LOCUS       NZ_CP116257            10399 bp    DNA     linear   CON 15-DEC-2024
DEFINITION  Streptomyces lienomycini strain DSM 41475 chromosome, complete
            genome.
ACCESSION   NZ_CP116257
VERSION     NZ_CP116257.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces lienomycini
  ORGANISM  Streptomyces lienomycini
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7863969)
  AUTHORS   Ding,W.
  TITLE     Streptomyces riduolalong sp. nov., isolated from the soil of Tibet
            exhibits against Gram-positive bacteria activity
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7863969)
  AUTHORS   Ding,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) School of life sciences and biotechnology,
            Shanghai Jiaotong University, 800 Dongchuan, Shanghai, Shanghai
            200240, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: GS De Novo Assembler v. August-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_027947595.1-RS_2024_12_13
            Annotation Date                   :: 12/13/2024 16:03:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,183
            CDSs (total)                      :: 7,093
            Genes (coding)                    :: 6,850
            CDSs (with protein)               :: 6,850
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 243
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 243
            Pseudo Genes (frameshifted)       :: 141 of 243
            Pseudo Genes (incomplete)         :: 121 of 243
            Pseudo Genes (internal stop)      :: 14 of 243
            Pseudo Genes (multiple problems)  :: 26 of 243
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:34:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5714068
            Orig. end                         :: 5724467
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116257.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="26"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(850..1356)
                     /locus_tag="BJ961_RS26070"
     CDS             complement(850..1356)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028017.1"
                     /locus_tag="BJ961_RS26070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_271415226.1"
                     /transl_table=11
                     /translation="MPALLLLVLVALVTAVVLRRGAGTYPRRPVAAVPAAAVAGPRGPG
                     APFRILAGAAGGAAVLLYLWGALCVGLAVMQAEDGGAGSSPPLPCRAGVSPELSGTVVG
                     HSASYLPFGFRCETLDGDGYASADIPGYLNPGVAVLALTAVGGAVAAGFATERQARAAA
                     RGDGR"
     gene            1535..2662
                     /locus_tag="BJ961_RS26075"
     CDS             1535..2662
                     /NRPS_PKS="Domain: Aminotran_5 (19-291). E-value: 1.1e-27.
                     Score: 88.8. Matches aSDomain:
                     nrpspksdomains_BJ961_RS26075_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976957.1"
                     /locus_tag="BJ961_RS26075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="WP_271415227.1"
                     /sec_met_domain="Aminotran_5 (E-value: 2.4e-20, bitscore:
                     71.5, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLSAGRFAAIEDRVARLLGTAQDVVVMQGEALLPLE
                     GAIRAAAGPGTVALNIVTGPYGQTFGNWLRDCGATVHDLAVPFHAAVSAEQVRRAFAEH
                     PEIDFVSLVHAEAATGNTNPVAEVGEVVRAHGALFYLDAVASVGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWARMAANPNAPRRSYLSLLDWKERWIDGGRKALPHAP
                     AQLEMLALQACVERFEAEGAGTVMARHASAAAATRAGALALGGLEPYVYEARDAAPVAT
                     TLRTPSDVDASALVARALESDPALPLAAGGGALAGEMIRVNHYGPDATRGAVQGCLAAL
                     GAALAERGLKADLEAARRAVEEAWERPAGTGLSEG"
     aSDomain        1592..2407
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BJ961_RS26075_Aminotran_5.1"
                     /evalue="1.10E-27"
                     /label="BJ961_RS26075_Aminotran_5.1"
                     /locus_tag="BJ961_RS26075"
                     /protein_end="291"
                     /protein_start="19"
                     /score="88.8"
                     /tool="antismash"
                     /translation="EDRVARLLGTAQDVVVMQGEALLPLEGAIRAAAGPGTVALNIVTG
                     PYGQTFGNWLRDCGATVHDLAVPFHAAVSAEQVRRAFAEHPEIDFVSLVHAEAATGNTN
                     PVAEVGEVVRAHGALFYLDAVASVGAEPVLPDAWGVDLCVIGAQKAMGGPAGVSAVSVS
                     ERAWARMAANPNAPRRSYLSLLDWKERWIDGGRKALPHAPAQLEMLALQACVERFEAEG
                     AGTVMARHASAAAATRAGALALGGLEPYVYEARDAAPVATTLRTPSDVDA"
     misc_feature    complement(2811..2826)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    2821..2836
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    2955..2967
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    complement(2967..2982)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 16.79"
                     /tool="antismash"
     misc_feature    2981..2993
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     gene            2992..3504
                     /gene="ectA"
                     /locus_tag="BJ961_RS26080"
     CDS             2992..3504
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448158.1"
                     /locus_tag="BJ961_RS26080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_271415228.1"
                     /transl_table=11
                     /translation="MTAAQADLQIDRPRVADGAALWRIARDSEVLDLNSSYSYLLWCRD
                     FAATSAVARDERGEPVGFVTGYVRPDRPDTLLVWQVAVDAAYRGRRLAASLLDGLAERT
                     VAERGLTTLETTISPDNTASQRLFTSFAERRGARLEREVLFDTDVFPDGPHEPEVLYRI
                     GPLTATG"
     gene            3630..4901
                     /gene="ectB"
                     /locus_tag="BJ961_RS26085"
     CDS             3630..4901
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-366). E-value: 1.1e-72.
                     Score: 236.8. Matches aSDomain:
                     nrpspksdomains_BJ961_RS26085_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="BJ961_RS26085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_271415229.1"
                     /sec_met_domain="Aminotran_3 (E-value: 5e-76, bitscore:
                     254.7, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRGWPTVFDRAVGSRMYDEDGHEY
                     LDFFAGAGSLNYGHNNAVLKRALIDYLERDGVTHGLDMSTTAKRRFLETFQNTVLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GVPLVHGTPMPFDNYFDGTVEDFIWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALAELCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGLPMA
                     LTLFKPELDVWEPGEHNGTFRGNNPAFVTATATLEAYWADGSAMEKQTRKRGEQVEQHM
                     IAITEENLADVKEYRGRGLVWGLEFHDKGRAGRVAKRAFELGLLIETSGPESEVVKLLP
                     ALTITPDELDEGMKTLARAVRETA"
     aSDomain        3717..4727
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BJ961_RS26085_Aminotran_3.1"
                     /evalue="1.10E-72"
                     /label="BJ961_RS26085_Aminotran_3.1"
                     /locus_tag="BJ961_RS26085"
                     /protein_end="366"
                     /protein_start="29"
                     /score="236.8"
                     /tool="antismash"
                     /translation="DRAVGSRMYDEDGHEYLDFFAGAGSLNYGHNNAVLKRALIDYLER
                     DGVTHGLDMSTTAKRRFLETFQNTVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKGR
                     ESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGVPLVHGTPMPFDNYFDGTVEDFIWFERL
                     LEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALAELCERQDMLLIVDDIQMGCGRT
                     GAFFSFEEAGITPDIVTVSKSISGYGLPMALTLFKPELDVWEPGEHNGTFRGNNPAFVT
                     ATATLEAYWADGSAMEKQTRKRGEQVEQHMIAITEENLADVKEYRGRGLVWGLEFH"
     gene            5001..5399
                     /locus_tag="BJ961_RS26090"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037840.1"
                     /locus_tag="BJ961_RS26090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_217162780.1"
                     /sec_met_domain="ectoine_synt (E-value: 5.4e-54, bitscore:
                     180.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKEFEGTDRHVKSASGTWESTRIVLAKEKVGFSVHETILY
                     AGTETSMWYANHIEAVVCTKGDAELTDRETGKTYHITPGTMYLLNGHERHTLKVKEDFH
                     CICVFNPPVTGREDHDENGVYPLLTEEV"
     gene            5406..6305
                     /gene="thpD"
                     /locus_tag="BJ961_RS26095"
     CDS             5406..6305
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976953.1"
                     /locus_tag="BJ961_RS26095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_271415230.1"
                     /sec_met_domain="PhyH (E-value: 7.4e-39, bitscore: 132.9,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTTTNVTDLYPTRGATEVATPRQDPVVWGSPDTPGPVSAGDLQ
                     SLDRDGFLAIDQLIGPDEVGVYQRELERLVGDPAIRADERSIVEPQSKEIRSVFEVHKI
                     SEVFAKLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     NMRTISVSIALTENYDTNGGLMIMPGSHKTFLGCAGATPKDNYKKSLQMQDAGTPSDEG
                     LTKLASEYGIKLFTGKAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENTAVEPFA
                     APIRRPDFVGARDFTPVR"
     gene            complement(6384..7478)
                     /locus_tag="BJ961_RS26100"
     CDS             complement(6384..7478)
                     /NRPS_PKS="Domain: Aminotran_5 (28-358). E-value: 5.5e-22.
                     Score: 70.1. Matches aSDomain:
                     nrpspksdomains_BJ961_RS26100_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448151.1"
                     /locus_tag="BJ961_RS26100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_271415231.1"
                     /sec_met_domain="Aminotran_5 (E-value: 9.5e-16, bitscore:
                     56.4, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDGEVRGPHSGAMGSFENLVRTEFAPKTTYLNTASTGLLPARTLV
                     AMRDAAAFAADGQPLDMFADVEAARAAYARLVGVPATRVATGASVATFSGLVAASLPPG
                     AEVLTAEGDFSSLVNPLYTRGDLKVRSVPLELLAESVGPDTALVAVSSVQSADGRVADL
                     AALRDAARAHGARTLIDVSQANGWLPTAADDFDYVTAVGYKWVLCPRGVAFLVVPQDLG
                     AAAGLTPLFAGWATGEHPWDSCYGPVGELARSARRFDESPALFSYTGARHSLALIEELG
                     VDAVHAHDTALADRFRAGARSLGHEPVPAPGSAIVSVPGLGHRQGELAGAGVQVSDRAG
                     NLRAAFHLYNTAADVDRLLDVLAG"
     aSDomain        complement(6405..7394)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BJ961_RS26100_Aminotran_5.1"
                     /evalue="5.50E-22"
                     /label="BJ961_RS26100_Aminotran_5.1"
                     /locus_tag="BJ961_RS26100"
                     /protein_end="358"
                     /protein_start="28"
                     /score="70.1"
                     /tool="antismash"
                     /translation="TYLNTASTGLLPARTLVAMRDAAAFAADGQPLDMFADVEAARAAY
                     ARLVGVPATRVATGASVATFSGLVAASLPPGAEVLTAEGDFSSLVNPLYTRGDLKVRSV
                     PLELLAESVGPDTALVAVSSVQSADGRVADLAALRDAARAHGARTLIDVSQANGWLPTA
                     ADDFDYVTAVGYKWVLCPRGVAFLVVPQDLGAAAGLTPLFAGWATGEHPWDSCYGPVGE
                     LARSARRFDESPALFSYTGARHSLALIEELGVDAVHAHDTALADRFRAGARSLGHEPVP
                     APGSAIVSVPGLGHRQGELAGAGVQVSDRAGNLRAAFHLYNTAADVDRL"
     gene            complement(7550..8263)
                     /locus_tag="BJ961_RS26105"
     CDS             complement(7550..8263)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448152.1"
                     /locus_tag="BJ961_RS26105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_271415232.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALAAFPHRDGVEVVHRSFELDP
                     GRAKDDVQPVLTMLTAKYGMSEAQARAGEDNLGAQAAAEGLDYRTRDRDHGSTFDMHRL
                     LHLAKARGRHEALLDAFYRGNFAEERSVFNDDGRLLELAVGAGLDAEEVRAVLADPGAY
                     ADEVRADEREAAQLGATGVPFFVLDRAYGVSGAQPAEVFSQALTQAWGERTPLKLIDDG
                     GAEACGPDGCAVPQK"
     gene            complement(8356..8964)
                     /locus_tag="BJ961_RS26110"
     CDS             complement(8356..8964)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="BJ961_RS26110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_271415233.1"
                     /transl_table=11
                     /translation="MTLELPELPFPLRPYGPDGRWSYADGVLTGVAGPRQDRFVPPTGE
                     ALESVSDAPRLLGAPDGDFQLIARVSVGFRGAFDAGVLYVHVGERAWAKLCLEYSPDAP
                     TVCTVVTRGHSDDCNSFTVEGDSVWFRVSRSGRAFAFHASLDGERWTFVRLFTLGDEAE
                     TGAALVGFLAQAPTGEGCTVTYDHLEFRPGWPADLRDGS"
     misc_feature    complement(9736..9751)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.08"
                     /tool="antismash"
ORIGIN
        1 cgaacggtgt cgtagcggcg ttcgtggagc cggcgcatgt gggcggacca gcgcgggaac
       61 cgggtctcgc caccggcggc cagcgtgggg aaggtggcga tgttgacgag ggtgggcacg
      121 atggcgacgc cgcgttcggc gaagagcggg atcgtgtcgt ccgtgagccc cgtggcgtgc
      181 tcgacgcagt cgatcccggc ctcgaccagg tcgcgcaggg agtcctcggc gaagcagtgc
      241 gcggtgacgc gggcgccgag gcggtgggcc tccgcgatgg ccttctcgac ggcgccgcgc
      301 ggccagcagg ccgacaggtc gcctaggtcg cggtcgatcc agtcgccgac gagtttcacc
      361 cagccgtcgc cgcgccgggc ctcctgggcg acgtacgcga cgaggtcctc gggctcgatc
      421 tcccaggcgt agttgcggat gtagcggcgg gtgcgggcga tgtgccggcc ggcccggatg
      481 atcttcggga ggtcctcgcg gtcgtcgatc cagcgggtgt cggagggcga gcccgcgtcg
      541 cggacgagga gggtgcccgc ctcccggtcg gtcagcgcct gcttctcggc gacgtcctcg
      601 gggacggccc cgtgctggtc caggccgacg tggcagtgcg cgtcgaccag gccgggcagc
      661 gcccacccct cgacggtgcg gacgtcggcg gcgcccgcgg ggcggtcgta cgagatccgg
      721 ccgtcgacca cccacaactc gtcccggacc tcctccggcc cgacgagcac ccgtcccctc
      781 acgtgcagca ccgcgcgatc gctcatgccc cgcaccctag accggggccc ggggctcttc
      841 gcggcccggt caccggccgt caccgcgggc cgcggcgcgg gcctgccgct cggtcgcgaa
      901 cccggccgcc acggcgccgc cgacggcggt gagcgccagg accgcgacgc cggggttgag
      961 gtagccgggg atgtcggcgg acgcgtagcc gtcgccgtcg agggtctcgc agcggaagcc
     1021 gaagggcagg taggaggcgg aatggccgac gaccgtcccg gacagctccg gggacacccc
     1081 ggcgcggcag ggcagcgggg gcgaggagcc cgcgccgccg tcctcggcct gcatgaccgc
     1141 gagcccgacg cacagcgcgc cccagaggta cagcaggacg gcggcgccgc cggcggcccc
     1201 ggcgaggatc cggaagggcg cccccggtcc gcgcggcccg gcgacggccg ccgccgggac
     1261 cgccgcgacg gggcggcggg ggtaggtgcc ggcgccccgg cgcaggacga cggccgtcac
     1321 cagcgcgacg aggacgagca gcagcagagc gggcacgggc gcaagtccat cagtccggca
     1381 cccacccggc tgtggcggaa gccacaattc cgggcgtggg ggtgtcgcaa cggaagccct
     1441 ccggcgcgag tgacggcggg ggccgtggtt actctcgact ccatacgcga cgtacccgcg
     1501 ccgcgcacct gccgttactg aagagagcac cgccgtgacc catcccttcc tggatctcgc
     1561 cccgctgagc gccggccgtt tcgccgcgat cgaggaccgg gtcgcgcgtc tgctgggcac
     1621 cgcgcaggac gtggtggtca tgcagggcga ggcgctgctg ccgctggagg gcgcgatccg
     1681 cgccgccgca ggtccgggca ccgtcgcgct gaacatcgtc acggggccct acgggcagac
     1741 cttcgggaac tggctgcggg actgcggcgc gaccgtgcac gacctcgccg tgccgttcca
     1801 cgcggccgtc tccgccgagc aggtccgccg ggcgttcgcc gagcacccgg agatcgactt
     1861 cgtgtcgctg gtgcacgccg aggcggcgac cggcaacacc aacccggtcg cggaggtcgg
     1921 cgaggtggtg cgggcgcacg gcgcgctgtt ctacctggac gcggtggcct ccgtgggcgc
     1981 ggagccggtg ctgccggacg cgtggggcgt ggacctgtgc gtgatcggcg cgcagaaggc
     2041 gatgggcggc ccggccgggg tgtcggcggt gtcggtgagc gagcgggcgt gggcgcggat
     2101 ggcggcgaac ccgaacgccc cgcgccggtc gtacctgtcg ctcctcgact ggaaggagcg
     2161 ctggatcgac ggcgggcgca aggcgctgcc gcacgcgccg gcgcagttgg agatgctcgc
     2221 gcttcaggcg tgcgtggagc gcttcgaggc ggagggcgcc ggcacggtga tggcgcggca
     2281 cgcgtcggcc gcggcggcga cccgggcggg cgcgctcgcg ctgggcggcc tggagccgta
     2341 cgtgtacgag gcgagggacg cggcgccggt cgcgacgacg ctgcggacgc cgtcggacgt
     2401 cgacgcgtcc gcgctggtgg cccgggccct ggagtccgac ccggcgctgc cgctggcggc
     2461 gggcgggggc gcgctggcgg gcgagatgat ccgggtcaac cactacggtc cggacgcgac
     2521 gcgcggggcg gtgcagggct gcctggcggc actgggtgcc gcgctggccg agcgggggct
     2581 gaaggccgac ctggaggccg ctcggcgggc cgtcgaggag gcgtgggagc ggccggccgg
     2641 caccggcctt tcggaaggat gaattcgagg gaattcccga agctcacaca agcagaattt
     2701 aggaattcca ctcagcgcag cttcccgtat tcttctgccc gctttccccg gacctaaaca
     2761 caaagatttc acgagtcccg atcttcacat ttcggacacg tctcgcacgg gttacgcgat
     2821 tgtgacccga tccacatgct gcccgttttg cggcatgtat cccgcacaaa acctctcata
     2881 acgccgccgt ctcgcgcggc agcacgcgcc cgcgtgataa cacatggggc gtccattcgt
     2941 aaccccatcc ggcgatgcaa tttcgaattt ccctgggtaa attcaatttg catgactgcc
     3001 gcgcaagcag acctgcaaat cgatcgtccg agagtggccg acggggccgc cctgtggcgg
     3061 atagcgaggg attccgaggt cctcgacctg aactcgtcct acagctatct gctctggtgc
     3121 cgggacttcg ccgccacgtc ggccgtcgcc cgcgacgagc gcggtgagcc ggtcggcttc
     3181 gtcaccggat acgtacgtcc ggaccgtccg gacaccctgc tcgtgtggca ggtggcggtg
     3241 gacgcggcgt accgcgggcg ccggctcgcc gcctccctgc tcgacgggct ggccgagcgg
     3301 accgtcgcgg aacgcggtct gaccacgctg gagaccacca tctcccccga caacacggcc
     3361 tcccagcggc tgttcacctc gttcgccgag cgtcgtggcg cccggctgga gcgcgaggtg
     3421 ctgttcgaca cggacgtgtt ccccgacggt ccgcacgagc ccgaggtcct ctaccgcata
     3481 ggtcccctga ccgcaaccgg ctgaccggcc gtccggacgg cccggcaccc cgtgccagtc
     3541 cggccccaga gacttcccgc ggaccgcacc ggtccccgga tccgtacgac cttcctctcc
     3601 caccctcacc ccacgaggag cgattcgtcg tgaccatcac ccagcccgac ctcagcgtct
     3661 tcgagaccgt cgagtccgag gtgcgcagct actgccgcgg ctggcccacc gtcttcgacc
     3721 gtgcggtcgg cagccgcatg tacgacgagg acggccacga gtacctcgac ttcttcgccg
     3781 gagcgggctc gctcaactac gggcacaaca acgccgtcct gaaacgggcg ctgatcgact
     3841 acctggagcg ggacggcgtc acgcacgggc tcgacatgtc gaccaccgcc aagcgccgct
     3901 tcctggagac gttccagaac accgtcctgc ggccgcgcga cctgccctac aaggtcatgt
     3961 tcccgggccc gacgggcacc aacgccgtgg agtccgcgct gaagctggcg cgcaaggtga
     4021 aggggcgcga gtcgatcgtg tcgttcacca acgccttcca cggcatgtcg ctgggctcgc
     4081 tggcggtgac cggcaacgcc ttcaagcggg ccggcgccgg cgtcccgctg gtgcacggca
     4141 cgcccatgcc gttcgacaac tacttcgacg gcaccgtcga ggacttcatc tggttcgagc
     4201 ggctgctcga ggaccagggt tccggtctga acaagccggc cgccgtgatc gtcgagaccg
     4261 tgcagggcga gggcgggatc aacgtcgccc gcgccgagtg gctgcgcgcc ctggccgagc
     4321 tgtgcgagcg ccaggacatg ctgctcatcg tcgacgacat ccagatgggc tgcggccgga
     4381 ccggcgcctt cttctccttc gaggaggcgg gcatcacgcc ggacatcgtc accgtctcca
     4441 agtcgatcag cggctacggc ctgccgatgg cgctgaccct gttcaagccg gagctggacg
     4501 tgtgggagcc gggcgagcac aacggcacct tccgcggcaa caacccggcc ttcgtcacgg
     4561 cgaccgccac gctggaggcc tactgggccg acgggtcggc gatggagaag cagacccgca
     4621 agcgcggcga gcaggtcgag cagcacatga tcgccatcac cgaggagaac ctcgccgacg
     4681 tcaaggagta ccgcggccgc ggcctggtct ggggcctgga gttccacgac aagggccgcg
     4741 cgggccgggt cgccaagcgc gccttcgagc tggggctgct catcgagacg tccgggccgg
     4801 agagcgaggt cgtcaagctg ctgccggcgc tcaccatcac gcccgacgag ctggacgagg
     4861 gcatgaagac cctcgcccgg gccgtgcgcg agacggcctg acccgcacgg caccacccat
     4921 actcccggcg ccttcggccc gcgcggccga gggcgccggg gacccttgca cacccttcac
     4981 ctaggaggca tcgcaacacc gtgatcgtcc gttcgttcaa ggagttcgaa ggcaccgacc
     5041 ggcacgtgaa gagcgcgtcc ggcacatggg agagcacgcg gatcgtcctc gccaaggaga
     5101 aggtcggctt ctccgtccac gagacgatcc tgtacgcggg tacggagacg tcgatgtggt
     5161 acgcgaacca catcgaggcc gtcgtctgca ccaagggcga cgccgagttg accgaccgcg
     5221 agacggggaa gacttaccac atcacgccgg gcacgatgta cctcctcaac ggccatgagc
     5281 ggcacacgct caaggtcaag gaggacttcc actgcatctg tgtcttcaac ccgcccgtca
     5341 ccggacggga ggatcacgac gagaacggcg tctacccgct gctcaccgag gaggtgtgag
     5401 tcaccgtgac caccacgacc acgaacgtca ctgatctcta tcccacccgc ggcgccaccg
     5461 aggtggccac ccctcggcag gacccggtcg tctggggctc cccggacacg cccggtcccg
     5521 tctcggcggg tgacctgcag tccctggacc gcgacggctt cctcgccatc gaccagctca
     5581 tcggtccgga cgaggtcggc gtctaccagc gcgagctgga gcgcctggtc ggcgacccgg
     5641 ccatccgcgc ggacgagcgc tcgatcgtcg agccgcagtc caaggagatc cgttcggtct
     5701 tcgaggtgca caagatcagc gaggtgttcg cgaagcttgt gcgcgacgag cgcgtggtcg
     5761 ggcgggcccg gcagatcctc ggctcggacg tctacgtcca ccagtcgcgg atcaacgtca
     5821 agccgggatt cggtgccagc ggcttctact ggcactcgga cttcgagacc tggcacgccg
     5881 aggacggcct gccgaacatg cggacgatct cggtctcgat cgcgctcacc gagaactacg
     5941 acaccaatgg cggtctgatg atcatgccgg ggtcgcacaa gacgttcctc gggtgcgcgg
     6001 gggccacgcc gaaggacaac tacaagaagt ccctgcagat gcaggacgcg gggacaccgt
     6061 ccgacgaggg cctgacgaag ctggcctcgg agtacggcat caagctgttc accggcaagg
     6121 ccggctcggc gacgtggttc gactgcaact gcatgcacgg gtcgggcgac aacatcacgc
     6181 cgttcccgcg cagcaacgtg ttcatcgtgt tcaacagcgt ggagaacacg gccgtggagc
     6241 cgttcgcggc accgatccgg cggccggact tcgtcggcgc gcgggacttc acgccggtgc
     6301 ggtgatccgc atctgatctc gggcaggggc cttctcgtgt gggacgggag gcccctgccc
     6361 ggtgctgtcc ccggatcctt ctctcagccc gccagcacgt cgagcagccg gtccacgtcg
     6421 gccgcggtgt tgtacaggtg gaaggcggcg cgcagattgc ccgcccggtc ggacacctgg
     6481 acgcccgccc cggccagctc gccctgccgg tggccgagcc ccggcacgga cacgatcgcg
     6541 gagcccggcg cgggcaccgg ctcgtggccg agcgagcgcg ctcccgcgcg gaaccggtcg
     6601 gcgagcgcgg tgtcgtgggc gtgcaccgcg tccacgccca gttcctcgat cagcgccagg
     6661 gagtgccggg cgcccgtgta cgagaacagg gcggggctct cgtcgaaccg ccgggcggag
     6721 cgcgcgagtt ccccgacggg gccgtagcag ctgtcccagg ggtgctcacc ggtcgcccac
     6781 cctgcgaaca ggggcgtcag cccggccgcc gctcccaggt cctgcgggac gacgaggaag
     6841 gcgacgccgc gcgggcagag cacccacttg tagccgacgg cggtcacgta gtcgaagtcg
     6901 tccgcggccg tcggcagcca gccgttcgcc tgggagacgt cgatcagcgt ccgcgccccg
     6961 tgcgcccggg cggcgtcccg cagtgcggcg aggtcggcga cccgcccgtc ggcggactgc
     7021 acggagctga ccgccacgag cgcggtgtcg gggcccaccg actcggcgag cagttccagc
     7081 ggcacgctgc gcaccttcag atcgccccgc gtgtacagcg ggttcaccag ggagctgaag
     7141 tcgccctccg cggtgaggac ttcggcgccc ggaggcagcg acgcggcgac gagcccgctg
     7201 aacgtggcga cggacgcgcc ggtcgcgacc cgggtggcgg gcactcccac gagacgggcg
     7261 tacgcggccc gggccgcctc gacgtcggcg aacatgtcga gcggctgccc gtccgccgca
     7321 aaggccgccg cgtcacgcat cgcgacgagg gtccgggcgg ggagcagtcc ggtgctggcg
     7381 gtgttcaggt acgtggtctt cggggcgaac tcggtacgga cgaggttctc gaagcttccc
     7441 atggcaccac tgtgcggccc ccggacctcc ccgtccatcg cgtgtttcta cccggtttct
     7501 ccaagcaacg cttatgtacg gccgctcacc tggggtttcg cgtgacgctt cacttctgtg
     7561 gcaccgcgca cccgtccggg ccgcaggcct ccgcgccgcc gtcgtcgatc agcttgagcg
     7621 gtgtgcgctc gccccacgcc tgggtcagcg cctgggagaa gacctcggcg ggctgggcgc
     7681 ccgagacgcc gtaggcccgg tcgagcacga agaacggcac tccggtggcc ccgagctggg
     7741 cggcctcgcg ctcgtcggcg cgcacctcgt cggcgtaggc gccggggtcg gcgagcaccg
     7801 cgcggacctc ctcggcgtcg agtccggcgc cgacggccag ttcgaggagt cgcccgtcgt
     7861 cgttgaacac cgagcgctcc tcggcgaagt tgccccggta gaacgcgtcg agcagggcct
     7921 cgtgccgccc gcgcgccttg gcgaggtgca gcaggcggtg catgtcgaag gtgctgccgt
     7981 ggtcgcggtc ccgggtgcgg tagtcgaggc cctcggcggc ggcctgggcg ccgaggttgt
     8041 cctcgccggc ccgcgcctgc gcctcgctca tgccgtactt ggcggtgagc atcgtcagca
     8101 ccggctgcac gtcgtccttg gcccgcccgg ggtcgagttc gaacgaccgg tggaccacct
     8161 cgaccccgtc acggtgcggg aaggccgcga gcgccttctc gaagcgggcc ttgcccacgt
     8221 agcaccacgg gcaggcgatg tcgctccaga tctcgacgcg catgtctcgg ctcttctcca
     8281 gctcgtacgg tggcggagac cctctccgcc ccctacgtga accttcaacc agccgcgctc
     8341 attcccgccc cgccctcagc tgccgtcccg cagatccgcg ggccagcccg gccggaactc
     8401 caggtggtcg tacgtcaccg tgcagccctc ccccgtcggc gcctgggcca ggaagccgac
     8461 cagggccgct ccggtctccg cctcgtcgcc cagtgtgaag agccgtacga aggtccagcg
     8521 ctccccgtcg agggaggcgt ggaaggcgaa ggcccgcccg ctccggctga cccggaacca
     8581 gacggagtcc ccctcgacgg tgaaggagtt gcagtcgtcg gagtgccccc gggtgaccac
     8641 cgtgcagacg gtgggcgcgt ccggggagta ctccaggcac agcttggccc aggcccgctc
     8701 ccccacgtgc acgtagagca ccccggcgtc gaaggcaccg cggaacccga cggacacccg
     8761 ggcgatcagc tggaagtcgc cgtccggcgc ccccagcagc cgcggcgcgt cggacacgga
     8821 ctccagtgcc tcccccgtgg gcggcacgaa ccggtcctgc cggggcccgg cgactccggt
     8881 gagcacaccg tcggcgtagg accaacggcc gtcgggcccg taggggcgga gagggaacgg
     8941 cagttcggga agttcaagag tcatgcactg atcatcgcag ggccggggca cgtacctgag
     9001 gggcgcgggg ctgtatcggt ttgcggctcc gcctcgtggg cgcgacaagc cacgacggcg
     9061 cgtgacccgc cgaacgaccc gcacccctac gacgcgtccg cggagcgcta ccgctcaagc
     9121 accccgttga accgccgcgg cagccccagc ggattgtcgt cccgaagctc cgcaggaagc
     9181 agcgcctcag gcgtcccctg ataagcgaca ggccgcagcc accgctcgac agccgtccct
     9241 cccaccgacg tggacgtcga agtggtggcc gggtagggcc ccccgtgctg ctgggccgcg
     9301 gccaccgcaa ccccggtcgg ccagccgttg accagcaccc gccccgccaa cggcacaagc
     9361 tccgcgagga tggacgcccc gcgtccctga cccgccgcct cctcggcgga gagctgcacg
     9421 gtagccgtca gatttcccgg cagccgcgac agcacccccc gcacctcccc ctcgtcctgg
     9481 tagcgggcca ccacggtgac cggcccgaag cactcctcca ggagcaggtc gtgttcgccc
     9541 tcctccgcca gccgcccggc cggcacggtg aggaacccgg cgctgacggt gtgctcgccg
     9601 cccgcgcccg gggtcaccgg ggagtccacg tcgggcagcg cggcgcgctc ggcgacgccc
     9661 gcgacgaagt tgtcccgcat gcggtggtcg aggagcacac cggcgccggt ggcgctgacc
     9721 gcgtcggtga gggacttgac caggccgtca ccgacgtcac cggccggcac gaggaccagg
     9781 ccgggcttca cgcagaactg gccgacgccc agcgtcatcg aaccggccag ccccgcgccg
     9841 atcgcctccg cccgttcggt gaccgccgcc tccgtgacga cgacggggtt cagcgagccc
     9901 agctcgccgt ggaaggggat cggcaccgga cgcgccgccg ccgcgtcgaa cagcgcgcgc
     9961 ccgccgcgga cggagccggt gaagccggcc gcggccacca gcgggtgctt gatcagttcg
    10021 acgcccgcct cgaagccgtg caccagaccg aggacgccgt ccgggatgtc gtggcgggcc
    10081 gcggcccggc gcagcacctt ggcgaccagc tcggacaggg ccgggtggtc ggggtgggcc
    10141 ttgacgacga ccgggcagcc ggccgccagc gcgctggcgg tgtcgccgcc ggggacggag
    10201 aaggcgaagg ggaagttgga ggcggagtag acggcgacga cgccgagcgg caccttgtag
    10261 cggcgcaggt cggggatggg cggggtggcg gtgtcgtcgg ggtggttgac gaccacgccg
    10321 aggaactcgc cctcgtcgac gatgtcggcg aaggcccgca actggtagca ggtgcgggcg
    10381 agttcgccgg tgagccgga
//
