LOCUS       NZ_JAQMJT010000011     17027 bp    DNA     linear   CON 28-FEB-2024
DEFINITION  Streptococcus salivarius strain 1001095st1_G4_1001095IJ_161003
            NODE_11_length_69280_cov_29.2774, whole genome shotgun sequence.
ACCESSION   NZ_JAQMJT010000011
VERSION     NZ_JAQMJT010000011.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus salivarius
  ORGANISM  Streptococcus salivarius
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 69280)
  AUTHORS   Chen-Liaw,A.
  TITLE     Human gut microbiome strain richness
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 69280)
  AUTHORS   Chen-Liaw,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2023) Precision Immunology Institute, Icahn School
            of Medicine, 1470 Madison Ave, New York, NY 10029, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.10.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 26.073790043805616x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/28/2024 01:16:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,217
            CDSs (total)                      :: 2,158
            Genes (coding)                    :: 2,099
            CDSs (with protein)               :: 2,099
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 19 of 59
            Pseudo Genes (incomplete)         :: 40 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 13 of 59
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:19:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 52253
            Orig. end                         :: 69280
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAQMJT010000011.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..17027
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..17027
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..17027
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:13776](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..13776)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    183..197
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 19.59"
                     /tool="antismash"
     misc_feature    complement(2131..2149)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 22.57"
                     /tool="antismash"
     gene            complement(2436..2732)
                     /locus_tag="PNU26_RS09080"
                     /old_locus_tag="PNU26_09075"
     CDS             complement(2436..2732)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535478.1"
                     /locus_tag="PNU26_RS09080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_09075"
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_021152512.1"
                     /transl_table=11
                     /translation="MTGLKWFAGGKERREQAVAIIDEVIETIGDKPDYADLKQVLHSYR
                     TELADSRSATPYILSRMSLEISEVVRKDQLTLSPFIEERMAELRKLLAIRYGY"
     misc_feature    3071..3086
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.28"
                     /tool="antismash"
     gene            complement(3502..3902)
                     /locus_tag="PNU26_RS11100"
                     /pseudo=""
     CDS             complement(3502..3902)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014634989.1"
                     /locus_tag="PNU26_RS11100"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKLKSISSRVLRFLGPSLLYWQSFL"
     gene            complement(4232..4399)
                     /locus_tag="PNU26_RS09085"
                     /old_locus_tag="PNU26_09080"
     CDS             complement(4232..4399)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093272.1"
                     /locus_tag="PNU26_RS09085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_09080"
                     /product="hypothetical protein"
                     /protein_id="WP_022495889.1"
                     /transl_table=11
                     /translation="MFKNSKPYLLATILIGMGLANLFRSLDAVHHTINLLGLTLLLYSL
                     LFKKWSQFKK"
     misc_feature    4456..4474
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 23.48"
                     /tool="antismash"
     gene            complement(4474..4599)
                     /locus_tag="PNU26_RS09090"
                     /old_locus_tag="PNU26_09085"
     CDS             complement(4474..4599)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093827.1"
                     /locus_tag="PNU26_RS09090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_09085"
                     /product="hypothetical protein"
                     /protein_id="WP_022495888.1"
                     /transl_table=11
                     /translation="MFDLKSYILGMVFMAVMSFLDKPIMSLLEKHKATNSAKKKS"
     gene            complement(4587..4676)
                     /locus_tag="PNU26_RS09095"
                     /old_locus_tag="PNU26_09090"
     CDS             complement(4587..4676)
                     /GO_function="GO:0005186 - pheromone activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015035.4"
                     /locus_tag="PNU26_RS09095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_09090"
                     /product="ComC/BlpC family leader-containing
                     pheromone/bacteriocin"
                     /protein_id="WP_022495887.1"
                     /transl_table=11
                     /translation="MTRQTNNNFELLDLEALANVEGGDGLCLI"
     misc_feature    complement(4760..4775)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.94"
                     /tool="antismash"
     gene            complement(4818..4985)
                     /locus_tag="PNU26_RS09100"
                     /old_locus_tag="PNU26_09095"
     CDS             complement(4818..4985)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004183367.1"
                     /locus_tag="PNU26_RS09100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_09095"
                     /product="hypothetical protein"
                     /protein_id="WP_002891737.1"
                     /transl_table=11
                     /translation="MLKDKKMYLLAIFLLGFGIANLFRNLDFVHHIINLVGLALITYSL
                     IFSKWERLKK"
     gene            complement(5001..5180)
                     /locus_tag="PNU26_RS09105"
                     /old_locus_tag="PNU26_09100"
     CDS             complement(5001..5180)
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002891738.1"
                     /locus_tag="PNU26_RS09105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_09100"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_002891738.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 5.6e-14,
                     bitscore: 44.6, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTQTMNNFETLDLEALANVEGGGWVKCYAGTIGSALVGSAGGPV
                     GYWGGALVGYATFC"
     misc_feature    complement(5265..5280)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.94"
                     /tool="antismash"
     gene            complement(5340..7085)
                     /locus_tag="PNU26_RS09110"
                     /old_locus_tag="PNU26_09105"
     CDS             complement(5340..7085)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="resistance (resist) ABC_efflux"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096136.1"
                     /locus_tag="PNU26_RS09110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_09105"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_022495886.1"
                     /transl_table=11
                     /translation="MPNQNQWSVFKRLLTYLKPYKFLTFLALAFLLSTTVIKSIIPLVA
                     SYFIDHYLHDMNQAASLILIGYYGLYLLQTLVQYLGNLFFARVSYSIVRDIRRDAFANM
                     EKLGMSYFDKTPAGSIVSRLTNDTETISEMFSGILSSFISAIFIFVTTLYTMMILDIRL
                     TGLIVLFLPLIVLLVNLYRKKSVVIIEKTRALLSDINAKLSESIEGIRIIQAFNQEKRL
                     KKEFDAINEEHLIYASRSMSLDSLFLRPAMSLLKLLGYAVLMAYFGYRGIYLGITAGTM
                     YAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPEQRDTDAKVTEGNI
                     RFENVSFSYDGKHQILDNISFSVNKGETIAFVGSTGSGKSSIINVFMRFYEFQSGRVLL
                     DGVDIRDYSQAELRKNIGLVLQEPFLYHGTIKSNIAMYQDLTDEEIKAAAEFVDANHFI
                     DKLPEGYDAPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANIDSETEAIVQNS
                     LAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIEHGSHEELLALGGTYHKMYSLQAG
                     AMEGA"
     gene            complement(7078..8835)
                     /locus_tag="PNU26_RS09115"
                     /old_locus_tag="PNU26_09110"
     CDS             complement(7078..8835)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002951810.1"
                     /locus_tag="PNU26_RS09115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_09110"
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="WP_195918164.1"
                     /transl_table=11
                     /translation="MFFKESIIAKLWWFFKLEKRRYIVGILALSLVSVLNLIPPMVMGR
                     VIDAITSGKLTNQILLLNLVYLLLAALGMYYLRYVWRMYILATSYRLGQIMRSRLFEHF
                     TKMSPSFYQKYRTGDLMAHATNDINSLTRLAGGGVMSAVDASITALVTLITMFFSISWQ
                     MTLLAVLPLPFMAFATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQEA
                     ELQSFQETNKMTFKKNMHTMKYDSLFDPLVLLFVGSSYVLTLLVGAFMIQTNQITVGNL
                     VTFITYLDMLVWPLMAIGFLFNITQRGNVSYQRIENLLEQESDVQDPAHPLPSIENGRL
                     EYAIDRFAFEDEDTLRDIHFTLDKGQTLGLVGQTGSGKTALVKLLLREHDVNQGAIYLN
                     GHDIRDYRLSDLRSLMGYVPQDQFLFASSILDNVRFGNPELAFEKVEEATKLAQVYEDI
                     KAMPEGFETIIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEHAIFDN
                     LKHTRKDKTTIITAHRLSAVVHADLILVMQNGRIIERGRHEDLLAQGGWYAKTYESQQL
                     EMEGGEVDA"
     gene            complement(8885..9331)
                     /locus_tag="PNU26_RS09120"
                     /old_locus_tag="PNU26_09115"
     CDS             complement(8885..9331)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002891741.1"
                     /locus_tag="PNU26_RS09120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_09115"
                     /product="hypothetical protein"
                     /protein_id="WP_230310599.1"
                     /transl_table=11
                     /translation="MKHLMMTVVTVLTLGATMPMVKADAYQSGRDADIMRERGQVWNYS
                     ESKREPQLANYNTDGRYLSEATNFELYNFVREYKTSDEIRRIWNPKKDESVIHDKDSYS
                     IDDGHKVYNFDSFAYQLPESTDFGKLSYIGHFQLEDGTIYRYWK"
     gene            complement(9434..9961)
                     /locus_tag="PNU26_RS09125"
                     /old_locus_tag="PNU26_09120"
     CDS             complement(9434..9961)
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_process="GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /GO_process="GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884603.1"
                     /locus_tag="PNU26_RS09125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_09120"
                     /product="VanZ family protein"
                     /protein_id="WP_195918165.1"
                     /transl_table=11
                     /translation="MFQLTTWVEKDGELTPKGRKLSQVLVCAYLLCLVLLCWTPQYGLV
                     EGVETPGIQHFGRVVVLLTPFNSLTNFYQLDSLKEIVFVLGQNVTNIFLLSPLILGLLA
                     LYPRFRSWQKVLLATFIMSLTIEVGQVILDLLIDANRVFELDDLWTNTLGGLVALAAYR
                     LLVKLIQTHFKE"
     gene            complement(9963..11072)
                     /gene="rlmN"
                     /locus_tag="PNU26_RS09130"
                     /old_locus_tag="PNU26_09125"
     CDS             complement(9963..11072)
                     /EC_number="2.1.1.192"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /gene="rlmN"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002888240.1"
                     /locus_tag="PNU26_RS09130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_09125"
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="WP_096832658.1"
                     /sec_met_domain="PF04055 (E-value: 1.1e-20, bitscore: 66.9,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 7e-08, bitscore: 24.4,
                     seeds: 32, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEGYKPSIYGLTRDELIDWAMEHGEKKFRATQIWDWLYKKRVQSF
                     EEMTNISKDFIAKLNENFCVNPLKQRIVQESKDGTVKYLFELPDGMLIETVLMRQHYGL
                     SVCVTTQVGCNIGCTFCASGLIKKQRDLTAGEIVAQIMLVQKYFDDRGDGERVSHVVVM
                     GIGEPFDNYDNVLRFLRTINNDNGLAIGARHITVSTSGLAPKIKEFANEGVQVNLAVSL
                     HAPNNDLRSSIMRINRSFPLEKLFEAIEYYIQTTNRRVTFEYIMLNEVNDHPENAQELA
                     DLTKKIRKLSYINLIPYNPVSEHDQYSRSTKERVAAFYDVLKKNGVNCVVRQEHGTDID
                     AACGQLRSNTMKRDRQKAVAEASGKSEGK"
     gene            complement(11136..11864)
                     /locus_tag="PNU26_RS09135"
                     /old_locus_tag="PNU26_09130"
     CDS             complement(11136..11864)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621927.1"
                     /locus_tag="PNU26_RS09135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_09130"
                     /product="YutD family protein"
                     /protein_id="WP_002891743.1"
                     /transl_table=11
                     /translation="MRKEILPEMFNYNKYPGPDFVYVADLLKSDACQFTVLENHKDALD
                     ATRFGQRFSEIMLKYDYIVGDWSNDQLRLKGFYEDERPNTRKTDRISRLSEYLREYCAF
                     GCAYFILKNEEPQLIVFEEETEPRRKRRRSNNRRHSSQESRDREHNASQKNKGQKASAK
                     QKPDKSKRRDRDQEGPNFEKIKRRPKFKSRSEDGGRERKSNFKKSQKQAPKSKGQNTPS
                     KKQSQTKSDGQHFTIRKKGN"
     misc_feature    11819..11832
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 22.7"
                     /tool="antismash"
     misc_feature    complement(11819..11834)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 22.94"
                     /tool="antismash"
     gene            complement(11919..13274)
                     /locus_tag="PNU26_RS09140"
                     /old_locus_tag="PNU26_09135"
     CDS             complement(11919..13274)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1283: 2`,3`-cyclic-nucleotide 2`-phosphodiesterase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884653.1"
                     /locus_tag="PNU26_RS09140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_09135"
                     /product="metallophosphatase"
                     /protein_id="WP_175057772.1"
                     /transl_table=11
                     /translation="MKDTIRILHINDLHSHFEQYPQLKRAVDDLSQTDRELIKVDLGDN
                     VDKSHPLSDATAGRFNVALMNELGIDYATIGNNEGIGLAKNELDCLYEQAKFQPIIGNL
                     KDEGGQPKWAKPYLIHKTKAGTNIAFLAYTFPYYITYAPNGWQVLEPMTRLEEDLARPE
                     VRDSDIVVVLSHLGVRYDEQIAETYPQVNLIIGSHTHHLFEEGKLINETYLAAADRYGY
                     YLGCIDLTVENGRLIECQIEAIPTKSYILDLDKEDETFIKAMREEGHRRLAQKKVANLG
                     RELDFDETCQLVLQAVCQETDSQLTLLNTGLIMKTLGPQVTMADLQEALPHQMRMARLV
                     VTGQELKGICREVFTKAELLKNQAIKGMGFRGKTFGEVITGNFAYKNGNLLYNRRVVDS
                     NEKFSLVLVDQYYFASYFPTIKEKEVTLLFPDLLRELVAKYLGKNGANWDKI"
     gene            complement(13545..13776)
                     /locus_tag="PNU26_RS09145"
                     /old_locus_tag="PNU26_09140"
                     /pseudo=""
     CDS             complement(13545..13776)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884558.1"
                     /locus_tag="PNU26_RS09145"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_09140"
                     /product="hypothetical protein"
                     /pseudo=""
                     /sec_met_domain="Bacteriocin_IIc (E-value: 3.2e-10,
                     bitscore: 32.6, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTQIINNFNSLNSEDLSTVKGGACSFWGATAAVGVGAVGGAVKG
                     GIQTGTWQGAALKGYWLWY"
     misc_feature    complement(13863..13878)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.94"
                     /tool="antismash"
     gene            complement(14141..15484)
                     /locus_tag="PNU26_RS09150"
                     /old_locus_tag="PNU26_09145"
     CDS             complement(14141..15484)
                     /EC_number="3.6.4.-"
                     /GO_function="GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1122: ATP-dependent
                     RNA helicase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /locus_tag="PNU26_RS09150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_09145"
                     /product="DEAD/DEAH box helicase"
                     /protein_id="WP_175057773.1"
                     /transl_table=11
                     /translation="MSFKDFNFKTFIQEALDEIKFKEPTPVQQKLIPVVRSGRDLVGES
                     KTGSGKTHTFLLPIFEKLNPESGDVQVVITAPSRELATQIYQATKQIAKHSDTEIRVVN
                     YVGGTDKQRQIEKLKVAQPHIVIGTPGRIYDLVKSGDLAIHKAHTFVVDEADMTMDMGF
                     LDTVDKIAASLPKEVQILVFSATIPQKLQPFLKKYLTNPVMEQIKTSTVIADTIDNWLV
                     STKGRNKNEQILEMLKGMQPYLAMIFVNTKERADDLHSYLVSNGLKVAKIHGGIPPRER
                     KRIMNQVKKLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTGRNGLSG
                     TAITLYQPSDDSDIRELEKMGITFEPKVYKDGEFQDTYDRDRRANREKAYQKLDTEMIG
                     LVKKKKKKIKPGYKKKIQWKVDEKRKRERRAANRAKGRAERKAKKQSF"
     gene            15685..16934
                     /locus_tag="PNU26_RS09155"
                     /old_locus_tag="PNU26_09150"
                     /pseudo=""
     CDS             15685..16934
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1206: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263494.1"
                     /locus_tag="PNU26_RS09155"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_09150"
                     /product="ISL3 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRLIKNTTELIGIKDQNIKISLVFETDTHIEIQAKLDYPAPPCPH
                     CHEMMIKYDFQKSSKIPLLEQAGTPTLLRLKKRRFQYKVCKRVTVAETSIVEKNHQISN
                     LVRQKVAQLLTEKVSLTDIARRLRVSTSTVYRKLDQFTFKEHYDKLPAVMSWDEFGFKK
                     GELAFVAQNYETNKLITILDNRCQTTIRNYFLKYPLKVRQQVQFITMDMSGAYMPLARR
                     LFPNAEIIIDRFHIIQHLGRAFLKTRIAIMNQFDKKSLPYRALKNHWRLFQKDSRKLSC
                     KSFHSKTLGQTLSPHEVVRKTLDFSEELANYYNLYQLLLFHFQEKRVDEFFELIEENMS
                     KVNHYFQTVFRTFLRHKQYIKNALETDYSNAKLEATNKLIKDIKRLGFGFRNFINFKKR
                     VFIILNIKKEKTYQVLS"
ORIGIN
        1 tgttagtcct gaaatagctt gtcaggaact ccgttctttg ggctttgaag tctctctaga
       61 tgctagtgcc aaagccaatt ttgaatcttt tgttcgcaag cacttccttt tctttgaaga
      121 tactgattta gctttgaaaa attggattgc tgattcagag actgacctcc taaccttctt
      181 ccagtcagac cgtcccttga ctgctgacgt ctttggtttg gtagcccttc aaatgctagg
      241 atttgtccct aatgtggact ttacagacag tgctgctttc ctagaggaaa tggcttttcc
      301 aattactttt gatggtagcc ttaataacct acatcaattg ctagcaacac gcacacagtc
      361 tggcaatacc ttaattgatc agttagtagc tcaggattta atccctgtaa gtaataacta
      421 cgtcttcttt aatggtaaga gcttggcaac atttgacact aatcagcttc accgtgaggt
      481 ggtttatgtc gaaacacctg tagataccga taaagatggg cagttagact tggtcaaggt
      541 gactatctta cgtcctgatg tggacttccc ggttccagcc atgatgaccg caagccctta
      601 tcaacaaggg actaacgagc ctgcttcaga taagctcacc cacaagatgg aaggtgattt
      661 gctcgtcaaa ccaacaggtg aaatctccct tagccaacca gaaatcaagg ccccagaggc
      721 agaccttacg cctatcaatc ctgtcacaaa ggctcaggag cgttttgctc acacagatac
      781 ctatacgctt aacgattaca tgttggctcg tggtgtggct tctatctatg tatcgggtgt
      841 cggtaccttc aattctgaag gcttcatgac ctctggagac tatcaacaag ttctggccta
      901 taaagctgtc attgactggc tcaatggtcg tgcacgcgcc tttactagtc gtagtagaca
      961 gcataccatc actgcagatt gggcatctgg taaggtgact actaccggac tttcatactt
     1021 aggtaccatg tccaacgccc ttgctacaac tggtgtcgac ggtttggaga tggtcatcgc
     1081 tgaagcaggt atttcttctt ggtacgacta ctatcgtgaa aatggactcc tcgtcagtcc
     1141 tggaggatac cctggtgaag atctcgacac cttgactgaa tttacctatt ctcgtgccct
     1201 attagctgga gaatacctac gccaccaaaa agactaccaa gcctacctca aagagctaag
     1261 cacagccatt gatcgtaagc atggggacta tagccaattc tggcatgacc gtaactatgt
     1321 gcagttcgca gatcgtgtta aagctactgt tgtctttaca catggtagcc aagactggaa
     1381 tgtcaaaccg attaacgtct atcaaatgtt caatgctctt ccagactctc ttgacaaaca
     1441 cctcttcttc cacaatggtg cccacgttta catgaatgct tggcagtcta ttgacttccg
     1501 agaaagcatg aacgccttga tttgtcagaa actccttggt ttggagaatg gctacacact
     1561 gcctactgtt atctggcaaa acaaccagtc tgaacagaca tgggaagttc tcgataactt
     1621 tggacacgac aatggtaaaa acattcagct tggtgagact gaagctagta tcgctaacca
     1681 ttatgaagag gaaacctttg ccaaatacgg taaagcttac caaagcttca aggatgacct
     1741 ctttgcagac aaagccaacg ccattacctt ggactttgaa ctggaccaag acatccagat
     1801 taatggccgt gtccacctag agctcaaggt taagtctagc acaaaccgta gtcttatctc
     1861 ggcccaggtt ctggaaatgg gagataaaaa atacctagct ccaatacctg aattaaaacg
     1921 catgagttta gataacggac gtctcttcaa ggaagaagcc ttacgtgaac tgccattcaa
     1981 gcaggccaaa taccgtgtca ttaccaaagg acaccttaac ctgcaaaatc gtaaagacct
     2041 tcttaccatt gaagatgtta cgccaaatga atggatgact atcggtttgg acttacaacc
     2101 aaccatctac aaactcaaca agggcgacaa actccgactc gttctctata ccacagactt
     2161 tgaacacacc attcgtgaca atagtgacta cgaattaact gtggacctaa gccaaagtca
     2221 gatgacattg ccttattagt ctttatcaca aaattcccat aggatgatga ctcctatggg
     2281 aacttttttt gtgtgtaaat aagcaagatt taggcttcaa ttgttacgat ttattttaat
     2341 atcatttaaa caaatgatat agccaagaca gtggaataac acaaattctc aatggacaac
     2401 tcaaccttaa aaccgagaaa tcctccctaa tgacatcaat agccataacg aatcgctagc
     2461 aattttcgca actctgccat tcgctcctcg ataaagggag acaaggttag ctggtctttt
     2521 ctgaccactt cggatatttc cagtgacatc ctgcttaaga tatagggagt ggctgaacga
     2581 gagtctgcaa gttccgttcg gtaggagtgc aggacctgct tcaaatcagc ataatctggc
     2641 ttatctccaa tagtttcaat tacttcatcg atgatagcta ctgcttgttc tcgtctctct
     2701 ttgcctcctg caaaccattt aagtcctgtc attaagaaaa cctccttatt tctttcaact
     2761 aatttttcaa tcacatcttt agtatagcat tcaagtcttt ctatcagagc tagcgtccca
     2821 aaaagtggct atatgtccta aatggtagcc aaatccagat tggatgctga atagatagag
     2881 catttaaatc tatattccca atgactactc ctaggattgc tgatacgaca cgtaacgaac
     2941 gtaacacagc agaactaatt ttttgtaata tcaccatggt aattcctttt ctacttgagt
     3001 catttacata aactactagg gcgttatcta aagaatgatt ggctattatt ctgaatctag
     3061 catggctaaa gacacgaatc tgtcactttc catgctgctt aaaacaaata catcacttct
     3121 catttctaaa aatcagctga ctaagatcat cagagaacga taaagaaaca taaagtgaca
     3181 aggtataaaa taaaaatgtt ctttcttgat gccagaaaaa tgttatcaca cattgaatac
     3241 agacagccaa gataacttgt aggataaata ttatgtattt tctagaaaaa acagattgtt
     3301 ccaatagtaa tcctcctttc atgacatatt ttgaagaccc agaccttgca agttttgtct
     3361 atacgataga agaatatccg tcaagccata gaaatcctac tctaattttc acatttttca
     3421 ttaatcactt tcttattata gcgtttttat gcactttatg caacgtaaat tgaataatgt
     3481 cacaaatggt tactatattt tctatctttt aaaatatttt ttgacttgca agtaagctag
     3541 aaaatcacag agaattaaca aaagcattaa tacgactagt actatgcggt ctgtggtatc
     3601 actgaatgtt agtaatgtgt aaaactctac acaaagcact agaaatgaga tgaccagaaa
     3661 gacaatgaag gactgccata ttgttaacgg atttactgaa tcgggacgct tcttaagcaa
     3721 acggtagagc agtccattta caaaaatgac tactaaagct agtgctccga ctgataacca
     3781 aatctctggc gaagtcttac ttttgtagag agaaatactg gtacttataa gaaagactgc
     3841 caatacagca gtgatggtcc taaaaatcgc aatactctcg aactaatact ctttaacttc
     3901 atgataaaac acttaagcta tgaataacct cagtcagtct tgtaaaccga ataagctatt
     3961 acatttaact ctattccttt ctgtattttt ttatactgct gtaagatctc aagtaatccc
     4021 ttagcgatta aattctactg gagatgtaat acaatagcgt cattagcact gatattttaa
     4081 ataagcgaat atacatatct ttattttttc tattacaaaa atatgcaatc aaatataata
     4141 caatctccgc tatcattagc aagaataatt ttacttcggg aacatcaaaa ataagcattt
     4201 gcaagcacct cacgttcaaa ggggcaaaag gttacttttt aaactgtgac cattttttaa
     4261 aaaggagact ataaagaagc aaggttagcc ccaacaagtt aatggtatgg tggactgcat
     4321 ccaaacttct aaatagatta gcgaggccca ttccgatgag aatggtagcc agtaaatacg
     4381 gttttgagtt tttaaacata cttttctcct taaagtgttt ttagcgttct gtgcaaagct
     4441 attcacagac tagcaattga caaggtatct caattaggat ttctttttag cggaattggt
     4501 agctttgtgt ttttcaagca atgacataat aggcttatcc aaaaaactca tcacagccat
     4561 gaaaaccatc cctaaaatat aacttttcaa atcaaacata atccatcacc accttcaacg
     4621 tttgcaagtg cctcaaggtc aaggagttca aaattgttat ttgtttgcct tgtcatagca
     4681 acaacctcct aaactatttt ttagcttttt tctctcaagc tatgaatttt attataggtt
     4741 gtaggccgac aaaaaagtag tgacataaat gtcactactc gtttttgatt tttaggaact
     4801 ctacatttcg aatacttcta ttttttgagt cgctcccatt tgctaaaaat cagactataa
     4861 gtgattaatg ccaaccctac caaattaatg atatgatgaa caaaatctaa atttctgaaa
     4921 aggttagcaa tgccaaatcc taaaaggaaa atggctagta aatacatttt tttatcttta
     4981 agcatagaat cacctaaata ttagcagaat gtggcataac caactaaagc tcctccccag
     5041 taacctactg ggcctcctgc tgatcctact agagcagatc cgattgtgcc ggcataacac
     5101 ttaacccatc caccaccttc aacgtttgca agtgcctcga gatcgagtgt ttcaaaattg
     5161 ttcattgttt gtgttgtcat tctaacaacc tcctaaaata ttatttttta gctttttctc
     5221 tcaagctatg gttttattat aggttgcaag ccaacaaaaa agtagtgaca tgtatgtcac
     5281 cactcgattt tgatttttaa gaaatctttt cgaatataga atctatggtc tagattagct
     5341 tacgctcctt ccatagcgcc tgcttgtagg ctgtacatct tatgataggt gccaccgagg
     5401 gctaggagtt cctcgtggct accgtgttcg atgatgcggc ccttgtctaa gacatagatg
     5461 cagttggcat cttggatagt cgataggcgg tgggcaatgg caatggttgt acgtccttga
     5521 cgcatttttg ctagagagtt ttgaacaata gcctctgtct ccgaatcgat attggctgtc
     5581 gcttcatcca aaatcaagat tttaggctgg ctagcaacgg tcctagcaaa ggctagaagc
     5641 tgacgttgac cggttgaaaa acttgaacct cgttcggaaa ctggagcatc ataaccttct
     5701 ggaagtttgt cgataaagtg gttggcatcc acaaactcag cagcggcttt gatctcttca
     5761 tcagttaaat cctgatacat tgcaatattg gatttgattg taccatgata gagaaagggt
     5821 tcctgaagga caagaccaat gttcttacgc agctctgcct gactataatc gcgaatatct
     5881 acaccgtcaa gaagaactcg tcctgattgg aactcataaa aacgcatgaa tacattgata
     5941 atagaggatt ttccggaacc tgtagaaccg acaaaggcga tggtctcacc tttattgaca
     6001 gagaaggata tgttatctaa aatctggtgt ttaccatcat aggagaaaga gacattttca
     6061 aagcgaatat tcccctcagt aaccttggca tctgtgtctc tctgctcagg ctcataggtg
     6121 cgctcgtcaa tcaaagcgaa gacccgacct gctgagacca tcgaggtttg caaggttgag
     6181 aagttttgtg tcacttcaat caaaggatca aagaggcggt taatgtactg gataaaggca
     6241 tacatggtac ctgctgtgat acccagatag atgccacgat agccaaaata agccatgaga
     6301 acagcatagc ccaggagctt cagtaagctc atggctgggc gtagaaagag agaatccagt
     6361 gacatggaac ggctggcgta aatcaggtgt tcctcattaa tggcatcaaa ctcttttttg
     6421 agacgtttct cttggttaaa ggcctgaatg atgcgaatcc cttcgatact ctctgacagt
     6481 ttggcattta tatctgaaag caaggctctg gtcttttcaa tgatgaccac tgattttttg
     6541 cggtagagat taaccaaaag aacaatcaga ggaagaaaga gcacaataag cccggtcaga
     6601 cgaatgtcca agatcatcat ggtgtaaagg gtggtaacaa agataaagat agccgaaata
     6661 aagctagaca gaatccctga gaacatctcg ctaatagtct ctgtatcatt ggtcaagcgg
     6721 gaaacgatag agcctgctgg agttttgtca aagtaagaca tacctagttt ctccatattg
     6781 gcaaaggcat ctcggcgaat atccctgaca atactgtagg acacacgcgc aaaaaagaga
     6841 tttcccaaat actggactag cgtctgtaag agataaagac catagtaacc aatcagaatc
     6901 aagcttgccg cctgattcat atcatgcaga tagtggtcga taaaataaga ggccacaagg
     6961 ggaatgatac tcttgataac cgtagtggac aagaggaagg ccaaagctag aaaggtcaag
     7021 aatttgtaag gcttgagata agtcaagaga cgcttaaaca cagaccattg gttttgatta
     7081 ggcatcaact tctcctcctt ccatttctaa ctgttgagac tcataggtct tggcatacca
     7141 accaccctga gctagtaagt cctcatgacg gccccgttca atgatacgac cattttgcat
     7201 gactaagatg aggtcagcgt gcactaccgc actaagacga tgggcggtaa tgatcgtcgt
     7261 cttgtccttg cgggtatgct tgagattatc aaaaatagca tgttctgtct tggcatcaac
     7321 cgcagataag gagtcgtcca aaatcaaaat atctggatcc aaaatcatgg cacgactcat
     7381 ggccagacgt tgtttttgac cacctgatag gctgacaccc ttttcaccaa tgattgtttc
     7441 aaagccttca ggcatagcct tgatatcctc ataaacttga gccaacttag tcgcttcttc
     7501 caccttttca aaggcgagtt caggattacc aaagcggaca ttgtctaaaa ttgaactggc
     7561 aaagagaaac tggtcttgag gaacatagcc catcaagcta cgcaagtctg ataggcgata
     7621 gtctcggata tcatgtccat ttaaatagat ggctccttga ttgacatcgt gctcccgcaa
     7681 gagcaacttg acaagagccg tctttcctga accagtctgc ccaaccaaac caagggtttg
     7741 gcccttgtcc aaggtgaaat gaatatctct caaagtatcc tcatcctcaa aagcaaagcg
     7801 gtcaatcgca tattctaaac gaccattttc aatgcttggg agaggatgag caggatcttg
     7861 aacatctgat tcctgctcca gaagattttc aatacgttgg taggatacat ttcccctctg
     7921 tgtgatatta aagagaaagc caatggccat caaaggccaa accaacatat ccaaataggt
     7981 aatgaaggtt actaaatttc cgaccgttat ctgattagtc tgaatcataa aggccccgac
     8041 aagaagggtt aaaacataag atgagccaac aaagagcaag accaaaggat caaaaaggct
     8101 gtcatacttc atggtatgca tattcttttt aaaagtcatc ttattggtct cttggaagga
     8161 ctgaagctca gcctcctgat agccaaaaga cttggttacc ttgatccctg ataccgactc
     8221 ctgaacctta ttattaagtt ctgaaaaggc tgcctgggat tcaccaaaag ctttatgggt
     8281 cttgcgtccc aaacgactag tcgcaaaagc catgaagggt aggggtaaga cagccaacag
     8341 ggtcatctgc caggaaatgc tgaagaacat ggtgattaag gttaccaagg ctgtgataga
     8401 cgcatctacg gctgacatga cacccccacc agccaagcgg gttaaggagt tgatatcatt
     8461 agttgcatga gccatgaggt cccctgttcg gtacttttga taaaaagaag gcgacatctt
     8521 ggtaaaatgc tcaaaaaggc gtgacctcat aatctgaccc aagcgataag aggtcgctaa
     8581 gatatacatc cgccaaacat agcgcaaata gtacattcct aaagctgcca aaagcaaata
     8641 cacaagattc aaaagcaaga tctgattggt caatttcccc gatgtaatag catcaatgac
     8701 ccgccccata accataggag gaatcagatt gagtaccgaa accagactca gtgccaaaat
     8761 cccgacaata taccggcgtt tttctaactt gaaaaaccac caaagttttg caatgattga
     8821 ttctttaaaa aacaagtgat tccccctatc cttacaaacg agtgattata attctaacaa
     8881 aaggttattt ccaatagcga tagattgtgc catcttctaa ctgaaagtga ccaatataag
     8941 ataacttccc aaaatcagta gattctggca attgataagc aaaggagtca aaattataga
     9001 ccttgtgccc atcatcaata gagtagctat ccttatcgtg gataactgat tcgtccttct
     9061 tggggttcca aatgcgtctg atttcatcag atgtcttata ctctctgaca aaattgtata
     9121 attcaaagtt agttgcctca ctgagatagc gaccatcggt gttgtagttg gctaattgag
     9181 gctctctctt agactcagaa taattccaaa cctgtcctct ttctcgcatg atatcggcat
     9241 cacgtccgct ttgataagca tctgctttaa ccattggcat tgttgctcct agtgtcaata
     9301 cggtgactac tgtcatcatt aagtgtttca ttgtttctcc aaaagtgttt attcattttt
     9361 ccctcttttg aatgtgttgt atttgtatga tagaaaataa gtgaaacatg agacgcgtaa
     9421 gctcccaggt tttttactct ttaaaatgcg tttgaatcag ttttacaaga aggcggtagg
     9481 cagctaatgc caccaaacct cccaaggtat tggtccataa atcatctaac tcaaagacac
     9541 gattcgcatc aatgaggaga tctagaataa cttgcccaac ctcaatcgtc aaagacatga
     9601 taaacgtggc taaaaggacc ttttgccaag acctaaatct ggggtacaag gctaaaagtc
     9661 ctaaaatcag cggactcagc aagaaaatgt tggtcacatt ttgcccaagc acaaagacaa
     9721 tctctttaag actatccaac tgataaaagt tggtcaaact attgaatggg gtcaagagga
     9781 ccactacacg accaaaatgt tgaatcccag gagtttcaac accttcaact agtccatact
     9841 gtggtgtcca acaaaggagt actaaacaaa gaagataagc acagaccaaa acttggctta
     9901 gctttcttcc ttttggtgtt agttctccgt ccttttctac ccaagtcgtc agctgaaaca
     9961 tcttacttac cttctgattt accagaagct tctgctacag ccttttgacg gtcacgtttc
    10021 atagtgtttg aacgcaattg tccacaagca gcatcgatat ctgtaccgtg ttcttgacga
    10081 acgacacagt taaccccgtt tttcttgagc acatcgtaga aggccgccac acgttccttg
    10141 gttgaacgac tatattggtc gtgctctgat actgggttat atggaatcaa gtttatgtat
    10201 gacaacttac ggattttctt agtcaaatca gccaactctt gagcgttttc agggtggtca
    10261 ttgacctcat taagcatgat gtactcaaaa gttacacgac ggttggttgt ctggatgtaa
    10321 tactcgatcg cttcaaagag tttttcaagt gggaatgagc ggttaatacg catgatgctt
    10381 gaacgaaggt cattgttagg cgcgtgaagt gatacggcaa ggttaacttg aacaccttcg
    10441 ttagcaaatt ccttaatttt tggtgccaaa ccagatgttg acactgtaat gtgacgagct
    10501 ccaatagcaa gaccattgtc attgttaata gtacgcaaga aacgaagcac attgtcatag
    10561 ttatcaaatg gctcaccgat acccataaca accacgtggc tgacacgttc accatcgcca
    10621 cggtcatcaa aatatttttg taccaacatg atttgtgcca caatctcacc agcagtcaag
    10681 tcacgttgtt tcttaatcaa accactggca cagaaagtac aaccgatgtt acatccaacc
    10741 tgagttgtca cacagacaga caaaccgtag tgttgacgca tcaaaacagt ctcaatcaac
    10801 ataccgtcag gcaattcaaa aagatactta actgtaccat ctttagattc ttgaacgata
    10861 cgttgtttca atggatttac gcagaaattc tcattcaact tggcaatgaa atctttagaa
    10921 atgttggtca tttcttcgaa agactgcaca cgcttcttgt agagccaatc ccaaatttga
    10981 gtcgcacgga atttcttttc accgtgttcc atagcccagt cgatcaactc atcacgagta
    11041 agaccataaa tagaaggttt gtagccttcc atatcctcta acttctgacg tttacggcgt
    11101 tctggttttg cttgtgttgt tacttctgac atagtttaat ttcctttttt tctgattgta
    11161 aaatgctgac catctgactt ggtctggctt tgtttcttcg atggggtgtt ttgaccttta
    11221 gattttggag cttgcttctg actcttcttg aagttagact tacgctcacg gccaccgtcc
    11281 tcggatctag atttaaactt aggacgacgc ttgattttct caaagtttgg gccctcttga
    11341 tccctatctc ttcgtttgga cttgtctggc ttttgcttag cagaagcttt ttgcccctta
    11401 ttcttttgag aagcattgtg ctctctatct ctagattcct gagaagaatg acgacgatta
    11461 ttagaacgac ggcgcttgcg acgaggctca gtctcctctt caaagacgat taactgtggc
    11521 tcttcatttt tcaaaataaa gtaggcacaa ccaaaggcac agtattcacg taaatactct
    11581 gacaatcggc taatgcggtc tgtcttacgc gtattagggc gctcgtcctc gtaaaatccc
    11641 ttaaggcgaa gctggtcatt agaccagtcc ccaacaatat aatcatactt gagcatgatt
    11701 tcagaaaaac gttgcccaaa gcgggtagcg tctaaagcat ccttgtgatt ttcaagaacc
    11761 gtaaactgac aggcatctga tttcagaagg tcagccacat acacaaagtc tggccctgga
    11821 tatttattat aattaaacat ttctggcaag atttctttac gcatgtgcca tcctttctgc
    11881 tctctcttat tttctatcct gatgctgtaa taatgtctct atatcttatc ccaattggca
    11941 ccattttttc ctaaatactt ggccacaagt tcccttaaca aatctggaaa aagaagagta
    12001 acttcctttt ccttaatagt agggaagtaa gaagcaaagt agtactggtc cacaaggacc
    12061 aaactaaact tctcattgga atctacaacc cttctattat acaacaaatt accgtttttg
    12121 taagcaaaat tccccgtgat tacttcacca aatgtcttcc ctctaaatcc cataccttta
    12181 atagcttgat ttttaagaag ttcagcctta gtaaaaacct cccgacagat tcctttgagc
    12241 tcttgtcctg tcacgactag acgtgccatt cgcatctggt gaggaagggc ttcttgcagg
    12301 tctgccatgg tcacttgagg acctaatgtt ttcatgataa gcccagtatt taaaagcgtt
    12361 aattggctat cggtctcctg acaaacagct tgtaaaacca attgacaggt ctcatcaaaa
    12421 tctagttctc gacctagatt agctactttt ttctgagcta aacgacgatg gccttcctca
    12481 cgcatggcct tgataaaggt ttcgtcctcc ttgtctaaat ccaagatata ggacttggtg
    12541 gggatagcct caatctgaca ttctatcaat cgcccatttt ccacagtaag gtcaatacag
    12601 ccaagataat aaccatatct gtcagcagct gccaaataag tctcattaat caacttgccc
    12661 tcttcaaaaa ggtggtgggt atggctcccg ataataagat tcacctgagg gtaagtctct
    12721 gcaatctgct catcataacg tacacccaga tggctaagaa cgacgactat gtctgaatcc
    12781 cttacctcag ggcgagccaa atcctcttcc aaccgagtca taggctctaa cacctgccaa
    12841 ccgttaggag cataggtgat gtaatatggg aaggtatagg ccaagaaagc aatgtttgta
    12901 cctgctttag ttttgtggat aagatagggc ttagcccatt ttggctgtcc cccttcatcc
    12961 ttgagatttc ctataattgg ctgaaacttg gcctgctcat aaagacagtc tagctcattc
    13021 ttagctagac caatcccctc attatttcca atagtcgcat agtctatccc cagctcattc
    13081 atcagggcta cattaaaacg cccagcggtc gcatcagata gaggatgaga cttatccaca
    13141 ttgtccccaa gatctacctt aatcaactct ctatccgtct gactcaaatc atccacagct
    13201 cgcttgagtt gtggatactg ttcaaaatga gagtgtaaat cattaatatg aagaattcga
    13261 atcgtgtctt tcatgactcc tattgtagca cttttgccat aaaaaaactt gacaatcatt
    13321 ttgccaagcc tttaactatt tttcaaacag gaaggtattc ctagttaact ttctttttta
    13381 aatattcttc tgcgagatga tagatgtcat ctgccaggga tatactgaca tagatgcaca
    13441 caattgagaa aacttctcct ctaaagtgtt gccaagcaag gaatataagc ccataaatta
    13501 ggagagcaag tactatttgg ccaaaaaatt gactcagtaa tcgcctaata acgacaagta
    13561 aggccataag taatcccacc tttaatacca taaccaataa ccctttaagg ctgcaccttg
    13621 ccaagtacct gtttgaatac cacctttaac ggctcctcca acagccccga cacctacggc
    13681 agctgtagct ccccaaaaac tacatgctcc ccccttaaca gtagaaagat cttcggaatt
    13741 aagtgaatta aaattgttaa tgatttgtgt tgtcatagta acaacctcct ttaaaatatt
    13801 ttgttctacc cttttctcaa aagctatgat tttattatag gtagacagcc aacaaaaaaa
    13861 tagtgacatt tatgtcacta cttgattttg agagttaact caactagtaa cctcaaactt
    13921 acaaattatt ttactttaaa cggaaagctt cttaaaaata gctgtgataa aactatttaa
    13981 aatctgtcat agtttcagtt tttaagaaat acttaattat ttttccagca tatactgaga
    14041 ctttccaaaa agtttgataa taatcaaaaa agctgaaaca ctttggtttc aacctcttga
    14101 atatttaaat taaaatttgc tttcagctaa aagccacggc ttagaaactt tgcttcttag
    14161 ctttacgttc agcacgtccc ttagcacggt tagccgcacg acgttcacgt ttgcgttttt
    14221 cgtcaacctt ccactgaatt ttcttcttgt agccaggctt gatttttttc tttttcttct
    14281 taaccaaacc gatcatttcc gtatcaagtt tttgataggc tttttcacgg ttggcacgac
    14341 ggtcacggtc ataggtatct tgaaactcgc cgtccttata gactttaggt tcaaaggtaa
    14401 tccccatctt ttcaagctca cgaatatcgg agtcgtcact tggttgataa agggtaatgg
    14461 ctgttcctga caaaccatta cgtcctgtac gccctacacg gtgaacaaag aaggacaaat
    14521 cttgagggat agcatcattg ataacatggc taaccccttc aatatcaatc ccacgcgccg
    14581 caaggtcagt cgctacaatg tactcaaaat caagcttctt gacttgattc atgatacgct
    14641 tacgttcacg tggtggaata cctccatgaa ttttggcaac cttaagacca tttgacacta
    14701 ggtagctgtg caaatcgtca gcacgttcct tggtattaac gaaaatcatt gccaaatatg
    14761 gctgcattcc cttaagcatc tcaagaatct gttcattctt gttgcgcccc ttggtagata
    14821 ccaaccagtt atcaatggta tctgcaataa ctgttgaagt cttgatttgc tccatgactg
    14881 gattggtcaa atacttcttc aagaatggtt gcaatttttg aggaatggtt gctgagaaga
    14941 ccaaaatttg aacttcctta ggcagacttg ccgcaatctt gtccacagta tccaagaatc
    15001 ccatgtccat ggtcatatca gcttcatcaa caacaaaggt atgggccttg tggatagcca
    15061 agtcaccaga cttaactagg tcatagatac gtcctggtgt cccgataaca atatgaggtt
    15121 gggctacctt gagtttctca atctgacgtt gcttgtctgt accacccaca tagttaacca
    15181 cacgaatttc tgtatcagag tgtttagcaa tctgttttgt tgcttggtaa atttgtgtcg
    15241 ccaattcacg tgatggtgct gtaataacaa cctgaacatc accactctct ggatttagtt
    15301 tctcaaaaat tggcaagagg aaggtatgtg tcttccctga acccgtcttt gattcaccaa
    15361 ccaaatcacg acctgaacgg actacaggaa tcaatttctg ttgaacaggt gttggctcct
    15421 taaacttaat ctcgtcaaga gcctcctgaa taaatgtttt aaaattaaaa tctttaaatg
    15481 acatgtttac ttcttctttt ctatctagtc tatccctatc attataacag aaaaagagca
    15541 tttctgctct tttcatttat ttcggctcta taatttctgt agtgggtaaa accactgtag
    15601 agattataga gctttttgag tgtacacaaa aagtcccata agaactataa tgaaaagcga
    15661 ccaaaccatc attaggaaga acttatgaga cttattaaga ataccacaga attaatcgga
    15721 attaaagacc aaaatatcaa gatttcactt gtttttgaaa ctgacactca tatcgagatt
    15781 caagcaaaac ttgattatcc tgcaccacca tgccctcatt gccatgaaat gatgatcaag
    15841 tacgactttc aaaaatcttc taaaatccct cttcttgaac aagctggaac tccaacacta
    15901 ctacgtctga agaaacgtcg cttccagtat aaagtgtgta agagagtaac ggtggctgaa
    15961 acctcaatcg ttgagaaaaa ccaccaaatc tccaaccttg tccgacaaaa ggttgcacaa
    16021 cttctaactg agaaggtatc actaacggat attgccagaa gacttcgtgt atcgacgtcc
    16081 actgtctatc gtaaacttga ccagtttact ttcaaggaac attatgacaa actcccagct
    16141 gttatgtcct gggatgagtt tgggttcaag aaaggggaac tcgcttttgt ggcgcaaaac
    16201 tatgagacca acaaactcat aaccatcctt gataatcgct gccaaactac tatacgaaac
    16261 tattttttga agtatccatt gaaagtccga caacaggtac agtttatcac gatggacatg
    16321 tcaggagcct atatgccact agctcgaaga ctttttccaa acgccgaaat cattattgat
    16381 cgtttccaca tcatccagca ccttggtcga gcctttttaa agacaagaat tgccattatg
    16441 aaccagtttg ataagaaatc actcccctac cgagccttga aaaatcactg gcgactcttc
    16501 caaaaggaca gccgtaagtt atcttgtaaa tcatttcact cgaagaccct tggtcaaacc
    16561 ttaagtccac atgaagtcgt taggaagaca ctagactttt cagaagaact cgccaattat
    16621 tataacctct atcagctctt actttttcac tttcaggaga agagagtaga tgagtttttt
    16681 gagttaatag aggagaacat gagcaaggtc aatcactact ttcaaacggt ctttaggact
    16741 tttcttagac acaagcaata catcaagaat gcgctagaaa cagactattc aaacgcaaaa
    16801 cttgaagcaa ctaataagtt gattaaagac atcaaacggt tgggcttcgg ttttagaaac
    16861 ttcatcaact ttaagaaacg tgtcttcatc attctgaaca taaaaaaaga gaagacctat
    16921 caggtcctct ctagatgtta gcttttcgtc acccactaca gttgacaaac atactaagat
    16981 tagtagtgaa gaaatctccg acgggagaga gtactcacta ctttttc
//
