDISHLNEKDKEHC
                     TEAFKEQDKSKGFDLQTDVLMRISILKWAPDHYVCIWSHHHILMDGWCLGIVIKDFLHI
                     YQALGKGQFPDLPPVQPYGTYIKWLMQQEREEAAEYWKKRLQHFEKASPLPKRTDQMSD
                     GTLQQITFTIPEKETSELQKIAAACGATLNTVFQALWGIMLQKFNRRDDAVFGSVISGR
                     PSELKDVENMVGLFINTIPIRVQSDFLSFSDLVSGMQKDMTEAEAYSYFPLYDIQAQSA
                     LKQELIDHIIVFENTPTQQEIEELNQAGSFDFSVKDFEMEEVTNYRCSVKVIPGRTLYV
                     RIHFHTGTYQPNMMSEIKDYLQHMISDVISDPSLPVSKMTLLDEDKTRKIVSQNNRTVS
                     VSPEAPTLHGLFERQAAVTPERPAIRFSGGSLTYTELDMYANRLAAHLAARGVTNESIV
                     GILSERSPDMLIAVLAVLKAGGAYLPLDPAYPKERLSYMLKDS"
     source          1..13117
                     /altitude="22 m"
                     /collected_by="Dr. Natarajan Amaresan"
                     /db_xref="taxon:1423"
                     /identified_by="Dr. Natarajan Amaresan"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Bacillus subtilis"
                     /strain="CWTS 5"
                     /submitter_seqid="NODE_9_length_13117_cov_24.5972"
     cand_cluster    1..13117
                     /SMILES="NC(CCCN)C(=O)NC(Cc1ccc(O)cc1)C(=O)NC(C(O)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13117
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:>13113](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..>13113)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS_motif       complement(47..88)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20885_0007"
                     /evalue="3.10E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="PO770_RS20885"
                     /protein_end="545"
                     /protein_start="531"
                     /score="20.1"
                     /tool="antismash"
                     /translation="LAVLKAGGAYLPLD"
     CDS_motif       complement(614..730)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20885_0006"
                     /evalue="6.00E-19"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="PO770_RS20885"
                     /protein_end="356"
                     /protein_start="317"
                     /score="60.5"
                     /tool="antismash"
                     /translation="QKDMTEAEAYSYFPLYDIQAQSALKQELIDHIIVFENTP"
     aSDomain        complement(767..1660)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20885_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="2.90E-119"
                     /label="PO770_RS20885_Condensation_DCL.1"
                     /locus_tag="PO770_RS20885"
                     /protein_end="305"
                     /protein_start="7"
                     /score="389.6"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHILMDG"
                     /tool="antismash"
                     /translation="QDIYPLSFMQEGMLFHSLYDEQSRAYFEQASFTINGQLDLERFQK
                     SMDAVFDRYDIFRTAFIYKNVAKPRQVVLKQRHCHVHVEDISHLNEKDKEHCTEAFKEQ
                     DKSKGFDLQTDVLMRISILKWAPDHYVCIWSHHHILMDGWCLGIVIKDFLHIYQALGKG
                     QFPDLPPVQPYGTYIKWLMQQEREEAAEYWKKRLQHFEKASPLPKRTDQMSDGTLQQIT
                     FTIPEKETSELQKIAAACGATLNTVFQALWGIMLQKFNRRDDAVFGSVISGRPSELKDV
                     ENMVGLFINTIPIRVQS"
     CDS_motif       complement(776..871)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20885_0005"
                     /evalue="4.70E-20"
                     /label="C5_DCL_263-294"
                     /locus_tag="PO770_RS20885"
                     /protein_end="302"
                     /protein_start="270"
                     /score="64.0"
                     /tool="antismash"
                     /translation="DAVFGSVISGRPSELKDVENMVGLFINTIPIR"
     CDS_motif       complement(1106..1144)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20885_0004"
                     /evalue="8.20E-03"
                     /label="C4_DCL_171-183"
                     /locus_tag="PO770_RS20885"
                     /protein_end="192"
                     /protein_start="179"
                     /score="9.1"
                     /tool="antismash"
                     /translation="PYGTYIKWLMQQE"
     CDS_motif       complement(1190..1255)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20885_0003"
                     /evalue="3.40E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="PO770_RS20885"
                     /protein_end="164"
                     /protein_start="142"
                     /score="35.5"
                     /tool="antismash"
                     /translation="HHHILMDGWCLGIVIKDFLHIY"
     CDS_motif       complement(1472..1588)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20885_0002"
                     /evalue="9.20E-18"
                     /label="C2_DCL_024-062"
                     /locus_tag="PO770_RS20885"
                     /protein_end="70"
                     /protein_start="31"
                     /score="56.5"
                     /tool="antismash"
                     /translation="AYFEQASFTINGQLDLERFQKSMDAVFDRYDIFRTAFIY"
     aSDomain        complement(1592..1675)
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20885_NRPS-COM_Nterm.1"
                     /evalue="1.20E-16"
                     /label="PO770_RS20885_NRPS-COM_Nterm.1"
                     /locus_tag="PO770_RS20885"
                     /protein_end="30"
                     /protein_start="2"
                     /score="51.6"
                     /tool="antismash"
                     /translation="QQPEIQDIYPLSFMQEGMLFHSLYDEQS"
     CDS_motif       complement(1610..1651)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20885_0001"
                     /evalue="2.90E-05"
                     /label="C1_DCL_004-017"
                     /locus_tag="PO770_RS20885"
                     /protein_end="24"
                     /protein_start="10"
                     /score="15.8"
                     /tool="antismash"
                     /translation="YPLSFMQEGMLFHS"
     gene            complement(1698..9380)
                     /gene="ppsB"
                     /locus_tag="PO770_RS20890"
                     /old_locus_tag="PO770_20895"
     CDS             complement(1698..9380)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (0-29). E-value: 1.6e-15.
                     Score: 48.0. Matches aSDomain:
                     nrpspksdomains_PO770_RS20890_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (7-309). E-value:
                     1.5e-111. Score: 364.2. Matches aSDomain:
                     nrpspksdomains_PO770_RS20890_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (474-865). E-value:
                     2.4e-110. Score: 360.9. Matches aSDomain:
                     nrpspksdomains_PO770_RS20890_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (970-1036). E-value: 1.8e-23. Score:
                     74.5. Matches aSDomain: nrpspksdomains_PO770_RS20890_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (1054-1341). E-value:
                     2e-103. Score: 337.5. Matches aSDomain:
                     nrpspksdomains_PO770_RS20890_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (1505-1903). E-value:
                     1.7e-118. Score: 387.7. Matches aSDomain:
                     nrpspksdomains_PO770_RS20890_AMP-binding.2"
                     /NRPS_PKS="Domain: PCP (2011-2078). E-value: 4.4e-20.
                     Score: 63.7. Matches aSDomain:
                     nrpspksdomains_PO770_RS20890_PCP.2"
                     /NRPS_PKS="Domain: Epimerization (2088-2392). E-value:
                     6.8e-158. Score: 518.7. Matches aSDomain:
                     nrpspksdomains_PO770_RS20890_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (2403-2553). E-value: 6.5e-54.
                     Score: 173.9. Matches aSDomain:
                     nrpspksdomains_PO770_RS20890_TIGR01720.1"
                     /codon_start=1
                     /gene="ppsB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389715.1"
                     /locus_tag="PO770_RS20890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_20895"
                     /product="plipastatin non-ribosomal peptide synthetase
                     PpsB"
                     /protein_id="WP_128441662.1"
                     /sec_met_domain="Condensation (E-value: 5.1e-96, bitscore:
                     308.9, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 2e-126, bitscore:
                     409.5, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.2e-17, bitscore:
                     52.0, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQSAQIQDIYPLSHMQEGMLFHSLMDFSSKAYIEQTSFTITGNL
                     CVDSFQKSLNLLVSRYDIFRTIFIKEVPDLTGPQQVVLSNRELTVYREDISRLADQEQQ
                     TLIDAFMTKDREKGFDLQKDPLMRLALFDRGDSQYTCVWTHHHIIMDGWCLGIILKEFF
                     SMYDSLKNNSPVQLGSTVPYSRYIEWLGEQDQEETAAYWSEYLKEYGNTASIPRIKRRT
                     ADGNYKADQVSFSLASDMVEKLTEAAQNWGVTLNTLFMSIWGVLLHRYNAADDAVFGSV
                     ISGRPSAIDGIESMVGLFINTVPVRIRSAEGITFSSLVKAVQEDILSSEQHGYYPLYEI
                     QNHSPLKQGLIDHIFVFENYPVQLHQALSVESENDEGALKLSDISMSEQTNYDFNIVIV
                     PGESFYIKFSYNADVYEREEMLRIQGHLKQALDCILTNPDVAVSDINIVPPEEQQVIQS
                     FNETERPYVNKTIPQLFEEQAHKTPEAAALKMGNECWTYRQLQVRANQIAHALIEKGVG
                     SGDIVAVMMGRSMEMPAALLGIWKAGGAYMPLDPHFPAERLSFLLKDSQAAQLLIEEDL
                     ISLIPPSYEGNTITIEHTESYQTEAPNMPPGDLAYLIYTSGTTGRPKGVLVDHHGIANT
                     LQWRREEYGMAEQDISLHLFSYVFDGCVTSLFTPLLSGACVLLTTDDEAKDVLALKRKI
                     ARYKVSHMIIVPSLYRVLLEVMTADDAKSLRIVTFAGEAVTPDLLELSQIICPSAELAN
                     EYGPTENSVATTILRHLNEKERITIGHPIRNTKVFVLHGNQMQPIGAAGELCISGAGLA
                     RGYYKQPELTQKAFSDHPFLEGERLYRTGDAGRFLPDGTIEYIGRFDDQVKIRGYRIEL
                     REIETVLRQAPGVKEAAVLARDVSAEEKELVAYIVPEKGNSLPDLYQHLAGTLPSYMIP
                     ASIINIGRMPLTSSGKLDRFALPEPENNTSVTYMAPRTLIEADLAHIWEDVLNKQQIGI
                     RDDFFQLGGQSLKAAALVSRIHKKLNVELPLSEVFSYPTVESMAVKLMSLKEHAFTQIE
                     PADQRDVYPLSFSQKRLYALHQLADDSTGYNMPAVLELRGNLNRQRLRSVLTELVNRHE
                     ALRTVFVLDRDEPVQIIYPEIAFDLKELEMESEQMLESAIETFIKPFDLSSGPLFRACV
                     ITMGNNRGFLLLDMHHIIADGVSMSTLVQEFTDLYCGKELPALNLHYKDFAVWQQEKHP
                     KELYKKQEAYWLGQLGGSLPTLELPLDKTRPRLPDFSGGTIELNIDKDTADELHRLMAE
                     TGTTLYMILLAVYSILLSKLSGQEDIVVGSPAAGRPHADLERVIGMFVNTLAMRSKPEG
                     HKTFSSYLQDIRHLALTAYEHQDYPFEELADKLFTNREVNRNPLFDAMLVLQSSEDFRF
                     EVPGLSISSVTPKHDISKFDLTLHAEEHPSGIRCRFEYSTALFEEETITQWASYFIELV
                     KGVTADTEMRISDMQLLPAAERRLLLEKMGQYAAYPRNENIVSLFEKQAAKYPEHIAVV
                     CGHSQLTYRDLNEKAERAAAMLIKQGVRTGDIVGLMLDRSPDMIIGVLSILKAGGAYLP
                     IDPEYPKERISFMLNDSGAKLLLTERGQNKPADYTGHILYIDECENDSFPADVNIQEIV
                     TDQPAYVIYTSGTTGQPKGVIVEHRNVISLLKHQNLPFEFNHEDIWTLFHSYCFDFSVW
                     EMFGALLNGGTLVVVSKETARDPHAFRLLLKKERVTVLNQTPTAFYGLMLEDQNHTDHL
                     NIRYVIFGGEALQPGMLQSWNKKYPHTDLINMYGITETTVHVTFKKLSAADIVKNKSNI
                     GRPLSTLQAYVMDAHMNLQPIGVPGELYIGGEGVARGYLNRDELTADRFVSNPYLPGDR
                     LYRTGDLAKRLSNGELEYLGRIDEQVKVRGHRIELGEIQAALLQYPMIKEAAVITRADE
                     QGQTAIYAYMVIKDQQAANISDIRTYLKNALPDFMLPARMIQIDSIPVTVNGKLDQKAL
                     PEPEKHAYTADDISPRNEIETVMAEIWEELLNIDELGVSANFFELGGDSIKALQVCARL
                     KQRGFETTVREMFEHQTLGELSARVRKDVRAIDQGPVEGEITWTPIQQWFFSQSLESHH
                     FNQSVMIYRAERFDEAALRKVLKSLVTHHDALRIVCRHEDGRQVQINRGIDLSDEELYA
                     LELFDVKDSLTEARYTIEEAASRMQEHIRLETGPLLHAGLFRTENGDHLFLTIHHLVVD
                     AVSWRILFEDFSAAYKQAVSGESIKLPQKTDSYLTYSQRIVDYSKSRQVQREAAYWDEC
                     ESRHIQPIPKDNDAASNTFKDTEVIDFELSRHHTELLLTAAHKAYSTEMNDILLTALGL
                     ALQKWTGNNQFKISMEGHGRESYLEDIDISRTVGWFTSIYPVWLDMRDSDHKDKEERLG
                     HLIKQTKDMLHRIPHKGAGYGVLKYISKRWGSQKNSPEISFNYLGQFDQDIQSNAFEVS
                     DIKPGNEISPNWERPYALDISGAVSSGCLNMHIIYNRVQFEEKTIQTFSRHFKQTLENI
                     IEHCTGKENREWSASDFTDEDLTLDELSEIMGAVNKL"
     aSDomain        complement(1722..2171)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20890_TIGR01720.1"
                     /evalue="6.50E-54"
                     /label="PO770_RS20890_TIGR01720.1"
                     /locus_tag="PO770_RS20890"
                     /protein_end="2553"
                     /protein_start="2403"
                     /score="173.9"
                     /tool="antismash"
                     /translation="LGHLIKQTKDMLHRIPHKGAGYGVLKYISKRWGSQKNSPEISFNY
                     LGQFDQDIQSNAFEVSDIKPGNEISPNWERPYALDISGAVSSGCLNMHIIYNRVQFEEK
                     TIQTFSRHFKQTLENIIEHCTGKENREWSASDFTDEDLTLDELSEI"
     aSModule        complement(1722..6218)
                     /complete
                     /domains="nrpspksdomains_PO770_RS20890_Condensation_LCL.1"
                     /domains="nrpspksdomains_PO770_RS20890_AMP-binding.2"
                     /domains="nrpspksdomains_PO770_RS20890_PCP.2"
                     /domains="nrpspksdomains_PO770_RS20890_Epimerization.1"
                     /domains="nrpspksdomains_PO770_RS20890_TIGR01720.1"
                     /locus_tags="PO770_RS20890"
                     /monomer_pairings="Thr -> D-Thr"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(2091..2150)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0025"
                     /evalue="4.90E-08"
                     /label="NRPS-E6"
                     /locus_tag="PO770_RS20890"
                     /protein_end="2430"
                     /protein_start="2410"
                     /score="25.3"
                     /tool="antismash"
                     /translation="TKDMLHRIPHKGAGYGVLKY"
     aSDomain        complement(2205..3116)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20890_Epimerization.1"
                     /evalue="6.80E-158"
                     /label="PO770_RS20890_Epimerization.1"
                     /locus_tag="PO770_RS20890"
                     /protein_end="2392"
                     /protein_start="2088"
                     /score="518.7"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDA"
                     /tool="antismash"
                     /translation="EGEITWTPIQQWFFSQSLESHHFNQSVMIYRAERFDEAALRKVLK
                     SLVTHHDALRIVCRHEDGRQVQINRGIDLSDEELYALELFDVKDSLTEARYTIEEAASR
                     MQEHIRLETGPLLHAGLFRTENGDHLFLTIHHLVVDAVSWRILFEDFSAAYKQAVSGES
                     IKLPQKTDSYLTYSQRIVDYSKSRQVQREAAYWDECESRHIQPIPKDNDAASNTFKDTE
                     VIDFELSRHHTELLLTAAHKAYSTEMNDILLTALGLALQKWTGNNQFKISMEGHGRESY
                     LEDIDISRTVGWFTSIYPVWLDM"
     CDS_motif       complement(2211..2261)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0024"
                     /evalue="5.10E-09"
                     /label="NRPS-E5"
                     /locus_tag="PO770_RS20890"
                     /protein_end="2390"
                     /protein_start="2373"
                     /score="28.0"
                     /tool="antismash"
                     /translation="DISRTVGWFTSIYPVWL"
     CDS_motif       complement(2664..2729)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0023"
                     /evalue="1.60E-11"
                     /label="NRPS-E2"
                     /locus_tag="PO770_RS20890"
                     /protein_end="2239"
                     /protein_start="2217"
                     /score="36.3"
                     /tool="antismash"
                     /translation="LFLTIHHLVVDAVSWRILFEDF"
     CDS_motif       complement(2952..3017)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0022"
                     /evalue="1.60E-03"
                     /label="C2_LCL_024-062"
                     /locus_tag="PO770_RS20890"
                     /protein_end="2143"
                     /protein_start="2121"
                     /score="10.9"
                     /tool="antismash"
                     /translation="RFDEAALRKVLKSLVTHHDALR"
     CDS_motif       complement(3075..3101)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0021"
                     /evalue="2.90E-03"
                     /label="NRPS-E1"
                     /locus_tag="PO770_RS20890"
                     /protein_end="2102"
                     /protein_start="2093"
                     /score="10.8"
                     /tool="antismash"
                     /translation="WTPIQQWFF"
     aSDomain        complement(3147..3347)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20890_PCP.2"
                     /evalue="4.40E-20"
                     /label="PO770_RS20890_PCP.2"
                     /locus_tag="PO770_RS20890"
                     /protein_end="2078"
                     /protein_start="2011"
                     /score="63.7"
                     /tool="antismash"
                     /translation="ETVMAEIWEELLNIDELGVSANFFELGGDSIKALQVCARLKQRGF
                     ETTVREMFEHQTLGELSARVRK"
     CDS_motif       complement(3642..3707)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0020"
                     /evalue="3.10E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="PO770_RS20890"
                     /protein_end="1913"
                     /protein_start="1891"
                     /score="35.3"
                     /tool="antismash"
                     /translation="YLGRIDEQVKVRGHRIELGEIQ"
     aSDomain        complement(3672..4865)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20890_AMP-binding.2"
                     /evalue="1.70E-118"
                     /label="PO770_RS20890_AMP-binding.2"
                     /locus_tag="PO770_RS20890"
                     /protein_end="1903"
                     /protein_start="1505"
                     /score="387.7"
                     /specificity="substrate consensus: Thr"
                     /tool="antismash"
                     /translation="FEKQAAKYPEHIAVVCGHSQLTYRDLNEKAERAAAMLIKQGVRTG
                     DIVGLMLDRSPDMIIGVLSILKAGGAYLPIDPEYPKERISFMLNDSGAKLLLTERGQNK
                     PADYTGHILYIDECENDSFPADVNIQEIVTDQPAYVIYTSGTTGQPKGVIVEHRNVISL
                     LKHQNLPFEFNHEDIWTLFHSYCFDFSVWEMFGALLNGGTLVVVSKETARDPHAFRLLL
                     KKERVTVLNQTPTAFYGLMLEDQNHTDHLNIRYVIFGGEALQPGMLQSWNKKYPHTDLI
                     NMYGITETTVHVTFKKLSAADIVKNKSNIGRPLSTLQAYVMDAHMNLQPIGVPGELYIG
                     GEGVARGYLNRDELTADRFVSNPYLPGDRLYRTGDLAKRLSNGELEYLGRIDEQVKVR"
     CDS_motif       complement(3789..3878)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0019"
                     /evalue="3.10E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="PO770_RS20890"
                     /protein_end="1864"
                     /protein_start="1834"
                     /score="57.8"
                     /tool="antismash"
                     /translation="PIGVPGELYIGGEGVARGYLNRDELTADRF"
     CDS_motif       complement(3993..4016)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0018"
                     /evalue="2.50E+00"
                     /label="NRPS-A_a5"
                     /locus_tag="PO770_RS20890"
                     /protein_end="1796"
                     /protein_start="1788"
                     /score="2.0"
                     /tool="antismash"
                     /translation="YGITETTV"
     CDS_motif       complement(4395..4454)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0017"
                     /evalue="4.50E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="PO770_RS20890"
                     /protein_end="1662"
                     /protein_start="1642"
                     /score="31.4"
                     /tool="antismash"
                     /translation="AYVIYTSGTTGQPKGVIVEH"
     CDS_motif       complement(4638..4679)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0016"
                     /evalue="1.70E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="PO770_RS20890"
                     /protein_end="1581"
                     /protein_start="1567"
                     /score="15.0"
                     /tool="antismash"
                     /translation="LSILKAGGAYLPID"
     CDS_motif       complement(5199..5312)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0015"
                     /evalue="3.90E-13"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="PO770_RS20890"
                     /protein_end="1394"
                     /protein_start="1356"
                     /score="41.7"
                     /tool="antismash"
                     /translation="LALTAYEHQDYPFEELADKLFTNREVNRNPLFDAMLVL"
     aSDomain        complement(5358..6218)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20890_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="2.00E-103"
                     /label="PO770_RS20890_Condensation_LCL.1"
                     /locus_tag="PO770_RS20890"
                     /protein_end="1341"
                     /protein_start="1054"
                     /score="337.5"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIADG"
                     /tool="antismash"
                     /translation="VYPLSFSQKRLYALHQLADDSTGYNMPAVLELRGNLNRQRLRSVL
                     TELVNRHEALRTVFVLDRDEPVQIIYPEIAFDLKELEMESEQMLESAIETFIKPFDLSS
                     GPLFRACVITMGNNRGFLLLDMHHIIADGVSMSTLVQEFTDLYCGKELPALNLHYKDFA
                     VWQQEKHPKELYKKQEAYWLGQLGGSLPTLELPLDKTRPRLPDFSGGTIELNIDKDTAD
                     ELHRLMAETGTTLYMILLAVYSILLSKLSGQEDIVVGSPAAGRPHADLERVIGMFVNTL
                     AMRSKP"
     CDS_motif       complement(5367..5456)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0014"
                     /evalue="8.90E-17"
                     /label="C5_LCL_267-296"
                     /locus_tag="PO770_RS20890"
                     /protein_end="1338"
                     /protein_start="1308"
                     /score="53.4"
                     /tool="antismash"
                     /translation="DIVVGSPAAGRPHADLERVIGMFVNTLAMR"
     CDS_motif       complement(5715..5747)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0013"
                     /evalue="3.60E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="PO770_RS20890"
                     /protein_end="1222"
                     /protein_start="1211"
                     /score="13.2"
                     /tool="antismash"
                     /translation="HYKDFAVWQQE"
     CDS_motif       complement(5778..5843)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0012"
                     /evalue="1.90E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="PO770_RS20890"
                     /protein_end="1201"
                     /protein_start="1179"
                     /score="30.0"
                     /tool="antismash"
                     /translation="MHHIIADGVSMSTLVQEFTDLY"
     CDS_motif       complement(6039..6149)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0011"
                     /evalue="7.80E-17"
                     /label="C2_LCL_024-062"
                     /locus_tag="PO770_RS20890"
                     /protein_end="1114"
                     /protein_start="1077"
                     /score="53.7"
                     /tool="antismash"
                     /translation="YNMPAVLELRGNLNRQRLRSVLTELVNRHEALRTVFV"
     aSDomain        complement(6273..6470)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20890_PCP.1"
                     /evalue="1.80E-23"
                     /label="PO770_RS20890_PCP.1"
                     /locus_tag="PO770_RS20890"
                     /protein_end="1036"
                     /protein_start="970"
                     /score="74.5"
                     /tool="antismash"
                     /translation="EADLAHIWEDVLNKQQIGIRDDFFQLGGQSLKAAALVSRIHKKLN
                     VELPLSEVFSYPTVESMAVKL"
     aSModule        complement(6273..9359)
                     /complete
                     /domains="nrpspksdomains_PO770_RS20890_Condensation_DCL.1"
                     /domains="nrpspksdomains_PO770_RS20890_AMP-binding.1"
                     /domains="nrpspksdomains_PO770_RS20890_PCP.1"
                     /locus_tags="PO770_RS20890"
                     /monomer_pairings="Tyr -> Tyr"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(6756..6821)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0010"
                     /evalue="4.20E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="PO770_RS20890"
                     /protein_end="875"
                     /protein_start="853"
                     /score="34.8"
                     /tool="antismash"
                     /translation="YIGRFDDQVKIRGYRIELREIE"
     aSDomain        complement(6786..7958)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20890_AMP-binding.1"
                     /evalue="2.40E-110"
                     /label="PO770_RS20890_AMP-binding.1"
                     /locus_tag="PO770_RS20890"
                     /protein_end="865"
                     /protein_start="474"
                     /score="360.9"
                     /specificity="substrate consensus: Tyr"
                     /tool="antismash"
                     /translation="FEEQAHKTPEAAALKMGNECWTYRQLQVRANQIAHALIEKGVGSG
                     DIVAVMMGRSMEMPAALLGIWKAGGAYMPLDPHFPAERLSFLLKDSQAAQLLIEEDLIS
                     LIPPSYEGNTITIEHTESYQTEAPNMPPGDLAYLIYTSGTTGRPKGVLVDHHGIANTLQ
                     WRREEYGMAEQDISLHLFSYVFDGCVTSLFTPLLSGACVLLTTDDEAKDVLALKRKIAR
                     YKVSHMIIVPSLYRVLLEVMTADDAKSLRIVTFAGEAVTPDLLELSQIICPSAELANEY
                     GPTENSVATTILRHLNEKERITIGHPIRNTKVFVLHGNQMQPIGAAGELCISGAGLARG
                     YYKQPELTQKAFSDHPFLEGERLYRTGDAGRFLPDGTIEYIGRFDDQVKIR"
     CDS_motif       complement(6903..6992)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0009"
                     /evalue="3.20E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="PO770_RS20890"
                     /protein_end="826"
                     /protein_start="796"
                     /score="48.2"
                     /tool="antismash"
                     /translation="PIGAAGELCISGAGLARGYYKQPELTQKAF"
     CDS_motif       complement(7089..7121)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0008"
                     /evalue="1.20E-01"
                     /label="NRPS-A_a5"
                     /locus_tag="PO770_RS20890"
                     /protein_end="764"
                     /protein_start="753"
                     /score="5.7"
                     /tool="antismash"
                     /translation="EYGPTENSVAT"
     CDS_motif       complement(7494..7553)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0007"
                     /evalue="1.60E-09"
                     /label="NRPS-A_a3"
                     /locus_tag="PO770_RS20890"
                     /protein_end="629"
                     /protein_start="609"
                     /score="29.8"
                     /tool="antismash"
                     /translation="AYLIYTSGTTGRPKGVLVDH"
     CDS_motif       complement(7731..7772)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0006"
                     /evalue="6.80E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="PO770_RS20890"
                     /protein_end="550"
                     /protein_start="536"
                     /score="16.1"
                     /tool="antismash"
                     /translation="LGIWKAGGAYMPLD"
     CDS_motif       complement(8298..8414)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0005"
                     /evalue="3.10E-19"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="PO770_RS20890"
                     /protein_end="361"
                     /protein_start="322"
                     /score="61.4"
                     /tool="antismash"
                     /translation="QEDILSSEQHGYYPLYEIQNHSPLKQGLIDHIFVFENYP"
     aSDomain        complement(8454..9359)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20890_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="1.50E-111"
                     /label="PO770_RS20890_Condensation_DCL.1"
                     /locus_tag="PO770_RS20890"
                     /protein_end="309"
                     /protein_start="7"
                     /score="364.2"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIMDG"
                     /tool="antismash"
                     /translation="QDIYPLSHMQEGMLFHSLMDFSSKAYIEQTSFTITGNLCVDSFQK
                     SLNLLVSRYDIFRTIFIKEVPDLTGPQQVVLSNRELTVYREDISRLADQEQQTLIDAFM
                     TKDREKGFDLQKDPLMRLALFDRGDSQYTCVWTHHHIIMDGWCLGIILKEFFSMYDSLK
                     NNSPVQLGSTVPYSRYIEWLGEQDQEETAAYWSEYLKEYGNTASIPRIKRRTADGNYKA
                     DQVSFSLASDMVEKLTEAAQNWGVTLNTLFMSIWGVLLHRYNAADDAVFGSVISGRPSA
                     IDGIESMVGLFINTVPVRIRS"
     CDS_motif       complement(8463..8558)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0004"
                     /evalue="2.40E-19"
                     /label="C5_DCL_263-294"
                     /locus_tag="PO770_RS20890"
                     /protein_end="306"
                     /protein_start="274"
                     /score="61.7"
                     /tool="antismash"
                     /translation="DAVFGSVISGRPSAIDGIESMVGLFINTVPVR"
     CDS_motif       complement(8883..8948)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0003"
                     /evalue="2.50E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="PO770_RS20890"
                     /protein_end="166"
                     /protein_start="144"
                     /score="35.9"
                     /tool="antismash"
                     /translation="HHHIIMDGWCLGIILKEFFSMY"
     CDS_motif       complement(9174..9287)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0002"
                     /evalue="2.10E-14"
                     /label="C2_DCL_024-062"
                     /locus_tag="PO770_RS20890"
                     /protein_end="69"
                     /protein_start="31"
                     /score="45.7"
                     /tool="antismash"
                     /translation="AYIEQTSFTITGNLCVDSFQKSLNLLVSRYDIFRTIFI"
     aSDomain        complement(9294..9380)
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20890_NRPS-COM_Nterm.1"
                     /evalue="1.60E-15"
                     /label="PO770_RS20890_NRPS-COM_Nterm.1"
                     /locus_tag="PO770_RS20890"
                     /protein_end="29"
                     /protein_start="0"
                     /score="48.0"
                     /tool="antismash"
                     /translation="MAQSAQIQDIYPLSHMQEGMLFHSLMDFS"
     CDS_motif       complement(9309..9350)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20890_0001"
                     /evalue="9.00E-05"
                     /label="C1_DCL_004-017"
                     /locus_tag="PO770_RS20890"
                     /protein_end="24"
                     /protein_start="10"
                     /score="14.5"
                     /tool="antismash"
                     /translation="YPLSHMQEGMLFHS"
     CDS             complement(9405..>13113)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: AMP-binding (178-583). E-value: 2.8e-96.
                     Score: 314.6. Matches aSDomain:
                     nrpspksdomains_PO770_RS20895_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (688-752). E-value: 1e-21. Score:
                     68.9. Matches aSDomain: nrpspksdomains_PO770_RS20895_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (764-1075). E-value:
                     1.3e-148. Score: 488.0. Matches aSDomain:
                     nrpspksdomains_PO770_RS20895_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (1083-1228). E-value: 1.2e-46.
                     Score: 150.3. Matches aSDomain:
                     nrpspksdomains_PO770_RS20895_TIGR01720.1"
                     /codon_start=3
                     /gene="ppsA"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389716.2"
                     /locus_tag="PO770_RS20895"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_20900"
                     /product="plipastatin non-ribosomal peptide synthetase
                     PpsA"
                     /pseudo=""
                     /sec_met_domain="AMP-binding (E-value: 1.4e-106, bitscore:
                     344.1, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1e-15, bitscore:
                     45.8, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 4.6e-89, bitscore:
                     286.1, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFVNTLALRTRPEGGKPFVQYLQEVRETALEAFEHQDYPFEELVD
                     KLELTRDMSRNPVFDAMFILQNVEKQDIDLREIKVRPANFAHHISLFDITLIATEINGS
                     ICCEMEFSTEVFLKATIERWADHFIEFLHAALSTPETSLAQINILSDKEKQKIVFGFNK
                     TQVEFAQKDVPFHHIFEAKAEEIPEHIAVIDNETEISYRLLNERANRLARTLQNRKGPK
                     PTVAVLAKRSIDAIVGVLAVMKAGGVYIPIDSHYPKARIEYILRDSGADILLLQRELKH
                     LISNSPESEMSHIFLDDEGSYEESNCNLNLSPALEEPVYIIYTSGTTGAPKGVIVTYRN
                     FTHAALAWRQIYELDRKPVRLLQIASFSFDVFSGDLARTLTNGGTLIVCPDETRLEPAE
                     IYKIMNSQRITVMESTPALIIPVMEYVYRNQFKLPDLDILILGSDMVKAQDFKTLTDRF
                     GQSMRIINSYGVTEATIDSSFYETSMGGECTGDNVPIGSPLPNVHMYVLSQTDQIQPIG
                     VAGELCIGGAGVAKGYHHKPDLTQMKFTENPFVSGERLYRTGDRACWLPNGSIRLLGRM
                     DYQVKINGYRIETEEIESVLLQTGLVREAAVAVQHDKNGQAGLAAYIVPSDVNTNALRA
                     ALTKELPAYMIPAHLIPLENMPLTLNGKLDRNALPAPNNVLSRPYTAPVNDIQKTMAYI
                     WEDVLSMPRVGIHDSFFELGGDSIKALQVAARLAAEGWSMTIRDLFRYSTIQELCGHIT
                     PLASQADQGPAEGEAELTPIQRRFFGQVHAFHYHYNQSVMLFSEKGFNANALHLALRKI
                     TEHHDAIRMIFQHDQNGHVIQFNRGINHKDHELFGLYISDWTKASLERAHLDEKLAAEE
                     TVIQSKMNVEKGPLLQAGLFKTAEGDHLLIALHHLVIDGVSWRILLEDLAAAYQQALEK
                     KEIQLPPKTDSYLSYADGLTQIAESKQLLSEKPYWQTILDAHTAFLPKDIENVPDRLQM
                     NSDAAAFVLSGDWTEKLLFETQQAYGTDANELLLTALGMALSEWTGHDQIVISTEGHGR
                     EGHVPNIDISRTVGWFTSIYPILLDMGIPEPFEDQLAYRIKTTKDMLRRVPNKGTGYGL
                     LTHIGELRHKEPEVSFNYLGQFSEEKEAETFQLSYYKPRHEIAGKREREYELDINALIT
                     DGRLQVKAVYTQVFSKHSIEYFMDRFHRHLIETIEHCSQKKAREKTLSDFSNKELTLSA
                     LSSIEDLVKDL"
     gene            complement(9405..>13117)
                     /gene="ppsA"
                     /locus_tag="PO770_RS20895"
                     /old_locus_tag="PO770_20900"
                     /pseudo=""
     aSDomain        complement(9429..9863)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20895_TIGR01720.1"
                     /evalue="1.20E-46"
                     /label="PO770_RS20895_TIGR01720.1"
                     /locus_tag="PO770_RS20895"
                     /protein_end="1228"
                     /protein_start="1083"
                     /score="150.3"
                     /tool="antismash"
                     /translation="LAYRIKTTKDMLRRVPNKGTGYGLLTHIGELRHKEPEVSFNYLGQ
                     FSEEKEAETFQLSYYKPRHEIAGKREREYELDINALITDGRLQVKAVYTQVFSKHSIEY
                     FMDRFHRHLIETIEHCSQKKAREKTLSDFSNKELTLSALSS"
     aSModule        complement(9429..12578)
                     /complete
                     /domains="nrpspksdomains_PO770_RS20895_AMP-binding.1"
                     /domains="nrpspksdomains_PO770_RS20895_PCP.1"
                     /domains="nrpspksdomains_PO770_RS20895_Epimerization.1"
                     /domains="nrpspksdomains_PO770_RS20895_TIGR01720.1"
                     /locus_tags="PO770_RS20895"
                     /monomer_pairings="Orn -> D-Orn"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(9783..9842)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20895_0011"
                     /evalue="9.10E-09"
                     /label="NRPS-E6"
                     /locus_tag="PO770_RS20895"
                     /protein_end="1110"
                     /protein_start="1090"
                     /score="27.6"
                     /tool="antismash"
                     /translation="TKDMLRRVPNKGTGYGLLTH"
     aSDomain        complement(9888..10820)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20895_Epimerization.1"
                     /evalue="1.30E-148"
                     /label="PO770_RS20895_Epimerization.1"
                     /locus_tag="PO770_RS20895"
                     /protein_end="1075"
                     /protein_start="764"
                     /score="488.0"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVIDG"
                     /tool="antismash"
                     /translation="EGEAELTPIQRRFFGQVHAFHYHYNQSVMLFSEKGFNANALHLAL
                     RKITEHHDAIRMIFQHDQNGHVIQFNRGINHKDHELFGLYISDWTKASLERAHLDEKLA
                     AEETVIQSKMNVEKGPLLQAGLFKTAEGDHLLIALHHLVIDGVSWRILLEDLAAAYQQA
                     LEKKEIQLPPKTDSYLSYADGLTQIAESKQLLSEKPYWQTILDAHTAFLPKDIENVPDR
                     LQMNSDAAAFVLSGDWTEKLLFETQQAYGTDANELLLTALGMALSEWTGHDQIVISTEG
                     HGREGHVPNIDISRTVGWFTSIYPILLDMG"
     CDS_motif       complement(9897..9947)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20895_0010"
                     /evalue="4.40E-09"
                     /label="NRPS-E5"
                     /locus_tag="PO770_RS20895"
                     /protein_end="1072"
                     /protein_start="1055"
                     /score="28.3"
                     /tool="antismash"
                     /translation="DISRTVGWFTSIYPILL"
     CDS_motif       complement(10353..10418)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20895_0009"
                     /evalue="2.50E-11"
                     /label="NRPS-E2"
                     /locus_tag="PO770_RS20895"
                     /protein_end="920"
                     /protein_start="898"
                     /score="35.7"
                     /tool="antismash"
                     /translation="LLIALHHLVIDGVSWRILLEDL"
     CDS_motif       complement(10644..10715)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20895_0008"
                     /evalue="2.10E-02"
                     /label="C2_LCL_024-062"
                     /locus_tag="PO770_RS20895"
                     /protein_end="823"
                     /protein_start="799"
                     /score="7.3"
                     /tool="antismash"
                     /translation="FNANALHLALRKITEHHDAIRMIF"
     CDS_motif       complement(10779..10805)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20895_0007"
                     /evalue="4.40E-03"
                     /label="NRPS-E1"
                     /locus_tag="PO770_RS20895"
                     /protein_end="778"
                     /protein_start="769"
                     /score="10.3"
                     /tool="antismash"
                     /translation="LTPIQRRFF"
     aSDomain        complement(10857..11048)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20895_PCP.1"
                     /evalue="1.00E-21"
                     /label="PO770_RS20895_PCP.1"
                     /locus_tag="PO770_RS20895"
                     /protein_end="752"
                     /protein_start="688"
                     /score="68.9"
                     /tool="antismash"
                     /translation="KTMAYIWEDVLSMPRVGIHDSFFELGGDSIKALQVAARLAAEGWS
                     MTIRDLFRYSTIQELCGHI"
     CDS_motif       complement(11106..11237)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20895_0006"
                     /evalue="1.20E+00"
                     /label="C2_LCL_024-062"
                     /locus_tag="PO770_RS20895"
                     /protein_end="669"
                     /protein_start="625"
                     /score="1.6"
                     /tool="antismash"
                     /translation="SDVNTNALRAALTKELPAYMIPAHLIPLENMPLTLNGKLDRNAL"
     CDS_motif       complement(11334..11396)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20895_0005"
                     /evalue="2.40E-09"
                     /label="NRPS-A_a8"
                     /locus_tag="PO770_RS20895"
                     /protein_end="593"
                     /protein_start="572"
                     /score="29.4"
                     /tool="antismash"
                     /translation="LGRMDYQVKINGYRIETEEIE"
     aSDomain        complement(11364..12578)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PO770_RS20895_AMP-binding.1"
                     /evalue="2.80E-96"
                     /label="PO770_RS20895_AMP-binding.1"
                     /locus_tag="PO770_RS20895"
                     /protein_end="583"
                     /protein_start="178"
                     /score="314.6"
                     /specificity="substrate consensus: Orn"
                     /tool="antismash"
                     /translation="FEAKAEEIPEHIAVIDNETEISYRLLNERANRLARTLQNRKGPKP
                     TVAVLAKRSIDAIVGVLAVMKAGGVYIPIDSHYPKARIEYILRDSGADILLLQRELKHL
                     ISNSPESEMSHIFLDDEGSYEESNCNLNLSPALEEPVYIIYTSGTTGAPKGVIVTYRNF
                     THAALAWRQIYELDRKPVRLLQIASFSFDVFSGDLARTLTNGGTLIVCPDETRLEPAEI
                     YKIMNSQRITVMESTPALIIPVMEYVYRNQFKLPDLDILILGSDMVKAQDFKTLTDRFG
                     QSMRIINSYGVTEATIDSSFYETSMGGECTGDNVPIGSPLPNVHMYVLSQTDQIQPIGV
                     AGELCIGGAGVAKGYHHKPDLTQMKFTENPFVSGERLYRTGDRACWLPNGSIRLLGRMD
                     YQVKIN"
     CDS_motif       complement(11481..11570)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20895_0004"
                     /evalue="1.20E-14"
                     /label="NRPS-A_a6"
                     /locus_tag="PO770_RS20895"
                     /protein_end="544"
                     /protein_start="514"
                     /score="46.3"
                     /tool="antismash"
                     /translation="PIGVAGELCIGGAGVAKGYHHKPDLTQMKF"
     CDS_motif       complement(12102..12155)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20895_0003"
                     /evalue="1.20E-07"
                     /label="NRPS-A_a3"
                     /locus_tag="PO770_RS20895"
                     /protein_end="337"
                     /protein_start="319"
                     /score="24.0"
                     /tool="antismash"
                     /translation="YIIYTSGTTGAPKGVIVT"
     CDS_motif       complement(12354..12395)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20895_0002"
                     /evalue="7.30E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="PO770_RS20895"
                     /protein_end="253"
                     /protein_start="239"
                     /score="16.0"
                     /tool="antismash"
                     /translation="LAVMKAGGVYIPID"
     CDS_motif       complement(12918..13037)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PO770_RS20895_0001"
                     /evalue="5.70E-21"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="PO770_RS20895"
                     /protein_end="65"
                     /protein_start="25"
                     /score="66.9"
                     /tool="antismash"
                     /translation="RETALEAFEHQDYPFEELVDKLELTRDMSRNPVFDAMFIL"
ORIGIN
        1 cgctgtcttt cagcatgtag ctcagccgct cctttggata cgcaggatca agcggcaaat
       61 acgcccctcc ggccttcaat acagcaagga cagcgatcag catgtcaggt gagcgttcag
      121 acaggatacc gacgatactt tcgtttgtga cgccgcgtgc cgcaaggtgc gcagccagcc
      181 ggttggcgta catgtcaagc tcagtgtatg tcagcgaacc gccggaaaat cggatagctg
      241 gccgctccgg tgtgactgcc gcctgtcgtt caaacagccc atgcaaggtt ggggcttcag
      301 gtgaaacaga tactgtcctg ttattttgag aaacgatttt ccgtgttttg tcctcatcta
      361 gcaatgtcat ctttgacaca ggaagagatg gatcagagat gacgtcagag atcatgtgct
      421 gtaaataatc ttttatttcg gacatcatat ttggttgata agtgcctgta tgaaaatgaa
      481 tccgaacgta caaagtacgc cctgggatca ctttgacact gcatctataa ttagttacct
      541 cttccatctc aaaatctttc acggagaaat caaatgatcc agcttgattc agctcctcaa
      601 tctcttgttg agtaggtgta ttttcaaaga cgataatatg atcaatcagc tcttgtttaa
      661 gagcgctctg cgcctgaatg tcatataaag ggaagtaact atacgcttct gcttcagtca
      721 tgtccttttg cattccgctg actaggtcag aaaaagacag aaaatcactt tggactcgaa
      781 taggaatggt attgataaaa agtccgacca tgttttccac atcttttagc tcagaaggtc
      841 tgcctgatat aactgatccg aatacagcat cgtcgcgacg attgaacttt tggagcatga
      901 ttccccataa agcttggaaa acagtgttaa gtgtcgcacc acatgctgca gcgattttct
      961 gtaattcgga ggtttctttt tcaggaatgg taaacgtgat ctgctgtaat gtcccatccg
     1021 acatttgatc tgttctttta ggcagaggcg aagctttttc gaaatgctga agcctttttt
     1081 tccaatactc agctgcttct tcacgctctt gctgcatcag ccatttaata tacgtcccgt
     1141 acggctgtac gggaggcaaa tcaggaaatt gcccttttcc aagcgcttga taaatatgga
     1201 gaaagtcttt aatgacgatg cctaaacacc agccatccat taaaatatga tggtggctcc
     1261 agatacagac ataatgatca ggcgcccatt ttaaaataga tattctcatc agtacatccg
     1321 tttggagatc aaagcctttt gatttgtcct gttctttgaa tgcctctgta caatgttctt
     1381 tatctttttc attgagatga gaaatatctt caacatgaac atggcaatga cgttgcttga
     1441 gcacgacttg acgaggtttg gctacatttt tatagatgaa tgctgttcga aagatatcgt
     1501 acctgtcaaa aacagcatcc atacttttct ggaaacgctc caagtcaagc tgtccattga
     1561 tcgtaaaaga agcctgttca aaataagctc tcgactgctc atcatacaat gagtgaaaaa
     1621 gcatcccttc ctgcataaat gacaaagggt atatatcctg tatttcaggt tgctgcggca
     1681 tcagtgaatc tcctctccta tagtttgttg acggctccca tgatctcact caattcatca
     1741 agcgttaagt cttcatcagt gaaatcagat gcgctccact ctcggttttc tttgccggta
     1801 caatgctcaa tgatgttttc cagagtctgt ttaaaatgcc tggaaaatgt ttggattgtt
     1861 ttttcctcaa attgaaccct gttatagatg atgtgcatgt tcaggcaccc cgaagaaacg
     1921 gcgccgctga tatccagtgc ataaggacgc tcccagttcg ggctgatctc atttcctggc
     1981 ttgatgtcag acacctcaaa ggcatttgat tgaatatcct gatcaaactg gcctaaataa
     2041 ttaaagctga tttctgggct gtttttctga gagccccatc ttttgctgat atatttcagc
     2101 acgccgtagc ccgcgccttt atgcggtata cggtgcagca tatcttttgt ctgtttaata
     2161 aggtggccca gccgctcttc tttgtcctta tgatcggaat ctctcatatc aagccacaca
     2221 ggataaatag aggtgaacca gccaacagtt ctgctaatat cgatgtcctc aagatatgat
     2281 tcccttccgt ggccctccat gctgatttta aattggttat ttcccgtcca tttctgcaaa
     2341 gcaagaccta aagctgtcag aaggatatca ttcatctccg tgctgtaagc tttatgtgca
     2401 gccgttagta gtaattctgt gtgatggcga gatagctcaa aatcaatcac ttctgtatct
     2461 ttgaatgtgt tagatgctgc gtcattgtct tttggaatag gctggatgtg acggctctcg
     2521 cactcatccc agtacgctgc ttcgcgctgc acctggcggc ttttagagta gtcaactatt
     2581 ctttgtgaat acgttaaata tgaatctgtt ttttgcggca gtttaataga ttctcctgag
     2641 actgcctgtt tgtaagcagc tgaaaaatcc tcgaacaaaa tgcgccagga aaccgcatca
     2701 acaactaagt gatgaatcgt cagaaataaa tggtcaccat tctcggttct aaacaatcca
     2761 gcgtgaagta aggggccggt ttccaaacga atatgttctt gcatccggct ggctgcttcc
     2821 tcaatcgtgt accgtgcttc tgttaggcta tcctttacat caaacagttc aagtgcatat
     2881 agctcctcat ctgaaagatc tattcctctg tttatctgca cctgcctgcc atcttcatgc
     2941 cggcatacga ttctcagtgc atcatgatga gtcacaaggc tttttagtac ttttctaagc
     3001 gcagcctcat caaatcgttc agcccggtag atcataacgg actggttaaa atgatggctt
     3061 tccagagatt gcgagaaaaa ccactgctgg ataggtgtcc aagtaatctc gccttcgacc
     3121 gggccttggt caatggcgcg tacgtctttt cgaacccggg cggacagttc accaagagtc
     3181 tgatgttcaa acatctcgcg tactgtcgtt tcaaatccgc gctgcttcag tctggcacaa
     3241 acctgcagcg ctttaataga gtctccgcca agctcaaaga aattggcgct tacacccagc
     3301 tcatctatat taagaagctc ctcccaaatt tcagccatca ccgtttcaat ctcatttcta
     3361 ggactgatat catcagctgt ataagcgtgt ttttcaggct ctggcagggc tttctgatcg
     3421 agctttccat taacagtcac cgggatgctg tcaatttgta tcatacgggc aggcagcata
     3481 aagtcaggca aggcgttttt taggtaagtt cggatgtctg atatattcgc agcctgttga
     3541 tcttttatca ccatatatgc atatatagct gtttgccctt gctcgtctgc tctggtaatg
     3601 acagccgcct ctttaatcat tggatactga agaagtgccg cttggatttc gcctaactca
     3661 atacggtgcc ctctgacttt cacctgctca tcaatgcggc ctaagtattc cagctcgcca
     3721 tttgacaggc gcttagcaag atctcctgtt cggtataatc tgtctcccgg aagataagga
     3781 ttggatacaa aacggtcagc ggtcagctca tctctgttga gatatccccg tgccacacct
     3841 tcgccgccta tatacagctc ccctggaact ccgatcggct gcagattcat atgagcatcc
     3901 atcacataag cctgcagagt tgaaagcggc cgtccgatat tgcttttatt ttttacaatg
     3961 tctgctgcgg acaatttttt gaatgttaca tggaccgttg tttctgtaat accgtacata
     4021 ttaatcaaat cagtatgagg atatttttta ttccagcttt gaagcatgcc gggctgtaag
     4081 gcttcgccgc caaaaatcac gtagcggatg tttaaatgat ctgtatgatt ttgatcttca
     4141 agcatcaagc cgtaaaaagc agtcggggtc tgattgagta cagtgacgcg ctcttttttt
     4201 aataataaac ggaatgcatg ggggtcacgg gctgtttctt tagagaccac gactaatgta
     4261 cctccgttca ataacgcacc gaacatctcc catacagaga aatcaaagca ataagaatga
     4321 aataacgtcc agatgtcttc atggttaaat tcaaaaggca ggttttgatg cttcaagaga
     4381 ctgatcacat tgcggtgctc gacaatgact ccctttggct ggccagtcgt tccagatgtg
     4441 tatatcacgt atgcgggctg atctgttact atttcttgaa tgtttacatc agccggaaat
     4501 gagtcgtttt cacattcatc gatatacaaa atatgcccgg tgtagtctgc aggtttgttt
     4561 tgaccgcgct ctgtcagcag cagttttgct ccgctgtcat taagcataaa gctaatacgc
     4621 tccttgggat attcaggatc aatcggcaaa taggccccgc cagcttttaa aatcgataaa
     4681 acgccaatga tcatatcagg tgaccgatcg agcatcagac cgacgatatc gccagttctc
     4741 accccttgtt tgatcagcat agcagctgcc cgctcggctt tttcattcag gtctcgatac
     4801 gtaagctgag aatggccgca caccactgcg atatgctccg gatatttagc ggcttgcttt
     4861 tcaaataagc ttactatgtt ttcatttctc ggataagccg catactgtcc cattttttca
     4921 aggagcaacc tacgctctgc agcaggaagc aattgcatgt ccgaaattct catctcggta
     4981 tccgccgtta cccctttcac caattcaata aaataggatg cccactgcgt aatggtttct
     5041 tcttcaaaaa gagcagtgct gtattcaaaa cggcagcgta ttccactcgg atgctcctca
     5101 gcgtgtaatg ttaaatcgaa tttagatata tcatgtttag gagtgacaga cgaaatcgac
     5161 agaccaggca cttcgaaccg gaaatcttca ctgctttgaa gcacaagcat cgcatcgaac
     5221 agcgggttgc gatttacctc tcgatttgta aagagcttat ctgccagctc ctcaaaagga
     5281 taatcctgat gctcgtaagc tgtcagagcc aggtggcgaa tgtcctgcaa ataggagctg
     5341 aacgttttgt ggccctcggg cttgctgcgc atcgccaatg tattgacgaa catcccgatg
     5401 acgcgctcca gatcagcgtg cggtcttccg gcagcaggcg aaccgacaac aatatcctct
     5461 tgcccgctta gcttagataa caaaatagaa tagacagcca gaagaatcat atacaatgtt
     5521 gttcccgttt cagccattaa acggtgcagc tcatcagccg tgtctttatc aatgttgagt
     5581 tcaattgttc caccgctgaa atcagggaga cgcggccttg ttttatcaag cggtaattct
     5641 agtgtaggaa gactcccgcc aagctgaccg agccagtagg cctcctgttt tttgtatagc
     5701 tctttcgggt gcttctcttg ctgccaaaca gcaaaatcct tataatgcag gtttaaagct
     5761 ggcaactctt ttccgcaata taagtcagta aattcttgga caagagtgct catcgagaca
     5821 ccgtcggcaa taatatggtg catatcgagc agcaaaaagc cgcgattatt gcccattgta
     5881 atgacacatg ctctaaaaag cggaccggat gatagatcaa acggtttgat aaaggtctcg
     5941 attgcagact caagcatttg ttctgattcc atttcaagct cttttagatc aaaagctatc
     6001 tctggataaa tgatctgcac aggctcgtct ctgtcaagca caaaaacagt ccgtaatgct
     6061 tcatgccgat tcacaagttc tgtgagaacg cttctcaacc gctggcgatt cagatttccg
     6121 cgtaattcaa gtacagctgg catgttgtag cctgtgctgt catctgccag ctgatgaaga
     6181 gcatataatc gtttttgcga gaaagacagc ggatagacat ctctttgatc cgcaggttca
     6241 atctgtgtaa aagcgtgttc ttttaagctc atcaatttga ccgccatgct ttcaaccgtc
     6301 ggatatgaaa acacctcact gagcggcagc tcgacattaa gctttttatg tattcttgat
     6361 accagtgcgg ccgcttttaa ggactgtccg cctaactgaa agaaatcatc acgaatgccg
     6421 atttgctgtt tattcagcac atcttcccaa atatgtgcga gatcagcttc gattaaggtt
     6481 cggggcgcca tatatgtgac actcgtattg ttttctggtt caggaagcgc aaacctgtca
     6541 agcttgccgc tggatgttaa tggcatccgg ccgatgttga taatacttgc tgggatcata
     6601 taggacggca atgtcccggc aagatgctga tacaaatctg ggaggctgtt tcccttttct
     6661 ggaacgatat aagcaacaag ctccttttcc tcagcagaaa catcacgggc cagtaccgcg
     6721 gcttctttta ccccgggtgc ttgtcgaaga actgtttcaa tctctctcag ttcaatacgg
     6781 taacccctaa tttttacttg atcatcaaaa cgtccaatat attcaatcgt tccatcaggc
     6841 aaaaagcggc ctgcatcacc tgttcggtat aaacgctccc cttcaaggaa tggatgatcg
     6901 gagaatgctt tctgtgtcag ctctggctgt ttgtagtatc ctctcgcaag acccgcgccg
     6961 gaaatacaca gttcacccgc cgcgccgatc ggctgcattt gatttccgtg caaaacaaat
     7021 acttttgtgt ttctgatcgg gtgtccgatc gtgatcctct ctttttcatt cagatgccgc
     7081 aatattgttg ttgccacact gttttctgtg ggcccgtatt catttgccag ttcagcagaa
     7141 ggacaaatta tttgactcaa ctcaagcaga tcaggcgtga ccgcttcacc cgcaaatgtc
     7201 acaatacgaa gactttttgc atcgtcagca gtcatcactt ccaataacac cctgtacagg
     7261 gaaggaacaa tgatcatgtg gctgacctta taacgggcta tttttcgctt aagggccagc
     7321 acatccttcg cttcgtcatc tgttgtcagc agtacgcacg caccggataa aagtggggta
     7381 aataagctcg tcacacagcc gtcaaacacg tacgaaaaca aatggaggga tatatcctgt
     7441 tcggccatgc catactcttc ccgtctccat tgcaatgtat tggcaatacc gtgatgatca
     7501 actaaaaccc ctttagggcg ccccgtcgtt cccgacgtat agatcaagta ggctagatca
     7561 cctggcggca tatttggagc ttctgtttgg taactttctg tatgttctat cgttatcgta
     7621 tttccttcat aggacggagg gatcaaggaa ataaggtctt cttctatcag caattgagcc
     7681 gcttgactgt ccttcagcag gaaagaaaga cgttctgctg gaaaatgtgg atcaagcggc
     7741 atataagcgc cgcctgcttt ccaaatccca agcagagctg caggcatttc cattgaccgg
     7801 cccatcatta cagcaacgat atctccagat ccaacgcctt tttctatcag agcgtgcgca
     7861 atctgattgg ctcttacttg taactggcga taggtccagc attcgtttcc cattttcaat
     7921 gcagcagctt caggtgtttt gtgagcttgt tcctcgaaca attgaggtat tgttttgttc
     7981 acataaggac gctcagtttc gttaaacgat tggataacct gttgctcctc cggcggcacg
     8041 atattgatgt cactgacggc aacatcggga tttgtcaaaa tgcaatccag tgcttgcttt
     8101 agatgcccct gaattctcag catctcctcc cgctcgtaaa cgtctgcgtt gtagctgaat
     8161 ttgatataaa atgattctcc cggaacgata acgatattaa agtcgtaatt ggtttgttcc
     8221 gacattgaaa tgtcactcag cttcagagcg ccttcgtcat tttcactctc cacgcttaat
     8281 gcctgatgaa gctgtacagg gtaattttca aaaacaaaaa tatgatcgat caacccttgc
     8341 ttcaatggac tatggttttg aatctcataa agcggataat agccatgctg ttcagatgat
     8401 aaaatatcct cctgtacggc tttgaccagc gaagaaaacg ttatgccttc cgcagatcgg
     8461 attcgcactg gaacagtatt gataaacaag ccgaccattg attcaatccc gtcaatcgct
     8521 gacgggcgtc ccgaaatgac ggagccaaaa acggcatcat ctgcagcatt atatcgatgg
     8581 agcagcacgc cccaaatact cataaacagc gtgtttaatg tcactcccca gttttgggca
     8641 gcctctgtca gtttctcaac catatccgac gctaatgaaa agctgacctg gtcggcttta
     8701 taattcccgt ctgccgtgcg gcgctttatt cggggaatag aagcagtatt gccgtactcc
     8761 ttcaaatatt cgctccagta ggcagcagtc tcttcttgat cttgttctcc gagccattca
     8821 atataacggc tgtacggcac cgtgctgcca agctgtacag gtgagttatt tttgagcgaa
     8881 tcatacatgc tgaaaaactc tttaagaata atgccgagac accacccatc catgatgata
     8941 tggtgatgtg tccagacaca tgtatattgg ctgtctcctc tatcaaaaag ggcaagacgc
     9001 attaaaggat ctttttgcaa atcaaatcct ttttcccgat ccttagtcat aaaagcatca
     9061 atcagcgtct gctgttcttg atcagccagg cgagaaatat cttctctata tactgtcaat
     9121 tcccggtttg ataagactac ctgctgcgga cctgtaagat ctggtacttc tttaatgaaa
     9181 atcgttctga aaatatcata tctagaaaca agaaggttta agctcttttg aaacgagtcg
     9241 acgcatagat tccctgtgat cgtaaacgag gtctgttcga tatatgcttt cgagctgaaa
     9301 tccattagtg aatgaaacag cattccttcc tgcatgtgcg ataacggata aatatcttga
     9361 atttgtgccg attgtgccat caattccaca caccttttca aaagttaaag atctttcacg
     9421 agatcttcga tgctagataa tgcagacaac gttaattcct tattgctgaa atcgctcaat
     9481 gttttttcgc gtgctttttt ctgcgaacaa tgctcaatcg tctcgataag atgcctgtga
     9541 aaacgatcca taaagtactc aattgagtgt ttgctgaaca cctgggtgta taccgctttt
     9601 acttgaagcc gtccgtccgt aatgagagca ttgatgtcca gctcatactc tcgttctctc
     9661 ttacccgcta tttcgtgacg gggcttgtaa tatgataatt gaaacgtttc agcttctttc
     9721 tcttcgctaa attgaccgag ataattaaag ctgacctcag gctctttatg ccgcagttct
     9781 cctatgtgtg ttaacaatcc gtatccagtt cctttgttcg gtactcttct cagcatatct
     9841 tttgtcgtct taattcggta tgcgagctga tcctcgaaag gctcgggaat acccatgtct
     9901 agcaaaatag gataaatgga ggtaaaccat ccaacggtcc ttgaaatatc aatgtttggc
     9961 acatgccctt ctctgccgtg tccctcagtg ctgatcacga tttggtcatg ccctgtccat
    10021 tcagaaagcg ccattcctaa agccgtaagc aaaagttcgt tggcatcagt accataggct
    10081 tgctgggttt caaacagcaa tttttcagtc cagtccccgg atagtacaaa cgcagctgcg
    10141 tcgctgttca tttggagtct gtccggcaca ttttctatgt ccttaggaag aaaagcagtg
    10201 tgagcgtcta aaatcgtttg ccaatacggt ttctctgaaa gcaattgttt actttcagct
    10261 atttgggtaa gaccatcggc gtaggataaa taagaatccg tttttggcgg taattggatt
    10321 tctttctttt ctaaagcctg ttgatatgct gcagctaaat cttcaagcag aattctccaa
    10381 gaaacgccat ctatcacaag atgatgaagt gcaatcagca aatgatcccc ttctgccgtt
    10441 ttaaacaagc ctgcttgcag caaaggccct ttttctacat tcattttgct ttgaatgacg
    10501 gtttcctcag cggcaagttt ttcatcaaga tgagcccttt ccagcgatgc ttttgtccaa
    10561 tctgaaatat aaaggccaaa cagttcatga tccttgtgat tgattccgcg attaaactga
    10621 atgacatggc cgttttggtc atgttggaaa atcatgcgaa tggcatcatg atgctcggtt
    10681 attttacgca gcgctagatg aagcgcgtta gcattgaatc ctttttcact gaaaagcatg
    10741 acggactggt tgtaatgata gtgaaaagca tgtacctgtc cgaaaaatct tcgctgaatc
    10801 ggggtgagtt cagcttcacc ttccgcaggt ccctggtccg cttgagatgc caatggtgtg
    10861 atatgtccgc atagttcctg aatcgttgaa taacggaata agtcgcgtat tgtcatgctc
    10921 cagccttcag cggccagacg tgctgccact tgtaatgctt taattgagtc tccgccaagc
    10981 tcaaaaaagg aatcgtgaat gccgactcgt ggcatgctca gtacatcttc ccatatatac
    11041 gccatcgttt tttgtatgtc gttaactggt gcggtatatg gacgcgataa cacattattt
    11101 ggagccggca aggcatttct gtccagcttt ccgtttaggg tcagcggcat gttttctaaa
    11161 ggaatcagat gtgcaggaat catatacgct ggcagctcct ttgttaaagc agcacggaga
    11221 gcattcgtat tgacgtctga aggcacaata tatgctgcca aaccagcctg tccattttta
    11281 tcatgctgaa cggcaacagc agcttctctg acaagccctg tttgaagcag aacactttca
    11341 atttcttctg tttcaattct gtatccattt atctttactt gataatccat tcggccaaga
    11401 agccggatcg acccgtttgg aagccagcat gctcgatcac cggttctgta cagcctttct
    11461 ccagagacaa atggattttc tgtaaatttc atttgcgtta agtcaggttt atgatgatag
    11521 cctttcgcaa cacctgcccc gccgatgcac agctcgccgg caacaccgat cggctgaatt
    11581 tgatcagtct ggctgagtac atacatgtgt acattcggca gcgggctgcc gattggtacg
    11641 ttatctcccg tacactctcc acccatacta gtttcataga aactcgaatc aatggttgcc
    11701 tctgtgacac catagctatt aatgatgcgc atgctttggc caaagcgatc tgtcaatgtt
    11761 ttgaaatctt gagctttcac catgtcggaa ccaagaatca gtatgtctaa gtcaggaagc
    11821 ttgaactggt tacggtatac atattccatc accgggataa tgagagccgg tgtcgactcc
    11881 atcaccgtga ttctctgaga gttcatgatt ttatatattt cagcaggttc cagccgtgtt
    11941 tcatctgggc aaacgattag agtcccgccg ttggtcaatg ttctcgccaa atctcctgag
    12001 aagacatcga atgaaaagct cgcgatttga agcagtctga caggcttccg atccagctca
    12061 taaatttgac gccaagcaag agcagcatga gtaaagttcc gataggtgac aataacgcct
    12121 ttaggcgcac ccgtcgttcc tgacgtataa atgatatata ccggctcttc aagagcagga
    12181 gataagttaa ggttgcagtt actttcttca taagagccct catcgtcaag gaagatgtga
    12241 gacatctcag attcaggcga gttcgaaatc aaatgcttca actctctctg taaaagaaga
    12301 atatctgccc cgctatcacg aagaatgtat tcaatacgag cttttggata atgtgaatcg
    12361 attgggatat acactccgcc agctttcatc actgccagaa ctccaacaat tgcatcgatg
    12421 gaccgcttgg ctaaaacagc cacagttggc ttcggtcctt ttctgttttg caatgttcgt
    12481 gccagacgat tggctctttc atttagaagg cgatagctaa tctctgtttc gttatcaata
    12541 acagctatat gttctggaat ttcttcagct ttcgcttcaa atatgtgatg aaaaggaaca
    12601 tctttttgag caaattcaac ctgagttttg ttaaatccaa agactatttt ttgtttttct
    12661 ttatcactaa gtatgtttat ttgtgcaagg cttgtttcag gagttgaaag tgccgcatgc
    12721 agaaattcga taaaatgatc ggcccagcgt tcaattgtag ctttcaggaa tacttctgta
    12781 ctgaattcca tttcacagca aatagatcca tttatttccg tagctattaa tgtgatatca
    12841 aataaggaaa tgtgatgtgc gaaattggca ggccggactt ttatttctct cagatctatg
    12901 tcttgttttt ccacattttg aaggataaac atcgcatcaa acactggatt ccggctcata
    12961 tcccgtgtca attcaagctt atcgacaagt tcttcgaacg ggtaatcctg gtgctcaaag
    13021 gcttccaatg ctgtctcacg cacttcctgc aaatactgta cgaatggctt cccgccctcc
    13081 gggcgcgtcc gaagcgccag cgtgttcaca aacatgc
//
