LOCUS       NZ_CP116257            11368 bp    DNA     linear   CON 15-DEC-2024
DEFINITION  Streptomyces lienomycini strain DSM 41475 chromosome, complete
            genome.
ACCESSION   NZ_CP116257
VERSION     NZ_CP116257.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces lienomycini
  ORGANISM  Streptomyces lienomycini
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7863969)
  AUTHORS   Ding,W.
  TITLE     Streptomyces riduolalong sp. nov., isolated from the soil of Tibet
            exhibits against Gram-positive bacteria activity
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7863969)
  AUTHORS   Ding,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) School of life sciences and biotechnology,
            Shanghai Jiaotong University, 800 Dongchuan, Shanghai, Shanghai
            200240, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: GS De Novo Assembler v. August-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_027947595.1-RS_2024_12_13
            Annotation Date                   :: 12/13/2024 16:03:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,183
            CDSs (total)                      :: 7,093
            Genes (coding)                    :: 6,850
            CDSs (with protein)               :: 6,850
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 243
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 243
            Pseudo Genes (frameshifted)       :: 141 of 243
            Pseudo Genes (incomplete)         :: 121 of 243
            Pseudo Genes (internal stop)      :: 14 of 243
            Pseudo Genes (multiple problems)  :: 26 of 243
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:34:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2215097
            Orig. end                         :: 2226465
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116257.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11368
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="6"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11368
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11368
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6368](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6368)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            160..1644
                     /gene="hemG"
                     /locus_tag="BJ961_RS10060"
     CDS             160..1644
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445289.1"
                     /locus_tag="BJ961_RS10060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_271320976.1"
                     /transl_table=11
                     /translation="MSASETPAGEFPADGGQGHVVVVGAGIAGLAAAHRLLERGTRVTV
                     LEASGRVGGKLLPGEIAGVRVDLGAESMLARRPEAVGLARAAGLADRLQPPSTATASLW
                     TRGVLRPMPKGHVMGVPGTAAALSGVLSEEGLARIGRDAELPPTEVGDDVAVGEYVAAR
                     LGREVVDRLVEPLLGGVYAGDAYRISLRSAVPQLFEAARTHTSLTEAVRTLQGRTATSP
                     PSGPVFMGIEGGIGTLPPAVAESVRARGGEIVTHAPVTELRRTACDGWRIVAGDRTLHA
                     DAVVVAVPAGPAAALLRAEAPAAAAELSAVEYASMALITLAYRRSDAAALPEGSGFLVP
                     PVDGHTIKASTFASRKWGWIADEDPDLVVLRTSVGRYGDTEILGRDDEGLVDVSRHDLA
                     QATGLIAAPVATRVTRWHDGLPQYPVGHHARVARVREHVARLPGLAVCGAAYDGVGIPA
                     SIASAYAAADLIHADPRGVQELTGHPVESRHGGAGE"
     gene            1649..2377
                     /gene="hemQ"
                     /locus_tag="BJ961_RS10065"
     CDS             1649..2377
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="BJ961_RS10065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_271320977.1"
                     /transl_table=11
                     /translation="MSDDATTPADRIPNKGKLAKDLNEVIRYTLWSVFKLKDALPEDRA
                     GYADEVQELFDQLAAKDVTVRGTYDVSGLRADADLMIWWHAETADQLQEAYNLFRRTRL
                     GRALEPVWSNMALHRPAEFNRSHIPAFLADEQPRNYVSVYPFVRSYDWYLLPDEDRRRM
                     LADHGKMARGFPDVRANTVPAFSLGDYEWVLAFEADELHRIVDLMRHLRGSEARMHVRE
                     EIPFYTGRRKDVAELVAGLA"
     gene            complement(2378..3961)
                     /locus_tag="BJ961_RS10070"
     CDS             complement(2378..3961)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (110-485). E-value:
                     1.4e-19. Score: 62.6. Matches aSDomain:
                     nrpspksdomains_BJ961_RS10070_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006380016.1"
                     /locus_tag="BJ961_RS10070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_271320978.1"
                     /sec_met_domain="PF00561 (E-value: 2.6e-23, bitscore: 81.7,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAVLHSAAGSLLLTTLAVAPARAAPSEGPGSAEARGTAVAAGR
                     AAAAGIEFGRCPKAEDLPGAIRCGTVTVPLDYAHPDGKRIELTVSRVRATHEDPDGSDR
                     EVPGQGALLYNPGGPGASGLYFPLVGMVPEWKRIAAAYDLVGYAPRGVAPSAPLSCQDP
                     GDFFKGPRPAPAQPSQAYKRQRAAEAEAYANDCAERGGDALRHYHSLNNARDLEVVRAA
                     LGERRLTFMGASYGTYLGALYAELFPSHVRRMVFDAVVDPDPEQVWYRNNLEQSEAFED
                     RWDDFKEWVAEHDAVYGLGDRPRKVQDAYEEASERLAAEPAGGTVGPAQLQGAMLKAGY
                     YDDYWPHRAEALSAYLKGDPQPLIDQAGTRTEAGAVAAENSNAVYTAVECNDAPWPTDF
                     EVWDRDNTRLARRAPFETWDNVWTNLPCAYWRAPRQRPLDVRTAPGELPPTLILAAERD
                     AATPYDGALELRRRLSGSVLVTERDAGTHGIGGGPNACVNAHLEAYLLDGRLPARDASC
                     APRPEPEPRA"
     aSDomain        complement(2507..3631)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BJ961_RS10070_Abhydrolase_1.1"
                     /evalue="1.40E-19"
                     /label="BJ961_RS10070_Abhydrolase_1.1"
                     /locus_tag="BJ961_RS10070"
                     /protein_end="485"
                     /protein_start="110"
                     /score="62.6"
                     /tool="antismash"
                     /translation="ALLYNPGGPGASGLYFPLVGMVPEWKRIAAAYDLVGYAPRGVAPS
                     APLSCQDPGDFFKGPRPAPAQPSQAYKRQRAAEAEAYANDCAERGGDALRHYHSLNNAR
                     DLEVVRAALGERRLTFMGASYGTYLGALYAELFPSHVRRMVFDAVVDPDPEQVWYRNNL
                     EQSEAFEDRWDDFKEWVAEHDAVYGLGDRPRKVQDAYEEASERLAAEPAGGTVGPAQLQ
                     GAMLKAGYYDDYWPHRAEALSAYLKGDPQPLIDQAGTRTEAGAVAAENSNAVYTAVECN
                     DAPWPTDFEVWDRDNTRLARRAPFETWDNVWTNLPCAYWRAPRQRPLDVRTAPGELPPT
                     LILAAERDAATPYDGALELRRRLSGSVLVTERDAG"
     gene            complement(4077..4874)
                     /locus_tag="BJ961_RS10075"
     CDS             complement(4077..4874)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972877.1"
                     /locus_tag="BJ961_RS10075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_271320979.1"
                     /transl_table=11
                     /translation="MFWVLFLLSAWVVAGLACLRLCLAAVRAAAVVAPAAAREHALTLY
                     EAAFLSGGPRRVADLTLVSMARQRRLLLAHTGWATVVDPRGRDDMERSVIGAIGPGGQS
                     RIAPVRATAATAEAVRGLADRLVGAGLAVPDGGAGGVAAGVRRVRAAAVSVCALGLVAL
                     ALPLPATEPRPLIALWFALPLALTLGCLALTRLEVHPYSRWASPAGQRLIGALVRQADG
                     TADDRTFLTSVAVRGVHAIGEPDLRAAFAHRERYAGDQWERRA"
     misc_feature    4936..4953
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 13.84"
                     /tool="antismash"
     gene            complement(5001..6368)
                     /locus_tag="BJ961_RS10080"
     CDS             complement(5001..6368)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016327585.1"
                     /locus_tag="BJ961_RS10080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_271417009.1"
                     /sec_met_domain="DUF692 (E-value: 4.2e-98, bitscore: 326.5,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MARLGTGVGWRPEIADVVESMPGIDWVEAVSENLCPGHLPDSLLR
                     LRERGVTVVPHGVSLGLGDARRPDAGRLAALAERAEVLGSPLVTEHIAFVRAGGALTAS
                     PPLEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLAVENIAALVSWPGEEMTEGQFLY
                     ELADRTGVRLLIDVANLHTNHVNRGEDPAEALAELPLEALAYVHVAGGFERDGVWHDSH
                     AHPVPRPVLDILTDLASRVAPPGVLLERDDNFPAGHELEGEVAAIRTAVAKGRAAGPAA
                     TGDRAAPPASRAGAGSGASAGAGAGAGAGAGAGADDAVRQRVGLAQAALLAALVAGAPV
                     PEGFDGVRLGVQARALAGKRADVVAKVAPELPVLLGADYRPAFLAYAEGRPMTGGYRRD
                     ALDFAEHLLRTAPGGAAPGVRRAVREWWLERSGPVPRSARPGVRLARATRRVLLGR"
     gene            6499..7554
                     /locus_tag="BJ961_RS10085"
     CDS             6499..7554
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018893769.1"
                     /locus_tag="BJ961_RS10085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF2804 domain-containing protein"
                     /protein_id="WP_271320980.1"
                     /transl_table=11
                     /translation="MATHEREITGPVDLCLPDGRLDPAAVGWSRVPLHRANLRGWGRTK
                     RWEYWCVTTPTHLVALTVSDLDYLALNTVYLLEYAAAGREFERAAIVPAGRGVRLPDTV
                     AGTPGAGDVVVGPERPTRGKVRVEIRDEHGGTRLRARCLDPDRLPVEVDLLVARPEGHE
                     SLSVVVPWSERRFQYTSKHTALPAAGRVRIGTEVLGFGGDDGEAWAVLDHGRGRWPRTV
                     DWNWGAASGRTDGHTVGLQFGGRWTAGTGATENGLCVDGRLTKIGEELDWRRSPSGPQA
                     PWTIRTPSSDQVDLTFTPFHNRSAHTNAGLVVNRTDQRFGHYDGRIRTDDGTRIAVNGL
                     LGWAEDVHMRW"
     gene            complement(7558..8304)
                     /locus_tag="BJ961_RS10090"
     CDS             complement(7558..8304)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445276.1"
                     /locus_tag="BJ961_RS10090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_271320981.1"
                     /transl_table=11
                     /translation="MSNDPTTPVPAPVSAPDSPFRPEPGDRDLAPQFVLPLVVRIERAA
                     PPPRTDALETAARAVLVMLADERSAGDGEWARAMRDWQDARIRKVVRRARGAEWRRAGA
                     LPGVTVTGRTAEVRVFPPVPLDGWPKDLARLQVSGTDLADPEPPLDADPSAPVLWLNPG
                     LDMSAGKAMAQAGHGAQLAWWELSGAERAAWRDAGFPLSVRTADPARWDGLTTGGLPVV
                     RDAGFTEIAPGSCTVVADHPALARIE"
     gene            complement(8379..8573)
                     /locus_tag="BJ961_RS10095"
     CDS             complement(8379..8573)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125244.1"
                     /locus_tag="BJ961_RS10095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_271320982.1"
                     /transl_table=11
                     /translation="MSDDDRGSDDYCLIDAVRPPGAAGPPYADCVLCREPTEYPESYKG
                     ITLCPVCEWQEAQRTACSG"
     gene            complement(8592..9470)
                     /locus_tag="BJ961_RS10100"
     CDS             complement(8592..9470)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011030618.1"
                     /locus_tag="BJ961_RS10100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_271320983.1"
                     /transl_table=11
                     /translation="MITLRRLRRATALCVLGAALAGCGTTEAGRQAPRPAPSAPAAPSP
                     SRTPTLAPGPGGLTPVFEHGPRTDRDKAGAPDGKLVALTFDADMTADQGPRAAAGEHFD
                     NPRLIATLRKLKVPATVFMTGRWAEEYPDEARSIGRDPRFEIANHSYSHYAYTDDCYGL
                     PTVSEDRMRSDLERAYTAFEKAGVANPMPYFRFPGGCYDKAALKELAATGVTAVQWDVV
                     SGDAFATDADAVTRQVLDGVRPGSVVVMHCTRSAAPVTEEVVRAVVPELRRQGYRFVKV
                     SELIGAANTHR"
     misc_feature    9500..9513
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.19"
                     /tool="antismash"
     gene            complement(9560..11083)
                     /locus_tag="BJ961_RS10105"
     CDS             complement(9560..11083)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020133759.1"
                     /locus_tag="BJ961_RS10105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_271320984.1"
                     /sec_met_domain="DAO (E-value: 3.3e-48, bitscore: 163.7,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQNVQVNGSYWLQTAPGGAPRPALAEDLDVDVAVIGAGVAGLST
                     AWELARAGRGVAVLEAGRVAAGVTGYTSAKLTAQHTLVYDKLRRTRGPEGARLYARSQS
                     EAIERAAGIVAELGIDCDWETRDAYTYVRDPGRVEELRAEAAAAKEAGLPASFVTETGL
                     PFPVAGAVKVTGQAQFHPVKYLLALAADLEARGGRIFEDTTVQGLDEGEPCRVRTEAGA
                     TVTARDVVVATHYPVFDRALLFARLSPRRELVVAGTVDADRDVDGMYITPEENTRSVRT
                     APLTDPGRRLLIVTGEHFTPGTGDTRERFETLAAWADEHFPGADRTHAWATQDIDPTDT
                     VPMVGPLHQGARHAYVATGFGGWGLSGGMMAGRLLTAQISGEECAWSGLYDPRRLRTAV
                     REAPALLRTQAEVARHFVGDRLRSAPPVEALPPGEGAVVRAGGDRVAVYRDEEGALHAV
                     SPRCTHLGCLVDFNAAERAWECPCHGSRFDTDGKVVEGPATKPLEQRDI"
ORIGIN
        1 tcggtgacaa ctgacgtctc tacgacgcgc gaaggggcgc ggacgacggc gcggaacggt
       61 tccgaagaga tcacggtccg gactcgcggg acctgtgggc cggaccacgg ggccacccgg
      121 ccccgggtcc gggcgggcgg ggtctgagac gctggggaca tgagcgcgag tgagaccccg
      181 gcgggcgagt tccccgcgga cgggggacag ggacatgtcg ttgtcgtggg agccggcatc
      241 gccggactgg ccgccgccca ccggctgctg gagcgcggca cccgggtgac cgtgctggag
      301 gcgtccggcc gcgtcggcgg caaactgctg cccggcgaga tcgccggagt gcgcgtcgac
      361 ctcggcgccg agtcgatgct ggcccgccgc cccgaggccg tcggtctggc ccgcgcggcc
      421 ggactcgccg accgcctcca gccgccgtcc accgcgacgg cctccctgtg gacccgcggc
      481 gtcctgcgcc ccatgcccaa gggccacgtc atgggcgtcc ccggcaccgc cgccgccctg
      541 tccggcgtcc tctccgagga gggcctcgcc cgcatcgggc gcgacgccga actgcctccc
      601 accgaggtcg gcgacgacgt ggccgtgggc gagtacgtcg ccgcgcgtct gggccgcgag
      661 gtcgtcgacc ggctcgtcga gcccctgctg ggcggcgtct acgcgggcga cgcctaccgc
      721 atctccctgc gctccgccgt gccccagctc ttcgaggccg cccgcaccca cacctcgctc
      781 accgaggcgg tccgcacgct ccagggccgc accgccacct ccccgccgag cggcccggtg
      841 ttcatgggca tcgagggcgg catcggcacc ctcccgcccg ccgtcgccga gtcggtgcgc
      901 gcgcggggcg gcgagatcgt cacgcacgcg cccgtcaccg agctgcgccg cacggcctgc
      961 gacggctggc ggatcgtcgc cggcgaccgg accctgcacg ccgacgcggt cgtcgtcgcc
     1021 gtccccgccg gtcccgccgc cgcactgctg cgcgccgagg cgcccgccgc cgcggccgag
     1081 ctgtccgcgg tggagtacgc ctcgatggcc ctgatcaccc tcgcctaccg ccgctccgac
     1141 gccgccgccc tccccgaggg cagcggcttc ctggtgccgc cggtcgacgg gcacaccatc
     1201 aaggcgtcca ccttcgcctc ccgcaagtgg ggctggatcg ccgacgagga cccggacctg
     1261 gtcgtcctgc gcacctcggt gggccggtac ggggacacgg agatcctcgg ccgcgacgac
     1321 gagggcctcg tcgacgtctc ccggcacgac ctggcgcagg ccaccggcct gatcgccgcc
     1381 cccgtcgcca cccgcgtcac ccgctggcac gacggcctgc cgcagtaccc cgtcggccac
     1441 cacgcgcgcg tggcccgcgt ccgcgagcac gtcgcgagac tccccggcct cgcggtgtgc
     1501 ggcgcggcct acgacggcgt cggcatcccg gcgagcatcg cgagcgcgta cgccgccgcc
     1561 gacctgatcc acgcagaccc gcgaggtgtg caggaactca ccggccaccc ggtggagagc
     1621 cgccacggcg gagcgggaga ataagggcat gagtgacgac gccaccaccc ccgccgaccg
     1681 gatccccaac aagggcaagc tggccaagga cctcaacgag gtcatccgct acaccctctg
     1741 gtccgtcttc aagctcaagg acgcgctgcc cgaggaccgc gcgggctacg ccgacgaggt
     1801 ccaggagctg ttcgaccagc tcgccgccaa ggacgtgacc gtccgcggca cctacgacgt
     1861 gtccggtctg cgcgccgacg ccgacctcat gatctggtgg cacgccgaga ccgccgacca
     1921 gctccaggag gcgtacaacc tcttccgccg caccaggctg ggccgtgcgc tggagccggt
     1981 gtggtcgaac atggcgctgc accgccccgc cgagttcaac cgctcgcaca tcccggcctt
     2041 cctcgccgac gagcagcccc ggaactacgt cagcgtctac ccgttcgtac gctcgtacga
     2101 ctggtacctg ctgcccgacg aggaccgccg ccgcatgctc gccgaccacg gcaagatggc
     2161 ccgcggcttc ccggacgtcc gcgccaacac ggtgcccgcg ttctccctcg gcgactacga
     2221 gtgggtcctc gccttcgagg ccgacgaact gcaccgcatc gtcgacctca tgcgccacct
     2281 gcgcggctcc gaggcccgca tgcacgtccg cgaggagatc ccgttctaca ccgggcgccg
     2341 caaggacgtc gcggagctgg tggccgggct ggcctagtca ggccctgggc tcgggttccg
     2401 ggcgcggggc gcaggaggcg tcccgcgccg ggaggcggcc gtccagcagg tacgcctcca
     2461 ggtgggcgtt gacgcaggcg ttggggccgc cgccgatgcc gtgcgtcccc gcgtcccgct
     2521 cggtcaccag gaccgagccc gacaggcgcc gccgcagctc cagcgcgccg tcgtacgggg
     2581 tggccgcgtc gcgttcggcg gccaggatca gcgtcggcgg cagctcaccg ggcgcggtgc
     2641 gcacgtccag gggccgctgc cggggcgccc gccagtaggc gcacggcagg ttcgtccaca
     2701 cgttgtccca ggtctcgaag ggcgcccggc gggccagccg ggtgttgtcc cggtcccaga
     2761 cctcgaagtc ggtcggccag ggcgcgtcgt tgcactcgac ggccgtgtac acggcgttgg
     2821 agttctcggc ggcgaccgcc cccgcctcgg tccgggtgcc cgcctggtcg atcagcggct
     2881 gcgggtcgcc cttcagatag gccgacaggg cctcggcgcg gtgcggccag tagtcgtcgt
     2941 agtacccggc cttcagcatc gcgccctgca actgcgcggg ccccaccgtc ccgcctgcgg
     3001 gctccgcggc cagccgctcg ctcgcctcct cgtaggcgtc ctgcaccttc ctcggccggt
     3061 ccccgagccc gtagacggcg tcgtgctcgg cgacccactc cttgaagtcg tcccagcggt
     3121 cctcgaaggc ctccgactgc tccaggttgt tccggtacca gacctgttcc ggatccgggt
     3181 ccaccaccgc gtcgaacacc atccgccgta cgtgcgacgg gaacagctcg gcgtacagcg
     3241 ccccgaggta ggtgccgtac gacgcgccca tgaacgtgag ccggcgttcg cccagcgccg
     3301 cgcggacgac ctccaggtcg cgggcgttgt tgagggagtg gtagtgccgc agcgcgtccc
     3361 cgccccgttc cgcgcagtcg ttcgcgtacg cctcggcctc ggcggcacgc tgcctcttgt
     3421 acgcctgcga gggctgcgct ggggcgggcc ggggcccctt gaagaagtcg ccggggtcct
     3481 ggcaggacag cggcgcggag ggcgcgaccc cgcggggggc gtacccgacg aggtcgtagg
     3541 ccgcggcgat ccgcttccac tcgggcacca tgccgaccag cgggaagtac aggcccgagg
     3601 cgccggggcc gcccgggttg tagaggaggg cgccctggcc ggggacttcg cggtcgctgc
     3661 cgtcggggtc ctcgtgggtg gcccggacgc ggctgacggt gagttcgatc cgcttcccgt
     3721 cggggtgcgc gtagtccagg ggcacggtga ccgtgccgca gcggatggcg cccggcaggt
     3781 cctccgcctt cgggcacctg ccgaactcga tcccggccgc cgccgcgcgc ccggcggcca
     3841 cggcggtgcc gcgcgcctcg gcggaacccg gcccctcgga cggcgccgcc cgggccgggg
     3901 cgacggcgag ggtggtcagc agcaaggacc cggcggccga gtggaggacg gcagctcgca
     3961 tcgcgtatcc cttcggtgca cggcgtgaca ccagggatgc tcggtgcggc gggcgggcgg
     4021 tacaagcacc gcccgcgggt gtcggcgccg agtgcctcca tgcgcccccc ggcgcgtcag
     4081 gcccggcgct cccactggtc cccggcgtac ctctcgcgat gggcgaaggc ggcacgcagg
     4141 tccggttcgc cgatcgcgtg cacgccccgc acggcgaccg aggtgaggaa cgtacggtcg
     4201 tccgccgtgc cgtcggcctg ccggacgagg gcaccgatca gccgctgccc ggccggggag
     4261 gcccagcgtg agtacgggtg gacctccagc cgcgtgaggg cgaggcagcc cagggtcagg
     4321 gcgagcggca gcgcgaacca gagggcgatc agcgggcgcg gttccgtggc gggcagcggc
     4381 agcgccaggg cgaccaggcc gagcgcgcac acgctcaccg cggcggcccg cacccgccgc
     4441 accccggccg ccacgccacc ggcgccgccg tcgggtacgg cgaggcccgc gccgaccagc
     4501 cggtcggcca gcccccgcac cgcctccgcg gtggccgcgg tggcccgcac cggcgctatc
     4561 cgggactgcc ccccggggcc tatcgcccct atgaccgacc gctccatgtc gtcccgcccg
     4621 cgcgggtcga cgacggtcgc ccagccggtg tgcgcgagca gcagccgacg ctggcgtgcc
     4681 atggagacca gtgtcaggtc ggccacccgg cgcgggcccc cggacaggaa cgccgcctcg
     4741 tacagcgtca gcgcgtgctc ccgggcggcg gcgggcgcga cgacggccgc cgcgcgcacg
     4801 gcggccaggc acagccgcag gcaggcgagg cccgcgacga cccaggccga caggaggaag
     4861 aggacccaga acatgccgta tgtctatgcc aaagggtttc gccggcgcca tgccctgttc
     4921 acattccggg acacggtgtt gcgggtgtgt gacgttccgt ttggtcccgt tccgtgctgc
     4981 cccccgtggg tgtctccgcg tcagcggccc agcaggacgc gccgggtggc gcgggccagc
     5041 cggaccccgg gacgcgccga ccgcggcacc ggccccgacc gctccagcca ccactcgcgc
     5101 accgcccgcc gcacccccgg ggccgcgccc ccgggcgccg tccgcagcag gtgctcggcg
     5161 aagtcgaggg cgtcgcgccg gtatccgccg gtcatcgggc gcccctcggc gtaggcgagg
     5221 aaggccggcc ggtagtcggc gcccaggagg accggcagtt cgggggcgac cttcgcgacc
     5281 acgtccgcgc gcttcccggc gagcgcccgc gcctgcaccc ccagccgtac cccgtcgaac
     5341 ccctcgggga cgggcgctcc cgcgaccagc gccgccagca gcgcggcctg cgccagcccg
     5401 acccgctgcc gtacggcgtc gtcggcaccg gcaccggcac cggcaccggc accggcaccg
     5461 gcaccggcac tggcaccgga accggcaccg gcgcgcgagg cgggcggtgc cgcccggtcg
     5521 ccggtggcgg ccgggcccgc cgcccgcccc ttcgcgacgg ccgtgcggat cgccgccacc
     5581 tcgccctcca gctcgtgccc tgcggggaag ttgtcgtcgc gttccagcag gacacccggc
     5641 ggtgccacgc gcgaggcgag gtcggtcagg atgtcgagga ccggccgcgg gacgggatgg
     5701 gcgtggctgt cgtgccagac gccgtcccgc tcgaagccgc ccgcgacatg aacgtaggcg
     5761 agggcctcca ggggcagttc ggcgagcgcc tcggcggggt cctcgccgcg gttgacgtga
     5821 ttggtgtgca ggttcgccac gtcgatcagc agccgcaccc ccgtacggtc ggccagctcg
     5881 tacaggaact gtccctccgt catctcctcg cccggccagg agacgagcgc ggcgatgttc
     5941 tccacggcga gcggcaccgg cagcgcgtcc tgcgcgatgc gcacattctc gcagagcacg
     6001 tcgagggcgt cgcgggtgcg cggcacgggc agcaggtgtc ccgcctccag cggcggcgag
     6061 gcggtcagcg ccccgcccgc ccggacgaac gcgatgtgct cggtgaccag cggcgacccc
     6121 aggacctccg cccgctcggc cagcgccgcc agccgccccg cgtccggccg ccgggcgtcg
     6181 ccgagcccga gcgagacgcc gtgcggcacg acggtcaccc cgcgctcgcg cagccgcagc
     6241 agcgagtcgg gcagatgccc gggacagagg ttctcggaca cggcctcgac ccagtcgatg
     6301 ccgggcatcg actcgacgac gtccgcgatc tccggccgcc atccgacacc ggtgcccagt
     6361 cgcgcgatgg atcgcatgat cgcgtccccc tttcctgcgc acatgctcgg ggtatcgccc
     6421 acccggtccg gtgccaaccc cggggagggc cggttcagag gaacatctga ggttcgcgcc
     6481 gcccagtagg ctccggccat ggcgacgcac gagcgcgaga tcaccggacc cgtcgacctc
     6541 tgcctgcccg acggccggct cgacccggcg gccgtcggct ggtcccgggt gccgctgcac
     6601 cgggcgaacc tgcggggctg gggtcgcacc aagcgctggg agtactggtg cgtgaccacg
     6661 cccacccacc tggtggccct gaccgtcagc gacctcgact acctcgccct gaacacggtg
     6721 tacctgctgg agtacgcggc ggcgggccgc gagttcgagc gcgccgcgat cgtcccggcc
     6781 ggccgcggcg tccgcctccc ggacaccgtc gccggcaccc cgggcgccgg ggacgtcgtc
     6841 gtgggccccg agcggccgac ccggggcaag gtgcgcgtgg agatccgcga cgagcacggc
     6901 ggtacgcgcc tgagggcccg ctgcctcgat ccggaccggc tgcccgtcga ggtcgacctg
     6961 ctggtcgcgc ggcccgaggg ccacgagtcg ctctcggtcg tggtgccgtg gagcgagcgg
     7021 cgcttccagt acacgtccaa gcacaccgcc ctccccgccg ccggccgcgt gcggatcggc
     7081 accgaggtcc tcggcttcgg cggggacgac ggcgaggcct gggcggtgct ggaccacggg
     7141 cggggccgct ggccgcgcac ggtcgactgg aactggggcg ccgcgtccgg ccgcacggac
     7201 gggcacacgg tgggactcca gttcgggggg cgctggaccg cgggcaccgg ggcgaccgag
     7261 aacggcctct gcgtggacgg ccgcctcacc aagatcggcg aggagctgga ctggcgccgg
     7321 tcgccctccg gcccgcaggc cccctggacg atccgtacgc cgtcgtccga ccaggtcgac
     7381 ctgaccttca cgccgttcca caaccgctcc gcgcacacca acgccggcct cgtcgtcaac
     7441 cgcaccgacc agcgcttcgg ccactacgac ggccggatcc gcaccgacga cggcacgcgc
     7501 atcgccgtga acgggctcct gggctgggcc gaggacgtcc acatgcgctg gtgaccgtca
     7561 ctcaatacgg gcgagcgcgg ggtggtccgc caccaccgtg caggagcccg gcgcgatctc
     7621 cgtgaacccg gcgtcgcgca ccacgggcag gccgccggtg gtgaggccgt cccagcgggc
     7681 ggggtccgcg gtgcgcaccg acagcgggaa ccccgcgtcg cgccacgccg cccgctcggc
     7741 tcccgacagc tcccaccagg cgagctgcgc accgtggccc gcctgggcca tcgccttgcc
     7801 cgccgacatg tccaggccgg ggttcagcca cagcaccggc gccgacgggt ccgcgtcgag
     7861 cgggggttcg gggtcggcga ggtcggtgcc ggagacctgg aggcgggcca ggtccttcgg
     7921 ccagccgtcc agcgggaccg gcgggaagac ccgcacctcg gcggtcctgc cggtgaccgt
     7981 gacgccgggc agcgccccgg cccgccgcca ctcggcgccg cgcgcccgcc gcacgacctt
     8041 gcggatccgc gcgtcctgcc agtcccgcat cgcccgcgcc cactcgccgt ccccggccga
     8101 ccgctcgtcg gcgagcatca ccagcaccgc ccgggccgcg gtctccagcg cgtcggtacg
     8161 gggcgggggc gccgcccgct cgatgcgtac gaccagcggc aggacgaact gcggcgcgag
     8221 gtcgcggtcg ccgggctcgg gacggaaggg gctgtcgggg gcggagacgg gggcggggac
     8281 gggcgtcgtc ggatcgttgc tcacgcccta cagtctgcca acccggcgcc gggccccgct
     8341 cgccgccgcc gcgaccgacc gcgctcggcc cgcgcaggtc agcccgagca ggccgtgcgc
     8401 tgggcctcct gccactcgca gaccgggcag agggtgatgc ccttgtacga ctccgggtac
     8461 tccgtcggct cccggcacag gacgcagtcg gcgtacggcg gtccggcggc cccgggaggc
     8521 cggaccgcgt cgatcaggca gtagtcgtcc gaaccccggt cgtcgtcgct catgcctcca
     8581 gggtaggccc gtcagcggtg cgtgttcgcg gcgccgatca gctcggagac cttcacgaag
     8641 cggtacccct ggcggcgcag ctcgggcacc accgcccgca ccacctcctc ggtcaccggg
     8701 gcggcgctgc gcgtgcagtg catgacgacg accgaaccgg gccgcacccc gtcgaggacc
     8761 tgccgggtca ccgcgtcggc gtccgtggcg aacgcgtccc cgctcaccac gtcccactgc
     8821 acggcggtga cgccggtggc ggccagttcc ttgagcgccg ccttgtcgta acagccgccg
     8881 gggaagcgga agtacggcat cgggttcgcc accccggcct tctcgaacgc cgtgtacgcg
     8941 cgctccaggt ccgaccgcat ccggtcctcg gagacggtgg gcaggccgta gcagtcgtcg
     9001 gtgtaggcgt agtggctgta ggagtggttg gcgatctcga agcgcgggtc gcggccgatg
     9061 gaacgggcct cgtccggata ctcctcggcc cagcggccgg tcatgaacac cgtggcgggc
     9121 accttcagct tccgcagggt cgcgatcagc cgggggttgt cgaagtgttc gcccgccgcc
     9181 gcccgcggcc cctggtcggc ggtcatgtcg gcgtcgaagg tgagggcgac cagcttgccg
     9241 tccggtgccc cggccttgtc gcggtctgtg cgggggccgt gttcgaagac gggggtgagg
     9301 ccccccgggc cgggggcgag cgtgggcgtc cgggaggggg acggggcggc gggggccgag
     9361 ggcgcggggc ggggggcctg acgccctgcc tcggtggttc cgcagccggc cagtgcggcg
     9421 cccaggacac agagcgcggt cgcgcgtcgt agtcgtcgga gagtgatcac cgcacgaaca
     9481 taggagtgag atgttcggtt tgtatgacta tcaggggtga cgtgccgccc ccgtcacccg
     9541 cccggcccac gtaccggtgt cagatgtccc gctgctccag cggcttcgtc gccgggccct
     9601 cgacgacctt gccgtccgtg tcgaagcggg agccgtggca ggggcactcc caggcgcgtt
     9661 cggccgcgtt gaagtcgacg aggcagccca ggtgggtgca gcgcggggag accgcgtgca
     9721 gcgcgccctc ctcgtcccgg tagaccgcga cccggtcgcc gcccgcccgg accaccgcgc
     9781 cctcgcccgg cggcagcgcc tccaccggag gggcggaccg cagccggtcg ccgacgaagt
     9841 gccgggccac ctcggcctgc gtcctgagca gcgccggcgc ctcccgcacc gccgtgcgca
     9901 gccgccgcgg gtcgtacagt ccgctccacg cgcactcctc gccgctgatc tgcgcggtga
     9961 gcagccggcc cgccatcatg ccgccgctca gaccccagcc gccgaagccg gtggcgacgt
    10021 acgcgtgacg ggcaccctgg tgcagcgggc cgaccatcgg cacggtgtcg gtggggtcga
    10081 tgtcctgcgt ggcccaggcg tgggtgcggt cggcaccggg gaagtgctcg tccgcccagg
    10141 cggccagggt ctcgaagcgc tcccgggtgt cgcccgtgcc cggcgtgaag tgctcgccgg
    10201 tgacgatcag cagccggcgg cccgggtcgg tcaggggcgc cgtgcgcacc gagcgggtgt
    10261 tctcctccgg cgtgatgtac atgccgtcga cgtcgcggtc ggcgtcgacg gtcccagcga
    10321 cgaccagttc gcggcgcggg gagaggcggg cgaagagcag ggcgcggtcg aagaccgggt
    10381 agtgcgtggc gaccacgacg tcccgggcgg tgaccgtcgc cccggcctcc gtcctcaccc
    10441 ggcagggctc gccctcgtcc aggccctgga cggtggtgtc ctcgaagatc cgcccgccgc
    10501 gcgcctccag atccgcggcg agggccagca ggtacttgac ggggtggaac tgggcctggc
    10561 cggtcacctt cacggccccc gccaccggga acggcagccc ggtctccgtc acgaacgacg
    10621 cgggcagccc cgcctccttc gccgcggccg cctcggcgcg cagctcctcc acgcggcccg
    10681 ggtcccggac gtaggtgtac gcgtcgcgcg tctcccagtc gcagtcgatg cccagctcgg
    10741 cgacgatccc ggcggcacgc tcgatcgcct cggactgcga gcgggcgtac agccgggcgc
    10801 cctccgggcc ccgggtgcgg cgcagcttgt cgtagaccag ggtgtgctgc gcggtcagct
    10861 tggcgctggt gtacccggtg acgccggcgg cgacccgccc ggcctccagg accgcgacac
    10921 cgcgcccggc ccgcgccagc tcccaggcgg tgctgagccc cgcgacccct gcaccgatca
    10981 cggcgacatc gacgtcgagg tcctccgcga gcgcgggacg gggcgcaccg cccggtgccg
    11041 tctgaagcca gtacgagccg ttgacctgca cgttctgagt catgcggccc gagtaccccg
    11101 gggccgccgc ttcagtggcc ggaccgtgcg ccccgtggac gccgccgtgc cgccgccgct
    11161 acctgcgcca gggacccgtc accgcgaagg tggtgccggg cgtgtagcag ttgacgtaca
    11221 tcgtgtcgcc gtcgggggag aaggtgacgc cggcgaactc accccactcg ggctcctcct
    11281 cggtgccgat gttctgggcg ccgcgggcca tggcgtacac ctcgccgcgc cgggtgacgc
    11341 cgaagacgtg ctgggcgccg ttgccgtc
//
