LOCUS       NZ_JAQOXM010000001     20747 bp    DNA     linear   CON 10-MAR-2024
DEFINITION  Bacillus subtilis strain CWTS 5 NODE_1_length_1063700_cov_36.2731,
            whole genome shotgun sequence.
ACCESSION   NZ_JAQOXM010000001
VERSION     NZ_JAQOXM010000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 1063700)
  AUTHORS   Natarajan,D.A., Chandwani,S. and Dewala,S.
  TITLE     Complete genome sequencing of Bacillus subtilis (CWTS 5), a
            siderophore-producing bacterium triggers antagonistic potential
            against Ralstonia solanacearum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1063700)
  AUTHORS   Natarajan,D.A., Chandwani,S. and Dewala,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2023) Molecular Biology Unit, Uka Tarsadia
            University, Bardoli, India, Maliba campus, Bardoli, Gujarat 394620,
            India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-JUL-2022
            Assembly Method                   :: SPAdes v. 3.11.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 02:26:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,268
            CDSs (total)                      :: 4,150
            Genes (coding)                    :: 4,020
            CDSs (with protein)               :: 4,020
            Genes (RNA)                       :: 118
            rRNAs                             :: 10, 12, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 11, 6 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 77 of 130
            Pseudo Genes (internal stop)      :: 19 of 130
            Pseudo Genes (multiple problems)  :: 23 of 130
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:37:08
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 618927
            Orig. end                         :: 639674
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAQOXM010000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20747
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="CDPS"
                     /region_number="5"
                     /rules="CDPS"
                     /tool="antismash"
     cand_cluster    1..20747
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="CDPS"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20747
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="False"
                     /core_location="[10000:10747](+)"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10747
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     gene            1631..4504
                     /gene="uvrA"
                     /locus_tag="PO770_RS03150"
                     /old_locus_tag="PO770_03155"
     CDS             1631..4504
                     /EC_number="3.1.25.-"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_process="GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="uvrA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391396.1"
                     /locus_tag="PO770_RS03150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_03155"
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="WP_003228057.1"
                     /transl_table=11
                     /translation="MAMDRIEVKGARAHNLKNIDVTIPRDQLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMDKPDVDAIEGLSPAISIDQKTTSRNPRSTVGTVT
                     EIYDYLRLLYARVGKPHCPEHGIEITSQTIEQMVDRILEYPERTKLQVLAPIVSGRKGA
                     HVKVLEQIRKQGYVRVRIDGEMAELSDDIELEKNKKHSIEVVIDRIVVKEGVAARLSDS
                     LETALRLGEGRVMIDVIGEEELMFSEHHACPHCGFSIGELEPRLFSFNSPFGACPTCDG
                     LGMKLEVDADLVIPNQDLSLKENAVAPWTPISSQYYPQLLEAVCTHYGIDMDVPVKDLP
                     KHQLDKVLYGSGDDLIYFRYENDFGQIREGEIQFEGVLRNIERRYKETGSDFIREQMEQ
                     YMSQKSCPTCKGYRLKKEALAVLIDGRHIGKITELSVADALAFFKDLTLSEKDMQIANL
                     ILREIVERLSFLDKVGLDYLTLSRAAGTLSGGEAQRIRLATQIGSRLSGVLYILDEPSI
                     GLHQRDNDRLISALKNMRDLGNTLIVVEHDEDTMMAADYLIDIGPGAGIHGGQVISAGT
                     PEEVMEDPNSLTGSYLSGKKFIPLPPERRKPDGRYIEIKGASENNLKKVNAKFPLGTFT
                     AVTGVSGSGKSTLVNEILHKALAQKLHKAKAKPGSHKEIKGLDHLDKVIDIDQAPIGRT
                     PRSNPATYTGVFDDIRDVFAQTNEAKVRGYKKGRFSFNVKGGRCEACRGDGIIKIEMHF
                     LPDVYVPCEVCHGKRYNRETLEVTYKGKSISDVLDMTVEDALSFFENIPKIKRKLQTLY
                     DVGLGYITLGQPATTLSGGEAQRVKLASELHKRSTGRTLYILDEPTTGLHVDDIARLLV
                     VLQRLVDNGDTVLVIEHNLDIIKTADYIVDLGPEGGAGGGTIVASGTPEEITEVEESYT
                     GRYLKPVIERDKTRMKSLLKAKETATS"
     gene            <4585..5148
                     /locus_tag="PO770_RS03155"
                     /old_locus_tag="PO770_03160"
                     /pseudo=""
     CDS             <4585..5148
                     /GO_function="GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /GO_process="GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391416.1"
                     /locus_tag="PO770_RS03155"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_03160"
                     /product="flagellin"
                     /pseudo=""
                     /transl_table=11
                     /translation="RFRSELTVQAGNTGTQQAEDLGAIKDEMDALIEEVDGISNRTEFN
                     GKKLLDGTETDGFTFQIGANAGQQLTVNIDSMSSTALGVNALNVTDFANTPFDTQLESI
                     DTAINNVSNQRAKLGAVQNRLEHTINNLGASSENLTAAESRIRDVDMAKEMSDFTKNNI
                     LSQASQAMLAQANQQPQNVLQLLR"
     gene            complement(5347..5577)
                     /locus_tag="PO770_RS03160"
                     /old_locus_tag="PO770_03165"
     CDS             complement(5347..5577)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391394.1"
                     /locus_tag="PO770_RS03160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_03165"
                     /product="hypothetical protein"
                     /protein_id="WP_003235419.1"
                     /transl_table=11
                     /translation="MEKDPSDYTVTQESVLKLIHEQKRMNREMIAELEQIHGPFPISHD
                     IQYIKVLLDSSNTHIVQDLMSVSKQLYKKTL"
     misc_feature    5546..5561
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.68"
                     /tool="antismash"
     gene            5795..6121
                     /gene="yvlA"
                     /locus_tag="PO770_RS03165"
                     /old_locus_tag="PO770_03170"
     CDS             5795..6121
                     /codon_start=1
                     /gene="yvlA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391393.1"
                     /locus_tag="PO770_RS03165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_03170"
                     /product="protein YvlA"
                     /protein_id="WP_032726939.1"
                     /transl_table=11
                     /translation="MNRNQAIIASLCYFSVFIAPIIVPIVAYFVVNEKETKRHAIRSLI
                     SHIVPFVGWLFLFIALLGGAVAIDGDSLLPVFVIIGGAVIYFLVVIGIIIWNVIQGIKV
                     LREA"
     gene            6146..7243
                     /gene="yvlB"
                     /locus_tag="PO770_RS03170"
                     /old_locus_tag="PO770_03175"
     CDS             6146..7243
                     /codon_start=1
                     /gene="yvlB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391392.1"
                     /locus_tag="PO770_RS03170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_03175"
                     /product="DUF4097 domain-containing protein"
                     /protein_id="WP_014478112.1"
                     /transl_table=11
                     /translation="MKQEKERILKLVEEGKLTAQEALTLIEKLDSDYKEKEEKITALSV
                     HVHDEEEPFTTAKKESGKPSLGAKLFDWIDSAVKKVKEVDLDLNFGHAYDVQHIFQFKD
                     TDFSSVELQIANGSVNIVPWEDDDIRAECQAKVYRADSQDAARHAFLQHIECEIKGNKF
                     FIRTEKKTMKTNVTLYIPQKEYDKIRVKLFNGPVRGEHLHVKEFSAKTTNGVLSFSHLT
                     AEKAIAETANGQIKLASHSCGTIEAETINGLIDLRGKSESIDVQSFNGNIAINVTESDC
                     RSIYAKTTTGSVELAIPDDLAVKAELKSNLGSLSHELMDVEMLKEKNDTIQKEMMFTSN
                     QAHDQNITVFSESLTGAIKLKYSQR"
     gene            7248..7445
                     /gene="yvlC"
                     /locus_tag="PO770_RS03175"
                     /old_locus_tag="PO770_03180"
     CDS             7248..7445
                     /codon_start=1
                     /gene="yvlC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391391.1"
                     /locus_tag="PO770_RS03175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_03180"
                     /product="PspC domain-containing protein"
                     /protein_id="WP_003228065.1"
                     /transl_table=11
                     /translation="MNKLYRSEKNKKIAGVIGGLAEYFNWDASLLRVITVILAIMTSVL
                     PVLLIYIIWIFIVPSERDMK"
     gene            7447..7806
                     /gene="yvlD"
                     /locus_tag="PO770_RS03180"
                     /old_locus_tag="PO770_03185"
     CDS             7447..7806
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene="yvlD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391390.1"
                     /locus_tag="PO770_RS03180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_03185"
                     /product="phage holin family protein"
                     /protein_id="WP_003228066.1"
                     /transl_table=11
                     /translation="MVKWAVSILVNALLLIVIDGYIDSIHISSIGAAIIASLILSILNV
                     LIKPLLIIFTLPVTMVTLGLFLFVINAITLMMTASIMGDSFQIDGFGTAIWASVILSVF
                     HLLIQKGILEPLRKK"
     gene            complement(7834..9045)
                     /gene="pchE"
                     /locus_tag="PO770_RS03185"
                     /old_locus_tag="PO770_03190"
     CDS             complement(7834..9045)
                     /codon_start=1
                     /gene="pchE"
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391389.1"
                     /locus_tag="PO770_RS03185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_03190"
                     /product="MFS transporter"
                     /protein_id="WP_003228068.1"
                     /transl_table=11
                     /translation="MAHTKSKAVLILYTVCFSAFFASLSQNIYSPILPIIKESFHVSTA
                     MVNLSVSVFMIVTAIMQIILGAIIDFKGARIVLITGILATAAASIGCAVTTDFTLFLIF
                     RMIQAAGSAALPLIAATTIGQLFTGNERGSAMGTYQMLLSVAPAIAPVLGGFIGGAAGY
                     EGIFWILAAISIVLLVTNSITFPKDSPTESMQQAKGNVFAHYKSIFTNRTGNVILTLSF
                     VLFFIYFAVIVYLPILLTEHYHIDVGIAGLLYLPLALSTIAGTFLFKRIQAKIGLHTLF
                     IGSNVIAACSIILFAVTHSVSLVLMALTLALFGISMGVIPPLYSTMITNEFEHNRGSAI
                     GMFNFIRYTGMAAGPMVSAYLLTMMPSAMSFSLLGLGFAALSFCLLPPMFSPQKRTKQK
                     KHHM"
     gene            complement(9066..9575)
                     /gene="pchR"
                     /locus_tag="PO770_RS03190"
                     /old_locus_tag="PO770_03195"
     CDS             complement(9066..9575)
                     /codon_start=1
                     /gene="pchR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391388.1"
                     /locus_tag="PO770_RS03190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_03195"
                     /product="pulcherriminic acid biosynthesis transcriptional
                     regulator PchR"
                     /protein_id="WP_041850480.1"
                     /transl_table=11
                     /translation="MSDLTKQMIYDIYVRLLHLNEQKANTLLQQFFKEAAEEDVAEIPK
                     NMTSIHVIDCIGQHEPINNAGIARKMNLSKANVTKISTKLIKEEFINSYQLTDNKKEVY
                     FKLTRKGRRIFDLHEKLHKKKELAFYQFLDSFSQEEQKAVLKFLEQLTSTLEAEQTDGT
                     PDKPVK"
     misc_feature    9857..9872
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 22.77"
                     /tool="antismash"
     misc_feature    complement(9859..9873)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.98"
                     /tool="antismash"
     misc_feature    complement(9860..9875)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 21.5"
                     /tool="antismash"
     gene            10001..10747
                     /gene="pchC"
                     /locus_tag="PO770_RS03195"
                     /old_locus_tag="PO770_03200"
     CDS             10001..10747
                     /EC_number="2.3.2.22"
                     /codon_start=1
                     /gene="pchC"
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391387.1"
                     /locus_tag="PO770_RS03195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_03200"
                     /product="cyclo(L-leucyl-L-leucyl) synthase"
                     /protein_id="WP_021480699.1"
                     /sec_met_domain="CDPS (E-value: 4.9e-84, bitscore: 277.4,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGMVTERRSVHFIAEPLTENCREIFERRRHVLVGISPFNSRFSE
                     DYIYRLIGWAKAQFKSVSVLLAGHEAANLLEALGTPRGKAERKVRKEVSRNRRFAERAL
                     VAHGGDPKAIHTFSDFMDNKAYQLLRQEVEHAFFEQPHFRHACLDMSREAIIGRARGVS
                     LMMEEVSEDMLNLAVEYVIAELPFFIGAPDILEVEETLLAYHRPWKLGEKISNHEFSIC
                     MRPNQGYLIVQEMAQMLSEKRITCEG"
     gene            10763..11979
                     /gene="cypX"
                     /locus_tag="PO770_RS03200"
                     /old_locus_tag="PO770_03205"
                     /pseudo=""
     CDS             10763..11979
                     /codon_start=1
                     /gene="cypX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391386.1"
                     /locus_tag="PO770_RS03200"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_03205"
                     /product="pulcherriminic acid synthase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSQSIKLFSVLSDQFQNNPYAYFSQLREEDPVHYEESIDSYFISR
                     YHDVRYILQHPDIFTTKSLVERAEPVMRGLYWPKCMEKNTLPKEEL"
     gene            complement(12018..12479)
                     /gene="yvnA"
                     /locus_tag="PO770_RS03205"
                     /old_locus_tag="PO770_03210"
     CDS             complement(12018..12479)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene="yvnA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391385.1"
                     /locus_tag="PO770_RS03205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_03210"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_021480697.1"
                     /transl_table=11
                     /translation="MNNSIHDELFQAIQQFALKRDKRVWQKVQIPSSQHLDHLKKDWTL
                     TQLHIVSCLHTSKNVNNSFLASRLHISKAAVSKAIQALLKHNIITVTKKPGNKKEIFYT
                     LTESGKELAGLHERLHQKAKEQYKQLFNEFSIDELKTVTAFINLWIKHM"
     gene            12644..16513
                     /gene="yvnB"
                     /locus_tag="PO770_RS03210"
                     /old_locus_tag="PO770_03215"
     CDS             12644..16513
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="yvnB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391384.1"
                     /locus_tag="PO770_RS03210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_03215"
                     /product="metallophosphoesterase"
                     /protein_id="WP_077671021.1"
                     /transl_table=11
                     /translation="MRKYTVIASILLSFLSVLSGGHHESKAFPVVQQELFQTPHYIPLL
                     ENPPQIDVKAEMRNNLMIEAQVRDNSYQAFSAFLFYKQSNELGYKMVPMDPTPGAVRKF
                     LAQIPKRLIWNSELEYYIVMSNGKQRVESETKKLKLEGYQADLAHIPELLITELAVDTK
                     NIGRADGYEFIEIFNTTDRTIDFKDYHFRYRYPKEGPDSDLIWRPDERILIPSGETFVV
                     WLKPAGHPELTSEDFNRYYQTQLKEGKNLAVIDETEGMANTRPRAVVISTNTGKDISVA
                     HYRKHALRRLSSVLYKYPLNGTAELLNISIGEKNPSPGTVLQAQVPEQKRKIKLDKEKP
                     VIEDLTDRKPVRPAESIELRADIRDRSLVKTAAFYYRTDENKPFKRILAEKDRNDNLYH
                     YIVYSPELIGKDQLEYYVAAGDGINEARTPVKMIDIKQTSKSHGLRMNIENRDTLSGTQ
                     FLKATTEGRADRIKLWIDGKKQVTEPAMEKEVYFAFDTRKTNLYFKNAVTMEGKVLKVF
                     DDTTNKYRTYSVPLPETLLRKGKQLQRITIRSGSKVSPFDTAENRDDFLVKNARLVLSD
                     GTVIRDQRVSPEKELFIGDNQRSNKSWQFQFNLPDGLFTSQLLEWDTSKLSEGAHHIQA
                     SDGKENVSLVVRVDNSGPHIEPNITEGQTYKGNLILQADMYDKWSKIEEAEASLDGESI
                     TLPYHTSSSDLLPGKHSLKVTATDLAGNKAVIERTFKTEREHPDQPEVIDSDADTHKAK
                     LSVRVKDPTNDAMDVGFYRGFQYTARNHVKIFKHASLTEPPKSFVPERETPFTNKELER
                     VSAADGKTVSTENKELFPYHRFEVTVDPSIDENDLAEAVWKGSSLPGRKVTMYAWNYRT
                     NEWQPVDSFVAKDDKPFTLKASVIAADFVRESKMNVIVQDEIPPAKDMYTFVWMSDTQY
                     YAESYPHIFDKQTEWIKDNQKQLNIKYVFHTGDIVDDSADIRQWKNADRSMSVLDKNGI
                     PYGVLAGNHDVGHKDGSYRAFGKYFGSDRFDKKLHYGGSYKNNRGHYDLISSNGNDYIM
                     LYMGWGITDEDIAWMNQVLKKHPDRMAILAFHEYLLVSGNRSPIGEKIFKEIVKPNPNV
                     VMVLSGHYHSAMRKTDELDDDGDGKPDRLVHQMLADYQGGPEGGQGYLRLLQFDQANDM
                     VHVSTYSPYVKDKNYYDTDTYGNKDEFSLSLDLKPRIKKVETDYFECNVYTNEELGKRE
                     QVKSGDTAEFRWDHLEPQSVYYWYIIVEDSFNGKTKSPIWKFKTKKETYRPAPDQFDFR
                     HVSNP"
     gene            complement(16532..17263)
                     /gene="nagR"
                     /gene_synonym="yvoA"
                     /locus_tag="PO770_RS03215"
                     /old_locus_tag="PO770_03220"
     CDS             complement(16532..17263)
                     /codon_start=1
                     /gene="nagR"
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /gene_synonym="yvoA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391383.1"
                     /locus_tag="PO770_RS03215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_03220"
                     /product="N-acetylglucosamine utilization transcriptional
                     regulator NagR"
                     /protein_id="WP_003228089.1"
                     /transl_table=11
                     /translation="MNINKQSPIPIYYQIMEQLKTQIKNGELQPDMPLPSEREYAEQFG
                     ISRMTVRQALSNLVNEGLLYRLKGRGTFVSKPKMEQALQGLTSFTEDMKSRGMTPGSRL
                     IDYQLIDSTEELAAILGCGHPSSIHKITRVRLANDIPMAIESSHIPFELAGELNESHFQ
                     SSIYDHIERYNSIPISRAKQELEPSAATTEEANILGIQKGAPVLLIKRTTYLQNGTAFE
                     HAKSVYRGDRYTFVHYMDRLS"
     gene            complement(17281..18009)
                     /gene="nagB"
                     /locus_tag="PO770_RS03220"
                     /old_locus_tag="PO770_03225"
     CDS             complement(17281..18009)
                     /EC_number="3.5.99.6"
                     /GO_function="GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA]"
                     /GO_process="GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="nagB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391382.1"
                     /locus_tag="PO770_RS03220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_03225"
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="WP_038829909.1"
                     /transl_table=11
                     /translation="MKVMECQTYEELSQKAARITADTIKEKPDAVLGLATGGTPEGTYR
                     ELIRLHQTENLSFQNVTTVNLDEYAGLSSDDPNSYHFYMNDRFFQHIDSKPSRHFIPNG
                     NADDLEAECRRYEQLVESLGGTDIQLLGIGRNGHIGFNEPGTSFKSRTHVVTLNEQTRQ
                     ANARYFPSIDSVPKKALTMGIQTILSSKRILLLISGKSKAEAVRKLLEGNISEDFPASA
                     LHLHSDVTVLIDREAASLRP"
     gene            complement(18006..19196)
                     /gene="nagA"
                     /locus_tag="PO770_RS03225"
                     /old_locus_tag="PO770_03230"
     CDS             complement(18006..19196)
                     /EC_number="3.5.1.25"
                     /GO_function="GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA]"
                     /GO_process="GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="nagA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391381.1"
                     /locus_tag="PO770_RS03225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_03230"
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="WP_046160894.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.1e-42, bitscore:
                     142.4, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAESLLIKDIAIVTENEVIKNGYVGINDGKISTVSTERPKESYSK
                     EIQAPADSVLLPGMIDIHIHGGYGADTMDASFSTLDIMSSRLPEEGTTSFLATTITQEH
                     GNISQALVNAREWKAAEESSLLGAELLGIHLEGPFVSPKRAGAQPKEWIRPSDVALFKK
                     WQQEAGGLIKIVTLAPEEDQHFELIRHLKEESIIASMGHTDADSAMLSDAAKAGASHMT
                     HLYNAMSPFHHREPGVIGTALAHDGFVTELIADGIHSHPLAAKLAFLAKGSSKLILITD
                     SMRAKGLKDGVYEFGGQSVTVRGRTALLSDGTLAGSILKMNEGARHMREFTNCSWTDIA
                     NITSANAAKQLGIFDRKGSVTVGKDADLVIVSSDCEVILTICRGNIAFISKEADQI"
     misc_feature    complement(19251..19266)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.26"
                     /tool="antismash"
     gene            19378..20310
                     /gene="hprK"
                     /locus_tag="PO770_RS03230"
                     /old_locus_tag="PO770_03235"
     CDS             19378..20310
                     /GO_function="GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /gene="hprK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391380.1"
                     /locus_tag="PO770_RS03230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_03235"
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="WP_003228097.1"
                     /transl_table=11
                     /translation="MAKVRTKDVMEQFNLELISGEEGINRPITMSDLSRPGIEIAGYFT
                     YYPRERVQLLGKTELSFFEQLPEEEKKQRMDSLCTDVTPAIILSRDMPIPQELIDASEK
                     NGVPVLRSPLKTTRLSSRLTNFLESRLAPTTAIHGVLVDIYGVGVLITGKSGVGKSETA
                     LELVKRGHRLVADDCVEIRQEDQDTLVGNAPELIEHLLEIRGLGIINVMTLFGAGAVRS
                     NKRITIVMNLELWEQGKQYDRLGLEEETMKIIDTEITKLTIPVRPGRNLAVIIEVAAMN
                     FRLKRMGLNAAEQFTNKLADVIEDGEQEE"
ORIGIN
        1 ctgagacact ccgccacatc agctttgttt gagcgcagag atgtcattat tattgcgagt
       61 gtgtcttgta tatacggcct cggttcgcct gaggaatacc gggaaatggt cgtgtcactg
      121 cggactgaaa tggaaattga gcgcaatgag ctgctcagaa aacttgtaga catccaatat
      181 gcccgcaacg atatcgactt tcagcgcggt acatttcgtg tgcgcgggga tgtagtggaa
      241 atctttccgg catcccgtga tgagcattgt atcagagttg aatttttcgg tgacgaaatc
      301 gaacggattc gggaagtgga tgcgctgaca ggagaaattc tcggcgaccg agatcatgtt
      361 gcgattttcc cggcatccca cttcgtaacg cgggccgaga aaatggagaa ggcgattcaa
      421 aatatcgaga aggagcttga ggagcagctg aaggtcatgc acgaaaacgg aaagctcctt
      481 gaagcgcagc gtttagagca gcggacaaga tatgatcttg aaatgatgcg tgaaatgggt
      541 ttctgctccg gcatcgagaa ctattcaaga catttgactc tgcggcctcc aggttcaacg
      601 ccgtatacgc ttcttgatta tttcccagac gattttatga tcgtggtgga tgagtcgcat
      661 gtgacaattc cccaggtgcg cggcatgttt aacggagacc aggcgcggaa acaggtgctc
      721 gtggatcacg gcttccggct tccatcagcg cttgataacc gtccgcttcg ttttgaggaa
      781 tttgaaaagc atatgcacaa tatcgtgtat gtatcagcaa caccagggcc gtacgagatt
      841 gaacatacag atgaaatggt cgaacagatc attcgtccga cggggcttct tgacccgctt
      901 attgacgtcc gtccaatcga aggtcagatt gacgacttga tcggcgaaat tcaagcaaga
      961 atcgagcgaa atgaacgggt gcttgtgaca actttgacga agaagatgtc agaggatctg
     1021 accgattatt taaaggaaat cggcattaaa gtaaactatc tgcattctga aatcaagacg
     1081 cttgaacgga ttgaaattat ccgcgacctg cgccttggca agtacgatgt gcttgtcggc
     1141 atcaacctgc tgagggaagg tttggacatt cccgaagtat cccttgttgc gattttggat
     1201 gcggataagg aaggtttcct tcgttcggag cggtcgctga tccagaccat cggacgggca
     1261 gcgagaaatg cggaaggccg cgtcattatg tacgccgata aaataacgaa gtctatggaa
     1321 attgcgatta atgaaacaaa acgccgccgc gagcagcagg agcggtttaa tgaagagcac
     1381 ggcattacgc cgaaaacgat aaataaagaa attcgcgatg tcattcgtgc cacagtagca
     1441 gctgaggata aagcggaata caaaacaaaa gccgcgccga agctgtcgaa aatgacgaag
     1501 aaagaacgcc agaaagtcgt tgaacagatg gagcatgaaa tgaaggaagc cgccaaggcg
     1561 cttgactttg agagagccgc ggagcttcgc gatttacttt tagagctaaa agcggaagga
     1621 tgatgaacaa atggctatgg atcggataga ggtgaaggga gccagggcgc ataacctgaa
     1681 aaatatagat gtaacgattc cgagagatca gcttgtcgtt gtcacgggtt tgtccggatc
     1741 aggtaaatcc tcccttgcct ttgacacgat atatgctgaa ggacagagac ggtatgtcga
     1801 gtcgctgtct gcttatgccc gccagttttt agggcaaatg gataagcctg atgtggatgc
     1861 aattgagggg ctctctcccg ccatcagcat tgatcagaaa acaacaagcc gtaatccgag
     1921 gtctactgtc ggtacggtaa ctgagattta tgattatctg cgtcttttat atgcgagagt
     1981 agggaagcct cattgtccgg agcacggaat tgagattaca tcccagacca tcgagcaaat
     2041 ggtggacaga attctggaat acccggaacg gacgaagctt caggtgctgg cgccgattgt
     2101 ctcgggccga aaaggcgctc atgtcaaagt gcttgaacag attagaaaac aaggctatgt
     2161 cagagtcaga attgacggcg agatggctga gctttccgac gatatcgaat tagaaaagaa
     2221 caagaagcat tccattgagg tagtcattga ccggattgtc gtgaaagaag gcgtggcagc
     2281 ccggctgtct gattcattgg aaacggcgct ccgtttaggt gaaggacggg ttatgatcga
     2341 tgtcatcggt gaggaagagc tgatgttcag cgagcatcat gcctgtccgc actgcggatt
     2401 ttcaattggt gagcttgagc cgcgtctgtt ttcgtttaac agtccgttcg gggcgtgtcc
     2461 gacgtgtgac ggtctcggaa tgaagcttga agtggatgcc gatcttgtca ttcccaatca
     2521 agatttgtca ttgaaggaga atgcggtcgc cccttggaca ccgatcagct cacaatatta
     2581 tcctcagctg cttgaggcag tctgcaccca ctacgggatt gatatggatg tgccggtcaa
     2641 agatttgccg aagcatcaac tggataaagt gctgtacggc agcggagatg acctgattta
     2701 tttccgatat gaaaatgatt ttggacaaat ccgcgaaggt gaaattcaat ttgaaggcgt
     2761 attgcgcaac attgaaagac gctataagga gacaggctcc gatttcatac gtgagcaaat
     2821 ggagcagtat atgtctcaga agtcttgtcc gacgtgcaaa ggctatcggt taaagaaaga
     2881 ggcgcttgcc gtactgattg acggccgcca cattggaaaa attaccgagc tgtctgtcgc
     2941 cgacgcactt gccttcttta aagaccttac cctttctgag aaggatatgc agatcgccaa
     3001 tttgattttg cgcgaaattg tggagcgctt aagctttctg gacaaagtcg gcctcgatta
     3061 cctgacattg agcagggcag cgggtacatt gtccggggga gaggcacagc gcatcaggct
     3121 ggcgactcaa attggctcgc gtttatccgg tgtgctttat attttagatg agccgtctat
     3181 cggtctgcat cagcgtgata acgaccgctt gatcagcgct ctgaaaaata tgagagacct
     3241 cgggaacacg ctgattgttg tcgaacatga tgaggacacg atgatggcag cagattattt
     3301 aatagatatt ggaccgggag ccggcattca cggcggacag gtgatatctg cgggtacgcc
     3361 ggaagaagtg atggaagatc caaactcatt aacgggcagc tatttatcag ggaaaaagtt
     3421 tatcccattg cctcctgaaa gaagaaagcc ggacggacgt tacattgaaa ttaaaggtgc
     3481 ttcagaaaac aacctgaaaa aagtgaatgc caagttcccg cttgggacgt ttacagcagt
     3541 tacaggtgtt tccggttcag gaaagagtac actcgttaat gaaattttgc ataaggcgct
     3601 ggcgcaaaag cttcataaag cgaaagcgaa gcccggcagc cataaagaga ttaaaggttt
     3661 ggatcattta gacaaagtca ttgacattga ccaggcgcca attggaagaa cgccgagatc
     3721 caaccctgcg acatacaccg gtgtatttga tgacattcgt gatgtattcg cgcagacaaa
     3781 tgaagcgaag gtccgcggct ataaaaaagg ccgtttcagc ttcaacgtga agggcggacg
     3841 atgtgaagcc tgccgcggag acgggattat taaaattgaa atgcacttcc ttcctgacgt
     3901 atacgttcca tgcgaggtgt gtcacggcaa acgctataac cgtgaaacgc ttgaagtgac
     3961 gtacaaagga aaaagcatct ctgatgtgct tgatatgacg gttgaagatg ctctttcttt
     4021 ctttgaaaat atcccgaaaa tcaaacgcaa gctccaaacc ctttatgatg ttggtttagg
     4081 ttatattacg ctcggccagc cggcgacgac cttgtcaggc ggagaagcgc agcgcgtgaa
     4141 gctcgcgtca gagctgcaca aacgctcgac cggacgcacg ctctacattt tagatgagcc
     4201 aacgacaggt ttgcatgtcg acgatatcgc caggcttctt gtcgtgctgc aacggttggt
     4261 agacaacgga gacactgtac ttgttattga gcacaacctt gatatcatta agacagccga
     4321 ttacattgtg gatttgggcc cggaaggcgg agccgggggc ggaaccattg tcgcgtcagg
     4381 aacgcctgag gaaatcactg aagtggaaga atcatataca ggccgttatt tgaagcctgt
     4441 tatcgaacgt gacaaaacac gcatgaaatc gctcttgaaa gcgaaagaaa cagctacatc
     4501 ttaaaaccct ctgttaagag gggacagctt gtcagcaagt ccatccttgg gcttagcagg
     4561 caagcttttt ctttacggca taataggttt agaagtgaac ttactgttca agcaggaaac
     4621 acaggtacac aacaagcaga agatcttggt gcaattaaag atgaaatgga tgcgcttatt
     4681 gaggaagttg atggcatttc aaatcgtact gaattcaacg gcaaaaagtt attagatggt
     4741 actgaaactg atggtttcac tttccaaatt ggagcaaatg ctggccagca gcttacagtt
     4801 aacattgaca gcatgtcatc aactgcttta ggagtaaacg cacttaatgt aacagatttt
     4861 gctaatactc cttttgatac tcaacttgaa agtattgata ctgcaatcaa caatgtctca
     4921 aaccaacgtg ctaaacttgg tgcggtacaa aaccgtctag agcacacaat caacaacctt
     4981 ggtgcttctt ctgaaaactt aacagctgct gagtctcgca tccgtgacgt tgacatggcg
     5041 aaagaaatga gcgatttcac aaagaacaac attctttctc aagcttctca agcgatgctt
     5101 gctcaagcaa accaacagcc gcaaaacgtg cttcaattgt tacgttaatt ttgaacggct
     5161 atctctttct gatagttaca gcttgccgac agagtccatt aactggatgt gccggcaggc
     5221 tttttttgtt acaatcattt tgtgacagcc agggaatcac ttgttttttc aaaacaaaaa
     5281 aggcaagata ttgtcatcta acctttaact ttgtttcagt tataatgaat gatatgtttt
     5341 ccgcaattac aaagtcttct tatacagctg cttacttaca ctcattaaat cttgaacaat
     5401 atgcgtgttg ctgctgtcga gcagcacttt tatatattgg atatcgtgag agatcgggaa
     5461 gggtccgtga atctgctcaa gctcggcgat catttcacga ttcattcttt tttgttcatg
     5521 aatcagcttt aacactgatt cttgtgttac tgtatagtca ctgggatctt tctccattgc
     5581 ttgtccagcc tccttggttg ccgtaagctt tttacatatt gtaatcggta tagccggaag
     5641 attctacttc taaaactttt taaaatccaa tttccgtaaa tcggagcatg tctaaagaat
     5701 gaatttgaaa cctgaagaga ttttaaacgt ataaataagt aaaacgtccc tgatcttttg
     5761 tcatggacaa ttcacaatag gagtgtggaa tgaattgaac cgtaatcaag ctattattgc
     5821 atcactttgt tatttcagtg tgtttattgc gccaatcatt gtgccgattg tcgcttattt
     5881 tgttgtaaat gagaaagaaa caaaacgcca tgcgatacgc tctcttattt ctcacatagt
     5941 tccgtttgtc ggctggctgt tcctttttat tgctttactt ggcggagccg ttgcgattga
     6001 tggagacagc ttgcttcctg tatttgtaat catcggggga gcggttattt atttcttagt
     6061 tgtcattggc attatcattt ggaatgtgat tcagggcatt aaggtcctgc gtgaagcata
     6121 agtagaaaat ggggaggaga tcgtaatgaa gcaagaaaag gaacgaatcc ttaagcttgt
     6181 tgaggaagga aagcttacag cacaagaagc tttgacactg attgaaaagc ttgacagcga
     6241 ctacaaggaa aaagaagaga aaatcacagc gctctcagtt cacgttcatg acgaggaaga
     6301 gccatttaca acggcaaaaa aagaaagcgg caagccgtct ttaggagcca agctgtttga
     6361 ctggattgat tctgctgtaa aaaaggtcaa ggaagttgac cttgatttaa actttggcca
     6421 tgcgtatgat gttcagcaca tttttcaatt taaagatact gacttttcca gtgttgagct
     6481 acaaattgcc aatggcagcg tgaatatcgt tccttgggaa gatgacgata tcagagcgga
     6541 atgccaggcg aaggtatatc gcgctgatag ccaggatgcg gcgagacacg catttcttca
     6601 gcatattgaa tgtgaaatca aaggaaataa gttttttatc cgtactgaga aaaagacgat
     6661 gaaaacgaat gtcactttgt atattcctca gaaggagtat gataagattc gagtgaagct
     6721 gtttaacggt ccggtcagag gggaacattt acatgtaaaa gagttctcag cgaaaacgac
     6781 aaacggcgtg ctgtcgttct cgcacttaac agccgaaaaa gccatagctg aaacggccaa
     6841 cgggcaaatt aagctcgcca gccacagctg cggaacgata gaagcggaaa ccattaacgg
     6901 attgatcgat ctcagaggga aaagcgaatc cattgacgtt caaagcttta acggaaatat
     6961 tgcgattaac gtaacagaat cagactgccg ctccatttat gcaaaaacaa cgactggaag
     7021 tgtggagctt gcgattccgg acgatctcgc agtgaaggca gagctgaaaa gcaatcttgg
     7081 ttcgctgtcc catgaattaa tggatgttga gatgctgaag gaaaaaaatg ataccattca
     7141 aaaagaaatg atgttcactt caaatcaggc acatgatcaa aacatcaccg tcttctcaga
     7201 atcactaaca ggcgccatca aactaaagta ctcacagagg tgatcgaatg aataagcttt
     7261 atcgctcaga aaaaaataag aaaatcgccg gtgttatcgg aggtctcgcg gaatatttca
     7321 attgggacgc atcactcctc agagtcataa ctgtcatctt agccattatg acaagtgtct
     7381 tgccggtttt gctcatttat atcatttgga tttttatcgt cccgtcagaa agggatatga
     7441 aatgacatgg taaaatgggc agtcagcatt ttagtcaatg cattgttatt aatcgtcatt
     7501 gatggatata ttgactccat ccatatcagc agcattgggg cagcgattat cgccagcctc
     7561 attttatcga tcttaaacgt gctgattaaa ccgcttttaa ttatctttac actgccggtc
     7621 accatggtga cgcttggact cttcttgttt gtgattaacg ccatcacgct tatgatgacc
     7681 gcgtccatta tgggtgacag ctttcaaatc gacggcttcg gcacagcgat atgggcatcc
     7741 gttatcctgt cggtcttcca tttgctgatt caaaaaggca ttttagagcc gcttagaaaa
     7801 aaataaaaaa agctgcccgc aaaggcagct tttttacatg tggtgctttt tttgtttcgt
     7861 gcgcttctgc ggcgaaaaca ttggcggaag aaggcaaaag ctcaatgcgg caaatccaag
     7921 gcctaggagg ctaaaggaca tggcagacgg catcattgtg agcaagtagg cagataccat
     7981 cggacctgct gccatgcctg tatatcggat aaagttaaac attccgattg cactccctct
     8041 gttgtgctca aattcattag taatcattgt agagtacaag ggaggaataa cccccatcga
     8101 gatgccaaac agtgccagcg tcagagccat gagaacgaga gaaacggaat gtgtaacagc
     8161 aaataaaatg atgctgcagg cggcaatcac attgcttccg ataaacaagg tgtgcagccc
     8221 gatttttgcc tgtattcttt taaacagaaa cgtacctgca atcgtgctca gcgccagcgg
     8281 caaatataac agtcctgcta tacccacatc tatatggtaa tgctctgtca gcaatattgg
     8341 gaggtagaca attactgcaa aataaatgaa aaagagaaca aaacttaaag tcaaaatgac
     8401 gttccctgtt cgatttgtaa atattgattt ataatgagcg aacacattgc ctttggcttg
     8461 ctgcatagat tcagttggag aatctttagg aaaggtgatg ctgtttgtca ccagcaaaac
     8521 gatcgagatg gccgcaagta tccaaaaaat cccttcgtat ccggctgctc cgcctatgaa
     8581 tcctcctaga actggggcaa tagccggtgc gacagacagg agcatttgat acgttcccat
     8641 tgcactcccg cgttcattgc ctgtaaacag ctgtccgatc gttgtggcag caataagagg
     8701 cagtgctgcg gaaccggctg cctgtatcat tctgaatatc agaaacaaag taaagtcagt
     8761 agtcaccgca cagccgatgc tggctgctgc cgttgccaga ataccggtaa tcaagacgat
     8821 ccgagcgcct ttaaaatcaa tgatcgctcc taatataatt tgcattattg ctgtcacaat
     8881 cataaaaact gagactgaca ggttcaccat agctgtggaa acatggaatg attctttaat
     8941 gatcggaaga ataggtgaat aaatgttctg gcttaaagat gcaaaaaatg cactgaagca
     9001 aacagtgtat aagatcaata ctgcctttga ttttgtatga gccaaagtgt acaccgcctt
     9061 tttgtttact ttacaggttt gtccggagtc ccatcggttt gttctgcttc aagtgttgac
     9121 gtcaactgct ctagaaactt caatacagcc ttttgttctt cttgtgaaaa tgaatcgagg
     9181 aattggtaaa aagccagctc ctttttttta tgcagtttct catgtaagtc gaaaatccgt
     9241 ctgcctttac gggttaattt aaaataaact tcttttttgt tatctgtcag ctgatagctg
     9301 ttaatgaatt cttccttgat cagttttgtg ctgattttcg ttacattcgc tttcgataag
     9361 ttcatttttc tggcaatccc cgcattgtta atgggttcat gctggccgat gcagtcaatg
     9421 acgtgaatgc ttgtcatatt tttgggaatt tcagctacat cttcttctgc ggcctcctta
     9481 aaaaattgct gaagtaaagt gttcgctttt tgttcattaa ggtgcagcag tctcacgtat
     9541 atgtcgtata tcatctgttt tgtcaaatca gacataggct accttctttc ttaggaataa
     9601 tagtttacga tgaaacaata aaacaaatcg tttactggtg aacaatttta ttttatcttt
     9661 tcttgttttc gttgtcaatt gttggtgttt agactatttg ttgatatgtc atgctgaata
     9721 tgcgtgtaaa aagtgtgtgg aaaatgtgat gtttacattt attttcaaat atttgggcac
     9781 agatcatatt gacgtttgga aggaaaaggg ataaaatgac taatgtttac tagtaaacat
     9841 ttatcatgat atgggtattg aaaatgatta ttaaaatctt aaaaaacatt tggaaacttt
     9901 aagtgatgcc tgccattcgt cttgttcagt tgttctagct attccatctt gtatttgagg
     9961 gtgatgactt aaagcgggat aagttttagg ggtgaatgag atgaccggaa tggtaacgga
    10021 aagaaggtct gtgcatttta ttgctgagcc attaacggaa aactgcagag aaatatttga
    10081 acggcgcagg catgttttgg tggggatcag cccatttaac agcaggtttt cagaggatta
    10141 tatttacaga ttaattggat gggcgaaagc tcaatttaaa agcgtttctg ttttacttgc
    10201 ggggcatgag gccgctaatc ttctagaagc gcttggaact ccgagaggaa aggctgaacg
    10261 aaaagtaagg aaagaggtat cacgaaacag gagatttgca gaaagagctc ttgtggctca
    10321 tggcggggat ccgaaggcga ttcatacatt ttctgatttt atggataaca aagcctacca
    10381 gctgttgaga caagaagttg aacatgcttt ttttgagcag cctcattttc gacatgcttg
    10441 tttggacatg tctcgtgaag cgataatcgg gcgtgcacgg ggcgtcagtt tgatgatgga
    10501 agaagtcagt gaggatatgc tgaatttggc tgtggaatat gtcatagctg agctgccgtt
    10561 ttttatcgga gctccggata ttttagaggt ggaagagacg ctccttgctt atcatcgtcc
    10621 gtggaagctc ggtgagaaga tcagtaacca tgaattttct atttgtatgc ggccgaatca
    10681 agggtatctc attgtacagg aaatggcgca gatgctttct gagaaacgga tcacatgtga
    10741 aggatgatag ggggagtaaa acatgagcca atcgattaaa ttgtttagtg tgctttctga
    10801 tcaatttcaa aacaatccat atgcttattt ttcacaactg cgggaggaag atccggttca
    10861 ttatgaagag tcgatagaca gttattttat cagccgctat catgatgtcc gctatatcct
    10921 tcagcatccg gatattttca cgacgaaatc acttgttgag cgtgccgaac cagtaatgcg
    10981 aggcctgtac tggcccaaat gcatggaaaa gaacactctg ccaaaagaag aattgtagtg
    11041 agaagcttca tcggtgacgc actggatcat ctgtctccat tgattaaaca aaatgcagaa
    11101 aacttgttag cgccctatct tgaaagaggg aaaattgatc tcgtcaatga ttttggaaag
    11161 acgtttgcgg tgtgcgtcac gatggacatg ctcgggctgg ataaaagaga ccatgaaaaa
    11221 atctctgagt ggcacagcgg agttgccgat tttatcacga gtatctctca atctcctgaa
    11281 gcgcgggcac attcgttatg gtgcagcgaa cagctttccc aatacttgat gccggtcatt
    11341 gaagaacgtc gcgtcaatcc gggatcagat ttaatttcca tcctatgtac ttctgaatat
    11401 gaaggcatgg cgatgtcgga caaggatata ctcgcactga ttcttaatgt gctgttagcc
    11461 gcaacggaac cggctgataa gacgctggca ctgatgatct accatttgct caacaatcct
    11521 gagcagatga atgatgtttt ggctgaccgt tcgttagttc cgagagccat tgcggagaca
    11581 ttgcgttata aaccgccggt tcagctgatt ccgcggcagc tgtcccaaga tacagtggtc
    11641 ggcggtatgg aaatcaaaaa agatacgatt gttttttgta tgatcggtgc ggctaaccgg
    11701 gaccctgaag catttgaaca gcctgacgtg tttaatattc atcgggaaga tcttggcatc
    11761 aagagcgctt ttagcggcgc cgcccggcat ctcgctttcg gatccggcat tcataactgt
    11821 gtaggagcag cttttgccaa aaccgaaatc gaaattgtag ctaatattgt gctggataag
    11881 atgcggaata tcagattaga ggaagatttt tgttatgctg agtccggtct gtatacacgc
    11941 ggacccgtct cacttcatat tgtatttgac agggcttaat aatatggaaa ggtctctccc
    12001 gtgcgggtga ggctttttta catatgcttg atccacaagt taataaacgc agtcactgtt
    12061 ttcaactcat caattgaaaa ttcattgaag agctgtttgt attgctcctt tgctttttga
    12121 tgaagccgtt catgtaaccc cgccagttct ttgccgcttt cagttaacgt ataaaaaatt
    12181 tcttttttgt tgcccggctt ttttgtcact gtaatgatgt tatgtttcaa caaagcttga
    12241 attgcttttg aaacagctgc ttttgagata tgtaagcggg atgctaagaa gctgttattc
    12301 acatttttgc tggtatgtaa acaggaaaca atgtgaagct gggtcaatgt ccagtctttt
    12361 ttgaggtggt cgagatgttg tgaggacgga atctgcacct tttgccagac gcgtttatct
    12421 ctttttaatg cgaattgttg tatagcttga aataattcat catgaatgct gttgttcatc
    12481 aatttgcctc tctgatttaa ttgttaactg gttaactatc cttagtttac tggggaatag
    12541 tggaattttc aagaggcaga cgtttttttg ttctttatgc acaaaaatga actcataagc
    12601 ttccctgttc agacgcaaaa tacatgaaaa gggaggtgat ttgatgagga aatacacggt
    12661 tattgcttct atattactgt cttttctttc cgttttgtct ggcggccatc atgaatcaaa
    12721 agcttttcct gttgtacagc aggaactatt tcaaacgcct cattacatac ccttgcttga
    12781 aaatcctcct caaattgatg tgaaagctga aatgagaaac aatctaatga ttgaggctca
    12841 agttcgcgat aattcgtatc aagcattttc cgcctttctt ttttataaac aaagtaatga
    12901 gctgggctac aaaatggtgc cgatggaccc gacccccgga gcggtccgca agtttttggc
    12961 acaaattccg aaacgtttga tttggaacag cgagcttgag tattacattg tgatgtcgaa
    13021 tgggaagcag cgggtggagt cggagacgaa gaaactgaag cttgaaggat accaggctga
    13081 ccttgcccat attccggagc ttctgatcac agagctggcg gttgatacga agaatatagg
    13141 acgtgcagac ggttatgaat ttattgagat ttttaatacg actgacagaa cgattgattt
    13201 taaagactac catttccgtt accgctaccc aaaagaaggg cctgactccg atctgatttg
    13261 gagacctgat gaaaggatct tgataccatc cggcgaaacg tttgtcgtct ggctgaagcc
    13321 tgccgggcat ccagaattga cgtcagagga tttcaatcgt tattatcaga cacaactaaa
    13381 agaaggaaaa aatctcgcgg tgatagacga gacagagggt atggccaata cgaggccgcg
    13441 ggctgttgtg atatcaacga atacaggaaa ggacatttcg gttgcgcatt atcggaaaca
    13501 tgcgctgcgc cggctgtcat cagtgcttta taagtatccg ttgaacggaa ccgcagaact
    13561 tctgaacatt tcaatcgggg aaaagaatcc gtctcccgga acggtattac aggcacaggt
    13621 gcccgagcag aaacggaaaa tcaagctcga taaagaaaag ccggtcattg aggatttgac
    13681 tgaccgcaag cctgtccgcc ctgctgaaag cattgagctg cgggctgaca tacgggaccg
    13741 aagtctagtg aaaacagcgg ccttttacta ccggacggat gagaacaaac cgtttaagcg
    13801 aattcttgct gaaaaagacc gcaacgataa tctctaccat tatattgtgt attcgcctga
    13861 attgatcggg aaggatcagc tagagtatta tgtggcggcc ggggacggga tcaatgaagc
    13921 gagaacgccg gtcaaaatga ttgatattaa acaaacaagc aagtcgcacg gtctgcggat
    13981 gaatattgaa aacagagaca cgctgtcggg aactcaattt ctcaaagcca cgacagaagg
    14041 gcgagctgac cgcattaagc tatggattga cgggaaaaaa caagtgacag agcccgcgat
    14101 ggaaaaagaa gtatattttg cgttcgatac gaggaaaacg aacctttatt ttaaaaacgc
    14161 ggtaacgatg gaaggaaaag tgctgaaggt gttcgatgat acaacaaata aataccgcac
    14221 ctattctgtt ccattgccag agacactgct tcggaaaggg aagcagctgc agcgtattac
    14281 aatacgatcg ggttcgaagg tgtctccgtt tgatacggct gaaaaccgtg atgacttctt
    14341 agtcaaaaac gcccgattgg tgttatcgga tggaacggtg atcagggatc agagggtgtc
    14401 tcctgagaag gaactcttta ttggcgataa tcagcgctcg aataaaagct ggcaatttca
    14461 gttcaatctg cctgacggac tatttacatc acagctgctg gagtgggaca catcaaagct
    14521 ttctgaaggc gctcaccata ttcaagcaag cgacggcaag gaaaatgtca gcctggttgt
    14581 acgggtagat aattcaggac ctcacattga gccgaatatc accgaggggc aaacgtataa
    14641 gggaaacctt atcctgcagg ccgatatgta tgataaatgg agcaaaattg aggaagcgga
    14701 ggcgtcttta gatggagaaa gcattacact cccatatcat acgtcatctt ctgacctttt
    14761 gccgggaaaa cattctctaa aagtgacagc aacggatctc gcgggcaata aagcagtgat
    14821 tgagaggacg tttaaaaccg aacgagagca tccggaccag ccggaggtta tagacagcga
    14881 tgccgacacg cataaggcca agctttctgt ccgggtgaaa gatccgacga atgatgccat
    14941 ggatgttggt ttttataggg gatttcaata tacagcgcga aatcatgtga aaatatttaa
    15001 gcacgcgtca ctgactgaac cgccgaaaag ctttgtgccg gaaagggaga ccccatttac
    15061 gaataaagaa ttggagcggg tatcagctgc tgatggaaaa acagtatcga cagaaaacaa
    15121 agagcttttc ccttatcatc gctttgaagt gacggttgat ccatcaattg atgagaatga
    15181 tctggcagaa gcggtttgga agggcagttc tttgccgggg agaaaggtga cgatgtatgc
    15241 ttggaattat cggacaaacg aatggcagcc tgttgacagc ttcgtcgcga aggatgacaa
    15301 accgtttacg ctgaaagcgt ctgtgattgc cgccgatttt gtacgggagt cgaaaatgaa
    15361 tgtgattgta caggatgaaa ttcctcctgc gaaggatatg tacacatttg tctggatgtc
    15421 tgatacacag tattacgccg aaagctatcc gcatattttt gataaacaga ccgaatggat
    15481 aaaggataat caaaagcagc tcaatatcaa atatgtgttc cacacgggtg atattgtaga
    15541 tgattccgcc gatatcagac agtggaaaaa cgctgatcgg tcaatgtccg ttctggataa
    15601 gaacggaatt ccttacggcg tgctggccgg caatcacgat gtcgggcata aggacggctc
    15661 ctacagggcg tttggcaaat attttggcag cgatcgtttt gataaaaaat tgcattatgg
    15721 cggttcctat aaaaataacc gcggacatta tgatctgatt tctagcaacg gcaatgatta
    15781 tatcatgctg tacatggggt ggggcattac ggatgaagac atcgcgtgga tgaatcaggt
    15841 gctgaagaaa caccctgacc ggatggcaat tttagctttt catgagtatt tgctggtcag
    15901 cggaaacaga agcccgatcg gagagaaaat attcaaagaa atcgtaaagc cgaacccgaa
    15961 tgtcgtgatg gtattgagcg ggcattatca tagtgcgatg agaaaaacag atgagctgga
    16021 tgatgatggt gatgggaaac cggatcgtct tgtgcatcaa atgctggccg attatcaggg
    16081 cgggccagaa ggcggtcaag ggtatttgcg tttgcttcaa tttgaccagg ccaatgacat
    16141 ggtgcacgta tcgacgtatt ctccgtatgt aaaggataag aattactatg atacggatac
    16201 ctatggcaac aaggatgaat tttccctttc gcttgatttg aagccaagaa tcaaaaaggt
    16261 ggaaaccgat tattttgaat gtaacgtata taccaatgaa gagctcggaa aacgggagca
    16321 agtgaagagc ggggatacag ctgaattccg ctgggatcat cttgaaccgc agtctgttta
    16381 ttattggtac attatagtgg aagacagttt taacggcaaa acgaaatcac cgatctggaa
    16441 gttcaagacg aaaaaagaaa catatcgtcc ggcaccagac cagtttgatt ttcgccatgt
    16501 ctccaatcct taaaaagggt tggaggcttt tttatgaaag acgatccata tagtggacaa
    16561 atgtataacg gtcgcctctg tatacggatt ttgcatgctc aaaagcagtt ccgttctgca
    16621 gatatgttgt tcgtttaatt aataggactg gcgctccctt ttgaatacca agaatgttcg
    16681 cttcttccgt ggtggcagcg cttggctcaa gctcctgttt tgcacgggaa atcggtatgc
    16741 tgttatacct ttcaatatga tcatagatcg acgactgaaa atgcgattcg ttcaattcac
    16801 ccgcaagctc aaagggaata tgtgaggact caatcgccat tggaatatca tttgccagcc
    16861 gcacccgagt gattttatgg atagaggagg ggtgcccgca gcctaatata gccgcgagct
    16921 cctcagttga atcaataagc tgataatcaa tgagcctgct gcccggtgtc atcccgcggc
    16981 ttttcatatc ctcggtaaag cttgtcagcc cttgaagtgc ttgttccatt tttggcttgc
    17041 tgacaaaggt gccccgccct ttcaggcgat agagcaagcc ttcattaact aaattagaaa
    17101 gcgcctggcg aactgtcatc cggctgatcc cgaattgttc ggcatattcg cgctcagaag
    17161 gaagaggcat atccggctgc agctctccgt tcttaatttg ggtttttaat tgctccataa
    17221 tctgatagta aatcggaata ggcgattgtt tattgatatt cataagtcag catgttcctt
    17281 tcaaggtctt aatgacgcgg cttcacgatc aatcaaaacc gtaacatcgg aatgcaggtg
    17341 caaagcagat gcgggaaaat cttcgcttat gtttccttct aacagcttac gaacagcttc
    17401 cgctttgctt tttccagaaa taagcagcag aatgcgcttg cttgagagta tcgtttgaat
    17461 tcccattgtc agtgcttttt tcggcacgct gtctatggag ggaaaatatc tggcattcgc
    17521 ttggcgggtt tgctcattta atgtcacaac atgagttcgc gacttgaaag acgttcccgg
    17581 ttcgttaaag ccgatgtgtc cgtttcggcc gatgccgaga agctgaatgt cagtgccgcc
    17641 gagggactcg actagctgtt cataccgtct gcattcagct tccaaatcgt ctgcatttcc
    17701 attcggaata aaatgccggc taggcttgct gtcgatatgc tgaaaaaaac ggtcgttcat
    17761 atagaagtga tagctgttcg ggtcatcgct tgaaagtccg gcgtactcat ccaaattaac
    17821 tgtagtgacg ttttgaaatg agagattctc agtttggtgc aggcggatca gctcgcgata
    17881 agtgccttcc ggtgtgccgc ctgtcgctaa cccgagaacg gcatcaggtt tttccttgat
    17941 tgtatctgcc gttattctgg ctgctttttg gcttagctct tcatacgttt gacattccat
    18001 tactttcata tctggtcagc ctccttggat ataaatgcaa tgtttccgcg gcaaatggtg
    18061 agaatcacct cgcaatcact gctgacaatc actaaatctg catcttttcc cacagtcaca
    18121 ctgccttttc ggtcaaagat gcccagctgt ttggccgcgt ttgcagaagt tatattagct
    18181 atatccgtcc aagagcaatt tgtaaactca cgcatatgcc gtgcaccttc gttcattttg
    18241 aggatcgaac cggcaagggt tccgtctgaa agaagagctg ttcgtcctct taccgtcaca
    18301 ctttggccgc cgaactcata aacgccgtct ttcagtcctt ttgccctcat ggaatcggta
    18361 attaaaatga gcttgctgct gcctttcgcc aaaaaagcaa gcttagccgc taaaggatgg
    18421 gagtggatgc catcggctat tagctctgtc acaaacccat catgggccag cgccgttccg
    18481 atcactccgg gttctcggtg atgaaacggg ctcatcgcgt tgtacagatg ggtcatatgg
    18541 ctcgcccctg cttttgcggc atccgacagc attgcggaat cggcatccgt gtgccccatg
    18601 gacgcgataa tcgattcttc tttcaaatgg cggatcagtt caaaatgctg atcctcctcc
    18661 ggcgcaagtg taacaatttt gatcagtccg cccgcctcct gctgccactt tttgaaaagc
    18721 gcaacatccg aaggcctaat ccactccttt ggctgcgctc cggctctttt aggcgaaaca
    18781 aaggggccct ctaaatgaat gccgagcagt tctgcaccta ataaagagga ctcttcagcc
    18841 gctttccact ctcttgcgtt caccaaagct tgagaaatgt tcccatgctc ttgggtaatc
    18901 gttgtggcta aaaagctcgt cgtgccttct tcgggcagtc ttgacgacat gatgtcgagc
    18961 gtggaaaagc tagcatccat tgtatctgcg ccatagccgc cgtggatatg aatgtcaatc
    19021 atgccgggaa gcaaaacgga atccgcaggt gcttgaatct cttttgaata tgactccttg
    19081 ggccgttctg ttgagactgt gctgattttt ccgtcattga ttccgacata gccgtttttg
    19141 attacttcat tttctgtcac gatcgcaatg tctttgataa gaagactctc tgccatagat
    19201 gatccgcctt tctggaattt gaataccttt attttactct ttttcagact agtgatctag
    19261 accagctgag gtgttttccc gccttttata aaatctgttt taggttcaag cctgaaagtg
    19321 ataaaatgaa agtattgtga gcaagctcgg ggcagcccgg aacaggagga acatatcgtg
    19381 gcaaaggttc gcacaaaaga cgtaatggaa cagttcaatt tggaattaat cagcggagaa
    19441 gaaggaatta accgcccgat tacaatgagt gacctatcaa gaccaggcat tgaaattgcc
    19501 ggatatttta catattaccc gagagagcgc gtgcagcttc tcggaaaaac agagctttct
    19561 ttctttgaac agcttcctga agaggagaaa aaacagcgaa tggattctct gtgcactgat
    19621 gtaacaccag ctattattct ttctagagat atgcccatcc cgcaggagct gattgacgcc
    19681 tccgagaaaa atggggtgcc tgtcctcaga tcgccgctga aaacgacaag actgtcaagc
    19741 cgtttaacca acttccttga gagcaggctg gcgcctacca cagcaattca tggtgttctt
    19801 gtcgatatat atggtgtcgg cgtgctgatc acaggaaaga gcggcgtcgg aaaaagcgaa
    19861 acagcgctgg agcttgtgaa aagagggcat cgccttgttg ccgatgattg cgttgaaata
    19921 cggcaggagg atcaggatac tcttgtggga aacgctccgg aattaattga gcaccttctt
    19981 gagatcaggg gccttggcat tatcaacgtg atgacgctat ttggcgctgg cgcggtcaga
    20041 agcaataaac gaatcacgat tgtcatgaat cttgaactct gggagcaggg caagcaatac
    20101 gaccggctcg gacttgagga agagaccatg aaaatcattg atacagaaat tacaaaactg
    20161 acgattcccg tccgcccagg ccgaaatctt gcagtcatca tcgaagtggc agcaatgaac
    20221 ttcagattga agcggatggg gctgaatgcc gctgaacaat ttaccaacaa gctggcggac
    20281 gtcattgaag acggtgaaca agaagaatag gagttggcgt taaatgaatg aagcgataga
    20341 accactcaat ccgatagcat ttcagctggg gccgttagcc gtccattggt acggaatcat
    20401 tatcggtctc ggcgctttgc tcgggctgtg gattgcgatg agggaaagtg aaaaacgggg
    20461 gctgcagaaa gatacgttta ttgatcttgt cctgtttgcc attccgattg cgattatttg
    20521 cgcgcgaatt tattacgttg cctttgaatg ggactattat gcggcacatc cgggagaaat
    20581 cattaagatt tggaagggcg gcattgccat tcatggcgga ttaatcggcg ccattttaac
    20641 gggttatgtg ttttcgagag tgaaaaaact ttcgttctgg aagcttgcgg acattgccgc
    20701 gcccagcatt ttgctcggcc aggcgatcgg ccgctggggg aacttca
//
