LOCUS       NZ_CP116257            10642 bp    DNA     linear   CON 15-DEC-2024
DEFINITION  Streptomyces lienomycini strain DSM 41475 chromosome, complete
            genome.
ACCESSION   NZ_CP116257
VERSION     NZ_CP116257.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces lienomycini
  ORGANISM  Streptomyces lienomycini
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7863969)
  AUTHORS   Ding,W.
  TITLE     Streptomyces riduolalong sp. nov., isolated from the soil of Tibet
            exhibits against Gram-positive bacteria activity
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7863969)
  AUTHORS   Ding,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) School of life sciences and biotechnology,
            Shanghai Jiaotong University, 800 Dongchuan, Shanghai, Shanghai
            200240, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: GS De Novo Assembler v. August-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_027947595.1-RS_2024_12_13
            Annotation Date                   :: 12/13/2024 16:03:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,183
            CDSs (total)                      :: 7,093
            Genes (coding)                    :: 6,850
            CDSs (with protein)               :: 6,850
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 243
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 243
            Pseudo Genes (frameshifted)       :: 141 of 243
            Pseudo Genes (incomplete)         :: 121 of 243
            Pseudo Genes (internal stop)      :: 14 of 243
            Pseudo Genes (multiple problems)  :: 26 of 243
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:34:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6635106
            Orig. end                         :: 6645748
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116257.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10642
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="27"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10642
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10642
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5642](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5642)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    complement(1497..1513)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.58"
                     /tool="antismash"
     misc_feature    complement(1502..1516)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.96"
                     /tool="antismash"
     misc_feature    1525..1538
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.26"
                     /tool="antismash"
     misc_feature    1525..1540
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.93"
                     /tool="antismash"
     misc_feature    complement(1527..1541)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.51"
                     /tool="antismash"
     misc_feature    complement(1528..1543)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.68"
                     /tool="antismash"
     tRNA            1817..1892
                     /anticodon=(pos:6636956..6636958,aa:Arg,seq:tct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="BJ961_RS30270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /product="tRNA-Arg"
     gene            1817..1892
                     /locus_tag="BJ961_RS30270"
     gene            1923..2948
                     /locus_tag="BJ961_RS30275"
     CDS             1923..2948
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028521.1"
                     /locus_tag="BJ961_RS30275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="WP_271415958.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 9.8e-51, bitscore:
                     170.3, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLRCAVLDDFQRVATEIADWSPVAGEVDVVSFDTHFADEDTLAA
                     ALADVDIVVTLRERVSFPGSLIARLPRLRLIVASGMRNGVIDYAAAEAHGVTVCGTASS
                     STPPVELTWALLLGLARGIVQEGNGLRGSGPWQQTVGADLHGRTLGLLGLGRIGGRVAR
                     VGLAFGMRVSAWSQNLTRERAEEVGVERAPSKEELLRTADFVSVHLALGERTRGLLGPA
                     ELALLKPTAYLVNTSRAAIVDQGALLAALHEGRIAGAGVDVFDIEPLPAGHPMRSAPRL
                     LATPHLGYVSRANYATYYGQAVEAIAAYLAGSPVRRLPVPAAPSQGPVPGPAGSPAPTM
                     S"
     gene            complement(2955..3532)
                     /locus_tag="BJ961_RS30280"
                     /pseudo=""
     CDS             complement(2955..3532)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976102.1"
                     /locus_tag="BJ961_RS30280"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="DUF2087 domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MPSALAALPALFAEESRVRAFAAVALGAGDAAGWPWRPASPRRRR
                     PVRCAGCGSRAWCRPGTTADSSSHTTVSGSWPAPAGRCAGRPRNPPGGAGTARPT"
     misc_feature    3668..3679
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.07"
                     /tool="antismash"
     gene            3813..4088
                     /gene="chpF"
                     /locus_tag="BJ961_RS30285"
     CDS             3813..4088
                     /codon_start=1
                     /gene="chpF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028524.1"
                     /locus_tag="BJ961_RS30285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="chaplin ChpF"
                     /protein_id="WP_271415959.1"
                     /transl_table=11
                     /translation="MSRIAKAAGVALGAGAVALSGAGLAMADAGAQGAAVGSPGVLSGN
                     VVQVPVNVPVNVCGNTIDVIGLLNPAFGNACENGDDHDDKSGGYGG"
     gene            complement(4151..5017)
                     /locus_tag="BJ961_RS30290"
     CDS             complement(4151..5017)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="BJ961_RS30290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase family protein"
                     /protein_id="WP_271415960.1"
                     /transl_table=11
                     /translation="MAYTRKDVSTLTRTEKRRFVGALLEIKRRGEYDEFVRTHIEYYVS
                     DGEDGLRTAHMAPSFLPWHRRFLLDLEEALRRVDSSVTVPYWDWTKDRTARSVPWTADL
                     LGGTGRRSDHRVTTGPFAHAEGNWTIKENVTDTEYLTRDLGRAGDPVDLPSRSDLEGAL
                     DDPEYDTSPYDSTVRKGFRNKLEGWGAGRGSVSWRNHNRVHRWVGGAMVGGASVNDPVF
                     WLHHAFIDLQWSRWQARHRGARYLPAEPPGRGSAQRGRIVARHEKLPPWDVTPDELEDV
                     GRIYRYA"
     gene            complement(5001..5642)
                     /locus_tag="BJ961_RS30295"
     CDS             complement(5001..5642)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325460.1"
                     /locus_tag="BJ961_RS30295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_271415961.1"
                     /sec_met_domain="melC (E-value: 1.3e-45, bitscore: 153.7,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVDADRNAGAHAATRDAEHERGREVEREGAAAGGPVPGTRRQLM
                     RGLLAPALAVGLAPLVAASGPTRAAEAAAGRPGAGRAHPGPHTAESIESSDSAAAVDSP
                     GSAGSAAGTRESTTFDETYRGRRIRGIRSAAGRAVGAGTWHVTVDGRPLHLMRRADGSW
                     LSMVDHYRSYATPLDAARGAVDELGPGEHLRDTPSTGHDQHSGGRHGVHA"
     misc_feature    complement(5617..5628)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
     gene            complement(5767..6282)
                     /locus_tag="BJ961_RS30300"
     CDS             complement(5767..6282)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976098.1"
                     /locus_tag="BJ961_RS30300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_271415962.1"
                     /transl_table=11
                     /translation="MKPTTRRTLAAVVTGVAAAVGGSATPAAAAGAIPVPVPLGGAETA
                     LGMELPEVAGELPVPTAGAPEGPRYVEGRLLPERTLPQLPVHGGLPGADLRQPLPHLLG
                     DDFDHAGVDAPAAGLRTLTPGLSLDAPLTAPDPAALGLPAPKLPQAGVLAPVVRTVPVA
                     DLGVGPGL"
     gene            complement(6367..6657)
                     /locus_tag="BJ961_RS30305"
     CDS             complement(6367..6657)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445910.1"
                     /locus_tag="BJ961_RS30305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_271415963.1"
                     /transl_table=11
                     /translation="MKSLKAAAVVAGSLVAAGSAVPAFAYDAADTAALTPTSLNGAVNA
                     LTAEPIDIMPLKHQSDALDTENKDSVLHTVKGATTELNSASGLLGGLPLGG"
     gene            complement(6869..7651)
                     /locus_tag="BJ961_RS30310"
     CDS             complement(6869..7651)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325463.1"
                     /locus_tag="BJ961_RS30310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_271415964.1"
                     /transl_table=11
                     /translation="MRLPARRIATTALCAGLLTGLSGPAVMAADGGPARERTHAASRAP
                     LPDAEELQSQVADLAGLGGMLTPVTDMLTAVLKADNGQLSATDADKLSAAVKDALAKAD
                     AANPADTADPDADDAATAPGTTTPAQPPAVTAPEAGTGTPVTLPAPVTAQETDGTAAAS
                     DLSATAQTALQKQIDALVKATTSGSAEQVSPAVEDVMTGIVNVVAATLVGNGLPAADLA
                     GLPGVPTAPEATTPEATAPEAAAPEAAVPANPLPADPS"
     gene            complement(7926..8165)
                     /gene="chpG"
                     /locus_tag="BJ961_RS30315"
     CDS             complement(7926..8165)
                     /codon_start=1
                     /gene="chpG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449696.1"
                     /locus_tag="BJ961_RS30315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="chaplin ChpG"
                     /protein_id="WP_271415965.1"
                     /transl_table=11
                     /translation="MSRIAKGLALTSVAAAAVAGTAGVAAADAGAQAAAAHSPGVLSGN
                     VVQVPVHIPVNVCGNTIDIIGLLNPAFGNECEND"
     gene            8427..9812
                     /locus_tag="BJ961_RS30320"
     CDS             8427..9812
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325465.1"
                     /locus_tag="BJ961_RS30320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycosyltransferase"
                     /protein_id="WP_271415966.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 2.1e-31,
                     bitscore: 107.6, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHLPPTGLRPRVLHITQPADGGVARVVTELTRAQLTAGLGVVVAC
                     PDGSLAERLRALGADVRHWSATRSPGPALVPEVRRLARVVEDVRPDLVHAHSAKAGLAG
                     RLAVRGRIPTVFQPHAWSFEAVGGPAAVLALGWERWAARWAARTVCVSEAERLRGVRAG
                     IRGPATVIPNGVDLDRFHTDAPGTPDVPNAPNTPDAPGLPDAPDVPEGPGASPGPARVR
                     QRHGVAPHAPLVVCVGRLCRQKGQDVLLDAWEPVLARVPGARLVLVGDGPDRARLAARA
                     PASVLFAGAVADAAVWYRAADLVVLPSRWEGMALAPLEAMACGRPVVVTDVDGAREGLP
                     PALVPHCLVPPEDPAALADAVSALLLDTPLRASLGRRGRAHVRSGHDVRHTSAAVTALY
                     GDLLGGVHRAPAAGPAPGATPLAHGAPSTEAPAAPRPSPGDRPAGVGRPAVVPIECRES
                     IQP"
     misc_feature    8448..8450
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            9809..10150
                     /locus_tag="BJ961_RS36245"
     CDS             9809..10150
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BJ961_RS36245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_408648710.1"
                     /transl_table=11
                     /translation="MSAESTVPSPAGQSRELGPSPVSVLPQRDGTGTPGCPPGVRAAAG
                     LPAAAARRGRHGGPAGLTGPHRRPHQPLLAALLVAASLLLRPGRTRPGGRAARWTTCPP
                     CAAGSRWSG"
ORIGIN
        1 cgtgctcgtc gagcagccgc ccgccccggt cgcgcagccg cagcgcgccg tgggcgccga
       61 gccgctcggc gagccgctcc ccggcgacgc gggcacgccg gccgcccagg agcacggtgg
      121 cgaccaggcc ggccaccacc ccggggtccg gcgcgaggtt gcggtccagc tcgcgcacct
      181 cctcctcggc gtactcctcc aactcgcgcc gccaccgccg tacggccatc ccgatgcggt
      241 gctcggcgct ctccggcgcg ggagcgcgct ccgcgagttc gggggcggcc gccgccggtt
      301 cgcgccgcca ggcctcgtcg acgtgttcgt cggcggcggt gacggcgcac agcagcagcg
      361 cgctcaggct ctccaccagc gagtcgagca gttcctccgg ggagcagtcg agggggaagg
      421 cccgccagcg cttcagcgcg tccccggcga gtacggcgcc cgcctgcaac cgcccccgca
      481 cgcgcgcgtg ctcgccgtcg taggcgccct cgacggcggc ggtgagccgc agcgcggcgg
      541 cgtactgcgc ggcggtggca ccggccagct cgggcatccg ggacttgagc gagtcgagga
      601 tgccgtgcgc ggtgcgggcc atggcgtggt gccgggcctc gagttcctgg gcccggtgga
      661 cgagccagga ccgcagcggc gccacggcgc tggccggcag cagaccaccg ccccaggccg
      721 actcgggcag ttcggggacc gtgaagcggg ggacgtcgcc gagcccggcc ttggtgagca
      781 gtgccccgta ctgccgcgac acctcggaca ccacctggtg gggcacccgg tcgagcacgg
      841 tgaccagggt ggcgtcgtac tccttggcgg tgcgcagcag gtgccagggc accgcgtcgg
      901 cgtaccgggc ggccgtggtg accatcaccc agatgtcggc ggcgcagagc agttcggcgg
      961 ccaggacgcg gttgtcggcg acgagggagt cgatgtcggg tgcgtccagc agggcgatgc
     1021 cgggcggcag ggtgtcggcg gtctcgaccc gcagcacgcg cgcggggttc tcgccgggca
     1081 gcagcaggtc gtcgtccggg tcccggtggg gcacccacac gcgcgtgagg tcgggcagca
     1141 cccgcatgcc gctgaaccag tggtggtcct ccggatggca gaccaggacc ggcgtacggg
     1201 tcgtgggccg cagcacaccc gcctcgctga cccggcggcc cacgagggag ttcaccaggg
     1261 tcgacttccc ggcgccggtg gatccgccca ccacggcaag caacggcgct tcgggctctt
     1321 tcaggcgggg caccaggtag tcgtcgagct gtgcgagcag ttcgtcgcgg ttggcacgcg
     1381 cgcgtggcgc ccccgccagg ggcagcggga agcgtgcggc cgcgacacgg tcgcgcaggg
     1441 cggagagtgc gtcgagcagc tgaggccgta cgtccaaggt caccacatgg gaagaatgcc
     1501 caattttagg ggaattctga agcatatgag catgatgagc atgtctgcgc gccgaccgga
     1561 cactccggac ggaagggact actggggcgc gaggacgggc caggcataac gagtggacaa
     1621 cacccgatgc gctggaggcc aaaagcgatg cacgattcgt acctgcctgc gattatcagg
     1681 accgcttcac cgaacctcca catcgagcca cggaggggaa gcatcaggga catggagccg
     1741 ggagccctat ccttgtcccc ggcgacgtca cggaccggcc cgcacccggg ccgtacgacc
     1801 gaggccacca caccaggccc ccgtagctca gtggatagag caggcgcctt ctaagcgctt
     1861 ggccgcaggt tcgagtcctg ccgggggcgc cactgcccgt ttggtgggag ggaacggacg
     1921 acgtgcgact gcgctgcgcg gtgctcgacg acttccagcg ggtggcgacc gagatcgccg
     1981 actggtcccc ggtcgcgggc gaggtggacg tcgtgtcctt cgacacgcat ttcgccgacg
     2041 aggacaccct cgccgcggcg ctcgccgacg tcgacatcgt ggtcaccctg cgcgagcgcg
     2101 tgtccttccc cggctcgctg atcgcccggt tgccgcggct gcggctgatc gtcgcctcgg
     2161 gcatgcgcaa cggcgtgatc gactacgccg ccgccgaggc gcacggcgtc accgtctgcg
     2221 gtacggcctc ctcctcgacc ccgcccgtcg aactgacctg ggccctgctg ctcggcctgg
     2281 cgcgcggcat cgtccaggag ggcaacggac tgcgcgggtc ggggccctgg cagcagacgg
     2341 tgggcgccga tctgcacggc cgcacactcg gactgctggg gctgggcagg atcggcggcc
     2401 gggtggcgcg ggtcggcctc gccttcggca tgcgggtgag cgcctggagc cagaacctca
     2461 cccgggagcg tgccgaggag gtcggtgtcg aacgggcccc gtccaaggaa gagctgctga
     2521 ggaccgccga cttcgtctcc gtccacctgg cactgggcga gcgcacccgt gggctgctgg
     2581 gccccgccga actggccctg ctcaagccga ccgcctacct ggtcaacacc tcgcgcgcgg
     2641 cgatcgtcga ccagggggcg ctgctcgccg cgctgcacga gggccgtatc gccggcgcgg
     2701 gcgtggacgt cttcgacatc gagccgctgc ccgccggtca tccgatgcgc agcgccccgc
     2761 ggctgctcgc gacgccccac ctcggctatg tctcgcgcgc caactacgcc acgtactacg
     2821 gccaggccgt cgaggcgatc gcggcctacc tggcgggctc ccccgtacgg cgcctgcccg
     2881 tgccggcggc cccttcccag ggcccggtgc cgggaccggc cgggtcaccg gcaccgacca
     2941 tgtcgtaggg acgctcaggc ggtgcccgtg cgggcggtgt ccgcggggcg cgcgtagatg
     3001 ccgtcgctcc gacgcaggtg accgaagtcc accaggtggc gccggagcgt cacatggtcg
     3061 acctcgccgt cctcgcacca cgcccgcagc ttctcgtcga cggtgcgctc cggatactcc
     3121 acgccgggcg cgaaggtctg ttcggcgatg tgccgcagca ccaccttctt gcgcgtccag
     3181 cgcgcgggca ggcgcaccag ccgcccgtcc ctgacgaagg tccgcacgat catgtcggcc
     3241 gggccgtccc cgctcccccc ggcgggttcc ggggccgccc cgcgcaccga ccggccggtg
     3301 cgggccagct cccggaaacg gtcgtgtgcg acgacgagtc cgccgtcgtc cccggacgac
     3361 accacgccct gctcccgcag ccggcgcagc gcaccggccg tctccttcgc ggagaggccg
     3421 gccgccaggg ccaccccgcg gcgtcgcccg cgcccagggc cacggccgcg aaggcccgca
     3481 cgcggctctc ctcggcgaag agagcgggca gcgcggccag cgcggacggc acggcgggcc
     3541 cggacgacgg ctcggacgac ggctcggacg gctcggacgg ctcggacgac ggctcgggag
     3601 cctcggcgga cgagtcggat cggtcattcg gcatgccggg aggctagtgg acgcaccggt
     3661 gcgcacggga atggttttcc gctgccggcc ctccccgttc aaaacgggtg atccacacat
     3721 tccccccgtg tcccgccgcg accgagtgcg ttgaaccggc agtgcggtgg cgcgtcctgc
     3781 caccgtgctc cccgagtcag aaggagaacg tgatgtctcg catcgcgaag gccgccggag
     3841 tcgccctcgg cgccggtgcc gtggcgctca gcggcgccgg cctggccatg gcggacgcgg
     3901 gcgcgcaggg tgcggccgtc ggctcgcccg gtgtcctgtc cggcaacgtc gtccaggtcc
     3961 cggtgaacgt gccggtcaac gtctgcggca acaccatcga cgtgatcggc ctgctgaacc
     4021 cggccttcgg caacgcctgc gaaaacggcg acgaccacga cgacaagagc ggtggctacg
     4081 gcggctgagc ccccaggcgc ccgaggagcc ccggccttcc aggccggggc tcctcccgta
     4141 cccgccccgc tcaggcgtac cggtagatcc ggccgacgtc ctccagttcg tccggcgtca
     4201 cgtcccacgg cggcagcttc tcgtgccgtg ccacgatccg cccgcgctgc gcgctgcccc
     4261 ggccgggcgg ctcggcgggc aggtagcggg cgccgcggtg gcgggcctgc cagcgggacc
     4321 actggaggtc gatgaaggcg tggtgcagcc agaacaccgg gtcgttgacg gaggcgccgc
     4381 cgaccatggc cccgccgacc cagcggtgca cgcggttgtg gttgcgccag gagacgctgc
     4441 cgcggccggc gccccacccc tccagcttgt tgcggaatcc cttgcggacc gtggagtcgt
     4501 agggcgacgt gtcgtactcc gggtcgtcca gcgccccttc caggtcgctc ctggagggca
     4561 ggtcgacggg gtcgccggcg cgtccgaggt cccgggtgag gtactcggtg tcggtgacgt
     4621 tctccttgat ggtccagttg ccctcggcgt gggcgaaggg gccggtggtg acccggtggt
     4681 cggagcgccg cccggtgccg ccgaggaggt cggcggtcca gggcaccgac ctggcggtgc
     4741 ggtccttcgt ccagtcccag tacggcacgg tcaccgacga gtcgacccgg cgcagcgcct
     4801 cctccaggtc cagcaggaac ctgcggtgcc agggcaggaa ggacggcgcc atgtgcgccg
     4861 tacgcaggcc gtcctcgccg tcggagacgt agtactcgat gtgggtgcgc acgaactcgt
     4921 cgtactcgcc gcgccgtttg atctccagca gcgcgcccac gaaccgtcgc ttctcggtac
     4981 gggtcagcgt gctgacgtcc ttacgcgtgt acgccatgcc gccccccgga gtgctggtcg
     5041 tggccggtgg acggcgtgtc gcgcaggtgc tcgccggggc cgagttcgtc gacggcgccg
     5101 cgggccgcgt ccagcggggt ggcgtacgag cggtagtggt cgaccatgct cagccagctg
     5161 ccgtcggccc ggcgcatcag gtgcagcggt cggccgtcca cggtgacgtg ccaggtaccg
     5221 gcgccgacgg cccgtcccgc ggcggaacgg atgccgcgga tgcgccggcc gcggtaggtc
     5281 tcgtcgaagg tggtgctctc gcgagtgccg gcggcgctcc cggcggaacc aggagaatcg
     5341 acggctgcgg cggaatcgct ggattcgatg gattcggcgg tgtgcggtcc ggggtgtgcg
     5401 cgccccgccc ccggcctgcc ggcggcggcc tccgcggccc gggtgggccc ggaggcggcg
     5461 accaggggcg ccaggccgac ggcgagggcc ggggcgagca gcccccgcat cagctgccgc
     5521 cgggtaccgg gcaccggccc gcccgccgcc gcgccctccc gctccacctc gcgtccgcgc
     5581 tcgtgctccg cgtctctcgt tgccgcgtgc gcgcccgcgt tcctgtccgc gtcgacgacc
     5641 atggctcgct ccttgtccgg ttccggtggt ggtccgcacc gctaaccgca cgacgggccc
     5701 cacggtcacc gctcgcaggg ccagtcggag cacggccgtc tcgccgggcg gggtcgtcgc
     5761 gccggactac agccccggtc cgacgcccag gtcggccacc ggaaccgtcc ggaccaccgg
     5821 cgccagtacc ccggcctgtg ggagcttggg tgccggcagg ccgagggccg cggggtccgg
     5881 cgcggtgagc ggggcgtcca gggaaaggcc gggcgtcagg gtccgcagcc cggcggcggg
     5941 ggcgtcgaca ccggcgtggt cgaagtcgtc gccgagcagg tgcggcagcg gctggcgcag
     6001 gtcggcgccg ggcagcccgc cgtgcaccgg caactgcggc agggtccgct ccggcagcag
     6061 ccggccctcg acgtaccgcg gtccctcggg ggcgccggcc gtcggcacgg gcagctcgcc
     6121 cgccacctcg ggcagctcca tgccgagggc cgtctccgcg ccgcccagcg gcaccggcac
     6181 ggggatcgcg ccggccgcgg cggccggggt cgcggacccg cccacagcgg cggccacacc
     6241 ggtgacgacg gcggccaggg ttcgtctggt ggtcggcttc atgacaggca cggtcccttt
     6301 cgagaatgct ggaagaacga cgagaacgac gagaacgacg agaacgacga cgaggtgcgg
     6361 ggttgttcag ccgcccagcg gaagtccgcc cagcaggccg ctcgcggaat tcagttcggt
     6421 ggtcgcgccc ttcaccgtgt gcagcacgga atccttgttc tcggtgtcca gcgcgtccga
     6481 ctggtgcttg agcggcatga tgtcgatggg ctccgcggtc agcgcgttca cggcaccgtt
     6541 gaggctggtg ggcgtgagag cggcggtgtc ggcggcgtcg taggcgaacg cgggcacggc
     6601 ggagccggcg gctaccaggg aaccggcgac gacggcggcg gccttgaggg acttcatcgt
     6661 gttcctttct tcggcgagcg tgtcaccgga ggaattgctg acgccctccc taacgacctg
     6721 cgccggaccc ggaaactccg ccgtccggaa gacatatgag atctccggcc gcgcagcctg
     6781 tccgccggaa gggtgcctcg cgagaacgag gaacgggccg ccgcgcctcc gggaggggag
     6841 gtacgacggc ccggaacgcc ccggggcgtc aggagggatc ggccggcagc gggttcgccg
     6901 gtacggcggc ttccggcgcg gccgcctcgg gcgcggtggc ctccggggtg gtggcctccg
     6961 gcgcggtcgg tacgccgggc aggccggcca ggtccgcggc gggcaggccg ttgccgacga
     7021 gcgtggcggc gacgacgttg acgatgccgg tcatgacgtc ctcgacggcc gggctcacct
     7081 gctcggcgga gccggaggtg gtggccttga ccagggcgtc gatctgcttc tgcagcgcgg
     7141 tctgggcggt cgcggacagg tcggacgccg ccgccgtccc gtcggtctcc tgcgccgtga
     7201 cgggcgccgg cagggtgacg ggggtgccgg tcccggcctc cggcgcggtc acggccggcg
     7261 gctgggccgg ggtggtcgtg cccggggcgg tcgccgcgtc gtcggcgtcc gggtccgcgg
     7321 tgtccgccgg gttcgccgcg tccgccttgg cgagggcgtc cttgacggcg gcggagagct
     7381 tgtccgcgtc cgtggcggag agctggccgt tgtcggcctt gaggacggcg gtgagcatgt
     7441 cggtgaccgg cgtgagcatc ccgcccaggc cggccaggtc ggcgacctgg ctctgcagtt
     7501 cctccgcgtc gggcagcggc gcgcgggacg ccgcgtgggt gcgttcccgg gccgggccgc
     7561 cgtcggccgc catcaccgcc ggaccggaga ggccggtcag gaggccggcg cagagtgcgg
     7621 tggtggcgat tcgccgtgcg ggcagacgca tgggggttcc tttcggtggt gcgtggatct
     7681 ttcgcccacc gtggaagcgc ccccgacgct ctgcaaccga gcggtgaccc ggcatgctcg
     7741 cccgcctctc acccggcccg cgtcctgcct ggtgggacac cctgtcaaga cgcgtccgcc
     7801 gtgtcggcgc caggtccccc cattcggggc agcacaccgc cgtccgtgcg gcaacgcgaa
     7861 ccggccgccc gctcgcggag gaaaccgcgg tacggacggc cggtgcgggg agttgccggt
     7921 cgacgtcagt cgttctcgca ctcgttgccg aacgccgggt tcagcaggcc gatgatgtcg
     7981 atggtgttac cgcagacgtt gaccgggatg tggaccggga cctgcaccac gttgccggac
     8041 aggacgcccg gggagtgggc ggcggcggcc tgcgcgccgg cgtcggcggc ggcgacaccg
     8101 gcggtgcccg ccaccgcggc ggcggcgacg gaggtcaggg ccaggccctt cgcgatacgc
     8161 gacatggaga agtgctcctt gggggttgtc acacacatcc gggcggctgc ccggcgcagt
     8221 gtcaacgcgt ggcgcccgcg cgagttgtgc cgccaaaggg gtgatctccc cggaaaccgg
     8281 cattcacaaa gtcaacacac ttacccggtt tcaccgcacc agtagggata gcggttagag
     8341 tcccacaccg gtcaagacat cccttttggg ccgcttcgca ccagcatttc ccgtcccgag
     8401 ccttctccgt gagcgaggaa acgcgcatgc acctgccacc gaccggatta cggccgcggg
     8461 ttctgcacat cacccagccg gcggacggcg gggtcgcccg ggtggtgacg gagctgacgc
     8521 gggctcagct caccgcgggg ctgggcgtcg tggtcgcctg tccggacggg agcctggccg
     8581 agcggctgcg tgcgctgggc gccgacgtgc ggcactggtc cgcgacgcgc tcgccgggcc
     8641 cggccctggt gccggaggtc cgccggctcg cccgcgtggt cgaggacgta cggcccgacc
     8701 tggtgcacgc ccacagcgct aaggccggtc tcgcggggcg gctcgccgta cggggccgga
     8761 tcccgaccgt gttccagccg cacgcctggt cgttcgaggc ggtcggcggg cccgccgcgg
     8821 tcctcgccct cggctgggag cggtgggcgg cgcggtgggc ggcgcggacg gtgtgcgtga
     8881 gcgaggcgga gcggctgcgg ggcgtgcggg ccggcatccg cgggcccgcg acggtcatcc
     8941 cgaacggcgt cgacctggac cgcttccaca ccgacgcccc cggcacaccg gacgtcccca
     9001 acgccccgaa caccccggac gcccccggcc tccccgacgc gcccgacgtc cccgaaggcc
     9061 ccggtgcctc ccccggcccg gcccgcgtac ggcagcgcca cggcgtcgcc ccgcacgccc
     9121 cgttggtggt gtgcgtgggg cggctgtgcc ggcagaaggg gcaggacgtc ctgctggacg
     9181 cgtgggagcc ggtcctcgcg cgggtgcccg gcgcccggct ggtcctggtc ggcgacggcc
     9241 ccgaccgtgc ccggctggcc gcgcgggccc cggcgtccgt gctgttcgcg ggcgccgtcg
     9301 cggacgccgc cgtctggtac cgggccgccg acctggtcgt cctgccgtcg cgctgggagg
     9361 gcatggcgct ggccccgttg gaggcgatgg cgtgcgggcg gcccgtggtg gtgaccgacg
     9421 tggacggtgc ccgcgagggc ctgccgcccg ctctcgtgcc gcactgcctg gtaccgcccg
     9481 aggatcccgc ggcactggcc gacgccgtga gcgcgctgct gctcgacacg ccgctgcgcg
     9541 cgtcgctcgg ccgccggggg cgcgcacacg tccggtccgg acacgacgtg cggcacacgt
     9601 ccgcggcggt cacggccctg tacggcgacc tcctcggcgg cgtacaccgc gcaccggccg
     9661 ccggtcccgc ccccggggcc acgcccctcg cgcacggcgc tccgtccacc gaggcgcccg
     9721 ccgcgcctcg tccctccccc ggcgacaggc ccgcaggtgt ggggcggccg gccgtcgtgc
     9781 ccatcgagtg cagggagtcc atccagccgt gagcgcggaa agcaccgtcc cctcccccgc
     9841 cggccagtcg cgcgaactcg gcccgtcccc cgtctccgtg ctgccgcagc gcgacggcac
     9901 cggcaccccc ggctgcccgc cgggcgtccg cgccgcggcc ggactccccg ctgccgctgc
     9961 tcgtcgcgga cggcacggcg gccctgccgg gctcactggc cctcaccggc gcccccacca
    10021 gccgctgctc gcggccctgc tggtcgcggc ctcgctgctg ctgcgccccg gccgcacccg
    10081 gcccggcggc cgtgcggcgc gctggacgac ctgcccgccc tgtgcggccg ggtcgcggtg
    10141 gtctggctga cgctggcggc gctcgtcgcg gcgtactccc cgccgcaggc gctgtccgtg
    10201 cgcaccctgg tcctcggctt cctgctgcac acggcggcgg gctgcgccgg ccgggccgcc
    10261 gtgcaccgca ggcaccgtac ggcgctgctg agccgccccc gcgcggccct cgtcgtcggt
    10321 ccggcggcga cggcgcagcg cgtggcctcg gcgctgctgc gccacccgcg ctgcggactc
    10381 cgcccggtcg gggtcgtcac cgacgaaccg ggcggcgccg cgggcctgcc cgtgctctcc
    10441 acgggcgagg aggcgcggcg ggcggtcgtg cagaacggcg tacgggacgt cctgatcgtc
    10501 gccccctctg ccgccccgtc cgctgccccc tccgtgcctc cctccgtgga ccccgccgtt
    10561 cggacccggc aggcggcgct gctgcgggcg ctggccgggg cgggctgcgc ggtgtgggag
    10621 gtggacgcgg agaccccctc cc
//
