LOCUS       NZ_CP115937            10972 bp    DNA     linear   CON 13-JUL-2024
DEFINITION  Streptomyces goshikiensis strain CGMCC 4.1796 plasmid punmamed1,
            complete sequence.
ACCESSION   NZ_CP115937
VERSION     NZ_CP115937.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces goshikiensis
  ORGANISM  Streptomyces goshikiensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 510574)
  AUTHORS   Mo,P.
  TITLE     The genome sequence of Streptomyces sporoverrucosus CGMCC 4.1796T
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 510574)
  AUTHORS   Mo,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) College of Life and Environment Science,
            Hunan University of Arts and Science, No. 3150 Dongting Avenue,
            Changde, Hunan 415000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 8.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 184.03x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_027942015.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 07:01:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,266
            CDSs (total)                      :: 7,170
            Genes (coding)                    :: 7,009
            CDSs (with protein)               :: 7,009
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 55 of 162
            Pseudo Genes (incomplete)         :: 131 of 162
            Pseudo Genes (internal stop)      :: 14 of 162
            Pseudo Genes (multiple problems)  :: 36 of 162
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:49:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 335729
            Orig. end                         :: 346701
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115937.1.
            Bacteria and source DNA available from Mo Ping.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10972
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="2"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10972
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10972
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5972](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5972)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    138..152
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.24"
                     /tool="antismash"
     misc_feature    763..783
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.17"
                     /tool="antismash"
     misc_feature    complement(1799..1814)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.5"
                     /tool="antismash"
     misc_feature    2427..2439
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.24"
                     /tool="antismash"
     gene            complement(2455..3513)
                     /db_xref="GeneID:91413971"
                     /locus_tag="PET44_RS33780"
                     /old_locus_tag="PET44_33780"
     CDS             complement(2455..3513)
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (109-349). E-value:
                     1.6e-19. Score: 62.1. Matches aSDomain:
                     nrpspksdomains_PET44_RS33780_PKS_KS.1"
                     /codon_start=1
                     /db_xref="GeneID:91413971"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) t2clf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1093: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019635009.1"
                     /locus_tag="PET44_RS33780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PET44_33780"
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="WP_324615578.1"
                     /sec_met_domain="ketoacyl-synt (E-value: 2e-12, bitscore:
                     42.1, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="t2clf (E-value: 3.9e-111, bitscore: 366.5,
                     seeds: 26, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGLGVAAPNGLGADGFWAATRTGTNGIGRITGFDPSPYPVTLAG
                     QVPGLAAEGRPPSRVLPSRLLAQSGRMPRITLVAAGWALADAGVDPEELGALDLGGLDA
                     GGAAVCAASGLDAVAQARRRIREGAGLVVSGAVDASLSPWGWGVQLVSGRLSGSAEPTR
                     AYLPFDAEAAGHVPGEGGAILILESAAAARARGARVYGEISGYGAVSDPRPGSGREPGL
                     RRAIELALADAGAVPGDVDVVFADAAAIPELDRIEAEAITAVFGPCGVAVTAPKTMTGR
                     LCSGAAPLDLAAALLAIEEGLIPPTVNVRLSDAYQLDLIGPRPRTAEVRTALVLARGHG
                     GFNSAVVVRAVD"
     aSDomain        complement(2467..3186)
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PET44_RS33780_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="1.60E-19"
                     /label="PET44_RS33780_PKS_KS.1"
                     /locus_tag="PET44_RS33780"
                     /protein_end="349"
                     /protein_start="109"
                     /score="62.1"
                     /tool="antismash"
                     /translation="CAASGLDAVAQARRRIREGAGLVVSGAVDASLSPWGWGVQLVSGR
                     LSGSAEPTRAYLPFDAEAAGHVPGEGGAILILESAAAARARGARVYGEISGYGAVSDPR
                     PGSGREPGLRRAIELALADAGAVPGDVDVVFADAAAIPELDRIEAEAITAVFGPCGVAV
                     TAPKTMTGRLCSGAAPLDLAAALLAIEEGLIPPTVNVRLSDAYQLDLIGPRPRTAEVRT
                     ALVLARGHGGFNSAVVVR"
     CDS_motif       complement(2974..3012)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PET44_RS33780_0001"
                     /evalue="9.00E-03"
                     /label="PKSI-KS_m4"
                     /locus_tag="PET44_RS33780"
                     /protein_end="180"
                     /protein_start="167"
                     /score="9.5"
                     /tool="antismash"
                     /translation="FDAEAAGHVPGEG"
     misc_feature    3706..3721
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.26"
                     /tool="antismash"
     gene            complement(3941..4969)
                     /db_xref="GeneID:91413972"
                     /locus_tag="PET44_RS33785"
                     /old_locus_tag="PET44_33785"
     CDS             complement(3941..4969)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PKS_KR (2-304). E-value: 1.5e-06. Score:
                     20.2. Matches aSDomain:
                     nrpspksdomains_PET44_RS33785_PKS_KR.1"
                     /codon_start=1
                     /db_xref="GeneID:91413972"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018546906.1"
                     /locus_tag="PET44_RS33785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PET44_33785"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_271245345.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 2.4e-08, bitscore:
                     28.3, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTIMITGAAGFIGGHVAREAERRGGLSLRLMSHRRPPAGAAGAG
                     PATAGPDPATAGPGSGWGHGHGPGAVVPGHRTVRADLSDPGSLRGVCEGVDVLLHCASQ
                     IGGTPEANEAVNARGTAALMAEARRAGVSRVVYLSTASVYGRGAFRAARPEDLVRNPGS
                     PTSGSRARAEDAVLAAGGVVLRPHLVLGDGDRWVVPSLARILRALPGTVEGWAARTSVI
                     TAQDLARLLLGAGLAPAGDLTASVYHAAHPVPVTVRTLLGAVAAVAGPAADPAAEDLTA
                     VRARELLAARGIAAQGLDMLTSDHWFDSDPVWRDLRCTPGPDFEAAFKELAEGYRRALL
                     DI"
     aSDomain        complement(4058..4963)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PET44_RS33785_PKS_KR.1"
                     /evalue="1.50E-06"
                     /label="PET44_RS33785_PKS_KR.1"
                     /locus_tag="PET44_RS33785"
                     /protein_end="304"
                     /protein_start="2"
                     /score="20.2"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="TIMITGAAGFIGGHVAREAERRGGLSLRLMSHRRPPAGAAGAGPA
                     TAGPDPATAGPGSGWGHGHGPGAVVPGHRTVRADLSDPGSLRGVCEGVDVLLHCASQIG
                     GTPEANEAVNARGTAALMAEARRAGVSRVVYLSTASVYGRGAFRAARPEDLVRNPGSPT
                     SGSRARAEDAVLAAGGVVLRPHLVLGDGDRWVVPSLARILRALPGTVEGWAARTSVITA
                     QDLARLLLGAGLAPAGDLTASVYHAAHPVPVTVRTLLGAVAAVAGPAADPAAEDLTAVR
                     ARELLAARGIAAQGLDMLTSD"
     gene            complement(5001..5972)
                     /db_xref="GeneID:91413973"
                     /locus_tag="PET44_RS33790"
                     /old_locus_tag="PET44_33790"
     CDS             complement(5001..5972)
                     /codon_start=1
                     /db_xref="GeneID:91413973"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008740559.1"
                     /locus_tag="PET44_RS33790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PET44_33790"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_271245346.1"
                     /sec_met_domain="AfsA (E-value: 7.5e-21, bitscore: 69.0,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSLTALRIPAHHSAPAANCLLTTTVPREYVHRAAVSEVLLTGWG
                     PDPLAGRDQSDSYVVRAQWPRGHSLFAPAGGYQDPLLLIESVRQTGALLAHSEYGVPFG
                     HQFLMWDMSFIATPEALVTSPVPTEVELRTVCRDVVRRGRTIASMRYEVSVWRDGVPLA
                     TASAAFSCTSPAVHRRLRGERPTTSDRTPPPAVDPAVVGRSSAPGNVVLAPAPAGPGDR
                     WELRVDTEHPIFFDHPVDHVPGMVLLESARQAAHAVTGLPDALVLGMASTFERYAELDA
                     PCWIEARAERPDAAGDIAVHVVGTQQDRPVFTADLTLRPSAG"
     misc_feature    complement(5694..5696)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(6079..6092)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 17.76"
                     /tool="antismash"
     misc_feature    6079..6093
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.44"
                     /tool="antismash"
     misc_feature    complement(6303..6317)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.51"
                     /tool="antismash"
     misc_feature    6304..6318
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.61"
                     /tool="antismash"
     gene            6467..7258
                     /db_xref="GeneID:91413974"
                     /locus_tag="PET44_RS33795"
                     /old_locus_tag="PET44_33795"
     CDS             6467..7258
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:91413974"
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018557968.1"
                     /locus_tag="PET44_RS33795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PET44_33795"
                     /product="response regulator transcription factor"
                     /protein_id="WP_008740558.1"
                     /transl_table=11
                     /translation="MNRQAADVLEPRSEQIRPQRTLLSQPGHFPPPRVERPAAAAEPAL
                     RVLVVENEARAADTLVVGLRRHGYLAESVATGALALQAHGDADLILLDLDLPDLDGLEV
                     CRAIRAVGDTPLIAVTARGSELDRVLGLQAGADDYLVKPYGFRELLARMEAVMRRARPR
                     PQASRVITHGPLRIDAGTREVLLAGRPVELTRKEFDLLHLLASQPEAVISRRQLMTQVW
                     DDTWSHRGRTIDTHVSSLRGKLGASGWIVTVRGVGFRLGRP"
     misc_feature    complement(7359..7369)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     gene            7364..8005
                     /db_xref="GeneID:91413975"
                     /locus_tag="PET44_RS33800"
                     /old_locus_tag="PET44_33800"
     CDS             7364..8005
                     /codon_start=1
                     /db_xref="GeneID:91413975"
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137541.1"
                     /locus_tag="PET44_RS33800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PET44_33800"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_073776722.1"
                     /transl_table=11
                     /translation="MAQQERGNRSRRSILEAAARVFDERGYDAASTNEILASTGLTRGA
                     LYHHFPSKEAIASALVAAQSEALVVPDQPVKLQAVIDLTLEFARRLQHDPVLRASVRLA
                     VEQTSFSRPAQTPYEQSNATILDLLRQAREQGEVLPGVDLQEATSTIVGAFTGLQVMSQ
                     VYSNRQDLPDRVSALWRFLLPGLATPGMLARLRTAPLPQTAPQAAPQTAP"
     gene            complement(8021..8608)
                     /db_xref="GeneID:91413976"
                     /locus_tag="PET44_RS33805"
                     /old_locus_tag="PET44_33805"
     CDS             complement(8021..8608)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:91413976"
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /locus_tag="PET44_RS33805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PET44_33805"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_008740556.1"
                     /transl_table=11
                     /translation="MQKRSEQTRQALVSAAARIIADGRPADAGLVNICRTAGVSRGALY
                     HHFSSTTELTAAVYSQAFERATTLAEEAFEGPAESAAARFCSSLCTALRDEELVRAGTQ
                     LAADGSAGPPRLRDDALDLVRRHLVSAGGELAELADLAVVVTAGLESLGRTDGTWWEPR
                     TSERIWDLLRPLFGPRGPARGGSPLGAAGRQD"
     misc_feature    8700..8709
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     misc_feature    complement(8700..8713)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.59"
                     /tool="antismash"
     misc_feature    complement(8779..8792)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: medium, score: 20.48"
                     /tool="antismash"
     misc_feature    complement(8786..8802)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.19"
                     /tool="antismash"
     gene            complement(8885..10546)
                     /db_xref="GeneID:91413977"
                     /locus_tag="PET44_RS33810"
                     /old_locus_tag="PET44_33810"
     CDS             complement(8885..10546)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:91413977"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1138: FAD linked oxidase domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011030784.1"
                     /locus_tag="PET44_RS33810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PET44_33810"
                     /product="FAD-binding protein"
                     /protein_id="WP_008740555.1"
                     /transl_table=11
                     /translation="MANIARRALFRGAAATVSAVAGSAALGAVTAAPAVADEVVGEGQD
                     AMGARTTAPTGATITPNDPRYAQLTTGNNQRYVAAPSYVKMIKSAPDAAAAITAAYRAG
                     KRVSVRSGGHCFSNFVCNPDVQVILDCSEMTYVGYDPAMRAFAVEPGARLLNVYEALYK
                     GWGVTLPGGVCYGVGAGGHIAGGGYGLLSRRNGLVVDHLYAVEVVTVDASGTARIVRAT
                     REPNDPNRDLWWAHTGGGGGNFGVVTRYWLRSPNAPGGSAATPENQLVSPPSHVLMHIQ
                     GIPWSQLDEAKFSRLVKNFGAWHEANSAPNSPLRNLSSILNVYSKASGMLNLVTQVDAG
                     VPDAAAMLDTYLNQILDGVTTARAASRKLPWLQAASLIGTINTTLNDPTLRGAHKSAYM
                     RTNFTDAQLASLYASMTAADYTNPRAMLILFSFGGQVNAVAPEATAIPQRSSAFKMLYQ
                     TFWNSAAEDSTHVAWLRTLYANMHAATGGVPGLDGQTDGCYINYPDTDMADPAQNTSGV
                     PWQRLYYKGNYARLQQVKARWDPSDYFRHSMSVKQV"
ORIGIN
        1 cgggctgttt gatgccgatg ttggcgacgt tgtagccgtt gttggtgagg atgatgtcga
       61 cgaggttctt gccgatgtcg tggacgtcgc cgcggacggt ggcgagcacg atggtgccct
      121 tgccgtccgc gtcggtcttc tccatgtgcg gttcgaggtg ggcgacggcc gtcttcatga
      181 cctccgccga ctggaggacg aaggggagct gcatctggcc ggagccgaat agctcgccga
      241 cgaccttcat gccctccagg agggtgtcgt tgacgatgtc gagggccggg cgggtctgga
      301 gcgcctcgtc gaggtccgct tccaggccgt tcttctcgcc gtcgatgatc cggcggcgca
      361 ggcgttcctc caggggcagc gccatcagtt cctcggcgcg gccggccttc agcgacttgg
      421 tgttgacgcc ctcgaagagg gccatcagct tctggagcgg gtcgtagccc tccgcccgcc
      481 ggtcgtagat caggtcgagg gcggtgccca cctgctcctc gtcgaagcgg gcgatgggga
      541 ggatcttgct ggcgtggacg atcgcggagt ccaggccggc cttgacgcac tcgtcgagga
      601 agacggagtt gaggaggata cgggcggccg ggttgaggcc gaaggagatg ttcgagaggc
      661 cgagggtggt ctggacgtcc gggtggcggc gcttgagctc gcggatcgcc tcgatcgtgg
      721 cgatgccgtc ccctcgggac tcctcctggc cggtgcagat ggtgaaggtc agggtgtcga
      781 tgaggatgtc ggattcgagg atgccccagt tctcggtgag gtcctggatc agccgctccg
      841 cgatctccac cttgtgctcg acggtacggg cctggccctc ctcgtcgatg gtcagcgcga
      901 tcagcgcggc gccgtgctcc cgcgccagcc gggtgacctt ggcgaagcgc gagtccgggc
      961 cgtcgccgtc ctcgtagttg acggagttga tgacggcgcg gccgccgagc ttctccaggc
     1021 ccgcctggat gacgggaacc tcggtggagt ccaggacgat cggcagcgtc gaggcggtcg
     1081 cgaaccggcc ggcgagttcc gccatgtcgg cgacgccgtc ccgcccgacg tagtccacgc
     1141 acaggtccag catgtgcgcg ccctcgctga tctggtcccg gaccatctcc acgcagtcgt
     1201 cccagcgggc ctccagcatg gcctcgcgga actgcttcga cccgttggcg ttggtccgct
     1261 cgccgatcgc catgtacgag gtgtcctgcc ggaacggcac cgtctggtac agcgaggccg
     1321 caccgggctc cgggctcggt tcgcgcaccg ccggggccag cccgcggacc cgctcgacga
     1381 cctggcgcag gtgctccggg gtcgtgccgc agcagccgcc gaccagggag agaccgtact
     1441 cgcggacgaa ggactcctgg gcgtcggcga gctcgctcgc cgacagcggg tagtgcgcgc
     1501 cgtccttcgt cagcaccggc aggcccgcgt tcggcatgca ggacagcggg atccgggagt
     1561 tgcgggcgag gtagcgcagg tgctcgctca tctccgcggg gccggtggcg cagttcagcc
     1621 cgatcatgtc gatgccgagg ggttcgaggg cggtcagggc cgcgccgatc tcggagccca
     1681 gcagcatggt gccggtggtc tcgacggtga ccgagcagat cagcgggacc gagaggccga
     1741 gggcctccat cgcccgccgg gcgccgatga cggaggactt ggtctggagg aggtcctggg
     1801 tggtctccac cagcagcgcg tccgcgccgc ccgtcaggag cccctccgcg ttcgcctggt
     1861 aggcgtcgcg gatcgtcgcg tagtcgatgt ggccgagggt gggcagcttg gtgccggggc
     1921 ccatcgagcc gaggacccag cgctgctgcc cggtggcggc ggtgaactcg tccgcggcct
     1981 cgcgggcgat ccgggcgccc gcctccgaca gctcgaagtt gcgctccgcg atgtcgtact
     2041 cggcgagggc ggcgaagttc gcgccgaagg tattggtctc cacacagtcc acgccgacgg
     2101 agaaatacgc ctcgtggacc gtccggacga tatcggggcg ggtgacgttc agaatctcgt
     2161 tgcagccttc gaggttctgg aaatcatcga gggtggggtc ctgcgcctgg agcatggtgc
     2221 ccatggcgcc gtcggcgacc accacgcggg tcgcgagcgc ctcacggagg gcattggagc
     2281 ggttccggct gtcaccggaa agggttggca gcgaggccat ggaaaagctc ctgttcaacg
     2341 agagggccgc tcatagagga tcttgggtca actctccggt cacaagccgc gtcagggtca
     2401 gaggaatgtt aaattccgca agaatcaagc aatttgaaag aacggcgata accgtcagtc
     2461 cacggcgcgg accaccacgg ccgagttgaa gccgccgtga ccgcgcgcca ggaccagcgc
     2521 ggtgcgcacc tcggcggtgc gcggccgggg tccgatcagg tccagctggt acgcgtcgga
     2581 cagcctcacg ttgaccgtgg gcgggatcag gccctcctcg atggccagca gcgcggccgc
     2641 caggtcgagc ggggcggcgc ccgaacacag ccggcccgtc atcgtcttgg gcgcggtgac
     2701 cgcgacgccg caggggccga acaccgcggt gatggcctcg gcctcgatcc ggtccagctc
     2761 ggggatggcg gccgcgtcgg cgaagaccac gtccacgtcg ccgggtacgg ctcccgcgtc
     2821 ggccagggcc agctcgatcg cccggcgcag gcccggctcg cgcccgctgc cgggccgggg
     2881 gtcggacacc gctccgtagc cggagatctc cccgtagacg cgggcgccgc gcgcccgggc
     2941 cgccgcggcg gactccagga tcaagatcgc gccgccctca ccggggacgt gtccggcggc
     3001 ctcggcgtcg aaggggaggt aggcgcgggt gggctccgcg ctgccgctca gccggccgct
     3061 gacgagctgg acgccccagc cccaggggct gagcgaggcg tccacggcgc cggagacgac
     3121 caggccggcg ccctcgcgga tgcggcggcg ggcctgcgcg acggcgtcga gcccgctcgc
     3181 cgcgcagacc gcggcgccgc cggcgtccag cccgccgagg tcgagcgcgc cgagctcctc
     3241 ggggtccacg cccgcgtcgg cgagcgccca gcctgcggcg acgagcgtga tccggggcat
     3301 ccggccgctc tgcgccagca gccggctcgg cagtacccgg ctcggcggcc gcccctcggc
     3361 ggcgaggccg gggacctggc cggcgagggt caccgggtac ggggaggggt cgaagccggt
     3421 gatccggccg atgccgttcg taccggtgcg ggtcgcggcc cagaagccgt ccgcgccgag
     3481 tccgttgggg gcggcgacgc ccaggccggt cacgaccacc gaggtggccg tgccgcgttc
     3541 ctcccgggac gcggcggccg ggtggcacag ggggatccgg cccggccgtc gccggcagcc
     3601 ggggccgtgg tggaaggccg ggcgcgaacg tccggcgggc cgtaagcggg atcgcgttcc
     3661 gggccgggag tgggcggatt cgcggtcggc gggacgccgc gatccggtga agggtttcac
     3721 ggcaccgatt gccggtgggg ggtccgtccg gaccactgtc agcgtgccgt gcataccttc
     3781 cctctccttt ggcccaccgc gaaaagaatg ccggagcacg atgtacagcg tgttccgtcc
     3841 cgggccgcgg ttcaatacgg ggccgtgcag aactcggtca ggtttccgta aggccattgc
     3901 atacgtgtcg ggttcagttc aggagaagtc caggggaatg tcagatgtcg agcagggcgc
     3961 ggcggtagcc ctcggcgagt tccttgaacg cggcctcgaa atccgggccg ggcgtacacc
     4021 gcaggtcccg ccagaccggg tcgctgtcga accagtgatc gctcgtcagc atgtcgagcc
     4081 cctgggccgc gatgccccgg gcggcgagca gctcgcgggc gcgcaccgcc gtgaggtcct
     4141 cggcggccgg gtccgccgcc gggcccgcga cggcggcgac cgcgccgagc agggtgcgga
     4201 cggtgacggg cacggggtgg gcggcgtggt agacggacgc cgtcaggtcc ccggccgggg
     4261 cgaggccggc gccgagcagg agccgggcca ggtcctgcgc ggtgatgacg gacgtacggg
     4321 cggcccagcc ctcgacggtg cccggcaggg cccgcaggat ccgggccagg ctcgggacca
     4381 cccagcggtc gccgtcgccg aggacgaggt gcgggcgcag gacgaccccg cccgccgcga
     4441 ggacggcgtc ctcggcgcgg gcgcgcgagc ccgaggtggg cgagcccggg ttgcggacga
     4501 ggtcctcggg ccgggcggcg cggaaggcgc cgcggccgta cacggaggcg gtgctgaggt
     4561 agacgacgcg ggagaccccg gcgcgccggg cctcggccat cagggcggcc gtaccgcggg
     4621 cgttgacggc ctcgttggcc tcgggggtgc cgccgatctg cgaggcgcag tgcagcagta
     4681 cgtcgacccc ctcgcagacg ccccgcagcg agccggggtc cgagaggtcg gcgcgcacgg
     4741 tccggtggcc ggggaccacg gcgccggggc cgtggccgtg gccccagcca gagccggggc
     4801 cggccgtggc ggggtcaggg ccggccgtgg cggggccggc ccccgccgcg ccggccggcg
     4861 gtcttcggtg ggacatcagc cgcagggaca ggccgccccg gcgctcggcc tcgcgggcga
     4921 cgtgtccgcc gatgaatccg gccgctcccg tgatcatgat ggtgggcacg cgcgctctcc
     4981 tggtggtggt gatggcgggc tcagcccgcg ctggggcgca gggtgaggtc ggcggtgaag
     5041 accggccggt cctgctgggt gccgacgacg tggacggcga tgtcccccgc cgcgtcgggg
     5101 cgttcggcgc gggcctcgat ccagcagggg gcgtcgagtt cggcgtaccg ctcgaaggtg
     5161 ctcgccatgc cgaggacgag ggcgtccggc agtccggtga cggcgtgggc ggcctggcgg
     5221 gccgactcca gcagcaccat gccggggacg tggtcgacgg ggtggtcgaa gaagatcggg
     5281 tgctcggtgt cgacccggag ctcccaccgg tcgcccggac ccgccggggc gggggcgagg
     5341 acgacgttgc cgggggcgga ggacctgccg accacggccg ggtcgacggc cggcggcggg
     5401 gtgcggtccg aggtggtggg gcgctcgccg cgcagccgcc ggtgcacggc cgggctggtg
     5461 cagctgaagg cggcggaggc ggtggcgagc ggcactccgt cgcgccacac cgagacctcg
     5521 tagcgcatgc tcgcgatggt gcggccgcgg cggacgacgt cgcggcacac ggtgcgcagc
     5581 tcgacctcgg tgggcacggg gctcgtcacg agcgcctcgg gggtggctat gaaggacatg
     5641 tcccacatca ggaactggtg cccgaagggg accccgtact cggagtgcgc gagtaaggcc
     5701 ccggtctggc ggaccgactc gatcagcagc agcgggtcct ggtagccgcc ggccggggcg
     5761 aagagggagt ggccgcgcgg ccactgtgcc cgcacgacgt aggagtcgct ctggtcgcgg
     5821 ccggcgagcg ggtcgggtcc ccagccggtc agcagtactt cgctgacggc cgcgcggtgc
     5881 acgtactccc ggggcaccgt ggtggtgagc aggcagttcg ccgccggagc gctgtggtgg
     5941 gcggggatac ggagagcggt gagggacgac atgcgctgta ctcctgttct gcttgaagag
     6001 caggggtgag ccacgcacac gtcggcagcc ccccgctgtg atcgcttgca gacctctaag
     6061 atacagacag gtcggttctt ttcttaggaa atcttcgagt tttccgtaac aaccgctgac
     6121 ccggaccgac ggaatcccgc caagaggtcg gttcctcccg cgcgagcgcc gccggaaccc
     6181 ttgactccgg ccccaactcc actggaacag acagcactgc cgggtttacg cgcccgcaac
     6241 gcgggtcgcc gcacctctcc tccgatcatg gaaaacgacc aaaccgatgg cccaaaccat
     6301 cctattttca agatacttgc tggtcagaga ggcctcctga cgacccgacc aacgccaacg
     6361 cgcggagccc gcgctcccgt tgacaatcag acaagtctgt ttggtatctt cattcagcac
     6421 gagcgttcag cagtccgatg gcgcgactgc gcctcaggag gagcagatga atcggcaggc
     6481 cgcggatgtc ctcgaacccc gctcggagca gatccgcccc cagcgcaccc tgctttccca
     6541 gccgggccac ttcccgccgc cgcgggtgga gcggcccgcc gccgcggccg aaccggccct
     6601 gcgggtcctc gtcgtcgaga acgaggcgcg cgcggccgac accctggtgg tggggctgcg
     6661 ccggcacggc tacctggccg agagcgtggc caccggcgcc ctcgccctcc aggcgcacgg
     6721 cgacgccgac ctgatcctcc tcgacctcga cctgcccgac ctcgacggcc tggaggtctg
     6781 ccgcgccatc cgcgccgtcg gcgacacccc gctgatcgcg gtcaccgcgc gcggcagcga
     6841 gctcgaccgg gtgctcgggc tccaggccgg ggcggacgac tacctcgtca agccgtacgg
     6901 tttccgcgaa ctgctcgcgc ggatggaggc ggtgatgcgc cgggcccgcc cccggccgca
     6961 ggccagccgg gtcatcacgc acgggccgct gcgcatcgac gccggcaccc gcgaggtcct
     7021 gctggccggc cggccggtgg agctgacgcg caaggagttc gacctgctcc acctgctcgc
     7081 ctcgcagccg gaggcggtca tctcccgccg ccagctgatg acgcaggtgt gggacgacac
     7141 ctggtcgcac cgcggccgca ccatcgacac ccacgtcagc agcctgcgcg gcaagctcgg
     7201 ggcgagcggc tggatcgtca cggtgcgcgg cgtcgggttc cgcctcggcc ggccgtagcc
     7261 cctcccgggc gcccgggagg ggccgcccgg ggcccctcgt tccgcagacc gatacaattc
     7321 cgacaggtcg gtttggttcc agtcagtaac gcaaggacgt ggcatggcac agcaagaacg
     7381 cggcaaccgg tcgaggcggt cgatcctgga ggccgccgcg cgcgtcttcg acgagcgcgg
     7441 gtacgacgcc gcgagcacga acgagatcct cgcgagcacc ggtctgacca ggggggcgct
     7501 ctaccaccac ttcccctcga aggaggcgat cgcctccgcg ctggtggccg cgcagagcga
     7561 ggcgctggtg gtgcccgacc agcccgtcaa gctccaggcc gtcatcgacc tcacgctcga
     7621 gttcgcgcgc cggctccagc acgacccggt gctgcgggcg agcgtacggc tggcggtgga
     7681 gcagacctcc ttctcgcgcc cggcgcagac cccgtacgag cagtcgaacg cgaccatcct
     7741 cgacctgctg cgccaggcgc gggaacaggg tgaggtgctg cccggcgtgg acctccagga
     7801 ggccacgtcc acgatcgtgg gggccttcac cggcctccag gtgatgtcgc aggtgtacag
     7861 caaccggcag gacctgccgg accgggtctc cgcgctgtgg cggttcctgc tgcccggact
     7921 ggccacgccc ggcatgctcg cccggctgcg cacggcgccg ctgccgcaga ccgcaccgca
     7981 agccgcgccg cagaccgcgc cgtagccccc gcgcgccccg tcagtcctga cggcccgccg
     8041 cccccagggg gcttcctccc cgcgccggcc cccgggggcc gaacaggggg cgcagcaggt
     8101 cccagatccg ctccgaggtg cgcggttccc accacgtgcc gtcggtgcgc cccagcgact
     8161 ccagcccggc ggtgaccacc acggcgaggt cggccagttc ggccagttca ccgccggccg
     8221 agaccaggtg acggcggacc aggtccaggg cgtcgtcccg cagccgcggc ggcccggcgc
     8281 tgccgtccgc cgccagctgc gtgcccgccc gtacgagttc ctcgtcgcgc agcgcggtgc
     8341 acagcgagga acagaaccgg gcggcggccg actcggcggg gccttcgaag gcctcctccg
     8401 ccagggtcgt ggcccgctcg aaggcctggc tgtacacggc ggcggtcagc tccgtggtgg
     8461 aggagaagtg gtggtagagc gctccgcggc tcactccggc ggtgcggcag atgttcacca
     8521 gcccggcgtc ggcgggccgg ccgtccgcga tgatcctcgc cgcggcggag accagggcct
     8581 ggcgggtctg ttcggaacgc ttctgcatgg cgcggcacct gcatctctct cttcacggca
     8641 cacagaagcg cgggggaccg ccgggccgtc ggcactacac cgaatcatac aaccctgcct
     8701 gttttttgaa acccctcgcc caacgagagc ccctttacag cccctttgac ctccccggat
     8761 gcttggtatt cgctgatttc gcgcagagtg attcagtaac aaccagacaa gtctgttcgt
     8821 tatgacggcg tcggccggcg ccgtgacctt ccctcaccgg ccgacgcggg agcactccgg
     8881 ccggtcagac ctgcttcacg gacatcgagt gccggaagta gtcggagggg tcccacctgg
     8941 ccttgacctg ctgcagccgc gcgtagttgc ccttgtagta cagccgctgc cacggcacgc
     9001 cggaggtgtt ctgcgccgga tcggccatgt cggtgtccgg gtagttgatg tagcagccgt
     9061 cggtctggcc gtcgaggccc gggaccccgc ccgtcgccgc gtgcatgttc gcgtacaggg
     9121 tgcgcagcca ggcgacgtgc gtgctgtcct cggccgcgct gttccagaac gtctggtaga
     9181 gcatcttgaa ggccgagctg cgctgcggga tcgccgtggc ctcgggcgcg accgcgttca
     9241 cctggccgcc gaaggagaac aggatcagca tggcgcgcgg gttcgtgtag tccgccgccg
     9301 tcatcgacgc gtacagcgag gcgagctgcg cgtccgtgaa gttggtgcgc atgtacgccg
     9361 acttgtgggc cccgcgcagc gtggggtcgt tgagcgtggt gttgatggtg ccgatgaggc
     9421 tggcggcctg gagccacggc agtttgcggc tcgcggcgcg cgcggtggtg accccgtcga
     9481 ggatctggtt gaggtaggtg tcgagcatcg cggccgcgtc ggggacgccg gcgtccacct
     9541 gggtgaccag gttcagcatc ccgctggcct tggagtagac gttcaggatg ctgctcaggt
     9601 tgcgcagcgg cgagttgggg gcgctgttcg cctcgtgcca ggcgccgaag ttcttcacga
     9661 gccggctgaa cttggcctcg tccagctggc tccagggaat gccctggatg tgcatcagga
     9721 cgtgggaggg cggggagacg agctggttct ccggggtggc cgcggagccg ccgggggcgt
     9781 tcggggagcg cagccagtag cgcgtgacca cgccgaagtt gccgccgccg ccgcccgtgt
     9841 gcgcccacca caggtcccgg ttggggtcgt tcggctcgcg ggtggcccgc acgatgcggg
     9901 cggtgccgga ggcgtcgacg gtgacgacct cgacggcgta gaggtggtcg acgaccaggc
     9961 cgttgcggcg ggagaggaga ccgtagccgc cgccggcgat gtggccgccc gcgccgacgc
    10021 cgtagcagac gccgccgggg agggtgacgc cccagccctt gtacagggcc tcgtagacgt
    10081 tgaggagacg ggcgccgggc tcgacggcga aggcccgcat ggccgggtcg tagcccacgt
    10141 acgtcatctc ggagcagtcg aggatgacct ggacgtccgg gttgcagacg aagttgctga
    10201 agcagtggcc gccgctgcgc accgagaccc gcttgccggc ccggtacgcg gcggtgatgg
    10261 cggcggccgc gtcgggcgcc gacttgatca tcttcacgta cgagggcgcg gcgacgtacc
    10321 gctggttgtt gccggtggtg agctgcgcgt accgcgggtc gttcggggtg atcgtggccc
    10381 cggtcggcgc cgtcgtacgc gctcccatgg cgtcctggcc ctcgcccacg acctcgtcgg
    10441 cgacggccgg ggcggcggtg acggcgccga gcgccgcgct gccggcgacg gcggagacgg
    10501 tggcggcggc gcctctgaag agcgcccgtc tggcgatgtt ggccatgctg gtttccccct
    10561 tggatgtgac ggtgtgtggg aggggccccc ggctgctgcc gggggcccgt ccggggtggg
    10621 agtcggatgc gggacgtcgg gaacgggaag ggaccgccgg ggcccccggt ggccgggggc
    10681 cggggcggct agcccttggg ggccgggccg ttgcccagcg acgaggcgcc gctggcgtcg
    10741 atgacctggc cggagaccca gccggcctcg ccggacgcga ggaacgcggc gatgcccgcc
    10801 acgtccgccg gggtgccggt gcggccgaac gccgaccagc tctcggccgc ggcccgcatc
    10861 tgcgggtacg ccagccaggg gttgaagtcg gtgtcgatga tgccgggggc gatcgcgttg
    10921 acggtgatcc cgcggggcgc gagcgcctcg gcgagggtca gggtcagggt gt
//
