LOCUS       NZ_CP116257            10216 bp    DNA     linear   CON 15-DEC-2024
DEFINITION  Streptomyces lienomycini strain DSM 41475 chromosome, complete
            genome.
ACCESSION   NZ_CP116257
VERSION     NZ_CP116257.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces lienomycini
  ORGANISM  Streptomyces lienomycini
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7863969)
  AUTHORS   Ding,W.
  TITLE     Streptomyces riduolalong sp. nov., isolated from the soil of Tibet
            exhibits against Gram-positive bacteria activity
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7863969)
  AUTHORS   Ding,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) School of life sciences and biotechnology,
            Shanghai Jiaotong University, 800 Dongchuan, Shanghai, Shanghai
            200240, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: GS De Novo Assembler v. August-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_027947595.1-RS_2024_12_13
            Annotation Date                   :: 12/13/2024 16:03:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,183
            CDSs (total)                      :: 7,093
            Genes (coding)                    :: 6,850
            CDSs (with protein)               :: 6,850
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 243
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 243
            Pseudo Genes (frameshifted)       :: 141 of 243
            Pseudo Genes (incomplete)         :: 121 of 243
            Pseudo Genes (internal stop)      :: 14 of 243
            Pseudo Genes (multiple problems)  :: 26 of 243
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:34:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3340984
            Orig. end                         :: 3351200
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116257.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="14"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5216)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(1873..4095)
                     /locus_tag="BJ961_RS15065"
     CDS             complement(1873..4095)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978106.1"
                     /locus_tag="BJ961_RS15065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_271413332.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 2e-38, bitscore:
                     129.9, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTAQETRGRRRAAPPGRGVPKGRAKTVRTPTVLQMEAVECGAAS
                     LAMVLGHYGRHVPLEELRIACGVSRDGSRASNLLKAARRYGLTAKGMQMDLAALAEVTA
                     PAILFWEFNHYVVYDGMGRRFGRRGVFVNDPGKGRRFVSLEEFDGSFTGVVLTMVPGDG
                     FTRGGRRPGVLGAMPARLRGTAGTLPAAVLASLLLVAVGAAVPALSRTYIDLFLIGGQT
                     SLLGVLFASMGACVLLTVVLTWLQQANLLHGRIISSTLSSARFLRHLLRLPVTFFSQRS
                     PADLVQRLQSNDAVAETLARDLAAAGVDAIVVVLYAVLLYTYDPQLTFVGIGVALLNIL
                     AMRVVIRLRATRTAKLRADTARLTNTAYTGLQLIETMKATGGEDGYFRKWAGQHATTLE
                     EQQRLGVPSAWLGVVAPTLATLNSGLILWIGGMRAVEGHISVGLLVAFQALVVRFTAPL
                     TRLNGVAGRIQDFAADVARLKDVENFRADPLYDRPGGADSTRRLRGHVELQNVSFGYNP
                     LDEPLLTGFDLTVGPGQQVALVGGSGSGKSTVSRLISGLYAPWDGVIRVDGQRLDDIPR
                     GALASSVSFVDQDVFLFEGSVRDNVALWDPSIPDDAVVEALRDAALYDVVTRRPGGVHS
                     RVEQDGRNFSGGQRQRLEIARALVRRPSVLVLDEVTSALDAETELVVMDNLRRRGCACV
                     VIAHRLSTVRDSDEIVVLEHGTVVERGRHEELVARGGAYAALVGER"
     gene            complement(4092..4898)
                     /locus_tag="BJ961_RS15070"
     CDS             complement(4092..4898)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="BJ961_RS15070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_271413333.1"
                     /transl_table=11
                     /translation="MQFRQQALTKLQSPEGLDLPVRFARPQGWLALSVTVVAMAAASVW
                     AVGGSVTSTVAAPAVLTHGQGSYLLQSPVAGQVTAVLARPGQRLAADAPVLKVRTSEGE
                     TVVRSVDAGRVSGLAATVGQIIATGANVASVEKVAHADDPLRATVYVPAESAAAIPAHA
                     SVDLTVQSVPTQRYGVLHGEVKSVDRSAQSAQTIGAFLGDGALGEQFTKDGRPVAVTVE
                     LAESTSTRSGYEWSSADGPPFELTSMTLAAGSIRLADRRPVDWLLP"
     gene            complement(5001..5216)
                     /locus_tag="BJ961_RS15075"
     CDS             complement(5001..5216)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128984.1"
                     /locus_tag="BJ961_RS15075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type A2 lantipeptide"
                     /protein_id="WP_271413334.1"
                     /sec_met_domain="strepbact (E-value: 5.5e-36, bitscore:
                     121.6, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSTPRVETVEISDADLDNVSGGLNVNAAGTVTGLVDGIAPVSGL
                     LNTAVGTVEGVTGLNTAPVTGLVAGL"
     gene            5797..7026
                     /locus_tag="BJ961_RS15080"
     CDS             5797..7026
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978109.1"
                     /locus_tag="BJ961_RS15080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="S1 family peptidase"
                     /protein_id="WP_271413335.1"
                     /transl_table=11
                     /translation="MSHQRIPKRRAAMAAGGVVALGAAAILLPTANASQGGGSPDDAAS
                     APRTLKAADASQLASRLAGLLGDTFAGSYYDSGAQRLVVNVAGGDDNPVVADAEKAGAD
                     VREVDHSMGELRGGARTLKSEASIPGTSWAIDPRTNRILVTADSTVTGDRWDRLESTVE
                     RLGSGMATVRKSAGTFRTFASGGDAVFGGGARCSLGFNVTAGDGSPAFLTAGHCGVAAD
                     RWSDAQGGQPTATVDRAVFPGAGDFALVTYDDPATDAPSEVNLGDRALPISRAAEATVG
                     QEVFRMGSTTGLADGQVLGLDATVNYPEGTVTGLVQTDVCAEPGDSGGSLFTGDGLAIG
                     LTSGGSGDCTVGGETFFQPVTTALDAVGATLGDGDTGAGDTGVADGTGAGHEDGSGPTG
                     RRADGLAGGR"
     misc_feature    complement(7056..7069)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: medium, score: 20.4"
                     /tool="antismash"
     misc_feature    7166..7181
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.08"
                     /tool="antismash"
     misc_feature    complement(7169..7184)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.64"
                     /tool="antismash"
     gene            7182..7361
                     /locus_tag="BJ961_RS15085"
     CDS             7182..7361
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007389406.1"
                     /locus_tag="BJ961_RS15085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_271413336.1"
                     /transl_table=11
                     /translation="MTTDTTRKPAVRRLETGWSKARRLRSRGVMRTCVPAVEDRGTARA
                     PHLGQESNIVRGED"
     gene            7527..8501
                     /gene="rfbB"
                     /locus_tag="BJ961_RS15090"
     CDS             7527..8501
                     /EC_number="4.2.1.46"
                     /GO_function="GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /GO_process="GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (4-179). E-value: 7.3e-21.
                     Score: 66.3. Matches aSDomain:
                     nrpspksdomains_BJ961_RS15090_NAD_binding_4.1"
                     /codon_start=1
                     /gene="rfbB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019327749.1"
                     /locus_tag="BJ961_RS15090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="WP_271413337.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 1.3e-17, bitscore:
                     62.5, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 5.8e-19,
                     bitscore: 67.0, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 8.6e-19, bitscore:
                     66.3, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNILVTGAAGFIGSRYVRTLLARDAPDEPRITVLDALTYAGTTAN
                     LQLDHPRLEFVHGDIRDTALVDKLAAEADQVVHFAAESHVDRSIHAAADFVLTNVVGTQ
                     NLLDAALRHGVGTFVHVSTDEVYGSVETGSATEEHPLRPSSPYSASKASGDLLALSYHR
                     THGLDVRVTRCSNNYGPHQFPEKLVPLFVTHLLDGRRVPLYGDGRNVRDWLHVDDHCRG
                     VDLVRTRGRAGEVYNIGGGTELSNLDLTGLLLDACDAGPDRIVHVADRKGHDLRYSVDW
                     SKAREELGYRPRHDFPAGLAETVAWYRDNRAWWEPLKRRTDVA"
     misc_feature    7528..7542
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.26"
                     /tool="antismash"
     aSDomain        7539..8063
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BJ961_RS15090_NAD_binding_4.1"
                     /evalue="7.30E-21"
                     /label="BJ961_RS15090_NAD_binding_4.1"
                     /locus_tag="BJ961_RS15090"
                     /protein_end="179"
                     /protein_start="4"
                     /score="66.3"
                     /tool="antismash"
                     /translation="VTGAAGFIGSRYVRTLLARDAPDEPRITVLDALTYAGTTANLQLD
                     HPRLEFVHGDIRDTALVDKLAAEADQVVHFAAESHVDRSIHAAADFVLTNVVGTQNLLD
                     AALRHGVGTFVHVSTDEVYGSVETGSATEEHPLRPSSPYSASKASGDLLALSYHRTHGL
                     DVRVTRCSNNYG"
     gene            complement(8518..9102)
                     /locus_tag="BJ961_RS15095"
     CDS             complement(8518..9102)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027: isochorismatase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978113.1"
                     /locus_tag="BJ961_RS15095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hydrolase"
                     /protein_id="WP_271413338.1"
                     /transl_table=11
                     /translation="MSVTTLDPRTALVVIDLQAGIVAAPTEPYTGPEVVARSAELAEAF
                     RARDLPVVLVRVSFAADGADAVPGRTEAGGAGGPRPEGWDVVVGELAGHPGDITVTKRN
                     WGAFHGTGLDVQLRRRGVTQIVLCGIATSIGVESTARAAHEHGYHVTLATDAMSDMSAQ
                     AHRGSVERIFPRLGETGTTREVLELLAKTHC"
     gene            9206..9706
                     /locus_tag="BJ961_RS15100"
     CDS             9206..9706
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Metallophos_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007389408.1"
                     /locus_tag="BJ961_RS15100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="metallophosphoesterase"
                     /protein_id="WP_271413339.1"
                     /sec_met_domain="Metallophos_2 (E-value: 1.9e-27, bitscore:
                     95.0, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLLIMSDTHLPKRAKELPAPLVAEIPRADVVLHAGDWVDTATLD
                     LLESRSRRLVGVHGNNDGPALRARLPEVAYADLGGLRFGVVHETGAAQGREARCAARFP
                     GLDVLVFGHSHIPWDTTAATGLRLLNPGSPTDRRRQPYRTYMTATVTDGRLGAVELHRL
                     PTG"
     gene            complement(9761..10126)
                     /locus_tag="BJ961_RS15105"
     CDS             complement(9761..10126)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007389401.1"
                     /locus_tag="BJ961_RS15105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="WP_271413340.1"
                     /transl_table=11
                     /translation="MSTTADPGFDTEALRRGIEGKTAAPLLSLYARDARVRVVDRYDQP
                     SRPKVLHGRDEIAEMLDDVYSRDMTHRMERCVVQGDQVAFTEECTCPDGVRVLAESMLS
                     LRDGEIVEQTMIQAWDE"
     misc_feature    10172..10186
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 27.18"
                     /tool="antismash"
ORIGIN
        1 ggcaggtaga ccgcgccgag gaccgcgttc aggttcttga tccggcccgc gcgccgctgc
       61 agttcacggc tgtgcgcgaa ccgctccgcc cacgccgcgt aggcgtagtt ctcggcggcc
      121 gccacccgca gcttgggcag cccgctcagt gtctggaacg cctggttgtt cagcttgttg
      181 gtgagcttga ccagccgccg ctgccagcgc acctgccaca gtccgagccc caggaacacc
      241 ccggcgacga ccaccagcac gccgatcgcc gcgagcgcca tcggcacgct gtaccagagc
      301 agcagcccca ggttcatcac cccgaccgtc accgactgga cgaccgtcgg gccgaccccc
      361 gcgaggagtc tgcggatcgc gctgacgccc atggccgcgc tcgccagctc gccggtggaa
      421 cgctcggtga agaaccccgt cggcagccgc agcagccggt cccacacggc aggctggagc
      481 gtcgcctcga tccggccctc caggcgcagg atggtgaggt tctgcaccag catgaaggcc
      541 gccgcgacga cgctgctcag catcaccgcg aggcacacct gcgcgatcag cccggtctgc
      601 gccgtcggca cgaactcgcc gagcacccgg cccgtggcga tcggcaccag cgctccgagc
      661 gccaccgtca ccagaccggc gagcagcagg gacgtcaggt cccggcgcgt gcccgccagg
      721 cagaaccgca gcagccgcag cggaccgagc cggcgatccg gcaacggccg gtagaacatc
      781 accgcgcgcg gctcgaactc cccggcgttc gccttctcca ccggcgtctc ccgaccggtc
      841 gccggatgca cggccgcata accgccgcgc cgccacagca gcgccaccgg cgcccccgac
      901 agggtgcggt gcccgaccag cggccccacg tcgtcgcgcc accagcggtc ctccagccgc
      961 accggcctgg tccgcacgcg cgaggcgagc gcgatgcgct ccaccgggtc gagccggtcg
     1021 ctctccgcgt tgcccgtgcc cggctcggcc agcggaatcc cggcggcgcc ggcgaccagc
     1081 ctgcaggccg cgtacgtggc gtcggcgtcg gctgccgtgg tgcgccccgc cgaacgcttg
     1141 ccgagggagg ccagcagcgt ccggtcggcc tgcgcgcgca ccgcctcacc ggccttgata
     1201 ccggccgccg tccgggtctc gtgggtgcgc tccacctgct cgatccaccg gtccagggtg
     1261 gtcagcagcc ggtactgctg gtcgaccagg ctctgccaca gcgccgggtc catcagcagg
     1321 tcggaggccg cctcctcgcc gtagaccgcg ccgtaccgca cgctgcccgg cgggacctgc
     1381 atccagaaca cgtcgtcgtc cgtgggcgcc gcggcccggt cggaagccat cggcgcctgg
     1441 aacagcacgg tcagaccgcg gcccacgccg agcgcgaggg cgtactccag cggactggtc
     1501 gtcggcggca cgtaccgcgg gttgccgtac gcgtcgtacg accacgtctg cgtgtgcgcc
     1561 ggctggtaca gctcgcgcag tccgatgcgg tgcaccacgc agtcccgcag cggacgcgcc
     1621 accagcgtgt gctgcggacc ggcgaccggc ccgagcagca ccgagcccgc ctccaggcgc
     1681 cccaggtggt gccagtggcc ctgctccccg gcgtcgaccg cgaacaggtc caccgcgccg
     1741 gacaccacca gccacagcac ctgcggcccc tccaggtcca gccggttgga cccggcgcag
     1801 tcgacgcgcg tgcccagcgc gccgagcgcc ccgaggacga ggtcgccctg ttcctcccgt
     1861 acggtcgtca tctcaccgct ccccgaccag cgccgcgtac gcgccaccgc gcgccaccag
     1921 ctcctcgtgc cgcccgcgct ccaccaccgt gccgtgctcc aggacgacga tctcgtcgct
     1981 gtcccgtacg gtgctgagcc ggtgcgcgat caccacgcag gcgcagccgc gccggcgcag
     2041 gttgtccatg accaccagct cggtctccgc gtccagcgca ctggtcacct cgtccagcac
     2101 gaggacgctg ggccggcgca ccagcgcccg cgcgatctcc aggcgctggc gctgcccgcc
     2161 ggagaagttc cggccgtcct gctcgacccg gctgtggacg ccgcccggcc tgcgcgtcac
     2221 cacgtcgtac agcgcggcgt ccctcagcgc ctccaccacg gcgtcgtcgg ggatcgacgg
     2281 gtcccacagc gccacgttgt cgcgcaccga gccctcgaac aggaacacgt cctggtcgac
     2341 gaaggagacg gaggacgcca gcgccccgcg cgggatgtcg tcgagccgct gcccgtccac
     2401 gcggatcacc ccgtcccagg gcgcgtacag gcccgagatc agccgcgaca ccgtcgactt
     2461 gccgctgccc gaaccgccca ccagcgccac ctgctgcccc ggcccgacgg tcaggtcgaa
     2521 accggtcagc agcggctcgt cgagcgggtt gtagccgaag gacacgttct gcaactcgac
     2581 gtggccgcgc agccggcggg tcgagtcggc gccgcccggg cggtcgtaga gcgggtccgc
     2641 ccggaagttc tccacgtcct tcagccgggc cacgtcggcc gcgaagtcct ggatgcggcc
     2701 cgcgacgccg ttgagccggg tcagcggggc cgtgaagcgg accaccaggg cctggaaggc
     2761 gaccagcaga ccgaccgaga tgtggccctc caccgcgcgc atgccgccga tccacaggat
     2821 cagcccgctg ttcagcgtcg cgagcgtcgg cgcgaccacg cccagccagg cgctcggcac
     2881 cccgagccgc tgctgctcct ccagcgtggt ggcgtgctgc ccggcccact tgcggaagta
     2941 gccgtcctcg ccgccggtcg ccttcatcgt ctcgatcagc tggaggccgg tgtacgcggt
     3001 gttggtcagc cgcgccgtgt ccgcccgcag cttcgccgta cgggtcgcgc gcagccggat
     3061 caccacccgc atcgcgagga tgttgagcag cgccacaccg atgccgacga aggtgagctg
     3121 cgggtcgtac gtgtacagca gcacggcgta caggacgacc acgatcgcgt ccacgcccgc
     3181 cgcggcgagg tcgcgggcca gcgtctcggc gaccgcgtcg ttggactgga ggcgctgcac
     3241 caggtcggcg gggctgcgct gggagaagaa ggtgaccgga agccgcagca ggtgccgcag
     3301 gaagcgggcg ctggacaggg tcgaggagat gatgcggccg tgcaggaggt tggcctgttg
     3361 cagccaggtc agcaccaccg tgagcagcac acaggcgccc atcgacgcga agagcacgcc
     3421 gagcagcgag gtctgcccgc cgatcaggaa caggtcgatg taggtacggc tgagcgcggg
     3481 gaccgccgcg ccgaccgcca ccaggagcag gctcgccagc acggccgcgg gcagcgtgcc
     3541 cgcggtgccg cgcagccggg cgggcatcgc gccgagcaca ccgggcctgc ggccgccgcg
     3601 ggtgaagccg tcgccgggca ccatggtcag cacgaccccg gtgaagctgc cgtcgaactc
     3661 ctccagggac acgaaacggc ggcccttgcc cgggtcgttg acgaacaccc cgcgccgccc
     3721 gaaccggcgg cccatgccgt cgtagacgac gtagtggttg aactcccaga agaggatggc
     3781 cggtgccgtc acctcggcga gcgcggccag gtccatctgc atgcccttgg ccgtgagccc
     3841 gtacctccgc gcggccttca gcaggttgct ggcgcgcgac ccgtcacggg agacaccgca
     3901 ggcgatgcgc agctcctcca gcgggacatg ccggccgtag tgccccagca ccatcgccag
     3961 ggaggcggcg ccgcactcca cggcctccat ctgcaacacg gtcggcgtac ggacggtctt
     4021 cgcgcggccc ttgggcacgc cgcgcccggg cggggcggcg cggcgtctgc cccgggtctc
     4081 ctgtgcggtg ctcacggcag cagccaatcg acgggacgcc ggtcggccag ccggatggag
     4141 cccgcggcca gggtcatgga ggtcagctcg aacggcggcc cgtcggccga ggaccactcg
     4201 tagccgctcc tggtggacgt cgattccgcc agttccaccg tcacggcgac cggcctgccg
     4261 tccttggtga actgctcgcc gagcgcgccg tccccgagga acgcaccgat cgtctgagcc
     4321 gactgggccg agcggtccac ggacttcacc tcgccgtgca ggacgccgta ccgctgcgtc
     4381 ggcaccgact gcacggtcag gtcgacggag gcgtgcgcgg ggatggcggc cgcgctctcg
     4441 gcgggcacgt agacggtggc gcggagcgga tcgtcggcgt gggcgacctt ctccaccgag
     4501 gcgacgttcg cgccggtggc gatgatctgc ccgacggtgg cggcgaggcc gctgacccgg
     4561 ccggcgtcga ccgagcggac cacggtctcg ccctcggagg tgcggacctt cagcaccggg
     4621 gcgtcggcgg ccagccgctg accggggcgc gcgaggaccg cggtgacctg ccccgcgacg
     4681 ggactctgca gcaggtaact gccctgcccg tgggtgagga cggcgggggc ggcgaccgtg
     4741 gaggtcaccg agccgcccac cgcccacacg gacgcggcgg ccatcgcgac cacggtcacg
     4801 gacagcgcca gccagccctg cgggcgggcg aaacgcaccg ggaggtcgag cccctccggt
     4861 gactggagtt tggtgagggc ctgttggcgg aactgcacga ctgacgtacc ccgggctcga
     4921 tgagaacggc cgaagaagga caacggccga aaagtcccgg agcctggccg gctccgggac
     4981 tcagcgacgc aagggcgcga tcagagaccg gcgaccaggc ccgtgaccgg ggccgtgttc
     5041 aggccggtga caccctcgac ggtgccgacg gccgtgttga gcaggccgga gaccggggcg
     5101 atgccgtcca ccagaccggt gacggtgccg gcggcgttca cgttcaggcc gccggagacg
     5161 ttgtcgaggt cggcgtcgga gatctcgacg gtctcgaccc ggggggtgga gttcatgatg
     5221 gaacttccct tcgtatagcc atttcacaag gggggagcgg ccccctctgg ggacagatga
     5281 cggccgcgac cgcgggcgcc gggcgcccgt ttccagagcc ctccgcggcc cccgcggtgc
     5341 gatggatcaa agcacgcggc cggtccgtgc ttccaatcaa tcacccctcc caccagcgca
     5401 cttgcgacga ccgggcgccc aacggtgcag gaccgcgcac gccgtcgggt cgacttcttc
     5461 acacggtgcg cgacatcggc ccgtgcgacc ccttgcccac gcggaagtga ttggggcact
     5521 cccgccccaa acgcgtaccg ggccccgccg ggttgtgcag atcctccagc gagggtgacc
     5581 gggttgggca atcgatgtgc agattcgctg aagcacacga tccgttccgc ggtctcaaca
     5641 gtgcgtcagc gaacgagtgc ggagcgtggg cggtcgacgg gacatgacga cccgtctgaa
     5701 caccccgccc ggtccgtgcg taccaacagc cgtccaggcg tccccacagc tgccggaccg
     5761 gcgggcacgg gccccggtcc caggaggtcg agcaggttga gtcaccagcg aattccgaag
     5821 cgcagggccg cgatggcggc aggcggtgtg gtggcgctcg gcgcggccgc gatcctgctg
     5881 cccaccgcca acgcctcgca gggcggcggc tccccggacg acgccgcgtc ggcgccccgg
     5941 accctcaagg cggcggacgc gtcgcagctc gcctcgcgcc tcgccggact gctcggcgac
     6001 accttcgcgg gctcctacta cgactccggc gcgcaacggc tcgtcgtcaa cgtcgccggc
     6061 ggggacgaca accccgtcgt cgcggacgcc gagaaggcgg gcgcggacgt ccgcgaggtc
     6121 gaccacagca tgggcgaact gcgcggcggc gcccggaccc tgaagtccga ggcgagcatc
     6181 cccggcacct cgtgggcgat cgacccgcgc accaacagga tcctggtgac cgccgacagc
     6241 accgtcaccg gcgaccggtg ggaccggctg gagtccaccg tcgagcgcct cggctccggc
     6301 atggcgaccg tcaggaagtc ggccggtacc ttcagaacgt tcgcgtccgg cggcgacgcc
     6361 gtcttcggcg gcggagcgcg ctgctcgctc ggcttcaacg tcaccgccgg cgacggctcg
     6421 cccgccttcc tgacggccgg tcactgcggg gtcgcggccg accggtggtc cgacgcgcag
     6481 ggcgggcagc cgacagccac cgtcgaccgg gccgtcttcc ccggggcggg cgacttcgca
     6541 ctcgtcacgt acgacgaccc ggcgaccgac gcccccagcg aggtgaacct cggcgaccgg
     6601 gccctcccga tcagccgggc cgcggaggcg acggtcgggc aggaggtctt ccgcatgggc
     6661 agcaccaccg gactggccga cggccaggtc ctcggcctcg acgccaccgt caactacccc
     6721 gagggcacgg tcaccggcct cgtacagacc gacgtctgcg ccgagccggg cgacagcggg
     6781 ggctccctgt tcaccgggga cggcctcgcg atcggtctga cctcgggcgg cagcggtgac
     6841 tgcacggtcg gcggcgagac cttcttccag ccggtcacca ccgcgctgga cgccgtgggc
     6901 gccaccctcg gcgacggcga cacaggcgcc ggtgacacgg gcgtggcgga cggcacgggc
     6961 gcaggtcacg aggacggctc cgggccgacg ggccgacggg ccgacgggct ggcgggcggg
     7021 aggtgacgcc ccgcaccgca gtgccccgcg cggtggcacg cagcgtggcc ggtccgaccc
     7081 gtaggccgcg cgcccggcgt ttcagggcgg ccccgccggg ggacccggag ggccgagcgc
     7141 ggcaacgcgc gaccggcgca accagaagga agatgatgct cgtgaccacg gacaccaccc
     7201 ggaaacccgc tgtccgcagg ctggagacag gctggtcgaa ggcccgtcgc ctgcggagca
     7261 ggggagtgat gcgtacctgc gtacccgccg tcgaggaccg cggaaccgcc cgcgcaccgc
     7321 acctgggcca ggagtcgaac atcgtccggg gcgaggactg aagcagcccc gagggcggac
     7381 accgcccggc gcgccggccc cgtcgccggc gcggtgcgcg ttccgggcag gtcacgaggc
     7441 cggtcccgga cggcgaagga cacgccggta gcgtcacggg cgtcccgcac cgcccgcccc
     7501 gaccgcagca aggcactgaa ccgttcatga acatcctggt caccggcgcc gccggattca
     7561 tcggatcccg gtacgtccgc acgctcctcg cgcgcgacgc gcccgacgag ccgcggatca
     7621 cggtgctcga cgccctcacc tacgccggga ccaccgccaa cctccaactc gaccacccac
     7681 ggctggagtt cgtgcacggc gacatccgcg acacggcgct cgtcgacaag ctggcggccg
     7741 aggccgacca ggtggtgcac ttcgccgcgg agtcccacgt ggaccgctcg atccacgccg
     7801 cggccgactt cgtcctcacc aacgtcgtcg gcacccagaa cctgctggac gccgccctgc
     7861 gccacggcgt gggcaccttc gtgcacgtct ccaccgacga ggtctacggc tccgtcgaga
     7921 cgggctcggc gaccgaggaa cacccgctgc ggcccagctc gccgtactcc gcctcgaagg
     7981 cgtcgggcga cctgctggcc ctctcgtacc accgcaccca cggcctcgac gtccgggtga
     8041 cccggtgctc caacaactac ggcccgcacc agttccccga gaagctcgtg ccgctgttcg
     8101 tcacccacct cctggacggc cgcagggtcc ccctctacgg cgacggacgc aacgtccgcg
     8161 actggctgca cgtcgacgac cactgccggg gcgtcgacct cgtgcggacc cggggccggg
     8221 ccggcgaggt ctacaacatc ggaggcggca cggagttgag caacctcgac ctcaccggcc
     8281 tgctcctcga cgcctgcgac gcgggcccgg accggatcgt gcacgtcgcg gaccgcaagg
     8341 gccacgacct gcgctactcg gtggactgga gcaaggcccg cgaggagctg ggctaccgcc
     8401 cccggcacga cttcccggcc ggactggccg agaccgtcgc ctggtaccgg gacaaccgcg
     8461 cctggtggga gccgctcaag cggcgcaccg acgtggcgtg agaggcgtcc cgcccggtca
     8521 gcagtgcgtc ttggccagca gctccaggac ctcccgcgtc gtgcccgtct cgcccaggcg
     8581 cgggaagatc cgctcgacgc tgccccggtg cgcctgtgcg ctcatgtcgc tcatggcgtc
     8641 ggtggcgagc gtgacgtggt agccgtgctc gtgggcggct cgggcggtgg actccacgcc
     8701 gatgctggtg gcgatgccgc agagcacgat ctgggtgacg ccgcggcgac gcagctgcac
     8761 gtcgagaccg gtgccgtgga aggcgcccca gttgcgcttg gtgacggtga tgtcgccggg
     8821 gtgcccggcc agttcaccga ccacgacgtc ccagccctcg ggccgcggac caccggcccc
     8881 tcccgcctcc gtacggccgg gcacggcgtc cgccccgtcg gcggcgaagg agacccggac
     8941 cagtaccacg ggcaggtccc gggcccggaa ggcttcggcc agttcggcac tccgggcgac
     9001 gacctcgggg ccggtgtacg gctcggtggg cgcggcgacg ataccggcct ggaggtcgat
     9061 gacgacgagg gcggtgcggg ggtcgagggt ggtgacgctc atgacctcac cgtaaattcc
     9121 acagccaaga ctgtccagct aaactgaaca gttttacctg ctgattacgg gggcgccgcc
     9181 ggccctccgg cgctaggctg gccccgtgcg tctgctgatc atgtccgaca cccacctgcc
     9241 caagcgggcg aaggaactgc cggcgccgct ggtggcggag atcccgcgcg cggacgtcgt
     9301 cctgcacgcg ggggactggg tcgacacggc caccctcgac ctgctggaga gccgcagccg
     9361 cagactcgtc ggcgtccacg gcaacaacga cggacccgcg ctgcgcgcca gactgcccga
     9421 ggtggcgtac gcggatctcg gcggcctgcg tttcggcgtg gtccacgaga ccggcgccgc
     9481 acaggggcga gaggcacggt gcgccgcccg gttccccggc ctcgacgtcc tcgtcttcgg
     9541 gcacagccac atcccctggg acaccacggc cgccaccggc ctgcggctgc tcaacccggg
     9601 atcgcccacg gaccgccgcc gtcagccgta ccgcacctac atgacggcca cggtcaccga
     9661 cggccggctc ggggccgtcg aactgcaccg gctgccgacg gggtgaacgc gcagcggtgc
     9721 ggagcagtcc ggccgccccg gacccgaccg aacacccgga ctactcgtcc caggcctgga
     9781 tcatggtctg ctcgacgatc tcgccgtccc gcagcgacag catcgactcg gcgaggaccc
     9841 gcaccccgtc cggacaggtg cactcctcgg tgaacgcgac ctggtcgccc tggaccacgc
     9901 agcgttccat ccggtgggtc atgtcgcggc tgtagacgtc gtcgagcatc tcggcgatct
     9961 cgtcccggcc gtgcagcacc ttcggacggc tcggctggtc gtaccggtcg acgacgcgga
    10021 cccgcgcgtc ccgcgcgtac agcgacagca ggggtgccgc cgtcttcccc tcgatgcccc
    10081 ggcgcagtgc ttcggtgtcg aagccggggt ctgccgtggt gctcatgacg acctccttcg
    10141 cacgtcgagg gccgcggccc ggagcgggaa cgggccgccc ggccctctcc ttcgagcttc
    10201 ctccgtcccg gcgccc
//
