LOCUS       NZ_CP116257            11920 bp    DNA     linear   CON 15-DEC-2024
DEFINITION  Streptomyces lienomycini strain DSM 41475 chromosome, complete
            genome.
ACCESSION   NZ_CP116257
VERSION     NZ_CP116257.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces lienomycini
  ORGANISM  Streptomyces lienomycini
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7863969)
  AUTHORS   Ding,W.
  TITLE     Streptomyces riduolalong sp. nov., isolated from the soil of Tibet
            exhibits against Gram-positive bacteria activity
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7863969)
  AUTHORS   Ding,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) School of life sciences and biotechnology,
            Shanghai Jiaotong University, 800 Dongchuan, Shanghai, Shanghai
            200240, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: GS De Novo Assembler v. August-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_027947595.1-RS_2024_12_13
            Annotation Date                   :: 12/13/2024 16:03:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,183
            CDSs (total)                      :: 7,093
            Genes (coding)                    :: 6,850
            CDSs (with protein)               :: 6,850
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 243
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 243
            Pseudo Genes (frameshifted)       :: 141 of 243
            Pseudo Genes (incomplete)         :: 121 of 243
            Pseudo Genes (internal stop)      :: 14 of 243
            Pseudo Genes (multiple problems)  :: 26 of 243
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:34:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4023060
            Orig. end                         :: 4034980
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116257.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11920
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="19"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11920
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11920
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6920](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6920
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(157..1011)
                     /locus_tag="BJ961_RS18220"
     CDS             complement(157..1011)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1097: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004993653.1"
                     /locus_tag="BJ961_RS18220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="IS5 family transposase"
                     /protein_id="WP_271416969.1"
                     /transl_table=11
                     /translation="MERTPYATDLSDEQWALIEPLVTAWKQERVARSATGDPGSCALRE
                     VVNALLYQNRTGCQWRLLPHDFPAWSAVFYYFTLWRQDGLDQRIQEILRCQVRERSRRL
                     EDPSLVIIDTQSVRVAAGVPKETTGLDANKKTPGRKRGLAVDVLGLVIGVVILAASAHD
                     NEAGIALLDQAAERCGMRLEKVLVDQGFKDAVIIHGAVKDITVEVVRRNPDDEGKGFVP
                     QPKRWVVEQVNGTLMLQRRLARDYDHRPDNAASRVYWASTAGMLRRLTTPTPTWRDDVE
                     LAA"
     misc_feature    complement(700..702)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            1725..2951
                     /locus_tag="BJ961_RS18225"
     CDS             1725..2951
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569708.1"
                     /locus_tag="BJ961_RS18225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_271413867.1"
                     /transl_table=11
                     /translation="MLRGRSGASGSGRDQSARDFRLFWCANAVDALGTQASGAVLPLLL
                     LGLGHSPRTAGLVAGLSLAAGLLLGPLAAVPADRGARKTIMFWSAVVAALAMGSVAVPL
                     LAGHLTLPHLVAAVLVERFATACHDAASRGTVALICPPDDQPRAVARLAAADQGSLILG
                     PALGGAAYQLFRALPFAADALSYAVVAGCVRAMRTDLKAAAAPSGEGLWRAAAAGLSLV
                     RRRPLLRLVLTWVALVNGVAATLYYSAVFVLQRSGSGALPLGLVLAVTGAAGLAGSLAA
                     PKAAARLGAARTLITVTWLLAPLTAALALAEGPWAYGVLLGAIGLLVPLPAVVLQARAL
                     LTTEPDLQARVGAVIATGAGATAALAPVLAGLFAGRLNPATPALGCAALLVLLAAHTTR
                     RAALAEAAA"
     gene            2948..4768
                     /locus_tag="BJ961_RS18230"
     CDS             2948..4768
                     /EC_number="3.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088741.1"
                     /locus_tag="BJ961_RS18230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="S9 family peptidase"
                     /protein_id="WP_271413868.1"
                     /sec_met_domain="PF00561 (E-value: 2.4e-05, bitscore: 22.8,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_S9 (E-value: 8.7e-36, bitscore:
                     122.1, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRFQVYRAALPEVCATDPHRMVFTADAEGRCEVFTWDAAARHAR
                     RVTDRPGGTLHGAIDADAYVWWFAEDARGVGHWQFQPFDGPFTGCPGRPGLTGVPDGTA
                     RGLAVTADATVALGIGLGDHTTVMHGTRGGPGREVARVNGPAALAGLAPHGGLLALTRT
                     ADAPGAVTLLTPDGTEVAALPGDGPHGRLWALGFGPALDDHPTLLLVRERGDRYTLATW
                     TAATGTVTHDWCALETQFTARWYPDGGAVLIRQDRHGRSLLRRADLARRTLGPVQTPPG
                     TLLDAVPRPGGDLHTLWTDSTHPPRMRSTAGTPLPPLGTVDGRVPGTLRDIHTPGPDGP
                     VHTLFSFPESTPGPHPAVFLVHGGPADHDRDAYDGTVHSLVASGFAVARVNYRGSTGYG
                     PGWQHAFRDGVGHTQVTDLAAVRADLVARGLIRPGATGLWGTSWGGYLVLLALGTRPGL
                     WQAGVAVKPVADYAAAHAAATPALRALDERLFGGTPAQVPHAYAHSSPIRYAARVRDPL
                     LIVAATNDTKCPPAQIHSYLAALDTAGTPHESLWLESGHDGYDGGDHVTVLRRAVRFLD
                     RTLRRTARTTPPPRRPAPAGEGSAHHTVEH"
     gene            4786..4914
                     /locus_tag="BJ961_RS18235"
     CDS             4786..4914
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003958124.1"
                     /locus_tag="BJ961_RS18235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_265592416.1"
                     /transl_table=11
                     /translation="MQKDVIHNDPLAEDETNRKPSVGITITVPFRNAEDGDDTVED"
     misc_feature    4944..4958
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 26.9"
                     /tool="antismash"
     gene            5001..6920
                     /locus_tag="BJ961_RS18240"
     CDS             5001..6920
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003958123.1"
                     /locus_tag="BJ961_RS18240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_271413869.1"
                     /sec_met_domain="TIGR03975 (E-value: 2.3e-185, bitscore:
                     616.2, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLLVNMPWSPIDLPSLALGILKRSVDERVPGASAEVLHANLMF
                     TDWITERTEFTGDDYEYYALSSYFMGCGDWVFSSALYDDPAWREDEFVATMSGKLKSER
                     MRMTRELHRIASDFVEMIARQIVASEPDVVGFTSTFQQNTAALAAARRVKQLAPHIVTV
                     MGGANCDGEQGATVHRNFPFVDHVVRGEGEAAFPELLGALAEGGPLDSIPGLCHRDADG
                     VHVANPMATRPLPPTAILPPDYSGYFERLASSVARNWVEPKLVVEGARGCWWGEKHHCT
                     FCGLNGSFMEFRSKSPDTFYEEIMELARRHRVLDMYVVDNILDMRYLTTVLPRIIESGY
                     DLRLHIEIKANMRRSQLRTLADAGLIYVQPGIESLNSRVLDLMDKGVSGCQNVRMLRDG
                     AETGLSVAWNYLHGFPGESAEDYDPVIAQIPALEHLDPPVDLSARIAIERFSPYFERPE
                     LGFIGLLPEAHYRFTYDLPEEELYGMAYVFEAPARGIGEPTVSALNKALADWKKHHTDA
                     RLTHDDHGDRIVLVSRRRAFDWRVKELTDPFELALFRLLDQPHNVSALTRKAAAHVPEA
                     TRDEDGVRRLLDTWLALGIVFADAGQYVHIAPAAVNEDLLRLDFMRHIHTEDAPDGAAV
                     PEPA"
     gene            6950..7606
                     /locus_tag="BJ961_RS18245"
     CDS             6950..7606
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088744.1"
                     /locus_tag="BJ961_RS18245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF5825 family protein"
                     /protein_id="WP_271413870.1"
                     /transl_table=11
                     /translation="MTATLAAPTTVLAWRDYDPVACELPGMFLGEVPLPGPARGQAARL
                     WELGARRVRLPAPVDLSTGADPAAAVYGLSLVRDLTARAVMVEWKLRLDPRDGETWRRL
                     SHLQPPAALHGPADAGESLRSWQRGHYLCKCLWRKGPGFLQIRDRRWGELSRFTADEPE
                     YDTAVRLLDYGALADTVDQAALADFRSEHLVLDVGPYAWWLPYRVSRWLQQSIAI"
     gene            complement(7676..8161)
                     /locus_tag="BJ961_RS36000"
                     /pseudo=""
     CDS             complement(7676..8161)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF018180.5"
                     /locus_tag="BJ961_RS36000"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="epoxide hydrolase family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTDPSPEIAVPDAELEDLRARLRATRWPTGWPVDGWEAGADTTEV
                     RRLAE"
     gene            complement(8272..8931)
                     /locus_tag="BJ961_RS18260"
     CDS             complement(8272..8931)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011027476.1"
                     /locus_tag="BJ961_RS18260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="WP_271417083.1"
                     /transl_table=11
                     /translation="MGFIGSGSIGSTITRLAVAAGHQVVLSNSRGPGSLADTVAELGPQ
                     ASAATSEEAAAAGDIVVVTVPVTAFPALPAAPLAGKTVIDTCNYGPERDGHIPELEGTS
                     LTSSELLLRYVPDAMLVKAFNNIYFKHLLSLARPAGAADRSYLPIAGDSGPAKAAAAEF
                     IDSIGYGVVDAGLLADSWRQATGTPVWGTPYGPPSNEKGRPTGPDAIRAALATATR"
     gene            complement(8940..9899)
                     /locus_tag="BJ961_RS18265"
     CDS             complement(8940..9899)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003977894.1"
                     /locus_tag="BJ961_RS18265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_271413872.1"
                     /transl_table=11
                     /translation="MHVFVTGGTGHSGSHIIPELIAAGHEVTGLARSDTAAATLSALGA
                     KVRRGDLRDLDGLKEAAADSDGVIHVAHRQDLLPSGGIDAVAAAELPIVLAYGEALAGT
                     GKPLVAAGSIGSPGTGRNLGRPSTEEDPALPVGDEHKGTLRARNVVETAVIGLAERGVR
                     SSVVRIANIAHSTADRAGFLPTLIALAKEKGFAGYPGDGTNLWNAVHIRDAASLFRLAL
                     EKGPAGRYWHAVEDGGIPVREIAEAIGNRLSLPAVSVPLDELMLPGHFGFLTNIVTQSY
                     PTSNLITRKTLGWEPAQPGLLADLDNGHYFPSSDVPAI"
     misc_feature    complement(9883..9898)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.09"
                     /tool="antismash"
     gene            complement(9969..10592)
                     /locus_tag="BJ961_RS18270"
     CDS             complement(9969..10592)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003977893.1"
                     /locus_tag="BJ961_RS18270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="WP_271413873.1"
                     /transl_table=11
                     /translation="MSNISFIGLGGMAHAIAARAVSGGHSVELIGRDPAKARGLASELG
                     AGATAGTFGTVPAGDIVVLAVPYTSAVPVVAQYGDALAGKVVIDISNTFNADATGLVTP
                     DGTSGAQEIAKAAPSSAHVVKAFNTVFGHVLVQERPLDVLYAGDDAGAKANVSAFIESL
                     GLRPLDVGGLEMARWLEGIGPLLMGLARNGAGTFDIALNVDLPG"
     misc_feature    complement(10687..10702)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.85"
                     /tool="antismash"
     gene            complement(<10724..10822)
                     /locus_tag="BJ961_RS18275"
                     /pseudo=""
     CDS             complement(<10724..10822)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335581.1"
                     /locus_tag="BJ961_RS18275"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="dehydrogenase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MGKLDAKVVVITGGSTGLALAGAKLFVAEGAHV"
ORIGIN
        1 cgtcgatgac cttgccggtg gcagcgagct cggccaggcg ggcttcggtc tcggcgaggt
       61 cggtggtgag ccgctcgatc tgctcgcgca ggtggtcggc gcgggcagcg gtctcatcgt
      121 gctcggtctg cagcagccgc aggagttcgc agacgttcac gcggccagct ccacgtcgtc
      181 ccgccaggtg ggggtagggg tggtgaggcg gcggagcatg ccggcggtgg aggcccagta
      241 gacgcgggag gccgcgttgt cgggccggtg gtcgtagtcg cgggcgaggc gccgctgcag
      301 catgagggtg ccgttgacct gctcgaccac ccaccgcttc ggttgcggga cgaagccttt
      361 cccctcgtcg tcggggttgc ggcggaccac ctcaacggtg atgtccttca ccgccccgtg
      421 gatgatcacg gcgtccttga agccctggtc gaccaggacc ttctccaggc gcatcccaca
      481 ccgctcagcc gcctggtcca gcagggcgat gccggcttcg ttgtcgtgcg cgctcgcagc
      541 gaggatcacc acaccgatga ccaggcccag cacatcgacg gccagtcccc gcttcctgcc
      601 gggcgtcttc ttgttcgcgt ccagtcccgt cgtctccttc ggcaccccgg ccgccacgcg
      661 gacggactgg gtgtcgatga tcaccaggga cgggtcctct aatcgtctgg accgttcccg
      721 cacctggcag cgcaggatct cctggatccg ctggtcaagg ccgtcctggc gccacagggt
      781 gaagtagtag aacactgccg accaggccgg gaagtcgtgc ggcagaagcc gccactgaca
      841 gcccgtccgg ttctggtaaa gcagcgcgtt cacgacctcg cgcagggcgc aggagcccgg
      901 gtctccggtc gccgaccgcg ccacccgctc ctgcttccac gcggtgacca gcggctcgat
      961 caacgcccac tgctcgtcgg acaagtcggt tgcgtacggc gtccgttcca tgcccacgac
     1021 cccatcatgt acacgccatg acacgaggac ggaactgtcc catcacccac gaacgggcga
     1081 caccacatca tcaggaacgg gacttaacgc cctctgaaat ctctcgatct tcccggtggt
     1141 ggtcggagag cggggtgtgg tcagccgggc ggcctctcag caagccaggt caggtatcac
     1201 ccatccgtgc agcagtcccg gtcgttgagg ctcgtgccgt ggcgaagtga gtcatcagcg
     1261 atgcctgccg cgctccgcag gggcgcggca ctacgcatgc catggcggca cccggcggcg
     1321 tgaactggcc atcctcggcc gggaaagcac cggcaccggc ccggcaccgg ccccgtccga
     1381 cgtcttcccg gaccgcttcg gcaggctcgg cgcttgacca taggcatgcc aacgacacgc
     1441 ctcacacgtc acacgcgtct ctcacacctc gccctcaccg cggaccgcac cggaacacac
     1501 gcctcccctt actgcgaacc cggaatcccc accccctcag cgccccctcg cgctttccag
     1561 ccaaccgatc cgcgaccttg tagcccgttc gaggcgctgg ctacgatccg cctcgtttcg
     1621 ctttctacac aacctgcacg atcgcttcat ccaacggggg aatcgtggag tcgttcacac
     1681 aggcgggagc ggcggacccc cgcggtggat taccccggcc ccggttgctc cggggtcgca
     1741 gtggcgcttc cggttccggg cgcgaccagt ccgcccggga cttccgtctc ttctggtgcg
     1801 ccaacgccgt cgacgcacta ggcacccagg catccggcgc cgtccttccc ctgctgctcc
     1861 tcggtctcgg gcactcaccg cggaccgctg ggctcgtcgc cgggctctcg ctggccgcgg
     1921 gtctgctgct cgggccgctc gcggccgtcc ccgccgaccg cggcgcgcgc aagacgatca
     1981 tgttctggtc cgccgtcgtc gccgcgctcg ccatgggctc cgtcgccgtg cccctgctcg
     2041 ccggacacct gacgcttccg cacctcgtgg ccgccgtcct cgtcgaacgg ttcgccaccg
     2101 cctgtcacga cgccgcgtcg cgcggcacgg tcgccctgat ctgcccgccc gacgaccagc
     2161 cgcgcgccgt ggcccggctc gccgccgccg accagggctc actcattctc ggcccggccc
     2221 tcggcggagc cgcgtaccag ctctttcgcg ctctgccgtt cgcggccgac gccctctcct
     2281 acgccgtcgt cgccggctgc gtacgggcca tgcgtacgga tctgaaggcc gcggcggctc
     2341 cttcgggcga ggggttgtgg cgtgcggccg ccgccggcct gtccctggtg cgacgacggc
     2401 cgttgctgcg cctcgtgctg acgtgggtcg cgctggtcaa cggggtggcc gcgacgctct
     2461 actactccgc ggtcttcgtg ctgcaacgtt ccggcagtgg tgcgctcccg ctcggcctcg
     2521 tcctcgccgt caccggcgcc gcggggctgg ccgggtccct cgcggcgccc aaggccgccg
     2581 cccgtctcgg cgccgcccgc accctcatca ccgtcacctg gctcctcgcc ccgctcaccg
     2641 cggccctcgc cctcgccgag gggccctggg cgtacggcgt actgctcggc gcgatcggcc
     2701 tcctcgtgcc gctgcccgcc gtcgtcctcc aggcccgtgc cctgctgacg accgagcccg
     2761 acctgcaggc ccgcgtcggc gccgtcatcg ccaccggcgc cggcgccacc gccgccctcg
     2821 ccccggtcct ggccggtctc ttcgccggcc ggctgaaccc ggcgacgccc gcgcttggtt
     2881 gcgccgccct cctcgtcctg ctcgccgcgc acaccacccg gcgcgctgcc ctcgcggagg
     2941 ccgccgcatg agccgcttcc aggtgtaccg cgccgcgctg cccgaggtgt gcgccaccga
     3001 tccgcaccgc atggtgttca ccgccgacgc agagggccgc tgcgaggtct tcacctggga
     3061 tgccgccgcc cgccacgccc gccgcgtcac cgaccgcccc ggcggcaccc tgcacggcgc
     3121 gatcgacgcc gacgcatacg tgtggtggtt cgccgaggac gcgcggggcg tcggccactg
     3181 gcaatttcag ccgttcgacg gcccgttcac cggctgcccc ggccggcccg gtctgaccgg
     3241 cgtccccgac ggcaccgccc gcggcctcgc cgtcaccgcc gacgccaccg tcgccctcgg
     3301 catcggactg ggcgaccaca ccaccgtcat gcacgggaca cgcggcggcc ccggacgcga
     3361 ggtcgctcgt gtgaacggcc ccgccgcgct cgccggactc gccccgcacg gcggcctcct
     3421 cgctctcacc cgcaccgccg acgcgcccgg cgccgtcacc ctcctcaccc ccgacggcac
     3481 cgaggtcgcg gccctccccg gtgacggccc gcacggcaga ctgtgggcgc tcggcttcgg
     3541 ccccgcgctc gacgaccacc ccaccctgct gctcgtccgc gagcgcgggg accgctacac
     3601 cctcgccacc tggaccgccg ccaccggcac cgtcacgcac gactggtgcg ccctggaaac
     3661 ccagttcacc gcccgctggt atccggacgg cggcgccgtg ctgatccggc aggaccggca
     3721 cggccgctcc ctgctccgcc gcgccgacct cgcccgccgt actctgggcc ccgtacagac
     3781 tccgcccgga acgctcctgg acgcggtccc gcgccccggc ggcgacctgc acaccctctg
     3841 gacggacagc actcacccgc cccgcatgag gtccacggcc gggactccgc tgcccccgct
     3901 cggcaccgtc gacggccgtg tgcccggcac tctgcgcgac atacacacac cgggaccgga
     3961 cggcccggta cacacgttgt tcagctttcc cgagtccaca cccgggccgc accccgccgt
     4021 gttcctcgtc cacggcggcc cggccgacca cgaccgcgac gcctacgacg gcaccgtgca
     4081 ctccttggtg gcctccgggt tcgccgtggc ccgcgtcaac taccgcggct ccactggata
     4141 cggaccgggc tggcagcacg ccttccgcga cggcgtcggc cacacccagg tcaccgacct
     4201 cgccgccgtc cgcgccgacc tcgtggcgcg cgggctcatc cggccgggcg ccaccggact
     4261 ctggggcacc tcctggggcg gttacctcgt cctgctcgcc ctcggcaccc gcccggggct
     4321 ctggcaggcg ggcgtcgccg tcaaacccgt tgccgactac gccgccgcac acgccgccgc
     4381 cacacccgcc ctccgcgcgc tggacgagcg gctgttcggc ggcacacccg cacaggtgcc
     4441 gcacgcatac gcgcacagct cgccgatccg ctacgccgca cgcgtccgcg atccgctcct
     4501 gatcgtcgcc gccacgaacg acaccaagtg cccgcccgca cagatccatt cctacctcgc
     4561 cgccctcgac acggccggta ccccacacga gtccctgtgg ctcgagtccg ggcacgacgg
     4621 ctacgacggc ggcgaccacg tgaccgtcct gcgccgcgcg gtgcgcttcc tcgaccgcac
     4681 gctccggcgc accgcccgca ccaccccacc ccccagacgc cccgctccgg ccggagaggg
     4741 gtcggcgcac cacacagtcg aacactgaga gagaaggaga aacccatgca gaaggacgtc
     4801 atccacaacg acccgctggc cgaggacgag acgaaccgca agccgagcgt cggcatcacg
     4861 atcaccgtcc cgttccgcaa cgcggaggac ggcgacgaca ccgtcgagga ctgatcctca
     4921 ccggtcggcc ccagggcgcc ccggggccga ccggcccgca ccgtcgcccg cccccgcacc
     4981 acgtcaggag agaagcgccc atgcgcgtac tgctggtcaa catgccctgg tcgcccatcg
     5041 acctgccgtc cctagccctc ggcatcctca aacggagtgt ggacgagcgc gtgcccggtg
     5101 ccagtgccga ggtgctgcac gccaacctca tgttcaccga ctggatcacc gaacgcaccg
     5161 agttcaccgg cgacgactac gagtactacg ccctctcctc ctacttcatg ggctgcggcg
     5221 actgggtctt ctcctccgcc ctgtacgacg acccggcctg gcgcgaggac gagttcgtgg
     5281 cgacgatgag cggcaagctc aagtccgagc ggatgagaat gacccgcgaa ctgcaccgca
     5341 tcgcatcgga cttcgtcgaa atgatcgccc ggcagatagt ggcgagcgag ccggatgtcg
     5401 tcgggttcac ctccactttc cagcagaaca cagccgcgct cgccgccgcc cgccgcgtca
     5461 agcagctcgc cccgcacatc gtgaccgtca tgggcggcgc caactgcgac ggcgaacagg
     5521 gcgccaccgt gcaccgcaac ttccccttcg tcgaccacgt cgtacgaggc gagggcgagg
     5581 cagccttccc cgaactcctc ggcgcgctcg cggagggcgg cccgctcgac tcgattcccg
     5641 gcctgtgcca ccgcgacgcg gacggcgtgc atgtcgccaa cccgatggct acccgcccgc
     5701 tgccgcccac cgcgatcctg ccgcccgact acagcggcta cttcgaaagg ctcgcctcgt
     5761 ccgtcgcccg caactgggtc gagccaaaac tcgtcgtcga gggggcgcgg ggctgctggt
     5821 ggggcgagaa acaccactgc accttctgcg ggctcaacgg ctccttcatg gagttccgct
     5881 ccaagagccc cgacaccttc tacgaggaga ttatggagct ggcccgtcgc caccgggtcc
     5941 tcgacatgta tgtcgtcgac aacatcctcg acatgcgcta cctcaccacc gtgctgcccc
     6001 gcatcatcga gagcggctac gacctgcgcc tgcacatcga gatcaaggcc aacatgaggc
     6061 gcagtcagct gcgcacactc gccgacgcag gcctcatcta cgtgcaaccc ggcatcgaga
     6121 gcctcaacag ccgcgtgctg gacctgatgg acaagggtgt gagcggctgc cagaacgtgc
     6181 ggatgctgcg cgacggcgcc gagaccgggc tctccgtcgc ctggaactac ctgcacggat
     6241 tccccgggga gagtgctgag gactacgacc cggtcatcgc ccagataccg gccctggaac
     6301 acctggaccc accggtcgac ctgtccgcgc gcatcgcgat cgagcgtttc agcccctact
     6361 tcgaacgccc cgaactgggc ttcatcggtc tgctccccga agcgcactac cgcttcacct
     6421 acgacctgcc cgaggaagag ctgtacggca tggcgtacgt cttcgaggcg cccgcccgcg
     6481 gcatcggcga acccaccgtc agcgccctca acaaggccct cgccgactgg aagaagcacc
     6541 acaccgacgc ccggctcacc cacgacgacc acggcgatcg catcgtcctg gtcagccgtc
     6601 gccgcgcttt cgactggcgg gtgaaggagc tcaccgaccc cttcgaactc gccctcttcc
     6661 gcctcctcga ccagccgcac aacgtgtcgg ccctcacccg caaggccgcg gcacacgtcc
     6721 ccgaggcgac ccgcgacgag gacggggtcc ggcggctcct cgacacctgg ctcgccctcg
     6781 gcatcgtctt cgccgacgcg ggacagtacg tccacatcgc cccggccgcc gtcaacgagg
     6841 acctgctgcg cctggacttc atgcgacaca tccacaccga ggacgccccc gacggtgccg
     6901 ccgtccccga gccggcctga cccctcccgc cccgcttcga aggagcacga tgaccgccac
     6961 cctcgccgcg ccgacgacgg tcctcgcctg gcgcgactac gaccccgtcg cgtgcgagct
     7021 gcctggcatg ttcctgggcg aggtgcccct gcctggcccg gcgcgcggcc aggctgcacg
     7081 cctgtgggag ctaggcgcgc gccgtgtgcg gctgcccgcc cccgtcgacc tctccaccgg
     7141 cgcggacccg gcagccgccg tgtacgggct ctccctggtt cgggacctga ccgcgcgggc
     7201 ggtcatggtc gagtggaaac tgcgtctcga cccgagggac ggggagacct ggcgacggct
     7261 cagccatctc cagccgcccg ccgcgctgca cggccccgcc gacgccgggg agtcgctgcg
     7321 ctcatggcag cgcggtcact acctgtgcaa gtgcctgtgg cgcaaaggcc ccggcttcct
     7381 gcagatccgc gaccgtcgct ggggagagct gagccgcttc accgccgacg aacccgagta
     7441 cgacaccgcg gtccgcctgc tggactacgg cgccctcgcc gacaccgtcg accaggccgc
     7501 gctggccgac ttccgctccg aacacctcgt cctagacgtc ggcccgtacg catggtggct
     7561 gccgtaccgt gtcagccggt ggctccagca gtcgatcgcc atctgaagga gcggggctcg
     7621 atcgtggccg aggaggaagc gccggccggc ggcacggtgt acccggacgc cgtgctcagc
     7681 cgcggcgacc tggtcgagcg ccctcgcagc ggcctcggga gggccgggcg ctgcggcgga
     7741 acgcgtagcc gggcagcgag ggggcgacga ccgtgaacac gtcggacgcg tcgccgccgt
     7801 gctgcggcgg ctccgcgaga cgccgggcga ggtcggtgag ctccaggaag gtgctgggcg
     7861 agccgtgggt caggacgatc ggcaggacgc cgggctgctc gccgtcgaac cggaggcaat
     7921 ggacgggcgt gccgccgatg gcggccgtgt ggtgcggcag ggcattgatc cgcgcctcgt
     7981 gggtgcgcca gtcatagccg gtggcccact actccgccag gcggcgcacc tcagttgtgt
     8041 cggcgcccgc ttcccagccg tcgacgggcc acccggtggg ccaccgggtg gcgcgtagcc
     8101 gcgcgcgcag gtcttcgagt tctgcgtcgg gcaccgcgat ctccggcgac gggtcggtca
     8161 tgtgcccctc ctgaccgtgc tcacgtgctg gtcgtgattc tgtcttcgca tcggtcgcag
     8221 tcggcgcagc atgatcgcga ccggaccata ctgcggcaca cgccgacttg attaccgcgt
     8281 tgcggtggcc agcgccgcgc ggatggcgtc cgggccggtc ggccggccct tctcgttcga
     8341 gggcggcccg tacggggttc cccacaccgg tgtgcccgtc gcctgccgcc agctgtcggc
     8401 caggagtccc gcgtccacca cgccgtaccc gatggaatcg atgaactcgg cagctgccgc
     8461 cttcgccggc ccggagtccc cggcgatcgg caggtacgag cggtcggccg cccccgccgg
     8521 gcgggcgagc gacaacaggt gcttgaagta gatgttgttg aacgctttca ccagcatggc
     8581 gtccgggacg tatcgcagca gcagctcgct cgaggtgagc gacgtgccct ccagctcggg
     8641 gatgtgcccg tcgcgctcgg ggccgtagtt gcacgtgtcg atgaccgtct tcccggccag
     8701 cggtgcggcg ggcagggcgg ggaaggccgt gaccggcacc gtgaccacga cgatgtcacc
     8761 ggccgccgcg gcctcctcgc tcgtcgccgc cgacgcttgg ggacccagtt ccgcgaccgt
     8821 gtccgcgagc gacccgggac cgcgcgagtt gctgagcacg acctggtgtc cggccgcgac
     8881 ggcgagccgc gtgatcgtgc tgccgatgga cccgcttccg atgaatccca cagtggtcat
     8941 cagattgcgg ggacgtcgga tgacgggaag tagtggccgt tgtccaggtc ggcgaggagg
     9001 ccgggctggg cgggttccca gccgagggtc ttgcgggtga tgaggttgga cgtcgggtag
     9061 ctctgcgtga cgatgttggt gaggaacccg aagtgccccg gcagcatcag ttcgtccagg
     9121 ggcacgctca ccgcgggcag gctcaggcgg ttgccgatgg cctccgcgat ctcgcggacc
     9181 gggatgcccc cgtcctcgac cgcgtgccag tagcggccgg ccgggccctt ctccagtgcc
     9241 aggcggaaca gggaagcggc gtcgcggatg tgtacggcgt tccacaggtt cgtgccgtct
     9301 ccagggtacc cggcgaagcc cttctccttc gccagggcga tcagtgtggg gaggaagccg
     9361 gcacgatcgg ccgtgctgtg cgcgatgttg gcgatccgta cgaccgaaga ccggactccc
     9421 cgctcggcga ggccgatgac cgcggtctcg acgacgttac gggcccggag ggtgcccttg
     9481 tgctcatcgc cgacggggag ggcgggatcc tcctcggtgg acggcctgcc caggttccgc
     9541 ccggtcccgg gcgagccgat gctccccgcc gcgaccagtg gctttccggt tcccgcgagt
     9601 gcctcgccgt aggcgagcac gatcgggagc tcggcggcgg ccacggcgtc gatcccgccg
     9661 gacggaagca ggtcctgcct gtgcgcgacg tggatgacgc cgtcggagtc cgcggccgcc
     9721 tccttgagcc cgtcgagatc ccggaggtcg ccgcggcgga ccttcgcgcc aagcgcggac
     9781 agggtcgccg cggccgtgtc cgaccgggcc aggccggtga cctcgtgccc tgcggcgatg
     9841 agttcgggaa tgatgtgcga accggagtgg ccggtcccgc cggtgacgaa gacgtgcacg
     9901 ttactcctcc tggtgagtga tgcggcgtga gtggtacggc gccgaagaag cggaaatgcg
     9961 gggtgcgctc agccgggcag gtcgacgttg agggcgatgt cgaaggtgcc cgcgccgtta
    10021 cgggccaggc ccatcagcag cggcccgatc ccttccagcc aacgagccat ctccaggccg
    10081 ccgacatcga gcgggcgcag tccgaggctc tcgatgaagg ccgacacgtt cgccttggcg
    10141 cccgcgtcgt ctccggcgta gagcacgtcc aacgggcgct cctgaaccag gacgtggccg
    10201 aagacggtgt tgaacgcctt cacgacatgc gcgctcgacg gggccgcctt ggcgatctcc
    10261 tgcgcaccgg atgtgccgtc gggggtgacg agcccggtgg cgtcggcgtt gaaggtgttg
    10321 gagatgtcga tgacgacctt gccggccagg gcgtctccgt actgggcgac gaccggcaca
    10381 gcgctggtgt acggcaccgc gaggacgacg atgtcgcccg cggggaccgt gccgaacgtc
    10441 cccgccgtgg caccggcgcc gagttcggat gccaggcccc tggccttggc cgggtcgcgg
    10501 ccgatgagct cgacggagtg gccgcccgag accgcgcgtg cggcgatggc gtgggccatg
    10561 cccccgagac cgatgaaact gatgttgctc atggtgagtg ctctctttcc tgatggtcgt
    10621 ttcttgggtg atcgtctctt gggaggtcgc agccggccgg tgcgctcgat cacctgggcg
    10681 gcgaagtcga gcagttggtc aaagctggtg aacatcgcga aggcacgtgc gctccttcgg
    10741 cgacgaacag cttggccccg gccagtgcca ggccggtgga cccaccggtg atcaccacta
    10801 ccttggcatc gagctttccc acggccactc ctttgaattg atgaatttcc gaaacgggaa
    10861 aggaaggggc tggtaggggc tcaattcatc atttcttgat gcgcatgtgc tcatccttgt
    10921 tttgaaccca gccgccgaac gacgatgccc aattcttgaa ccgagttgtc ccggcccaat
    10981 taacgatgtc accagcctga tcgtgtgtcc aagacctgtt tcaaccgcgg tgataccctt
    11041 aaggcattgc cggaccggga ggccccgtgg atctggacct gcgcaagctg cgctacttcg
    11101 tcgccgtcgc cgaccggttg cacttcggcc gcgccgccga tgagctgcac atcgcgcaac
    11161 cggcgctcag ccggcagatc cgcgcactgg aacaggatct cggcgcctcg ctgttcaccc
    11221 gggacagtca cggcgtcacg ctgaccgacg cgggccgaca actgctggac gacgcgggcc
    11281 cgctgctcgc ctccgcgcac gcggtccggc gccgagtctc cgtggcacaa cgcggcggcc
    11341 agcggctggt ggtcggcttc cgggcaggca tcccggtcat ccccgcgacg cgggtgttcg
    11401 aggcgcagca tccggacgtg gtcgtggacg tgcagcgcat ggaatgggac gaccagggct
    11461 cgatgctgct cgacggccgc gtcgacgtcg cctatgtgcg gctgcccatc gacgagacgg
    11521 gcctgcgcct gactccgctc tacaccgagc ccctcatggt ggcgctgccc accgatcacc
    11581 ggctggccgg caaggatcag gtcaccgagg ccgacctggc cggtgagccg ctgatctggc
    11641 atgccgacgc gagcacgcag cccacccggc gcccgcaccc ggactccgga ctccgggtgc
    11701 gcggcgtgga ggagaagctc gagcacgtcg cggccggccg cggcatctcg ttcgtgggac
    11761 gttcggagac cgtgttcttc tcacgtccgg atatcagcta cgtgcccgtc ccggacctgg
    11821 cgcccgacca ggtattcgtc gcaatggcgg catcgcgcac ctcgccgctg gccgacgact
    11881 tcttcgccgc ggctcaggcg gcatccgaga tcacggcaga
//
