LOCUS       NZ_JAQMJT010000006     16369 bp    DNA     linear   CON 28-FEB-2024
DEFINITION  Streptococcus salivarius strain 1001095st1_G4_1001095IJ_161003
            NODE_6_length_118876_cov_18.2303, whole genome shotgun sequence.
ACCESSION   NZ_JAQMJT010000006
VERSION     NZ_JAQMJT010000006.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus salivarius
  ORGANISM  Streptococcus salivarius
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 118876)
  AUTHORS   Chen-Liaw,A.
  TITLE     Human gut microbiome strain richness
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 118876)
  AUTHORS   Chen-Liaw,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2023) Precision Immunology Institute, Icahn School
            of Medicine, 1470 Madison Ave, New York, NY 10029, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.10.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 26.073790043805616x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/28/2024 01:16:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,217
            CDSs (total)                      :: 2,158
            Genes (coding)                    :: 2,099
            CDSs (with protein)               :: 2,099
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 19 of 59
            Pseudo Genes (incomplete)         :: 40 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 13 of 59
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:19:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 56919
            Orig. end                         :: 73288
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAQMJT010000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..16369
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="phosphonate"
                     /region_number="3"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     cand_cluster    1..16369
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..16369
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:11369](+)"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..11369
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     misc_feature    263..279
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.57"
                     /tool="antismash"
     gene            424..1317
                     /locus_tag="PNU26_RS06700"
                     /old_locus_tag="PNU26_06700"
     CDS             424..1317
                     /NRPS_PKS="Domain: Aminotran_4 (48-267). E-value: 1.3e-44.
                     Score: 144.6. Matches aSDomain:
                     nrpspksdomains_PNU26_RS06700_Aminotran_4.1"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368611.1"
                     /locus_tag="PNU26_RS06700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06700"
                     /product="aminotransferase class IV"
                     /protein_id="WP_195917981.1"
                     /transl_table=11
                     /translation="MDNGIISIDGALVNNKNIEVNISSASIRYGISVFEAPKMFSLGSD
                     IYIFRFQDYFNRLKTSCKFLELDLPLDSNKLLYDIKKFIEFVPWQESYALRINVFCPYE
                     SELLGEVECALTLSYLDIGIRSKSGVSSKVIKRSNLIRTSNNNLYMIKSPSHYINARKE
                     LYSDIEFDDILYVNEKENICELSRSNIFLIKDRTVYTPDLGSGILDGITRKLIIDLCQE
                     HNILIEIKELNYSNLSDFDSAFCVGTTNGITPIRNIDKNIHFDTNNLLLAEIVNYYKEV
                     FSKKGVEKYKEWFVKL"
     aSDomain        568..1224
                     /aSDomain="Aminotran_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PNU26_RS06700_Aminotran_4.1"
                     /evalue="1.30E-44"
                     /label="PNU26_RS06700_Aminotran_4.1"
                     /locus_tag="PNU26_RS06700"
                     /protein_end="267"
                     /protein_start="48"
                     /score="144.6"
                     /tool="antismash"
                     /translation="FRFQDYFNRLKTSCKFLELDLPLDSNKLLYDIKKFIEFVPWQESY
                     ALRINVFCPYESELLGEVECALTLSYLDIGIRSKSGVSSKVIKRSNLIRTSNNNLYMIK
                     SPSHYINARKELYSDIEFDDILYVNEKENICELSRSNIFLIKDRTVYTPDLGSGILDGI
                     TRKLIIDLCQEHNILIEIKELNYSNLSDFDSAFCVGTTNGITPIRNIDKNIHFDTN"
     gene            1314..2126
                     /locus_tag="PNU26_RS06705"
                     /old_locus_tag="PNU26_06705"
     CDS             1314..2126
                     /GO_function="GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA]"
                     /GO_process="GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DXP_synthase_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1204: transketolase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588707.1"
                     /locus_tag="PNU26_RS06705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06705"
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="WP_195917982.1"
                     /sec_met_domain="DXP_synthase_N (E-value: 5.2e-11,
                     bitscore: 35.0, seeds: 174, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKDVNYFNERAQIIRRTIIDMIFGAESGHFGGSLSSVDFINVIY
                     ENYVFPENAEFILSKGHAAPALYAKLIESGVLDKDFIYGFREYRSLLTGHPNHRIPTLK
                     FGLGSLGQGPSIGVGMAWVNKRKKSDKKIFVMLGDGELNEGQVWEAFYTCRNLNLQNLV
                     FIIDRNFLQLDGKCEDVANFPSLAQKISSFLGTNPIEVNGNSYDEILNVLDNIDYSQTN
                     VIISNTTKGKGIEFMEGKTEFHSYNILSSEKEVLYKRAMECLGGDKMA"
     gene            2123..3034
                     /locus_tag="PNU26_RS06710"
                     /old_locus_tag="PNU26_06710"
     CDS             2123..3034
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232538.1"
                     /locus_tag="PNU26_RS06710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06710"
                     /product="serine hydroxymethyltransferase"
                     /protein_id="WP_195917983.1"
                     /transl_table=11
                     /translation="MRKTFIEELINKNIEEQNIVVLTGDVGRSTYASKFKEIFPENYLN
                     LGICEANIVGISAGIAGTLEFVPFVMLFSKHLILRALEQINDSILMNKKKVILVGGYSG
                     YSASKEGETHQLLNDISILSSFPDISIYCPYDQSSIQTAINESINNDYSSYIRINKNKI
                     LNDVVSRYISQGNKSIIISMGYLGSLLSKKYMEDSDIREFADFILLSKIKPIDWEYWEI
                     FLRKYENAYIIEENTLTGGLGEQFKNYFFGLGINIISFGIKPHFGETGDYETLLTNEGL
                     SPDKIFEKIRRMNHVQSSEKSI"
     gene            3018..4214
                     /locus_tag="PNU26_RS06715"
                     /old_locus_tag="PNU26_06715"
     CDS             3018..4214
                     /GO_function="GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (10-336). E-value: 2.1e-65.
                     Score: 212.9. Matches aSDomain:
                     nrpspksdomains_PNU26_RS06715_Aminotran_3.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282560.1"
                     /locus_tag="PNU26_RS06715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06715"
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="WP_272127856.1"
                     /sec_met_domain="Aminotran_3 (E-value: 4.4e-66, bitscore:
                     216.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRVFEDDNFVKSDGIYLYTDDNKEIIDGCSNSMNVNLGYGVLEI
                     EKVIQEQIRKINFMHTGKGTTYESLMLANRLHDIIKPYGNYKVYFATSGSDCIEAALRI
                     SVLYQQKNKRNQGSNTRFATFEGSYHGSTLGALSVSGHRKFQKLYNGYIGNCLTIPTRW
                     NLEWEMDFTDITAFVLDSMITNPIGSEMIDRDYLNYLVEICKESGVITIIDEIATSIGR
                     LGCFFGFEDSGISPDIVCISKGLGVGYANIGAVIVKSNIIDSINSADILGHTYNASPID
                     CKIAMTVLDYIEEHGIFEHVNNLSNYIEYRLKDLKNKLPCISKVSGKGFMWSIHFRNNV
                     NASKVFKECYNNGLLLLYLEYEAYNHMTFCPPLITSKNQIDSMLDILEKSIMKVLHGH"
     aSDomain        3048..4025
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PNU26_RS06715_Aminotran_3.1"
                     /evalue="2.10E-65"
                     /label="PNU26_RS06715_Aminotran_3.1"
                     /locus_tag="PNU26_RS06715"
                     /protein_end="336"
                     /protein_start="10"
                     /score="212.9"
                     /tool="antismash"
                     /translation="VKSDGIYLYTDDNKEIIDGCSNSMNVNLGYGVLEIEKVIQEQIRK
                     INFMHTGKGTTYESLMLANRLHDIIKPYGNYKVYFATSGSDCIEAALRISVLYQQKNKR
                     NQGSNTRFATFEGSYHGSTLGALSVSGHRKFQKLYNGYIGNCLTIPTRWNLEWEMDFTD
                     ITAFVLDSMITNPIGSEMIDRDYLNYLVEICKESGVITIIDEIATSIGRLGCFFGFEDS
                     GISPDIVCISKGLGVGYANIGAVIVKSNIIDSINSADILGHTYNASPIDCKIAMTVLDY
                     IEEHGIFEHVNNLSNYIEYRLKDLKNKLPCISKVSGKGFMWSIHF"
     gene            4204..4998
                     /locus_tag="PNU26_RS06720"
                     /old_locus_tag="PNU26_06720"
     CDS             4204..4998
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351847.1"
                     /locus_tag="PNU26_RS06720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06720"
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="WP_195917985.1"
                     /transl_table=11
                     /translation="MDIKKLGFIIITNETFAFNLVEEVVEKLSILDVNRAIIKLLPKVD
                     ETTLIKHYREHLINWNGKEGKSQVPSLGWPAVRNRFSSSVVLILLEGNKQSLSDEILQI
                     KGNTYPEQCRQNQIRIKGFNPTYNLMHSSDSAEEALKEAELYFSKKELESFFCGEIGIT
                     FEEILNFILELDSIQKGGIRSNSDLENKVNKMCYWKQRIFDLFSSKTNEESRKISEELN
                     FIKLNRSPEDVILYLENESILWSKLERDLYLSHLIIEEISGE"
     gene            5001..6185
                     /locus_tag="PNU26_RS06725"
                     /old_locus_tag="PNU26_06725"
     CDS             5001..6185
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: HMGL-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1271: 2-isopropylmalate synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239146.1"
                     /locus_tag="PNU26_RS06725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06725"
                     /product="histone-lysine N-methyltransferase"
                     /protein_id="WP_195917986.1"
                     /sec_met_domain="HMGL-like (E-value: 1.1e-45, bitscore:
                     149.1, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVKIYEETLRDGIQSSELSMLNIEDKKKVIKNFDNAGIEGCCIGF
                     INSSKKENDEINELFQFIITNKLKIVPAVLCRLLIEDFKEIKNIVQCNLFNQLKVYTYI
                     AVSPIRMKVENWSEEIIIEKIIDFLNYCQMENTKVTIAFEDASRADKSFLKKLIEIINN
                     YPLVESVVIADTVGTCNYNSTRDLVYFFRKNLFLSKTIEWHGHNDLGLAVSNALSAIES
                     GADIVHTCTLGIGERCGNTSTEQLIINLIVSECGRRATYEPRYLYDVAKILENKSQFPI
                     SPKTPCFGMDSFFTCAGTHASSLYKSYLKNDNNFSIMFSPYDSNIFGREVTVGINSQSG
                     RSSIEYLSGKYGLELSQENITSILGLVKQNNLFFREEEFVDYVKQKRVERLDYK"
     gene            6145..7506
                     /locus_tag="PNU26_RS06730"
                     /old_locus_tag="PNU26_06730"
     CDS             6145..7506
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932706.1"
                     /locus_tag="PNU26_RS06730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06730"
                     /product="hypothetical protein"
                     /protein_id="WP_195917987.1"
                     /transl_table=11
                     /translation="MLNKKEWKDLTISKSENDSIISCLYNWGFELPKSKEYTFSLTWYG
                     YNNVSYKYCEKECLTLGELSLLFPQLNDLFWEHQKDNIEGRRNFYKNCKNLLGKFNIYS
                     VRDILEISDRDLQFALTNRVGSKEFENSIVSLMYINKLSDDNKAENTLEKKLYFSTNNE
                     DSIIPYYENTLGMTAENYYFTASHRTFFSSTFPDFFKIDVPYKISNSVRNLTRNELRIR
                     ASEIIKSELANSGNPILKIFDDYAYVRIGKRNMVLRNKFFNGFIAPLFAFFQQRIFDNG
                     KSFFTHFYDLRKNTNQNIPDYLLLNILFPLIDCFEFFLNVSIKYKRLIFPYDFHMQNIM
                     ISIYDNQIGFIFQDFDMSKELTVTTFYEQLDFFFEKFVVTNILESINELKLDEKKYIMR
                     EMKKIINTKIIIKAESYFGFPSPSEVIFPDFMKQYLRENQNVKERTIKYRSYLR"
     gene            7565..8440
                     /locus_tag="PNU26_RS06735"
                     /old_locus_tag="PNU26_06735"
     CDS             7565..8440
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231: carboxyvinyl-carboxyphosphonate"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809188.1"
                     /locus_tag="PNU26_RS06735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06735"
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="WP_230310570.1"
                     /sec_met_domain="PEP_mutase (E-value: 7.3e-45, bitscore:
                     146.1, seeds: 69, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 2e-72, bitscore:
                     236.4, seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFIDILKKRKMKIIEAHDALSAYIIDQTKYSADKGDIFEYDGIWI
                     SSLCDSIIRGKPDMEVVNITDRLQTIDDILCVTEKHIIVDIDSGGTLEQTASLIEKITL
                     RKIAGIVIEDKIGQKHNSLFGEKNTQLQDSTESFERKIRVAIKSRKNSKTAIIARIESL
                     VLGKSLEDLLYRGNCYIKAGAEALVIHALNSRLDDLYQALKYFKRYFPEIPLIVIPTDF
                     PYVCAEELFERGADLIIYANHLLRSIIKPMEYIAKSILIDGHSNGVENKLLPISEILNY
                     IPLLGEIDEK"
     gene            8430..9527
                     /locus_tag="PNU26_RS06740"
                     /old_locus_tag="PNU26_06740"
     CDS             8430..9527
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055: pyruvate oxidase/decarboxylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836759.1"
                     /locus_tag="PNU26_RS06740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06740"
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="WP_195917988.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 1.9e-12, bitscore:
                     40.0, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 8.7e-14, bitscore:
                     44.4, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSKDLISSLKLNGVTRFFGVPDTFLYPITSQLANDELVIVPNEG
                     NAVSMAFGYSITTSSMPVIFLQNSGLGNILDPVQSLVGQAVYNHPMLYIIGFRGGTDDA
                     PQHSECGKVTKKLLEISQFDIYEKDYFTNALDTDIRRIIDNIKKKNKSAAILVDKEFFS
                     DIVNIKNYQISDYNDILNKICAVLEKEKDSIVLTSTGYITRHMGNIKDTYKKNFVHIPI
                     PGSLGQTISFGAGICMGTSINNKKKKIYIIDGDGSLFMHMGSLALFDYYNLDVTYILLN
                     NYKHLSVGGENTLASSCNFFKLAESMNFDNIYSSDNLEVVDFEEKLNQQGKNFIEIICS
                     NEVTYSLLERMDFNFEEIKQFNMEN"
     gene            9529..10323
                     /locus_tag="PNU26_RS06745"
                     /old_locus_tag="PNU26_06745"
     CDS             9529..10323
                     /GO_function="GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /GO_function="GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809189.1"
                     /locus_tag="PNU26_RS06745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06745"
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="WP_195917989.1"
                     /transl_table=11
                     /translation="MPDRYVEYSQNDYDNIMKLDTLFISQLDSKYNVEYHKLPSLISGE
                     VLKRCGYFSTMPNQLSKVDIIDVSQLESLGNNRELEEIKYSSSENYFLTPAACLHFYPM
                     LEHKEVKECIYSSLVDVFRYENGNFTVGTRQWEFTVREFLAIGNPKFVEEFLEDLKEKF
                     LAIALRFDSTAKIQNACDNFYPTNLNKVKQKFQKYNNLKFELIVHISGKEVSVASFNYH
                     NNHFSKEFNFDSNDTIVTGCVGLGIDRWISLLKESEKDYKLL"
     misc_feature    9549..9569
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: medium, score: 27.92"
                     /tool="antismash"
     gene            10320..11369
                     /locus_tag="PNU26_RS06750"
                     /old_locus_tag="PNU26_06750"
     CDS             10320..11369
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_5"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231246.1"
                     /locus_tag="PNU26_RS06750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06750"
                     /product="nucleotidyltransferase family protein"
                     /protein_id="WP_195917990.1"
                     /sec_met_domain="NTP_transf_5 (E-value: 3e-29, bitscore:
                     95.1, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKQIISLFRAYLNENTQDWVEIDEYFLLYQHLDMFYYYLCKKNN
                     IEPPLMEKFEVRKKVIESRNRQYIKVARDLNAIFENQSIQVAFLKGIQTSEKYYEEPWI
                     RYYSDLDILVAREMIPEVEKLFYQLGYVFGHLKDNGEIHHATREEILYQKLFTHEIYNL
                     VKKENDNVFINVDINFLFSWKGLSDSEIEFNDIKNDIIFDSKIKINTLDKVMNFIHLCC
                     HLYNEARYFALNRSFLGGDPREIQLSRVFEIALILKDLKQEEFNSVVYSSRKLNCDNKV
                     FASIGVTKQLLELNLSILDNYTEELKVKDEFNSYIDKDQKVKYWPISIEDRVFDLKLKV
                     KTCDKIFIN"
     gene            11496..11735
                     /locus_tag="PNU26_RS06755"
                     /old_locus_tag="PNU26_06755"
     CDS             11496..11735
                     /NRPS_PKS="Domain: PP-binding (6-71). E-value: 2.7e-11.
                     Score: 35.6. Matches aSDomain:
                     nrpspksdomains_PNU26_RS06755_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872900.1"
                     /locus_tag="PNU26_RS06755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06755"
                     /product="phosphopantetheine-binding protein"
                     /protein_id="WP_195917991.1"
                     /sec_met_domain="PP-binding (E-value: 2.9e-12, bitscore:
                     39.8, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYDSEIKNVIKDYIDFEFDINEIDNNVALIDYGVDSLKYISIIL
                     ALEDYFKIEIPDNYLVFSKSNTIKKLNSIIEELL"
     aSDomain        11514..11708
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PNU26_RS06755_PP-binding.1"
                     /evalue="2.70E-11"
                     /label="PNU26_RS06755_PP-binding.1"
                     /locus_tag="PNU26_RS06755"
                     /protein_end="71"
                     /protein_start="6"
                     /score="35.6"
                     /tool="antismash"
                     /translation="IKNVIKDYIDFEFDINEIDNNVALIDYGVDSLKYISIILALEDYF
                     KIEIPDNYLVFSKSNTIKKL"
     gene            11809..13338
                     /locus_tag="PNU26_RS06760"
                     /old_locus_tag="PNU26_06760"
     CDS             11809..13338
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875205.1"
                     /locus_tag="PNU26_RS06760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06760"
                     /product="hypothetical protein"
                     /protein_id="WP_195917992.1"
                     /transl_table=11
                     /translation="MKYINSSLKSVDNYYSLEEVTCFDRLLGIFLNSINNSYCDLFYMI
                     NNFYRCYKVDDSKNEYDFEREMHILRNFFCIESEKINFSDEDVLTEFIVKNINKYGSVF
                     VPINLKEIYYSTYYKEQDWPHLFLINGYDKEKELFYVIDATQIYSDILTEQNFCITFEI
                     LERAYKSYFHQTILNKEKEYIFVVTTVENELTEIEIFHETFKYLFNQSSISSREFEIVY
                     KILTNRDFTLLANLKNITKKKKLFFTIFFEKLRVYELISNKELLYLSRTVETILEEWTI
                     FINRCIKNILKNETKLVNYDFFVPEEKKIFDFFSKQASRYKERLFEIISSNSNRYNEVF
                     ECIQNSGKIITITEDNSRKFRFSFIGDKIYNSWFNDDSPKVRINQDLNYLIVKINVIHK
                     EKDSKFVAGLYCFIDDNLYYFGLDSNYFINLDLMGKNPEIFRKRLETSEVYLKITRQED
                     SCKFSYSVDGITYFYATSLDIRSCHFSCGIGCKTYSKPTPLCIDFEDLLIG"
     gene            13746..14738
                     /locus_tag="PNU26_RS06765"
                     /old_locus_tag="PNU26_06765"
     CDS             13746..14738
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /locus_tag="PNU26_RS06765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06765"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_001240822.1"
                     /transl_table=11
                     /translation="MAMIEVSCLRKDFVKIIKEPGLKGAIRSFIHPEKQIFEAVKDLSF
                     EVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQENRQDYVKDIGVVFG
                     QRTQLWWDLALQETYSVLKEIYDVPDAVFQKRMDFLNDVLDLKEFIKDPVRTLSLGQRM
                     RADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLSDIEQ
                     LCDRIFMIDKGQEIFDGTVNQLKETFGKMKTLTFELRPGQNHVVSQFVGISDIHVTRKE
                     LLLNIQYDSSRYQTADIIQKTLSDFAIRDLKMTDVNIEDIIRRFYRKEL"
     gene            14740..15558
                     /locus_tag="PNU26_RS06770"
                     /old_locus_tag="PNU26_06770"
     CDS             14740..15558
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /locus_tag="PNU26_RS06770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06770"
                     /product="ABC-2 family transporter protein"
                     /protein_id="WP_195917993.1"
                     /transl_table=11
                     /translation="MVKLWKRYKLFISAGMQELITYRVNFFLYRIGDVMGAFVAFYLWR
                     AVFSSSHQSLIQGFSLSDMTFYIIMSFVTNLLTKSDSSFMIGEEVKDGSIIMRLLRPVH
                     FAASYLFTEIGFRWLVFVSVGLPFLIIITGLKLSSGQPILQISLMTLTYLLSLILAFLI
                     NFFFNICFGFSAFVFRNLWGSNLLKNSLVAFMSGSLIPLSFFPKIIADILSFLPFSSLI
                     YTPVMIIVGKYNISQMIQAVLLQLFWLLVMIALSQIIWKRVQSHITIQGG"
     gene            15560..16345
                     /locus_tag="PNU26_RS06775"
                     /old_locus_tag="PNU26_06775"
     CDS             15560..16345
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /locus_tag="PNU26_RS06775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06775"
                     /product="ABC transporter permease"
                     /protein_id="WP_195917994.1"
                     /transl_table=11
                     /translation="MKKYQRMHLIFIRQYLKQIVEYKGDFVVGVLGVFLTQGLNLLFLN
                     IIFQNIPSLDGWSFQQIAFIYGFSLIPKGIDHLFFDNLWALGQRLVRKGEFDKYLTRPI
                     NPLFHILVETFQIDALGELLVGISLLSVTVTSLTWTWSKVFIFLISIPFATLIYTSLKI
                     ATASIAFWTKQSGAVIYIFYMFNDFAKYPVVIYNSFLRWLISFIIPFAFTAYYPANYFL
                     KDKDGLFNIGGLILISLIFFTISLKLWKKGLDAYESAGS"
ORIGIN
        1 cagtcacaga aattatggat acttattgtg attggggagt taagtatcca ttagtttatc
       61 ttgaagatct attttccgat gaagatttgg atagttggag aaaatttcaa tcgataaaac
      121 ctctaaagtt acaagttttt ggtgatgatt tttacgcaac aaacttagaa agaatttcac
      181 agtttaaaga ttgtgctgat ggtattgtaa taaagcctaa tcaagttggc tcagtgagta
      241 aaacacttga ggtgatggaa tatgcggaaa aatcagggat tagtatggct ttttcacaac
      301 gcactgctga gaccgagaac aatattatat cacatttagc aatgagtgtg acttcttctt
      361 atttaaaagc tggaggtctg gacaggttgg atagaattgc aaaatataat gaggtattaa
      421 gaaatggata atgggataat cagtatagat ggggcactcg ttaataacaa aaatatagag
      481 gttaatataa gctcagcttc cattagatat ggaattagtg tctttgaggc acccaaaatg
      541 ttctcacttg gttcagatat ctatattttt cgatttcagg attattttaa tcgtttgaaa
      601 actagttgca agtttttaga actagactta cctttggatt ctaataaact tttatatgac
      661 ataaaaaaat ttattgaatt cgtaccttgg caggaatcgt atgctttgag aataaatgtt
      721 ttttgtccct atgaaagtga gctgttaggt gaagttgaat gtgctttgac attatcttat
      781 ttagatattg gcatacgaag caaatcaggt gttagttcaa aagtaataaa gaggtcaaat
      841 ttaattagaa cctctaataa taatctttat atgataaagt ctccatcaca ttatataaat
      901 gcgagaaaag aattgtactc agatattgaa ttcgatgata ttttatatgt aaacgaaaaa
      961 gaaaatattt gtgagctgag tcgttcaaat attttcttaa taaaagatag aactgtatat
     1021 actccagatt taggaagtgg tatacttgat ggcatcactc gaaagttaat aattgattta
     1081 tgccaagaac ataatatttt gattgagata aaagaactta attatagcaa tttatcagat
     1141 tttgattctg ctttctgtgt aggcacaaca aatgggatta ctcctattag aaatattgat
     1201 aaaaatatac attttgatac aaataatttg ctattggctg agatagtaaa ttattataag
     1261 gaagtatttt ctaaaaaagg ggtagaaaaa tataaagaat ggtttgttaa attatgataa
     1321 aggatgtaaa ttattttaat gaaagagctc aaatcatcag gcgaactatt attgatatga
     1381 tttttggggc tgaatctggt cattttggcg gatcgttgtc tagtgttgat tttattaatg
     1441 tcatttatga aaattatgtt tttccagaaa atgccgaatt tatattatcg aaagggcatg
     1501 cagctccagc tttatatgca aaattaattg aatctggggt gttagataaa gattttattt
     1561 atgggtttag agaatatcga agtttattaa ccgggcatcc aaatcataga attccaactc
     1621 ttaaatttgg tttaggaagt ttaggacaag gtccttctat aggagtaggg atggcttggg
     1681 taaataaaag aaagaaatct gataaaaaaa tttttgttat gttaggtgat ggcgaattaa
     1741 atgaaggaca agtatgggaa gcattctata cttgcagaaa tttgaatctt caaaatttag
     1801 ttttcattat tgataggaac tttcttcaat tggatggtaa atgtgaagat gtcgctaatt
     1861 ttccgagttt ggctcaaaag attagttctt ttctaggaac taatcctatt gaagtaaacg
     1921 gtaattctta tgatgaaata ttaaacgtgc ttgataatat agattattcc cagactaatg
     1981 tgattatttc taacactaca aaaggaaagg gaatagaatt tatggaaggg aagacggaat
     2041 ttcattcata taacatttta tcgagtgaaa aggaagtatt atacaagaga gcaatggaat
     2101 gtttaggagg agataaaatg gcatgagaaa aacatttatt gaagagttaa taaataaaaa
     2161 tattgaagaa cagaatatag tagtattaac aggtgatgtt ggaagatcaa cgtatgctag
     2221 taagtttaaa gaaatttttc ctgaaaatta tttaaattta ggtatctgtg aagctaatat
     2281 tgtaggaata agtgcaggaa tagcaggaac tttagaattt gttccatttg taatgctatt
     2341 tagtaaacat ttgattctaa gagcacttga acaaatcaat gatagtatac taatgaacaa
     2401 gaaaaaagtg attttagtag gaggctattc aggttactca gcttcaaaag aaggagagac
     2461 acaccagtta ttaaatgata tttcgatatt gagttcattt ccggacatat caatttactg
     2521 tccatatgat cagtcctcga ttcaaactgc tattaacgaa agcataaata atgattattc
     2581 ctcctatatt agaattaata aaaataaaat tttaaatgat gtagtcagtc gctatatcag
     2641 tcaaggaaat aaaagcataa ttatctcaat gggctatctc ggatcgctac tctcaaaaaa
     2701 atacatggaa gattcagata ttagggagtt tgctgatttt attttattaa gtaagatcaa
     2761 acctattgat tgggaatatt gggaaatttt tttacgcaaa tatgaaaacg catacataat
     2821 tgaagagaat acccttacag gtgggttagg agaacaattt aaaaattatt tttttggact
     2881 aggaataaac atcatttctt tcggtattaa acctcacttt ggagaaactg gtgattatga
     2941 aaccttactt acaaacgaag ggctttcccc tgataagatt tttgagaaaa ttagaaggat
     3001 gaatcatgtg caatctagtg aaaagagtat ttgaagatga taatttcgtt aagtcagatg
     3061 ggatttatct atacactgat gataataagg agataataga tggttgttct aattcaatga
     3121 atgtcaatct gggttatggt gtgttagaga ttgaaaaggt gattcaggaa caaatacgga
     3181 aaattaattt tatgcataca ggcaaaggaa caacctatga atcattgatg ttagctaata
     3241 gattgcatga cataataaaa ccttacggta actataaagt gtattttgca acttctggtt
     3301 cggattgtat tgaagctgct ctcagaatat cagtgttata tcaacaaaaa aataaaagaa
     3361 atcaaggatc caatactaga tttgccacat ttgaaggaag ttatcacggt tctacacttg
     3421 gtgcgttaag tgtatctggt catagaaaat ttcaaaaatt atataatggc tatattggta
     3481 attgtttaac tattcctacg cgatggaact tggaatggga aatggatttt acagatatta
     3541 ctgcgtttgt attggatagt atgattacga atccaattgg ctctgagatg attgatagag
     3601 actatctaaa ttatttggtg gaaatatgta aagagtctgg cgttataaca attattgatg
     3661 aaattgctac atcaattgga cgacttggtt gcttttttgg attcgaggat agtggaattt
     3721 cgccagatat agtttgtata tctaaaggtt taggagttgg gtatgcgaat ataggtgcag
     3781 taattgtcaa aagtaacatt attgattcta ttaattctgc agatattctt ggtcatacat
     3841 ataatgcatc gccaatagat tgtaaaatag ccatgactgt tttagattac atagaggaac
     3901 atggtatatt tgaacatgtg aataacttat ctaattatat agaatataga ttaaaggatc
     3961 taaaaaataa actaccatgc attagtaaag ttagtggaaa aggctttatg tggtctatcc
     4021 atttccgcaa taatgtaaat gctagtaaag tatttaaaga atgttataat aatggtcttt
     4081 tgcttcttta tttggagtat gaagcatata atcatatgac tttttgtcct cccttaataa
     4141 caagtaaaaa tcaaatagac tctatgttag atatattaga aaaatcaatt atgaaggtac
     4201 tacatggaca ttaaaaaatt aggatttatc ataattacaa atgaaacctt tgcgtttaat
     4261 ttagtagaag aagttgtgga aaaattgtca atactggatg taaatcgagc catcataaaa
     4321 ttattgccaa aagtagatga aactactcta attaagcact atagagaaca tctaattaat
     4381 tggaatggaa aggaaggaaa atctcaagtt ccttctttag gttggccagc cgttaggaat
     4441 agattttctt catcagttgt tttaatttta ttggaaggaa acaagcaaag tttatctgat
     4501 gagatactcc aaataaaggg gaacacttat cctgagcaat gccgacaaaa tcagattagg
     4561 attaaagggt ttaatccaac atataatctt atgcattctt cagacagtgc agaggaagct
     4621 cttaaggagg cagaactata tttttcaaag aaagaacttg aaagtttctt ttgtggagaa
     4681 attggtatta cttttgaaga aattctaaac tttattttag agcttgattc aattcaaaaa
     4741 ggaggtatac gatctaatag tgatttagag aataaagtaa ataaaatgtg ttattggaaa
     4801 caaagaattt ttgatttgtt tagttctaaa acgaatgaag aaagtagaaa aatatctgaa
     4861 gagcttaatt ttataaaatt aaatcgttcc cctgaagatg ttattttata tttagaaaat
     4921 gaatctattt tatggagtaa gctggaaaga gatttatact tatctcattt aataattgaa
     4981 gaaatctctg gagaataaat atggtaaaaa tttatgaaga aactttacga gatggtattc
     5041 agagtagcga actttcaatg ttgaatattg aggataaaaa gaaagttatt aagaattttg
     5101 ataatgctgg catagaagga tgttgtattg gttttataaa ttcctcaaaa aaagaaaatg
     5161 atgaaattaa tgaattgttc caatttatta ttactaataa gctgaagata gttccagcag
     5221 ttttatgtag gttactgatt gaggatttta aagaaattaa aaatatcgtt caatgcaatt
     5281 tatttaatca acttaaggtg tacacgtata ttgctgtcag cccaataagg atgaaagtgg
     5341 agaattggag tgaagaaatt atcattgaga aaataattga ctttcttaat tattgccaaa
     5401 tggaaaatac taaagtaact attgcatttg aagatgcttc tagagctgat aaaagctttt
     5461 taaagaagtt aattgaaata ataaacaatt atcctttggt tgagagtgtc gttattgctg
     5521 atactgtcgg aacttgtaat tataattcaa ctagagatct ggtatatttc tttagaaaaa
     5581 atttattttt gagtaaaact attgagtggc atggtcataa tgatttagga cttgctgtct
     5641 caaacgcctt atctgctatt gaatcgggtg cagatatagt acatacttgc actctaggga
     5701 ttggagaaag atgtggaaat acttcaacag agcaactgat aattaatctt attgtaagtg
     5761 agtgtgggag aagagcaact tatgaaccga gatatttgta tgatgttgca aagattttag
     5821 aaaataaatc acaatttcca atatcgccta agacgccgtg ttttggaatg gattcctttt
     5881 tcacttgcgc aggaacacat gcatcatcac tttataaatc atatcttaaa aatgataata
     5941 atttttcaat catgttttca ccatatgatt ctaatatttt tggtagggag gtgacagtag
     6001 gtattaattc tcaaagtggg agaagcagta ttgaatattt aagtggaaaa tatggtttag
     6061 aattaagtca ggagaatata acttctatat taggcttagt gaaacaaaat aacttatttt
     6121 ttagggaaga ggaatttgtt gattatgtta aacaaaaaag agtggaaaga cttgactata
     6181 agtaaatccg aaaatgatag tataatttcc tgcttatata attggggatt cgaacttcct
     6241 aagagtaagg aatatacttt ttcgttaact tggtatggat ataataatgt atcgtataaa
     6301 tattgcgaaa aagagtgttt aacattggga gaactttctt tattattccc tcagttaaat
     6361 gatttgtttt gggaacatca aaaagataat atcgaaggaa gaagaaattt ttataaaaat
     6421 tgtaaaaatt tattggggaa attcaatata tattctgtta gggatattct cgaaatatca
     6481 gatagagatt tacagtttgc actcactaat agagttggat caaaagaatt tgagaactca
     6541 atagtttctt taatgtatat taataagttg tctgatgaca ataaagctga aaatactcta
     6601 gaaaaaaaat tgtatttttc aactaataac gaggatagta taataccata ttatgaaaac
     6661 actttgggga tgacagcaga aaattattac tttacagcaa gtcatagaac attttttagt
     6721 agtacatttc cagacttttt taaaattgat gtcccttata aaatatccaa ttctgtaaga
     6781 aaccttacta gaaatgagtt gaggatccga gcatcagaaa tcataaaaag tgaattagct
     6841 aattccggaa atccgatatt gaaaattttt gatgattatg catatgttag aataggtaaa
     6901 cggaatatgg ttctgagaaa taagtttttt aatgggttca tagcaccgtt attcgctttc
     6961 tttcagcaaa gaatttttga taatggaaaa tcatttttta cccattttta cgaccttaga
     7021 aaaaatacga atcaaaatat accagattac ttgttattga acatactttt tcctttaatt
     7081 gattgttttg aattcttttt aaatgtctct attaagtata aacggttaat ttttccatat
     7141 gattttcata tgcaaaatat aatgataagc atttatgata atcaaattgg ttttattttt
     7201 caagattttg atatgtcaaa agaattaacg gttactactt tctatgaaca attagatttt
     7261 ttctttgaaa aatttgttgt aactaatatt ttagagtcaa taaatgaatt aaaattagac
     7321 gaaaaaaaat atataatgcg tgaaatgaaa aaaataataa atacaaaaat tattataaaa
     7381 gctgagtcat attttggatt tccctcacct tcagaagtga tttttcctga ttttatgaaa
     7441 caatatctaa gagaaaatca aaatgtgaaa gaaagaacta ttaaatatcg atcttattta
     7501 cgttgataga taatttacta gtaatgaaat aacaattaga tttgtattaa tatggagaat
     7561 aaaattgttt attgatattt taaaaaagag aaaaatgaaa attattgagg cacatgatgc
     7621 actaagtgcc tatattatag accagactaa atattctgct gataaaggtg atatttttga
     7681 atatgatgga atttggatta gttcgctctg tgattcaata attcgaggga agccagatat
     7741 ggaagtcgta aatataaccg atcgacttca aacaattgat gatattttat gtgttactga
     7801 aaaacatatt atagtagata ttgacagtgg aggtacatta gaacaaacag cttctttgat
     7861 agaaaaaatc acactgagaa aaattgcagg aatagtaata gaagataaga tcggtcaaaa
     7921 gcataattct ttatttggtg agaaaaacac tcaattacag gatagtacag agtcttttga
     7981 aagaaaaata agggttgcaa ttaaatcgag aaaaaatagt aaaacagcaa ttattgctcg
     8041 aattgagagt ctagttcttg gaaaaagttt agaagattta ctatatagag gaaactgtta
     8101 tataaaggct ggtgctgaag cattggtaat tcatgcattg aattccagat tagatgactt
     8161 atatcaagcg cttaagtatt ttaagagata ttttccagaa ataccactta ttgttatccc
     8221 aacagatttt ccatatgttt gtgcagagga attatttgag agaggagcag acttaattat
     8281 ctatgctaac catttgttaa gaagcattat caaaccaatg gaatatattg caaaaagtat
     8341 attgattgat ggtcatagta atggggtaga aaataagtta ttacctataa gtgaaatatt
     8401 gaattatata ccattattgg gggagataga tgaaaagtaa agatttaata agttctttga
     8461 agttaaatgg tgtaactcgt ttttttgggg ttccagatac tttcttgtat cctattactt
     8521 cacaattagc gaatgatgaa ttggtaattg taccaaatga gggcaatgca gtttcaatgg
     8581 cctttggata tagtattaca acaagtagta tgcctgtaat ttttttacaa aattcaggat
     8641 taggaaatat attagatcct gtacaaagtt tagtggggca agcagtttat aatcatccta
     8701 tgttgtatat aattggattt cggggaggaa cagatgatgc tcctcagcat tctgagtgtg
     8761 gtaaggtaac aaagaagctt ttggagataa gtcaatttga tatatatgaa aaagattatt
     8821 ttacaaacgc ccttgatact gatataagaa gaattattga taatatcaaa aaaaagaata
     8881 aaagtgcagc cattctagtg gataaagaat ttttttcaga tattgttaat ataaaaaatt
     8941 accaaatttc tgattataac gatatactta ataaaatatg tgctgttctg gaaaaagaga
     9001 aagactctat tgttctcaca tcaacaggat atataactcg tcatatgggg aatataaaag
     9061 atacttataa aaagaacttt gttcatatac ctattccagg ctcattagga caaacaatat
     9121 ctttcggtgc tggtatatgt atgggaactt ctataaataa taaaaaaaag aaaatatata
     9181 ttatcgatgg agatggttcc ttatttatgc atatgggttc tttagcgtta tttgattatt
     9241 ataatttaga tgttacatat atcttgttaa acaactataa acatctatca gttgggggag
     9301 aaaacacatt agcttcgagc tgtaattttt ttaagttagc tgaatctatg aattttgata
     9361 atatatattc tagtgataat ttagaagtgg ttgattttga ggaaaaatta aatcagcaag
     9421 gaaagaattt tattgaaata atttgttcta atgaagtgac ttattcatta ttagaaagaa
     9481 tggattttaa tttcgaagaa ataaaacaat ttaatatgga gaattaatat gcctgataga
     9541 tacgttgaat atagtcaaaa tgactatgat aatatcatga aattagatac tttatttata
     9601 tcacagttag atagtaaata taatgttgag taccataagt taccctcttt aatcagtgga
     9661 gaagttttaa agcgttgtgg atatttttct acaatgccaa atcagctatc taaagtagat
     9721 ataattgatg tttctcaact agaatctctg ggaaataaca gagaattgga agaaattaaa
     9781 tatagttcat cagaaaatta ttttttgaca ccggcagcct gcttacattt ttatcctatg
     9841 ttagaacata aagaagttaa agaatgtatt tatagttctt tagttgatgt gttccgttat
     9901 gaaaatggta attttacagt gggtactcgt caatgggagt ttacggtaag agaatttctt
     9961 gctattggaa atccgaaatt tgtggaagaa ttcttagaag atttaaaaga aaaattctta
    10021 gctatagcac ttcgatttga ttcaactgcc aaaatccaaa atgcttgtga caatttttat
    10081 ccgacgaatc taaacaaagt taagcaaaaa tttcaaaagt ataataacct aaagtttgaa
    10141 ctaattgtcc atatttcagg aaaggaggta tctgtagctt cgttcaatta tcataacaat
    10201 catttcagta aagaatttaa ttttgatagt aatgatacaa ttgtaacagg atgcgttgga
    10261 ttaggcattg atagatggat aagtttatta aaagagtcag aaaaggatta taagttatta
    10321 tgagaaaaca aatcatatcg ctgtttcgtg cttatcttaa tgaaaatacc caagattggg
    10381 tggaaattga tgaatatttc ttactttacc aacatttaga tatgttttat tattatttat
    10441 gtaaaaagaa taatatagaa cctcctttaa tggaaaaatt tgaagttaga aaaaaagtaa
    10501 tcgaaagcag aaatcgtcag tatataaaag ttgcaagaga cttgaatgct atatttgaaa
    10561 atcaatctat acaagttgct tttttaaaag gaattcaaac ctcagaaaag tattatgaag
    10621 aaccatggat tagatattat agtgatttag atattttggt agctagagaa atgataccag
    10681 aggtagaaaa attattttac caactgggtt atgtttttgg gcatctcaaa gataatggtg
    10741 aaattcatca tgcaactcga gaagaaatac tctatcaaaa actctttaca catgagatat
    10801 ataatttagt gaaaaaagaa aacgataatg tatttattaa tgttgatatt aattttttgt
    10861 tttcttggaa aggtttaagt gattcagaaa ttgaattcaa tgatattaaa aatgatatta
    10921 tctttgatag taaaataaaa attaatactc tagataaggt gatgaatttt atacatcttt
    10981 gttgtcattt atataatgaa gcgagatatt ttgcattgaa tagatctttt ttgggtggag
    11041 atccaaggga aattcagtta agcagagtat ttgaaattgc tttaattctt aaagacttaa
    11101 agcaagaaga gtttaattca gtagtttata gttctagaaa attgaattgt gataataaag
    11161 tttttgcatc aataggtgtg actaaacaat tattggaact caatttatca attttggata
    11221 attatactga agaattaaaa gtgaaagatg agtttaatag ttatattgac aaagatcaga
    11281 aggttaagta ttggcctatt tctattgaag atagagtttt tgatttaaaa ttaaaagtaa
    11341 aaacatgcga taagatattc attaattaat tttatttatg aaccattttt cgatttttgt
    11401 cagtaattaa ggcgaatgtc aaaaatattt ttaatatttt tgcaaccgta tgaaatttag
    11461 tatattaaat taattaaaaa aagagggata aaatattgaa atacgacagc gaaataaaaa
    11521 atgttattaa agattatata gattttgagt ttgatatcaa tgaaatagac aacaatgttg
    11581 ctctaattga ttatggagtt gattcgttaa agtatatttc tattatactt gctttggaag
    11641 attattttaa gattgagatt ccagataact atttagtttt ttcaaaatca aatacgataa
    11701 aaaaattgaa ttctattatt gaagaattgt tgtaatagca gcatatatct agcttaggaa
    11761 actaattttg taattgaata atatatgaat aaagaaaata atattaatat gaaatatata
    11821 aattcttcat taaaatcagt tgataattat tattcattgg aagaagttac gtgttttgat
    11881 agattattgg gaattttctt aaatagtatc aataatagtt attgtgactt attttatatg
    11941 attaacaatt tttatagatg ttataaggtg gacgacagta aaaatgaata cgattttgaa
    12001 agagagatgc atatactaag aaattttttc tgtatagaga gtgaaaaaat taacttttct
    12061 gatgaagatg tgttaacaga attcatcgtc aaaaatatta ataaatatgg ttctgttttt
    12121 gttccaataa atcttaaaga aatttattac tctacttatt acaaagaaca agactggcca
    12181 catctttttt taataaatgg ctatgataag gaaaaggaac tcttttatgt tatagatgct
    12241 acacagatat attcagatat acttacggaa caaaattttt gtattacttt tgaaattctt
    12301 gaacgagcct ataaatcata ttttcatcag actattttaa acaaagaaaa agaatatatt
    12361 tttgttgtta ctacagtaga aaatgaactg accgaaattg agatatttca cgagacattt
    12421 aaatatttgt ttaaccaaag tagtatatca tccagagaat tcgagatagt atataaaata
    12481 ttgacaaata gggattttac attactggct aatttaaaga atattacaaa aaagaagaaa
    12541 ttatttttta caatcttttt tgagaaactt cgagtatatg aattgatttc taataaagaa
    12601 ttactatatc tatctaggac ggtggaaact attcttgagg aatggaccat atttattaat
    12661 agatgtatta aaaatatttt gaagaatgag actaaattag taaattatga tttttttgtt
    12721 ccagaagaaa agaagatttt tgattttttt tccaaacaag cttctaggta taaagaaaga
    12781 ttatttgaaa tcatttcatc aaattcaaat agatacaatg aagtatttga gtgcatacaa
    12841 aatagcggga aaattattac aattactgaa gataactcca gaaaatttag gtttagtttt
    12901 atcggtgata aaatttacaa ttcatggttt aatgatgata gtccaaaggt aagaatcaat
    12961 caagacttaa actatcttat agtgaaaatt aatgtaattc ataaagaaaa agattctaaa
    13021 tttgttgcag gtctatattg ttttatcgat gataatttat attattttgg tttggactca
    13081 aattatttta taaacttaga tttaatggga aaaaatcctg aaatttttag aaagcgactt
    13141 gagacttcgg aagtttattt aaaaataact agacaggagg atagttgtaa attttcctat
    13201 tcagttgatg gaattacata tttttatgca acgagtttag acattcgttc ttgtcatttt
    13261 tcttgtggaa ttggttgcaa aacctactca aagccaacgc cattatgtat agattttgag
    13321 gaccttttaa ttgggtaatt ttttaatggt aattatctag cttttctata acagaaaagc
    13381 tagtacttgt gtattctagg aataagaagt tggggtatac ttagtataaa tattctttta
    13441 tgtacaatta tccattatgg tgaagtttaa aaaaagtgat accttttata acttggctat
    13501 agttctaggt aactttagaa agtaaattta tatccattta gaccaacttt ttactgtttt
    13561 tataactgaa atatgtaaag tacatttaaa aaaattttag tagtttattt tctgtggatg
    13621 taaaacaaca ttgtgaggtt attgttttaa gtgttctaat aattaagatt acaatagctt
    13681 tatggtggac aaaaacttgg attgaacact gattaccata taaatagata aaaggagagt
    13741 aaagtatggc aatgattgaa gtatcttgtc ttcggaaaga ttttgttaag ataattaaag
    13801 aacctggctt aaaaggagcc atacgttctt ttatccatcc cgaaaaacaa atttttgaag
    13861 cggttaagga tttatcgttt gaggttccaa agggacaaat tttaggtttt attggtgcta
    13921 atggggcagg taagtcaaca acgattaaaa tgttgacagg gattttaaaa ccgacatctg
    13981 gtttttgtcg cattaatggg aagattcctc aagaaaatcg tcaggattat gttaaagata
    14041 ttggtgttgt ttttgggcaa cgcacccagc tttggtggga tttggcttta caagagacat
    14101 actcagtttt aaaagaaatt tatgatgtcc ctgatgcagt ctttcaaaaa cgcatggatt
    14161 ttttaaatga tgttttagac ttgaaggagt ttattaagga tcctgttcgg acactatcgt
    14221 taggtcaacg tatgagagct gatattgcgg cttctttatt gcataatccc aaagtccttt
    14281 ttttagatga gcccaccatt ggtttagatg tttctgtcaa ggataatatt cgacgagcga
    14341 tcacacagat taatcaagaa gaagagacga caatcctctt aaccactcat gatttgagtg
    14401 atattgaaca attatgtgat cgtattttta tgattgataa gggacaagag atttttgacg
    14461 gcactgtaaa tcaactgaaa gaaacttttg gaaagatgaa gacccttact tttgaactac
    14521 gtccaggtca aaatcatgtt gtctctcaat ttgtagggat ttcggatatt catgttacgc
    14581 gaaaagagtt gctactaaat attcaatatg atagctcgcg ttatcaaaca gctgatatta
    14641 tccaaaaaac cttgtctgat tttgccattc gagatttaaa gatgaccgat gtaaatattg
    14701 aagatattat ccgtcgtttc taccgaaagg agctttaaga tggttaaatt gtggaaacgc
    14761 tacaaactat ttattagtgc tggaatgcag gaattgatta cttatcgtgt caattttttt
    14821 ctttaccgaa tcggggatgt tatgggagca tttgttgctt tttacttatg gagagccgtt
    14881 ttcagttcgt cgcatcaatc cttgattcaa ggatttagct tatctgatat gactttttat
    14941 atcatcatga gttttgtgac taacttgctg acaaaatcgg atagctcatt tatgattggc
    15001 gaggaagtaa aggacggctc aattattatg cggttgcttc gtccggttca ttttgcagct
    15061 tcttatcttt tcacagagat tggatttagg tggttagtct ttgttagtgt tggcttgccg
    15121 tttttaatca taattacagg cttaaaactc tcatccggac aacctatctt gcaaatttct
    15181 ctaatgacct tgacctatct tcttagctta atattggctt ttttgattaa ttttttcttt
    15241 aatatttgtt ttgggttttc cgcttttgtt tttagaaatt tgtgggggtc aaatcttcta
    15301 aagaattctt tggtcgcctt tatgtctggt agtctgattc ctttgtcttt ttttccaaaa
    15361 atcattgcgg atatattatc ttttttacca ttttcatctt taatttatac gccagtgatg
    15421 attattgtag gaaagtataa cattagtcaa atgattcaag ctgtcttgct acaattattt
    15481 tggttgcttg ttatgattgc tttgtcccaa attatttgga aacgtgtaca gtcccacatt
    15541 actattcaag gaggttagta tgaagaaata tcaacgtatg cacttgattt ttatcagaca
    15601 atacctcaag caaattgtgg agtataaggg tgacttcgtt gttggcgttt taggcgtttt
    15661 tttaacgcaa ggattaaacc tgctattcct taatattata tttcaaaaca tcccatcgct
    15721 tgatgggtgg agttttcagc agattgcctt tatctatggc ttttccctaa ttcccaaggg
    15781 aattgatcat ctcttttttg ataatttatg ggctctgggg caacgcctgg ttcgtaaggg
    15841 cgaatttgat aaatacctga caagacccat taatccttta tttcatattc tagtagaaac
    15901 ttttcaaatt gatgctttag gtgaattgtt agtaggtatt tcattactat cagtaacggt
    15961 aaccagcttg acttggactt ggtcaaaggt ttttatcttt ttaattagta taccttttgc
    16021 caccttgatt tatacttcct tgaaaattgc aacagccagc atagcttttt ggaccaaaca
    16081 atccggggca gtgatttaca ttttttatat gttcaatgat tttgcaaaat atccggttgt
    16141 catctacaat tcatttttac gttggttgat aagttttatt attccttttg cttttacagc
    16201 ctactaccca gctaactatt tcttgaaaga taaggatgga ttgtttaata ttggtggatt
    16261 aatccttatt tcattaatat ttttcaccat atcattaaaa ttatggaaga aaggtttgga
    16321 tgcatatgaa agtgctggat catagctcct aaagatttca atatctata
//
