LOCUS       NZ_JAQMJT010000004     12097 bp    DNA     linear   CON 28-FEB-2024
DEFINITION  Streptococcus salivarius strain 1001095st1_G4_1001095IJ_161003
            NODE_4_length_202274_cov_30.6445, whole genome shotgun sequence.
ACCESSION   NZ_JAQMJT010000004
VERSION     NZ_JAQMJT010000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus salivarius
  ORGANISM  Streptococcus salivarius
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 202274)
  AUTHORS   Chen-Liaw,A.
  TITLE     Human gut microbiome strain richness
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 202274)
  AUTHORS   Chen-Liaw,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2023) Precision Immunology Institute, Icahn School
            of Medicine, 1470 Madison Ave, New York, NY 10029, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.10.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 26.073790043805616x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/28/2024 01:16:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,217
            CDSs (total)                      :: 2,158
            Genes (coding)                    :: 2,099
            CDSs (with protein)               :: 2,099
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 19 of 59
            Pseudo Genes (incomplete)         :: 40 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 13 of 59
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:19:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 74011
            Orig. end                         :: 86108
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAQMJT010000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12097
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12097
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12097
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7097](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7097)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(1544..1855)
                     /locus_tag="PNU26_RS05175"
                     /old_locus_tag="PNU26_05175"
     CDS             complement(1544..1855)
                     /EC_number="3.5.1.5"
                     /GO_component="GO:0035550 - urease complex [Evidence IEA]"
                     /GO_process="GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002886559.1"
                     /locus_tag="PNU26_RS05175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_05175"
                     /product="urease subunit beta"
                     /protein_id="WP_002886559.1"
                     /transl_table=11
                     /translation="MIPGEYHVASEPIDYNGGYEAISLEVKNVGDRAAQVGSHYHFYEA
                     NEAGLQFDREKARGKRLDIPAGTAIRFEPGETKTVQLIDFGGKRRIFGFNNKVNGFLD"
     gene            complement(1867..2169)
                     /locus_tag="PNU26_RS05180"
                     /old_locus_tag="PNU26_05180"
     CDS             complement(1867..2169)
                     /EC_number="3.5.1.5"
                     /GO_function="GO:0009039 - urease activity [Evidence IEA]"
                     /GO_function="GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /GO_process="GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889924.1"
                     /locus_tag="PNU26_RS05180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_05180"
                     /product="urease subunit gamma"
                     /protein_id="WP_002886558.1"
                     /transl_table=11
                     /translation="MQLTMREQEKMMISLAAMIAQRRKDKGIKLNHPEAVALITDYVLE
                     GAREGKTVAQLMDEARNLLTREDVMEGIAEMIPMIQVEATFTDSTKLVTVHDPIQ"
     gene            complement(2194..2709)
                     /locus_tag="PNU26_RS05185"
                     /old_locus_tag="PNU26_05185"
     CDS             complement(2194..2709)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889220.1"
                     /locus_tag="PNU26_RS05185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_05185"
                     /product="AmiS/UreI family transporter"
                     /protein_id="WP_002886548.1"
                     /transl_table=11
                     /translation="MLGVILLYVGMVLMSNGLHRLEGIPDKSNVVMNIFTGGLGLILNI
                     IVIAYGACTGQGAEWFYGSATGLLFAFTYLYSAINTIFDFDQRLYGWFSLFVAINTLPA
                     GILCLTSGYGGNAWYGIIWFLWGILWLTAFIEINLKKNLGKFVPYLAIFEGIVTAWIPG
                     LLMLWGKW"
     misc_feature    2768..2782
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 25.38"
                     /tool="antismash"
     misc_feature    2871..2889
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.75"
                     /tool="antismash"
     gene            3113..3799
                     /locus_tag="PNU26_RS05190"
                     /old_locus_tag="PNU26_05190"
     CDS             3113..3799
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PNU26_RS05190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="PNU26_05190"
                     /product="urease cluster protein"
                     /protein_id="WP_230310563.1"
                     /transl_table=11
                     /translation="MMETNNYTTNLQLVSQEYSDEKTSLQTKTKKLLEYFSPKDIWKIK
                     GLIVACLVACFLYVTYLTHFSFAFPTTLVLISTIELGCRLLGGKAKRMRLESAIFMVTS
                     LLQGLQISLWGLHDNWGSAQILCLHASILLYLMARTGQSSQDRLGIMVWLDLIYTSCIF
                     PLKYFFIGAWTLLYKTAPKQELSEEEKHAVQNHKWMILISIMIALVLTVFAASQLSQVL
                     PAFGQL"
     gene            3866..4507
                     /locus_tag="PNU26_RS05195"
                     /old_locus_tag="PNU26_05195"
     CDS             3866..4507
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /locus_tag="PNU26_RS05195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_05195"
                     /product="DUF4153 domain-containing protein"
                     /protein_id="WP_230310562.1"
                     /transl_table=11
                     /translation="MFSRLITSIPLALGLFAQLVATYLAKEKPLSQKRFEGRLKTTRQF
                     PKVAAYIVLGSLCLTYALFILTATSQMGELLSLNQVNIPAHQASHIAVQGFWQLVRVST
                     LNLGILAGLYIFSKENFFTHKGLRLLSTLLFTFASLFALIAGWKLFGVYIALYGVTPRR
                     LLSGWLVTVLFIWCVLTLIRLYKPIQATRLAVFYATISFTLVSLLGSFIL"
     gene            complement(4558..4890)
                     /locus_tag="PNU26_RS05200"
                     /old_locus_tag="PNU26_05200"
     CDS             complement(4558..4890)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003097990.1"
                     /locus_tag="PNU26_RS05200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_05200"
                     /product="DUF805 domain-containing protein"
                     /protein_id="WP_002886546.1"
                     /transl_table=11
                     /translation="MLEAYKNFWLGYLDFYGRTKRSDYWWVVLINGIIGSLIFLLMDLL
                     GETRAAGFTLTAFILFIAATALPTISMQIRRLRDANFNVAWIVLKATPLLVVLWVMYAF
                     PTRDSK"
     gene            complement(5001..7097)
                     /locus_tag="PNU26_RS05205"
                     /old_locus_tag="PNU26_05205"
     CDS             complement(5001..7097)
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535846.1"
                     /locus_tag="PNU26_RS05205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_05205"
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_175057392.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 2.8e-44, bitscore:
                     143.8, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 2.6e-260, bitscore:
                     859.3, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKYPYISQVDQRDCGVAALAMILKHYGSSYSLAYLRELAQTSRE
                     GTSALGLVEAAKQLGLETQAIRADLDLFKQENLSYPFIVHVVKEGGLQHYYTVFGQIKG
                     QLVIGDPDPSKKVIKMPLEEFAKEWTGVTLFFVPGETYTKYKEDVPGLLSFLPILFRRK
                     GLIAVIVLLSFLVTLVNIIGSYYLQAIIDRLIPQGAYNLLTMISLGLCISYLAQQVFTF
                     FKDYLLHRLGNYLSIAVILPYIKHVLSLPISFFSSRRTGEITSRFGDANTIIDALASTI
                     LSIFLDVTIVMTLAVALIVQNQSLFVMTLTVVPLYVLIIVVFYKLFEKENYQLMEANSQ
                     VNTAVIDDLRGIETLKSLRVEDRRYREIEVKFHDYLKKSLSKAKWQLTQDGLKTGVQLV
                     SNVFILWYGAQLVMERQLSAGQLITYNMLLNYFTTPLINIINLQSKIQQAKVANNRLQE
                     VYVVDKEEKGKLKELSFKQLAFKGVSHRFSYQQETLSKIDLTIHKGEKIALMGQSGSGK
                     TTLAKMLSGYYTKSSGHVTLDKDTISHAELRQMVTYVPQQTYVFTGTILENLLLGFEGE
                     VDEKKLIKVCQQADILEDIQRMPLGFQTQVSEDGGLSGGQKQRLAIARALLSKQPILIF
                     DEATSGLDSDTESRVMANLAKIKRTMIFIAHRNSVRQHVSRVVTMVSGQIESDSANFNP
                     FQFI"
     misc_feature    complement(7248..7263)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.94"
                     /tool="antismash"
     gene            complement(7272..8171)
                     /locus_tag="PNU26_RS05210"
                     /old_locus_tag="PNU26_05210"
     CDS             complement(7272..8171)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883753.1"
                     /locus_tag="PNU26_RS05210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_05210"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_073685698.1"
                     /transl_table=11
                     /translation="MNIKDSIGLRIKTERDRQQMSREVLCLDGAELTVRQLIRIEKGES
                     LPSLDKLSYIAKRLGKNMTDLLDHDNITIPDEYYEMKNRLIKFPTYRNPERIKAKLALI
                     EEVYEKFFELLPEEELLTLDILENILSFTSWEESPKVEEIYEDLFEQVKRKKKFSTNDL
                     LVIDYYFFHLYGRKQYDKKLFERIVKRVLNQEIWTDDVYNIVLFNDLMAIAALKIFHNS
                     FSDFLTVVDKALAVIEKSQFYSYKPNVFVLKAKYELLHKGNKKEAAENYDKAIMFASVL
                     EDSVLEESIKAGKAADGL"
     gene            complement(8350..9000)
                     /locus_tag="PNU26_RS05215"
                     /old_locus_tag="PNU26_05215"
     CDS             complement(8350..9000)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535842.1"
                     /locus_tag="PNU26_RS05215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_05215"
                     /product="phosphatase PAP2 family protein"
                     /protein_id="WP_022496033.1"
                     /transl_table=11
                     /translation="MKHKHTHYIYASFALLLFVALGYMVKFYPEALTGFDSNIQTWVRG
                     DLPAGLTRFFKTITILGNTPVQAIIAIVAVIWLYLRQYKAEAIFVGASGLLASILIVSL
                     KYIYQRQRPSITHLVHASGYSFPSGHSLGTFMILGAIAIVLAQRLAKKESKIAVYGITG
                     LLIFLVGLSRIYVGVHYPTDVLAGFTLAFGLLNAIYPTYDRIRFEWRFQSKQK"
     gene            complement(9003..9560)
                     /locus_tag="PNU26_RS05220"
                     /old_locus_tag="PNU26_05220"
     CDS             complement(9003..9560)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946140.1"
                     /locus_tag="PNU26_RS05220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_05220"
                     /product="ECF transporter S component"
                     /protein_id="WP_022496034.1"
                     /transl_table=11
                     /translation="MTNTRKLAYIAILSAVSFLLLYFSFPLIPAADFLKVDFSILPVLI
                     ALVIFDFKSAIGVLLLRSLLKLLLNNGGPGSMIGLPMNFVALGVFVWGLSYFWKKNQTS
                     KNYILGSVLGTVLLTVAMVALNYIYAVPLYAKFANFDIAQFIGLYKYLFAMVVPFNLLE
                     GLIFAVAFALIYAPLKSILVKL"
     regulatory      complement(9641..9763)
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     gene            complement(9850..10359)
                     /locus_tag="PNU26_RS05225"
                     /old_locus_tag="PNU26_05225"
     CDS             complement(9850..10359)
                     /EC_number="2.1.1.207"
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /GO_function="GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949522.1"
                     /locus_tag="PNU26_RS05225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_05225"
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="WP_002883735.1"
                     /transl_table=11
                     /translation="MSRNHIVLFEPRIPQNTGNIARTCAATNSPLHIIRPMGFPIDDKK
                     MKRAGLDYWDKLDITYYDNLADFMSKNQDGRLHLVTKFADKTYSDENYADGGDHYFMFG
                     REDTGLPEDFMREHPEKAIRIPMNDEHVRSLNVSNTVCMIVYEALRQQQFAGLELSHRY
                     QNDKLK"
     misc_feature    10518..10531
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.01"
                     /tool="antismash"
     gene            10651..12000
                     /gene="trkA"
                     /locus_tag="PNU26_RS05230"
                     /old_locus_tag="PNU26_05230"
     CDS             10651..12000
                     /codon_start=1
                     /gene="trkA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262566.1"
                     /locus_tag="PNU26_RS05230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PNU26_05230"
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="WP_022496357.1"
                     /transl_table=11
                     /translation="MRIIVVGAGKVGTALCRSLVEEKHDVILIEEKEEVLKRLSKRYDV
                     MGFAGNGANFKILEQAEVNNCDVFIAMTDKDEVNMISAVLAKQMGAKETIVRVRNPEYS
                     NAYFKDKNFLGFSLVVNPELLTARYIANTVEFPNALSVETFANGRVILMAFKVTENSQL
                     SGMTMEQFRKKFGNILVCTIDRQGELIIPDGNATMQIGDKIYVTGKRVDMALFHSYIKP
                     KSIKKLLLIGAGRISYYLLNILKNNRIKVKLIEQKMDRAQTFSQVFPEVSVILGDGTAK
                     DLLLEESASSYDAVATLTGVDEENIITSMFLESLGIHKNITKVNRTSLLEIIDTRETTT
                     IVTPKSIAVDTVMHFVRGRYNAKDSSLDALHHVANGRIETLQFQIKENNKIAGKKLSEL
                     EFANGVLIAAIIRNGKSIFPTGEDRLAIGDKIVIVTLLQNVTHIYDLLKR"
ORIGIN
        1 caatttcttc tttgataccg tgatcataag catattgaga gacaaacgtt agatttgttt
       61 catgaaccgc ttgaccgtaa gcaccatata atttacgcat gatacgtggt tgaggtgttg
      121 gaagacttga accagcgtca cccataacac cataagttaa cataccaccc ttaagcacca
      181 acttaggttt tgcaccaaat tgtgctggtt cccagataac caaatctgca aatttaccaa
      241 cttctacaga accgatatag tctgcaatac cgttagtgat agcagggtta attgtatatt
      301 tagccacgta acgtttgata cggttattat cgttaaactc tgaatcccct tcaagtggac
      361 cacgctgagc cttcatctta tcagctagtt gccaacaacg cattgctact tcaccgacac
      421 gtcccattgc catggcatct gaagtcatga tactaagggc acccatatcg tgaagaacgt
      481 cttctgcagc cacagtttgt ttacgtacac gtgattcagc aaaagagacg tcttctggga
      541 tttttggatc caagtggtga caaaccataa ccatatctaa caactcacca atagcatttg
      601 tggtgtatgg gttagttggg ttagttgatg atggcaaaat attttcctta ccagcaaaga
      661 ccatgatatc tggagcatgt ccaccacctg aaccttcggt atggaaggta tgaacggtac
      721 gaccttggaa ggattccaaa gtattttcca taaatccacc ctcattcaaa gagtcagtgt
      781 ggaccgcaaa ggaaacatcg tatttatccg caactttaag ggaattatcg attcctgcgt
      841 aagtcgcacc ccagtcctcg tgtgtcttaa ttcctgctgc accagcttta atttgctctt
      901 caaccacttc agtctcagaa ccaacacctt tggcaaggaa accaaagtta gcaggcatat
      961 catcaacagc ttgcaacata cgagtaatat ggaatttacc tggtgtacat gttgttgcat
     1021 tactaccgtc agcgggacca gtaccaccac caaagagggt cgtaattcca ttatccaaac
     1081 caaattcagg aaggtcagca gaaatataat gtacgtggag gtcaatacca ccagcagtca
     1141 caatcaaacc ttctgcagca atggcttctg tactagcacc cacaacaaag tccacattgt
     1201 ccattgtatc tgggttacca ccgcgaccaa tagcaacaat cttaccatca cgaataccga
     1261 tatctgcttt aataatacct gtatagtcaa tgatggttgc acctgtaata atggtatcaa
     1321 caactgatgg attgtcacgt gtttccgtag cactaacacc cataccatca cgcaaaactt
     1381 taccgccacc gaacttagat tcctgtccat aaacagtaaa gtctttttca atggctgcaa
     1441 aaagattggt atctccaaga cgtacgctat cacctacagt tggtccatag tgttgagcat
     1501 actcttcacg atccatttta aaactcatcg atttgtcctc tttctagtct aagaaaccat
     1561 tgaccttgtt attgaaacca aaaatacgac gtttccctcc aaagtcaata agttgtactg
     1621 ttttcgtttc acctggctca aaacgaatgg ctgtacctgc tggaatatct agacgtttgc
     1681 ctcgcgcctt ttcacgatca aattgaagac cagcttcgtt tgcttcataa aaatggtagt
     1741 gagaacccac ttgagcagca cggtcaccca catttttcac ttcaagacta atggcttcgt
     1801 aaccaccgtt ataatcaatt ggctcactcg ccacatggta ttcacctgga atcattacac
     1861 ttctccttac tgaataggat catgaacagt aaccagtttt gtactgtccg taaaagtagc
     1921 ttcaacttga atcattggaa tcatttctgc aataccttcc ataacatctt cacgtgttaa
     1981 gagattgcga gcttcatcca tcagttgggc aacagtttta ccttctcttg caccttcaag
     2041 cacatagtca gtgattaaag caactgcttc tggatgattc aatttgattc ctttgtcttt
     2101 acgtcgttga gcaatcatag ccgcaaggct aatcatcatt ttttcctgct cgcgcattgt
     2161 caattgcatc gtttttcctc cttaaatcaa tgcttaccac ttgccccaaa gcatcaaaag
     2221 ccctggaatc caagctgtta caattccttc aaaaatagct aggtaaggga caaattttcc
     2281 tagattcttc ttaagattaa tttcaataaa ggcagttagc cataggatac cccacaagaa
     2341 ccaaataata ccataccaag cattaccacc gtatccagat gttaagcaaa gaatccctgc
     2401 tggtagcgta ttaattgcca caaataaact aaaccaccca tacaaacgtt gatcaaaatc
     2461 gaaaatcgta ttgattgctg agtaaagata ggtgaaagca aataaaagac cagtagcgct
     2521 accataaaac cattcagcgc cttgccctgt acaagctccg taagcaatga caataatgtt
     2581 gagaatcaaa cccagaccac ccgtaaagat attcatgaca acatttgatt tatcaggaat
     2641 accctctaaa cgatggagtc cattactcat caaaaccata ccaacataga gtaggatgac
     2701 acctaacata agaacctcct aagtttttta tgttaatatc atactacttt gacaacacat
     2761 gtcaacgaat tttcagaaat tttgcaacat ttagatggca gtcaatctaa catatagtgt
     2821 caaaaaatga catcaccttt ggtgatcctc gatagaagtc ctttgctcaa tcctagataa
     2881 acaaacagtt aatctctaaa aaactatctt tagcatttga cagtttctga caatatagcc
     2941 cgtacaatct atcattcaat gtgaacataa tattttttac tcacctattt ttccaactca
     3001 ttctccagac ctaagtccat agacctattt ttagtaattt tacagttggg tgacaaaaat
     3061 gaacattttt tatgataaag tcttttttgg actaacatcc aaaaggagaa ttatgatgga
     3121 aacaaataac tatactacta atttacagct agtctcccag gaatattccg acgaaaagac
     3181 tagtcttcaa accaaaacca agaaactctt ggaatatttt tcaccaaaag acatttggaa
     3241 aattaaggga ctgattgtcg cctgtctcgt ggcctgcttc ctgtatgtca cttacttgac
     3301 ccatttttcc tttgcctttc caacaaccct tgtcttgata agtacgattg aactgggctg
     3361 tcgtctatta ggtggaaaag caaagagaat gcgacttgaa tcggcaatat ttatggtcac
     3421 tagtctacta caaggtttac aaattagtct ttggggtctg cacgacaact gggggagcgc
     3481 tcagattctt tgccttcatg cgagtatcct cctttactta atggcacgta ctggacaaag
     3541 tagtcaagac cgtttgggaa ttatggtttg gctagacctc atatacacta gctgcatctt
     3601 cccactgaaa tatttcttta tcggagcctg gacactactc tataaaacag cacctaaaca
     3661 agagctttct gaagaagaaa agcacgcagt tcagaaccac aaatggatga ttctcattag
     3721 tatcatgatt gccttggtac tgactgtatt cgctgctagt cagcttagtc aggtcttgcc
     3781 tgctttcgga caactttgac aacagttctt ccagacactt gctactctac ttgatttcac
     3841 tatcaccttc aaggataacg gattattgtt ctctcgttta ataaccagta tccctctggc
     3901 tctaggactt tttgctcaac tcgttgcaac ctatttggca aaagaaaagc cacttagtca
     3961 aaaacgattt gaaggccgtc ttaaaacaac ccgtcagttt ccaaaagtag cagcttatat
     4021 cgtactcgga agcctctgcc ttacttatgc cctattcata ttaactgcca ctagtcaaat
     4081 gggagaactc ctttctctca accaagtcaa cattccagct catcaagcct cacatattgc
     4141 tgttcagggc ttctggcaat tagttcgagt ctctaccctt aacttgggaa tcctagctgg
     4201 actttacatt ttttctaaag aaaacttctt cacccacaag gggcttcgtt tgttgagtac
     4261 cctactcttt acttttgcta gcctctttgc ccttatcgcc ggctggaaac tctttggcgt
     4321 ttatatagca ctctatggtg taacgccacg tcgcttactg tcaggttggc ttgtcacagt
     4381 actctttatt tggtgtgtcc taacacttat tagactttat aagcctatcc aggcaacccg
     4441 tctcgctgta ttttatgcaa ctatcagctt caccttggtt agcctcttag gtagctttat
     4501 tttataagaa aaaagagtgg aactagatta actagtttca ccctttttat attggagtta
     4561 ttttgaatct cttgtaggaa aggcatacat cacccaaagt actactagta gtggtgtcgc
     4621 ttttaataca atccaagcaa cattgaaatt ggcatcacgt agacgtcgaa tctgcataga
     4681 aatggtagga agcgccgttg ctgctataaa tagaatgaaa gctgtcaatg taaagccagc
     4741 agctcttgtt tccccaagta agtccatcag taggaaaatc aaactaccga taattccatt
     4801 aatcaagaca acccaccaat agtctgatcg ttttgtacga ccataaaaat cgagatagcc
     4861 taaccaaaaa tttttatatg cctctaacat gtttacacct cttaatagtc tgttaccagt
     4921 atactatatt aataagatag gtgcaagatg tgaaaattta gacttcttaa gttatctaca
     4981 tacataatag gaagattatt ttaaataaac tggaatggat tgaaattagc actgtctgac
     5041 tcaatctggc cactcaccat ggtcactaca cgactgacat gttgacgtac actattgcgg
     5101 tgagcaataa aaatcattgt tcgtttaatc ttagcaagat tagccatgac cctgctttca
     5161 gtatcactat cgagaccact ggtagcttcg tcaaaaatca agataggttg tttggacaat
     5221 agagcccttg ctatagctaa tctttgtttt tgaccgccag acaaaccacc gtcttcagat
     5281 acttgggtct gaaagcctag tggcattctt tgaatatcct ctaagatatc agcttgttgg
     5341 caaactttaa taagcttttt ctcgtcaact tctccttcaa atcctaatag aagattctct
     5401 aaaatcgtcc ccgtaaacac ataggtctgc tggggaacgt aagtcaccat ttgacgcaac
     5461 tccgcatggc taatggtatc tttatctagg gttacatgac cactagattt cgtataataa
     5521 cctgacaaca ttttagctaa ggtcgttttt ccagaaccac tctgacccat gagggcgatt
     5581 ttctcaccct tatgaatcgt taaatcaatc ttactaagag tctcttgctg ataactaaaa
     5641 cggtgactaa ctcctttaaa agcaagttgt ttaaaagata actctttaag ttttcctttt
     5701 tcttccttgt ccacaacata tacttcttga agacgattat tggcgacttt ggcttgctga
     5761 attttagatt ggagattgat gatattaatt aaaggagttg taaaataatt caacaacata
     5821 ttataggtaa tcaactgacc tgcagatagt tgcctttcca taaccaattg tgccccatac
     5881 cagaggataa agacattaga aactaactga acccctgtct ttaacccatc ctgcgttaat
     5941 tgccacttgg ccttagacaa ggacttcttc aaatagtcat ggaatttcac ctcaatttcc
     6001 cgataacgtc tatcctcaac tctcaaggat tttaaggttt caatacctcg caaatcatca
     6061 ataacagcgg tattgacctg actatttgcc tccatcaatt gataattttc cttttcaaaa
     6121 agcttataga ataccacaat gattaaaaca taaagaggaa ctaccgtgag agtcatcaca
     6181 aaaagtgact gattttgtac aataagagcc acagctagag tcatcacaat ggtcacatct
     6241 aggaaaatcg ataaaatcgt tgaagctaga gcatcgataa tagtgttagc atctccaaaa
     6301 cgtgacgtga tttcaccagt acgacgtgaa ctaaagaaac taataggcaa ggaaaggaca
     6361 tgcttgatat aaggaagaat gacagcaatg gacaggtaat tacccaatcg atgtagaaga
     6421 taatctttga aaaaagtaaa aacttgttga gcaagataag agatgcataa gcctagcgaa
     6481 atcatggtca gaagattata ggccccctga ggaatcaaac ggtcgataat agcttgcaaa
     6541 taataagaac caataatatt aacaagggtc actagaaaac ttaataagac aataactgca
     6601 atcaaaccct tacgacgaaa caaaattggt aaaaatgaaa gaagtccagg tacatcttct
     6661 ttgtatttag tgtaggtttc tcctggaaca aaaaagagag taactcccgt ccactctttg
     6721 gcaaattcct ctaagggcat tttaatgacc ttcttgctag gatctggatc accaataaca
     6781 agctgcccct tgatttgacc aaacaccgta taataatgtt gcagcccacc ttctttaacc
     6841 acatgtacaa taaaaggata agataggttt tcttgtttaa acaagtctaa atccgcacga
     6901 atagcctgag tttcaagacc caactgcttg gccgcttcca ccaaaccaag cgcagaagta
     6961 ccttcacgag aagtctgagc caactctcgc aaataagcta aactataaga tgaaccatag
     7021 tgtttaagaa tcatagccaa ggcagcgaca ccacaatcac gctgatcaac ctgtgaaata
     7081 tatggatatt ttgacatggt gactccttat taaagacaac ctgcaaaata aggcaagata
     7141 gtaataagta gtgagaatag tgtaaataat tttagttttt tcaaaataaa actcctttta
     7201 actgttattt atgtttctat agtaaacatt taaccattaa aaaagtagtg acatttatgt
     7261 caccaccatt tctataaacc atctgccgct ttacctgcct ttatactttc ttctagcacc
     7321 gagtcttcta aaacggaagc aaacattatg gccttatcgt aattttctgc agcctctttc
     7381 ttgtttcctt tatgcagaag ttcatatttg gctttgagaa caaaaacatt aggtttatag
     7441 ctatagaatt gtgatttttc tataacagct aaggctttat ccacaactgt taagaagtct
     7501 gagaaggaat tgtgaaaaat cttcaaagca gcgatagcca tcaaatcgtt aaataaaaca
     7561 atattataga catcatctgt ccaaatttcc tgatttaata ctctctttac gattctttca
     7621 aaaagttttt tatcatactg ctttcttcca taaagatgga agaaataata gtcaataact
     7681 aataaatcgt tagttgagaa tttcttcttc cttttgactt gttcaaacaa gtcttcatat
     7741 atttcctcaa ctttaggact ctcttcccaa ctagtaaaac tcaaaatatt ttccaaaata
     7801 tctagagtta acaattcttc ttctgggaga agttcaaaga atttctcata gacctcctca
     7861 attaaggcaa gtttagcctt tattctttct ggatttctat acgttggaaa cttaatcaga
     7921 cgattcttca tctcgtagta ttcgtcaggg atagtaatat tatcatgatc caataaatcg
     7981 gtcatatttt ttcctagacg ttttgcaata taagataatt tatccaaaga cgggagcgac
     8041 tccccttttt caatgcgaat taactgacga acagtcaatt ccgcaccatc taaacataac
     8101 acttcacgtg acatctgctg gcgatcacgc tcagttttga ttcttagtcc aatgctgtct
     8161 tttatgttca agagacactc ctttatttcg aaattttaaa aacattatat cataattata
     8221 atataattaa tatacagtta atgcattttt aacagaacaa tagtcaaaat aagctatttt
     8281 tatacatttt atataggaaa gacctttctt tgtatcaaaa aaacatgcag tttaaaacta
     8341 catgtttttt tatttctgtt tactttggaa tcgccattca aagcgaatgc gatcataagt
     8401 tggataaata gcattaagca acccaaaggc taaggtaaag ccagctaaaa catctgttgg
     8461 ataatgaaca cctacataaa tacgcgaaag tccaacaagg aaaatcagaa gcccagtaat
     8521 accataaact gctattttag attctttttt cgccaaacgc tgagcaagaa caatggcaat
     8581 tgctccaaga atcataaaag ttcccaaaga gtgaccacta gggaaagaat aaccactagc
     8641 gtgcactaaa tgcgtaattg atgggcgttg acgctggtaa atatacttca aacttacaat
     8701 caagatacta gcgagaagcc cactggcacc tacaaagata gcttctgctt tatactgacg
     8761 tagataaagc cagattactg ctacgattgc aataatagcc tgtacgggcg tattcccaag
     8821 tatggtaatc gttttaaaaa agcgtgtcaa tcctgctggt aagtctcctc gaacccaagt
     8881 ttgaatgttg ctatcaaatc ctgtcaaggc ttcagggtag aacttaacca tgtaaccgag
     8941 agcaacaaat aacaataagg caaaagaagc ataaatataa tgtgtgtgtt tgtgtttcat
     9001 atttataatt ttactagaat agacttaagt ggagcataaa tcagagcaaa ggctactgca
     9061 aaaattaatc cttcaagtaa attgaaaggt actaccatag cgaaaaggta tttatagaga
     9121 cctataaatt gcgcaatatc aaagttggca aatttggcat agagtggtac tgcataaata
     9181 tagttaagtg ctaccatagc aactgtaagc aagacagtac ccaaaacaga tccaagaata
     9241 taattcttac tcgtttgatt ctttttccaa aaatagctta ggccccaaac aaagactcct
     9301 agagcaacga aattcatagg aagtccaatc attgatcctg gaccaccatt gtttagcaag
     9361 agcttcaaca atgaacgaag taagagaacg ccaatagcac tcttaaaatc aaagataacc
     9421 aaggcgataa gaactggaag gattgaaaaa tcaaccttca aaaaatctgc tgctggaatc
     9481 aatggaaatg aaaaataaag caataagaat gaaactgcgg ataaaatggc aatataagcc
     9541 aatttacgtg tgtttgtcat aaaaaaattc ctccaaattt taacaatttg aaagaagttc
     9601 ccaatgtatt tgcctatgtc aaaaaagact acgcaaaaag actggtcttc ttccatccag
     9661 actgtactgt cggttgagga atcgcacctc atcagcttgc gctcgcggac ttatccatag
     9721 aatcaccgcc ggtcgggaat ttcaccctgc cctgaagacc ctttaagatt aacacaaaaa
     9781 agccttgatg acaaggcttt cgatttatag atttctaatc aaatgatagt caacgactat
     9841 taatagtctc tattttaatt tatcattctg gtaacgatgg ctcaactcta accccgcaaa
     9901 ctgttgctga cgaagtgctt cataaacaat catacaaaca gtattggaca catttagact
     9961 acgcacatgt tcatcattca tcggaatacg aatagctttt tcaggatgct cacgcataaa
    10021 atcttcaggc agacccgtat cttcacgacc aaacataaaa tagtggtctc cgccatcagc
    10081 ataattctcg tctgaataag tcttatctgc aaacttcgtg actaaatgca aacggccatc
    10141 ttgattttta gacataaaat cagccaaatt gtcatagtag gtgatgtcaa gtttatccca
    10201 gtagtcaagg cctgcacgct tcatcttctt gtcatcaatt ggaaatccca taggacgaat
    10261 gatatgaaga gggctgttag tcgctgcaca ggtacgagcg atattcccag tattttgcgg
    10321 aatgcgaggt tcaaacaaga caatatgatt acgtgacatc tcaattctcc tagacataaa
    10381 aaataccatg tcgcccggag tcaagctcag cagacgacat ggttgtggac gttcattagc
    10441 ttaaatcatg acaggttaga tttaaaataa cgaatctttg aatatatttt agcacttcta
    10501 gtgtaaaagt caacactatt ttaagaaaaa aaagtaactt ttataacttc attattcact
    10561 ttaaataaga acagttgata aagctagccg aaaagggtga tttttcagct aaaatagagt
    10621 atcatagtaa tgattatttg gaggtaactc atgcgtatta ttgtcgtagg tgccggtaaa
    10681 gtcggtaccg cactctgtcg ctctcttgtt gaagagaagc acgatgttat tttaattgaa
    10741 gaaaaagaag aggtcctcaa acgactcagc aaacgttacg atgtgatggg ctttgctggt
    10801 aatggggcta atttcaaaat cttggaacaa gccgaggtta acaactgcga tgtttttatc
    10861 gctatgactg ataaagatga agtaaacatg atttcagccg ttctggctaa acaaatgggt
    10921 gccaaagaaa ccattgttcg tgtgcgtaac ccagaatact caaatgctta tttcaaagac
    10981 aagaactttc ttggattctc attagtagtt aatcctgaac ttttaacagc tcgctatatt
    11041 gccaatacag ttgaatttcc aaatgctcta tcagtagaaa cctttgctaa cggacgtgtt
    11101 atcctgatgg catttaaagt cactgaaaat agccaattgt ctggtatgac aatggaacaa
    11161 ttccgtaaga aattcggcaa tattctagtt tgtacgattg accgtcaagg tgaactcatc
    11221 attccagatg ggaatgctac catgcaaatt ggggataaga tttatgtcac tggtaaacgt
    11281 gttgatatgg cgcttttcca cagttacatc aagccaaaaa gcatcaaaaa acttttgttg
    11341 attggtgctg gacgtatctc ctactacttg ctcaatatct tgaaaaacaa tcgtattaag
    11401 gtcaaattga ttgagcaaaa aatggaccgt gcacaaacct ttagtcaggt cttccctgaa
    11461 gtttctgtta tcttaggtga cggtactgct aaagaccttc ttctcgagga aagtgcatcc
    11521 agttatgatg ccgtagctac cctaactgga gtagatgaag aaaatatcat cacctctatg
    11581 ttcctcgaaa gccttggtat tcacaagaat attactaaag tcaaccgtac cagtctttta
    11641 gagattatcg atacacgtga gacaacaaca attgtcacac ctaagagtat tgccgttgac
    11701 actgtaatgc actttgtccg tgggcgttat aatgctaaag attctagcct cgatgccctt
    11761 caccatgtgg ctaatggtcg tatcgaaacc ttgcaattcc aaattaagga aaacaacaaa
    11821 atcgctggga agaaactttc tgagcttgaa tttgctaatg gtgtccttat cgctgctatt
    11881 attcgcaatg gaaaatctat tttcccaacg ggagaagaca gactcgctat tggggataaa
    11941 atcgtgatcg ttacactctt acaaaatgta actcatattt acgacttgtt gaagaggtaa
    12001 gctgccatga atcgatctat tgtccgtttt cttattgcca agcttctgct tattgaggct
    12061 gctctcttgg ttgtaccact tatcgttgcc ctcattt
//
