LOCUS       NZ_JAQOXM010000001     21612 bp    DNA     linear   CON 10-MAR-2024
DEFINITION  Bacillus subtilis strain CWTS 5 NODE_1_length_1063700_cov_36.2731,
            whole genome shotgun sequence.
ACCESSION   NZ_JAQOXM010000001
VERSION     NZ_JAQOXM010000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 1063700)
  AUTHORS   Natarajan,D.A., Chandwani,S. and Dewala,S.
  TITLE     Complete genome sequencing of Bacillus subtilis (CWTS 5), a
            siderophore-producing bacterium triggers antagonistic potential
            against Ralstonia solanacearum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1063700)
  AUTHORS   Natarajan,D.A., Chandwani,S. and Dewala,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2023) Molecular Biology Unit, Uka Tarsadia
            University, Bardoli, India, Maliba campus, Bardoli, Gujarat 394620,
            India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-JUL-2022
            Assembly Method                   :: SPAdes v. 3.11.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 02:26:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,268
            CDSs (total)                      :: 4,150
            Genes (coding)                    :: 4,020
            CDSs (with protein)               :: 4,020
            Genes (RNA)                       :: 118
            rRNAs                             :: 10, 12, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 11, 6 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 59 of 130
            Pseudo Genes (incomplete)         :: 77 of 130
            Pseudo Genes (internal stop)      :: 19 of 130
            Pseudo Genes (multiple problems)  :: 23 of 130
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:37:08
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 368775
            Orig. end                         :: 390387
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAQOXM010000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21612
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="sactipeptide"
                     /region_number="4"
                     /rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /tool="antismash"
     cand_cluster    1..21612
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /kind="single"
                     /product="sactipeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21612
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:11612](-)"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..11612)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="1"
     gene            complement(421..540)
                     /gene="phrF"
                     /locus_tag="PO770_RS01890"
                     /old_locus_tag="PO770_01895"
     CDS             complement(421..540)
                     /codon_start=1
                     /gene="phrF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391627.1"
                     /locus_tag="PO770_RS01890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_01895"
                     /product="phosphatase RapF inhibitor PhrF"
                     /protein_id="WP_009968329.1"
                     /transl_table=11
                     /translation="MKLKSKLLLSCLALSTVFVATTIANAPTHQIEVAQRGMI"
     gene            complement(524..1669)
                     /gene="rapF"
                     /locus_tag="PO770_RS01895"
                     /old_locus_tag="PO770_01900"
     CDS             complement(524..1669)
                     /codon_start=1
                     /gene="rapF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391626.1"
                     /locus_tag="PO770_RS01895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_01900"
                     /product="response regulator aspartate phosphatase RapF"
                     /protein_id="WP_021480843.1"
                     /transl_table=11
                     /translation="MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEE
                     DQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPRLSDLLLEIDKKQARLTGLLEYYFN
                     FFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFSMDY
                     ARQAYEIYKENEAYNIRLLQCHSLFATNFLDLKQYEDAISHFQKAYSMAEAEKQPQLMG
                     RTLYNIGLCKNSQSQYEDAIPYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKA
                     HEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAIQGFFNFLESKMLYADLEDFA
                     IDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV"
     gene            1672..1902
                     /locus_tag="PO770_RS21340"
                     /pseudo=""
     CDS             1672..1902
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009968328.1"
                     /locus_tag="PO770_RS21340"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MQIPPFIILYMSYFIINILVRSAKKPQFFLSLHRMFGDIFSKRSV
                     YPQ"
     misc_feature    complement(1702..1718)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 22.81"
                     /tool="antismash"
     gene            1899..3254
                     /gene="ywhK"
                     /locus_tag="PO770_RS01900"
                     /old_locus_tag="PO770_01905"
     CDS             1899..3254
                     /codon_start=1
                     /gene="ywhK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391625.1"
                     /locus_tag="PO770_RS01900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_01905"
                     /product="YncE family protein"
                     /protein_id="WP_041850408.1"
                     /transl_table=11
                     /translation="MRKNKLSFKEKAGAEQEECLCFCEGEASREAMFQAPIELPEGFVV
                     DPAEAVANVTWNADSLSCVSDRCLIQTGPEPDEVGVQFAVRLQGTVTLLVSVSPVRNQY
                     GQGDGAVSVIHNAEIDQVVYYSDEPDDCPDISDITIENLVVVPPFYGSPLSVTGTIVLV
                     PEPEPVYAFTANPNDQSVSVIDTNTHTVVTTIALPYNPAGIEITPDKSAVFVLHPNNNV
                     ISVIDYDTLTVTATILLDQPPRLIRFIPNHEFAYVFTGTAIYVIGIDTLTVERSIPVEG
                     FDIAIDPNGLFAYVLNFGLVQKVDLSTGEVLGTIERELIVSSIETDSTERYAYVLEQEF
                     LFNYLTVIDLNTFTIRDTQELEPDGLYVMFTSGSEVYLHDSFTGNLYSVSPNGAGVIGN
                     LPQSATDYAFTSNGDFLYATRFIEQSIIVYNTDDYSEETVISLRVSPGAITI"
     gene            3293..4669
                     /gene="ywhL"
                     /locus_tag="PO770_RS01905"
                     /old_locus_tag="PO770_01910"
     CDS             3293..4669
                     /codon_start=1
                     /gene="ywhL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391624.1"
                     /locus_tag="PO770_RS01905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_01910"
                     /product="YncE family protein"
                     /protein_id="WP_041850409.1"
                     /transl_table=11
                     /translation="MKKNDLSALQENCFCFCDEEISREAPFQVPIDIPEGFKVDSAEAS
                     AAVTWSTDNLSCISEPCLIQTGPEPEDIGVRYAVRVQGTITLLVSVSPVRNQYGQGDGA
                     VSVIHTEEIDQVVYYAAQSDRCPDFSQMTVEDLLIVPPFYGSPLTVAGTLVLPPSPDPQ
                     SYVFTANTGDSTVSVIDADLNTVVKTIPFSDVPTNLGVTFDKAFTYVLHGNTNLVSVID
                     NKTLTIINTITVGGGPRKIEFDPTDEFAYVMAAGSIYVINTASQSVIDVIPIPGALDFA
                     LDPNGQYVYTANGSSWSVDKYDVNTGQLVESIIDSFEFPSLIATPYAGNFAYVLNGELW
                     PKGVTEISLSPLSRGGDFSRLFETLRTIVFSLDSTRAYFLEPYSEPFLINNLYVVNTAR
                     QRIIANVSLPGAFDLAVTPDKQYIYAAQPNDNAVTVYRTSDYTAVTVIPVGAGPSAIAM
                     "
     misc_feature    complement(3309..3326)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 21.71"
                     /tool="antismash"
     gene            complement(4675..5376)
                     /gene="albG"
                     /locus_tag="PO770_RS01910"
                     /old_locus_tag="PO770_01915"
     CDS             complement(4675..5376)
                     /codon_start=1
                     /gene="albG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391623.1"
                     /locus_tag="PO770_RS01910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_01915"
                     /product="antilisterial bacteriocin subtilosin biosynthesis
                     protein AlbG"
                     /protein_id="WP_041850410.1"
                     /transl_table=11
                     /translation="MKKNTVFTLLLLLIGMAAYSFGWVQAAAEAAAQYVQMINNDAVRL
                     GLLACTAALLMLPAFFYLHYVTQSVKNMTAAFQKLTQSHQSCCDFQQHNLCSRYAEDVK
                     SLRDSYKNVRQTYVMAAVLCQVIIFGCMFEIVKAVPFRLHTPPALSMGLAMLLILYLLF
                     CMRTYLRQLFRHGSLFRKVFAGVLAAAGIWWMLSFSISELLFLIILAAIQQIGSFIYKR
                     FSYRSTASLDL"
     gene            complement(5373..6653)
                     /gene="albF"
                     /locus_tag="PO770_RS01915"
                     /old_locus_tag="PO770_01920"
     CDS             complement(5373..6653)
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="albF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391622.1"
                     /locus_tag="PO770_RS01915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_01920"
                     /product="pitrilysin family protein"
                     /protein_id="WP_041850411.1"
                     /sec_met_domain="Peptidase_M16 (E-value: 6.3e-14, bitscore:
                     48.2, seeds: 24, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKKAFFQQLDERTDIRYTDSGLKIFRLKFPRAHLRLCNVKIDFG
                     SRDVCIRAESGDTLLPYGTAHFLEHLLFWHNGRNLYSDFFAHGALLNAFTTYTDTNFMF
                     TSLPDRLRQTIPILLDALWNHSFDKKIVAQEKAVITSEIQTAHLNHQLSYHYQLISMLS
                     PSSPAAVFPAGRIEDIEALDINDLQKAYKAAYQAHRMTLFLIGGSENTETLLPPHLQLE
                     KRPDYHAERKIIPACPPALSQKMVLGDEERMEDTWTGLQIGALPGQNDLLSIKLYWDIA
                     ARILFQLDSPFFQEIQQTYRLEIDRLSAETYIYEDGGFLILHSQGTHSSAYIDVASYYV
                     TQKKEQVAAWLQYGKDSLTDAIIYDSDYVRKCFEWAAECDRCDCSFLDMYHIIQDMDAQ
                     VFLSLIDAMASSNKAIIHVSQKEAIRQ"
     gene            complement(6658..7818)
                     /gene="albE"
                     /locus_tag="PO770_RS01920"
                     /old_locus_tag="PO770_01925"
     CDS             complement(6658..7818)
                     /codon_start=1
                     /gene="albE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16_C"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391621.1"
                     /locus_tag="PO770_RS01920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_01925"
                     /product="antilisterial bacteriocin subtilosin biosynthesis
                     protein AlbE"
                     /protein_id="WP_128442096.1"
                     /sec_met_domain="Peptidase_M16_C (E-value: 5.4e-06,
                     bitscore: 22.6, seeds: 139, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEVNLLKTHQFSTISIAASFLKPIESAAEPEEETIYFYGAAAYLK
                     EQIIDAFGCAAGSRFMYSANLFFDQQLKTCGTRLIHPLYNGNLHVDALLKTFADLSFPS
                     SLSFEAVEKARNELLLRIEKKFTDPFSYSAARLAEEVFGNPMYGTGMFGRKDRIKAIHP
                     KRFLDATDFIVDLVSQQKQLNILGQVQACDVRGHAPQTSAVTSGRIPVNRHVFETETRS
                     AAGLSVLTLGFDCGEMKDASDYIKIQLIDGLLGKYGHSALFKHFREKDLAVYHVITRYD
                     VMNNLLLVSICTDQLHEKDIPPRVLEAVSAFHTDERELEQAKQFLRNELLLQFDSPEGL
                     LAYMGILRRFSCTKEALLDGISGVTCRDVLQFIATINYIGAHVVRG"
     gene            complement(7808..9118)
                     /gene="albD"
                     /locus_tag="PO770_RS01925"
                     /old_locus_tag="PO770_01930"
     CDS             complement(7808..9118)
                     /codon_start=1
                     /gene="albD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391620.1"
                     /locus_tag="PO770_RS01925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_01930"
                     /product="antilisterial bacteriocin subtilosin biosynthesis
                     protein AlbD"
                     /protein_id="WP_128441924.1"
                     /transl_table=11
                     /translation="MNNIILIMSLLFKQLYSRQGKKDAIRIAAGLVILAVFEIGLIRQA
                     GIDESVLRKTYIILALLLMNTYMVFLSVTSQWKESYMKLSCLLPISSRSFWLAQSVVLF
                     VDTCLRRTLFFFILPLFLFGNGTLSGAQTLFWLGRFSFFTVYSIIFGVVLSNHFVKKKN
                     LMFLLHAAIFACVCLSAALMPAATLPLCAVHMLWAVIIDFPVFLQAPPQQGKMHSFMRR
                     SEFSFYKREWNRFISSKAMLLNYAVMAVFSGFFSFQMMNTGIFNQQVIYIVISALLLIC
                     SPIALLYSIEKNDRMLLITLPIKRKTMFWAKYRFYSGLLAGGFLLVVMIVGFISGRPIS
                     VLTFLQCIELLLAGAFIRLTADEKRPSFSWQTEQQLWSGFSKYRSYLFCLPLFLATLAG
                     TAVSLAVIPIAGLVIVYYLQKQDGGFFDTSKRERLGS"
     gene            complement(9111..9830)
                     /gene="albC"
                     /locus_tag="PO770_RS01930"
                     /old_locus_tag="PO770_01935"
     CDS             complement(9111..9830)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene="albC"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391619.1"
                     /locus_tag="PO770_RS01930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_01935"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_072175631.1"
                     /transl_table=11
                     /translation="MSILDIHDVSVWYERDNVILEQVDLHLEKGAVYGLLGVNGAGKTT
                     LINTLTGVNRNFSGRFTLCGIEAEAGMPQKTSDQLKTHRYFAADYPLLFTEITAKDYVS
                     FVHSLYQKDFSEQQFASLAEAFHFSKYTNRRISELSLGNRQKVVLMTGLLLRAPLFILD
                     EPLVGLDVESIEVFYQKMREYCEAGGTILFSSHLLDVVQRFCDYAAILHNKQIQKVIPI
                     GEETDLRREFFEVIGHE"
     gene            complement(9827..9988)
                     /gene="albB"
                     /locus_tag="PO770_RS01935"
                     /old_locus_tag="PO770_01940"
     CDS             complement(9827..9988)
                     /codon_start=1
                     /gene="albB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391618.1"
                     /locus_tag="PO770_RS01935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_01940"
                     /product="antilisterial bacteriocin subtilosin biosynthesis
                     protein AlbB"
                     /protein_id="WP_003222006.1"
                     /transl_table=11
                     /translation="MSPAQRRILLYILSFIFVIGAVVYFVKSDYLFTLIFIAIAILFGM
                     RARKADSR"
     gene            complement(10001..11347)
                     /gene="albA"
                     /locus_tag="PO770_RS01940"
                     /old_locus_tag="PO770_01945"
     CDS             complement(10001..11347)
                     /codon_start=1
                     /gene="albA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: subtilosin"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Subtilosin_rSAM_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR04085"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) SPASM"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391617.1"
                     /locus_tag="PO770_RS01940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_01945"
                     /product="subtilosin maturase AlbA"
                     /protein_id="WP_015714887.1"
                     /sec_met_domain="subtilosin (E-value: 0.0, bitscore:
                     1083.7, seeds: 6, tool: rule-based-clusters)"
                     /sec_met_domain="Subtilosin_rSAM_RRE (E-value: 1.3e-29,
                     bitscore: 98.4, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 8.5e-08, bitscore: 28.4,
                     seeds: 461, tool: rule-based-clusters)"
                     /sec_met_domain="PTHR43273 (E-value: 2.9e-45, bitscore:
                     150.8, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 9e-07, bitscore: 24.6,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 1.4e-22, bitscore: 76.9,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 1.3e-10, bitscore:
                     37.5, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 4.2e-10, bitscore: 36.0,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFIEQMFPFINESVRVHQLPEGGVLEIDYLRDNVSISDFEYLDLN
                     KTAYELCMRMDGQKTAEQILAEQCAVYDESPEDHKDWYYDMLNMLQNKQVIQLGNRASR
                     HTITTSGSNEFPMPLHATFELTHRCNLKCAHCYLESSPEALGTVSIEQFKKTADMLFDN
                     GVLTCEITGGEIFVHPNANEILDYVCKKFKKVAVLTNGTLMRKESLELLKAYKQKIIVG
                     ISLDSVNSEVHDSFRGRKGSFAQTCKTIKLLSDHGIFVRVAMSVFEKNMWEIHDMAQKV
                     RDLGAKAFSYNWVDDFGRGRDIVHPTKDAEQHRKFMEYEQNVIDEFKDLIPIIPYERKR
                     AANCGAGWKSIVISPFGEVRPCALFPKEFSLGNIFHDSYESIFNSPLVHKLWQAQAPRF
                     SEHCMKDKCPFSGYCGGCYLKGLNSNKYHRKNICSWAKNEQLEDVVQLI"
     aSDomain        complement(11057..11332)
                     /aSDomain="Subtilosin_rSAM_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing rSAM protein in a subtilosin
                     sactipeptide cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_PO770_RS01940_Subtilosin_rSAM_RRE.1"
                     /evalue="3.40E-31"
                     /identifier="RREFam018.1"
                     /label="PO770_RS01940"
                     /locus_tag="PO770_RS01940"
                     /protein_end="97"
                     /protein_start="5"
                     /score="98.4"
                     /tool="antismash"
                     /translation="MFPFINESVRVHQLPEGGVLEIDYLRDNVSISDFEYLDLNKTAYE
                     LCMRMDGQKTAEQILAEQCAVYDESPEDHKDWYYDMLNMLQNKQVIQ"
     gene            complement(11372..11524)
                     /gene="sboX"
                     /locus_tag="PO770_RS01945"
                     /old_locus_tag="PO770_01950"
     CDS             complement(11372..11524)
                     /codon_start=1
                     /gene="sboX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_054594.1"
                     /locus_tag="PO770_RS01945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_01950"
                     /product="bacteriocin-like protein SboX"
                     /protein_id="WP_010886632.1"
                     /transl_table=11
                     /translation="MKLPVQQVYSVYGGKDLPKGHSHSTMPFLSKLQFLTKIYLLDIHT
                     QPFFI"
     gene            complement(11481..11612)
                     /gene="sboA"
                     /locus_tag="PO770_RS01950"
                     /old_locus_tag="PO770_01955"
     CDS             complement(11481..11612)
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /gene="sboA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: Subtilosin_A"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391616.1"
                     /locus_tag="PO770_RS01950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_01955"
                     /product="subtilosin A"
                     /protein_id="WP_003222002.1"
                     /sec_met_domain="Subtilosin_A (E-value: 2.3e-29, bitscore:
                     97.4, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKAVIVENKGCATCSIGAACLVDGPIPDFEIAGATGLFGLWG"
     CDS_motif       complement(11583..11612)
                     /prepeptide="leader"
                     /note="peptide class: sactipeptide"
                     /note="predicted sequence: MKKAVIVENK"
                     /locus_tag="PO770_RS01950_sactipeptide"
                     /aSTool="sactipeptides"
                     /tool="antismash"
     CDS_motif       complement(11481..11582)
                     /prepeptide="core"
                     /locus_tag="PO770_RS01950_sactipeptide"
                     /peptide="sactipeptide"
                     /predicted_class
                     /score="33.00"
                     /molecular_weight="0.0"
                     /monoisotopic_mass="0.0"
                     /core_sequence="GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG"
                     /aSTool="sactipeptides"
                     /tool="antismash"
                     /leader_sequence="MKKAVIVENK"
                     /leader_location="[11582:11612](-)"
     misc_feature    complement(11703..11718)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 25.99"
                     /tool="antismash"
     gene            11925..12353
                     /gene="ywiB"
                     /locus_tag="PO770_RS01955"
                     /old_locus_tag="PO770_01960"
     CDS             11925..12353
                     /codon_start=1
                     /gene="ywiB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391615.1"
                     /locus_tag="PO770_RS01955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_01960"
                     /product="DUF1934 domain-containing protein"
                     /protein_id="WP_003243604.1"
                     /transl_table=11
                     /translation="MKQETPITLHVKSVIEDDGNQEVIEFRTTGFYYVKQNKVYLSYYE
                     EHDLGKVKTIVKVSEGEVLVMRSGAVKMNQRFVTGASTIAKYKMSFGELELKTSTKSIQ
                     SDLDEEKGRISIAYDMHVGDEQEHLHNMTITYEGGTHA"
     gene            12350..14020
                     /gene="argS"
                     /locus_tag="PO770_RS01960"
                     /old_locus_tag="PO770_01965"
     CDS             12350..14020
                     /EC_number="6.1.1.19"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="argS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391614.1"
                     /locus_tag="PO770_RS01960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_01965"
                     /product="arginine--tRNA ligase"
                     /protein_id="WP_003227570.1"
                     /transl_table=11
                     /translation="MNIAEQMKDVLKEEIKAAVLKAGLAEESQIPNVVLETPKDKTHGD
                     YSTNMAMQLARVAKKAPRQIAEEIVAHFDKGKASIEKLDIAGPGFINFYMNNQYLTKLI
                     PSVLEAGEAYGETNIGNGERVQVEFVSANPTGDLHLGHARGAAVGDSLCNVLSKAGYDV
                     SREYYINDAGNQINNLALSVEVRYFEALGLEKPMPEDGYRGEDIIAIGKRLAEEYGDRF
                     VNEEESERLAFFREYGLKYELEKLRKDLENFRVPFDVWYSETSLYQNGKIDKALEALRE
                     KGHVYEEDGATWFRSTTFGDDKDRVLIKKDGTYTYLLPDIAYHKDKLDRGFDKLINVWG
                     ADHHGYIPRMKAAIEALGYEKGTLEVEIIQLVHLYKNGEKMKMSKRTGKAVTMRDLIEE
                     VGLDAVRYFFAMRSADTHMDFDLDLAVSTSNENPVYYAQYAHARICSMLRQGEEQGLKP
                     AADLDFSHIQSEKEYDLLKTIGGFPEAVAEAAEKRIPHRVTNYIYDLASALHSFYNAEK
                     VIDPENEEKSRARLALMKATQITLNNALQLIGVSAPEKM"
     misc_feature    complement(14076..14091)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 19.26"
                     /tool="antismash"
     gene            14156..15343
                     /gene="narK"
                     /locus_tag="PO770_RS01965"
                     /old_locus_tag="PO770_01970"
     CDS             14156..15343
                     /codon_start=1
                     /gene="narK"
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391613.2"
                     /locus_tag="PO770_RS01965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_01970"
                     /product="nitrate transporter NarK"
                     /protein_id="WP_010886631.1"
                     /transl_table=11
                     /translation="MINRQHIQLSLQSLSLVAGFMVWVLISSLISQITLDIHLSKGEIS
                     LVTAIPVILGSLLRIPLGYLTNRFGARLMFMVSFILLLFPVFWISIADSLFDLIAGGFF
                     LGIGGAVFSIGVTSLPKYYPKEKHGVVNGIYGAGNIGTAVTTFAAPVIAQAVGWKSTVQ
                     MYLILLAVFALLHVLFGDRHEKKVKVSVKTQIKAVYRNHVLWFLSLFYFITFGAFVAFT
                     IYLPNFLVEHFGLNPADAGLRTAGFIAVSTLLRPAGGFLADKMSPLRILMFVFAGLTLS
                     GIILSFSPTIGLYTFGSLTVAVCSGIGNGTVFKLVPFYFSKQAGIANGIVSAMGGLGGF
                     FPPLILASVFQATGQYAIGFMALSEVALASFVLVIWMYWQERMKTHTERNSQSIN"
     gene            15442..16158
                     /gene="fnr"
                     /locus_tag="PO770_RS01970"
                     /old_locus_tag="PO770_01975"
     CDS             15442..16158
                     /codon_start=1
                     /gene="fnr"
                     /gene_functions="regulatory (smcogs) SMCOG1133:
                     Transcription regulator, crp"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391612.1"
                     /locus_tag="PO770_RS01970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_01975"
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="WP_014481215.1"
                     /transl_table=11
                     /translation="MNFLSVRPSDSDLISSDLHELLESISTKRKMEKHTYLFREGMDAE
                     ELYLIQSGLIEIGKLTSDGKDLTLRICQKHDIVGELTLFTEEPRYMLSAKVLEDGEVLV
                     INKNKLEKELIQNGALTFEFMKWMSTHLRKIQSKIRDLLLHGKKGALYSTLIRLSNSYG
                     VERSDGILINIVLTNQDLAKFCAAARESINRMLGDLRKKGVISIDESGKIILHKRDYLR
                     CEIECENCPLEICNID"
     gene            complement(16190..16909)
                     /gene="ywiC"
                     /locus_tag="PO770_RS01975"
                     /old_locus_tag="PO770_01980"
     CDS             complement(16190..16909)
                     /codon_start=1
                     /gene="ywiC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391611.1"
                     /locus_tag="PO770_RS01975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_01980"
                     /product="YwiC-like family protein"
                     /protein_id="WP_163138411.1"
                     /transl_table=11
                     /translation="MKALIPKQHGAWAMLLIPFLLGMAMGGPVIWHIPLFLGWLFLYLA
                     VYPVTLALKKKQSKPYQKWMCYYGFPACCFLMISVFHKPPLIWVGVSLLPLFLIHMYFA
                     RRKNERALLNDVAGVLFFCSGGFASCWLGMGTLDGWAWFIFLQSALFFIGSSFYVKSVI
                     RERKNRTFAYWSWGYHLLLPFLSALFGAGWAFLAFIPSSLRAWFFHGRDWPVKTIGILE
                     IVNACFFFAVMCLFMTR"
     misc_feature    16980..16995
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.4"
                     /tool="antismash"
     gene            17053..17529
                     /gene="arfM"
                     /locus_tag="PO770_RS01980"
                     /old_locus_tag="PO770_01985"
     CDS             17053..17529
                     /codon_start=1
                     /gene="arfM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391610.1"
                     /locus_tag="PO770_RS01980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_01985"
                     /product="Crp/Fnr family transcriptional regulator ArfM"
                     /protein_id="WP_064671464.1"
                     /transl_table=11
                     /translation="MNQCDYLLFLKQLPMFNEVPLSIVETLLKNGTFIRGSCDQSPSFL
                     HSQSVYIVLKGSVRFVDSRLPEGSKTVALWEKGDVFPIDEKGGLYLSPFISVNATSDIL
                     ILNIPYYIFKKMMSYHPQLQMNFLAMLQQNVFCSYQLFLRYLHTSQDENAEPGS"
     misc_feature    complement(17621..17635)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 21.64"
                     /tool="antismash"
     misc_feature    17622..17635
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.07"
                     /tool="antismash"
     misc_feature    complement(17644..17659)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 19.26"
                     /tool="antismash"
     gene            17725..21411
                     /gene="narG"
                     /locus_tag="PO770_RS01985"
                     /old_locus_tag="PO770_01990"
     CDS             17725..21411
                     /GO_component="GO:0009325 - nitrate reductase complex
                     [Evidence IEA]"
                     /GO_function="GO:0008940 - nitrate reductase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="narG"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1134: flavodoxin"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391609.2"
                     /locus_tag="PO770_RS01985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PO770_01990"
                     /product="nitrate reductase subunit alpha"
                     /protein_id="WP_161619170.1"
                     /transl_table=11
                     /translation="MKKKKRSPLFRRLNYFSPIEHHSNKHSQTTREDRDWENVYRNRWQ
                     YDKVVRSTHGVNCTGSCSWNIYVKNGIVTWEGQNLNYPSTGPDMPDFEPRGCPRGASFS
                     WYIYSPLRVKYPYVRGVLINLWREALQEHQNPLEAWKSIVENPEKAKSYKQARGKGGFV
                     RAEWPEVLKLISASLLYTVMKYGPDRNVGFSPIPAMSMISHASGSRFMSLIGGPMLSFY
                     DWYADLPPASPQIWGDQTDVPESSDWYNSGYIITWGSNVPLTRTPDAHFLAEARYKGAK
                     VISISPDFAESSKFADDWLSIRQGTDGALAMAMGHVILQEFYVNQETERFIEYAKQYTD
                     FPFLVTLSKENGVYTAGRFLHAKDIGRQTKHDQWKPAVWDEQTSSFAIPQGTMGSRWDG
                     QQKWNLHMIDEETGEPIEPRLSVLGIEDEIGTVRIPYFSNDGNKVLERDLPIKKMNLNG
                     EETYITTVFDLILANYGVNRGIGERSAVSYDDPEPFTPAWQEQMTGIKKEAVVKIAREF
                     AQNAIDTDGRSMIIVGAGINHWFNSDTIYRAVLNLVLLVGAQGVNGGGWAHYVGQEKLR
                     PAEGWQTIATAKDWEGVPKLQNGTSFFYFATDQWRYEDQPISDLASPIAASSRYKHHAD
                     YNVLAARLGWLPSYPTFNQNGIDLYKEAEKAGAETPEDVGAYVASQLQEKKLKFAIEDP
                     DNEVNFPRNLFVWRANLISSSGKGHEYFLKHLLGTTNGLMNDDSDSIRPEEIKWREQAP
                     EGKLDLLINLDFRMAGTALYSDIVLPAATWYEKHDLSSTDMHPFIHPFAPAISAPWESK
                     SDWDIFKALSKAVSDLAEEVDMEPVKEVVATPLLHDTMQELAQPFGKINDWSKGECEAI
                     PGKTMPNIQVVERDYKHIFHKMTALGPNVALKPSGTKGMSWSIADEYESLKQRLGEITS
                     DSVAKGCPNISEAKQAAEAILTLSSTSNGKVAVKAWESLENITNLKLKDLAEEREEECF
                     TFEQITAQPKTVITSPAFSGSEKGGRRYSPFTTNVEKLIPWRTLTGRQSYYVDHELMME
                     FGETMATFKPILQHRPFLSKRPDQEGKEIVLNYLTPHNKWSVHSMYFDSLPMLTLFRGG
                     PTVWMNKDDAEDTDIKDNDWIECFNRNGVVVARAVLSHRIPKGMAFMHHAQDRHINVPG
                     TKLTNNRGGTHNSPTRIHVKPTQMIGGYAQLSYGFNYYGPTGNQRDLNVVIRKLKEVDW
                     LED"
     misc_feature    21489..21503
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 20.21"
                     /tool="antismash"
ORIGIN
        1 ggatcatgag tctatccaat tgggcaaact cctccaattt catggcctgt ctgctccagt
       61 acctcattag gagagagcat tcttgcttta aagccaaatg tttgcctgga ctttttgttg
      121 tcgatcttgg catcgccggc tgccacaatc agaatcacct gatctccttc ccctctgaag
      181 gacagggact tggcgattct agacaggctt acgccgattg tctcagctgc ctgttcaact
      241 gtcgcactcg acgtttcgaa ctccgtcaca tcattttctc gattccattg agtaaagtgg
      301 gttctcacac tttttagaga catgctgcat ttccactatt tttaagatta ttgaaaaaat
      361 catagaatgg attgggagac tgggtaaaga ggggacaaaa aaaccgccga tggacggcgg
      421 ttaaatcatt cctcgttgtg caacttcaat ttggtgtgta ggtgcatttg caatagttgt
      481 tgccacgaac acagtgctta gagccagaca agagagtaat agtttagact tcaatttcat
      541 acaaactcac tcctccttga ataagttgcc ttacttgttc caccttcaaa aaataagcag
      601 aagctttttg aaaattttta cgttcatgat aatattttgc cacatcaata gcgaaatctt
      661 caagatcagc atacaacatt ttactttcga gaaaattaaa aaatccttga attgcttctt
      721 catccgggcc tgataagtat aaagatttca aaaattcaaa ctctgataaa tatattacat
      781 ctccggcctt ttgtgaataa gccattccct tactatgata ttcatgagct ttatccattt
      841 ttcctaattt ataatggatc tgtgtaatta aaaaatacgc ttgaggtaag gaaggaagaa
      901 tatttgattc ttcaaaaaca gctattgctc ttttgaaata aggtatggca tcctcatatt
      961 ggctttggct gtttttacaa agcccgatat tgtacaaagt tcttcccatt aattggggct
     1021 gcttttcagc ttctgccata gaataagctt tttgaaaatg tgagatggca tcctcatact
     1081 gttttaaatc taaaaaattg gtggcaaata aagaatgaca ctgcagcaat cttatattat
     1141 aagcttcatt ttctttgtat atttcatatg cttgccgtgc atagtccatt gaaaaatacg
     1201 tttgtttcat atagtaataa gattcagaca tcttaaagaa aaactcagct ttctctatcc
     1261 gatccttaac gaatatcagc ttgctttcgg cctttttgaa aaatttaata gccgacagat
     1321 attcccgctg gtccagctca tacatgcctc tgaagaaatt aaaatagtac tcaagcagac
     1381 cagttaaacg agcctgtttt ttatcaatct caagcagcag atcagacagt ctcggctgtt
     1441 cctcaatcct cattttttct aacggttcaa ggtactcaag cattaggttg tgccgaaact
     1501 ccatcagtga atagtacaaa tgaaggtctt gatcttcttc catttgatcc agctcttgct
     1561 tgatttctcg tcgcaaatat tctgcatcgg gtatgctgaa tcggcgtatg tacatatacc
     1621 attcgttaat cttttctccg atggaagaag aagatatgac acctgtcacg tttgcaaatc
     1681 cctcccttca taattctata tatgtcatat tttatcatca acattcttgt gagaagcgca
     1741 aaaaaaccgc aattttttct ttcgttacac cgcatgtttg gagatatatt ttcaaaaaga
     1801 agcgtttacc ctcagtaatt tgtacaagca aacacgttga tcttccatat tctgttatat
     1861 ctgcgtgagc agtgtatgtc tgaaaaggaa gtgaaggaat gagaaaaaat aagctttcct
     1921 ttaaagaaaa agcaggagca gaacaagaag agtgcttatg tttttgtgag ggagaagcaa
     1981 gccgagaagc catgtttcaa gcgccgattg aattacctga aggatttgtc gttgatccgg
     2041 ctgaagctgt tgcaaacgtg acgtggaatg cagacagcct ttcttgtgtc agtgaccgtt
     2101 gcttaattca aacggggcca gagcctgatg aggtcggtgt tcaatttgca gtgcgcctgc
     2161 agggaaccgt tacgctactc gtcagtgtct cacctgtgcg aaatcaatat ggacaagggg
     2221 acggggctgt ttccgtcatt cacaatgcag aaatcgatca agtcgtctat tattccgatg
     2281 aaccggatga ttgcccggat atcagtgaca tcacgattga gaaccttgtt gttgttccgc
     2341 cgttttacgg cagtccttta tcggtgacgg ggacgattgt gcttgtaccg gagccggaac
     2401 ccgtctacgc gtttacagcg aatccaaatg atcaatcagt ttctgtgatt gatacaaaca
     2461 cccatactgt tgtgacaacg attgctcttc cgtacaatcc ggcaggtatt gaaattacgc
     2521 cagataaaag cgctgtgttt gtcttacatc ccaacaataa tgtgatttct gtcatcgatt
     2581 atgacacatt aacagtgaca gcaactatat tgctggatca gccgcctcga ttgattagat
     2641 ttatccctaa tcatgagttt gcttatgttt tcaccggtac tgccatttat gtgattggaa
     2701 ttgatacgtt gactgttgag aggtctattc ctgtagaagg ttttgatatt gcaattgacc
     2761 ccaatgggtt gttcgcctat gttcttaatt ttgggttagt gcaaaaagtg gacttatcta
     2821 ccggtgaagt cttaggaacg attgaacgag agcttatcgt atcatccata gaaacagatt
     2881 ctacggagcg gtatgcgtat gtattagaac aagaattctt attcaattat ttgacggtta
     2941 ttgatttaaa tacgtttacg atcagggata cccaagagct ggagcctgac gggctatatg
     3001 taatgtttac gagtggatca gaggtgtatt tacatgacag cttcactggc aatttatatt
     3061 ctgtcagtcc gaatggagca ggtgtaattg gaaatcttcc gcaatcagca acagactatg
     3121 cgtttacctc gaatggcgat tttctgtatg caactcgttt tatagagcag agcatcattg
     3181 tttacaacac agatgattat tctgaggaaa ctgtgatatc tcttagggtt tcaccgggtg
     3241 ccattacgat ttaaccgctg catgttgtgc atttctaaaa ggacgtgaaa tcatgaaaaa
     3301 gaatgatctt tctgctttac aggaaaattg tttctgtttt tgtgatgagg aaattagccg
     3361 ggaagcgccg tttcaagtgc cgatagatat ccctgagggc ttcaaggttg attcggcaga
     3421 agcgtcagcg gctgttacat ggagtacaga caatttgtcc tgtatcagtg agccttgctt
     3481 aatccagact gggccggaac cagaagatat cggtgttcga tatgccgtga gagtacaagg
     3541 gaccattacg cttcttgtca gtgtgtcgcc tgtacgaaat caatatggac aaggagacgg
     3601 ggctgtttcg gtgattcata ccgaagagat tgatcaagtt gtttattatg cagcacagtc
     3661 tgaccgctgt cctgatttta gtcaaatgac agtggaagat ctgctcattg taccgccttt
     3721 ttacggcagt ccgcttacgg tggccgggac acttgtttta ccgccatcac cagatcctca
     3781 atcgtatgtg tttactgcca atactggaga cagcacggtt tcggttattg atgcagattt
     3841 aaacactgtt gtaaaaacaa tccctttttc tgacgtgcca actaatctag gcgttacttt
     3901 cgataaagca ttcacgtatg tcctccatgg aaataccaac cttgtctcag tgattgataa
     3961 taaaacatta accatcataa ataccattac tgttggagga gggccgagaa agatcgaatt
     4021 tgatccaaca gatgagttcg catacgtgat ggccgccgga tctatatatg tgattaacac
     4081 ggcctctcaa tcggtcattg atgtcattcc gatacccggc gctttagact ttgctcttga
     4141 tccgaatgga cagtatgtct ataccgctaa tggaagcagt tggtcggttg ataaatatga
     4201 tgtaaatact gggcagttgg tagaaagtat catagattca tttgaatttc ccagtttaat
     4261 tgcaactcct tacgctggaa attttgcgta cgtgttgaat ggtgagctgt ggccgaaagg
     4321 tgtaacggag attagtttgt ctccattaag caggggaggg gacttcagcc gtttatttga
     4381 aacgctgagg acgattgtat tttctctcga tagtactaga gcttattttc tagagccata
     4441 tagtgaaccg tttctcatta ataacttata tgtagttaat acggccagac agaggatcat
     4501 tgcaaacgtg tctctccccg gcgcctttga cctcgccgta acgccggaca aacagtatat
     4561 ttatgcggca cagcccaacg acaatgctgt gaccgtgtac cgcacaagtg attatacagc
     4621 cgtgacggtg attccggttg gcgcgggtcc gtctgcgatt gcgatgtagc tattttatag
     4681 atcaagagaa gcggtgctgc gataagagaa tcgtttataa ataaatgatc cgatttgctg
     4741 aatggcagcc aggataatca gaaacagcag ttcacttata gagaaactga gcatccacca
     4801 aatcccggct gctgccagca ctcccgcaaa cacttttctg aacaggctgc cgtgccggaa
     4861 aagctgccgc aaataagttc gcatgcaaaa caataaatac aggatcagta gcatcgcaag
     4921 tcccattgaa agggcagggg gcgtgtgaag gcggaaaggg acggctttaa cgatttcgaa
     4981 catgcagccg aatatgatga cttggcacaa cacggcagcc atcacgtatg tttggcggac
     5041 gtttttatag ctgtcacgca gcgatttgac gtcttctgca tatcggctgc acaagttgtg
     5101 ttgctgaaag tcacagcagc tttgatgaga ctgtgtgagt ttctgaaaag ctgctgtcat
     5161 gtttttcacg gattgggtga cataatgaag atagaaaaaa gcaggcagca tcaggagggc
     5221 agcggtacaa gccagcagcc cgagccgcac agcgtcattg ttgatcatct gtacatattg
     5281 ggcggcggct tcggccgcgg cctgaaccca gccgaaggaa taagccgcca tacctataag
     5341 cagcaataaa agggtaaaga cagtgttttt tttcattgtc tgatcgcctc tttctgagaa
     5401 acgtgaatga tcgctttgtt agaagatgcc attgcatcaa tgagagacaa gaaaacctgc
     5461 gcatccatat cttgaatgat gtggtacatg tcaagaaacg agcaatcgca cctgtcacac
     5521 tcggctgccc actcgaagca tttacgcaca taatcgctgt cataaatgat cgcgtctgtc
     5581 aaagagtcct tgccgtattg aagccaggcg gcaacctgtt ctttcttctg tgtgacatag
     5641 tatgaagcca catcgatata agctgaagaa tgggtccctt gactgtgaag gatcaagaaa
     5701 ccgccatcct catatatata agtctcggct gacaagcgat cgatttccag acggtacgtt
     5761 tgctgaatct cttgaaaaaa cggtgaatct aattgaaata gaatacgagc cgcgatatcc
     5821 caataaagct ttatcgacaa cagatcgttt tgcccgggaa gtgcgccgat ctgtaatccg
     5881 gtccaagtat cttccatccg ctcctcatca cccagcacca ttttttgtga gagggctgga
     5941 gggcatgccg ggatgatttt tctttcagca tgataatccg gacgcttttc cagctggagg
     6001 tgaggaggca aaagcgtttc tgtattttct gagcccccaa tcaaaaacag tgtcatccga
     6061 tgggcttggt aggcagcctt ataggctttt tgtaaatcat tgatgtccag cgcctcaata
     6121 tcttcgattc tgcctgcggg gaaaacggca gctggtgagg agggagaaag catgctgatc
     6181 agctgataat gatatgagag ctgatggtta aggtgtgctg tttgaatttc gcttgtaatc
     6241 acagcttttt cctgagcgac aatcttcttg tcaaatgagt ggttccataa agcatcaagc
     6301 aggatgggga tcgtttgtct gagccgatcc ggtaatgagg taaacatgaa attggtatcg
     6361 gtgtaggtag tgaatgcgtt taaaagcgcg ccatgcgcaa aaaaatcact gtacagattg
     6421 cggccgttat gccagaagag aagatgctct aaaaaatgtg ctgtgccata cggaagcagt
     6481 gtatcaccgg attctgcccg tatacataca tcccggcttc caaaatcgat ttttacattg
     6541 cacagccgca ggtgggcgcg ggggaatttc aatctgaata tttttagtcc gctgtctgtg
     6601 tatctgatat cggttcgttc atccagctgt tgaaaaaagg ctttcttttc catctcttca
     6661 tccccttact acatgagctc cgatataatt gatggtcgct atgaattgca atacatcacg
     6721 gcatgtaacc cctgagatgc catccagcaa ggcttctttc gtgcaggaga agcggcgcag
     6781 gatccccata taggccaata gaccttcggg agaatcgaat tgcagcagca attcattgcg
     6841 taaaaactgc tttgcctgtt ctagttcccg ctcatctgta tgaaaagctg agacagcttc
     6901 caaaacgcgc gggggaatgt ctttctcatg aagctgatct gtacatattg aaacaagcag
     6961 caaattgttc atgacatcgt aacgggtgat aacgtgatag actgccagat ccttctctct
     7021 gaagtgcttg aataaagcgg aatggccgta ctttcccagt aatccgtcta ttagctgtat
     7081 tttgatataa tcgctggcat ctttcatctc tccgcagtca aatccgagtg tcaagacgga
     7141 aagacctgca gcgctgcgtg tctctgtttc aaaaacatgc cggtttacgg gtattcttcc
     7201 ggacgttaca gcggacgttt gcggggcatg tccccgaaca tcacatgctt gtacttgtcc
     7261 caatatgttc agctgttttt gctgggacac taaatcaacg ataaagtccg ttgcgtcgag
     7321 aaaccgttta ggatgaatcg cctttatcct gtctttccgg ccaaacatgc ctgtgccgta
     7381 catcgggttt ccgaagacct cctcagccaa gcgcgccgcc gagtaggaga aaggatcagt
     7441 gaatttcttt tcgattctca gcagcagctc attcctggcc ttttcaactg cttcgaatga
     7501 aagagaagaa gggaaagaca agtctgcaaa agttttcagc agggcgtcaa catgaagatt
     7561 cccgttatat aatgggtgaa tcaaacgggt cccgcaggtt ttcagctgct gatcaaaaaa
     7621 gagattcgct gagtacatga aacggctgcc ggcagcacaa ccgaaagcgt caatgatttg
     7681 ctccttcagg tatgcggcgg cgccgtaaaa ataaattgtt tcttcctcag gttccgccgc
     7741 gctttcgatt ggtttcagaa agctcgcggc gattgaaata gttgaaaatt gatgagtctt
     7801 cagcaggtta acttccaagc cgttctcttt tgcttgtatc aaagaaacca ccatcctgtt
     7861 tttgcaggta atagactatg accagaccgg ctatcggaat gaccgccaag gatacagcgg
     7921 tgccggctaa tgtggctaag aataggggaa gacaaaacag ataagaccga tattttgaga
     7981 aaccgctcca tagctgctgc tccgtctgcc agctgaagga gggccttttt tcatccgcag
     8041 ttaacctgat aaaagctccc gccagcagca actcaatgca ctgcaaaaaa gtgagaactg
     8101 aaatcggacg gccgctgatg aaacccacaa tcatcacgac aaggagaaat ccgcctgcca
     8161 ataggcctga ataaaagcga tatttcgccc aaaacatcgt tttccgcttg atcggaagcg
     8221 tgatgagcaa catccggtca tttttttcaa tcgagtacaa aagggcgatc ggcgagcaga
     8281 tgagcaaaag cgcggaaatc acgatataaa tcacttgctg attgaagatg ccggtgttca
     8341 tcatctggaa cgaaaagaag ccgctgaata ccgccattac cgcgtaattt aacagcatcg
     8401 ctttagaaga gataaatcgg ttccattctc ttttgtaaaa cgaaaattca gatcgccgca
     8461 taaatgaatg catcttgccc tgctgcggag gcgcctgcag aaagacagga aagtcaatga
     8521 tcaccgccca cagcatatgc accgcgcaaa gcggaagcgt ggcggccggc atcaaagcag
     8581 cgctgagaca tacacaggcg aatatcgccg catgcagcag aaacatcaag ttcttctttt
     8641 tgacgaagtg gttgcttagc acaactccga aaataatgga gtaaacggta aaaaacgaaa
     8701 acctgccgag ccaaaacaat gtttgcgccc ctgacagcgt tccgtttcca aataagaaca
     8761 gcggtaaaat aaaaaagaat aaagttcttc tcaaacaggt atcgacaaac aaaacgacac
     8821 tctgggcgag ccaaaagctc cgtgaagaaa tcggcagcag gcagctcagt ttcatataag
     8881 attccttcca ttgagatgtc acggaaagaa acaccatata tgtgttcatc aaaagaagcg
     8941 cgagtatgat atacgttttg cgcaacaccg attcatcaat gccggcctgg cggatcagcc
     9001 cgatttcaaa cacggccaga atgacaaggc ctgcggcaat gcggatggcg tcctttttcc
     9061 cttgccggct gtaaagctgt ttgaacagca aagacatgat gaggattatg ttattcatgg
     9121 ccgatcacct cgaaaaattc ccgccgcaga tcggtctcct ctccaatcgg aatgaccttt
     9181 tggatctgtt tgttgtgaag aatagccgca taatcacaaa atctttgcac gacatcgagc
     9241 agatgagaag aaaacaaaat ggtcccgcct gcttcacagt actcgcgcat tttctgataa
     9301 aaaacctcta tcgattccac atccaaaccg acgagcggct catccaaaat aaacagggga
     9361 gcgcgcagca ataagcctgt catcaataca accttttgcc tgttccccaa ggacagctcc
     9421 gaaattctcc tgttggtgta tttcgaaaaa tgaaaggcct cggccaaact ggcaaactgc
     9481 tgctctgaaa aatccttttg ataaagcgaa tggacgaaag acacatagtc cttcgccgta
     9541 atttctgtaa atagcagcgg gtaatcggcg gcgaagtaac ggtgagtctt cagctgatct
     9601 gatgttttct gcggcatgcc ggcctccgct tcaattccgc acagtgtaaa gcgcccgctg
     9661 aaattgcggt tcacgcctgt cagcgtatta atcagtgtcg tttttccggc gccgtttacc
     9721 ccaagcaacc cgtaaacggc gcctttttct aaatgtaaat ccacttgttc taagatgacg
     9781 ttgtcccgtt cataccaaac ggatacatcg tgtatatcca aaatgctcat cgcgagtcag
     9841 ccttccgcgc gcgcattccg aacagaatgg caatggcaat gaaaatcagc gtaaacagat
     9901 aatcgctttt gacaaaatag acgactgcgc cgatgacaaa gataaatgaa aggatataca
     9961 gtaaaattct tctttgtgct ggtgacaaat cgtttccctc ctaaataagc tggaccacgt
    10021 cttctaattg ttcatttttc gcccaagagc aaatgttttt tcggtgatat ttgttagagt
    10081 tcagcccttt taagtaacag cctccgcaat agccgctgaa cgggcatttg tctttcatgc
    10141 aatgttcgct gaaccgcggc gcttgcgctt gccacagttt atggacgaga ggggagttaa
    10201 agatgctttc ataggaatca tgaaaaatat ttcccaatga aaattccttt ggaaagaggg
    10261 cgcaaggacg tacttcgccg aacgggctga tcacaatgga cttccagcca gcgccgcaat
    10321 ttgccgcgcg ttttctctca taaggaataa tcggaatcag atctttaaac tcatcaatca
    10381 cattttgctc gtattccata aacttgcggt gctgctcggc gtctttcgtt ggatggacaa
    10441 tatccctgcc tcttccgaaa tcgtcaaccc aattgtaaga gaacgccttc gccccgagat
    10501 cccgaacctt ttgggccata tcgtggattt cccacatgtt tttttcgaag acagacatgg
    10561 cgactctgac aaatataccg tggtcactca acaattttat cgttttacaa gtttgggcaa
    10621 aagagccttt tctccctcta aaggagtcat ggacctcaga attgacacta tcaagagaaa
    10681 tgccgacgat gattttttgc ttgtaagcct tcaaaagctc caaactctct tttcgcatga
    10741 gtgttccgtt tgttaagaca gcgacttttt tgaacttttt acacacatag tcaagaatct
    10801 cattggcgtt tggatggaca aaaatttctc cgcctgtgat ttcgcatgtc aatacaccgt
    10861 tatcaaacag catatcagcc gtttttttga attgctcaat cgacacggtg ccgagcgctt
    10921 caggtgagct ttccaaataa cagtgggcgc atttcaaatt acagcggtgc gtcagttcaa
    10981 aggtggcgtg gagcggcatc ggaaattcat tgcttccgct cgtggtgatt gtatggcggc
    11041 tggcccggtt tccaagctga ataacctgct tgttctggag catgttgagc atgtcgtaat
    11101 accaatcttt atggtcttcc ggtgattcat catacactgc acattgctca gctaaaatct
    11161 gctcagctgt tttttggcca tccatgcgca tgcagagctc gtaagccgtt ttgttgagat
    11221 ccaaatactc aaagtcagaa atggagacat tatcgcgcaa gtagtcgatt tctaacacgc
    11281 cgccctcagg aagctggtga actcttacac tttcattaat aaatggaaac atctgctcta
    11341 taaacaaaat accatcccct atacatatga ttcaaatgaa aaacggttgt gtatgtatat
    11401 ccaagaggta gattttagtt aaaaattgta atttacttaa aaagggcata gtagaatgac
    11461 tatgcccttt tgggagatcc ttacccccat agaccgaata gacctgttgc accggcaatt
    11521 tcaaaatcag ggataggacc gtccactaga caagcggctc cgatcgagca tgttgcacaa
    11581 cctttgtttt ctacaatgac agcttttttc ataattgaat cctccctttt tttgtttttc
    11641 tagcagatct atgttgtact tttatcttaa aactatcaaa ttactaattc aatacatata
    11701 atttgaaaaa aatgtgaaaa gaaaatgata gatttcggaa aaatgtaaca tttcctaaag
    11761 gtaattagtg atatgatttt ctcttctata taaggaaatg ctttttatga ggtttatctt
    11821 caaatctgat gagcaatgat ggtaagatag aagcagttga agtttacaaa ccgaatgatt
    11881 atactattca agtcaaagtg gaatgatgaa aaggacgtgt cagaatgaag caagagacac
    11941 cgataacact ccatgtgaag tctgtgatcg aggatgacgg aaaccaagaa gtcatcgaat
    12001 tccgcaccac cggattttac tatgtaaaac aaaataaagt gtatctatct tattatgaag
    12061 aacacgactt aggcaaagtc aaaacgattg tgaaagtaag cgaaggcgaa gtcctggtca
    12121 tgaggtcagg cgctgtcaaa atgaatcagc gtttcgtaac gggagcttcc acaattgcta
    12181 aatataaaat gtcatttggt gagcttgagc tgaaaacaag cacaaaatcg attcaatctg
    12241 atctcgatga agaaaaaggc cgtatcagca tcgcatatga catgcatgtc ggagacgagc
    12301 aggagcattt acataatatg acgatcacgt atgagggagg aactcacgca tgaacattgc
    12361 ggaacaaatg aaggacgtgc tgaaagaaga aatcaaagcg gccgttctga aagcgggact
    12421 ggctgaagaa agccagattc ccaatgttgt tttagaaaca ccgaaagata aaacacacgg
    12481 cgactactca acgaatatgg cgatgcagct ggcaagagtg gcaaaaaaag cgccgcgtca
    12541 aatcgctgaa gagattgtcg cccattttga taaagggaag gcttcgattg aaaaactgga
    12601 catcgccggc ccgggtttta tcaatttcta tatgaacaat cagtatttaa caaagctgat
    12661 tccgtctgta ctggaagcag gggaagcata cggggaaacg aacatcggaa acggtgaacg
    12721 agttcaggta gaattcgtat cagcgaatcc gacgggagac cttcacctcg ggcatgcgcg
    12781 cggggcggct gtcggcgatt ccttgtgcaa cgtgctttca aaagcgggtt acgatgtaag
    12841 ccgcgaatac tacattaacg atgcgggaaa ccaaatcaac aatctggcgc tttctgttga
    12901 agtccgttac tttgaagcac tcggattgga aaaaccgatg ccggaggatg gctaccgcgg
    12961 cgaggatatc attgcgatcg gaaagcgtct cgctgaggaa tacggtgatc gtttcgttaa
    13021 cgaagaggaa agtgaacgcc tcgcgttttt ccgtgaatac ggcctgaaat acgagctgga
    13081 aaagcttcgc aaggacttgg aaaacttccg tgtgccgttt gatgtgtggt attccgaaac
    13141 ttcactgtac caaaatggaa aaatcgacaa ggcgcttgaa gcgctccgcg aaaaaggcca
    13201 tgtctatgaa gaagacggcg cgacttggtt ccgttccacg acgttcggcg atgataaaga
    13261 ccgcgtattg atcaagaagg acggcacgta cacgtacctg cttcctgata tcgcgtatca
    13321 taaggacaag cttgaccgcg gctttgataa gctgatcaac gtttggggcg ccgatcacca
    13381 cggctacatc ccgcgtatga aagcggcaat tgaagcgctg ggttatgaaa aaggaacgct
    13441 tgaagtagaa atcattcagc tcgttcacct ttacaaaaac ggcgagaaaa tgaaaatgag
    13501 caaacggacc ggtaaagccg taacgatgcg tgacctgatt gaagaagtcg gcttggatgc
    13561 cgttcgttac ttctttgcga tgcgaagtgc ggacacccat atggattttg acttagatct
    13621 tgctgtatca acatctaacg aaaaccccgt gtattatgca caatacgcgc atgcacgtat
    13681 ttgcagcatg cttcgccaag gggaagagca gggacttaaa ccggcggcag atcttgattt
    13741 cagccatatt caatcagaaa aagaatacga tctgctgaaa acgatcggcg gcttcccaga
    13801 ggcagtggcg gaagcagcgg aaaaacgaat tccgcaccgt gtcactaact atatttatga
    13861 tctggcttct gctctgcaca gcttctacaa cgcagagaaa gtcatcgacc ctgaaaatga
    13921 agagaaaagc cgcgcgcgcc tggctttaat gaaggcaacg caaattacgc tgaacaatgc
    13981 gcttcagctg atcggcgtat cggctccaga aaaaatgtaa tcacgatcaa aaggacaaag
    14041 tcttcgggct ttgtcctttt tttatgagaa aaacgtgtga tgtaattcac aatcctgttt
    14101 gccgagtttt tgtatgataa gactgattat tgaatcattg aaaggggcaa gccaaatgat
    14161 caaccgtcaa cacattcaat tatcgttaca gtcattaagc ttggtggcag ggtttatggt
    14221 ttgggtgctg atttcatcac tcatttccca aatcacatta gatattcatt taagcaaggg
    14281 cgagatttct ttggtgacgg cgattcccgt tatcctcggg tcgcttctcc gcattccttt
    14341 agggtattta acgaacaggt ttggcgcgag gctcatgttt atggtcagct tcatcctgct
    14401 tttatttcct gtattttgga tcagtatcgc ggactctctg tttgatttaa tcgcaggcgg
    14461 ctttttctta gggatcgggg gagcggtgtt ttccattgga gtgacctccc tcccgaaata
    14521 ttatccgaaa gaaaagcacg gtgtcgtcaa tgggatttac ggtgcaggaa atatcgggac
    14581 agccgttacg acatttgcag cgccggttat cgctcaagcc gtcgggtgga aatcaaccgt
    14641 gcagatgtat ctgattttgc tggctgtctt tgccttactg cacgtattgt ttggcgacag
    14701 gcatgagaag aaggtcaaag tttctgttaa gactcaaatc aaagccgttt accgaaatca
    14761 tgtgctatgg tttctgagcc tgttttattt tattacgttc ggtgcctttg tcgccttcac
    14821 tatctatctg ccgaactttc tggtggagca ttttgggctg aatcctgcag atgccggctt
    14881 gcggacggct ggctttattg cggtttctac gctgctcagg cctgcgggcg gattcctggc
    14941 tgataaaatg agcccgctcc gcatcttgat gtttgttttt gccgggctta cattatcagg
    15001 cattatccta tcgttctcgc cgacaatcgg cctttatacg tttggatcat taaccgtggc
    15061 cgtctgctca ggcataggaa acgggactgt ttttaaactg gtgcctttct atttttcgaa
    15121 gcaagcgggg attgccaatg ggattgtgtc tgccatgggc ggattaggcg gctttttccc
    15181 tccgctcata ttggcgagtg tgtttcaggc aacaggccag tacgcgatcg gatttatggc
    15241 gctttcagag gtggcgttag ccagctttgt ccttgtcata tggatgtatt ggcaggaaag
    15301 aatgaaaaca cacacggaga gaaacagcca gagcatcaac taacactggg gcattcacaa
    15361 gattgttagt tttttctcga tgggtacaaa tcaattcacg ttacaataaa cacacaacgt
    15421 gactttcttc gaggtgagct catgaatttt ctctctgttc gaccatctga tagtgatctt
    15481 atttccagcg atctgcatga attgctggaa tcgatcagca cgaaaagaaa aatggaaaaa
    15541 cacacgtatt tgtttcgaga aggaatggat gcagaagagc tttatctgat tcaatcagga
    15601 ctcattgaaa tcggcaagct gacatctgat ggaaaagacc tgacgcttag aatctgccaa
    15661 aagcatgata tcgtcggaga attaacactt ttcactgaag aaccaaggta tatgctcagt
    15721 gcaaaagtgc tagaggatgg cgaggtgctc gtcattaata aaaataagct ggaaaaagaa
    15781 ttaatccaaa acggcgcttt gacatttgag ttcatgaaat ggatgagcac gcacctccgg
    15841 aagattcagt ccaaaatcag ggatctgctt cttcatggca aaaagggggc gctctattct
    15901 acccttatcc gcttatcaaa cagctacgga gtggagcgaa gcgacggcat tctgattaac
    15961 attgtcctga caaatcagga tttggcaaag ttttgtgcgg ctgcacggga aagcatcaac
    16021 cgtatgctgg gagatctccg caaaaaaggg gttatttcga tagacgaatc cggaaaaatt
    16081 attttacaca agcgggacta tttacgatgt gagattgagt gtgaaaattg cccgctggag
    16141 atttgcaata ttgactgaaa aaaacaagga cagcggtgtc cttgtttttt taccttgtca
    16201 taaagaggca catgacggca aaaaagaagc aggcattcac gatttccaga atgccaatcg
    16261 tttttacggg ccaatccctt ccatgaaaaa accatgcccg caagctgctc gggataaaag
    16321 cgagaaacgc ccatcctgct ccgaaaagag cggataaaaa ggggagcagc aggtgatatc
    16381 cccatgacca gtatgcaaat gtcctgtttt ttctttcacg aatcaccgat ttcacataaa
    16441 aggaactccc gataaaaaat aaagcagact gcaagaaaat aaaccaagcc cagccgtcga
    16501 gggttcccat ccccagccag cagctcgcaa acccgcccga acaaaagaac aagacacctg
    16561 caacatcatt caatagggct cgttcgtttt ttctgcgggc aaaatacata tgtatgagaa
    16621 acagcggcag taaagaaacg cccacccaga tgagaggcgg cttatgaaag accgagatca
    16681 tcaaaaagca gcaggcggga aaaccgtaat aacacatcca cttttgatat ggtttgcttt
    16741 gtttcttttt taaggcaagc gtaacgggat aaaccgctaa gtataggaaa agccagccta
    16801 ggaagagcgg aatatgccaa ataacggggc ctcccatagc catgccgagt aaaaatggga
    16861 tcagcagcat cgcccacgcg ccgtgctgct tcggaatcag cgccttcatc gaaatcctcc
    16921 ttttttcttt cattataaat gcagtcaccc tcctgataag tgacattcat catacaggct
    16981 gtgaaataca tcactgctgt acaggctcag tcgagctaca ctaaagctat ccttagcaca
    17041 ggaggttttc ctatgaatca gtgtgactat ttattatttc tgaaacaact tcctatgttt
    17101 aatgaagtgc ctctatccat tgtcgaaacc ttgctgaaaa acggcacgtt tatccgcggc
    17161 tcatgtgatc agtctccatc ttttcttcat tctcaatctg tttatattgt attaaaaggc
    17221 agtgtccgtt tcgtggacag caggctccct gaagggtcga aaaccgttgc gctgtgggaa
    17281 aaaggcgatg tgtttccgat tgatgaaaaa ggcgggcttt atttatcgcc ctttatctca
    17341 gtcaatgcca cttcagacat tctgattctc aatatcccat attacatatt taagaaaatg
    17401 atgtcttacc atccgcagct gcaaatgaac tttttggcta tgcttcagca aaatgttttt
    17461 tgttcctatc aattattttt gcggtatctg catacatcac aggatgaaaa cgctgagccc
    17521 ggttcttaaa tagagccggt tttttttgat ctttgaatga aaggcggcta atgacccaag
    17581 cgtcctgcct gaacgcagcc ttccttctcc tttgtgtgaa cattttgtga aaaaagcaga
    17641 gtgtgtgaca tagttcacaa ggaaacacgc tgtctctgtt tatagtgaac ttagcatcac
    17701 ctgactcgga aggagtgaac tctcatgaag aaaaagaaaa ggagtccgtt gtttaggaga
    17761 ttgaattatt tctctcctat cgaacaccat tcaaataaac atagccaaac cacccgcgaa
    17821 gatcgcgatt gggagaatgt atacagaaac agatggcagt acgacaaagt cgttcgctcc
    17881 acccacggcg ttaactgtac agggtcttgc agctggaata tttatgtaaa aaacggaatt
    17941 gtcacgtggg aagggcaaaa tttgaattat ccatcaacag gaccggatat gcctgatttt
    18001 gaaccgagag gctgcccgcg gggggccagt ttttcatggt atatctacag cccgctgcgt
    18061 gtgaaatatc catacgtgcg cggtgtactg attaatttat ggcgggaggc actgcaagaa
    18121 catcaaaatc cattggaagc ctggaaatcg atcgtcgaaa accctgaaaa agcgaagtcc
    18181 tataaacagg cgagagggaa aggcggtttt gtgcgcgctg aatggccgga ggtgctgaag
    18241 ctgatttcag cctctctgct gtatacagtg atgaaatatg ggcctgaccg aaacgtcggt
    18301 ttttctccga ttccggccat gtccatgatc agccatgcat caggctcccg gtttatgtcg
    18361 ttaatcggag gccctatgct cagtttttat gactggtatg cggatcttcc accagcatcc
    18421 ccgcaaattt ggggtgacca gacggacgtt ccggaaagca gtgattggta caattccggc
    18481 tatatcatca catggggctc caacgttccg ttaacgagaa cgcctgacgc gcattttttg
    18541 gcggaggccc gctataaagg cgctaaggtc atttcgatca gtccggattt tgcggaatcc
    18601 tcaaagttcg cggatgactg gctgagtatt cgccaaggga ctgacggggc acttgcgatg
    18661 gcgatgggtc atgtcattct gcaggaattt tacgtgaacc aagaaactga acgttttatt
    18721 gagtacgcga agcaatacac tgattttcca tttctcgtca ccctgtcaaa agaaaatggc
    18781 gtatacacag cgggacggtt tctgcatgcg aaggacatcg ggcggcagac aaagcatgat
    18841 cagtggaagc ctgcggtttg ggatgaacaa acaagttcat ttgccatacc ccaagggaca
    18901 atgggatcgc gctgggacgg gcagcagaaa tggaacctgc acatgattga tgaagaaacc
    18961 ggggaaccga ttgaaccccg tctctctgtg ctgggaatag aggacgaaat cggcacggtg
    19021 cgcatcccgt atttttcaaa tgacggaaac aaagtgctcg agcgggatct tcctattaaa
    19081 aaaatgaacc tgaacggtga agaaacgtac atcacgaccg tgtttgactt gatactggct
    19141 aactacggcg tgaaccgggg catcggcgaa cgatcggctg tctcctatga tgatcctgag
    19201 ccgtttacgc ctgcctggca ggaacaaatg acaggaatca aaaaagaagc tgtcgttaag
    19261 attgccagag agtttgccca aaatgcgatc gatacagacg gccgatccat gattatcgta
    19321 ggggccggca ttaaccactg gttcaactcc gacacgatct accgagcggt gttaaatctt
    19381 gttttacttg taggcgccca aggcgtaaac ggcggaggct gggcccatta cgtggggcag
    19441 gaaaagctcc gacctgctga agggtggcag accattgcaa ctgcaaagga ctgggaaggc
    19501 gtgcccaagc tgcaaaacgg cacctcattt ttctactttg cgacagatca gtggcggtat
    19561 gaggaccagc cgatcagtga tttggcatcg ccgattgctg cgtcatcccg ctacaagcac
    19621 cacgctgatt acaatgtgct ggccgcgcgg ctggggtggc ttccgtctta cccgactttc
    19681 aatcaaaatg gcatcgatct gtataaagaa gctgaaaaag caggggcaga aacacctgaa
    19741 gacgttggtg cgtacgtggc ctcacagctc caagagaaaa aactgaaatt cgcgattgaa
    19801 gatcctgata atgaagtgaa tttcccaaga aatctcttcg tatggcgggc aaatctgatc
    19861 tcgagctcag gaaaagggca tgaatatttt ctcaagcatt tgttagggac gacaaacggc
    19921 ttgatgaatg acgacagcga cagcatccgc cctgaagaaa tcaaatggcg ggagcaggcg
    19981 ccggaaggaa agctcgactt gttaatcaat cttgacttcc gaatggccgg tacggcgctg
    20041 tattccgata tcgtgctgcc ggcggcgaca tggtatgaaa aacacgatct cagcagcaca
    20101 gatatgcatc cgttcattca tccatttgct cctgcgatct cggctccgtg ggaatcgaag
    20161 tcagactggg atattttcaa ggcgctgtca aaagccgttt ccgatctggc agaagaagtc
    20221 gatatggagc ccgtgaaaga agtggttgcg acgccgctcc tccatgacac catgcaggaa
    20281 ttggcccagc cattcggcaa aatcaatgac tggagcaaag gcgaatgtga agccattccg
    20341 ggaaaaacga tgccgaacat ccaagtcgtt gaacgggatt acaaacacat ttttcataaa
    20401 atgactgcac ttggtccgaa cgttgcttta aagccgagcg gaacaaaagg gatgagctgg
    20461 tcaatagccg atgaatatga atcactcaaa cagagattgg gagaaatcac ctcggacagt
    20521 gtcgcaaagg gatgtccaaa tataagtgaa gcaaagcagg cagcagaagc gattttaacc
    20581 ctatcatcca cttcgaatgg aaaggtcgcc gtcaaagcat gggaatcact tgaaaacatc
    20641 acgaacctga agctgaaaga cctggcggaa gaacgcgagg aagaatgctt tacgttcgaa
    20701 caaattacag cccagccgaa aacggtgatc acgtctccag cgttttccgg ctctgaaaaa
    20761 ggagggcgca ggtattcgcc gtttacaacg aatgttgaaa aattaattcc gtggcggacg
    20821 ctgacaggca gacaatccta ttatgtcgat catgaactga tgatggaatt cggtgaaacg
    20881 atggcgacat tcaaaccgat cctccagcat cgcccgtttc tgagcaagcg gcctgatcaa
    20941 gagggaaaag aaatcgtcct taattatttg acgccgcata ataaatggtc tgtccacagc
    21001 atgtattttg attccctgcc gatgctgacg ctgttccgcg gcggaccgac cgtatggatg
    21061 aataaagatg atgcagagga tacggatatc aaagacaacg attggattga atgcttcaac
    21121 cgaaacggcg ttgtcgtcgc aagagcggtt ttgtctcatc ggattcctaa aggaatggcg
    21181 tttatgcacc atgcccagga ccgccacatc aacgtgccag gcacaaagct aacgaataac
    21241 cgcggaggca cccataacag cccgacaagg attcacgtca agccgacaca gatgatcggt
    21301 ggctacgccc agctcagcta cggctttaat tattacggtc caacggggaa tcagcgcgac
    21361 ctgaacgtcg tcatccgcaa gctgaaggag gtcgattggc ttgaagatta aagcgcaaat
    21421 cggtatggtc atgaacttgg ataaatgcat cggctgccac acgtgcagcg tcacctgcaa
    21481 aaacacgtgg acaaaccgtt ccggtgcgga atatatgtac ttcaataatg tagaaacaaa
    21541 gccgggcatc ggctacccga agcaatggga ggaccaggac aaatataaag gcggctggac
    21601 attgaaaaaa gg
//
