LOCUS       NZ_JAQMJT010000006     13922 bp    DNA     linear   CON 28-FEB-2024
DEFINITION  Streptococcus salivarius strain 1001095st1_G4_1001095IJ_161003
            NODE_6_length_118876_cov_18.2303, whole genome shotgun sequence.
ACCESSION   NZ_JAQMJT010000006
VERSION     NZ_JAQMJT010000006.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus salivarius
  ORGANISM  Streptococcus salivarius
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 118876)
  AUTHORS   Chen-Liaw,A.
  TITLE     Human gut microbiome strain richness
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 118876)
  AUTHORS   Chen-Liaw,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2023) Precision Immunology Institute, Icahn School
            of Medicine, 1470 Madison Ave, New York, NY 10029, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.10.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 26.073790043805616x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/28/2024 01:16:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,217
            CDSs (total)                      :: 2,158
            Genes (coding)                    :: 2,099
            CDSs (with protein)               :: 2,099
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 19 of 59
            Pseudo Genes (incomplete)         :: 40 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 13 of 59
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:19:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 13922
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAQMJT010000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..13922
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-iv"
                     /region_number="1"
                     /rules="(cds(LANC_like and Pkinase) and not micKC)"
                     /tool="antismash"
     cand_cluster    1..13922
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(cds(LANC_like and Pkinase) and not
                     micKC)"
                     /kind="single"
                     /product="lanthipeptide-class-iv"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13922
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[1735:3922](+)"
                     /cutoff="20000"
                     /detection_rule="(cds(LANC_like and Pkinase) and not
                     micKC)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-iv"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1736..3922
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cds(LANC_like and Pkinase) and not
                     micKC)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-iv"
                     /protocluster_number="1"
     gene            769..1641
                     /locus_tag="PNU26_RS06415"
                     /old_locus_tag="PNU26_06415"
     CDS             769..1641
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR01716"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014633824.1"
                     /locus_tag="PNU26_RS06415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06415"
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="WP_002887152.1"
                     /sec_met_domain="TIGR01716 (E-value: 4.9e-38, bitscore:
                     123.7, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIETTEMGALFKKFRLSKKLALKDVAGDYLSISFLSKFERGESEI
                     SLSRFLLLLENLDVSIEEFYGILSKEKTTYTELLLERVSKAYYQNNILALKKYLKEENK
                     KYHDSNKKLFLYNTIMIESFLSTITEEDVDEDKIKIITDYLFDIEQWGKRELIILGNSM
                     PAISSETLNVLVKEVIYRTTLFGNNESNLKIRIDLLINATSEFIERNQLDLAKSYLDII
                     SDIGIPELFLYEKLEYNILLGAFWIKSGKVEIGKKLIENCLSILKTLGANNLLVSHEAY
                     YEELLNSTI"
     gene            1736..3922
                     /locus_tag="PNU26_RS06420"
                     /old_locus_tag="PNU26_06420"
     CDS             1736..3922
                     /GO_function="GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-iv: LANC_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-iv: Pkinase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681723.1"
                     /locus_tag="PNU26_RS06420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06420"
                     /product="protein kinase"
                     /protein_id="WP_002887151.1"
                     /sec_met_domain="Pkinase (E-value: 8.7e-16, bitscore: 50.8,
                     seeds: 54, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 3.4e-09, bitscore:
                     28.7, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIDTMIFPTNLSPDNLIINSNFTYVFSTNKSIPYQGWKIHISATL
                     DNYQIILDSVTTICLTNDTPFKFINSIEKLFFIFSKKTSQTEIGKFITIYPENDQTFLY
                     LLDVLYQELKYESGIQIMTDQNYKQSQVIFYRYGLMNASVKNGQRPCLFDNNGVAYPDL
                     PGAYYSCPEFVTEYFPEKTNTSTILNERYDMKSILYQTGAGNIYIAIDITNSQKVIIKE
                     ARKYVYATRDITSIELLKNEAKIIKKLSGSVTPNYIDDFYLEGNYYLVEEFIEGKTLNA
                     LKTDFNLLLKRTPSEITTFNNKLQKIISKLFKSLEYIHNHGVIVDDISCSNIILTSENK
                     VYFIDFETAYLKNEKPLIATTNSCYPKTIYEKFEHKDIVKLWYCIINLLTNASSLLKFD
                     QTGTATLKLFTKMCLEMNFPSQLLNKFMKDFKLERNNRMFFINFIKLGMDLEKLIYKRD
                     QLLDTLVTSSTIFIEGKSWCEKVDNYLAINQLGLTPSDKLTVKDIQGSDLDSQLARILL
                     DESQENLSDDMLSENFFNDLEYKEKYRLLTILNSKLKNPNLFRKLVKSIISNNIRYEKN
                     VPYLIIEDYLSPYLVDGNSGLIIELIKFSISEQTTEYDDFIKKLARGVEHPYAKSTSLY
                     NGLAGLGLANAWLYRYFDKMYYLQTSIDIVNHICDYSIEKNGKFILIDPIRDEIKYSYS
                     HGMLGQFYFFETLISLISKVQNKDKNYEKIHLTI"
     gene            3897..5465
                     /locus_tag="PNU26_RS06425"
                     /old_locus_tag="PNU26_06425"
     CDS             3897..5465
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002886453.1"
                     /locus_tag="PNU26_RS06425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06425"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002887150.1"
                     /transl_table=11
                     /translation="MKKYILQFKFKNLLHLFLIACNSAFLVGASVTLAFMTNQLVAANF
                     KSFLIWLGIEILLYLLYLVFTYIITIHQAKLIQEMSLKIRSDYIKNITNSSFHTFQSKT
                     IGDHLSILNNDIQIIENSGFSNLYSLFSTLFTTLFSIIALLSYDIRIVVLTILLTICLT
                     YLPKPFATKMQHFMSLFSTANEELISGLNDQLSGYKTLYYSNKKPTLLIQNTKIIRNYI
                     TQKVNFTQNSTRIETIMSAFSIMAQMSILFLTGLLITLGQVSIGSISSVGQISGNIFNS
                     LTTLNQLQVSIHSVKPLFLKFEVSSKHTEKRTVNNIENIDISDLEYNFGNKTVFSKLNL
                     NLVKNKKYAIIGESGSGKSTLINIILGNLQNYTGHVKYNNLELKEIDENSIVSQVAYIS
                     NSTHIYNDTLENNLTLWSQDITQNEIKKALKNVNLLDLQGRLKEKVSNDLLSEGQKQRI
                     GIARALLKGSKIIIMDEATANLDKTNADFIENNLLKNPDITYITVTHHLSSEREKYFDQ
                     IIKLA"
     misc_feature    5476..5491
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 20.57"
                     /tool="antismash"
     gene            5928..6071
                     /locus_tag="PNU26_RS06430"
                     /old_locus_tag="PNU26_06430"
     CDS             5928..6071
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PNU26_RS06430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="PNU26_06430"
                     /product="hypothetical protein"
                     /protein_id="WP_002887149.1"
                     /transl_table=11
                     /translation="MFMILVLLLTLASFLYVTIGIAKKIFSIFALITPIVVLGVGLLIA
                     MN"
     gene            6159..7490
                     /locus_tag="PNU26_RS06435"
                     /old_locus_tag="PNU26_06435"
     CDS             6159..7490
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887148.1"
                     /locus_tag="PNU26_RS06435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06435"
                     /product="hypothetical protein"
                     /protein_id="WP_195520068.1"
                     /transl_table=11
                     /translation="MKALKLIREDGYENVHYASLGEQKFKTLVVADRKDEQEQWFEQPE
                     IECVPEEVSDIIRNYTLPYIDLGNTEDEYLLEQATNKVVSVVVENVLETQPFIPRNRNG
                     RPIGEGVAFCDMLVGRVQNGELAGTTVYIKQTESGFRQPKDFFKMVGRIFPCKLTEIHI
                     QTIDNYKLTINNPSNYVVEGSIELAEWLTNYQLFQQIEKSKDNSSRNRDRKNILERTHE
                     GIVSAVINNGSNAGIMVMTKKLQTVFIPKHRVSYECDSSFVNLDEVVSVFDRVEFKITG
                     ANTQKPSPNQRERHIIHDYIRIEGDMKVLEEAPQDALIEMIDNKKVIGRLFKVRFISFD
                     PQSLHTVELVDFPGIKIKMKDSGSLNRVPFKQGDEISVVIQDAHYRWVDANSRGRRVAK
                     KTEEVSSNSQNTEEKKCLFRIKSRFNSFYPNRVNSKILSTFFTS"
     gene            7565..8053
                     /locus_tag="PNU26_RS06440"
                     /old_locus_tag="PNU26_06440"
     CDS             7565..8053
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887147.1"
                     /locus_tag="PNU26_RS06440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06440"
                     /product="hypothetical protein"
                     /protein_id="WP_195520069.1"
                     /transl_table=11
                     /translation="MNHHNINAIVYYLIVFIRYVLPITLVFIGIGRWSYAKSINNPEKQ
                     IMGVNFALKSILFLYIVTFIPIFIIYGLAIISKGGTHSGDFSMISFVEVFTLIEILAVG
                     ILPAYGFIQGYIYHYQWLITEGKKTTSLPRRNLFIRLGVISPIAIVVFMEFLKRMFAY"
     gene            8065..11103
                     /locus_tag="PNU26_RS06445"
                     /old_locus_tag="PNU26_06445"
     CDS             8065..11103
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002892502.1"
                     /locus_tag="PNU26_RS06445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06445"
                     /product="hypothetical protein"
                     /protein_id="WP_272127821.1"
                     /transl_table=11
                     /translation="MRKILKLFFNRYYTNGKRTIDILLYCWRKYIDWVYHISFGLFAFN
                     IIMFIFRWRLPNWVFISLFIFLFVIMALVLYIGVWHSNVTSRQIFVLFEKYDTLKRAER
                     KKIIGKYAIRAALAAVLISSGLYVLTINNFILSLINRMYDVADNYLKYMPWSIPNGSQT
                     GIVLSYHTLKPLIGADWFNSLLLLTPIGAIGLIFYNSYATGIAEKTYEKLVEQWLGGRY
                     FQDSVLSNIVHNQDEKGQANLVMGLNSKTGEQVILHANERTYHTIIFGLIGTGKSATIL
                     IPAMLQDMENMKYYLLSYCKYLMKVDEELEQEFFSTEFERKKKRRELLEQWYNNGYAAE
                     LTNGIYMTEPSGSMYGTIVKYAHKMGFPDEMIWTLDPTNPGTGAINILDGDTTQVKGAV
                     RDLFKRFSDPSGTGSSFFLNQEGQLTDNIITVIKEVALIPDAPINYMLKGKSPTLTEFS
                     NMLRSSDWIEAYIAVFEQLLNRKKRLFEETLKKHEEFLEQEKEKWLEESEYNREHEQLF
                     DTILRPENVHEAINAFNDERNKLENLQSSYDYFINAHQLNSYTEEYYYTFDANVSGLKA
                     VIDDLSSSPEIRRVFFSQSTKDLDIVLKTGGMILVNSAKGVLGKEKSRMVAQMVTLVMQ
                     NAAQRRLPDKSPFFAFYEDEKNGYLMPGNDSNFLDESRKFHVPVMHAYQHDEQIRDSIG
                     ESGADAIKMSYRNAFTFQQTSTKVVEFINLRSGGKHYALKETKRAANDDLLAGNQGNSS
                     ATSEEVVEEDNFTARDANNLEKFQVGGVIVSDDEVSNMIYLTTSPHFEMDISKDSFVSH
                     TPFNKVYGLGEEEPEIAKAYKLWRAEVIEYYAQKESESHLYESDFTEEEWQAIMDVKVP
                     NVDSNVNDTVGKKDDSPSVKRTILVNESMTEHRENTSIREEMTEDTEQKVEVDISEIPP
                     VVEDEQTVKSKNDIVELKDKEITQRVILAPSSTPETVDTQNIQAPNQEFELPQELSRVA
                     REKATKTLKSNVEQMAIDEEEGF"
     gene            complement(11141..11449)
                     /locus_tag="PNU26_RS06450"
                     /old_locus_tag="PNU26_06450"
     CDS             complement(11141..11449)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002892500.1"
                     /locus_tag="PNU26_RS06450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06450"
                     /product="hypothetical protein"
                     /protein_id="WP_195918036.1"
                     /transl_table=11
                     /translation="MAGKNKKTESDFIKEIEAYEQQKKDAMEQLKAYQKSQTDKLGQLY
                     FELKKLENPDLSIDDLVVETQNKVKEVKKEIKNKKAAEKARKEAEKTNKETYNDSQN"
     misc_feature    complement(11627..11643)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 21.73"
                     /tool="antismash"
     gene            11990..13258
                     /locus_tag="PNU26_RS06455"
                     /old_locus_tag="PNU26_06455"
     CDS             11990..13258
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PNU26_RS06455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="PNU26_06455"
                     /product="replication-relaxation family protein"
                     /protein_id="WP_230310578.1"
                     /transl_table=11
                     /translation="MQDIKNRDFTSLISNKKNKIHYTQRNKIATYFVSKNQNISLSFSE
                     EELIIFYHLLRFGYFDKEIIDLIYTAVTRKSKTPNSWFYRLVGKVNHPISASSDARSGK
                     RKIYYLNKGFSTWLLTMIMGIDKIRLLTTTTEYDRSVYSIVSNNLLGGKPQTVNIHDLE
                     TRRLVAKILNKLFFNNQDKSVYDLNIQVVFPISRVNLLMVPDAVVFVNDSPFFIEYDRA
                     TERQTILLSKIFGYAEEPAFENSSLFFVFENSRKDNTQVSQRLMNFLEYVNEVEVEGVT
                     GHDMLKRKNISLFSQTTTNASDVIVEIIQEKMFSNHKDWCDCEAYDFSELQKIDYLVKE
                     ISEPLDDDKFQLSAVVETDSWELKEYPMVPMAYIKNDLIPHLTSLYEEFSIEYEHMIIV
                     FPKEVLPEKIKLPFDDYFLIVYR"
     gene            13276..13638
                     /locus_tag="PNU26_RS06460"
                     /old_locus_tag="PNU26_06460"
     CDS             13276..13638
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887142.1"
                     /locus_tag="PNU26_RS06460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_06460"
                     /product="hypothetical protein"
                     /protein_id="WP_195918037.1"
                     /transl_table=11
                     /translation="MDYLEYLGRRLVWLVVSLVVVIVVKRPRVLKRLAIVSDVAIGKIH
                     YFIFLAIAWLILVTYFILYLFMVVTFFLSWHSLSLNNVMEIILQTVLIIPTLYTLFVPH
                     NEIIKRVDEFLSAKKE"
ORIGIN
        1 ttttttatga aaaatttctt ttatgacaaa attactctac ttttttagaa gtttagtaat
       61 actgtttata ttcttcatgc gataacttat ttatcaaata tgaaacttct ccctaattta
      121 gaggtattta gtttaaaatt aatttatcaa atttaattaa aggaaatatc tgcttatgaa
      181 taatatcttt tttggagacg aaattgtagt tctttcattg ctaaaaaata cacacttgcg
      241 aggagaaaaa atatgaatgt acaacaactt aataaacttc ctcaagaaag aatcaccaca
      301 caaggtagac cgattatcac cctctattgc cctatcgcag tataaaggag aaaaaatatg
      361 aatgtacaac aacttaacaa acttcctcaa gaaagaatca ctacacaagg tagaccgatt
      421 atcaccctct attgccctat cgcagtataa aggagaaaaa atatgaatgt acaacaactt
      481 aacaaacttc ctcaagaaag aatcactaca caaggtagac cgattatcac cctctattgc
      541 cctatcgcag tataaaggag aaaaaatatg aatgtacaac aacttaataa acttccttaa
      601 gaaagaatta ccacacaagg tggactgatt atcactctct attgtcctat cgtagtatct
      661 taacagtaag aaggctatgt tcgctaaaca cgctaaacat aggtcttttt actttttttt
      721 ttttttttga tataatctat taaaatttta cctattgaaa gaagtactat gattgaaaca
      781 acagaaatgg gcgcactatt caaaaagttt cgcttatcaa aaaaattagc tttaaaagac
      841 gttgctggtg actacttatc tatatctttc ttgtcaaaat ttgagcgtgg agaatcagaa
      901 atttctttat ctcggttctt attactacta gaaaatctag atgttagtat agaagaattc
      961 tatggtattc tctcaaaaga aaaaacgact tatacagaac tactgttaga acgtgttagt
     1021 aaggcgtatt atcaaaataa tatacttgct ttaaaaaaat acctaaaaga agagaataaa
     1081 aaatatcatg atagcaataa aaaattattc ctgtataata caatcatgat tgaatccttt
     1141 ttatctacaa ttacagagga agacgtagat gaggataaaa taaaaattat cacggattat
     1201 ctttttgata ttgaacaatg gggtaagagg gagttgataa tactaggaaa tagtatgcca
     1261 gctatttctt cagaaactct caatgtactt gtcaaagaag tcatttacag aacaactctt
     1321 tttggaaata atgaatcaaa tttaaaaatt aggattgatt tactgataaa tgcaacatct
     1381 gaatttattg aaagaaatca actagattta gcaaagtcgt atttagatat aatttcagat
     1441 ataggtattc cagaactctt tttatacgaa aaacttgagt ataatatact tttaggagct
     1501 ttctggatta aatcaggtaa agttgaaatt ggaaaaaaac ttattgagaa ctgcctttca
     1561 atcctcaaaa ctttaggagc aaataatcta ctcgtatctc acgaagccta ctatgaagaa
     1621 ctactaaatt ccactatata acaatatcta actttgatgt ttaaaatggt gtaagctaga
     1681 actataaaaa gatattatct ttttatagtt ctagcttttt ttaaggagaa tatttatgat
     1741 tgataccatg atttttccaa ctaatctatc cccagataac ttaattatta actctaactt
     1801 tacctatgta ttttcaacta ataaatctat cccataccaa ggctggaaga tacatatttc
     1861 agcaacatta gataattatc aaataatatt agattctgta actactatct gtttaacgaa
     1921 cgatacccca tttaaattta taaactctat agaaaaatta ttttttatct tttcaaaaaa
     1981 gacatctcaa actgagattg gaaagtttat tactatctac cctgaaaacg atcaaacctt
     2041 tctctatcta ctcgatgtcc tatatcaaga attaaaatat gaatctggca tccaaataat
     2101 gacagaccaa aattataaac agtcacaggt tatattttat aggtatggac tcatgaatgc
     2161 atctgtaaaa aacggccaaa gaccctgtct ttttgataat aatggtgttg catatccaga
     2221 tctaccgggt gcatactatt cttgccctga atttgtaaca gaatactttc ctgaaaaaac
     2281 taatacctct actatcctaa atgagagata cgatatgaaa tccatccttt atcaaactgg
     2341 tgcaggaaat atctatatcg caatcgatat tactaattcc caaaaagtga ttataaaaga
     2401 agctcgaaaa tatgtctatg ctactaggga tattacttct atagaattac taaaaaatga
     2461 agctaaaata attaaaaaat tatcaggttc agtaactcct aattacattg atgattttta
     2521 tttagaaggt aactattacc tcgttgagga atttattgaa ggaaaaacac ttaacgcttt
     2581 aaaaacagac tttaatttac tcctaaaacg aactccttca gaaattacaa ctttcaataa
     2641 taaactccaa aaaattattt caaagctatt taaatcatta gaatatatcc ataatcatgg
     2701 tgttattgtt gacgatatct cctgtagtaa tatcatatta acatcagaaa acaaagttta
     2761 ttttattgac tttgaaacag catatttaaa aaatgaaaaa ccattgatag caacaacaaa
     2821 tagctgctat ccaaaaacaa tatatgaaaa atttgaacat aaagatatag tcaaattatg
     2881 gtactgtatc atcaatctat taaccaatgc aagttcactt ttaaaatttg atcaaactgg
     2941 aacagcgacc ttaaagctat ttacaaagat gtgtttagag atgaacttcc cttcacaact
     3001 attaaataaa tttatgaaag actttaagtt agaaaggaac aatagaatgt tttttattaa
     3061 ctttatcaaa ctaggaatgg atttggaaaa actcatatat aaacgagatc aactgttaga
     3121 tactctagta actagttcta caatttttat agaaggtaaa tcttggtgtg aaaaagtaga
     3181 taactatcta gcaataaatc aattaggact tactccatct gataaattaa cagtaaaaga
     3241 tattcaagga agtgatttag attctcagtt agcaagaatt ttactagatg aatcacaaga
     3301 aaatttatct gacgacatgt tatctgaaaa cttttttaat gatctggaat acaaagaaaa
     3361 atatcgtcta ctaaccattt taaactcaaa actaaaaaat cctaatttat ttaggaagct
     3421 agtaaaatct attatttcta ataacatcag atacgaaaaa aatgttcctt atctaatcat
     3481 tgaagactat ctttctccat atctagtaga tggaaactca ggtttaatta ttgaattaat
     3541 aaaatttagt atttctgaac aaactacaga atatgatgat tttattaaaa agctagctag
     3601 aggagtagaa catccctacg ccaaaagtac atcactctat aatggattag cagggcttgg
     3661 gcttgcaaat gcctggttat atcgctattt tgataaaatg tattatttac agactagtat
     3721 tgatatcgtt aatcacattt gtgattactc aatagaaaaa aatggtaaat ttatattaat
     3781 tgatccaata cgtgatgaaa taaagtatag ctattctcat ggcatgttag gccaattcta
     3841 tttttttgaa actcttatct ctttgataag taaggtacaa aataaggaca aaaattatga
     3901 aaaaatacat cttacaattt aaatttaaaa atttactgca tctatttctt attgcttgta
     3961 attctgcatt tcttgtaggt gcttctgtta ccttagcatt tatgaccaat caattagtcg
     4021 cagctaattt taaaagcttc cttatttggt tgggaattga aatattactt taccttctct
     4081 atcttgtatt tacttatatc attactattc atcaagctaa gttaatacaa gaaatgtctc
     4141 taaaaattcg ttccgattat atcaaaaata taactaattc ttctttccat actttccaaa
     4201 gcaaaactat cggagatcat ctctccattt taaataatga tattcaaatc atcgaaaata
     4261 gtggcttttc aaatttatac agtctatttt ctacattatt taccactctc ttttctatta
     4321 ttgctctttt aagttatgat attagaattg tagttttaac tatactatta acaatttgtc
     4381 ttacatacct tcctaaacca tttgctacaa aaatgcaaca ttttatgtcg ctattttcaa
     4441 ctgcaaacga agagctcata tctggattaa atgaccaatt atcaggatac aaaacacttt
     4501 attattcgaa taaaaaacct actctattaa ttcagaacac aaaaataatt aggaactata
     4561 tcacacaaaa agtaaacttt actcaaaaca gtactagaat agagacaatc atgtctgcat
     4621 tttctattat ggcacaaatg tcaattcttt tcttgacagg tttacttatc actttaggac
     4681 aagtttcaat tggttctata tcttctgtag gacaaatttc aggtaatatt tttaattcat
     4741 taacaacact taatcaacta caagtgtcaa ttcattctgt taaaccttta tttttaaaat
     4801 ttgaggtttc ttctaaacat actgagaaaa gaacagtaaa taacattgag aatattgata
     4861 tttccgattt agaatacaat tttggtaata agacagtctt ctctaaactc aatctcaact
     4921 tagttaaaaa taaaaaatac gctattatcg gtgaatctgg aagtggaaag tcaacattga
     4981 taaatattat cttaggaaat ctccagaatt atactggtca tgtaaaatac aacaatttag
     5041 agttaaaaga aattgatgaa aattccattg tttctcaagt cgcatatatt agtaattcca
     5101 cacatattta caatgacaca cttgaaaata atttaactct gtggtctcaa gacattacac
     5161 aaaatgaaat aaaaaaagct ttgaaaaatg tcaatttact ggacttacag gggcgtttaa
     5221 aggaaaaggt atctaatgat ctactttcag aaggccaaaa acaaagaatt ggtattgcaa
     5281 gagccctatt aaaaggcagt aaaattatta ttatggatga agctacagcc aatcttgata
     5341 agactaatgc tgattttatt gaaaataatc ttttaaaaaa tcctgatatt acctacatta
     5401 ccgtaacaca tcaccttagt tcagaaagag aaaaatactt tgatcaaatc attaaattag
     5461 cataataatg tgcagaagct tttggcgacc aagtcctcat cagaaatcta aaaatggaaa
     5521 aagatttaaa tacaaaataa cgaatctaag tgacatagat gtcctttaga tttgttttgt
     5581 ttatactcat ttatatcatt taagcaaaga ggtagtgtaa aataatctat gggaaataca
     5641 gagtaaatat tgctcaaatc ttttgtcaat tatataaaaa agtgttaaga aacttaaagt
     5701 aaaagcttgt tcagataata ttcacatttt ggtcagttta cccaaactaa gtatttcctc
     5761 agttacggag tactgaaagg gtaagaagag taaaccaacc gttttgtact gattctgaaa
     5821 attattttgt aacctcttat ctttacattt gttgctctct agccttgcat tgatatcaaa
     5881 aatctatata ataattaaaa acagagccag aagaaagggt gagaattatg tttatgatct
     5941 tggttctatt actaacatta gcgagtttct tatatgttac catcggtatc gccaagaaaa
     6001 tcttttcaat tttcgcctta attacaccaa ttgtagtctt aggtgttggg cttcttatcg
     6061 ccatgaatta acaaaagtca aggggggtca ctatcttaca ggtagtgacc ttttttgtta
     6121 tgcttttaat gtcaaaaaag acattaaagg agaaacatat gaaagcactt aaacttataa
     6181 gagaggacgg ttatgagaac gtccattatg ctagtttagg ggagcaaaaa tttaaaacat
     6241 tggttgttgc agataggaaa gatgaacaag aacaatggtt tgagcaacca gaaattgaat
     6301 gtgttccaga agaagtttca gatatcattc gaaactatac actcccatat attgatctcg
     6361 gaaatactga agatgagtat ttgttagaac aagcaactaa taaggttgta tcagttgtag
     6421 tagagaatgt acttgaaaca cagccattta ttccgagaaa tagaaatggt cgccctatcg
     6481 gtgaaggtgt cgcattctgc gatatgttag tagggcgtgt tcagaatgga gaacttgctg
     6541 gaacaacagt ttatattaaa caaacagaat ctggcttccg ccaaccaaaa gacttcttta
     6601 aaatggttgg gcgtattttc ccttgcaaat taactgaaat tcatatccaa actatcgaca
     6661 attataaact cacaattaat aacccatcaa actatgttgt tgaaggtagt attgagttag
     6721 ctgagtggtt aactaactac cagctttttc agcaaattga aaagagtaaa gataactctt
     6781 ctcgtaatcg agatagaaaa aacatcttgg aacgtactca tgaaggtatt gtatctgccg
     6841 tgattaacaa tggtagcaat gcaggtatca tggtaatgac caaaaaatta caaacggtat
     6901 ttattccaaa acatagggta agttatgagt gtgattcttc atttgttaac ctagacgaag
     6961 tagtatcagt ctttgatagg gtggaattta aaattactgg ggccaatacc caaaaaccaa
     7021 gtccaaatca aagagaacgt cacattatcc atgactatat tcgtatcgag ggagatatga
     7081 aagttctcga agaagcacct caagatgctt tgattgagat gattgataat aagaaagtca
     7141 taggacgatt gtttaaagtg cgatttatca gttttgatcc acagagtcta catacagtag
     7201 agttggttga ctttccaggt atcaaaatta aaatgaagga ctcaggttct ttaaatcgag
     7261 ttccttttaa gcaaggagat gaaatttcag ttgttattca agatgctcat tatcgctggg
     7321 tagacgcaaa tagcaggggg cgtcgagtgg ctaaaaaaac tgaagaagtg agcagtaatt
     7381 cacagaatac ggaagagaaa aaatgtctgt ttcgtatcaa atcacgtttc aatagtttct
     7441 atccaaatcg tgtgaacagt aaaatcttat caacattttt tactagttga gtcgcatata
     7501 gttccctctg aaatgggaac tttttgttta cgttcggaac agaaaaattt aacaaggagg
     7561 gatcatgaat catcacaata tcaatgctat tgtctattat ctgatagtct ttattcggta
     7621 tgtcttacct attaccttgg tcttcatcgg tatcggtagg tggagttatg ctaaatctat
     7681 caataatcca gagaaacaaa tcatgggagt aaattttgcc ttaaaatcaa ttttattctt
     7741 atatatcgtt acatttatcc ctattttcat tatctatgga cttgcaataa tatcaaaggg
     7801 aggaacacat agtggtgact tctctatgat tagtttcgta gaggtcttta ccctaataga
     7861 aattctagca gttggaatat tacctgcata tggtttcata caaggataca tatatcacta
     7921 tcaatggttg attacagaag gtaagaaaac aacaagctta cctcgacgaa atctattcat
     7981 tagacttgga gtaataagtc cgattgctat tgttgtattt atggaattct tgaaaagaat
     8041 gtttgcttac tagagaggtg ctttatgcgg aagatcttga aattattttt taatcgttat
     8101 tatacaaatg gtaagcgaac tatagatatc ttattgtatt gttggcgaaa atatattgat
     8161 tgggtatatc atatatcttt tggattattt gctttcaata ttattatgtt tatctttaga
     8221 tggcgtttac ctaattgggt atttatatct ttatttatat ttttgttcgt tattatggct
     8281 ttagttttat acattggtgt atggcacagt aatgttacaa gtcgtcaaat tttcgtgtta
     8341 tttgaaaaat atgatacctt gaaacgagca gaacgaaaga agattattgg taagtatgct
     8401 ataagagcag cactagcagc agtacttata tcttctggat tatatgtact aacaatcaat
     8461 aactttatct tatccctcat caatcgtatg tatgatgtag cagataatta tctcaaatat
     8521 atgccctggt caataccaaa tggaagtcag acaggtattg tattaagtta tcatacccta
     8581 aaacccttaa taggagctga ctggttcaac agtttattat tgttaactcc gattggagca
     8641 attggtctta ttttttataa tagctatgcg actgggattg ctgaaaaaac ctatgagaaa
     8701 cttgttgagc aatggctagg aggacgttac ttccaagata gtgtattaag taatattgtt
     8761 cataatcaag atgaaaaagg tcaagcaaat ttggttatgg ggcttaactc taaaactgga
     8821 gagcaagtga ttttacacgc taatgagagg acttatcaca ctattatctt tggtttgatt
     8881 ggtactggta aatcggcaac gattctgatt ccagcaatgc tacaggatat ggaaaatatg
     8941 aaatactatc tattatccta ttgtaagtat ttgatgaaag tagatgaaga actggaacaa
     9001 gaattcttta gcacagagtt tgaacgtaag aaaaaaagac gtgaattgtt ggagcagtgg
     9061 tataacaatg gttatgctgc agagcttaca aatggtattt atatgactga accatcaggt
     9121 tcgatgtatg gcacaattgt taagtacgct cataaaatgg gcttcccgga tgaaatgatt
     9181 tggactctag atcctactaa cccaggaact ggtgcaatta atattttgga tggagatact
     9241 actcaggtaa aaggggctgt tcgagactta tttaaacgat ttagtgatcc aagtgggact
     9301 ggttcttctt tcttccttaa ccaagaagga caacttactg ataatatcat tacggtcatt
     9361 aaagaggtag cgttgatacc agatgcccca ataaactaca tgttaaaagg taagtctcca
     9421 acactaactg aattctcaaa tatgttacgt tctagtgatt ggattgaggc ctatattgca
     9481 gtttttgaac aattacttaa tcgaaagaaa aggctttttg aagaaacgtt gaaaaagcat
     9541 gaagagttct tagagcagga aaaagaaaag tggttagagg aatcagagta caatcgtgaa
     9601 cacgaacaac tctttgatac tattcttaga ccagagaatg ttcatgaagc tataaacgca
     9661 tttaatgatg aaagaaataa acttgaaaat ctccaaagtt cttatgatta tttcatcaat
     9721 gctcatcaat taaattcgta cacagaggaa tattattata cctttgatgc taacgtgtct
     9781 ggtctaaaag cagttattga tgacctttca tcttcaccag aaatccgtcg tgtatttttc
     9841 tctcaaagta ccaaagattt agatattgtt ttaaaaacgg gtggaatgat tttggtcaat
     9901 tctgctaaag gtgttcttgg taaagaaaag tctcgaatgg tagcacagat ggtaacatta
     9961 gttatgcaaa atgctgctca aagacgtctt ccagataagt ctcctttctt tgctttttat
    10021 gaagatgaaa agaatggtta cttgatgcca ggtaatgata gtaacttcct agatgagtct
    10081 cgtaagttcc atgttccagt tatgcatgct tatcaacatg acgaacaaat tagggattct
    10141 attggcgaat ctggggctga tgcaatcaag atgtcttatc gtaatgcctt taccttccaa
    10201 caaacctcaa caaaggttgt agagttcatt aatctacgtt caggaggaaa acattatgct
    10261 cttaaagaaa ccaaacgtgc agcaaacgat gatctcttag caggaaatca agggaactca
    10321 agtgcgactt cagaggaagt tgtagaagaa gataacttca ctgctagaga tgcaaataac
    10381 ctagagaaat tccaagttgg tggtgttatc gtcagtgatg atgaagtctc aaatatgatt
    10441 tatttgacaa catctccgca ttttgaaatg gatatttcaa aggactcttt tgtctcacat
    10501 acacctttca ataaggtata tgggcttgga gaggaagaac cggaaatagc aaaagcatac
    10561 aagttgtgga gagcagaagt gatagaatat tacgctcaaa aagagtctga aagtcacctc
    10621 tatgaaagtg attttactga agaagaatgg caagcaatta tggatgttaa ggtaccaaat
    10681 gttgatagta atgtaaatga tacagttggt aaaaaagatg attccccatc agttaaaaga
    10741 acaatcttag tcaacgaaag tatgacagaa catagagaaa acacttcaat tagagaagag
    10801 atgacagagg acactgagca aaaagtagag gtcgatatct ctgaaattcc tccagttgtt
    10861 gaagatgaac aaactgtaaa aagcaaaaat gatatcgttg agttgaagga taaagaaatc
    10921 actcaaagag tgatcctagc tccatcatct acaccagaaa cagttgatac tcaaaatatt
    10981 caagcaccta atcaagagtt tgaattacct caagagctat ctcgtgtagc tcgtgagaaa
    11041 gcaactaaaa ctttaaaaag taatgtagaa caaatggcta ttgatgaaga agaaggtttt
    11101 tagataacat aaaaagcaac ggatttccgt tgcttttgtt ctaattttga gagtcattat
    11161 atgtttcttt attagtcttc tcagcctcct ttcttgcttt ttctgcagct tttttattct
    11221 taatttcttt ctttacctct ttaaccttat tttgtgtttc aacaactaaa tcatcaatag
    11281 ataaatctgg attttctagt tttttcaatt cgaaatagag ctgacctagt ttatcagttt
    11341 gtgatttttg ataggcctta agctgttcca tagcatcttt tttttgttgt tcgtatgcct
    11401 caatttcctt aataaaatct gattcagttt ttttgttttt tcctgccata atttgctcct
    11461 taattattaa ttatatctaa tagtatagag caagaatatt aaaagtcaac ccatgtcact
    11521 cccttgaaaa taatctattc tagggtatac tgaacactat agcgacctat tataaatgaa
    11581 tcttaattgt agttattaag ttcttaaaac cccataaaac cggttgttac gagtgttatg
    11641 taataaccag attttatgaa tgaaagtact gagagaacaa aagtctaaga gtttactgta
    11701 tttatagggc tgacgactaa tattagaaac tcgtaaatca taaatgagta aatggtggct
    11761 actatatagc tagaataaac catgtttttt agggattatc gttataaaaa taaactacga
    11821 cgttatgaat gagaggagat gaaagtatca atcagcattt tacgggtaca agagaatgct
    11881 tgtgtagggc aatagcaaag gcataacacg attttaaaag taatcgttgc actcttcgaa
    11941 aaaagaccag actatatacc ctattgattg actattttag gactacaaaa tgcaagatat
    12001 aaaaaataga gattttacct cacttatttc aaataaaaaa aataaaattc attacaccca
    12061 gaggaataaa atagctactt attttgtttc aaaaaatcaa aatatctctc tatcgttttc
    12121 agaagaggag cttatcatct tttatcatct actaaggttt ggatattttg ataaggaaat
    12181 catagacctc atttatactg cagtaactcg aaaatccaaa acccctaatt catggtttta
    12241 tcgtcttgtt ggaaaggtta atcatcctat ttcagcaagt tcggatgcca gaagcggtaa
    12301 gagaaagata tattatctga acaaaggttt tagtacctgg ttgctcacta tgattatggg
    12361 gattgataaa atacggttgc tgacgacaac gacagaatat gataggtcag tatatagcat
    12421 tgtcagtaat aatcttctag gaggaaaacc tcaaaccgta aatattcatg atctagagac
    12481 cagacgatta gttgctaaaa tcttaaacaa gctatttttt aataaccaag acaagtctgt
    12541 ttatgaccta aacatacaag tcgtatttcc tattagtcgg gttaatcttt taatggttcc
    12601 tgatgctgtt gtatttgtga acgatagccc tttttttatt gagtatgacc gagcaacaga
    12661 acgtcaaaca attctacttt ctaaaatatt tggttatgca gaagaaccag catttgaaaa
    12721 ttctagttta tttttcgttt tcgagaatag tcgtaaagat aatacgcaag tatctcaacg
    12781 tttaatgaac tttttggaat atgtaaatga ggttgaggtt gaaggtgtga ctggacatga
    12841 catgttaaag cgtaaaaata tttctttgtt cagtcaaaca acaacaaatg cttctgacgt
    12901 tattgtcgaa atcattcaag aaaagatgtt ttcaaatcat aaagattggt gtgattgcga
    12961 ggcttatgat ttttctgaac tacagaaaat agattatctt gtaaaggaga tatcagaacc
    13021 tttggatgat gataaattcc agttatcagc tgttgttgaa acagactctt gggaattgaa
    13081 agagtaccct atggtaccta tggcatatat caaaaatgac ttgattccac acttaactag
    13141 tctctatgaa gaattctcaa tagagtatga acatatgatc attgtattcc caaaagaagt
    13201 tctaccagaa aaaataaaac ttccttttga tgattatttc ttgattgttt atagatagag
    13261 taaaggtatt tgtaaatgga ttatttggaa tacttaggaa gaagacttgt atggttagtt
    13321 gtttcgcttg tagttgtaat cgtagtaaaa agaccaagag ttttgaaaag gctagccata
    13381 gttagtgatg tagctattgg aaaaatccac tactttattt ttctagcgat tgcttggttg
    13441 attcttgtca catattttat tttgtacttg tttatggtag ttactttttt cctatcatgg
    13501 cattctctat cacttaataa cgtgatggaa attattttac aaacggtatt aatcatccca
    13561 acactctata ctttatttgt accacataac gagattatca agagagttga tgaattctta
    13621 tcagcgaaaa aagaataagt cacgcgcagc aactgcaaca tataatcatt ctctcaacta
    13681 tttaaaagta tgtaagaaag tcacatgata gtttgaattt gttgtaagta tctgttggtg
    13741 ttttaaagat aaaaaagatt gaaaggtaac ggtaattaat gaaaataaaa attgaatctg
    13801 cttggaaaac cctttgtgat gcattcccca agctaaagga taatcctctt tacaagggtt
    13861 ggtatattag ctatgcgatc cttgtgacgt tatctatttt aacggtagtt gtggtgaacc
    13921 at
//
